Prupe.1G215000_v2.0.a1

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000847
Physical Location & Seq
Reverse (-)
22795024 .. 22800359
5336 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G215000.1

Sequence Viewer

Length: 1407 bp
ATGGCAAAGAAACGGGGCAAGGCGTCAAAGGGAAAAGGCCTACCTTTAAGTCACGATGATGGGAGTAAAAAGATAGGGAAGAGTTTAACGAAGAAAAAAAAGAAATCTAGGAAGATAAGCGCATCTTCCATGCCTACACCTGCACCTGCACCTGTGCTTAACAGTGGCCCTTCTGCTTCCAATGTTGCTCATGCTACTTCATCTAGTCATGCTAATCCTACTATTAGGGAAAAGGTTCGGCCCGAAAAGAATGAGAAGGATAGTGGGCAATCACATTCTGGTTTTATATTCATGTGTAATGGAAAAACGAAACCTGAATGCTATCGATATCGTGTTTTTGGGCTACCCTTGGGAAAGGTAGAAGTTGTAAAGAAGATTACACCTGGTACAAAGCTATTTTTATATGACTTTGACTTGAAGCTCCTTTATGGCACATACAAGGCAACTTCAAATGGAGGATTGGATTTGGAGCCAATTGCGTTTAAGGGGAAATTTCCTGCACAGGTCAGATTTAAGATTTTCCAGGACTGTTTACCGCTTCCGGAAAATGCCTTCAAAGTAGCTATCAAAGACAACTATCAGGGAGGTTCCAAATTTAAGCAAGAGCTCAATAACAAACAGGTGCATGCTTTAATCTCATTGTTCCGCCCAATCACTGCATCTGTGCCTACTCCTGCTGCCCCTCTCCCAGATGTGGCTAGATCAGGCTCATTTCGATCTCCTATTACAGAAGAAGAATATCATCCAACAGCAAAACTGTGTCTTCAAAAGGGCTCCTATTTACCTGGCGTGCAATGTACTCATGAATTACCAATCTATATGCAATCCAAGCAGGTAGTCAGATATCCACTTTACGATAAATATGAGGCTGAAACACACGTGGCACATGCACAGCCTCCTACAGAACCTCGACATGTTGTTCAGCGTGCTTCTCTTCCTCATCATGCCGATTCCTATTATTTAGCAGCAGAACATGAGCCATACTTGCCTGAACAACCTTTCCTGTCTTATCAGGATGCATATAGAAGGTATGGGGTCACACTTGAGGATGAGATGGTTCCCAGGGATCAAGTTGTTACGTATAGAAGCGTGTATGACAGGTCACAGTTGCGGCAGGGAAGGGAGCGAGATGTTTTTGCCCATTCCGATTATGCTGCTGAATCGTATAGTCGAGAGTTGCCCACTGCTGCAGCCTCACGTGTTATGTTGCAATCACATTCTTTAGCACCATCTTATGAATGGCCATCAACTTACCAACCGTATTATCAAGTGACAGCCCATCAGGATCAGAGCTGGGTGTATGCTGATCCTCTTCAAAGACCATTGCATGGTAGCTCTAACTCAGTTGAGGGAAATGTGCCAATCTCTTCACGCTTTTCTTGCACCGGATCTGCCCTTTACCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

469

Amino Acids

52.37

Weight (kDa)

9.39

Isoelectric Point (pI)

48.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 148, 154
Acc36I ACCTGC 3 cut(s) 148, 154, 823
AccB7I CCANNNNNTGG 1 cut(s) 1328
AccIII TCCGGA 1 cut(s) 541
AciI CCGC 3 cut(s) 536, 646, 1111
AclWI GGATC 4 cut(s) 1074, 1293, 1301, 1396
AcoI YGGCCR 1 cut(s) 1241
AcsI RAATTY 2 cut(s) 491, 593
AcvI CACGTG 2 cut(s) 880, 1199
AcyI GRCGYC 1 cut(s) 23
AfaI GTAC 2 cut(s) 388, 799
AfiI CCNNNNNNNGG 2 cut(s) 694, 1328
AflIII ACRYGT 3 cut(s) 877, 913, 1198
AgsI TTSAA 5 cut(s) 418, 450, 556, 767, 1316
AjnI CCWGG 4 cut(s) 382, 522, 784, 1061
AloI GAACNNNNNNTCC 2 cut(s) 984, 1016
AluBI AGCT 6 cut(s) 394, 421, 563, 607, 1293, 1335
AluI AGCT 6 cut(s) 394, 421, 563, 607, 1293, 1335
Alw21I GWGCWC 1 cut(s) 609
AlwI GGATC 4 cut(s) 1074, 1293, 1301, 1396
Aor13HI TCCGGA 1 cut(s) 541
AoxI GGCC 4 cut(s) 37, 166, 239, 1241
ApeKI GCWGC 5 cut(s) 677, 965, 1154, 1187, 1190
ApoI RAATTY 2 cut(s) 491, 593
Asp700I GAANNNNTTC 1 cut(s) 234
AspLEI GCGC 1 cut(s) 122
AspS9I GGNCC 2 cut(s) 167, 240
BalI TGGCCA 1 cut(s) 1243
BanII GRGCYC 2 cut(s) 609, 776
BbrPI CACGTG 2 cut(s) 880, 1199
BbsI GAAGAC 1 cut(s) 755
Bbv12I GWGCWC 1 cut(s) 609
BbvI GCAGC 5 cut(s) 664, 977, 1141, 1174, 1202
BccI CCATC 5 cut(s) 53, 1048, 1237, 1252, 1287
BcgI CGANNNNNNTGC 2 cut(s) 1136, 1170
BciT130I CCWGG 4 cut(s) 384, 524, 786, 1063
BfaI CTAG 3 cut(s) 108, 204, 699
BfmI CTRYAG 2 cut(s) 900, 1188
BfuAI ACCTGC 3 cut(s) 148, 154, 823
BisI GCNGC 6 cut(s) 678, 966, 1112, 1155, 1188, 1191
BlsI GCNGC 6 cut(s) 679, 967, 1113, 1156, 1189, 1192
Bme1390I CCNGG 4 cut(s) 384, 524, 786, 1063
BmgT120I GGNCC 2 cut(s) 167, 240
BmiI GGNNCC 4 cut(s) 471, 589, 775, 1059
BmrFI CCNGG 4 cut(s) 384, 524, 786, 1063
BmsI GCATC 3 cut(s) 131, 668, 1006
BpiI GAAGAC 1 cut(s) 755
BpuEI CTTGAG 1 cut(s) 1064
Bsa29I ATCGAT 1 cut(s) 325
BsaAI YACGTR 3 cut(s) 880, 1080, 1199
BsaHI GRCGYC 1 cut(s) 23
BsaJI CCNNGG 3 cut(s) 348, 1061, 1062
BsaWI WCCGGW 2 cut(s) 541, 1385
Bsc4I CCNNNNNNNGG 2 cut(s) 694, 1328
Bse3DI GCAATG 2 cut(s) 800, 1322
BseAI TCCGGA 1 cut(s) 541
BseBI CCWGG 4 cut(s) 384, 524, 786, 1063
BseCI ATCGAT 1 cut(s) 325
BseDI CCNNGG 3 cut(s) 348, 1061, 1062
BseGI GGATG 3 cut(s) 742, 1021, 1054
BseLI CCNNNNNNNGG 2 cut(s) 694, 1328
BseMI GCAATG 2 cut(s) 800, 1322
BseMII CTCAG 1 cut(s) 1356
BseXI GCAGC 5 cut(s) 664, 977, 1141, 1174, 1202
BseYI CCCAGC 1 cut(s) 1293
BsgI GTGCAG 3 cut(s) 126, 132, 483
BshFI GGCC 4 cut(s) 39, 168, 241, 1243
BshVI ATCGAT 1 cut(s) 325
BsiHKAI GWGCWC 1 cut(s) 609
BsiSI CCGG 2 cut(s) 542, 1386
BslI CCNNNNNNNGG 2 cut(s) 694, 1328
BsmI GAATGC 1 cut(s) 323
BsnI GGCC 4 cut(s) 39, 168, 241, 1243
Bsp1286I GDGCHC 2 cut(s) 609, 776
Bsp13I TCCGGA 1 cut(s) 541
Bsp143I GATC 6 cut(s) 701, 716, 1066, 1285, 1306, 1388
BspACI CCGC 3 cut(s) 536, 646, 1111
BspANI GGCC 4 cut(s) 39, 168, 241, 1243
BspCNI CTCAG 1 cut(s) 1355
BspDI ATCGAT 1 cut(s) 325
BspEI TCCGGA 1 cut(s) 541
BspHI TCATGA 1 cut(s) 802
BspLI GGNNCC 4 cut(s) 471, 589, 775, 1059
BspMAI CTGCAG 1 cut(s) 1192
BspMI ACCTGC 3 cut(s) 148, 154, 823
BspPI GGATC 4 cut(s) 1074, 1293, 1301, 1396
BsrDI GCAATG 2 cut(s) 800, 1322
BssECI CCNNGG 3 cut(s) 348, 1061, 1062
BssMI GATC 6 cut(s) 701, 716, 1066, 1285, 1306, 1388
BssNI GRCGYC 1 cut(s) 23
BssT1I CCWWGG 1 cut(s) 348
Bst2UI CCWGG 4 cut(s) 384, 524, 786, 1063
Bst4CI ACNGT 5 cut(s) 164, 530, 759, 1107, 1260
Bst6I CTCTTC 4 cut(s) 74, 939, 1317, 1372
BstACI GRCGYC 1 cut(s) 23
BstBAI YACGTR 3 cut(s) 880, 1080, 1199
BstC8I GCNNGC 3 cut(s) 627, 791, 927
BstDEI CTNAG 1 cut(s) 1342
BstF5I GGATG 3 cut(s) 742, 1021, 1054
BstHHI GCGC 1 cut(s) 122
BstKTI GATC 6 cut(s) 704, 719, 1069, 1288, 1309, 1391
BstMBI GATC 6 cut(s) 701, 716, 1066, 1285, 1306, 1388
BstMWI GCNNNNNNNGC 3 cut(s) 829, 985, 1380
BstNI CCWGG 4 cut(s) 384, 524, 786, 1063
BstNSI RCATGY 3 cut(s) 629, 890, 917
BstSCI CCNGG 4 cut(s) 382, 522, 784, 1061
BstSFI CTRYAG 2 cut(s) 900, 1188
BstSNI TACGTA 1 cut(s) 1080
BstV1I GCAGC 5 cut(s) 664, 977, 1141, 1174, 1202
BstV2I GAAGAC 1 cut(s) 755
BstX2I RGATCY 1 cut(s) 1388
BstYI RGATCY 1 cut(s) 1388
Bsu15I ATCGAT 1 cut(s) 325
BsuRI GGCC 4 cut(s) 39, 168, 241, 1243
BsuTUI ATCGAT 1 cut(s) 325
BtsCI GGATG 3 cut(s) 742, 1021, 1054
BtsI GCAGTG 2 cut(s) 654, 1182
BtsIMutI CAGTG 4 cut(s) 169, 654, 1182, 1402
BveI ACCTGC 3 cut(s) 148, 154, 823
Cac8I GCNNGC 3 cut(s) 627, 791, 927
CciI TCATGA 1 cut(s) 802
CfoI GCGC 1 cut(s) 122
Cfr13I GGNCC 2 cut(s) 167, 240
ClaI ATCGAT 1 cut(s) 325
CseI GACGC 1 cut(s) 12
CsiI ACCWGGT 1 cut(s) 382
Csp6I GTAC 2 cut(s) 387, 798
CviQI GTAC 2 cut(s) 387, 798
DdeI CTNAG 1 cut(s) 1342
DpnI GATC 6 cut(s) 703, 718, 1068, 1287, 1308, 1390
DpnII GATC 6 cut(s) 701, 716, 1066, 1285, 1306, 1388
EaeI YGGCCR 1 cut(s) 1241
Eam1104I CTCTTC 4 cut(s) 74, 939, 1317, 1372
EarI CTCTTC 4 cut(s) 74, 939, 1317, 1372
EciI GGCGGA 1 cut(s) 635
Ecl136II GAGCTC 1 cut(s) 607
Eco105I TACGTA 1 cut(s) 1080
Eco130I CCWWGG 1 cut(s) 348
Eco147I AGGCCT 1 cut(s) 39
Eco24I GRGCYC 2 cut(s) 609, 776
Eco32I GATATC 2 cut(s) 329, 845
Eco53kI GAGCTC 1 cut(s) 607
Eco72I CACGTG 2 cut(s) 880, 1199
EcoICRI GAGCTC 1 cut(s) 607
EcoRII CCWGG 4 cut(s) 382, 522, 784, 1061
EcoRV GATATC 2 cut(s) 329, 845
EcoT14I CCWWGG 1 cut(s) 348
EcoT22I ATGCAT 1 cut(s) 1021
EcoT38I GRGCYC 2 cut(s) 609, 776
ErhI CCWWGG 1 cut(s) 348
FalI AAGNNNNNCTT 2 cut(s) 109, 141
Fnu4HI GCNGC 6 cut(s) 678, 966, 1112, 1155, 1188, 1191
FokI GGATG 3 cut(s) 729, 1028, 1061
FriOI GRGCYC 2 cut(s) 609, 776
Fsp4HI GCNGC 6 cut(s) 678, 966, 1112, 1155, 1188, 1191
FspBI CTAG 3 cut(s) 108, 204, 699
GlaI GCGC 1 cut(s) 121
GluI GCNGC 6 cut(s) 678, 966, 1112, 1155, 1188, 1191
GsaI CCCAGC 1 cut(s) 1297
HaeIII GGCC 4 cut(s) 39, 168, 241, 1243
HapII CCGG 2 cut(s) 542, 1386
HgaI GACGC 1 cut(s) 12
HhaI GCGC 1 cut(s) 122
Hin1I GRCGYC 1 cut(s) 23
Hin6I GCGC 1 cut(s) 120
HinP1I GCGC 1 cut(s) 120
HinfI GANTC 2 cut(s) 950, 1160
HpaII CCGG 2 cut(s) 542, 1386
Hpy166II GTNNAC 1 cut(s) 533
Hpy188I TCNGA 4 cut(s) 509, 842, 1147, 1290
Hpy188III TCNNGA 6 cut(s) 53, 542, 803, 1013, 1172, 1283
Hpy8I GTNNAC 1 cut(s) 533
HpyAV CCTTC 5 cut(s) 180, 250, 562, 1020, 1113
HpyCH4III ACNGT 5 cut(s) 164, 530, 759, 1107, 1260
HpyCH4IV ACGT 3 cut(s) 879, 1079, 1198
HpyF10VI GCNNNNNNNGC 3 cut(s) 829, 985, 1380
HpyF3I CTNAG 1 cut(s) 1342
HpySE526I ACGT 3 cut(s) 879, 1079, 1198
Hsp92I GRCGYC 1 cut(s) 23
HspAI GCGC 1 cut(s) 120
Kpn2I TCCGGA 1 cut(s) 541
Kzo9I GATC 6 cut(s) 701, 716, 1066, 1285, 1306, 1388
LmnI GCTCC 4 cut(s) 426, 469, 779, 1123
Lsp1109I GCAGC 5 cut(s) 664, 977, 1141, 1174, 1202
LweI GCATC 3 cut(s) 131, 668, 1006
MabI ACCWGGT 1 cut(s) 382
MaeI CTAG 3 cut(s) 108, 204, 699
MaeII ACGT 3 cut(s) 879, 1079, 1198
MaeIII GTNAC 5 cut(s) 50, 1036, 1075, 1101, 1270
MalI GATC 6 cut(s) 703, 718, 1068, 1287, 1308, 1390
MboI GATC 6 cut(s) 701, 716, 1066, 1285, 1306, 1388
MfeI CAATTG 1 cut(s) 474
MflI RGATCY 1 cut(s) 1388
MhlI GDGCHC 2 cut(s) 609, 776
MlsI TGGCCA 1 cut(s) 1243
MluCI AATT 4 cut(s) 474, 491, 593, 806
MluNI TGGCCA 1 cut(s) 1243
MmeI TCCRAC 1 cut(s) 770
Mox20I TGGCCA 1 cut(s) 1243
Mph1103I ATGCAT 1 cut(s) 1021
MroI TCCGGA 1 cut(s) 541
MroXI GAANNNNTTC 1 cut(s) 234
MscI TGGCCA 1 cut(s) 1243
MseI TTAA 7 cut(s) 47, 86, 159, 483, 513, 597, 632
MslI CAYNNNNRTG 1 cut(s) 57
Msp20I TGGCCA 1 cut(s) 1243
MspI CCGG 2 cut(s) 542, 1386
MspR9I CCNGG 4 cut(s) 384, 524, 786, 1063
MunI CAATTG 1 cut(s) 474
Mva1269I GAATGC 1 cut(s) 323
MvaI CCWGG 4 cut(s) 384, 524, 786, 1063
MwoI GCNNNNNNNGC 3 cut(s) 829, 985, 1380
NdeII GATC 6 cut(s) 701, 716, 1066, 1285, 1306, 1388
NlaIV GGNNCC 4 cut(s) 471, 589, 775, 1059
NmuCI GTSAC 4 cut(s) 50, 1036, 1101, 1270
NsiI ATGCAT 1 cut(s) 1021
NspI RCATGY 3 cut(s) 629, 890, 917
PaeI GCATGC 1 cut(s) 629
PagI TCATGA 1 cut(s) 802
PaqCI CACCTGC 2 cut(s) 148, 154
PasI CCCWGGG 1 cut(s) 1062
PceI AGGCCT 1 cut(s) 39
PciI ACATGT 1 cut(s) 913
PctI GAATGC 1 cut(s) 323
PdmI GAANNNNTTC 1 cut(s) 234
PfeI GAWTC 2 cut(s) 950, 1160
PflMI CCANNNNNTGG 1 cut(s) 1328
PfoI TCCNGGA 1 cut(s) 522
PkrI GCNGC 6 cut(s) 679, 967, 1113, 1156, 1189, 1192
PmaCI CACGTG 2 cut(s) 880, 1199
PmlI CACGTG 2 cut(s) 880, 1199
Ppu21I YACGTR 3 cut(s) 880, 1080, 1199
PscI ACATGT 1 cut(s) 913
Psp124BI GAGCTC 1 cut(s) 609
Psp6I CCWGG 4 cut(s) 382, 522, 784, 1061
PspCI CACGTG 2 cut(s) 880, 1199
PspFI CCCAGC 1 cut(s) 1293
PspGI CCWGG 4 cut(s) 382, 522, 784, 1061
PspN4I GGNNCC 4 cut(s) 471, 589, 775, 1059
PspPI GGNCC 2 cut(s) 167, 240
PstI CTGCAG 1 cut(s) 1192
PsuI RGATCY 1 cut(s) 1388
RsaI GTAC 2 cut(s) 388, 799
RsaNI GTAC 2 cut(s) 387, 798
RseI CAYNNNNRTG 1 cut(s) 57
SacI GAGCTC 1 cut(s) 609
SaqAI TTAA 7 cut(s) 47, 86, 159, 483, 513, 597, 632
SatI GCNGC 6 cut(s) 678, 966, 1112, 1155, 1188, 1191
Sau3AI GATC 6 cut(s) 701, 716, 1066, 1285, 1306, 1388
Sau96I GGNCC 2 cut(s) 167, 240
ScrFI CCNGG 4 cut(s) 384, 524, 786, 1063
SduI GDGCHC 2 cut(s) 609, 776
SexAI ACCWGGT 1 cut(s) 382
SfaNI GCATC 3 cut(s) 131, 668, 1006
SfcI CTRYAG 2 cut(s) 900, 1188
SmiMI CAYNNNNRTG 1 cut(s) 57
SmlI CTYRAG 1 cut(s) 1043
SmoI CTYRAG 1 cut(s) 1043
SnaBI TACGTA 1 cut(s) 1080
SphI GCATGC 1 cut(s) 629
Sse9I AATT 4 cut(s) 474, 491, 593, 806
SseBI AGGCCT 1 cut(s) 39
SsiI CCGC 3 cut(s) 536, 646, 1111
SspMI CTAG 3 cut(s) 108, 204, 699
SstI GAGCTC 1 cut(s) 609
StuI AGGCCT 1 cut(s) 39
StyD4I CCNGG 4 cut(s) 382, 522, 784, 1061
StyI CCWWGG 1 cut(s) 348
TaaI ACNGT 5 cut(s) 164, 530, 759, 1107, 1260
TaiI ACGT 3 cut(s) 882, 1082, 1201
TaqI TCGA 4 cut(s) 325, 715, 910, 1171
TasI AATT 4 cut(s) 474, 491, 593, 806
TatI WGTACW 1 cut(s) 797
TauI GCSGC 1 cut(s) 1114
TfiI GAWTC 2 cut(s) 950, 1160
Tru1I TTAA 7 cut(s) 47, 86, 159, 483, 513, 597, 632
Tru9I TTAA 7 cut(s) 47, 86, 159, 483, 513, 597, 632
TscAI CASTG 3 cut(s) 169, 661, 1189
TseFI GTSAC 4 cut(s) 50, 1036, 1101, 1270
TseI GCWGC 5 cut(s) 677, 965, 1154, 1187, 1190
Tsp45I GTSAC 4 cut(s) 50, 1036, 1101, 1270
TspDTI ATGAA 4 cut(s) 189, 280, 819, 1251
TspRI CASTG 3 cut(s) 169, 661, 1189
Van91I CCANNNNNTGG 1 cut(s) 1328
XapI RAATTY 2 cut(s) 491, 593
XceI RCATGY 3 cut(s) 629, 890, 917
XcmI CCANNNNNNNNNTGG 1 cut(s) 1236
XmnI GAANNNNTTC 1 cut(s) 234
XspI CTAG 3 cut(s) 108, 204, 699
Zsp2I ATGCAT 1 cut(s) 1021
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.