pycom14g12210

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0000847
Physical Location & Seq
Reverse (-)
15251819 .. 15253626
1808 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g12210.2

Sequence Viewer

Length: 1011 bp
ATGTGCAATGGAAGAACAAAACCGGAATGCTACCTATACCGTGTTTTTGGACTTCCTGCTGGAAGAAGGGAAGTTATAGAGAGGATTCGGCCAGGCACAAAGCTGTTTCTCTTTGATTATGATGTCAAGTTTCTTTATGGGGTGTATGAAGCAACCACTGCTGGAAAGATGAACTTGGAAAGAACAGCTTTTGGTGGAAGCTTTCCTGCACAGGTAAGATTCAGAATTTACAAGGAATGTCTCCCCTTGCCTGAGAGTGGTTTTAAACATGCTATTCTAGATAACTACCAGAAAGGCTCCAAGAAGTTTAACCCCATTCTCAATTGCAAACAAGTAAGCCATTTGTTATCACTGTTTCATCCTCTTACCACACAATCACCTGTCAAATATGACAATTCCTATATAAAGGATCAATACCCAAGCCTGCCTCCTTCAGGCACTACATATTATAACTCAACGCGTCTCAGTCAAGCCCCACAAGTGTTGTTTCCTCAATATGTTCCACAGGCAGTCCTAGTTCAGCAGTGGGATGGTCGTGGATCCACAGGAAACACGGGTCTTTTCCATCATGCAGACCAACCTGCTGTGCCAAATGCAGGTTCACAATCAGTAATGCCAACTCAATCTACGGAGGAACAGTTTCAGTCCACAGCAACCCTGCCTTCCATGAAGGATCCAAATGCATCTTCGTTATATGGCGGTCTTACTTCACCAATGTTGGAGCCTCAGCCTCAGCCTCAGTATATTCAACCGAGTGTCATACACCAACAGCCTGCTAGCCATGGATACATGTCATACATGGGCTACATTTATCCTGCTCTGCAACCGCAAGCTGTGCCAGCTCCAAATCAGACATATTATTCTGCACAAGTGGATCACAGTTACTCCGCTGAACTACCTGCCTCACAAGCACCCACATCATATGAAAGCTATCAGAGGCACGAGGCCACACATGATGAAGTGCCTACTGATCAACAAACGAGCTTGGGATATGGGTACAATCACCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

337

Amino Acids

37.65

Weight (kDa)

7.17

Isoelectric Point (pI)

59.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 450
Acc36I ACCTGC 3 cut(s) 587, 589, 907
AccII CGCG 1 cut(s) 460
AciI CCGC 3 cut(s) 699, 827, 888
AclWI GGATC 6 cut(s) 417, 534, 547, 668, 681, 882
AcoI YGGCCR 1 cut(s) 89
AcsI RAATTY 1 cut(s) 225
AcuI CTGAAG 1 cut(s) 417
AfaI GTAC 1 cut(s) 998
AfiI CCNNNNNNNGG 3 cut(s) 257, 434, 596
AflIII ACRYGT 2 cut(s) 458, 789
AgsI TTSAA 1 cut(s) 749
AjnI CCWGG 1 cut(s) 91
AjuI GAANNNNNNNTTGG 4 cut(s) 583, 615, 670, 702
AluBI AGCT 7 cut(s) 103, 188, 201, 833, 842, 930, 984
AluI AGCT 7 cut(s) 103, 188, 201, 833, 842, 930, 984
Alw26I GTCTC 2 cut(s) 245, 467
AlwI GGATC 6 cut(s) 417, 534, 547, 668, 681, 882
AoxI GGCC 2 cut(s) 89, 945
ApoI RAATTY 1 cut(s) 225
Asp700I GAANNNNTTC 1 cut(s) 639
AsuHPI GGTGA 3 cut(s) 369, 702, 995
AsuNHI GCTAGC 1 cut(s) 776
BamHI GGATCC 2 cut(s) 539, 673
BauI CACGAG 1 cut(s) 941
BbvCI CCTCAGC 2 cut(s) 726, 732
BccI CCATC 2 cut(s) 524, 573
BciT130I CCWGG 1 cut(s) 93
BciVI GTATCC 1 cut(s) 779
BclI TGATCA 1 cut(s) 970
BcoDI GTCTC 2 cut(s) 245, 467
BfaI CTAG 3 cut(s) 278, 515, 777
BfuAI ACCTGC 3 cut(s) 587, 589, 907
BfuI GTATCC 1 cut(s) 779
Bme1390I CCNGG 1 cut(s) 93
BmiI GGNNCC 4 cut(s) 298, 541, 675, 723
BmrFI CCNGG 1 cut(s) 93
BmsI GCATC 1 cut(s) 692
BmtI GCTAGC 1 cut(s) 780
Bpu10I CCTNAGC 2 cut(s) 726, 732
BsaJI CCNNGG 1 cut(s) 781
BsaWI WCCGGW 1 cut(s) 22
BsaXI ACNNNNNCTCC 4 cut(s) 623, 653, 869, 899
Bsc4I CCNNNNNNNGG 3 cut(s) 257, 434, 596
Bse3DI GCAATG 1 cut(s) 13
BseBI CCWGG 1 cut(s) 93
BseDI CCNNGG 1 cut(s) 781
BseGI GGATG 2 cut(s) 358, 535
BseLI CCNNNNNNNGG 3 cut(s) 257, 434, 596
BseMI GCAATG 1 cut(s) 13
BseMII CTCAG 5 cut(s) 243, 478, 740, 746, 752
BsgI GTGCAG 2 cut(s) 192, 849
Bsh1236I CGCG 1 cut(s) 460
BshFI GGCC 2 cut(s) 91, 947
BsiSI CCGG 1 cut(s) 23
BslI CCNNNNNNNGG 3 cut(s) 257, 434, 596
BsmAI GTCTC 2 cut(s) 245, 467
BsmBI CGTCTC 1 cut(s) 467
BsmI GAATGC 1 cut(s) 32
BsnI GGCC 2 cut(s) 91, 947
Bsp143I GATC 5 cut(s) 409, 539, 673, 874, 970
Bsp19I CCATGG 1 cut(s) 781
BspACI CCGC 3 cut(s) 699, 827, 888
BspANI GGCC 2 cut(s) 91, 947
BspCNI CTCAG 5 cut(s) 244, 477, 739, 745, 751
BspFNI CGCG 1 cut(s) 460
BspLI GGNNCC 4 cut(s) 298, 541, 675, 723
BspMI ACCTGC 3 cut(s) 587, 589, 907
BspOI GCTAGC 1 cut(s) 780
BspPI GGATC 6 cut(s) 417, 534, 547, 668, 681, 882
BsrDI GCAATG 1 cut(s) 13
BssECI CCNNGG 1 cut(s) 781
BssMI GATC 5 cut(s) 409, 539, 673, 874, 970
BssSI CACGAG 1 cut(s) 941
BssT1I CCWWGG 1 cut(s) 781
Bst2BI CACGAG 1 cut(s) 941
Bst2UI CCWGG 1 cut(s) 93
Bst4CI ACNGT 4 cut(s) 41, 354, 639, 881
BstAPI GCANNNNNTGC 2 cut(s) 158, 835
BstC8I GCNNGC 5 cut(s) 425, 774, 778, 831, 840
BstDEI CTNAG 5 cut(s) 252, 464, 726, 732, 738
BstDSI CCRYGG 1 cut(s) 781
BstENI CCTNNNNNAGG 1 cut(s) 432
BstF5I GGATG 2 cut(s) 358, 535
BstFNI CGCG 1 cut(s) 460
BstKTI GATC 5 cut(s) 412, 542, 676, 877, 973
BstMAI GTCTC 2 cut(s) 245, 467
BstMBI GATC 5 cut(s) 409, 539, 673, 874, 970
BstMWI GCNNNNNNNGC 4 cut(s) 158, 835, 839, 908
BstNI CCWGG 1 cut(s) 93
BstNSI RCATGY 2 cut(s) 272, 793
BstSCI CCNGG 1 cut(s) 91
BstUI CGCG 1 cut(s) 460
BstX2I RGATCY 2 cut(s) 539, 673
BstYI RGATCY 2 cut(s) 539, 673
BsuI GTATCC 1 cut(s) 779
BsuRI GGCC 2 cut(s) 91, 947
BtgI CCRYGG 1 cut(s) 781
BtsCI GGATG 2 cut(s) 358, 535
BtsI GCAGTG 2 cut(s) 156, 530
BtsIMutI CAGTG 3 cut(s) 156, 350, 530
BveI ACCTGC 3 cut(s) 587, 589, 907
Cac8I GCNNGC 5 cut(s) 425, 774, 778, 831, 840
CseI GACGC 1 cut(s) 449
Csp6I GTAC 1 cut(s) 997
CspCI CAANNNNNGTGG 2 cut(s) 465, 500
CviAII CATG 7 cut(s) 269, 569, 667, 782, 790, 799, 953
CviQI GTAC 1 cut(s) 997
DdeI CTNAG 5 cut(s) 252, 464, 726, 732, 738
DpnI GATC 5 cut(s) 411, 541, 675, 876, 972
DpnII GATC 5 cut(s) 409, 539, 673, 874, 970
DraI TTTAAA 1 cut(s) 265
EaeI YGGCCR 1 cut(s) 89
Eco130I CCWWGG 1 cut(s) 781
Eco57I CTGAAG 1 cut(s) 417
EcoNI CCTNNNNNAGG 1 cut(s) 432
EcoRII CCWGG 1 cut(s) 91
EcoT14I CCWWGG 1 cut(s) 781
EcoT22I ATGCAT 1 cut(s) 685
ErhI CCWWGG 1 cut(s) 781
Esp3I CGTCTC 1 cut(s) 467
FaeI CATG 7 cut(s) 272, 572, 670, 785, 793, 802, 956
FalI AAGNNNNNCTT 4 cut(s) 158, 190, 172, 204
FatI CATG 7 cut(s) 268, 568, 666, 781, 789, 798, 952
FauNDI CATATG 1 cut(s) 922
FbaI TGATCA 1 cut(s) 970
FokI GGATG 2 cut(s) 345, 542
FspBI CTAG 3 cut(s) 278, 515, 777
HaeIII GGCC 2 cut(s) 91, 947
HapII CCGG 1 cut(s) 23
HgaI GACGC 1 cut(s) 449
Hin1II CATG 7 cut(s) 272, 572, 670, 785, 793, 802, 956
HindIII AAGCTT 1 cut(s) 199
HinfI GANTC 2 cut(s) 85, 219
HpaII CCGG 1 cut(s) 23
HphI GGTGA 3 cut(s) 369, 702, 995
Hpy166II GTNNAC 2 cut(s) 602, 648
Hpy188I TCNGA 3 cut(s) 224, 852, 936
Hpy188III TCNNGA 1 cut(s) 278
Hpy8I GTNNAC 2 cut(s) 602, 648
HpyAV CCTTC 4 cut(s) 60, 441, 664, 672
HpyCH4III ACNGT 4 cut(s) 41, 354, 639, 881
HpyCH4V TGCA 8 cut(s) 6, 209, 327, 572, 596, 683, 823, 866
HpyF10VI GCNNNNNNNGC 4 cut(s) 158, 835, 839, 908
HpyF3I CTNAG 5 cut(s) 252, 464, 726, 732, 738
Hsp92II CATG 7 cut(s) 272, 572, 670, 785, 793, 802, 956
Ksp22I TGATCA 1 cut(s) 970
Kzo9I GATC 5 cut(s) 409, 539, 673, 874, 970
LmnI GCTCC 3 cut(s) 302, 721, 847
LweI GCATC 1 cut(s) 692
MaeI CTAG 3 cut(s) 278, 515, 777
MaeIII GTNAC 1 cut(s) 881
MalI GATC 5 cut(s) 411, 541, 675, 876, 972
MboI GATC 5 cut(s) 409, 539, 673, 874, 970
MboII GAAGA 3 cut(s) 24, 75, 678
MfeI CAATTG 1 cut(s) 322
MflI RGATCY 2 cut(s) 539, 673
MluCI AATT 3 cut(s) 225, 322, 394
MluI ACGCGT 1 cut(s) 458
MmeI TCCRAC 1 cut(s) 699
Mph1103I ATGCAT 1 cut(s) 685
MroXI GAANNNNTTC 1 cut(s) 639
MseI TTAA 2 cut(s) 264, 309
MspA1I CMGCKG 1 cut(s) 890
MspI CCGG 1 cut(s) 23
MspR9I CCNGG 1 cut(s) 93
MunI CAATTG 1 cut(s) 322
Mva1269I GAATGC 1 cut(s) 32
MvaI CCWGG 1 cut(s) 93
MvnI CGCG 1 cut(s) 460
MwoI GCNNNNNNNGC 4 cut(s) 158, 835, 839, 908
NcoI CCATGG 1 cut(s) 781
NdeI CATATG 1 cut(s) 922
NdeII GATC 5 cut(s) 409, 539, 673, 874, 970
NheI GCTAGC 1 cut(s) 776
NlaIII CATG 7 cut(s) 272, 572, 670, 785, 793, 802, 956
NlaIV GGNNCC 4 cut(s) 298, 541, 675, 723
NsiI ATGCAT 1 cut(s) 685
NspI RCATGY 2 cut(s) 272, 793
PciI ACATGT 1 cut(s) 789
PctI GAATGC 1 cut(s) 32
PdmI GAANNNNTTC 1 cut(s) 639
PfeI GAWTC 2 cut(s) 85, 219
PscI ACATGT 1 cut(s) 789
PsiI TTATAA 1 cut(s) 450
Psp6I CCWGG 1 cut(s) 91
PspGI CCWGG 1 cut(s) 91
PspN4I GGNNCC 4 cut(s) 298, 541, 675, 723
PsuI RGATCY 2 cut(s) 539, 673
RsaI GTAC 1 cut(s) 998
RsaNI GTAC 1 cut(s) 997
SaqAI TTAA 2 cut(s) 264, 309
Sau3AI GATC 5 cut(s) 409, 539, 673, 874, 970
ScrFI CCNGG 1 cut(s) 93
SfaNI GCATC 1 cut(s) 692
Sse9I AATT 3 cut(s) 225, 322, 394
SsiI CCGC 3 cut(s) 699, 827, 888
SspMI CTAG 3 cut(s) 278, 515, 777
StyD4I CCNGG 1 cut(s) 91
StyI CCWWGG 1 cut(s) 781
TaaI ACNGT 4 cut(s) 41, 354, 639, 881
TasI AATT 3 cut(s) 225, 322, 394
TfiI GAWTC 2 cut(s) 85, 219
Tru1I TTAA 2 cut(s) 264, 309
Tru9I TTAA 2 cut(s) 264, 309
TscAI CASTG 3 cut(s) 163, 357, 530
TspDTI ATGAA 6 cut(s) 162, 185, 347, 683, 939, 972
TspGWI ACGGA 1 cut(s) 644
TspRI CASTG 3 cut(s) 163, 357, 530
XagI CCTNNNNNAGG 1 cut(s) 432
XapI RAATTY 1 cut(s) 225
XbaI TCTAGA 1 cut(s) 277
XceI RCATGY 2 cut(s) 272, 793
XmnI GAANNNNTTC 1 cut(s) 639
XspI CTAG 3 cut(s) 278, 515, 777
Zsp2I ATGCAT 1 cut(s) 685
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.