Rroxscaffold_5G00370570

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000847
Physical Location & Seq
Reverse (-)
52259514 .. 52263842
4329 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00370570.1

Sequence Viewer

Length: 1383 bp
ATGGGAAAAAGACGAGGCAAGCGTCTAAGCAAAAGAGGCCTAACTAGAGATCATGATGGTAGGATTAGGAAGATAGACAAGAGTTTTACAAAGGAAAAGAAGAAATCTAAGAAGATAACCGAATCTTCCTTGCCTCAGACTGCAGCTGAGCTTAGTAGTAGTGCCAATTCTGCTTCCAATAAAGCAACTTCTTCTAGTCATGCTAATCAAGCTACTAGAGAAAGGCTTCGATCAAAAAACAAAGCAAAGGATAGTGGGCAATCACATTCTGGTTTTATCTTTATGTGTAATGGAAAGACTAAACCCGAATGTTATCGGTATCGCGTTTTTGGGCTACCAATGGCAAAAGTAGAAGTTGTGAAGACAATCAAACAGGGTACACATCTATTTTTATATGACTTTGACTTGAAGCTCCTTTATGGCACATACAGTGCAACTTCGGATGGAGCATTAGATTTGGAGCCAATTGCGTTCAAGGGAAAATTTCCTGCACAGGTCAGGTTTAAGATTTCCAAGGATTGTTTACCACTTCACGAAAGTTCTTTTAAAACAGCCATAAGAGACAATTACCAGGGCGGTGGTAAGTTTAAACAAGAGCTGAATAACAAACAGGTGCGTGCTCTAATCTCATTGTTCCGCCCAATCACTGCATCAATGCCTACTTCTGCTGCCCATCACCGAGATGTGGCAAGAACAGGCTCATATCCATCTCCTATTAGGGAAGAAGGATTTCATCCAACAGCAAGGCCGTCTCTTCAAGAGGGCTTATATTTTTCTAGTGTACAACGTGCTCATGCATTGCCACTTCTTAATACGCAATCCATACAGGTTGTTCCATATCCACGTTTTAAAAAATATGAAGCAGAAAGATGTGTCGCATGTGTTCAGCCTCCCATAGAGCCTCGACATGTTGTTCCGCATCAGCCAGATTCGTATTATCTAGCAGCGGCCCATGAGCCATACGTGCCTGAAAAACCTTTCTTGTCCTCTCATGATGCTTATAGAAGGCATGGGGTCACACTTCCACAGGAAATGGTTCCTAGGGATCAAGATGTTTCATATGGAAGTGTGTATCGCTTGTCACAGTTGCTGAAGGAGAGAGAAAGAGATATTGTTTCCCATTCCGATTATATTGCTGAAACCTATAGTCGACACTTGCCGACTCATCCAGCCTCACAGTTTAGGTTGCAGTCACATTCTTCGGCATCATCTTATGAGTTACCATCAACTTACCAACCATCTTATCAAGCAACGACCCATCAGGATCAGAACCCGGTGGATCATGATCCTCTTCAGAGACCATTGCATGGAAGCTCTAATGAGGCAAGTTTGCCAATCTGTTCTCACTTGTCTTCCACTGGAGCTGCCCCTACTACTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

460

Amino Acids

51.69

Weight (kDa)

9.63

Isoelectric Point (pI)

46.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 91 - 212 2e-49 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1307
AccI GTMKAC 1 cut(s) 1150
AccII CGCG 1 cut(s) 324
AciI CCGC 4 cut(s) 576, 637, 917, 947
AclWI GGATC 4 cut(s) 1053, 1272, 1280, 1287
AcsI RAATTY 1 cut(s) 482
AcuI CTGAAG 2 cut(s) 1112, 1277
AfaI GTAC 2 cut(s) 379, 783
AfiI CCNNNNNNNGG 2 cut(s) 685, 1307
AflIII ACRYGT 1 cut(s) 907
AgsI TTSAA 3 cut(s) 409, 475, 758
AjnI CCWGG 1 cut(s) 570
AluBI AGCT 7 cut(s) 146, 151, 212, 412, 598, 1314, 1364
AluI AGCT 7 cut(s) 146, 151, 212, 412, 598, 1314, 1364
Alw21I GWGCWC 2 cut(s) 622, 793
Alw26I GTCTC 3 cut(s) 555, 756, 1291
AlwI GGATC 4 cut(s) 1053, 1272, 1280, 1287
AlwNI CAGNNNCTG 1 cut(s) 1090
AoxI GGCC 3 cut(s) 37, 746, 948
ApeKI GCWGC 4 cut(s) 143, 668, 944, 1364
ApoI RAATTY 1 cut(s) 482
Asp700I GAANNNNTTC 3 cut(s) 225, 729, 1035
AspA2I CCTAGG 1 cut(s) 1040
AspS9I GGNCC 1 cut(s) 949
AsuC2I CCSGG 1 cut(s) 1274
AsuHPI GGTGA 1 cut(s) 668
AvrII CCTAGG 1 cut(s) 1040
BbsI GAAGAC 2 cut(s) 368, 1344
Bbv12I GWGCWC 2 cut(s) 622, 793
BbvI GCAGC 4 cut(s) 155, 655, 956, 1351
BccI CCATC 7 cut(s) 50, 437, 681, 715, 1231, 1246, 1266
BceAI ACGGC 1 cut(s) 733
BcgI CGANNNNNNTGC 2 cut(s) 1115, 1149
BciT130I CCWGG 1 cut(s) 572
BcnI CCSGG 1 cut(s) 1274
BcoDI GTCTC 3 cut(s) 555, 756, 1291
BfaI CTAG 6 cut(s) 45, 195, 216, 777, 941, 1041
BfmI CTRYAG 2 cut(s) 141, 1144
BisI GCNGC 5 cut(s) 144, 669, 945, 948, 1365
BlnI CCTAGG 1 cut(s) 1040
BlpI GCTNAGC 1 cut(s) 147
BlsI GCNGC 5 cut(s) 145, 670, 946, 949, 1366
Bme1390I CCNGG 2 cut(s) 572, 1274
BmgT120I GGNCC 1 cut(s) 949
BmiI GGNNCC 2 cut(s) 462, 1038
BmrFI CCNGG 2 cut(s) 572, 1274
BmsI GCATC 4 cut(s) 659, 928, 985, 1214
BpiI GAAGAC 2 cut(s) 368, 1344
BpmI CTGGAG 1 cut(s) 1380
Bpu1102I GCTNAGC 1 cut(s) 147
BpuMI CCSGG 1 cut(s) 1274
BsaAI YACGTR 1 cut(s) 964
BsaBI GATNNNNATC 1 cut(s) 1284
BsaI GGTCTC 1 cut(s) 1291
BsaJI CCNNGG 3 cut(s) 513, 571, 1040
Bsc4I CCNNNNNNNGG 2 cut(s) 685, 1307
Bse1I ACTGG 1 cut(s) 1363
Bse3DI GCAATG 2 cut(s) 797, 1301
Bse8I GATNNNNATC 1 cut(s) 1284
BseBI CCWGG 1 cut(s) 572
BseDI CCNNGG 3 cut(s) 513, 571, 1040
BseGI GGATG 3 cut(s) 448, 733, 1165
BseJI GATNNNNATC 1 cut(s) 1284
BseLI CCNNNNNNNGG 2 cut(s) 685, 1307
BseMI GCAATG 2 cut(s) 797, 1301
BseMII CTCAG 2 cut(s) 138, 149
BseNI ACTGG 1 cut(s) 1363
BseXI GCAGC 4 cut(s) 155, 655, 956, 1351
BsgI GTGCAG 1 cut(s) 474
Bsh1236I CGCG 1 cut(s) 324
BshFI GGCC 3 cut(s) 39, 748, 950
BsiHKAI GWGCWC 2 cut(s) 622, 793
BsiSI CCGG 1 cut(s) 1274
BslI CCNNNNNNNGG 2 cut(s) 685, 1307
BsmAI GTCTC 3 cut(s) 555, 756, 1291
BsmBI CGTCTC 1 cut(s) 756
BsnI GGCC 3 cut(s) 39, 748, 950
Bso31I GGTCTC 1 cut(s) 1291
Bsp1286I GDGCHC 2 cut(s) 622, 793
Bsp1407I TGTACA 1 cut(s) 781
Bsp143I GATC 6 cut(s) 49, 230, 1045, 1264, 1279, 1285
Bsp1720I GCTNAGC 1 cut(s) 147
BspACI CCGC 4 cut(s) 576, 637, 917, 947
BspANI GGCC 3 cut(s) 39, 748, 950
BspCNI CTCAG 2 cut(s) 139, 148
BspFNI CGCG 1 cut(s) 324
BspHI TCATGA 3 cut(s) 52, 991, 1282
BspLI GGNNCC 2 cut(s) 462, 1038
BspMAI CTGCAG 1 cut(s) 145
BspPI GGATC 4 cut(s) 1053, 1272, 1280, 1287
BspTNI GGTCTC 1 cut(s) 1291
BsrDI GCAATG 2 cut(s) 797, 1301
BsrGI TGTACA 1 cut(s) 781
BsrI ACTGG 1 cut(s) 1363
BssECI CCNNGG 3 cut(s) 513, 571, 1040
BssMI GATC 6 cut(s) 49, 230, 1045, 1264, 1279, 1285
BssT1I CCWWGG 2 cut(s) 513, 1040
Bst2UI CCWGG 1 cut(s) 572
Bst4CI ACNGT 3 cut(s) 431, 1086, 1179
Bst6I CTCTTC 2 cut(s) 759, 1296
BstAUI TGTACA 1 cut(s) 781
BstBAI YACGTR 1 cut(s) 964
BstC8I GCNNGC 2 cut(s) 20, 618
BstDEI CTNAG 5 cut(s) 26, 108, 135, 147, 152
BstF5I GGATG 3 cut(s) 448, 733, 1165
BstFNI CGCG 1 cut(s) 324
BstKTI GATC 6 cut(s) 52, 233, 1048, 1267, 1282, 1288
BstMAI GTCTC 3 cut(s) 555, 756, 1291
BstMBI GATC 6 cut(s) 49, 230, 1045, 1264, 1279, 1285
BstMWI GCNNNNNNNGC 4 cut(s) 36, 170, 209, 964
BstNI CCWGG 1 cut(s) 572
BstNSI RCATGY 2 cut(s) 882, 911
BstSCI CCNGG 2 cut(s) 570, 1272
BstSFI CTRYAG 2 cut(s) 141, 1144
BstUI CGCG 1 cut(s) 324
BstV1I GCAGC 4 cut(s) 155, 655, 956, 1351
BstV2I GAAGAC 2 cut(s) 368, 1344
BstXI CCANNNNNNTGG 1 cut(s) 578
BsuRI GGCC 3 cut(s) 39, 748, 950
BtsCI GGATG 3 cut(s) 448, 733, 1165
BtsI GCAGTG 1 cut(s) 645
BtsIMutI CAGTG 3 cut(s) 436, 645, 1356
Cac8I GCNNGC 2 cut(s) 20, 618
CaiI CAGNNNCTG 1 cut(s) 1090
CciI TCATGA 3 cut(s) 52, 991, 1282
Cfr13I GGNCC 1 cut(s) 949
CseI GACGC 1 cut(s) 11
Csp6I GTAC 2 cut(s) 378, 782
CviQI GTAC 2 cut(s) 378, 782
DdeI CTNAG 5 cut(s) 26, 108, 135, 147, 152
DpnI GATC 6 cut(s) 51, 232, 1047, 1266, 1281, 1287
DpnII GATC 6 cut(s) 49, 230, 1045, 1264, 1279, 1285
DraI TTTAAA 3 cut(s) 547, 589, 850
Eam1104I CTCTTC 2 cut(s) 759, 1296
EarI CTCTTC 2 cut(s) 759, 1296
EciI GGCGGA 1 cut(s) 626
Eco130I CCWWGG 2 cut(s) 513, 1040
Eco147I AGGCCT 1 cut(s) 39
Eco31I GGTCTC 1 cut(s) 1291
Eco57I CTGAAG 2 cut(s) 1112, 1277
EcoRII CCWGG 1 cut(s) 570
EcoT14I CCWWGG 2 cut(s) 513, 1040
EcoT22I ATGCAT 1 cut(s) 799
ErhI CCWWGG 2 cut(s) 513, 1040
Esp3I CGTCTC 1 cut(s) 756
FauNDI CATATG 1 cut(s) 1060
FblI GTMKAC 1 cut(s) 1150
Fnu4HI GCNGC 5 cut(s) 144, 669, 945, 948, 1365
FokI GGATG 3 cut(s) 455, 720, 1152
Fsp4HI GCNGC 5 cut(s) 144, 669, 945, 948, 1365
FspBI CTAG 6 cut(s) 45, 195, 216, 777, 941, 1041
GluI GCNGC 5 cut(s) 144, 669, 945, 948, 1365
GsuI CTGGAG 1 cut(s) 1380
HaeIII GGCC 3 cut(s) 39, 748, 950
HapII CCGG 1 cut(s) 1274
HgaI GACGC 1 cut(s) 11
HincII GTYRAC 1 cut(s) 1151
HindII GTYRAC 1 cut(s) 1151
HinfI GANTC 3 cut(s) 122, 929, 1162
HpaII CCGG 1 cut(s) 1274
HphI GGTGA 1 cut(s) 668
Hpy166II GTNNAC 4 cut(s) 380, 524, 782, 1151
Hpy188I TCNGA 5 cut(s) 138, 442, 1126, 1269, 1296
Hpy188III TCNNGA 7 cut(s) 53, 533, 758, 992, 1049, 1262, 1283
Hpy8I GTNNAC 4 cut(s) 380, 524, 782, 1151
HpyAV CCTTC 3 cut(s) 719, 999, 1087
HpyCH4III ACNGT 3 cut(s) 431, 1086, 1179
HpyCH4IV ACGT 3 cut(s) 787, 844, 963
HpyCH4V TGCA 7 cut(s) 143, 434, 491, 650, 797, 1189, 1306
HpyF10VI GCNNNNNNNGC 4 cut(s) 36, 170, 209, 964
HpyF3I CTNAG 5 cut(s) 26, 108, 135, 147, 152
HpySE526I ACGT 3 cut(s) 787, 844, 963
Kzo9I GATC 6 cut(s) 49, 230, 1045, 1264, 1279, 1285
LmnI GCTCC 4 cut(s) 417, 446, 460, 1361
Lsp1109I GCAGC 4 cut(s) 155, 655, 956, 1351
LweI GCATC 4 cut(s) 659, 928, 985, 1214
MaeI CTAG 6 cut(s) 45, 195, 216, 777, 941, 1041
MaeII ACGT 3 cut(s) 787, 844, 963
MaeIII GTNAC 4 cut(s) 1015, 1080, 1191, 1218
MalI GATC 6 cut(s) 51, 232, 1047, 1266, 1281, 1287
MboI GATC 6 cut(s) 49, 230, 1045, 1264, 1279, 1285
MfeI CAATTG 1 cut(s) 465
MhlI GDGCHC 2 cut(s) 622, 793
MluCI AATT 4 cut(s) 166, 465, 482, 565
MlyI GAGTC 1 cut(s) 1156
MmeI TCCRAC 1 cut(s) 761
Mph1103I ATGCAT 1 cut(s) 799
MroXI GAANNNNTTC 3 cut(s) 225, 729, 1035
MseI TTAA 5 cut(s) 504, 546, 588, 810, 849
MslI CAYNNNNRTG 1 cut(s) 681
MspA1I CMGCKG 2 cut(s) 146, 947
MspI CCGG 1 cut(s) 1274
MspR9I CCNGG 2 cut(s) 572, 1274
MssI GTTTAAAC 1 cut(s) 589
MunI CAATTG 1 cut(s) 465
MvaI CCWGG 1 cut(s) 572
MvnI CGCG 1 cut(s) 324
MwoI GCNNNNNNNGC 4 cut(s) 36, 170, 209, 964
NciI CCSGG 1 cut(s) 1274
NdeI CATATG 1 cut(s) 1060
NdeII GATC 6 cut(s) 49, 230, 1045, 1264, 1279, 1285
NlaIV GGNNCC 2 cut(s) 462, 1038
NmuCI GTSAC 3 cut(s) 1015, 1080, 1191
NsiI ATGCAT 1 cut(s) 799
NspI RCATGY 2 cut(s) 882, 911
PagI TCATGA 3 cut(s) 52, 991, 1282
PceI AGGCCT 1 cut(s) 39
PciI ACATGT 1 cut(s) 907
PcsI WCGNNNNNNNCGW 1 cut(s) 19
PdmI GAANNNNTTC 3 cut(s) 225, 729, 1035
PfeI GAWTC 2 cut(s) 122, 929
PflMI CCANNNNNTGG 1 cut(s) 1307
PkrI GCNGC 5 cut(s) 145, 670, 946, 949, 1366
PleI GAGTC 1 cut(s) 1156
PmeI GTTTAAAC 1 cut(s) 589
PpsI GAGTC 1 cut(s) 1156
Ppu21I YACGTR 1 cut(s) 964
PscI ACATGT 1 cut(s) 907
Psp6I CCWGG 1 cut(s) 570
PspGI CCWGG 1 cut(s) 570
PspN4I GGNNCC 2 cut(s) 462, 1038
PspPI GGNCC 1 cut(s) 949
PstI CTGCAG 1 cut(s) 145
PstNI CAGNNNCTG 1 cut(s) 1090
PvuII CAGCTG 1 cut(s) 146
RsaI GTAC 2 cut(s) 379, 783
RsaNI GTAC 2 cut(s) 378, 782
RseI CAYNNNNRTG 1 cut(s) 681
SalI GTCGAC 1 cut(s) 1149
SaqAI TTAA 5 cut(s) 504, 546, 588, 810, 849
SatI GCNGC 5 cut(s) 144, 669, 945, 948, 1365
Sau3AI GATC 6 cut(s) 49, 230, 1045, 1264, 1279, 1285
Sau96I GGNCC 1 cut(s) 949
SchI GAGTC 1 cut(s) 1156
ScrFI CCNGG 2 cut(s) 572, 1274
SduI GDGCHC 2 cut(s) 622, 793
SfaNI GCATC 4 cut(s) 659, 928, 985, 1214
SfcI CTRYAG 2 cut(s) 141, 1144
SmiMI CAYNNNNRTG 1 cut(s) 681
Sse9I AATT 4 cut(s) 166, 465, 482, 565
SseBI AGGCCT 1 cut(s) 39
SsiI CCGC 4 cut(s) 576, 637, 917, 947
SspMI CTAG 6 cut(s) 45, 195, 216, 777, 941, 1041
StuI AGGCCT 1 cut(s) 39
StyD4I CCNGG 2 cut(s) 570, 1272
StyI CCWWGG 2 cut(s) 513, 1040
TaaI ACNGT 3 cut(s) 431, 1086, 1179
TaiI ACGT 3 cut(s) 790, 847, 966
TaqI TCGA 3 cut(s) 229, 904, 1150
TasI AATT 4 cut(s) 166, 465, 482, 565
TatI WGTACW 1 cut(s) 781
TauI GCSGC 1 cut(s) 950
TfiI GAWTC 2 cut(s) 122, 929
Tru1I TTAA 5 cut(s) 504, 546, 588, 810, 849
Tru9I TTAA 5 cut(s) 504, 546, 588, 810, 849
TscAI CASTG 3 cut(s) 436, 652, 1363
TseFI GTSAC 3 cut(s) 1015, 1080, 1191
TseI GCWGC 4 cut(s) 143, 668, 944, 1364
Tsp45I GTSAC 3 cut(s) 1015, 1080, 1191
TspDTI ATGAA 3 cut(s) 722, 873, 1047
TspRI CASTG 3 cut(s) 436, 652, 1363
Van91I CCANNNNNTGG 1 cut(s) 1307
XapI RAATTY 1 cut(s) 482
XceI RCATGY 2 cut(s) 882, 911
XmaJI CCTAGG 1 cut(s) 1040
XmiI GTMKAC 1 cut(s) 1150
XmnI GAANNNNTTC 3 cut(s) 225, 729, 1035
XspI CTAG 6 cut(s) 45, 195, 216, 777, 941, 1041
Zsp2I ATGCAT 1 cut(s) 799
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.