RLG00000007116

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000847
Physical Location & Seq
Forward (+)
13713726 .. 13717475
3750 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007116

Sequence Viewer

Length: 1365 bp
ATGCGTTTTATGGAATCGAGAGTAAAAGCAGTGTGTTTTCTTGTCAATCTTTTCACGGCAACCGCTTCAGTCCGCAGTGTCGTCGTCTTCAACCTCCACCACTATTCCACCTCGATTTCCGGCCTATCCATGGGAAAAAGACGAGGCCAGCGTTTAAGCAAAAGAGGCCTAACTAGAGATCATGATGGTAGGATTAGGAGGATAGACAAGAGTTTTACAAAGGAAAAGAAGAAATCTAAGAAGATAACCGAATCTTCCTTGCTTGAGAATGCAGCTGAGCTTAGTAGTAGTGCCAATTCTGCTTCCAATAAAGCAACTTCTTCTAGTCATGCTAATCAAGCTACTAGAGAAAGGCTTCAATCAAAAAACAAAGGGAAGGATAGTGGGCAATCACATTCTGGTTTTATCTTTATGTGTAATGGAAAGACTAAACCCGAATGTTATCAGTATCGCGTTTTTGGGCTGCCAATGGCAAAAGTAGAAGTTGTGAAGACAATCAAACAGGGTACACATCTATTTTTATATGACTTTGACTTGAAGCTCCTTTATGGCACATACAGCGCAACTTCGGATGGAGCATTAGATTTGGAGCCAATTGCATTCAAGGGAAAATTTCCTGCACAGGTCAGGTTTAAGATTTCGAAGGATTGTTTACCGCTTCACGAAAATGTGGCAAGAACAGGCTCATATCCATCTCCTATTAGGGAAGAAGGATTTCATCCAACAGCAAGGTCGTCTCTTCAAGAGGGCTTATATTTATCTAGTGTACAACGTGCTCATGCATTGCCACTTCTTAATACGCAATCCATACAGGTTGTTCCATATCCACGTTTTAAAAAATATGAAGCAGAAAGATGTGTGGCACGTGTTCAGCCTCCCATAGAGCCTCAACATGTTGTTCAGCATCAGCCAGAATCGTATTATCTAGCAGCGGCCCATGAGCCATACATGCCTGAAAAACCTTTCTTGTCCTCTCATGATGCATATAGAAGGCATGGGGTCACACTTCCACAGGAGATGGTTCCTAGGGATCAAGATGTTTCATATGGAAGTGTGTATCGCTTGTCACAGTTGCTGAAGGAGAGAGAAAGAGATATTGTTTCCCATTCCGATTATATTGCTGAAACCTGTAGTCGACACTTGCCAACTCATCCAGCCTCACAGTTTAGGTTGCAGTCACATTCTTCGGCATCATCTTATGAGTTACCATCAACTTACCAACCATATTATCAAGCAACGACCCATCAGGATCAGAACCCGGTGGATTGTGATCCTCTTCAGAGACCATTGCATGGAAGCTCTAATGAGGCAAGTTTGCCAATCTGTTCTCACTTGTCTTCCACTGGAGCTGCCCGCTACTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

455

Amino Acids

51.21

Weight (kDa)

9.44

Isoelectric Point (pI)

46.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 134 - 226 1.1e-36 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1292
AccI GTMKAC 1 cut(s) 1135
AccII CGCG 1 cut(s) 453
AciI CCGC 5 cut(s) 63, 73, 656, 932, 1354
AclWI GGATC 3 cut(s) 1038, 1257, 1265
AcsI RAATTY 1 cut(s) 611
AcuI CTGAAG 3 cut(s) 51, 1097, 1262
AcvI CACGTG 1 cut(s) 866
AfaI GTAC 2 cut(s) 508, 768
AfiI CCNNNNNNNGG 2 cut(s) 130, 1292
AflIII ACRYGT 2 cut(s) 865, 892
AgsI TTSAA 5 cut(s) 91, 359, 538, 604, 743
AluBI AGCT 6 cut(s) 275, 280, 341, 541, 1299, 1349
AluI AGCT 6 cut(s) 275, 280, 341, 541, 1299, 1349
Alw21I GWGCWC 1 cut(s) 778
Alw26I GTCTC 2 cut(s) 741, 1276
AlwI GGATC 3 cut(s) 1038, 1257, 1265
AlwNI CAGNNNCTG 1 cut(s) 1075
AoxI GGCC 4 cut(s) 121, 145, 166, 933
ApeKI GCWGC 4 cut(s) 272, 463, 929, 1349
ApoI RAATTY 1 cut(s) 611
Asp700I GAANNNNTTC 2 cut(s) 354, 714
AspA2I CCTAGG 1 cut(s) 1025
AspLEI GCGC 1 cut(s) 563
AspS9I GGNCC 1 cut(s) 934
AsuC2I CCSGG 1 cut(s) 1259
AsuII TTCGAA 1 cut(s) 641
AvrII CCTAGG 1 cut(s) 1025
BbrPI CACGTG 1 cut(s) 866
BbsI GAAGAC 3 cut(s) 79, 497, 1329
Bbv12I GWGCWC 1 cut(s) 778
BbvI GCAGC 4 cut(s) 284, 450, 941, 1336
BccI CCATC 6 cut(s) 179, 566, 700, 1012, 1216, 1251
BceAI ACGGC 1 cut(s) 72
BcgI CGANNNNNNTGC 4 cut(s) 64, 98, 1100, 1134
BcnI CCSGG 1 cut(s) 1259
BcoDI GTCTC 2 cut(s) 741, 1276
BfaI CTAG 6 cut(s) 174, 324, 345, 762, 926, 1026
BfmI CTRYAG 1 cut(s) 1129
BisI GCNGC 5 cut(s) 273, 464, 930, 933, 1350
BlnI CCTAGG 1 cut(s) 1025
BlpI GCTNAGC 1 cut(s) 276
BlsI GCNGC 5 cut(s) 274, 465, 931, 934, 1351
Bme1390I CCNGG 1 cut(s) 1259
BmgT120I GGNCC 1 cut(s) 934
BmiI GGNNCC 2 cut(s) 591, 1023
BmrFI CCNGG 1 cut(s) 1259
BmsI GCATC 3 cut(s) 913, 970, 1199
BpiI GAAGAC 3 cut(s) 79, 497, 1329
BpmI CTGGAG 1 cut(s) 1365
Bpu1102I GCTNAGC 1 cut(s) 276
Bpu14I TTCGAA 1 cut(s) 641
BpuEI CTTGAG 1 cut(s) 284
BpuMI CCSGG 1 cut(s) 1259
BsaAI YACGTR 1 cut(s) 866
BsaBI GATNNNNATC 1 cut(s) 1269
BsaI GGTCTC 1 cut(s) 1276
BsaJI CCNNGG 2 cut(s) 129, 1025
Bsc4I CCNNNNNNNGG 2 cut(s) 130, 1292
Bse1I ACTGG 1 cut(s) 1348
Bse3DI GCAATG 2 cut(s) 782, 1286
Bse8I GATNNNNATC 1 cut(s) 1269
BseDI CCNNGG 2 cut(s) 129, 1025
BseGI GGATG 3 cut(s) 577, 718, 1150
BseJI GATNNNNATC 1 cut(s) 1269
BseLI CCNNNNNNNGG 2 cut(s) 130, 1292
BseMI GCAATG 2 cut(s) 782, 1286
BseMII CTCAG 1 cut(s) 267
BseNI ACTGG 1 cut(s) 1348
BseXI GCAGC 4 cut(s) 284, 450, 941, 1336
BsgI GTGCAG 1 cut(s) 603
Bsh1236I CGCG 1 cut(s) 453
BshFI GGCC 4 cut(s) 123, 147, 168, 935
BsiHKAI GWGCWC 1 cut(s) 778
BsiSI CCGG 2 cut(s) 120, 1259
BslI CCNNNNNNNGG 2 cut(s) 130, 1292
BsmAI GTCTC 2 cut(s) 741, 1276
BsmBI CGTCTC 1 cut(s) 741
BsmI GAATGC 2 cut(s) 274, 599
BsnI GGCC 4 cut(s) 123, 147, 168, 935
Bso31I GGTCTC 1 cut(s) 1276
Bsp119I TTCGAA 1 cut(s) 641
Bsp1286I GDGCHC 1 cut(s) 778
Bsp1407I TGTACA 1 cut(s) 766
Bsp143I GATC 4 cut(s) 178, 1030, 1249, 1270
Bsp1720I GCTNAGC 1 cut(s) 276
Bsp19I CCATGG 1 cut(s) 129
BspACI CCGC 5 cut(s) 63, 73, 656, 932, 1354
BspANI GGCC 4 cut(s) 123, 147, 168, 935
BspCNI CTCAG 1 cut(s) 268
BspFNI CGCG 1 cut(s) 453
BspHI TCATGA 2 cut(s) 181, 976
BspLI GGNNCC 2 cut(s) 591, 1023
BspPI GGATC 3 cut(s) 1038, 1257, 1265
BspT104I TTCGAA 1 cut(s) 641
BspTNI GGTCTC 1 cut(s) 1276
BsrDI GCAATG 2 cut(s) 782, 1286
BsrGI TGTACA 1 cut(s) 766
BsrI ACTGG 1 cut(s) 1348
BssECI CCNNGG 2 cut(s) 129, 1025
BssMI GATC 4 cut(s) 178, 1030, 1249, 1270
BssT1I CCWWGG 2 cut(s) 129, 1025
Bst4CI ACNGT 2 cut(s) 1071, 1164
Bst6I CTCTTC 2 cut(s) 744, 1281
BstAUI TGTACA 1 cut(s) 766
BstBAI YACGTR 1 cut(s) 866
BstBI TTCGAA 1 cut(s) 641
BstC8I GCNNGC 2 cut(s) 149, 1354
BstDEI CTNAG 3 cut(s) 237, 276, 281
BstDSI CCRYGG 1 cut(s) 129
BstF5I GGATG 3 cut(s) 577, 718, 1150
BstFNI CGCG 1 cut(s) 453
BstHHI GCGC 1 cut(s) 563
BstKTI GATC 4 cut(s) 181, 1033, 1252, 1273
BstMAI GTCTC 2 cut(s) 741, 1276
BstMBI GATC 4 cut(s) 178, 1030, 1249, 1270
BstMWI GCNNNNNNNGC 5 cut(s) 165, 299, 338, 558, 949
BstNSI RCATGY 2 cut(s) 896, 952
BstSCI CCNGG 1 cut(s) 1257
BstSFI CTRYAG 1 cut(s) 1129
BstUI CGCG 1 cut(s) 453
BstV1I GCAGC 4 cut(s) 284, 450, 941, 1336
BstV2I GAAGAC 3 cut(s) 79, 497, 1329
BsuRI GGCC 4 cut(s) 123, 147, 168, 935
BtgI CCRYGG 1 cut(s) 129
BtsCI GGATG 3 cut(s) 577, 718, 1150
BtsI GCAGTG 2 cut(s) 36, 82
BtsIMutI CAGTG 3 cut(s) 36, 82, 1341
Cac8I GCNNGC 2 cut(s) 149, 1354
CaiI CAGNNNCTG 1 cut(s) 1075
CciI TCATGA 2 cut(s) 181, 976
CfoI GCGC 1 cut(s) 563
Cfr13I GGNCC 1 cut(s) 934
Csp6I GTAC 2 cut(s) 507, 767
CviQI GTAC 2 cut(s) 507, 767
DdeI CTNAG 3 cut(s) 237, 276, 281
DpnI GATC 4 cut(s) 180, 1032, 1251, 1272
DpnII GATC 4 cut(s) 178, 1030, 1249, 1270
DraI TTTAAA 1 cut(s) 835
Eam1104I CTCTTC 2 cut(s) 744, 1281
EarI CTCTTC 2 cut(s) 744, 1281
Eco130I CCWWGG 2 cut(s) 129, 1025
Eco147I AGGCCT 1 cut(s) 168
Eco31I GGTCTC 1 cut(s) 1276
Eco57I CTGAAG 3 cut(s) 51, 1097, 1262
Eco72I CACGTG 1 cut(s) 866
EcoT14I CCWWGG 2 cut(s) 129, 1025
EcoT22I ATGCAT 2 cut(s) 784, 985
ErhI CCWWGG 2 cut(s) 129, 1025
Esp3I CGTCTC 1 cut(s) 741
FauI CCCGC 1 cut(s) 1361
FauNDI CATATG 1 cut(s) 1045
FblI GTMKAC 1 cut(s) 1135
Fnu4HI GCNGC 5 cut(s) 273, 464, 930, 933, 1350
FokI GGATG 3 cut(s) 584, 705, 1137
Fsp4HI GCNGC 5 cut(s) 273, 464, 930, 933, 1350
FspBI CTAG 6 cut(s) 174, 324, 345, 762, 926, 1026
GlaI GCGC 1 cut(s) 562
GluI GCNGC 5 cut(s) 273, 464, 930, 933, 1350
GsuI CTGGAG 1 cut(s) 1365
HaeIII GGCC 4 cut(s) 123, 147, 168, 935
HapII CCGG 2 cut(s) 120, 1259
HhaI GCGC 1 cut(s) 563
Hin6I GCGC 1 cut(s) 561
HinP1I GCGC 1 cut(s) 561
HincII GTYRAC 1 cut(s) 1136
HindII GTYRAC 1 cut(s) 1136
HinfI GANTC 3 cut(s) 14, 251, 914
HpaII CCGG 2 cut(s) 120, 1259
Hpy166II GTNNAC 4 cut(s) 509, 653, 767, 1136
Hpy188I TCNGA 4 cut(s) 571, 1111, 1254, 1281
Hpy188III TCNNGA 7 cut(s) 18, 182, 662, 743, 977, 1034, 1247
Hpy8I GTNNAC 4 cut(s) 509, 653, 767, 1136
Hpy99I CGWCG 1 cut(s) 86
HpyAV CCTTC 5 cut(s) 370, 637, 704, 984, 1072
HpyCH4III ACNGT 2 cut(s) 1071, 1164
HpyCH4IV ACGT 3 cut(s) 772, 829, 865
HpyCH4V TGCA 7 cut(s) 272, 599, 620, 782, 983, 1174, 1291
HpyF10VI GCNNNNNNNGC 5 cut(s) 165, 299, 338, 558, 949
HpyF3I CTNAG 3 cut(s) 237, 276, 281
HpySE526I ACGT 3 cut(s) 772, 829, 865
HspAI GCGC 1 cut(s) 561
Kzo9I GATC 4 cut(s) 178, 1030, 1249, 1270
LmnI GCTCC 4 cut(s) 546, 575, 589, 1346
Lsp1109I GCAGC 4 cut(s) 284, 450, 941, 1336
LweI GCATC 3 cut(s) 913, 970, 1199
MaeI CTAG 6 cut(s) 174, 324, 345, 762, 926, 1026
MaeII ACGT 3 cut(s) 772, 829, 865
MaeIII GTNAC 4 cut(s) 1000, 1065, 1176, 1203
MalI GATC 4 cut(s) 180, 1032, 1251, 1272
MboI GATC 4 cut(s) 178, 1030, 1249, 1270
MfeI CAATTG 1 cut(s) 594
MhlI GDGCHC 1 cut(s) 778
MluCI AATT 3 cut(s) 295, 594, 611
MmeI TCCRAC 1 cut(s) 746
Mph1103I ATGCAT 2 cut(s) 784, 985
MroXI GAANNNNTTC 2 cut(s) 354, 714
MseI TTAA 4 cut(s) 155, 633, 795, 834
MslI CAYNNNNRTG 1 cut(s) 666
MspA1I CMGCKG 2 cut(s) 275, 932
MspI CCGG 2 cut(s) 120, 1259
MspR9I CCNGG 1 cut(s) 1259
MunI CAATTG 1 cut(s) 594
Mva1269I GAATGC 2 cut(s) 274, 599
MvnI CGCG 1 cut(s) 453
MwoI GCNNNNNNNGC 5 cut(s) 165, 299, 338, 558, 949
NciI CCSGG 1 cut(s) 1259
NcoI CCATGG 1 cut(s) 129
NdeI CATATG 1 cut(s) 1045
NdeII GATC 4 cut(s) 178, 1030, 1249, 1270
NlaIV GGNNCC 2 cut(s) 591, 1023
NmuCI GTSAC 3 cut(s) 1000, 1065, 1176
NsiI ATGCAT 2 cut(s) 784, 985
NspI RCATGY 2 cut(s) 896, 952
NspV TTCGAA 1 cut(s) 641
PagI TCATGA 2 cut(s) 181, 976
PceI AGGCCT 1 cut(s) 168
PciI ACATGT 1 cut(s) 892
PcsI WCGNNNNNNNCGW 1 cut(s) 148
PctI GAATGC 2 cut(s) 274, 599
PdmI GAANNNNTTC 2 cut(s) 354, 714
PfeI GAWTC 3 cut(s) 14, 251, 914
PflMI CCANNNNNTGG 1 cut(s) 1292
PkrI GCNGC 5 cut(s) 274, 465, 931, 934, 1351
PmaCI CACGTG 1 cut(s) 866
PmlI CACGTG 1 cut(s) 866
Ppu21I YACGTR 1 cut(s) 866
PscI ACATGT 1 cut(s) 892
PspCI CACGTG 1 cut(s) 866
PspN4I GGNNCC 2 cut(s) 591, 1023
PspPI GGNCC 1 cut(s) 934
PstNI CAGNNNCTG 1 cut(s) 1075
PvuII CAGCTG 1 cut(s) 275
RsaI GTAC 2 cut(s) 508, 768
RsaNI GTAC 2 cut(s) 507, 767
RseI CAYNNNNRTG 1 cut(s) 666
SalI GTCGAC 1 cut(s) 1134
SaqAI TTAA 4 cut(s) 155, 633, 795, 834
SatI GCNGC 5 cut(s) 273, 464, 930, 933, 1350
Sau3AI GATC 4 cut(s) 178, 1030, 1249, 1270
Sau96I GGNCC 1 cut(s) 934
ScrFI CCNGG 1 cut(s) 1259
SduI GDGCHC 1 cut(s) 778
SfaNI GCATC 3 cut(s) 913, 970, 1199
SfcI CTRYAG 1 cut(s) 1129
SfuI TTCGAA 1 cut(s) 641
SmiMI CAYNNNNRTG 1 cut(s) 666
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
Sse9I AATT 3 cut(s) 295, 594, 611
SseBI AGGCCT 1 cut(s) 168
SsiI CCGC 5 cut(s) 63, 73, 656, 932, 1354
SspMI CTAG 6 cut(s) 174, 324, 345, 762, 926, 1026
StuI AGGCCT 1 cut(s) 168
StyD4I CCNGG 1 cut(s) 1257
StyI CCWWGG 2 cut(s) 129, 1025
TaaI ACNGT 2 cut(s) 1071, 1164
TaiI ACGT 3 cut(s) 775, 832, 868
TaqI TCGA 4 cut(s) 17, 113, 641, 1135
TasI AATT 3 cut(s) 295, 594, 611
TatI WGTACW 1 cut(s) 766
TauI GCSGC 1 cut(s) 935
TfiI GAWTC 3 cut(s) 14, 251, 914
Tru1I TTAA 4 cut(s) 155, 633, 795, 834
Tru9I TTAA 4 cut(s) 155, 633, 795, 834
TscAI CASTG 3 cut(s) 36, 82, 1348
TseFI GTSAC 3 cut(s) 1000, 1065, 1176
TseI GCWGC 4 cut(s) 272, 463, 929, 1349
Tsp45I GTSAC 3 cut(s) 1000, 1065, 1176
TspDTI ATGAA 3 cut(s) 707, 858, 1032
TspRI CASTG 3 cut(s) 36, 82, 1348
Van91I CCANNNNNTGG 1 cut(s) 1292
XapI RAATTY 1 cut(s) 611
XceI RCATGY 2 cut(s) 896, 952
XmaJI CCTAGG 1 cut(s) 1025
XmiI GTMKAC 1 cut(s) 1135
XmnI GAANNNNTTC 2 cut(s) 354, 714
XspI CTAG 6 cut(s) 174, 324, 345, 762, 926, 1026
Zsp2I ATGCAT 2 cut(s) 784, 985
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.