pycom06g12240

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0000847
Physical Location & Seq
Reverse (-)
17406283 .. 17408474
2192 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g12240.1

Sequence Viewer

Length: 1374 bp
ATGATCAAGGGAAGAAATGACGGGTTTGAAAACAAGGGGCCGGCTGCAAACTACATGAATGCCAACTATTACCCGGCTCGTGCTCCTTTGAATTATGCGTACGACCATCTTGCTGCTTCTGCTTGTGCTACTGAGGATCATGGGGTGCGTAAGGACCATCTGGGTGTGGAGGACTTTTCTGGCTATATATTTATGTGTAATGGAAGAACAAAACCGGAATGCTACCTGTACCGTGTCTTTGGACTTCCTGCTGGAAGAAGGGAAGTTATAGAGATGGTCAGGCCGGGCACAAAGCTGTTTCTATTTGATTATGATGTGAAGTTTCTTTATGGGGTATATGAAGCAACCACTGCTGGAAAGATGAACTTGGAAAGGACGGCTTTTGGTGGAAGCTTTCCTGCACAGGTAAGATTCAATACTTACAAGGAATGTCTCCCCTTGTCAGAGAGTGATTTTAAACATGCTATTCTAGATAACTACCAGAAAGGCTCCAAGAAATTTAACCCCATTCTCAATAGCCAACAGGTAAGCCATTTATTATCACTGTTTCATCCTATTACCACACAATCACCTGTCAAACATGACAGATCCTATAGAAAGGACCAATATCATCCCTACCCAAGGCTGCCTCCTTCAGGCACTACATACTTAAATTCAATGCGTCTCAGTCAACCCCCACAAGTGTTGGTGCCTCGGTATGTTCCACCGGTGGTCCTAGTTCAGCAGTGGGATGGTCGTGGATCCACAAGAAACATGGGTCTTTCCCATCATGCAGAGCACCCCGCAGTGTCCAATGCAGGTTCACAATCAGCAATTCCAACTCAATATACTGAGGAACAGTTTCAATCCCCAGCAAGCCTGCCTTCCGTGAAGGATGCAAATGCAGCCTCGGTATACAGCGGTCTTACTTCACCACACTTGGAGCCTCAGTATATTCCACCGAATGTCATACAGCAACAGCCTGTTAGCTATGGATACATATCATACATGGGCAACATTTATCCTGCTGTGCAACCGCAAGCTATGCCAGCTCCAAATCAGACATACTATTCTGCACAAGTTGCGCACAGTTACTCGGCTGAACTACCTGTCTCACAAGCGCCCACATCATACGAAAGCTATCACAGTCATGAGGCCACGCAGAATGCAGTTCCTAATGATCAACATACGAGCTTGGGGTACAGGTACAATCAGTTACCTTCGCAGGCGGAGACTGGAATACAGCAGGGGAATGCTGCCAAATACTACAGTCTCTACCAAGTGCCTACACTCCAGTATGTTTCATCACCTATACAAACACACCTCAACAATATTGGAAGCACCCGGGCTTCCAGCAGGGACTCTTCATCACTGAATTCCGCCCCAACCCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

458

Amino Acids

50.93

Weight (kDa)

8.41

Isoelectric Point (pI)

53.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 61 - 183 1.4e-46 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1065
Acc36I ACCTGC 1 cut(s) 788
AccB1I GGYRCC 1 cut(s) 688
AccI GTMKAC 1 cut(s) 894
AciI CCGC 5 cut(s) 783, 900, 1016, 1208, 1359
AclWI GGATC 4 cut(s) 144, 582, 735, 748
AcsI RAATTY 3 cut(s) 497, 652, 1354
AcuI CTGAAG 1 cut(s) 618
AfaI GTAC 4 cut(s) 101, 230, 1181, 1187
AfiI CCNNNNNNNGG 2 cut(s) 621, 635
AgeI ACCGGT 1 cut(s) 706
AgsI TTSAA 5 cut(s) 29, 91, 415, 657, 845
AluBI AGCT 7 cut(s) 295, 393, 969, 1022, 1031, 1119, 1173
AluI AGCT 7 cut(s) 295, 393, 969, 1022, 1031, 1119, 1173
Alw21I GWGCWC 2 cut(s) 85, 780
Alw26I GTCTC 5 cut(s) 437, 668, 1096, 1205, 1256
AlwI GGATC 4 cut(s) 144, 582, 735, 748
Ama87I CYCGRG 1 cut(s) 1323
AoxI GGCC 3 cut(s) 38, 281, 1134
ApeKI GCWGC 5 cut(s) 44, 113, 625, 884, 1235
ApoI RAATTY 3 cut(s) 497, 652, 1354
AsiGI ACCGGT 1 cut(s) 706
Asp700I GAANNNNTTC 1 cut(s) 840
AspLEI GCGC 2 cut(s) 1066, 1102
AspS9I GGNCC 4 cut(s) 38, 154, 601, 712
AsuC2I CCSGG 4 cut(s) 74, 285, 1324, 1325
AsuHPI GGTGA 3 cut(s) 561, 903, 1278
AvaI CYCGRG 1 cut(s) 1323
AvaII GGWCC 3 cut(s) 154, 601, 712
BaeGI GKGCMC 1 cut(s) 290
BamHI GGATCC 1 cut(s) 740
BanI GGYRCC 1 cut(s) 688
BauI CACGAG 1 cut(s) 78
Bbv12I GWGCWC 2 cut(s) 85, 780
BbvI GCAGC 5 cut(s) 31, 100, 612, 896, 1222
BccI CCATC 5 cut(s) 114, 165, 268, 725, 774
BceAI ACGGC 1 cut(s) 393
BcgI CGANNNNNNTGC 2 cut(s) 92, 126
BciVI GTATCC 1 cut(s) 968
BclI TGATCA 2 cut(s) 3, 1159
BcnI CCSGG 4 cut(s) 74, 285, 1324, 1325
BcoDI GTCTC 5 cut(s) 437, 668, 1096, 1205, 1256
BfaI CTAG 2 cut(s) 470, 716
BfmI CTRYAG 2 cut(s) 592, 1246
BfoI RGCGCY 1 cut(s) 1103
BfuAI ACCTGC 1 cut(s) 788
BfuI GTATCC 1 cut(s) 968
BisI GCNGC 5 cut(s) 45, 114, 626, 885, 1236
BlsI GCNGC 5 cut(s) 46, 115, 627, 886, 1237
Bme1390I CCNGG 4 cut(s) 74, 285, 1324, 1325
Bme18I GGWCC 3 cut(s) 154, 601, 712
BmeT110I CYCGRG 1 cut(s) 1323
BmgT120I GGNCC 4 cut(s) 38, 154, 601, 712
BmiI GGNNCC 5 cut(s) 39, 490, 690, 742, 924
BmrFI CCNGG 4 cut(s) 74, 285, 1324, 1325
BmsI GCATC 1 cut(s) 865
BpmI CTGGAG 1 cut(s) 1256
BpuMI CCSGG 4 cut(s) 74, 285, 1324, 1325
BsaBI GATNNNNATC 1 cut(s) 979
BsaJI CCNNGG 4 cut(s) 620, 692, 888, 1323
BsaWI WCCGGW 2 cut(s) 214, 706
BsaXI ACNNNNNCTCC 2 cut(s) 914, 944
Bsc4I CCNNNNNNNGG 2 cut(s) 621, 635
Bse118I RCCGGY 2 cut(s) 40, 706
Bse1I ACTGG 2 cut(s) 1219, 1273
Bse8I GATNNNNATC 1 cut(s) 979
BseDI CCNNGG 4 cut(s) 620, 692, 888, 1323
BseGI GGATG 4 cut(s) 550, 610, 736, 880
BseJI GATNNNNATC 1 cut(s) 979
BseLI CCNNNNNNNGG 2 cut(s) 621, 635
BseMII CTCAG 4 cut(s) 123, 679, 822, 941
BseNI ACTGG 2 cut(s) 1219, 1273
BseSI GKGCMC 1 cut(s) 290
BseXI GCAGC 5 cut(s) 31, 100, 612, 896, 1222
BseYI CCCAGC 1 cut(s) 850
BsgI GTGCAG 2 cut(s) 384, 1038
BshFI GGCC 3 cut(s) 40, 283, 1136
BshNI GGYRCC 1 cut(s) 688
BshTI ACCGGT 1 cut(s) 706
BsiHKAI GWGCWC 2 cut(s) 85, 780
BsiHKCI CYCGRG 1 cut(s) 1323
BsiSI CCGG 6 cut(s) 41, 74, 215, 284, 707, 1324
BsiWI CGTACG 1 cut(s) 99
BslFI GGGAC 1 cut(s) 1352
BslI CCNNNNNNNGG 2 cut(s) 621, 635
BsmAI GTCTC 5 cut(s) 437, 668, 1096, 1205, 1256
BsmBI CGTCTC 1 cut(s) 668
BsmFI GGGAC 1 cut(s) 1352
BsmI GAATGC 4 cut(s) 64, 224, 1150, 1237
BsnI GGCC 3 cut(s) 40, 283, 1136
BsoBI CYCGRG 1 cut(s) 1323
Bsp1286I GDGCHC 3 cut(s) 85, 290, 780
Bsp143I GATC 5 cut(s) 3, 136, 587, 740, 1159
BspACI CCGC 5 cut(s) 783, 900, 1016, 1208, 1359
BspANI GGCC 3 cut(s) 40, 283, 1136
BspCNI CTCAG 4 cut(s) 124, 678, 823, 940
BspHI TCATGA 1 cut(s) 1129
BspLI GGNNCC 5 cut(s) 39, 490, 690, 742, 924
BspMI ACCTGC 1 cut(s) 788
BspPI GGATC 4 cut(s) 144, 582, 735, 748
BspT107I GGYRCC 1 cut(s) 688
BsrFI RCCGGY 2 cut(s) 40, 706
BsrI ACTGG 2 cut(s) 1219, 1273
BssAI RCCGGY 2 cut(s) 40, 706
BssECI CCNNGG 4 cut(s) 620, 692, 888, 1323
BssMI GATC 5 cut(s) 3, 136, 587, 740, 1159
BssNAI GTATAC 1 cut(s) 895
BssSI CACGAG 1 cut(s) 78
BssT1I CCWWGG 1 cut(s) 620
Bst1107I GTATAC 1 cut(s) 895
Bst2BI CACGAG 1 cut(s) 78
Bst4CI ACNGT 6 cut(s) 233, 546, 840, 1070, 1127, 1250
Bst6I CTCTTC 1 cut(s) 1348
BstAPI GCANNNNNTGC 3 cut(s) 350, 1024, 1061
BstC8I GCNNGC 6 cut(s) 42, 856, 860, 1020, 1029, 1206
BstDEI CTNAG 4 cut(s) 132, 665, 831, 927
BstENI CCTNNNNNAGG 2 cut(s) 619, 633
BstF5I GGATG 4 cut(s) 550, 610, 736, 880
BstH2I RGCGCY 1 cut(s) 1103
BstHHI GCGC 2 cut(s) 1066, 1102
BstKTI GATC 5 cut(s) 6, 139, 590, 743, 1162
BstMAI GTCTC 5 cut(s) 437, 668, 1096, 1205, 1256
BstMBI GATC 5 cut(s) 3, 136, 587, 740, 1159
BstMWI GCNNNNNNNGC 6 cut(s) 119, 350, 884, 1024, 1028, 1061
BstNSI RCATGY 1 cut(s) 464
BstSCI CCNGG 4 cut(s) 72, 283, 1322, 1323
BstSFI CTRYAG 2 cut(s) 592, 1246
BstSLI GKGCMC 1 cut(s) 290
BstV1I GCAGC 5 cut(s) 31, 100, 612, 896, 1222
BstX2I RGATCY 2 cut(s) 587, 740
BstYI RGATCY 2 cut(s) 587, 740
BstZ17I GTATAC 1 cut(s) 895
BsuI GTATCC 1 cut(s) 968
BsuRI GGCC 3 cut(s) 40, 283, 1136
BtsCI GGATG 4 cut(s) 550, 610, 736, 880
BtsI GCAGTG 3 cut(s) 348, 731, 792
BtsIMutI CAGTG 5 cut(s) 348, 542, 731, 792, 1349
BveI ACCTGC 1 cut(s) 788
Cac8I GCNNGC 6 cut(s) 42, 856, 860, 1020, 1029, 1206
CciI TCATGA 1 cut(s) 1129
CfoI GCGC 2 cut(s) 1066, 1102
Cfr10I RCCGGY 2 cut(s) 40, 706
Cfr13I GGNCC 4 cut(s) 38, 154, 601, 712
Cfr9I CCCGGG 1 cut(s) 1323
CseI GACGC 1 cut(s) 650
Csp6I GTAC 4 cut(s) 100, 229, 1180, 1186
CspAI ACCGGT 1 cut(s) 706
CspCI CAANNNNNGTGG 2 cut(s) 666, 701
CviAII CATG 8 cut(s) 55, 140, 461, 581, 754, 770, 988, 1130
CviQI GTAC 4 cut(s) 100, 229, 1180, 1186
DdeI CTNAG 4 cut(s) 132, 665, 831, 927
DpnI GATC 5 cut(s) 5, 138, 589, 742, 1161
DpnII GATC 5 cut(s) 3, 136, 587, 740, 1159
DraI TTTAAA 1 cut(s) 457
Eam1104I CTCTTC 1 cut(s) 1348
EarI CTCTTC 1 cut(s) 1348
EciI GGCGGA 2 cut(s) 1223, 1348
Eco130I CCWWGG 1 cut(s) 620
Eco47I GGWCC 3 cut(s) 154, 601, 712
Eco57I CTGAAG 1 cut(s) 618
Eco88I CYCGRG 1 cut(s) 1323
EcoNI CCTNNNNNAGG 2 cut(s) 619, 633
EcoRI GAATTC 1 cut(s) 1354
EcoT14I CCWWGG 1 cut(s) 620
ErhI CCWWGG 1 cut(s) 620
Esp3I CGTCTC 1 cut(s) 668
FaeI CATG 8 cut(s) 58, 143, 464, 584, 757, 773, 991, 1133
FalI AAGNNNNNCTT 6 cut(s) 350, 382, 364, 396, 847, 879
FaqI GGGAC 1 cut(s) 1352
FatI CATG 8 cut(s) 54, 139, 460, 580, 753, 769, 987, 1129
FauI CCCGC 1 cut(s) 790
FbaI TGATCA 2 cut(s) 3, 1159
FblI GTMKAC 1 cut(s) 894
Fnu4HI GCNGC 5 cut(s) 45, 114, 626, 885, 1236
FokI GGATG 4 cut(s) 537, 597, 743, 887
Fsp4HI GCNGC 5 cut(s) 45, 114, 626, 885, 1236
FspBI CTAG 2 cut(s) 470, 716
FspI TGCGCA 1 cut(s) 1065
GlaI GCGC 2 cut(s) 1065, 1101
GluI GCNGC 5 cut(s) 45, 114, 626, 885, 1236
GsaI CCCAGC 1 cut(s) 854
GsuI CTGGAG 1 cut(s) 1256
HaeII RGCGCY 1 cut(s) 1103
HaeIII GGCC 3 cut(s) 40, 283, 1136
HapII CCGG 6 cut(s) 41, 74, 215, 284, 707, 1324
HgaI GACGC 1 cut(s) 650
HhaI GCGC 2 cut(s) 1066, 1102
Hin1II CATG 8 cut(s) 58, 143, 464, 584, 757, 773, 991, 1133
Hin6I GCGC 2 cut(s) 1064, 1100
HinP1I GCGC 2 cut(s) 1064, 1100
HincII GTYRAC 1 cut(s) 671
HindII GTYRAC 1 cut(s) 671
HindIII AAGCTT 1 cut(s) 391
HinfI GANTC 2 cut(s) 411, 1340
HpaII CCGG 6 cut(s) 41, 74, 215, 284, 707, 1324
HphI GGTGA 3 cut(s) 561, 903, 1278
Hpy166II GTNNAC 3 cut(s) 671, 803, 895
Hpy188I TCNGA 2 cut(s) 445, 1041
Hpy188III TCNNGA 2 cut(s) 470, 1130
Hpy8I GTNNAC 3 cut(s) 671, 803, 895
HpyAV CCTTC 5 cut(s) 252, 642, 865, 873, 1209
HpyCH4III ACNGT 6 cut(s) 233, 546, 840, 1070, 1127, 1250
HpyCH4V TGCA 9 cut(s) 47, 401, 773, 797, 878, 884, 1012, 1055, 1148
HpyF10VI GCNNNNNNNGC 6 cut(s) 119, 350, 884, 1024, 1028, 1061
HpyF3I CTNAG 4 cut(s) 132, 665, 831, 927
Hsp92II CATG 8 cut(s) 58, 143, 464, 584, 757, 773, 991, 1133
HspAI GCGC 2 cut(s) 1064, 1100
KroI GCCGGC 1 cut(s) 40
KroNI GCCGGC 1 cut(s) 42
Ksp22I TGATCA 2 cut(s) 3, 1159
Kzo9I GATC 5 cut(s) 3, 136, 587, 740, 1159
LmnI GCTCC 4 cut(s) 88, 494, 922, 1036
Lsp1109I GCAGC 5 cut(s) 31, 100, 612, 896, 1222
LweI GCATC 1 cut(s) 865
MaeI CTAG 2 cut(s) 470, 716
MaeIII GTNAC 2 cut(s) 1070, 1194
MalI GATC 5 cut(s) 5, 138, 589, 742, 1161
MboI GATC 5 cut(s) 3, 136, 587, 740, 1159
MboII GAAGA 4 cut(s) 24, 216, 267, 1335
MflI RGATCY 2 cut(s) 587, 740
MhlI GDGCHC 3 cut(s) 85, 290, 780
MluCI AATT 5 cut(s) 91, 497, 652, 813, 1354
MlyI GAGTC 1 cut(s) 1334
MmeI TCCRAC 1 cut(s) 842
MnlI CCTC 9 cut(s) 127, 163, 639, 702, 826, 898, 936, 1126, 1313
MroNI GCCGGC 1 cut(s) 40
MroXI GAANNNNTTC 1 cut(s) 840
MseI TTAA 3 cut(s) 456, 501, 650
MslI CAYNNNNRTG 2 cut(s) 162, 1128
MspA1I CMGCKG 1 cut(s) 900
MspI CCGG 6 cut(s) 41, 74, 215, 284, 707, 1324
MspR9I CCNGG 4 cut(s) 74, 285, 1324, 1325
Mva1269I GAATGC 4 cut(s) 64, 224, 1150, 1237
MwoI GCNNNNNNNGC 6 cut(s) 119, 350, 884, 1024, 1028, 1061
NaeI GCCGGC 1 cut(s) 42
NciI CCSGG 4 cut(s) 74, 285, 1324, 1325
NdeII GATC 5 cut(s) 3, 136, 587, 740, 1159
NgoMIV GCCGGC 1 cut(s) 40
NlaIII CATG 8 cut(s) 58, 143, 464, 584, 757, 773, 991, 1133
NlaIV GGNNCC 5 cut(s) 39, 490, 690, 742, 924
NmeAIII GCCGAG 1 cut(s) 1055
NsbI TGCGCA 1 cut(s) 1065
NspI RCATGY 1 cut(s) 464
PagI TCATGA 1 cut(s) 1129
PctI GAATGC 4 cut(s) 64, 224, 1150, 1237
PdiI GCCGGC 1 cut(s) 42
PdmI GAANNNNTTC 1 cut(s) 840
PfeI GAWTC 1 cut(s) 411
Pfl23II CGTACG 1 cut(s) 99
PinAI ACCGGT 1 cut(s) 706
PkrI GCNGC 5 cut(s) 46, 115, 627, 886, 1237
PleI GAGTC 1 cut(s) 1334
PpsI GAGTC 1 cut(s) 1334
PspFI CCCAGC 1 cut(s) 850
PspLI CGTACG 1 cut(s) 99
PspN4I GGNNCC 5 cut(s) 39, 490, 690, 742, 924
PspPI GGNCC 4 cut(s) 38, 154, 601, 712
PsuI RGATCY 2 cut(s) 587, 740
RsaI GTAC 4 cut(s) 101, 230, 1181, 1187
RsaNI GTAC 4 cut(s) 100, 229, 1180, 1186
RseI CAYNNNNRTG 2 cut(s) 162, 1128
SaqAI TTAA 3 cut(s) 456, 501, 650
SatI GCNGC 5 cut(s) 45, 114, 626, 885, 1236
Sau3AI GATC 5 cut(s) 3, 136, 587, 740, 1159
Sau96I GGNCC 4 cut(s) 38, 154, 601, 712
SchI GAGTC 1 cut(s) 1334
ScrFI CCNGG 4 cut(s) 74, 285, 1324, 1325
SduI GDGCHC 3 cut(s) 85, 290, 780
SfaNI GCATC 1 cut(s) 865
SfcI CTRYAG 2 cut(s) 592, 1246
SgrAI CRCCGGYG 1 cut(s) 706
SinI GGWCC 3 cut(s) 154, 601, 712
SmaI CCCGGG 1 cut(s) 1325
SmiMI CAYNNNNRTG 2 cut(s) 162, 1128
Sse9I AATT 5 cut(s) 91, 497, 652, 813, 1354
SsiI CCGC 5 cut(s) 783, 900, 1016, 1208, 1359
SspI AATATT 1 cut(s) 1312
SspMI CTAG 2 cut(s) 470, 716
StyD4I CCNGG 4 cut(s) 72, 283, 1322, 1323
StyI CCWWGG 1 cut(s) 620
TaaI ACNGT 6 cut(s) 233, 546, 840, 1070, 1127, 1250
TasI AATT 5 cut(s) 91, 497, 652, 813, 1354
TfiI GAWTC 1 cut(s) 411
Tru1I TTAA 3 cut(s) 456, 501, 650
Tru9I TTAA 3 cut(s) 456, 501, 650
TscAI CASTG 5 cut(s) 355, 549, 731, 792, 1356
TseI GCWGC 5 cut(s) 44, 113, 625, 884, 1235
TspDTI ATGAA 6 cut(s) 71, 354, 377, 539, 1272, 1335
TspGWI ACGGA 1 cut(s) 856
TspMI CCCGGG 1 cut(s) 1323
TspRI CASTG 5 cut(s) 355, 549, 731, 792, 1356
VpaK11BI GGWCC 3 cut(s) 154, 601, 712
XagI CCTNNNNNAGG 2 cut(s) 619, 633
XapI RAATTY 3 cut(s) 497, 652, 1354
XbaI TCTAGA 1 cut(s) 469
XceI RCATGY 1 cut(s) 464
XcmI CCANNNNNNNNNTGG 1 cut(s) 751
XmaI CCCGGG 1 cut(s) 1323
XmiI GTMKAC 1 cut(s) 894
XmnI GAANNNNTTC 1 cut(s) 840
XspI CTAG 2 cut(s) 470, 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.