Prupe.5G141900_v2.0.a1

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0000847
Physical Location & Seq
Reverse (-)
13166880 .. 13170277
3398 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G141900.2

Sequence Viewer

Length: 1404 bp
ATGGTGGCTTACTACATAATATTTCTGTTAGTCTTCAACATGACCAGGGTAAGAGATGAAGCGTTTGAAGACAAAGGACCGGCTGTGAACTACTTAGATGCCAACTATTTTCATGCTCCTGCCTCTCAAAATCGTGCGTATGACCGCGTTTCTTCTCCTGCTGATAACATAAGGAGAGATGCTGCTGAGGATCAAGGGTTGCGTAAGGACCATCTGGGTCTGGAAGAATTTTCTGGTTATATATTTATGTGTAATGGAAGAACAAAACCAGAATGCTACCTGCACCGTGTTTTTGGACTTCCCGCTGGAAGAAAGGAAGTTATAGAGAAGATTAGGCCAGGCATGAAGCTGTTTCTATTTGATTATAATGTGAAGTTTCTTTATGGGGTATATGAAGCAACCACTGCTGGAAAGTTGAACTTGGAAAGAACGGCTTTTGGTGGAAACTTTCCTGCACAGGTGAGATTCAAAATTTACAGGGAATGCCTCCCCTTGCCAGAGAGTGATTTTAAACGTGCTATTCAAGACAACTACAAGAGAGGTTCCAATAAGTTTAATACAATTCTCAATAGCCAACAAGTAAGCCATTTGTTATCACTGTTTCATCCTATTTCCATGCAATCATCCACCCAAAACGACAGTTCCTATATGAAGGATCAATTTCAGACCGGTGCAAGGCTGCCTTCCTCAGGCAGTACATACTTCAGTTCAATGCATTCCACTCCACAAGTGTTAGTGCCTCAACATGTTCCACAGGGAGTCCATCAGCAGTGGGATAGTAGTGGATCCACAGGAAACATGGGTATTGTCCATTATTCTGTGGGTTCACAACCAATAGTGCCGGCACAACCTATGAAGGATCAGTTTCAGTCCTCAGTAAGCCTGCCTTCCATGAAGGATCCAAATGCAGCCTGGGCTTCACCAATGTTGGAGCCTCGGTATATTCAACCGAGCGTCCTGCACCCACAATCCCCTAGTTATGGATACACAATGAACATGGGCTACTTTTATCCTGCTGTGCAGCCACAAGCTATGCCAGCTTCAAGTCAGACATATTATTCTGCTGAATTTGGGCAGCATCACTTGGCTGAAGTAACTGCCCAGCCAGCACCCAAATCATATGAAAGCTATTACAGGTATGAGGCTGCACAGGATATGGTTCTTACTGATCAACACACGGGCTTCGGGCATGGTTACAGTCAGTCATCTCTGCAGACGGATGGGGAAACTATTTTACAGCAGGAGAATGCTGCTGAACACCACAATCTCCACCAAGTGCCTACAGCCCCACATGCTCCACCATCTATGCAACAACATACGTTCACATCAGAACAGCCTGGGCTTGCAGCAGGGACAGTTCCGATGCCGTCATCAAATTCCTGGACAGGGGCCAGCCCAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

468

Amino Acids

52.23

Weight (kDa)

6.43

Isoelectric Point (pI)

55.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 366
Acc36I ACCTGC 1 cut(s) 288
AccII CGCG 1 cut(s) 147
AciI CCGC 2 cut(s) 145, 303
AclWI GGATC 7 cut(s) 198, 663, 780, 793, 867, 893, 906
AcsI RAATTY 4 cut(s) 227, 471, 1067, 1375
AcuI CTGAAG 2 cut(s) 688, 1110
AdeI CACNNNGTG 1 cut(s) 1276
AfaI GTAC 1 cut(s) 697
AfiI CCNNNNNNNGG 4 cut(s) 675, 689, 980, 1386
AflIII ACRYGT 1 cut(s) 745
AgeI ACCGGT 1 cut(s) 668
AgsI TTSAA 8 cut(s) 37, 68, 418, 469, 524, 711, 947, 1044
AjnI CCWGG 5 cut(s) 44, 337, 911, 1336, 1379
AluBI AGCT 4 cut(s) 349, 1031, 1040, 1128
AluI AGCT 4 cut(s) 349, 1031, 1040, 1128
AlwI GGATC 7 cut(s) 198, 663, 780, 793, 867, 893, 906
AoxI GGCC 2 cut(s) 335, 1389
ApeKI GCWGC 8 cut(s) 182, 679, 908, 1021, 1075, 1145, 1250, 1346
ApoI RAATTY 4 cut(s) 227, 471, 1067, 1375
AsiGI ACCGGT 1 cut(s) 668
AspS9I GGNCC 3 cut(s) 77, 208, 1389
AsuHPI GGTGA 2 cut(s) 472, 912
AvaII GGWCC 2 cut(s) 77, 208
AxyI CCTNAGG 1 cut(s) 688
BamHI GGATCC 2 cut(s) 785, 898
BbsI GAAGAC 2 cut(s) 25, 75
BbvCI CCTCAGC 1 cut(s) 186
BbvI GCAGC 8 cut(s) 169, 666, 920, 1033, 1087, 1132, 1237, 1358
BccI CCATC 4 cut(s) 219, 771, 1214, 1309
BceAI ACGGC 2 cut(s) 447, 1351
BcgI CGANNNNNNTGC 2 cut(s) 940, 974
BciT130I CCWGG 5 cut(s) 46, 339, 913, 1338, 1381
BciVI GTATCC 1 cut(s) 977
BclI TGATCA 1 cut(s) 1168
BfaI CTAG 1 cut(s) 975
BfmI CTRYAG 2 cut(s) 1211, 1281
BfuAI ACCTGC 1 cut(s) 288
BfuI GTATCC 1 cut(s) 977
BisI GCNGC 8 cut(s) 183, 680, 909, 1022, 1076, 1146, 1251, 1347
BlsI GCNGC 8 cut(s) 184, 681, 910, 1023, 1077, 1147, 1252, 1348
Bme1390I CCNGG 5 cut(s) 46, 339, 913, 1338, 1381
Bme18I GGWCC 2 cut(s) 77, 208
BmgT120I GGNCC 3 cut(s) 77, 208, 1389
BmiI GGNNCC 5 cut(s) 544, 787, 900, 933, 1390
BmrFI CCNGG 5 cut(s) 46, 339, 913, 1338, 1381
BmsI GCATC 4 cut(s) 88, 169, 1087, 1353
BpiI GAAGAC 2 cut(s) 25, 75
Bpu10I CCTNAGC 1 cut(s) 186
BsaJI CCNNGG 4 cut(s) 45, 912, 935, 1337
BsaWI WCCGGW 1 cut(s) 668
BsaXI ACNNNNNCTCC 2 cut(s) 923, 953
Bsc4I CCNNNNNNNGG 4 cut(s) 675, 689, 980, 1386
Bse118I RCCGGY 3 cut(s) 79, 668, 841
Bse21I CCTNAGG 1 cut(s) 688
BseBI CCWGG 5 cut(s) 46, 339, 913, 1338, 1381
BseDI CCNNGG 4 cut(s) 45, 912, 935, 1337
BseGI GGATG 3 cut(s) 604, 623, 1225
BseLI CCNNNNNNNGG 4 cut(s) 675, 689, 980, 1386
BseMII CTCAG 3 cut(s) 177, 702, 888
BseXI GCAGC 8 cut(s) 169, 666, 920, 1033, 1087, 1132, 1237, 1358
BseYI CCCAGC 1 cut(s) 1101
BsgI GTGCAG 5 cut(s) 266, 438, 944, 1040, 1131
Bsh1236I CGCG 1 cut(s) 147
BshFI GGCC 2 cut(s) 337, 1391
BshTI ACCGGT 1 cut(s) 668
BsiSI CCGG 3 cut(s) 80, 669, 842
BslFI GGGAC 1 cut(s) 1366
BslI CCNNNNNNNGG 4 cut(s) 675, 689, 980, 1386
BsmFI GGGAC 1 cut(s) 1366
BsmI GAATGC 4 cut(s) 278, 488, 715, 1252
BsnI GGCC 2 cut(s) 337, 1391
Bsp143I GATC 6 cut(s) 190, 655, 785, 859, 898, 1168
BspACI CCGC 2 cut(s) 145, 303
BspANI GGCC 2 cut(s) 337, 1391
BspCNI CTCAG 3 cut(s) 178, 701, 887
BspFNI CGCG 1 cut(s) 147
BspLI GGNNCC 5 cut(s) 544, 787, 900, 933, 1390
BspMAI CTGCAG 1 cut(s) 1215
BspMI ACCTGC 1 cut(s) 288
BspPI GGATC 7 cut(s) 198, 663, 780, 793, 867, 893, 906
BsrFI RCCGGY 3 cut(s) 79, 668, 841
BssAI RCCGGY 3 cut(s) 79, 668, 841
BssECI CCNNGG 4 cut(s) 45, 912, 935, 1337
BssMI GATC 6 cut(s) 190, 655, 785, 859, 898, 1168
Bst2UI CCWGG 5 cut(s) 46, 339, 913, 1338, 1381
Bst4CI ACNGT 5 cut(s) 287, 600, 641, 1199, 1357
BstAPI GCANNNNNTGC 1 cut(s) 404
BstC8I GCNNGC 6 cut(s) 843, 884, 1038, 1107, 1344, 1393
BstDEI CTNAG 4 cut(s) 94, 186, 688, 874
BstENI CCTNNNNNAGG 1 cut(s) 687
BstF5I GGATG 3 cut(s) 604, 623, 1225
BstFNI CGCG 1 cut(s) 147
BstKTI GATC 6 cut(s) 193, 658, 788, 862, 901, 1171
BstMBI GATC 6 cut(s) 190, 655, 785, 859, 898, 1168
BstMWI GCNNNNNNNGC 5 cut(s) 404, 914, 1037, 1106, 1292
BstNI CCWGG 5 cut(s) 46, 339, 913, 1338, 1381
BstNSI RCATGY 2 cut(s) 749, 1295
BstSCI CCNGG 5 cut(s) 44, 337, 911, 1336, 1379
BstSFI CTRYAG 2 cut(s) 1211, 1281
BstUI CGCG 1 cut(s) 147
BstV1I GCAGC 8 cut(s) 169, 666, 920, 1033, 1087, 1132, 1237, 1358
BstV2I GAAGAC 2 cut(s) 25, 75
BstX2I RGATCY 2 cut(s) 785, 898
BstYI RGATCY 2 cut(s) 785, 898
Bsu36I CCTNAGG 1 cut(s) 688
BsuI GTATCC 1 cut(s) 977
BsuRI GGCC 2 cut(s) 337, 1391
BtsCI GGATG 3 cut(s) 604, 623, 1225
BtsI GCAGTG 2 cut(s) 402, 776
BtsIMutI CAGTG 3 cut(s) 402, 596, 776
BveI ACCTGC 1 cut(s) 288
Cac8I GCNNGC 6 cut(s) 843, 884, 1038, 1107, 1344, 1393
Cfr10I RCCGGY 3 cut(s) 79, 668, 841
Cfr13I GGNCC 3 cut(s) 77, 208, 1389
CseI GACGC 1 cut(s) 943
Csp6I GTAC 1 cut(s) 696
CspAI ACCGGT 1 cut(s) 668
CviQI GTAC 1 cut(s) 696
DdeI CTNAG 4 cut(s) 94, 186, 688, 874
DpnI GATC 6 cut(s) 192, 657, 787, 861, 900, 1170
DpnII GATC 6 cut(s) 190, 655, 785, 859, 898, 1168
DraI TTTAAA 1 cut(s) 511
DraIII CACNNNGTG 1 cut(s) 1276
Eco47I GGWCC 2 cut(s) 77, 208
Eco57I CTGAAG 2 cut(s) 688, 1110
Eco81I CCTNAGG 1 cut(s) 688
EcoNI CCTNNNNNAGG 1 cut(s) 687
EcoRII CCWGG 5 cut(s) 44, 337, 911, 1336, 1379
EcoT22I ATGCAT 1 cut(s) 717
FalI AAGNNNNNCTT 8 cut(s) 404, 436, 418, 450, 667, 699, 871, 903
FaqI GGGAC 1 cut(s) 1366
FauI CCCGC 1 cut(s) 310
FauNDI CATATG 1 cut(s) 1120
FbaI TGATCA 1 cut(s) 1168
Fnu4HI GCNGC 8 cut(s) 183, 680, 909, 1022, 1076, 1146, 1251, 1347
FokI GGATG 3 cut(s) 591, 610, 1232
Fsp4HI GCNGC 8 cut(s) 183, 680, 909, 1022, 1076, 1146, 1251, 1347
FspBI CTAG 1 cut(s) 975
GluI GCNGC 8 cut(s) 183, 680, 909, 1022, 1076, 1146, 1251, 1347
GsaI CCCAGC 1 cut(s) 1105
HaeIII GGCC 2 cut(s) 337, 1391
HapII CCGG 3 cut(s) 80, 669, 842
HgaI GACGC 1 cut(s) 943
HinfI GANTC 2 cut(s) 465, 759
HpaII CCGG 3 cut(s) 80, 669, 842
HphI GGTGA 2 cut(s) 472, 912
Hpy166II GTNNAC 3 cut(s) 88, 827, 1323
Hpy188I TCNGA 4 cut(s) 666, 1050, 1330, 1362
Hpy188III TCNNGA 2 cut(s) 221, 524
Hpy8I GTNNAC 3 cut(s) 88, 827, 1323
HpyAV CCTTC 5 cut(s) 646, 693, 850, 889, 897
HpyCH4III ACNGT 5 cut(s) 287, 600, 641, 1199, 1357
HpyCH4IV ACGT 2 cut(s) 514, 1319
HpyF10VI GCNNNNNNNGC 5 cut(s) 404, 914, 1037, 1106, 1292
HpyF3I CTNAG 4 cut(s) 94, 186, 688, 874
HpySE526I ACGT 2 cut(s) 514, 1319
KroI GCCGGC 1 cut(s) 841
KroNI GCCGGC 1 cut(s) 843
Ksp22I TGATCA 1 cut(s) 1168
Kzo9I GATC 6 cut(s) 190, 655, 785, 859, 898, 1168
LmnI GCTCC 3 cut(s) 121, 931, 1300
Lsp1109I GCAGC 8 cut(s) 169, 666, 920, 1033, 1087, 1132, 1237, 1358
LweI GCATC 4 cut(s) 88, 169, 1087, 1353
MaeI CTAG 1 cut(s) 975
MaeII ACGT 2 cut(s) 514, 1319
MaeIII GTNAC 2 cut(s) 1093, 1193
MalI GATC 6 cut(s) 192, 657, 787, 861, 900, 1170
MboI GATC 6 cut(s) 190, 655, 785, 859, 898, 1168
MboII GAAGA 7 cut(s) 25, 80, 144, 236, 270, 321, 340
MflI RGATCY 2 cut(s) 785, 898
MluCI AATT 6 cut(s) 227, 471, 561, 659, 1067, 1375
MlyI GAGTC 1 cut(s) 768
MmeI TCCRAC 1 cut(s) 909
MnlI CCTC 9 cut(s) 133, 181, 497, 533, 697, 750, 883, 945, 1135
Mph1103I ATGCAT 1 cut(s) 717
MroNI GCCGGC 1 cut(s) 841
MseI TTAA 2 cut(s) 510, 555
MspA1I CMGCKG 1 cut(s) 305
MspI CCGG 3 cut(s) 80, 669, 842
MspR9I CCNGG 5 cut(s) 46, 339, 913, 1338, 1381
Mva1269I GAATGC 4 cut(s) 278, 488, 715, 1252
MvaI CCWGG 5 cut(s) 46, 339, 913, 1338, 1381
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 5 cut(s) 404, 914, 1037, 1106, 1292
NaeI GCCGGC 1 cut(s) 843
NdeI CATATG 1 cut(s) 1120
NdeII GATC 6 cut(s) 190, 655, 785, 859, 898, 1168
NgoMIV GCCGGC 1 cut(s) 841
NlaIV GGNNCC 5 cut(s) 544, 787, 900, 933, 1390
NsiI ATGCAT 1 cut(s) 717
NspI RCATGY 2 cut(s) 749, 1295
PciI ACATGT 1 cut(s) 745
PctI GAATGC 4 cut(s) 278, 488, 715, 1252
PdiI GCCGGC 1 cut(s) 843
PfeI GAWTC 1 cut(s) 465
PfoI TCCNGGA 1 cut(s) 1379
PinAI ACCGGT 1 cut(s) 668
PkrI GCNGC 8 cut(s) 184, 681, 910, 1023, 1077, 1147, 1252, 1348
PleI GAGTC 1 cut(s) 767
PpsI GAGTC 1 cut(s) 767
PscI ACATGT 1 cut(s) 745
PsiI TTATAA 1 cut(s) 366
Psp6I CCWGG 5 cut(s) 44, 337, 911, 1336, 1379
PspFI CCCAGC 1 cut(s) 1101
PspGI CCWGG 5 cut(s) 44, 337, 911, 1336, 1379
PspN4I GGNNCC 5 cut(s) 544, 787, 900, 933, 1390
PspPI GGNCC 3 cut(s) 77, 208, 1389
PsrI GAACNNNNNNTAC 2 cut(s) 986, 1018
PstI CTGCAG 1 cut(s) 1215
PsuI RGATCY 2 cut(s) 785, 898
RsaI GTAC 1 cut(s) 697
RsaNI GTAC 1 cut(s) 696
SaqAI TTAA 2 cut(s) 510, 555
SatI GCNGC 8 cut(s) 183, 680, 909, 1022, 1076, 1146, 1251, 1347
Sau3AI GATC 6 cut(s) 190, 655, 785, 859, 898, 1168
Sau96I GGNCC 3 cut(s) 77, 208, 1389
SchI GAGTC 1 cut(s) 768
ScrFI CCNGG 5 cut(s) 46, 339, 913, 1338, 1381
SfaNI GCATC 4 cut(s) 88, 169, 1087, 1353
SfcI CTRYAG 2 cut(s) 1211, 1281
SinI GGWCC 2 cut(s) 77, 208
Sse9I AATT 6 cut(s) 227, 471, 561, 659, 1067, 1375
SsiI CCGC 2 cut(s) 145, 303
SspI AATATT 1 cut(s) 21
SspMI CTAG 1 cut(s) 975
StyD4I CCNGG 5 cut(s) 44, 337, 911, 1336, 1379
TaaI ACNGT 5 cut(s) 287, 600, 641, 1199, 1357
TaiI ACGT 2 cut(s) 517, 1322
TasI AATT 6 cut(s) 227, 471, 561, 659, 1067, 1375
TatI WGTACW 1 cut(s) 695
TfiI GAWTC 1 cut(s) 465
Tru1I TTAA 2 cut(s) 510, 555
Tru9I TTAA 2 cut(s) 510, 555
TscAI CASTG 3 cut(s) 409, 603, 776
TseI GCWGC 8 cut(s) 182, 679, 908, 1021, 1075, 1145, 1250, 1346
TspGWI ACGGA 1 cut(s) 1232
TspRI CASTG 3 cut(s) 409, 603, 776
VpaK11BI GGWCC 2 cut(s) 77, 208
XagI CCTNNNNNAGG 1 cut(s) 687
XapI RAATTY 4 cut(s) 227, 471, 1067, 1375
XceI RCATGY 2 cut(s) 749, 1295
XcmI CCANNNNNNNNNTGG 2 cut(s) 796, 909
XspI CTAG 1 cut(s) 975
Zsp2I ATGCAT 1 cut(s) 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.