pycom16g11120

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0000847
Physical Location & Seq
Forward (+)
7718568 .. 7721989
3422 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g11120.3

Sequence Viewer

Length: 1398 bp
ATGGCAAAGAAAAGAGGGAAGGCGTTAAAGGGAAAAGATGTAGCTGTAAATTACAATGATAGGAATAAGAAGATAGAGAAGAAATTGACGAAGAAAAAGAAGAAATCTATGAAGATAAGTGCATCTTCAGTACCTACGTCGGCTCCTGTGCCTAACAGCGGCCCTTCCACTTCCAATGTTGCTCATGCGACTTCGTCTAGCCATGCTAATCCTACTGGTAGAAAAAAGCTTCGGCCAACAAAGAATGAGAAGGATAGTGGGCACTCTCATTCCGGTTTTATATTTATGTGTAGTGGGAAAACGAAACCCGAATGTTATCGCTATCGTGTTTTTGGGCTGCCATTAGCAAAGGTGGACGTTGTGAAAAAAATTAGACCGGGTGCAAAGCTATTTCTGTATGATTTTGACTTGAAGCTTCTTTATGGCACATACAAGGCAACTTCAAATGGAGGAGTGGATTTGGAACCAATTGCGTTTAAGGGGAAATTCCCTGCACAGGTCAGATTTAAGATTTTCAAGGATTGTTTACCTCTTCCTGAAAATGCTTTTAAAGCAGCTATCAGAGACAACTATCAGGGAGGTTCTAAGTTTAAACAAGAGCTCAATAGCAAACAGGTGCATGCTTTAATCTCATTGTTTCGCCCACTCACCGAGTCAGTACCTACTTCTGCTGCCCCTCCCCGAGATGTGGCAAAACCAGGCTCATTTCCATCTCCCATTACAGAAGAAGGATTTCATCCAACAGCAAGGCTGTCCCATCAAGAGGGCTCCTATTCATATGGTATGCAACGTACACGTGCATCGCCGCTTGATATGCAATCCAGGCAGCCAATCCCATATCCACTTTATGATCAATATGAGGTCGAAACACATGTGGGACATGTATGGCCTCGCTTAGAGCCTCGACATGTTCAGCATGCTTCTCTTCCACGTCGTGCTGATTCCTATTATTTAGCAGTGACACATGAGCCGTACTTGCCTGAACAACCATACATGTCCCATCAGGATGCATATAGAAGGCATAGGGTGACACTTGCAGAGGAGATGGTTCCCAGGGATCAAGTTGTTGCCTATGGAAGTGAGTATGACGGGCCACAGTTGCTGAAGCGGAGAGAGAGAGAAATTGCTCCGCGTTCCAATTATGTGGCTGAATCTTATAGTCGAGAGGTTCCCACTGCTGCAGCCTCACATGTTATGTTGCAGTCGCATTCTTTAGCACCATCTTATGAACGGACGTTAACGTACCAACGGTACTATCCAGTCACAACCCTTCAGGATCAGAGCTTGGTGTACGCTGACCCTCTTGTAAGACCATTGCACGGTACCTCTTACTCAGCTGAGGCAGATGTTCCAATCTCTACTCACTTTTCGTACACTAGAGCACCCCATTACCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

466

Amino Acids

52.5

Weight (kDa)

9.74

Isoelectric Point (pI)

44.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1322
AccB1I GGYRCC 1 cut(s) 1322
AccII CGCG 1 cut(s) 1132
AciI CCGC 5 cut(s) 159, 806, 1108, 1130, 1393
AclWI GGATC 2 cut(s) 1065, 1284
AcoI YGGCCR 1 cut(s) 233
AcsI RAATTY 1 cut(s) 485
AcuI CTGAAG 3 cut(s) 111, 1124, 1256
AcvI CACGTG 1 cut(s) 797
AdeI CACNNNGTG 1 cut(s) 935
AfaI GTAC 9 cut(s) 132, 660, 793, 974, 1244, 1253, 1292, 1324, 1373
AfiI CCNNNNNNNGG 5 cut(s) 158, 496, 688, 763, 1319
AflIII ACRYGT 6 cut(s) 794, 871, 880, 907, 993, 1189
AgsI TTSAA 3 cut(s) 412, 444, 517
AjiI CACGTC 1 cut(s) 932
AjnI CCWGG 3 cut(s) 697, 821, 1052
AluBI AGCT 8 cut(s) 44, 229, 388, 415, 557, 601, 1284, 1337
AluI AGCT 8 cut(s) 44, 229, 388, 415, 557, 601, 1284, 1337
Alw21I GWGCWC 2 cut(s) 603, 1384
Alw26I GTCTC 1 cut(s) 558
AlwI GGATC 2 cut(s) 1065, 1284
AlwNI CAGNNNCTG 1 cut(s) 1102
Ama87I CYCGRG 1 cut(s) 681
AoxI GGCC 4 cut(s) 160, 233, 887, 1091
ApeKI GCWGC 6 cut(s) 337, 554, 671, 826, 1178, 1181
ApoI RAATTY 1 cut(s) 485
Asp700I GAANNNNTTC 1 cut(s) 732
Asp718I GGTACC 1 cut(s) 1322
AspS9I GGNCC 2 cut(s) 161, 1091
AsuC2I CCSGG 1 cut(s) 378
AsuHPI GGTGA 2 cut(s) 640, 1039
AvaI CYCGRG 1 cut(s) 681
BaeGI GKGCMC 1 cut(s) 264
BanI GGYRCC 1 cut(s) 1322
BanII GRGCYC 2 cut(s) 603, 770
BbrPI CACGTG 1 cut(s) 797
Bbv12I GWGCWC 2 cut(s) 603, 1384
BbvCI CCTCAGC 1 cut(s) 1338
BbvI GCAGC 6 cut(s) 324, 566, 658, 838, 1165, 1193
BccI CCATC 5 cut(s) 718, 765, 1008, 1039, 1228
BceAI ACGGC 1 cut(s) 955
BciT130I CCWGG 3 cut(s) 699, 823, 1054
BclI TGATCA 1 cut(s) 850
BcnI CCSGG 1 cut(s) 378
BcoDI GTCTC 1 cut(s) 558
BfaI CTAG 2 cut(s) 198, 1377
BfmI CTRYAG 1 cut(s) 1179
BisI GCNGC 8 cut(s) 160, 338, 555, 672, 806, 827, 1179, 1182
BlsI GCNGC 8 cut(s) 161, 339, 556, 673, 807, 828, 1180, 1183
Bme1390I CCNGG 4 cut(s) 378, 699, 823, 1054
BmeT110I CYCGRG 1 cut(s) 681
BmgBI CACGTC 1 cut(s) 932
BmgT120I GGNCC 2 cut(s) 161, 1091
BmiI GGNNCC 6 cut(s) 144, 465, 769, 1050, 1170, 1324
BmrFI CCNGG 4 cut(s) 378, 699, 823, 1054
BmsI GCATC 3 cut(s) 131, 809, 997
Bpu10I CCTNAGC 1 cut(s) 1338
BpuMI CCSGG 1 cut(s) 378
BsaAI YACGTR 1 cut(s) 797
BsaJI CCNNGG 2 cut(s) 1052, 1053
BsaWI WCCGGW 1 cut(s) 272
BsaXI ACNNNNNCTCC 2 cut(s) 127, 157
Bsc4I CCNNNNNNNGG 5 cut(s) 158, 496, 688, 763, 1319
Bse1I ACTGG 2 cut(s) 220, 1259
Bse3DI GCAATG 1 cut(s) 1313
BseBI CCWGG 3 cut(s) 699, 823, 1054
BseDI CCNNGG 2 cut(s) 1052, 1053
BseGI GGATG 2 cut(s) 736, 1012
BseLI CCNNNNNNNGG 5 cut(s) 158, 496, 688, 763, 1319
BseMI GCAATG 1 cut(s) 1313
BseMII CTCAG 2 cut(s) 1329, 1347
BseNI ACTGG 2 cut(s) 220, 1259
BseRI GAGGAG 2 cut(s) 465, 1055
BseSI GKGCMC 1 cut(s) 264
BseXI GCAGC 6 cut(s) 324, 566, 658, 838, 1165, 1193
BsgI GTGCAG 1 cut(s) 477
Bsh1236I CGCG 1 cut(s) 1132
BshFI GGCC 4 cut(s) 162, 235, 889, 1093
BshNI GGYRCC 1 cut(s) 1322
BsiHKAI GWGCWC 2 cut(s) 603, 1384
BsiHKCI CYCGRG 1 cut(s) 681
BsiSI CCGG 2 cut(s) 273, 377
BslFI GGGAC 3 cut(s) 739, 891, 982
BslI CCNNNNNNNGG 5 cut(s) 158, 496, 688, 763, 1319
BsmAI GTCTC 1 cut(s) 558
BsmFI GGGAC 3 cut(s) 739, 891, 982
BsmI GAATGC 1 cut(s) 1207
BsnI GGCC 4 cut(s) 162, 235, 889, 1093
BsoBI CYCGRG 1 cut(s) 681
Bsp1286I GDGCHC 4 cut(s) 264, 603, 770, 1384
Bsp143I GATC 3 cut(s) 850, 1057, 1276
BspACI CCGC 5 cut(s) 159, 806, 1108, 1130, 1393
BspANI GGCC 4 cut(s) 162, 235, 889, 1093
BspCNI CTCAG 2 cut(s) 1330, 1346
BspFNI CGCG 1 cut(s) 1132
BspLI GGNNCC 6 cut(s) 144, 465, 769, 1050, 1170, 1324
BspMAI CTGCAG 1 cut(s) 1183
BspPI GGATC 2 cut(s) 1065, 1284
BspT107I GGYRCC 1 cut(s) 1322
BsrDI GCAATG 1 cut(s) 1313
BsrI ACTGG 2 cut(s) 220, 1259
BssECI CCNNGG 2 cut(s) 1052, 1053
BssMI GATC 3 cut(s) 850, 1057, 1276
Bst2UI CCWGG 3 cut(s) 699, 823, 1054
Bst4CI ACNGT 3 cut(s) 1098, 1251, 1322
Bst6I CTCTTC 2 cut(s) 537, 930
BstBAI YACGTR 1 cut(s) 797
BstC8I GCNNGC 2 cut(s) 621, 918
BstDEI CTNAG 4 cut(s) 585, 895, 1333, 1338
BstF5I GGATG 2 cut(s) 736, 1012
BstFNI CGCG 1 cut(s) 1132
BstKTI GATC 3 cut(s) 853, 1060, 1279
BstMAI GTCTC 1 cut(s) 558
BstMBI GATC 3 cut(s) 850, 1057, 1276
BstMWI GCNNNNNNNGC 5 cut(s) 551, 814, 823, 976, 1099
BstNI CCWGG 3 cut(s) 699, 823, 1054
BstNSI RCATGY 7 cut(s) 623, 875, 884, 911, 920, 997, 1193
BstSCI CCNGG 4 cut(s) 376, 697, 821, 1052
BstSFI CTRYAG 1 cut(s) 1179
BstSLI GKGCMC 1 cut(s) 264
BstUI CGCG 1 cut(s) 1132
BstV1I GCAGC 6 cut(s) 324, 566, 658, 838, 1165, 1193
BstXI CCANNNNNNTGG 1 cut(s) 1144
BsuRI GGCC 4 cut(s) 162, 235, 889, 1093
BtgZI GCGATG 1 cut(s) 786
BtrI CACGTC 1 cut(s) 932
BtsCI GGATG 2 cut(s) 736, 1012
BtsI GCAGTG 2 cut(s) 963, 1173
BtsIMutI CAGTG 2 cut(s) 963, 1173
Cac8I GCNNGC 2 cut(s) 621, 918
CaiI CAGNNNCTG 1 cut(s) 1102
Cfr13I GGNCC 2 cut(s) 161, 1091
Csp6I GTAC 9 cut(s) 131, 659, 792, 973, 1243, 1252, 1291, 1323, 1372
CviQI GTAC 9 cut(s) 131, 659, 792, 973, 1243, 1252, 1291, 1323, 1372
DdeI CTNAG 4 cut(s) 585, 895, 1333, 1338
DpnI GATC 3 cut(s) 852, 1059, 1278
DpnII GATC 3 cut(s) 850, 1057, 1276
DraI TTTAAA 2 cut(s) 550, 592
DraIII CACNNNGTG 1 cut(s) 935
EaeI YGGCCR 1 cut(s) 233
Eam1104I CTCTTC 2 cut(s) 537, 930
EarI CTCTTC 2 cut(s) 537, 930
Ecl136II GAGCTC 1 cut(s) 601
Eco24I GRGCYC 2 cut(s) 603, 770
Eco53kI GAGCTC 1 cut(s) 601
Eco57I CTGAAG 3 cut(s) 111, 1124, 1256
Eco72I CACGTG 1 cut(s) 797
Eco88I CYCGRG 1 cut(s) 681
EcoICRI GAGCTC 1 cut(s) 601
EcoRII CCWGG 3 cut(s) 697, 821, 1052
EcoT22I ATGCAT 1 cut(s) 1012
EcoT38I GRGCYC 2 cut(s) 603, 770
FalI AAGNNNNNCTT 2 cut(s) 109, 141
FaqI GGGAC 3 cut(s) 739, 891, 982
FauNDI CATATG 1 cut(s) 778
FbaI TGATCA 1 cut(s) 850
Fnu4HI GCNGC 8 cut(s) 160, 338, 555, 672, 806, 827, 1179, 1182
FokI GGATG 2 cut(s) 723, 1019
FriOI GRGCYC 2 cut(s) 603, 770
Fsp4HI GCNGC 8 cut(s) 160, 338, 555, 672, 806, 827, 1179, 1182
FspBI CTAG 2 cut(s) 198, 1377
GluI GCNGC 8 cut(s) 160, 338, 555, 672, 806, 827, 1179, 1182
HaeIII GGCC 4 cut(s) 162, 235, 889, 1093
HapII CCGG 2 cut(s) 273, 377
HincII GTYRAC 1 cut(s) 1239
HindII GTYRAC 1 cut(s) 1239
HindIII AAGCTT 2 cut(s) 227, 413
HinfI GANTC 3 cut(s) 653, 941, 1151
HpaI GTTAAC 1 cut(s) 1239
HpaII CCGG 2 cut(s) 273, 377
HphI GGTGA 2 cut(s) 640, 1039
Hpy166II GTNNAC 6 cut(s) 355, 527, 794, 1239, 1291, 1374
Hpy188I TCNGA 3 cut(s) 503, 563, 1281
Hpy188III TCNNGA 5 cut(s) 536, 761, 1004, 1163, 1274
Hpy8I GTNNAC 6 cut(s) 355, 527, 794, 1239, 1291, 1374
Hpy99I CGWCG 2 cut(s) 142, 936
HpyAV CCTTC 6 cut(s) 13, 174, 244, 722, 1011, 1280
HpyCH4III ACNGT 3 cut(s) 1098, 1251, 1322
HpyCH4IV ACGT 7 cut(s) 137, 357, 790, 796, 931, 1235, 1241
HpyF10VI GCNNNNNNNGC 5 cut(s) 551, 814, 823, 976, 1099
HpyF3I CTNAG 4 cut(s) 585, 895, 1333, 1338
HpySE526I ACGT 7 cut(s) 137, 357, 790, 796, 931, 1235, 1241
KpnI GGTACC 1 cut(s) 1326
Ksp22I TGATCA 1 cut(s) 850
KspAI GTTAAC 1 cut(s) 1239
Kzo9I GATC 3 cut(s) 850, 1057, 1276
LmnI GCTCC 3 cut(s) 148, 773, 1132
Lsp1109I GCAGC 6 cut(s) 324, 566, 658, 838, 1165, 1193
LweI GCATC 3 cut(s) 131, 809, 997
MaeI CTAG 2 cut(s) 198, 1377
MaeII ACGT 7 cut(s) 137, 357, 790, 796, 931, 1235, 1241
MaeIII GTNAC 3 cut(s) 958, 1027, 1261
MalI GATC 3 cut(s) 852, 1059, 1278
MboI GATC 3 cut(s) 850, 1057, 1276
MboII GAAGA 9 cut(s) 82, 91, 103, 112, 117, 124, 524, 737, 917
MfeI CAATTG 1 cut(s) 468
MhlI GDGCHC 4 cut(s) 264, 603, 770, 1384
MluCI AATT 7 cut(s) 49, 83, 369, 468, 485, 1122, 1138
MlyI GAGTC 1 cut(s) 662
MmeI TCCRAC 1 cut(s) 764
Mph1103I ATGCAT 1 cut(s) 1012
MroXI GAANNNNTTC 1 cut(s) 732
MseI TTAA 7 cut(s) 26, 477, 507, 549, 591, 626, 1238
MslI CAYNNNNRTG 1 cut(s) 1005
MspA1I CMGCKG 3 cut(s) 159, 1337, 1395
MspI CCGG 2 cut(s) 273, 377
MspR9I CCNGG 4 cut(s) 378, 699, 823, 1054
MssI GTTTAAAC 1 cut(s) 592
MunI CAATTG 1 cut(s) 468
Mva1269I GAATGC 1 cut(s) 1207
MvaI CCWGG 3 cut(s) 699, 823, 1054
MvnI CGCG 1 cut(s) 1132
MwoI GCNNNNNNNGC 5 cut(s) 551, 814, 823, 976, 1099
NciI CCSGG 1 cut(s) 378
NdeI CATATG 1 cut(s) 778
NdeII GATC 3 cut(s) 850, 1057, 1276
NlaIV GGNNCC 6 cut(s) 144, 465, 769, 1050, 1170, 1324
NmuCI GTSAC 3 cut(s) 958, 1027, 1261
NsiI ATGCAT 1 cut(s) 1012
NspI RCATGY 7 cut(s) 623, 875, 884, 911, 920, 997, 1193
PaeI GCATGC 2 cut(s) 623, 920
PasI CCCWGGG 1 cut(s) 1053
PciI ACATGT 5 cut(s) 871, 880, 907, 993, 1189
PctI GAATGC 1 cut(s) 1207
PdmI GAANNNNTTC 1 cut(s) 732
PfeI GAWTC 2 cut(s) 941, 1151
PflFI GACNNNGTC 1 cut(s) 193
PkrI GCNGC 8 cut(s) 161, 339, 556, 673, 807, 828, 1180, 1183
PleI GAGTC 1 cut(s) 661
PmaCI CACGTG 1 cut(s) 797
PmeI GTTTAAAC 1 cut(s) 592
PmlI CACGTG 1 cut(s) 797
PpsI GAGTC 1 cut(s) 661
Ppu21I YACGTR 1 cut(s) 797
PscI ACATGT 5 cut(s) 871, 880, 907, 993, 1189
Psp124BI GAGCTC 1 cut(s) 603
Psp6I CCWGG 3 cut(s) 697, 821, 1052
PspCI CACGTG 1 cut(s) 797
PspGI CCWGG 3 cut(s) 697, 821, 1052
PspN4I GGNNCC 6 cut(s) 144, 465, 769, 1050, 1170, 1324
PspPI GGNCC 2 cut(s) 161, 1091
PstI CTGCAG 1 cut(s) 1183
PstNI CAGNNNCTG 1 cut(s) 1102
PsyI GACNNNGTC 1 cut(s) 193
PvuII CAGCTG 1 cut(s) 1337
RsaI GTAC 9 cut(s) 132, 660, 793, 974, 1244, 1253, 1292, 1324, 1373
RsaNI GTAC 9 cut(s) 131, 659, 792, 973, 1243, 1252, 1291, 1323, 1372
RseI CAYNNNNRTG 1 cut(s) 1005
SacI GAGCTC 1 cut(s) 603
SaqAI TTAA 7 cut(s) 26, 477, 507, 549, 591, 626, 1238
SatI GCNGC 8 cut(s) 160, 338, 555, 672, 806, 827, 1179, 1182
Sau3AI GATC 3 cut(s) 850, 1057, 1276
Sau96I GGNCC 2 cut(s) 161, 1091
SchI GAGTC 1 cut(s) 662
ScrFI CCNGG 4 cut(s) 378, 699, 823, 1054
SduI GDGCHC 4 cut(s) 264, 603, 770, 1384
SfaNI GCATC 3 cut(s) 131, 809, 997
SfcI CTRYAG 1 cut(s) 1179
SmiMI CAYNNNNRTG 1 cut(s) 1005
SphI GCATGC 2 cut(s) 623, 920
Sse9I AATT 7 cut(s) 49, 83, 369, 468, 485, 1122, 1138
SsiI CCGC 5 cut(s) 159, 806, 1108, 1130, 1393
SspMI CTAG 2 cut(s) 198, 1377
SstI GAGCTC 1 cut(s) 603
StyD4I CCNGG 4 cut(s) 376, 697, 821, 1052
TaaI ACNGT 3 cut(s) 1098, 1251, 1322
TaiI ACGT 7 cut(s) 140, 360, 793, 799, 934, 1238, 1244
TaqI TCGA 3 cut(s) 864, 904, 1162
TasI AATT 7 cut(s) 49, 83, 369, 468, 485, 1122, 1138
TauI GCSGC 2 cut(s) 162, 808
TfiI GAWTC 2 cut(s) 941, 1151
Tru1I TTAA 7 cut(s) 26, 477, 507, 549, 591, 626, 1238
Tru9I TTAA 7 cut(s) 26, 477, 507, 549, 591, 626, 1238
TscAI CASTG 2 cut(s) 963, 1180
TseFI GTSAC 3 cut(s) 958, 1027, 1261
TseI GCWGC 6 cut(s) 337, 554, 671, 826, 1178, 1181
Tsp45I GTSAC 3 cut(s) 958, 1027, 1261
TspDTI ATGAA 4 cut(s) 125, 725, 765, 1242
TspGWI ACGGA 1 cut(s) 1246
TspRI CASTG 2 cut(s) 963, 1180
Tth111I GACNNNGTC 1 cut(s) 193
XapI RAATTY 1 cut(s) 485
XceI RCATGY 7 cut(s) 623, 875, 884, 911, 920, 997, 1193
XmnI GAANNNNTTC 1 cut(s) 732
XspI CTAG 2 cut(s) 198, 1377
Zsp2I ATGCAT 1 cut(s) 1012
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.