Rw4G024870

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000847
Physical Location & Seq
Reverse (-)
50795324 .. 50799391
4068 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G024870.1

Sequence Viewer

Length: 1338 bp
ATGGGAAAAAGACGAGGCAAGCGTCTAAGCAAAAGAGGCCTAACTAGAGATCATGATGGTAGGATTAGGAGGATAGACAAGAGTTTTACAAAGGAAAAGAAGAAATCTAAGAAGATAACCGAATCTTCCTTGCCTCAGACTGCAGCTGAGCTTAGTGGTAGTACTAGTGCCAATTCTGCTTCCAATAAAGCAACTTCTTCCAGTCATGCTAATCAACCTACTAGAGAAAGGCTTCGATCAAAAAACAAAGGCAAGGATAGTGGGCAATCACATTCTGGTTTTATCTTTATGTGTAATGGAAAGACTAAACCCGAATGTTATCAGTATCGCGTTTTTGGGCTACCAATGGCAAAAGTAGAAGTTGTGAAGACAATCAAACAGGGTACACATCTATTTTTATATGACTTTGACTTGAAGCTCCTTTATGGCACATACAGCGCAACTTCGGATGGAGCATTAGATTTGGAGCCAATTGCGTTCAAGGGAAAATTTCCTGCACAGGTCAGGTTTAAGATTTCCAAGGATTGTTTACCACTTCACGAAAGTTCTTTTAAAACAGCCATAAGAGACAATTACCAGGGCGGTGGTAAGTTTAAACAAGAGCTGAATAACAAACAGGTGCGTGCTCTAATCTCATTGTTCCGCCCAATCACTGCATCAATGCCTACTTCTGCTGCCCATCACCGAGATGTGGCAAGAACAGGCTCATATCCATCTCCTATTAGGGAAGAAGGATTTCATCCAACAGCAAGGCCGTCTCTTCAAGAGGGCTTATATTTATCTAGCGTACAACATGCTCATGCATTGCCACTTCTTAATACGCAATCCATACAGGTTGTTCCCTATCCACGTTTTAAAAAATATGAAGCAGAAAGATGTGTGGCACGTGTTCAGCCTCCCATAGAGCCTCGACATGTTGTTCAGCATCAGCCAGATTCATATTATCTAGCAGCGGCCCATGAGCCATACGTGCCTGAAAAACCTTTCTTGTCCTCCCATGATGCATATAGAAGGTATTTTCCTCTGCCACAACTTTGTACAATTAAGTACCCTTTGAGCCTCTATTATGTGGTCTTCTGTAATGTGGCAGTTTCTTTTATCTTCTATACTACTACCAAGATCTATATTAAAATGGAGAACTATGCAACCAACAATCACCTTCTGTCTTTGTCCTCTCTTTTTCAGGTTCACTCTGTCATGAGCTGTGATGGCTATTACTTGTGTATTAGAGTCAAATCTACTATTCTGTCTATTCTTTTGATTCAGAGGGTTATGCTAAATTTGACTTCATGCATCTCTTGGTTTTGGCTTCTAAAGTCTACAGTTAGTTTTAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

445

Amino Acids

50.59

Weight (kDa)

9.83

Isoelectric Point (pI)

43.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 93 - 214 1.9e-49 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1319
AccII CGCG 1 cut(s) 330
AciI CCGC 3 cut(s) 582, 643, 953
AcsI RAATTY 2 cut(s) 488, 1279
AcvI CACGTG 1 cut(s) 887
AfaI GTAC 5 cut(s) 163, 385, 789, 1039, 1049
AfiI CCNNNNNNNGG 1 cut(s) 691
AflIII ACRYGT 2 cut(s) 886, 913
AgsI TTSAA 3 cut(s) 415, 481, 764
AhlI ACTAGT 1 cut(s) 164
AjnI CCWGG 1 cut(s) 576
AluBI AGCT 5 cut(s) 146, 151, 418, 604, 1203
AluI AGCT 5 cut(s) 146, 151, 418, 604, 1203
Alw21I GWGCWC 1 cut(s) 628
Alw26I GTCTC 2 cut(s) 561, 762
AoxI GGCC 3 cut(s) 37, 752, 954
ApeKI GCWGC 3 cut(s) 143, 674, 950
ApoI RAATTY 2 cut(s) 488, 1279
Asp700I GAANNNNTTC 2 cut(s) 231, 735
AspLEI GCGC 1 cut(s) 440
AspS9I GGNCC 1 cut(s) 955
AsuHPI GGTGA 2 cut(s) 674, 1148
BbrPI CACGTG 1 cut(s) 887
BbsI GAAGAC 2 cut(s) 374, 1066
Bbv12I GWGCWC 1 cut(s) 628
BbvI GCAGC 3 cut(s) 155, 661, 962
BccI CCATC 5 cut(s) 50, 443, 687, 721, 1202
BceAI ACGGC 1 cut(s) 739
BciT130I CCWGG 1 cut(s) 578
BcoDI GTCTC 2 cut(s) 561, 762
BcuI ACTAGT 1 cut(s) 164
BfaI CTAG 5 cut(s) 45, 165, 222, 783, 947
BfmI CTRYAG 2 cut(s) 141, 1320
BglII AGATCT 1 cut(s) 1119
BisI GCNGC 4 cut(s) 144, 675, 951, 954
BlpI GCTNAGC 1 cut(s) 147
BlsI GCNGC 4 cut(s) 145, 676, 952, 955
BmcAI AGTACT 1 cut(s) 163
Bme1390I CCNGG 1 cut(s) 578
BmgT120I GGNCC 1 cut(s) 955
BmiI GGNNCC 1 cut(s) 468
BmrFI CCNGG 1 cut(s) 578
BmsI GCATC 4 cut(s) 665, 934, 991, 1302
BpiI GAAGAC 2 cut(s) 374, 1066
Bpu1102I GCTNAGC 1 cut(s) 147
BsaAI YACGTR 2 cut(s) 887, 970
BsaJI CCNNGG 2 cut(s) 519, 577
Bsc4I CCNNNNNNNGG 1 cut(s) 691
Bse1I ACTGG 1 cut(s) 201
Bse3DI GCAATG 1 cut(s) 803
BseBI CCWGG 1 cut(s) 578
BseDI CCNNGG 2 cut(s) 519, 577
BseGI GGATG 2 cut(s) 454, 739
BseLI CCNNNNNNNGG 1 cut(s) 691
BseMI GCAATG 1 cut(s) 803
BseMII CTCAG 2 cut(s) 138, 149
BseNI ACTGG 1 cut(s) 201
BseXI GCAGC 3 cut(s) 155, 661, 962
BsgI GTGCAG 1 cut(s) 480
Bsh1236I CGCG 1 cut(s) 330
BshFI GGCC 3 cut(s) 39, 754, 956
BsiHKAI GWGCWC 1 cut(s) 628
BslI CCNNNNNNNGG 1 cut(s) 691
BsmAI GTCTC 2 cut(s) 561, 762
BsmBI CGTCTC 1 cut(s) 762
BsnI GGCC 3 cut(s) 39, 754, 956
Bsp1286I GDGCHC 1 cut(s) 628
Bsp1407I TGTACA 1 cut(s) 1037
Bsp143I GATC 3 cut(s) 49, 236, 1119
Bsp1720I GCTNAGC 1 cut(s) 147
BspACI CCGC 3 cut(s) 582, 643, 953
BspANI GGCC 3 cut(s) 39, 754, 956
BspCNI CTCAG 2 cut(s) 139, 148
BspFNI CGCG 1 cut(s) 330
BspHI TCATGA 2 cut(s) 52, 1197
BspLI GGNNCC 1 cut(s) 468
BspMAI CTGCAG 1 cut(s) 145
BsrDI GCAATG 1 cut(s) 803
BsrGI TGTACA 1 cut(s) 1037
BsrI ACTGG 1 cut(s) 201
BssECI CCNNGG 2 cut(s) 519, 577
BssMI GATC 3 cut(s) 49, 236, 1119
BssT1I CCWWGG 1 cut(s) 519
Bst2UI CCWGG 1 cut(s) 578
Bst4CI ACNGT 1 cut(s) 1324
Bst6I CTCTTC 1 cut(s) 765
BstAUI TGTACA 1 cut(s) 1037
BstBAI YACGTR 2 cut(s) 887, 970
BstC8I GCNNGC 2 cut(s) 20, 624
BstDEI CTNAG 5 cut(s) 26, 108, 135, 147, 152
BstF5I GGATG 2 cut(s) 454, 739
BstFNI CGCG 1 cut(s) 330
BstHHI GCGC 1 cut(s) 440
BstKTI GATC 3 cut(s) 52, 239, 1122
BstMAI GTCTC 2 cut(s) 561, 762
BstMBI GATC 3 cut(s) 49, 236, 1119
BstMWI GCNNNNNNNGC 5 cut(s) 36, 176, 435, 970, 1209
BstNI CCWGG 1 cut(s) 578
BstNSI RCATGY 2 cut(s) 797, 917
BstSCI CCNGG 1 cut(s) 576
BstSFI CTRYAG 2 cut(s) 141, 1320
BstUI CGCG 1 cut(s) 330
BstV1I GCAGC 3 cut(s) 155, 661, 962
BstV2I GAAGAC 2 cut(s) 374, 1066
BstX2I RGATCY 1 cut(s) 1119
BstXI CCANNNNNNTGG 1 cut(s) 584
BstYI RGATCY 1 cut(s) 1119
BsuRI GGCC 3 cut(s) 39, 754, 956
BtsCI GGATG 2 cut(s) 454, 739
BtsI GCAGTG 1 cut(s) 651
BtsIMutI CAGTG 1 cut(s) 651
Cac8I GCNNGC 2 cut(s) 20, 624
CciI TCATGA 2 cut(s) 52, 1197
CfoI GCGC 1 cut(s) 440
Cfr13I GGNCC 1 cut(s) 955
CseI GACGC 1 cut(s) 11
Csp6I GTAC 5 cut(s) 162, 384, 788, 1038, 1048
CspCI CAANNNNNGTGG 2 cut(s) 241, 276
CviAII CATG 9 cut(s) 53, 206, 794, 800, 914, 959, 998, 1198, 1290
CviQI GTAC 5 cut(s) 162, 384, 788, 1038, 1048
DdeI CTNAG 5 cut(s) 26, 108, 135, 147, 152
DpnI GATC 3 cut(s) 51, 238, 1121
DpnII GATC 3 cut(s) 49, 236, 1119
DraI TTTAAA 3 cut(s) 553, 595, 856
Eam1104I CTCTTC 1 cut(s) 765
EarI CTCTTC 1 cut(s) 765
EciI GGCGGA 1 cut(s) 632
Eco130I CCWWGG 1 cut(s) 519
Eco147I AGGCCT 1 cut(s) 39
Eco72I CACGTG 1 cut(s) 887
EcoRII CCWGG 1 cut(s) 576
EcoT14I CCWWGG 1 cut(s) 519
EcoT22I ATGCAT 3 cut(s) 805, 1006, 1295
ErhI CCWWGG 1 cut(s) 519
Esp3I CGTCTC 1 cut(s) 762
FaeI CATG 9 cut(s) 56, 209, 797, 803, 917, 962, 1001, 1201, 1293
FatI CATG 9 cut(s) 52, 205, 793, 799, 913, 958, 997, 1197, 1289
FblI GTMKAC 1 cut(s) 1319
Fnu4HI GCNGC 4 cut(s) 144, 675, 951, 954
FokI GGATG 2 cut(s) 461, 726
Fsp4HI GCNGC 4 cut(s) 144, 675, 951, 954
FspBI CTAG 5 cut(s) 45, 165, 222, 783, 947
GlaI GCGC 1 cut(s) 439
GluI GCNGC 4 cut(s) 144, 675, 951, 954
HaeIII GGCC 3 cut(s) 39, 754, 956
HgaI GACGC 1 cut(s) 11
HhaI GCGC 1 cut(s) 440
Hin1II CATG 9 cut(s) 56, 209, 797, 803, 917, 962, 1001, 1201, 1293
Hin6I GCGC 1 cut(s) 438
HinP1I GCGC 1 cut(s) 438
HinfI GANTC 4 cut(s) 122, 935, 1230, 1261
HphI GGTGA 2 cut(s) 674, 1148
Hpy166II GTNNAC 4 cut(s) 386, 530, 1189, 1320
Hpy188I TCNGA 3 cut(s) 138, 448, 1266
Hpy188III TCNNGA 4 cut(s) 53, 539, 764, 1198
Hpy8I GTNNAC 4 cut(s) 386, 530, 1189, 1320
HpyAV CCTTC 3 cut(s) 725, 1005, 1169
HpyCH4III ACNGT 1 cut(s) 1324
HpyCH4IV ACGT 3 cut(s) 850, 886, 969
HpyCH4V TGCA 7 cut(s) 143, 497, 656, 803, 1004, 1145, 1293
HpyF10VI GCNNNNNNNGC 5 cut(s) 36, 176, 435, 970, 1209
HpyF3I CTNAG 5 cut(s) 26, 108, 135, 147, 152
HpySE526I ACGT 3 cut(s) 850, 886, 969
Hsp92II CATG 9 cut(s) 56, 209, 797, 803, 917, 962, 1001, 1201, 1293
HspAI GCGC 1 cut(s) 438
Kzo9I GATC 3 cut(s) 49, 236, 1119
LmnI GCTCC 3 cut(s) 423, 452, 466
Lsp1109I GCAGC 3 cut(s) 155, 661, 962
LweI GCATC 4 cut(s) 665, 934, 991, 1302
MaeI CTAG 5 cut(s) 45, 165, 222, 783, 947
MaeII ACGT 3 cut(s) 850, 886, 969
MalI GATC 3 cut(s) 51, 238, 1121
MboI GATC 3 cut(s) 49, 236, 1119
MboII GAAGA 9 cut(s) 112, 117, 124, 189, 379, 740, 752, 1066, 1093
MfeI CAATTG 1 cut(s) 471
MflI RGATCY 1 cut(s) 1119
MhlI GDGCHC 1 cut(s) 628
MluCI AATT 6 cut(s) 172, 471, 488, 571, 1041, 1279
MlyI GAGTC 1 cut(s) 1239
MmeI TCCRAC 1 cut(s) 767
Mph1103I ATGCAT 3 cut(s) 805, 1006, 1295
MroXI GAANNNNTTC 2 cut(s) 231, 735
MseI TTAA 7 cut(s) 510, 552, 594, 816, 855, 1044, 1128
MslI CAYNNNNRTG 2 cut(s) 687, 798
MspA1I CMGCKG 2 cut(s) 146, 953
MspR9I CCNGG 1 cut(s) 578
MssI GTTTAAAC 1 cut(s) 595
MunI CAATTG 1 cut(s) 471
MvaI CCWGG 1 cut(s) 578
MvnI CGCG 1 cut(s) 330
MwoI GCNNNNNNNGC 5 cut(s) 36, 176, 435, 970, 1209
NdeII GATC 3 cut(s) 49, 236, 1119
NlaIII CATG 9 cut(s) 56, 209, 797, 803, 917, 962, 1001, 1201, 1293
NlaIV GGNNCC 1 cut(s) 468
NsiI ATGCAT 3 cut(s) 805, 1006, 1295
NspI RCATGY 2 cut(s) 797, 917
PagI TCATGA 2 cut(s) 52, 1197
PceI AGGCCT 1 cut(s) 39
PciI ACATGT 1 cut(s) 913
PcsI WCGNNNNNNNCGW 1 cut(s) 19
PdmI GAANNNNTTC 2 cut(s) 231, 735
PfeI GAWTC 3 cut(s) 122, 935, 1261
PkrI GCNGC 4 cut(s) 145, 676, 952, 955
PleI GAGTC 1 cut(s) 1238
PmaCI CACGTG 1 cut(s) 887
PmeI GTTTAAAC 1 cut(s) 595
PmlI CACGTG 1 cut(s) 887
PpsI GAGTC 1 cut(s) 1238
Ppu21I YACGTR 2 cut(s) 887, 970
PscI ACATGT 1 cut(s) 913
Psp6I CCWGG 1 cut(s) 576
PspCI CACGTG 1 cut(s) 887
PspGI CCWGG 1 cut(s) 576
PspN4I GGNNCC 1 cut(s) 468
PspPI GGNCC 1 cut(s) 955
PstI CTGCAG 1 cut(s) 145
PsuI RGATCY 1 cut(s) 1119
PvuII CAGCTG 1 cut(s) 146
RsaI GTAC 5 cut(s) 163, 385, 789, 1039, 1049
RsaNI GTAC 5 cut(s) 162, 384, 788, 1038, 1048
RseI CAYNNNNRTG 2 cut(s) 687, 798
SaqAI TTAA 7 cut(s) 510, 552, 594, 816, 855, 1044, 1128
SatI GCNGC 4 cut(s) 144, 675, 951, 954
Sau3AI GATC 3 cut(s) 49, 236, 1119
Sau96I GGNCC 1 cut(s) 955
ScaI AGTACT 1 cut(s) 163
SchI GAGTC 1 cut(s) 1239
ScrFI CCNGG 1 cut(s) 578
SduI GDGCHC 1 cut(s) 628
SfaNI GCATC 4 cut(s) 665, 934, 991, 1302
SfcI CTRYAG 2 cut(s) 141, 1320
SmiMI CAYNNNNRTG 2 cut(s) 687, 798
SpeI ACTAGT 1 cut(s) 164
Sse9I AATT 6 cut(s) 172, 471, 488, 571, 1041, 1279
SseBI AGGCCT 1 cut(s) 39
SsiI CCGC 3 cut(s) 582, 643, 953
SspMI CTAG 5 cut(s) 45, 165, 222, 783, 947
StuI AGGCCT 1 cut(s) 39
StyD4I CCNGG 1 cut(s) 576
StyI CCWWGG 1 cut(s) 519
TaaI ACNGT 1 cut(s) 1324
TaiI ACGT 3 cut(s) 853, 889, 972
TaqI TCGA 2 cut(s) 235, 910
TasI AATT 6 cut(s) 172, 471, 488, 571, 1041, 1279
TatI WGTACW 2 cut(s) 161, 1037
TauI GCSGC 1 cut(s) 956
TfiI GAWTC 3 cut(s) 122, 935, 1261
Tru1I TTAA 7 cut(s) 510, 552, 594, 816, 855, 1044, 1128
Tru9I TTAA 7 cut(s) 510, 552, 594, 816, 855, 1044, 1128
TscAI CASTG 1 cut(s) 658
TseI GCWGC 3 cut(s) 143, 674, 950
TspDTI ATGAA 4 cut(s) 728, 879, 927, 1278
TspRI CASTG 1 cut(s) 658
XapI RAATTY 2 cut(s) 488, 1279
XceI RCATGY 2 cut(s) 797, 917
XmiI GTMKAC 1 cut(s) 1319
XmnI GAANNNNTTC 2 cut(s) 231, 735
XspI CTAG 5 cut(s) 45, 165, 222, 783, 947
ZrmI AGTACT 1 cut(s) 163
Zsp2I ATGCAT 3 cut(s) 805, 1006, 1295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.