Rh4DG289800

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000847
Physical Location & Seq
Reverse (-)
51092387 .. 51096823
4437 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG289800.1

Sequence Viewer

Length: 1386 bp
ATGGGAAAAAGACGAGGCAAGCGTCTAAGCAAAAGAGGCCTAACTAGAGATCATGATGGTAGGATTAGGAGGATAGACAAGAGTTTTACAAAGGAAAAGAAGAAATCTAAGAAGATAACCGAATCTTCCTTGCCTCAGACTGCAGCTGAGCTTAGTGGTAGTACTAGTGCCAATTCTGCTTCCAATAAAGCAACTTCTTCCAGTCATGCTAATCAACCTACTAGAGAAAGGCTTCGATCAAAAAACAAAGGCAAGGATAGTGGGCAATCACATTCTGGTTTTATCTTTATGTGTAATGGAAAGACTAAACCCGAATGTTATCAGTATCGCGTTTTTGGGCTACCAATGGCAAAAGTAGAAGTTGTGAAGACAATCAAACAGGGTACACATCTATTTTTATATGACTTTGACTTGAAGCTCCTTTATGGCACATACAGCGCAACTTCGGATGGAGCATTAGATTTGGAGCCAATTGCGTTCAAGGGAAAATTTCCTGCACAGGTCAGGTTTAAGATTTCCAAGGATTGTTTACCACTTCACGAAAGTTCTTTTAAAACAGCCATAAGAGACAATTACCAGGGCGGTGGTAAGTTTAAACAAGAGCTGAATAACAAACAGGTGCGTGCTCTAATCTCATTGTTCCGCCCAATCACTGCATCAATGCCTACTTCTGCTGCCCATCACCGAGATGTGGCAAGAACAGGCTCATATCCATCTCCTATTAGGGAAGAAGGATTTCATCCAACAGCAAGGCCGTCTCTTCAAGAGGGCTTATATTTATCTAGCGTACAACATGCTCATGCATTGCCACTTCTTAATACGCAATCCATACAGGTTGTTCCCTATCCACGTTTTAAAAAATATGAAGCAGAAAGATGTGTGGCACGTGTTCAGCCTCCCATAGAGCCTCGACATGTTGTTCAGCATCAGCCAGATTCATATTATCTAGCAGCGGCCCATGAGCCATACGTGCCTGAAAAACCTTTCTTGTCCTCCCATGATGCATATAGAAGGCATGGGTTCACACTTCCACGGGAGATGGTTCCTAGGGATCAAGATGTTTCATATGGAAGTGTGTATCGCTTGTCACAGTTGCTGAAGGAGAGAGAAAGAGATATTGTTTCCCATTCCGATTATATTGCTGAAACCTATAGTCGACACTTGCCAACTCATCCAGCCTCACAGTTTAGGTTGCAGTCACATTCTTCGGCATCATCTTATGAGTTACCATCAACTTACCAACCATATGATCAAGCAACGACCCATCAGGATCAGAACCCGGTGGATCGTGATCCTCGTCAGAGACCATTGCATAGGAGCTCAAATGAGGCAAGTTTGCCAATCTGTTCTCACTTGTCTTCCACTGGAGCTGCCCGCTACTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

461

Amino Acids

52.22

Weight (kDa)

9.77

Isoelectric Point (pI)

44.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 93 - 214 2e-49 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1156
AccII CGCG 1 cut(s) 330
AciI CCGC 4 cut(s) 582, 643, 953, 1375
AclWI GGATC 4 cut(s) 1059, 1278, 1286, 1293
AcsI RAATTY 1 cut(s) 488
AcuI CTGAAG 1 cut(s) 1118
AcvI CACGTG 1 cut(s) 887
AfaI GTAC 3 cut(s) 163, 385, 789
AfiI CCNNNNNNNGG 1 cut(s) 691
AflIII ACRYGT 2 cut(s) 886, 913
AgsI TTSAA 3 cut(s) 415, 481, 764
AhlI ACTAGT 1 cut(s) 164
AjnI CCWGG 1 cut(s) 576
AluBI AGCT 6 cut(s) 146, 151, 418, 604, 1320, 1370
AluI AGCT 6 cut(s) 146, 151, 418, 604, 1320, 1370
Alw21I GWGCWC 2 cut(s) 628, 1322
Alw26I GTCTC 3 cut(s) 561, 762, 1297
AlwI GGATC 4 cut(s) 1059, 1278, 1286, 1293
AlwNI CAGNNNCTG 1 cut(s) 1096
AoxI GGCC 3 cut(s) 37, 752, 954
ApeKI GCWGC 4 cut(s) 143, 674, 950, 1370
ApoI RAATTY 1 cut(s) 488
Asp700I GAANNNNTTC 2 cut(s) 231, 735
AspA2I CCTAGG 1 cut(s) 1046
AspLEI GCGC 1 cut(s) 440
AspS9I GGNCC 1 cut(s) 955
AsuC2I CCSGG 1 cut(s) 1280
AsuHPI GGTGA 1 cut(s) 674
AvrII CCTAGG 1 cut(s) 1046
BanII GRGCYC 1 cut(s) 1322
BbrPI CACGTG 1 cut(s) 887
BbsI GAAGAC 2 cut(s) 374, 1350
Bbv12I GWGCWC 2 cut(s) 628, 1322
BbvI GCAGC 4 cut(s) 155, 661, 962, 1357
BccI CCATC 7 cut(s) 50, 443, 687, 721, 1033, 1237, 1272
BceAI ACGGC 1 cut(s) 739
BcgI CGANNNNNNTGC 2 cut(s) 1121, 1155
BciT130I CCWGG 1 cut(s) 578
BclI TGATCA 1 cut(s) 1249
BcnI CCSGG 1 cut(s) 1280
BcoDI GTCTC 3 cut(s) 561, 762, 1297
BcuI ACTAGT 1 cut(s) 164
BfaI CTAG 6 cut(s) 45, 165, 222, 783, 947, 1047
BfmI CTRYAG 2 cut(s) 141, 1150
BisI GCNGC 5 cut(s) 144, 675, 951, 954, 1371
BlnI CCTAGG 1 cut(s) 1046
BlpI GCTNAGC 1 cut(s) 147
BlsI GCNGC 5 cut(s) 145, 676, 952, 955, 1372
BmcAI AGTACT 1 cut(s) 163
Bme1390I CCNGG 2 cut(s) 578, 1280
BmgT120I GGNCC 1 cut(s) 955
BmiI GGNNCC 2 cut(s) 468, 1044
BmrFI CCNGG 2 cut(s) 578, 1280
BmsI GCATC 4 cut(s) 665, 934, 991, 1220
BpiI GAAGAC 2 cut(s) 374, 1350
BpmI CTGGAG 1 cut(s) 1386
Bpu1102I GCTNAGC 1 cut(s) 147
BpuMI CCSGG 1 cut(s) 1280
BsaAI YACGTR 2 cut(s) 887, 970
BsaBI GATNNNNATC 1 cut(s) 1290
BsaI GGTCTC 1 cut(s) 1297
BsaJI CCNNGG 4 cut(s) 519, 577, 1031, 1046
Bsc4I CCNNNNNNNGG 1 cut(s) 691
Bse1I ACTGG 2 cut(s) 201, 1369
Bse3DI GCAATG 2 cut(s) 803, 1307
Bse8I GATNNNNATC 1 cut(s) 1290
BseBI CCWGG 1 cut(s) 578
BseDI CCNNGG 4 cut(s) 519, 577, 1031, 1046
BseGI GGATG 3 cut(s) 454, 739, 1171
BseJI GATNNNNATC 1 cut(s) 1290
BseLI CCNNNNNNNGG 1 cut(s) 691
BseMI GCAATG 2 cut(s) 803, 1307
BseMII CTCAG 2 cut(s) 138, 149
BseNI ACTGG 2 cut(s) 201, 1369
BseXI GCAGC 4 cut(s) 155, 661, 962, 1357
BsgI GTGCAG 1 cut(s) 480
Bsh1236I CGCG 1 cut(s) 330
BshFI GGCC 3 cut(s) 39, 754, 956
BsiHKAI GWGCWC 2 cut(s) 628, 1322
BsiSI CCGG 1 cut(s) 1280
BslI CCNNNNNNNGG 1 cut(s) 691
BsmAI GTCTC 3 cut(s) 561, 762, 1297
BsmBI CGTCTC 1 cut(s) 762
BsnI GGCC 3 cut(s) 39, 754, 956
Bso31I GGTCTC 1 cut(s) 1297
Bsp1286I GDGCHC 2 cut(s) 628, 1322
Bsp143I GATC 7 cut(s) 49, 236, 1051, 1249, 1270, 1285, 1291
Bsp1720I GCTNAGC 1 cut(s) 147
BspACI CCGC 4 cut(s) 582, 643, 953, 1375
BspANI GGCC 3 cut(s) 39, 754, 956
BspCNI CTCAG 2 cut(s) 139, 148
BspFNI CGCG 1 cut(s) 330
BspHI TCATGA 1 cut(s) 52
BspLI GGNNCC 2 cut(s) 468, 1044
BspMAI CTGCAG 1 cut(s) 145
BspPI GGATC 4 cut(s) 1059, 1278, 1286, 1293
BspTNI GGTCTC 1 cut(s) 1297
BsrDI GCAATG 2 cut(s) 803, 1307
BsrI ACTGG 2 cut(s) 201, 1369
BssECI CCNNGG 4 cut(s) 519, 577, 1031, 1046
BssMI GATC 7 cut(s) 49, 236, 1051, 1249, 1270, 1285, 1291
BssT1I CCWWGG 2 cut(s) 519, 1046
Bst2UI CCWGG 1 cut(s) 578
Bst4CI ACNGT 2 cut(s) 1092, 1185
Bst6I CTCTTC 1 cut(s) 765
BstBAI YACGTR 2 cut(s) 887, 970
BstC8I GCNNGC 3 cut(s) 20, 624, 1375
BstDEI CTNAG 5 cut(s) 26, 108, 135, 147, 152
BstDSI CCRYGG 1 cut(s) 1031
BstF5I GGATG 3 cut(s) 454, 739, 1171
BstFNI CGCG 1 cut(s) 330
BstHHI GCGC 1 cut(s) 440
BstKTI GATC 7 cut(s) 52, 239, 1054, 1252, 1273, 1288, 1294
BstMAI GTCTC 3 cut(s) 561, 762, 1297
BstMBI GATC 7 cut(s) 49, 236, 1051, 1249, 1270, 1285, 1291
BstMWI GCNNNNNNNGC 4 cut(s) 36, 176, 435, 970
BstNI CCWGG 1 cut(s) 578
BstNSI RCATGY 2 cut(s) 797, 917
BstSCI CCNGG 2 cut(s) 576, 1278
BstSFI CTRYAG 2 cut(s) 141, 1150
BstUI CGCG 1 cut(s) 330
BstV1I GCAGC 4 cut(s) 155, 661, 962, 1357
BstV2I GAAGAC 2 cut(s) 374, 1350
BstXI CCANNNNNNTGG 1 cut(s) 584
BsuRI GGCC 3 cut(s) 39, 754, 956
BtgI CCRYGG 1 cut(s) 1031
BtsCI GGATG 3 cut(s) 454, 739, 1171
BtsI GCAGTG 1 cut(s) 651
BtsIMutI CAGTG 2 cut(s) 651, 1362
Cac8I GCNNGC 3 cut(s) 20, 624, 1375
CaiI CAGNNNCTG 1 cut(s) 1096
CciI TCATGA 1 cut(s) 52
CfoI GCGC 1 cut(s) 440
Cfr13I GGNCC 1 cut(s) 955
CseI GACGC 1 cut(s) 11
Csp6I GTAC 3 cut(s) 162, 384, 788
CspCI CAANNNNNGTGG 2 cut(s) 241, 276
CviAII CATG 8 cut(s) 53, 206, 794, 800, 914, 959, 998, 1016
CviQI GTAC 3 cut(s) 162, 384, 788
DdeI CTNAG 5 cut(s) 26, 108, 135, 147, 152
DpnI GATC 7 cut(s) 51, 238, 1053, 1251, 1272, 1287, 1293
DpnII GATC 7 cut(s) 49, 236, 1051, 1249, 1270, 1285, 1291
DraI TTTAAA 3 cut(s) 553, 595, 856
Eam1104I CTCTTC 1 cut(s) 765
EarI CTCTTC 1 cut(s) 765
EciI GGCGGA 1 cut(s) 632
Ecl136II GAGCTC 1 cut(s) 1320
Eco130I CCWWGG 2 cut(s) 519, 1046
Eco147I AGGCCT 1 cut(s) 39
Eco24I GRGCYC 1 cut(s) 1322
Eco31I GGTCTC 1 cut(s) 1297
Eco53kI GAGCTC 1 cut(s) 1320
Eco57I CTGAAG 1 cut(s) 1118
Eco72I CACGTG 1 cut(s) 887
EcoICRI GAGCTC 1 cut(s) 1320
EcoRII CCWGG 1 cut(s) 576
EcoT14I CCWWGG 2 cut(s) 519, 1046
EcoT22I ATGCAT 2 cut(s) 805, 1006
EcoT38I GRGCYC 1 cut(s) 1322
ErhI CCWWGG 2 cut(s) 519, 1046
Esp3I CGTCTC 1 cut(s) 762
FaeI CATG 8 cut(s) 56, 209, 797, 803, 917, 962, 1001, 1019
FatI CATG 8 cut(s) 52, 205, 793, 799, 913, 958, 997, 1015
FauI CCCGC 1 cut(s) 1382
FauNDI CATATG 2 cut(s) 1066, 1246
FbaI TGATCA 1 cut(s) 1249
FblI GTMKAC 1 cut(s) 1156
Fnu4HI GCNGC 5 cut(s) 144, 675, 951, 954, 1371
FokI GGATG 3 cut(s) 461, 726, 1158
FriOI GRGCYC 1 cut(s) 1322
Fsp4HI GCNGC 5 cut(s) 144, 675, 951, 954, 1371
FspBI CTAG 6 cut(s) 45, 165, 222, 783, 947, 1047
GlaI GCGC 1 cut(s) 439
GluI GCNGC 5 cut(s) 144, 675, 951, 954, 1371
GsuI CTGGAG 1 cut(s) 1386
HaeIII GGCC 3 cut(s) 39, 754, 956
HapII CCGG 1 cut(s) 1280
HgaI GACGC 1 cut(s) 11
HhaI GCGC 1 cut(s) 440
Hin1II CATG 8 cut(s) 56, 209, 797, 803, 917, 962, 1001, 1019
Hin6I GCGC 1 cut(s) 438
HinP1I GCGC 1 cut(s) 438
HincII GTYRAC 1 cut(s) 1157
HindII GTYRAC 1 cut(s) 1157
HinfI GANTC 2 cut(s) 122, 935
HpaII CCGG 1 cut(s) 1280
HphI GGTGA 1 cut(s) 674
Hpy166II GTNNAC 4 cut(s) 386, 530, 1023, 1157
Hpy188I TCNGA 5 cut(s) 138, 448, 1132, 1275, 1302
Hpy188III TCNNGA 6 cut(s) 53, 539, 764, 1055, 1268, 1289
Hpy8I GTNNAC 4 cut(s) 386, 530, 1023, 1157
HpyAV CCTTC 3 cut(s) 725, 1005, 1093
HpyCH4III ACNGT 2 cut(s) 1092, 1185
HpyCH4IV ACGT 3 cut(s) 850, 886, 969
HpyCH4V TGCA 7 cut(s) 143, 497, 656, 803, 1004, 1195, 1312
HpyF10VI GCNNNNNNNGC 4 cut(s) 36, 176, 435, 970
HpyF3I CTNAG 5 cut(s) 26, 108, 135, 147, 152
HpySE526I ACGT 3 cut(s) 850, 886, 969
Hsp92II CATG 8 cut(s) 56, 209, 797, 803, 917, 962, 1001, 1019
HspAI GCGC 1 cut(s) 438
Ksp22I TGATCA 1 cut(s) 1249
Kzo9I GATC 7 cut(s) 49, 236, 1051, 1249, 1270, 1285, 1291
LmnI GCTCC 5 cut(s) 423, 452, 466, 1317, 1367
Lsp1109I GCAGC 4 cut(s) 155, 661, 962, 1357
LweI GCATC 4 cut(s) 665, 934, 991, 1220
MaeI CTAG 6 cut(s) 45, 165, 222, 783, 947, 1047
MaeII ACGT 3 cut(s) 850, 886, 969
MaeIII GTNAC 3 cut(s) 1086, 1197, 1224
MalI GATC 7 cut(s) 51, 238, 1053, 1251, 1272, 1287, 1293
MboI GATC 7 cut(s) 49, 236, 1051, 1249, 1270, 1285, 1291
MboII GAAGA 9 cut(s) 112, 117, 124, 189, 379, 740, 752, 1197, 1350
MfeI CAATTG 1 cut(s) 471
MhlI GDGCHC 2 cut(s) 628, 1322
MluCI AATT 4 cut(s) 172, 471, 488, 571
MmeI TCCRAC 1 cut(s) 767
Mph1103I ATGCAT 2 cut(s) 805, 1006
MroXI GAANNNNTTC 2 cut(s) 231, 735
MseI TTAA 5 cut(s) 510, 552, 594, 816, 855
MslI CAYNNNNRTG 2 cut(s) 687, 798
MspA1I CMGCKG 2 cut(s) 146, 953
MspI CCGG 1 cut(s) 1280
MspR9I CCNGG 2 cut(s) 578, 1280
MssI GTTTAAAC 1 cut(s) 595
MunI CAATTG 1 cut(s) 471
MvaI CCWGG 1 cut(s) 578
MvnI CGCG 1 cut(s) 330
MwoI GCNNNNNNNGC 4 cut(s) 36, 176, 435, 970
NciI CCSGG 1 cut(s) 1280
NdeI CATATG 2 cut(s) 1066, 1246
NdeII GATC 7 cut(s) 49, 236, 1051, 1249, 1270, 1285, 1291
NlaIII CATG 8 cut(s) 56, 209, 797, 803, 917, 962, 1001, 1019
NlaIV GGNNCC 2 cut(s) 468, 1044
NmuCI GTSAC 2 cut(s) 1086, 1197
NsiI ATGCAT 2 cut(s) 805, 1006
NspI RCATGY 2 cut(s) 797, 917
PagI TCATGA 1 cut(s) 52
PceI AGGCCT 1 cut(s) 39
PciI ACATGT 1 cut(s) 913
PcsI WCGNNNNNNNCGW 2 cut(s) 19, 1294
PdmI GAANNNNTTC 2 cut(s) 231, 735
PfeI GAWTC 2 cut(s) 122, 935
PkrI GCNGC 5 cut(s) 145, 676, 952, 955, 1372
PmaCI CACGTG 1 cut(s) 887
PmeI GTTTAAAC 1 cut(s) 595
PmlI CACGTG 1 cut(s) 887
Ppu21I YACGTR 2 cut(s) 887, 970
PscI ACATGT 1 cut(s) 913
Psp124BI GAGCTC 1 cut(s) 1322
Psp6I CCWGG 1 cut(s) 576
PspCI CACGTG 1 cut(s) 887
PspGI CCWGG 1 cut(s) 576
PspN4I GGNNCC 2 cut(s) 468, 1044
PspPI GGNCC 1 cut(s) 955
PstI CTGCAG 1 cut(s) 145
PstNI CAGNNNCTG 1 cut(s) 1096
PvuII CAGCTG 1 cut(s) 146
RsaI GTAC 3 cut(s) 163, 385, 789
RsaNI GTAC 3 cut(s) 162, 384, 788
RseI CAYNNNNRTG 2 cut(s) 687, 798
SacI GAGCTC 1 cut(s) 1322
SalI GTCGAC 1 cut(s) 1155
SaqAI TTAA 5 cut(s) 510, 552, 594, 816, 855
SatI GCNGC 5 cut(s) 144, 675, 951, 954, 1371
Sau3AI GATC 7 cut(s) 49, 236, 1051, 1249, 1270, 1285, 1291
Sau96I GGNCC 1 cut(s) 955
ScaI AGTACT 1 cut(s) 163
ScrFI CCNGG 2 cut(s) 578, 1280
SduI GDGCHC 2 cut(s) 628, 1322
SfaNI GCATC 4 cut(s) 665, 934, 991, 1220
SfcI CTRYAG 2 cut(s) 141, 1150
SmiMI CAYNNNNRTG 2 cut(s) 687, 798
SpeI ACTAGT 1 cut(s) 164
Sse9I AATT 4 cut(s) 172, 471, 488, 571
SseBI AGGCCT 1 cut(s) 39
SsiI CCGC 4 cut(s) 582, 643, 953, 1375
SspMI CTAG 6 cut(s) 45, 165, 222, 783, 947, 1047
SstI GAGCTC 1 cut(s) 1322
StuI AGGCCT 1 cut(s) 39
StyD4I CCNGG 2 cut(s) 576, 1278
StyI CCWWGG 2 cut(s) 519, 1046
TaaI ACNGT 2 cut(s) 1092, 1185
TaiI ACGT 3 cut(s) 853, 889, 972
TaqI TCGA 3 cut(s) 235, 910, 1156
TasI AATT 4 cut(s) 172, 471, 488, 571
TatI WGTACW 1 cut(s) 161
TauI GCSGC 1 cut(s) 956
TfiI GAWTC 2 cut(s) 122, 935
Tru1I TTAA 5 cut(s) 510, 552, 594, 816, 855
Tru9I TTAA 5 cut(s) 510, 552, 594, 816, 855
TscAI CASTG 2 cut(s) 658, 1369
TseFI GTSAC 2 cut(s) 1086, 1197
TseI GCWGC 4 cut(s) 143, 674, 950, 1370
Tsp45I GTSAC 2 cut(s) 1086, 1197
TspDTI ATGAA 4 cut(s) 728, 879, 927, 1053
TspRI CASTG 2 cut(s) 658, 1369
XapI RAATTY 1 cut(s) 488
XceI RCATGY 2 cut(s) 797, 917
XmaJI CCTAGG 1 cut(s) 1046
XmiI GTMKAC 1 cut(s) 1156
XmnI GAANNNNTTC 2 cut(s) 231, 735
XspI CTAG 6 cut(s) 45, 165, 222, 783, 947, 1047
ZrmI AGTACT 1 cut(s) 163
Zsp2I ATGCAT 2 cut(s) 805, 1006
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.