Rh4CG308600

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000847
Physical Location & Seq
Reverse (-)
57010004 .. 57014239
4236 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG308600.1

Sequence Viewer

Length: 1323 bp
ATGTGCAGTCGTCTTCAACCTCCACCACTATTCCACCTCGATTTCCGGCCTATATCCATGGGAAAAAGACGAGGCAAGCGTCTAAGCAAAAGAGGCCTAACTAGAGATCATGATGGTAGGATTAGGAGGATAGACAAGAGTTTTACAAAGGAAAAGAAGAAATCTAAGAAGATAACCGAATCTTCCTTGCCTCAGACTGCAGCTGAGCTTAGTAGTACTAGTGCCAATTCTGCTTCCAATAAAGCAACTTCTTCTAGTCATGCTAATCAAGCTACTAGAGAAAGGCTTCGATCAAAAAACAAAGGGAAGGATAGTGGGCAATCACATTCTGGTTTTATCTTTATGTGTAATGGAAAGACTAAACCCGAATGTTATCAGTATCGCGTTTTTGGGCTACCAATGGCAAAAGTAGAAGTTGTGAAGACAATCAAACAGGGTACACATCTATTTTTATATGACTTTGACTTGAAGCTCCTTTATGGCACATACAGCGCAACTTCGGATGGAGCATTAGATTTGGAGCCAATTGCATTCAAGGGAAAATTTCCTGCACAGGTGCGTGCTCTAATCTCATTGTTCCGCCCAATCACTGCATCAATGCCTACTTCTGCTGCCCATCACCGAGATGTGGCAAGAACAGGCTCATATCCATCTCCTATTAGTGAAGAAGGATTTCATCCAACAGCAAGGCCGTCTCTTCAAGAGGGCTTATATTTATCTAGCGTACAACATGCTCATGCATTGCCACTTCTTAATACGCAATCCATACAGGTTGTTCCATATCCACGTTTTAAAAAATATGAAGCAGAAAGATGTGTGGCACGTGTTCAGCCTCCCATAGAGCCTAGACATGTTGTTCAGCATCAGCCAGATTCATATTATCTAGCAGCGGCCCATGAGCCATACGTGCCTGAAAAACCTTTCTTGACCTCCCATGATGCATATAGAAGGCATGGGGTCACACTTCCACAGGAGATGGTTCCTAGGGATCAAGATGTTTCATATGGAAGTGTGTATCGCTTGTCACAGTTGCTGAAGGAGAGAGAAAGAGATATTGTTTCCCATTCCGATTATATTGCTGAGACCTATAGTCGACACTTGCCAACTCATCCAGCCTCACAGTTTAGGTTGCAGTCACATTCTTCGGCATCATCTTATGAGTTACCATCAACTTACCAACCATATGATCAAGCAACGACCCATCAGGATCAGAACCCGGTGGATCGTGATCCTCTTCAGAGACCATTGCATGGAAGCTCTAATGAGGCAAGTTTGCCAACCTGTTTTCACTTGTCTTCCACTGGAGCTGCCCGCTACTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

440

Amino Acids

49.64

Weight (kDa)

9.61

Isoelectric Point (pI)

46.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 111 - 197 6.7e-31 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1250
AccI GTMKAC 1 cut(s) 1093
AccII CGCG 1 cut(s) 384
AciI CCGC 3 cut(s) 580, 890, 1312
AclWI GGATC 4 cut(s) 996, 1215, 1223, 1230
AcsI RAATTY 1 cut(s) 542
AcuI CTGAAG 2 cut(s) 1055, 1220
AcvI CACGTG 1 cut(s) 824
AfaI GTAC 3 cut(s) 217, 439, 726
AfiI CCNNNNNNNGG 2 cut(s) 628, 1250
AflIII ACRYGT 2 cut(s) 823, 850
AgsI TTSAA 4 cut(s) 17, 469, 535, 701
AhdI GACNNNNNGTC 1 cut(s) 1089
AhlI ACTAGT 1 cut(s) 218
AluBI AGCT 6 cut(s) 203, 208, 272, 472, 1257, 1307
AluI AGCT 6 cut(s) 203, 208, 272, 472, 1257, 1307
Alw21I GWGCWC 1 cut(s) 565
Alw26I GTCTC 3 cut(s) 699, 1076, 1234
AlwI GGATC 4 cut(s) 996, 1215, 1223, 1230
AlwNI CAGNNNCTG 1 cut(s) 1033
AoxI GGCC 4 cut(s) 47, 94, 689, 891
ApeKI GCWGC 4 cut(s) 200, 611, 887, 1307
ApoI RAATTY 1 cut(s) 542
Asp700I GAANNNNTTC 2 cut(s) 285, 672
AspA2I CCTAGG 1 cut(s) 983
AspLEI GCGC 1 cut(s) 494
AspS9I GGNCC 1 cut(s) 892
AsuC2I CCSGG 1 cut(s) 1217
AsuHPI GGTGA 1 cut(s) 611
AvrII CCTAGG 1 cut(s) 983
BbrPI CACGTG 1 cut(s) 824
BbsI GAAGAC 3 cut(s) 5, 428, 1287
Bbv12I GWGCWC 1 cut(s) 565
BbvI GCAGC 4 cut(s) 212, 598, 899, 1294
BccI CCATC 7 cut(s) 107, 497, 624, 658, 970, 1174, 1209
BceAI ACGGC 1 cut(s) 676
BcgI CGANNNNNNTGC 2 cut(s) 1058, 1092
BclI TGATCA 1 cut(s) 1186
BcnI CCSGG 1 cut(s) 1217
BcoDI GTCTC 3 cut(s) 699, 1076, 1234
BcuI ACTAGT 1 cut(s) 218
BfaI CTAG 8 cut(s) 102, 219, 255, 276, 720, 846, 884, 984
BfmI CTRYAG 2 cut(s) 198, 1087
BisI GCNGC 5 cut(s) 201, 612, 888, 891, 1308
BlnI CCTAGG 1 cut(s) 983
BlpI GCTNAGC 1 cut(s) 204
BlsI GCNGC 5 cut(s) 202, 613, 889, 892, 1309
BmcAI AGTACT 1 cut(s) 217
Bme1390I CCNGG 1 cut(s) 1217
BmeRI GACNNNNNGTC 1 cut(s) 1089
BmgT120I GGNCC 1 cut(s) 892
BmiI GGNNCC 2 cut(s) 522, 981
BmrFI CCNGG 1 cut(s) 1217
BmsI GCATC 4 cut(s) 602, 871, 928, 1157
BpiI GAAGAC 3 cut(s) 5, 428, 1287
BpmI CTGGAG 1 cut(s) 1323
Bpu1102I GCTNAGC 1 cut(s) 204
BpuMI CCSGG 1 cut(s) 1217
BsaAI YACGTR 2 cut(s) 824, 907
BsaBI GATNNNNATC 1 cut(s) 1227
BsaI GGTCTC 2 cut(s) 1076, 1234
BsaJI CCNNGG 2 cut(s) 57, 983
Bsc4I CCNNNNNNNGG 2 cut(s) 628, 1250
Bse1I ACTGG 1 cut(s) 1306
Bse3DI GCAATG 2 cut(s) 740, 1244
Bse8I GATNNNNATC 1 cut(s) 1227
BseDI CCNNGG 2 cut(s) 57, 983
BseGI GGATG 3 cut(s) 508, 676, 1108
BseJI GATNNNNATC 1 cut(s) 1227
BseLI CCNNNNNNNGG 2 cut(s) 628, 1250
BseMI GCAATG 2 cut(s) 740, 1244
BseMII CTCAG 3 cut(s) 195, 206, 1071
BseNI ACTGG 1 cut(s) 1306
BseXI GCAGC 4 cut(s) 212, 598, 899, 1294
BsgI GTGCAG 2 cut(s) 25, 534
Bsh1236I CGCG 1 cut(s) 384
BshFI GGCC 4 cut(s) 49, 96, 691, 893
BsiHKAI GWGCWC 1 cut(s) 565
BsiSI CCGG 2 cut(s) 46, 1217
BslI CCNNNNNNNGG 2 cut(s) 628, 1250
BsmAI GTCTC 3 cut(s) 699, 1076, 1234
BsmBI CGTCTC 1 cut(s) 699
BsmI GAATGC 1 cut(s) 530
BsnI GGCC 4 cut(s) 49, 96, 691, 893
Bso31I GGTCTC 2 cut(s) 1076, 1234
Bsp1286I GDGCHC 1 cut(s) 565
Bsp143I GATC 7 cut(s) 106, 290, 988, 1186, 1207, 1222, 1228
Bsp1720I GCTNAGC 1 cut(s) 204
Bsp19I CCATGG 1 cut(s) 57
BspACI CCGC 3 cut(s) 580, 890, 1312
BspANI GGCC 4 cut(s) 49, 96, 691, 893
BspCNI CTCAG 3 cut(s) 196, 205, 1072
BspFNI CGCG 1 cut(s) 384
BspHI TCATGA 1 cut(s) 109
BspLI GGNNCC 2 cut(s) 522, 981
BspMAI CTGCAG 1 cut(s) 202
BspPI GGATC 4 cut(s) 996, 1215, 1223, 1230
BspTNI GGTCTC 2 cut(s) 1076, 1234
BsrDI GCAATG 2 cut(s) 740, 1244
BsrI ACTGG 1 cut(s) 1306
BssECI CCNNGG 2 cut(s) 57, 983
BssMI GATC 7 cut(s) 106, 290, 988, 1186, 1207, 1222, 1228
BssT1I CCWWGG 2 cut(s) 57, 983
Bst4CI ACNGT 2 cut(s) 1029, 1122
Bst6I CTCTTC 2 cut(s) 702, 1239
BstBAI YACGTR 2 cut(s) 824, 907
BstC8I GCNNGC 3 cut(s) 77, 561, 1312
BstDEI CTNAG 6 cut(s) 83, 165, 192, 204, 209, 1080
BstDSI CCRYGG 1 cut(s) 57
BstF5I GGATG 3 cut(s) 508, 676, 1108
BstFNI CGCG 1 cut(s) 384
BstHHI GCGC 1 cut(s) 494
BstKTI GATC 7 cut(s) 109, 293, 991, 1189, 1210, 1225, 1231
BstMAI GTCTC 3 cut(s) 699, 1076, 1234
BstMBI GATC 7 cut(s) 106, 290, 988, 1186, 1207, 1222, 1228
BstMWI GCNNNNNNNGC 5 cut(s) 93, 230, 269, 489, 907
BstNSI RCATGY 2 cut(s) 734, 854
BstSCI CCNGG 1 cut(s) 1215
BstSFI CTRYAG 2 cut(s) 198, 1087
BstUI CGCG 1 cut(s) 384
BstV1I GCAGC 4 cut(s) 212, 598, 899, 1294
BstV2I GAAGAC 3 cut(s) 5, 428, 1287
BsuRI GGCC 4 cut(s) 49, 96, 691, 893
BtgI CCRYGG 1 cut(s) 57
BtsCI GGATG 3 cut(s) 508, 676, 1108
BtsI GCAGTG 1 cut(s) 588
BtsIMutI CAGTG 2 cut(s) 588, 1299
Cac8I GCNNGC 3 cut(s) 77, 561, 1312
CaiI CAGNNNCTG 1 cut(s) 1033
CciI TCATGA 1 cut(s) 109
CfoI GCGC 1 cut(s) 494
Cfr13I GGNCC 1 cut(s) 892
CseI GACGC 1 cut(s) 68
Csp6I GTAC 3 cut(s) 216, 438, 725
CviQI GTAC 3 cut(s) 216, 438, 725
DdeI CTNAG 6 cut(s) 83, 165, 192, 204, 209, 1080
DpnI GATC 7 cut(s) 108, 292, 990, 1188, 1209, 1224, 1230
DpnII GATC 7 cut(s) 106, 290, 988, 1186, 1207, 1222, 1228
DraI TTTAAA 1 cut(s) 793
DriI GACNNNNNGTC 1 cut(s) 1089
Eam1104I CTCTTC 2 cut(s) 702, 1239
Eam1105I GACNNNNNGTC 1 cut(s) 1089
EarI CTCTTC 2 cut(s) 702, 1239
EciI GGCGGA 1 cut(s) 569
Eco130I CCWWGG 2 cut(s) 57, 983
Eco147I AGGCCT 1 cut(s) 96
Eco31I GGTCTC 2 cut(s) 1076, 1234
Eco57I CTGAAG 2 cut(s) 1055, 1220
Eco72I CACGTG 1 cut(s) 824
EcoT14I CCWWGG 2 cut(s) 57, 983
EcoT22I ATGCAT 2 cut(s) 742, 943
ErhI CCWWGG 2 cut(s) 57, 983
Esp3I CGTCTC 1 cut(s) 699
FauI CCCGC 1 cut(s) 1319
FauNDI CATATG 2 cut(s) 1003, 1183
FbaI TGATCA 1 cut(s) 1186
FblI GTMKAC 1 cut(s) 1093
Fnu4HI GCNGC 5 cut(s) 201, 612, 888, 891, 1308
FokI GGATG 3 cut(s) 515, 663, 1095
Fsp4HI GCNGC 5 cut(s) 201, 612, 888, 891, 1308
FspBI CTAG 8 cut(s) 102, 219, 255, 276, 720, 846, 884, 984
GlaI GCGC 1 cut(s) 493
GluI GCNGC 5 cut(s) 201, 612, 888, 891, 1308
GsuI CTGGAG 1 cut(s) 1323
HaeIII GGCC 4 cut(s) 49, 96, 691, 893
HapII CCGG 2 cut(s) 46, 1217
HgaI GACGC 1 cut(s) 68
HhaI GCGC 1 cut(s) 494
Hin6I GCGC 1 cut(s) 492
HinP1I GCGC 1 cut(s) 492
HincII GTYRAC 1 cut(s) 1094
HindII GTYRAC 1 cut(s) 1094
HinfI GANTC 2 cut(s) 179, 872
HpaII CCGG 2 cut(s) 46, 1217
HphI GGTGA 1 cut(s) 611
Hpy166II GTNNAC 2 cut(s) 440, 1094
Hpy188I TCNGA 5 cut(s) 195, 502, 1069, 1212, 1239
Hpy188III TCNNGA 6 cut(s) 110, 701, 925, 992, 1205, 1226
Hpy8I GTNNAC 2 cut(s) 440, 1094
HpyAV CCTTC 4 cut(s) 301, 662, 942, 1030
HpyCH4III ACNGT 2 cut(s) 1029, 1122
HpyCH4IV ACGT 3 cut(s) 787, 823, 906
HpyCH4V TGCA 9 cut(s) 6, 200, 530, 551, 593, 740, 941, 1132, 1249
HpyF10VI GCNNNNNNNGC 5 cut(s) 93, 230, 269, 489, 907
HpyF3I CTNAG 6 cut(s) 83, 165, 192, 204, 209, 1080
HpySE526I ACGT 3 cut(s) 787, 823, 906
HspAI GCGC 1 cut(s) 492
Ksp22I TGATCA 1 cut(s) 1186
Kzo9I GATC 7 cut(s) 106, 290, 988, 1186, 1207, 1222, 1228
LmnI GCTCC 4 cut(s) 477, 506, 520, 1304
Lsp1109I GCAGC 4 cut(s) 212, 598, 899, 1294
LweI GCATC 4 cut(s) 602, 871, 928, 1157
MaeI CTAG 8 cut(s) 102, 219, 255, 276, 720, 846, 884, 984
MaeII ACGT 3 cut(s) 787, 823, 906
MaeIII GTNAC 4 cut(s) 958, 1023, 1134, 1161
MalI GATC 7 cut(s) 108, 292, 990, 1188, 1209, 1224, 1230
MboI GATC 7 cut(s) 106, 290, 988, 1186, 1207, 1222, 1228
MfeI CAATTG 1 cut(s) 525
MhlI GDGCHC 1 cut(s) 565
MluCI AATT 3 cut(s) 226, 525, 542
MmeI TCCRAC 1 cut(s) 704
Mph1103I ATGCAT 2 cut(s) 742, 943
MroXI GAANNNNTTC 2 cut(s) 285, 672
MseI TTAA 2 cut(s) 753, 792
MslI CAYNNNNRTG 2 cut(s) 624, 735
MspA1I CMGCKG 2 cut(s) 203, 890
MspI CCGG 2 cut(s) 46, 1217
MspR9I CCNGG 1 cut(s) 1217
MunI CAATTG 1 cut(s) 525
Mva1269I GAATGC 1 cut(s) 530
MvnI CGCG 1 cut(s) 384
MwoI GCNNNNNNNGC 5 cut(s) 93, 230, 269, 489, 907
NciI CCSGG 1 cut(s) 1217
NcoI CCATGG 1 cut(s) 57
NdeI CATATG 2 cut(s) 1003, 1183
NdeII GATC 7 cut(s) 106, 290, 988, 1186, 1207, 1222, 1228
NlaIV GGNNCC 2 cut(s) 522, 981
NmuCI GTSAC 3 cut(s) 958, 1023, 1134
NsiI ATGCAT 2 cut(s) 742, 943
NspI RCATGY 2 cut(s) 734, 854
PagI TCATGA 1 cut(s) 109
PceI AGGCCT 1 cut(s) 96
PciI ACATGT 1 cut(s) 850
PcsI WCGNNNNNNNCGW 1 cut(s) 76
PctI GAATGC 1 cut(s) 530
PdmI GAANNNNTTC 2 cut(s) 285, 672
PfeI GAWTC 2 cut(s) 179, 872
PflMI CCANNNNNTGG 1 cut(s) 1250
PkrI GCNGC 5 cut(s) 202, 613, 889, 892, 1309
PmaCI CACGTG 1 cut(s) 824
PmlI CACGTG 1 cut(s) 824
Ppu21I YACGTR 2 cut(s) 824, 907
PscI ACATGT 1 cut(s) 850
PspCI CACGTG 1 cut(s) 824
PspN4I GGNNCC 2 cut(s) 522, 981
PspPI GGNCC 1 cut(s) 892
PstI CTGCAG 1 cut(s) 202
PstNI CAGNNNCTG 1 cut(s) 1033
PvuII CAGCTG 1 cut(s) 203
RsaI GTAC 3 cut(s) 217, 439, 726
RsaNI GTAC 3 cut(s) 216, 438, 725
RseI CAYNNNNRTG 2 cut(s) 624, 735
SalI GTCGAC 1 cut(s) 1092
SaqAI TTAA 2 cut(s) 753, 792
SatI GCNGC 5 cut(s) 201, 612, 888, 891, 1308
Sau3AI GATC 7 cut(s) 106, 290, 988, 1186, 1207, 1222, 1228
Sau96I GGNCC 1 cut(s) 892
ScaI AGTACT 1 cut(s) 217
ScrFI CCNGG 1 cut(s) 1217
SduI GDGCHC 1 cut(s) 565
SfaNI GCATC 4 cut(s) 602, 871, 928, 1157
SfcI CTRYAG 2 cut(s) 198, 1087
SmiMI CAYNNNNRTG 2 cut(s) 624, 735
SpeI ACTAGT 1 cut(s) 218
Sse9I AATT 3 cut(s) 226, 525, 542
SseBI AGGCCT 1 cut(s) 96
SsiI CCGC 3 cut(s) 580, 890, 1312
SspMI CTAG 8 cut(s) 102, 219, 255, 276, 720, 846, 884, 984
StuI AGGCCT 1 cut(s) 96
StyD4I CCNGG 1 cut(s) 1215
StyI CCWWGG 2 cut(s) 57, 983
TaaI ACNGT 2 cut(s) 1029, 1122
TaiI ACGT 3 cut(s) 790, 826, 909
TaqI TCGA 3 cut(s) 39, 289, 1093
TasI AATT 3 cut(s) 226, 525, 542
TatI WGTACW 1 cut(s) 215
TauI GCSGC 1 cut(s) 893
TfiI GAWTC 2 cut(s) 179, 872
Tru1I TTAA 2 cut(s) 753, 792
Tru9I TTAA 2 cut(s) 753, 792
TscAI CASTG 2 cut(s) 595, 1306
TseFI GTSAC 3 cut(s) 958, 1023, 1134
TseI GCWGC 4 cut(s) 200, 611, 887, 1307
Tsp45I GTSAC 3 cut(s) 958, 1023, 1134
TspDTI ATGAA 4 cut(s) 665, 816, 864, 990
TspRI CASTG 2 cut(s) 595, 1306
Van91I CCANNNNNTGG 1 cut(s) 1250
XapI RAATTY 1 cut(s) 542
XceI RCATGY 2 cut(s) 734, 854
XmaJI CCTAGG 1 cut(s) 983
XmiI GTMKAC 1 cut(s) 1093
XmnI GAANNNNTTC 2 cut(s) 285, 672
XspI CTAG 8 cut(s) 102, 219, 255, 276, 720, 846, 884, 984
ZrmI AGTACT 1 cut(s) 217
Zsp2I ATGCAT 2 cut(s) 742, 943
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.