MD14G1147400.v1.1

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000847
Physical Location & Seq
Reverse (-)
24020720 .. 24024183
3464 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1147400.v1.1.491

Sequence Viewer

Length: 1446 bp
ATGACACGTAACGTAGGGCTTCACCTTCTCCTGCCACGCGCAAATTTTCCGACTCCTAAATATTTCCAATTCTCCAGAGACGCTCCAAGTTTCCTCTCCTTTGCATTTGTCTTCAACATGACTGAGAGAAGATATGACGGATTTGAAAACAAGGGGCCGATTGTATACCACACGAGTGCCAAGTATTACCCTGCTCCTGCCCCTTCAAATTATGCGTATGACCATGTTTCTGCTTCTGCTTGTGCTAACGAGGATCATGGGGTGCATAAGGACCCTCTGGGTCTGGAAGACTTTTCCGGGTATATATTTATGTGCAATGGAAGAACAAAACCGGAATGCTACCTATACCGTGTGTTTGGACTTCCTGCTGGAAGAAGGGAAGTTATAGAGAGGATCCGGCCAGGCACAAAGCTGTTTCTGTTTGATTATGATGTCAAGTTTCTTTATGGGGTGTATGAAGCAACCACTGCTGGAAAGATGAACTTGGAAAGAACGGCTTTTGGTGGAAGCTTTCCCGCGCAGGTAAGATTCAGAATTTACAAGGAATGTCTCCCCTTGCCTGAGAGTGATTTTAAACATGCTATTCTAGATAACTACCAGAAAGGCTCCAAGAAGTTTAACCCCATTCTCAATTGCCAACAAGTAAGCCATTTGTTATCACTGTTTCATCCTCTAACCACACAATCAACTGTCAAATATGACAATTCCTATATAAAGGATCAATACCCAAGGCTGCCTCCTTCAGGCGCTACATACTATAACTCAATGCGTCTCAGTCAAGCCCCACAAGTGTTGGTTCCTCAATATGTTCCACAGGCAGTCCTAGTTCAGCAGCGGGATGGTCGTGGATCCACAGGAAACACGGGTCTTTTCCATCATGCAGACCAACCTGCTGTGCCAAATGCAGGTTCACAATCAGCAATGCCAACCCAATCTACGGAGGAACAGTTTCAGTCCCCAGCAAGCCTGCCTTCCATGAAGGATCCAACTGCATCTTTGATATATGGCAGTCTTACTTCACCAATGTTGGAGTCTCAGCCTCAGTATATTCAACCGAGTGTCGTACACCAACAGCCTGCTAGCTATGGATACATGTCATACATGGGCTACATTTATCCTGCTGTGCAACAGCAAGCTATGCCAGCTCCAAATCAGACATATTATTCTGCACAAGTGGCGCACAGTTACTCGGCTGAACTCCCTGCCTCGCAAGCACCCACATCATATGAAAGCTATCACAAGCACAAGGCCACACATGATGAAGTTCCTACTGATCAACAAACGAGCTTGGGATATGGGTACAATCAGTTACCTTTGAAGACGGAGACTGGAACTAATTTACAGCAGGGGAATGCTGCCGAATACTACATCTACCAAGTGCCTACAGTCCAGTATGTTTCATCACCTACACAAACCACACACCTCAACAACATTGGAAGCACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

482

Amino Acids

53.94

Weight (kDa)

7.68

Isoelectric Point (pI)

54.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 100 - 222 2.8e-47 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 511, 896, 898
AccI GTMKAC 1 cut(s) 165
AccII CGCG 2 cut(s) 39, 518
AciI CCGC 2 cut(s) 516, 835
AclWI GGATC 8 cut(s) 261, 388, 401, 726, 843, 856, 977, 990
AcoI YGGCCR 1 cut(s) 398
AcsI RAATTY 2 cut(s) 43, 534
AcuI CTGAAG 1 cut(s) 726
AfaI GTAC 2 cut(s) 1065, 1301
AfiI CCNNNNNNNGG 3 cut(s) 743, 905, 937
AflIII ACRYGT 2 cut(s) 5, 1092
AgsI TTSAA 5 cut(s) 115, 146, 207, 1052, 1318
AjnI CCWGG 1 cut(s) 400
AjuI GAANNNNNNNTTGG 2 cut(s) 892, 924
AleI CACNNNNGTG 1 cut(s) 174
AluBI AGCT 7 cut(s) 412, 510, 1083, 1136, 1145, 1233, 1287
AluI AGCT 7 cut(s) 412, 510, 1083, 1136, 1145, 1233, 1287
Alw26I GTCTC 5 cut(s) 72, 554, 776, 1038, 1319
AlwI GGATC 8 cut(s) 261, 388, 401, 726, 843, 856, 977, 990
AoxI GGCC 3 cut(s) 155, 398, 1248
ApeKI GCWGC 3 cut(s) 733, 832, 1355
ApoI RAATTY 2 cut(s) 43, 534
Asp700I GAANNNNTTC 1 cut(s) 948
AspLEI GCGC 4 cut(s) 41, 520, 749, 1180
AspS9I GGNCC 2 cut(s) 155, 271
AsuC2I CCSGG 1 cut(s) 298
AsuHPI GGTGA 3 cut(s) 14, 1011, 1395
AsuNHI GCTAGC 1 cut(s) 1079
AvaII GGWCC 1 cut(s) 271
BamHI GGATCC 3 cut(s) 393, 848, 982
BauI CACGAG 1 cut(s) 172
BbsI GAAGAC 3 cut(s) 103, 294, 1325
BbvI GCAGC 3 cut(s) 720, 844, 1342
BccI CCATC 2 cut(s) 833, 882
BceAI ACGGC 1 cut(s) 510
BciT130I CCWGG 1 cut(s) 402
BciVI GTATCC 1 cut(s) 1082
BclI TGATCA 1 cut(s) 1273
BcnI CCSGG 1 cut(s) 298
BcoDI GTCTC 5 cut(s) 72, 554, 776, 1038, 1319
BfaI CTAG 3 cut(s) 587, 824, 1080
BfmI CTRYAG 1 cut(s) 1383
BfoI RGCGCY 1 cut(s) 750
BfuAI ACCTGC 3 cut(s) 511, 896, 898
BfuI GTATCC 1 cut(s) 1082
BisI GCNGC 3 cut(s) 734, 833, 1356
BlsI GCNGC 3 cut(s) 735, 834, 1357
Bme1390I CCNGG 2 cut(s) 298, 402
Bme18I GGWCC 1 cut(s) 271
BmgT120I GGNCC 2 cut(s) 155, 271
BmiI GGNNCC 7 cut(s) 156, 273, 395, 607, 798, 850, 984
BmrFI CCNGG 2 cut(s) 298, 402
BmsI GCATC 1 cut(s) 1001
BmtI GCTAGC 1 cut(s) 1083
BpiI GAAGAC 3 cut(s) 103, 294, 1325
BpmI CTGGAG 1 cut(s) 58
BpuMI CCSGG 1 cut(s) 298
BsaAI YACGTR 1 cut(s) 8
BsaJI CCNNGG 1 cut(s) 728
BsaWI WCCGGW 1 cut(s) 331
BsaXI ACNNNNNCTCC 2 cut(s) 932, 962
Bsc4I CCNNNNNNNGG 3 cut(s) 743, 905, 937
Bse1I ACTGG 2 cut(s) 1333, 1390
Bse3DI GCAATG 2 cut(s) 322, 927
BseBI CCWGG 1 cut(s) 402
BseDI CCNNGG 1 cut(s) 728
BseGI GGATG 2 cut(s) 667, 844
BseLI CCNNNNNNNGG 3 cut(s) 743, 905, 937
BseMI GCAATG 2 cut(s) 322, 927
BseMII CTCAG 5 cut(s) 114, 552, 787, 1049, 1055
BseNI ACTGG 2 cut(s) 1333, 1390
BseXI GCAGC 3 cut(s) 720, 844, 1342
BseYI CCCAGC 1 cut(s) 958
BsgI GTGCAG 1 cut(s) 1152
Bsh1236I CGCG 2 cut(s) 39, 518
BshFI GGCC 3 cut(s) 157, 400, 1250
BsiSI CCGG 3 cut(s) 297, 332, 397
BslFI GGGAC 1 cut(s) 940
BslI CCNNNNNNNGG 3 cut(s) 743, 905, 937
BsmAI GTCTC 5 cut(s) 72, 554, 776, 1038, 1319
BsmBI CGTCTC 2 cut(s) 72, 776
BsmFI GGGAC 1 cut(s) 940
BsmI GAATGC 2 cut(s) 341, 1357
BsnI GGCC 3 cut(s) 157, 400, 1250
Bsp143I GATC 6 cut(s) 253, 393, 718, 848, 982, 1273
BspACI CCGC 2 cut(s) 516, 835
BspANI GGCC 3 cut(s) 157, 400, 1250
BspCNI CTCAG 5 cut(s) 115, 553, 786, 1048, 1054
BspFNI CGCG 2 cut(s) 39, 518
BspLI GGNNCC 7 cut(s) 156, 273, 395, 607, 798, 850, 984
BspMI ACCTGC 3 cut(s) 511, 896, 898
BspOI GCTAGC 1 cut(s) 1083
BspPI GGATC 8 cut(s) 261, 388, 401, 726, 843, 856, 977, 990
BsrDI GCAATG 2 cut(s) 322, 927
BsrI ACTGG 2 cut(s) 1333, 1390
BssECI CCNNGG 1 cut(s) 728
BssMI GATC 6 cut(s) 253, 393, 718, 848, 982, 1273
BssNAI GTATAC 1 cut(s) 166
BssSI CACGAG 1 cut(s) 172
BssT1I CCWWGG 1 cut(s) 728
Bst1107I GTATAC 1 cut(s) 166
Bst2BI CACGAG 1 cut(s) 172
Bst2UI CCWGG 1 cut(s) 402
Bst4CI ACNGT 6 cut(s) 350, 663, 691, 948, 1184, 1387
BstAPI GCANNNNNTGC 2 cut(s) 467, 1138
BstBAI YACGTR 1 cut(s) 8
BstC8I GCNNGC 7 cut(s) 964, 968, 1077, 1081, 1134, 1143, 1212
BstDEI CTNAG 5 cut(s) 123, 561, 773, 1035, 1041
BstENI CCTNNNNNAGG 1 cut(s) 741
BstF5I GGATG 2 cut(s) 667, 844
BstFNI CGCG 2 cut(s) 39, 518
BstH2I RGCGCY 1 cut(s) 750
BstHHI GCGC 4 cut(s) 41, 520, 749, 1180
BstKTI GATC 6 cut(s) 256, 396, 721, 851, 985, 1276
BstMAI GTCTC 5 cut(s) 72, 554, 776, 1038, 1319
BstMBI GATC 6 cut(s) 253, 393, 718, 848, 982, 1273
BstMWI GCNNNNNNNGC 5 cut(s) 467, 1138, 1142, 1175, 1211
BstNI CCWGG 1 cut(s) 402
BstNSI RCATGY 2 cut(s) 581, 1096
BstSCI CCNGG 2 cut(s) 296, 400
BstSFI CTRYAG 1 cut(s) 1383
BstUI CGCG 2 cut(s) 39, 518
BstV1I GCAGC 3 cut(s) 720, 844, 1342
BstV2I GAAGAC 3 cut(s) 103, 294, 1325
BstX2I RGATCY 3 cut(s) 393, 848, 982
BstYI RGATCY 3 cut(s) 393, 848, 982
BstZ17I GTATAC 1 cut(s) 166
BsuI GTATCC 1 cut(s) 1082
BsuRI GGCC 3 cut(s) 157, 400, 1250
BtsCI GGATG 2 cut(s) 667, 844
BtsI GCAGTG 1 cut(s) 465
BtsIMutI CAGTG 2 cut(s) 465, 659
BveI ACCTGC 3 cut(s) 511, 896, 898
Cac8I GCNNGC 7 cut(s) 964, 968, 1077, 1081, 1134, 1143, 1212
CfoI GCGC 4 cut(s) 41, 520, 749, 1180
Cfr13I GGNCC 2 cut(s) 155, 271
CseI GACGC 2 cut(s) 89, 758
Csp6I GTAC 2 cut(s) 1064, 1300
CspCI CAANNNNNGTGG 2 cut(s) 774, 809
CviAII CATG 9 cut(s) 118, 224, 257, 578, 878, 976, 1093, 1102, 1256
CviQI GTAC 2 cut(s) 1064, 1300
DdeI CTNAG 5 cut(s) 123, 561, 773, 1035, 1041
DpnI GATC 6 cut(s) 255, 395, 720, 850, 984, 1275
DpnII GATC 6 cut(s) 253, 393, 718, 848, 982, 1273
DraI TTTAAA 1 cut(s) 574
EaeI YGGCCR 1 cut(s) 398
Eco130I CCWWGG 1 cut(s) 728
Eco47I GGWCC 1 cut(s) 271
Eco57I CTGAAG 1 cut(s) 726
EcoNI CCTNNNNNAGG 1 cut(s) 741
EcoO109I RGGNCCY 1 cut(s) 271
EcoRII CCWGG 1 cut(s) 400
EcoT14I CCWWGG 1 cut(s) 728
ErhI CCWWGG 1 cut(s) 728
Esp3I CGTCTC 2 cut(s) 72, 776
FaeI CATG 9 cut(s) 121, 227, 260, 581, 881, 979, 1096, 1105, 1259
FalI AAGNNNNNCTT 6 cut(s) 467, 499, 481, 513, 955, 987
FaqI GGGAC 1 cut(s) 940
FatI CATG 9 cut(s) 117, 223, 256, 577, 877, 975, 1092, 1101, 1255
FauI CCCGC 2 cut(s) 523, 828
FauNDI CATATG 1 cut(s) 1225
FbaI TGATCA 1 cut(s) 1273
FblI GTMKAC 1 cut(s) 165
Fnu4HI GCNGC 3 cut(s) 734, 833, 1356
FokI GGATG 2 cut(s) 654, 851
Fsp4HI GCNGC 3 cut(s) 734, 833, 1356
FspBI CTAG 3 cut(s) 587, 824, 1080
GlaI GCGC 4 cut(s) 40, 519, 748, 1179
GluI GCNGC 3 cut(s) 734, 833, 1356
GsaI CCCAGC 1 cut(s) 962
GsuI CTGGAG 1 cut(s) 58
HaeII RGCGCY 1 cut(s) 750
HaeIII GGCC 3 cut(s) 157, 400, 1250
HapII CCGG 3 cut(s) 297, 332, 397
HgaI GACGC 2 cut(s) 89, 758
HhaI GCGC 4 cut(s) 41, 520, 749, 1180
Hin1II CATG 9 cut(s) 121, 227, 260, 581, 881, 979, 1096, 1105, 1259
Hin6I GCGC 4 cut(s) 39, 518, 747, 1178
HinP1I GCGC 4 cut(s) 39, 518, 747, 1178
HindIII AAGCTT 1 cut(s) 508
HinfI GANTC 3 cut(s) 52, 528, 1031
HpaII CCGG 3 cut(s) 297, 332, 397
HphI GGTGA 3 cut(s) 14, 1011, 1395
Hpy166II GTNNAC 3 cut(s) 166, 911, 1066
Hpy188I TCNGA 3 cut(s) 51, 533, 1155
Hpy188III TCNNGA 3 cut(s) 75, 284, 587
Hpy8I GTNNAC 3 cut(s) 166, 911, 1066
HpyAV CCTTC 6 cut(s) 35, 213, 369, 750, 973, 981
HpyCH4III ACNGT 6 cut(s) 350, 663, 691, 948, 1184, 1387
HpyCH4IV ACGT 2 cut(s) 7, 12
HpyCH4V TGCA 8 cut(s) 104, 265, 315, 881, 905, 992, 1126, 1169
HpyF10VI GCNNNNNNNGC 5 cut(s) 467, 1138, 1142, 1175, 1211
HpyF3I CTNAG 5 cut(s) 123, 561, 773, 1035, 1041
HpySE526I ACGT 2 cut(s) 7, 12
Hsp92II CATG 9 cut(s) 121, 227, 260, 581, 881, 979, 1096, 1105, 1259
HspAI GCGC 4 cut(s) 39, 518, 747, 1178
Ksp22I TGATCA 1 cut(s) 1273
Kzo9I GATC 6 cut(s) 253, 393, 718, 848, 982, 1273
LmnI GCTCC 4 cut(s) 88, 199, 611, 1150
Lsp1109I GCAGC 3 cut(s) 720, 844, 1342
LweI GCATC 1 cut(s) 1001
MaeI CTAG 3 cut(s) 587, 824, 1080
MaeII ACGT 2 cut(s) 7, 12
MaeIII GTNAC 3 cut(s) 8, 1184, 1308
MalI GATC 6 cut(s) 255, 395, 720, 850, 984, 1275
MboI GATC 6 cut(s) 253, 393, 718, 848, 982, 1273
MboII GAAGA 6 cut(s) 103, 141, 299, 333, 384, 1330
MfeI CAATTG 1 cut(s) 631
MflI RGATCY 3 cut(s) 393, 848, 982
MluCI AATT 7 cut(s) 43, 68, 208, 534, 631, 703, 1336
MlyI GAGTC 2 cut(s) 46, 1040
MmeI TCCRAC 3 cut(s) 74, 1008, 1010
MroXI GAANNNNTTC 1 cut(s) 948
MseI TTAA 2 cut(s) 573, 618
MslI CAYNNNNRTG 1 cut(s) 174
MspA1I CMGCKG 1 cut(s) 835
MspI CCGG 3 cut(s) 297, 332, 397
MspR9I CCNGG 2 cut(s) 298, 402
MunI CAATTG 1 cut(s) 631
Mva1269I GAATGC 2 cut(s) 341, 1357
MvaI CCWGG 1 cut(s) 402
MvnI CGCG 2 cut(s) 39, 518
MwoI GCNNNNNNNGC 5 cut(s) 467, 1138, 1142, 1175, 1211
NciI CCSGG 1 cut(s) 298
NdeI CATATG 1 cut(s) 1225
NdeII GATC 6 cut(s) 253, 393, 718, 848, 982, 1273
NheI GCTAGC 1 cut(s) 1079
NlaIII CATG 9 cut(s) 121, 227, 260, 581, 881, 979, 1096, 1105, 1259
NlaIV GGNNCC 7 cut(s) 156, 273, 395, 607, 798, 850, 984
NmeAIII GCCGAG 1 cut(s) 1169
NspI RCATGY 2 cut(s) 581, 1096
OliI CACNNNNGTG 1 cut(s) 174
PciI ACATGT 1 cut(s) 1092
PctI GAATGC 2 cut(s) 341, 1357
PdmI GAANNNNTTC 1 cut(s) 948
PfeI GAWTC 1 cut(s) 528
PkrI GCNGC 3 cut(s) 735, 834, 1357
PleI GAGTC 2 cut(s) 46, 1039
PpsI GAGTC 2 cut(s) 46, 1039
Ppu21I YACGTR 1 cut(s) 8
PpuMI RGGWCCY 1 cut(s) 271
PscI ACATGT 1 cut(s) 1092
Psp5II RGGWCCY 1 cut(s) 271
Psp6I CCWGG 1 cut(s) 400
PspFI CCCAGC 1 cut(s) 958
PspGI CCWGG 1 cut(s) 400
PspN4I GGNNCC 7 cut(s) 156, 273, 395, 607, 798, 850, 984
PspPI GGNCC 2 cut(s) 155, 271
PspPPI RGGWCCY 1 cut(s) 271
PsuI RGATCY 3 cut(s) 393, 848, 982
RsaI GTAC 2 cut(s) 1065, 1301
RsaNI GTAC 2 cut(s) 1064, 1300
RseI CAYNNNNRTG 1 cut(s) 174
SaqAI TTAA 2 cut(s) 573, 618
SatI GCNGC 3 cut(s) 734, 833, 1356
Sau3AI GATC 6 cut(s) 253, 393, 718, 848, 982, 1273
Sau96I GGNCC 2 cut(s) 155, 271
SchI GAGTC 2 cut(s) 46, 1040
ScrFI CCNGG 2 cut(s) 298, 402
SfaNI GCATC 1 cut(s) 1001
SfcI CTRYAG 1 cut(s) 1383
SinI GGWCC 1 cut(s) 271
SmiMI CAYNNNNRTG 1 cut(s) 174
Sse9I AATT 7 cut(s) 43, 68, 208, 534, 631, 703, 1336
SsiI CCGC 2 cut(s) 516, 835
SspI AATATT 1 cut(s) 62
SspMI CTAG 3 cut(s) 587, 824, 1080
StyD4I CCNGG 2 cut(s) 296, 400
StyI CCWWGG 1 cut(s) 728
TaaI ACNGT 6 cut(s) 350, 663, 691, 948, 1184, 1387
TaiI ACGT 2 cut(s) 10, 15
TasI AATT 7 cut(s) 43, 68, 208, 534, 631, 703, 1336
TfiI GAWTC 1 cut(s) 528
Tru1I TTAA 2 cut(s) 573, 618
Tru9I TTAA 2 cut(s) 573, 618
TscAI CASTG 2 cut(s) 472, 666
TseI GCWGC 3 cut(s) 733, 832, 1355
TspDTI ATGAA 7 cut(s) 471, 494, 656, 992, 1242, 1275, 1389
TspGWI ACGGA 3 cut(s) 153, 953, 1337
TspRI CASTG 2 cut(s) 472, 666
VpaK11BI GGWCC 1 cut(s) 271
XagI CCTNNNNNAGG 1 cut(s) 741
XapI RAATTY 2 cut(s) 43, 534
XbaI TCTAGA 1 cut(s) 586
XceI RCATGY 2 cut(s) 581, 1096
XmiI GTMKAC 1 cut(s) 165
XmnI GAANNNNTTC 1 cut(s) 948
XspI CTAG 3 cut(s) 587, 824, 1080
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.