pycom13g11380

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0000847
Physical Location & Seq
Forward (+)
7816830 .. 7819287
2458 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g11380.4

Sequence Viewer

Length: 1284 bp
ATGCAGATAAGTGCATCTTCAGTGCCTACGCCGGCTCCTGTGCCTGACAGCGGCCCTTCCACTTCCAATGTTGCTCATGCAACTTCGTCTAGCCATGCTAATCCTACTGGTAGAAAAAAGGTTCGGCCAACAAAGAATGAGAAGGATAGTGGGCACTCTCATTCTGGTTTTATATTTATGTGTAGTGGGAAAACGAAACCCGAGTGTTATCGCTATCGTGTTTTTGGGCTGCCGTTAGCAAAGGTAGATGTTGTGAAGAAAATTAGACCGGGTGCAAAGCTATTTCTATACGATTTTGACTTGAAGCTCCTTTATGGCACGTATAAGGCAACTTCAAGTGGAGGATTGGATTTGGAACCAATTGCGTTTAAGGGGAAATTCCCTGCACAGGTCAGATTTAAGATTTTCAAGGATTGTTTACCTCTTCCTGAAAATGCTTTTAAAGCAGCTATCAGAGACAACTACCAGGGAGGTTCTAAGTTTAAACAAGAGCTTAATAGCAAACAGGTGCATGCTTTAATCTCATTGTTCTGCCCTCTCACCGAGTTAGTGCCTACTACTGCTCCCCCTCTCCGAGATGTGGCAAGACCAGGCTCATTTCCATCTCCTATTACAGAAAAAGGATTTCATCCAACAGCAAGGCTGTCCCATCAAGAGGGCTCCTATTCATATGGTGTGCAACGTTCTCATGCATTGCCGCTTGATACGCAATCCAGGCAGGCAATCCCGTATCCACTGTATGATCAATATGAGGTCAAAACACATGTGGAATGTTTGCGGCGTCACATGGAGCCTCAACATGTTCAGCATGCTTCTCTTCCACATCATGCCGATTCATATTATTTAGCAGCAGCACATGAGCCGTACTTGCCTGAAAACCTTTCCCATCATGACGGATATAGAAGGCATAGGGTGACACTTGCAGAGGAGATGGTTCCCAGGGATCAAGTTGTTTTGTATGGAAGCGAGTATGACATGCCCCAGTTGTTGAAGGAGAGAGAAAGAGATATTGCTCTGCGTTCCAATTATGTGGCTGAATCTTATAGTCGACAGTTGCCCACCGCTGCAGCCGCACATGTTATGCTGCAGCCACATTCTTTAGCACCATCTTATGAACGAACATTAACGTACCAACGGTACTATCCAGCAACAACCCATCAGGATCAGAGCTTGGTGTATGCTGATCCTCTTCGAAGACCATTGCATGGTACCTCTTACTCAGCTGAGGCAGGTGTTCCAATCTCTACACATTATTCGTACGCTAGAGCTCCCCGTTACCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

428

Amino Acids

48.04

Weight (kDa)

9.31

Isoelectric Point (pI)

47.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1220
Acc36I ACCTGC 1 cut(s) 1220
Acc65I GGTACC 1 cut(s) 1208
AccB1I GGYRCC 1 cut(s) 1208
AccB7I CCANNNNNTGG 1 cut(s) 1205
AccI GTMKAC 1 cut(s) 1048
AciI CCGC 5 cut(s) 51, 698, 778, 1062, 1071
AclI AACGTT 1 cut(s) 682
AclWI GGATC 3 cut(s) 951, 1170, 1178
AcoI YGGCCR 1 cut(s) 125
AcsI RAATTY 1 cut(s) 377
AcyI GRCGYC 1 cut(s) 781
AfaI GTAC 5 cut(s) 866, 1130, 1139, 1210, 1259
AfiI CCNNNNNNNGG 5 cut(s) 50, 388, 580, 655, 1205
AflIII ACRYGT 3 cut(s) 763, 799, 1075
AgsI TTSAA 4 cut(s) 304, 336, 409, 991
AjnI CCWGG 4 cut(s) 465, 589, 713, 938
AluBI AGCT 7 cut(s) 280, 307, 449, 493, 1170, 1223, 1268
AluI AGCT 7 cut(s) 280, 307, 449, 493, 1170, 1223, 1268
Alw21I GWGCWC 1 cut(s) 1270
Alw26I GTCTC 1 cut(s) 450
AlwI GGATC 3 cut(s) 951, 1170, 1178
Ama87I CYCGRG 1 cut(s) 200
AoxI GGCC 2 cut(s) 52, 125
ApeKI GCWGC 8 cut(s) 229, 446, 848, 851, 1064, 1067, 1084, 1087
ApoI RAATTY 1 cut(s) 377
Asp718I GGTACC 1 cut(s) 1208
AspS9I GGNCC 1 cut(s) 53
AsuC2I CCSGG 1 cut(s) 270
AsuHPI GGTGA 2 cut(s) 532, 925
AsuII TTCGAA 1 cut(s) 1192
AvaI CYCGRG 1 cut(s) 200
BaeGI GKGCMC 1 cut(s) 156
BanI GGYRCC 1 cut(s) 1208
BanII GRGCYC 2 cut(s) 662, 1270
BbsI GAAGAC 1 cut(s) 1201
Bbv12I GWGCWC 1 cut(s) 1270
BbvCI CCTCAGC 1 cut(s) 1224
BbvI GCAGC 8 cut(s) 216, 458, 860, 863, 1051, 1071, 1079, 1099
BccI CCATC 6 cut(s) 610, 657, 894, 925, 1114, 1164
BceAI ACGGC 2 cut(s) 217, 847
BciT130I CCWGG 4 cut(s) 467, 591, 715, 940
BciVI GTATCC 1 cut(s) 741
BclI TGATCA 1 cut(s) 742
BcnI CCSGG 1 cut(s) 270
BcoDI GTCTC 1 cut(s) 450
BfaI CTAG 2 cut(s) 90, 1263
BfmI CTRYAG 2 cut(s) 1065, 1085
BfuAI ACCTGC 1 cut(s) 1220
BfuI GTATCC 1 cut(s) 741
Bme1390I CCNGG 5 cut(s) 270, 467, 591, 715, 940
BmeT110I CYCGRG 1 cut(s) 200
BmgT120I GGNCC 1 cut(s) 53
BmiI GGNNCC 6 cut(s) 36, 357, 661, 792, 936, 1210
BmrFI CCNGG 5 cut(s) 270, 467, 591, 715, 940
BmrI ACTGGG 1 cut(s) 976
BmsI GCATC 1 cut(s) 23
BmuI ACTGGG 1 cut(s) 976
BpiI GAAGAC 1 cut(s) 1201
Bpu10I CCTNAGC 1 cut(s) 1224
Bpu14I TTCGAA 1 cut(s) 1192
BpuMI CCSGG 1 cut(s) 270
BsaAI YACGTR 1 cut(s) 321
BsaHI GRCGYC 1 cut(s) 781
BsaJI CCNNGG 3 cut(s) 466, 938, 939
BsaXI ACNNNNNCTCC 4 cut(s) 19, 49, 547, 577
Bsc4I CCNNNNNNNGG 5 cut(s) 50, 388, 580, 655, 1205
Bse118I RCCGGY 1 cut(s) 31
Bse1I ACTGG 2 cut(s) 112, 982
Bse3DI GCAATG 2 cut(s) 692, 1199
BseBI CCWGG 4 cut(s) 467, 591, 715, 940
BseDI CCNNGG 3 cut(s) 466, 938, 939
BseGI GGATG 1 cut(s) 628
BseLI CCNNNNNNNGG 5 cut(s) 50, 388, 580, 655, 1205
BseMI GCAATG 2 cut(s) 692, 1199
BseMII CTCAG 2 cut(s) 1215, 1233
BseNI ACTGG 2 cut(s) 112, 982
BseRI GAGGAG 1 cut(s) 941
BseSI GKGCMC 1 cut(s) 156
BseXI GCAGC 8 cut(s) 216, 458, 860, 863, 1051, 1071, 1079, 1099
BsgI GTGCAG 1 cut(s) 369
BshFI GGCC 2 cut(s) 54, 127
BshNI GGYRCC 1 cut(s) 1208
BsiHKAI GWGCWC 1 cut(s) 1270
BsiHKCI CYCGRG 1 cut(s) 200
BsiSI CCGG 2 cut(s) 32, 269
BsiWI CGTACG 1 cut(s) 1257
BslFI GGGAC 1 cut(s) 631
BslI CCNNNNNNNGG 5 cut(s) 50, 388, 580, 655, 1205
BsmAI GTCTC 1 cut(s) 450
BsmFI GGGAC 1 cut(s) 631
BsnI GGCC 2 cut(s) 54, 127
BsoBI CYCGRG 1 cut(s) 200
Bsp119I TTCGAA 1 cut(s) 1192
Bsp1286I GDGCHC 3 cut(s) 156, 662, 1270
Bsp143I GATC 4 cut(s) 742, 943, 1162, 1183
BspACI CCGC 5 cut(s) 51, 698, 778, 1062, 1071
BspANI GGCC 2 cut(s) 54, 127
BspCNI CTCAG 2 cut(s) 1216, 1232
BspHI TCATGA 1 cut(s) 889
BspLI GGNNCC 6 cut(s) 36, 357, 661, 792, 936, 1210
BspMAI CTGCAG 2 cut(s) 1069, 1089
BspMI ACCTGC 1 cut(s) 1220
BspPI GGATC 3 cut(s) 951, 1170, 1178
BspT104I TTCGAA 1 cut(s) 1192
BspT107I GGYRCC 1 cut(s) 1208
BsrDI GCAATG 2 cut(s) 692, 1199
BsrFI RCCGGY 1 cut(s) 31
BsrI ACTGG 2 cut(s) 112, 982
BssAI RCCGGY 1 cut(s) 31
BssECI CCNNGG 3 cut(s) 466, 938, 939
BssMI GATC 4 cut(s) 742, 943, 1162, 1183
BssNI GRCGYC 1 cut(s) 781
Bst2UI CCWGG 4 cut(s) 467, 591, 715, 940
Bst4CI ACNGT 3 cut(s) 738, 1053, 1137
Bst6I CTCTTC 3 cut(s) 429, 822, 1194
BstACI GRCGYC 1 cut(s) 781
BstBAI YACGTR 1 cut(s) 321
BstBI TTCGAA 1 cut(s) 1192
BstC8I GCNNGC 4 cut(s) 33, 513, 720, 810
BstDEI CTNAG 3 cut(s) 477, 1219, 1224
BstF5I GGATG 1 cut(s) 628
BstKTI GATC 4 cut(s) 745, 946, 1165, 1186
BstMAI GTCTC 1 cut(s) 450
BstMBI GATC 4 cut(s) 742, 943, 1162, 1183
BstMWI GCNNNNNNNGC 5 cut(s) 443, 706, 715, 868, 1070
BstNI CCWGG 4 cut(s) 467, 591, 715, 940
BstNSI RCATGY 6 cut(s) 515, 767, 803, 812, 979, 1079
BstSCI CCNGG 5 cut(s) 268, 465, 589, 713, 938
BstSFI CTRYAG 2 cut(s) 1065, 1085
BstSLI GKGCMC 1 cut(s) 156
BstV1I GCAGC 8 cut(s) 216, 458, 860, 863, 1051, 1071, 1079, 1099
BstV2I GAAGAC 1 cut(s) 1201
BstXI CCANNNNNNTGG 1 cut(s) 1030
BsuI GTATCC 1 cut(s) 741
BsuRI GGCC 2 cut(s) 54, 127
BtsCI GGATG 1 cut(s) 628
BtsIMutI CAGTG 2 cut(s) 27, 734
BveI ACCTGC 1 cut(s) 1220
Cac8I GCNNGC 4 cut(s) 33, 513, 720, 810
CciI TCATGA 1 cut(s) 889
Cfr10I RCCGGY 1 cut(s) 31
Cfr13I GGNCC 1 cut(s) 53
CseI GACGC 1 cut(s) 770
Csp6I GTAC 5 cut(s) 865, 1129, 1138, 1209, 1258
CviQI GTAC 5 cut(s) 865, 1129, 1138, 1209, 1258
DdeI CTNAG 3 cut(s) 477, 1219, 1224
DpnI GATC 4 cut(s) 744, 945, 1164, 1185
DpnII GATC 4 cut(s) 742, 943, 1162, 1183
DraI TTTAAA 2 cut(s) 442, 484
EaeI YGGCCR 1 cut(s) 125
Eam1104I CTCTTC 3 cut(s) 429, 822, 1194
EarI CTCTTC 3 cut(s) 429, 822, 1194
Ecl136II GAGCTC 1 cut(s) 1268
Eco24I GRGCYC 2 cut(s) 662, 1270
Eco53kI GAGCTC 1 cut(s) 1268
Eco88I CYCGRG 1 cut(s) 200
EcoICRI GAGCTC 1 cut(s) 1268
EcoRII CCWGG 4 cut(s) 465, 589, 713, 938
EcoT22I ATGCAT 1 cut(s) 694
EcoT38I GRGCYC 2 cut(s) 662, 1270
FalI AAGNNNNNCTT 1 cut(s) 33
FaqI GGGAC 1 cut(s) 631
FauNDI CATATG 1 cut(s) 670
FbaI TGATCA 1 cut(s) 742
FblI GTMKAC 1 cut(s) 1048
FokI GGATG 1 cut(s) 615
FriOI GRGCYC 2 cut(s) 662, 1270
FspBI CTAG 2 cut(s) 90, 1263
HaeIII GGCC 2 cut(s) 54, 127
HapII CCGG 2 cut(s) 32, 269
HgaI GACGC 1 cut(s) 770
Hin1I GRCGYC 1 cut(s) 781
HincII GTYRAC 1 cut(s) 1049
HindII GTYRAC 1 cut(s) 1049
HinfI GANTC 2 cut(s) 833, 1037
HpaII CCGG 2 cut(s) 32, 269
HphI GGTGA 2 cut(s) 532, 925
Hpy166II GTNNAC 2 cut(s) 419, 1049
Hpy188I TCNGA 4 cut(s) 395, 455, 575, 1167
Hpy188III TCNNGA 4 cut(s) 428, 653, 890, 1160
Hpy8I GTNNAC 2 cut(s) 419, 1049
HpyAV CCTTC 4 cut(s) 66, 136, 897, 985
HpyCH4III ACNGT 3 cut(s) 738, 1053, 1137
HpyCH4IV ACGT 3 cut(s) 320, 682, 1127
HpyF10VI GCNNNNNNNGC 5 cut(s) 443, 706, 715, 868, 1070
HpyF3I CTNAG 3 cut(s) 477, 1219, 1224
HpySE526I ACGT 3 cut(s) 320, 682, 1127
Hsp92I GRCGYC 1 cut(s) 781
KpnI GGTACC 1 cut(s) 1212
KroI GCCGGC 1 cut(s) 31
KroNI GCCGGC 1 cut(s) 33
Ksp22I TGATCA 1 cut(s) 742
Kzo9I GATC 4 cut(s) 742, 943, 1162, 1183
LmnI GCTCC 6 cut(s) 40, 312, 568, 665, 790, 1273
Lsp1109I GCAGC 8 cut(s) 216, 458, 860, 863, 1051, 1071, 1079, 1099
LweI GCATC 1 cut(s) 23
MaeI CTAG 2 cut(s) 90, 1263
MaeII ACGT 3 cut(s) 320, 682, 1127
MaeIII GTNAC 3 cut(s) 782, 913, 1274
MalI GATC 4 cut(s) 744, 945, 1164, 1185
MboI GATC 4 cut(s) 742, 943, 1162, 1183
MboII GAAGA 6 cut(s) 9, 268, 416, 809, 1181, 1206
MfeI CAATTG 1 cut(s) 360
MhlI GDGCHC 3 cut(s) 156, 662, 1270
MluCI AATT 4 cut(s) 261, 360, 377, 1024
MmeI TCCRAC 1 cut(s) 656
Mph1103I ATGCAT 1 cut(s) 694
MroNI GCCGGC 1 cut(s) 31
MseI TTAA 7 cut(s) 369, 399, 441, 483, 495, 518, 1124
MspA1I CMGCKG 3 cut(s) 51, 1064, 1223
MspI CCGG 2 cut(s) 32, 269
MspR9I CCNGG 5 cut(s) 270, 467, 591, 715, 940
MssI GTTTAAAC 1 cut(s) 484
MunI CAATTG 1 cut(s) 360
MvaI CCWGG 4 cut(s) 467, 591, 715, 940
MwoI GCNNNNNNNGC 5 cut(s) 443, 706, 715, 868, 1070
NaeI GCCGGC 1 cut(s) 33
NciI CCSGG 1 cut(s) 270
NdeI CATATG 1 cut(s) 670
NdeII GATC 4 cut(s) 742, 943, 1162, 1183
NgoMIV GCCGGC 1 cut(s) 31
NlaIV GGNNCC 6 cut(s) 36, 357, 661, 792, 936, 1210
NmuCI GTSAC 2 cut(s) 782, 913
NsiI ATGCAT 1 cut(s) 694
NspI RCATGY 6 cut(s) 515, 767, 803, 812, 979, 1079
NspV TTCGAA 1 cut(s) 1192
PaeI GCATGC 2 cut(s) 515, 812
PagI TCATGA 1 cut(s) 889
PaqCI CACCTGC 1 cut(s) 1220
PasI CCCWGGG 1 cut(s) 939
PciI ACATGT 3 cut(s) 763, 799, 1075
PdiI GCCGGC 1 cut(s) 33
PfeI GAWTC 2 cut(s) 833, 1037
Pfl23II CGTACG 1 cut(s) 1257
PflMI CCANNNNNTGG 1 cut(s) 1205
PmeI GTTTAAAC 1 cut(s) 484
Ppu21I YACGTR 1 cut(s) 321
PscI ACATGT 3 cut(s) 763, 799, 1075
Psp124BI GAGCTC 1 cut(s) 1270
Psp1406I AACGTT 1 cut(s) 682
Psp6I CCWGG 4 cut(s) 465, 589, 713, 938
PspGI CCWGG 4 cut(s) 465, 589, 713, 938
PspLI CGTACG 1 cut(s) 1257
PspN4I GGNNCC 6 cut(s) 36, 357, 661, 792, 936, 1210
PspPI GGNCC 1 cut(s) 53
PstI CTGCAG 2 cut(s) 1069, 1089
PvuII CAGCTG 1 cut(s) 1223
RsaI GTAC 5 cut(s) 866, 1130, 1139, 1210, 1259
RsaNI GTAC 5 cut(s) 865, 1129, 1138, 1209, 1258
SacI GAGCTC 1 cut(s) 1270
SalI GTCGAC 1 cut(s) 1047
SaqAI TTAA 7 cut(s) 369, 399, 441, 483, 495, 518, 1124
Sau3AI GATC 4 cut(s) 742, 943, 1162, 1183
Sau96I GGNCC 1 cut(s) 53
ScrFI CCNGG 5 cut(s) 270, 467, 591, 715, 940
SduI GDGCHC 3 cut(s) 156, 662, 1270
SfaNI GCATC 1 cut(s) 23
SfcI CTRYAG 2 cut(s) 1065, 1085
SfuI TTCGAA 1 cut(s) 1192
SphI GCATGC 2 cut(s) 515, 812
Sse9I AATT 4 cut(s) 261, 360, 377, 1024
SsiI CCGC 5 cut(s) 51, 698, 778, 1062, 1071
SspMI CTAG 2 cut(s) 90, 1263
SstI GAGCTC 1 cut(s) 1270
StyD4I CCNGG 5 cut(s) 268, 465, 589, 713, 938
TaaI ACNGT 3 cut(s) 738, 1053, 1137
TaiI ACGT 3 cut(s) 323, 685, 1130
TaqI TCGA 2 cut(s) 1048, 1192
TasI AATT 4 cut(s) 261, 360, 377, 1024
TauI GCSGC 4 cut(s) 54, 700, 781, 1073
TfiI GAWTC 2 cut(s) 833, 1037
Tru1I TTAA 7 cut(s) 369, 399, 441, 483, 495, 518, 1124
Tru9I TTAA 7 cut(s) 369, 399, 441, 483, 495, 518, 1124
TscAI CASTG 2 cut(s) 27, 741
TseFI GTSAC 2 cut(s) 782, 913
TseI GCWGC 8 cut(s) 229, 446, 848, 851, 1064, 1067, 1084, 1087
Tsp45I GTSAC 2 cut(s) 782, 913
TspDTI ATGAA 4 cut(s) 617, 657, 825, 1128
TspGWI ACGGA 1 cut(s) 909
TspRI CASTG 2 cut(s) 27, 741
Van91I CCANNNNNTGG 1 cut(s) 1205
XapI RAATTY 1 cut(s) 377
XceI RCATGY 6 cut(s) 515, 767, 803, 812, 979, 1079
XmiI GTMKAC 1 cut(s) 1048
XspI CTAG 2 cut(s) 90, 1263
Zsp2I ATGCAT 1 cut(s) 694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.