Rmu_sc0008612.1_g000009

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008612.1
OG0000847
Physical Location & Seq
Reverse (-)
33835 .. 38591
4757 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008612.1_g000009.1.cds

Sequence Viewer

Length: 1548 bp
atgctcacgagggcatcttgggtttccgtgttgttcaagcctctgcagttccaagaaataatcttgaaggcctgcatgaggaagaggaaagtactggccatcaccggcctgagtcgtcttcaacctccaccactattcgacctcgatttccggcctatatccatgggaaaaagacgaggcaagcgtctaagcaaaagaggcctaactagagatcatgatggtaggattaggaggatagacaagagttttacaaaggaaaagaagaaatctaagaagataaccgaatcttccttgcctcagactgcagctgagcttagtagtagcactagtgccaattctgcttccaataaagcaacttcttctggtcatgctaatcaagctactagagaaaggattcgatcaaaaaacaaagggaaggatagtgggcaatcacattctggttttatatttatgtgtaatggaaagactaaaccagaatgttatcagtatcgcgtttttgggctaccaatggcaaaagtagaagttgtgaaggcaatcaaacagggtacacatctatttttatatgactttgacttgaagctcctttatggcacatacagcgcaacttcggatggagcattagatttggagccaattgcgttcaagggaaaatttcctgcacaggtcaggtttaagatttccaaggattgtttaccacttcacgaaagttcttttaaaacagccataagagacaattaccagggcggtggtaagtttaaacaagagctgaataacaaacaggtgcgtgctctaatctcattgttccgcccaatcactgcatcaatgcctacttctgctgcccatcaccgagatgtggcaagaacaggctcatatccatctcctattagggaagaaggatttcatccaacagcaaggccgtctcttcaagagggcttatatttatctagcgtacaacatgctcatgcattgccacttcttaatacgcaatccatacaggttgttccatatccacgttttaaaaaatatgaagcagaaagatgtgtggcacgtgttcagcctcccatagagcctagacatgttgttcagcatcagccagattcatattatctagcagcggcccatgagccatacgtgcctgaaaaacctttcttgtcctcccatgatgcatatagaaggcatggggtcacacttccacaggagatggttcctagggatcaagatgtttcatatggaagtgtgtatcgcttgtcacagttgctgaaggagagagaaagagatattgtttcccattccgattatattgctgaaacctatagtcgacacttgccaactcatccagcctcacagtttaggttgcagtcacattcttcggcatcatcttatgagttaccatcaacttaccaaccatatgatcaagcaacgacccatcaggatcagaacccggtggatcgtgatcctcttcagagaccattgcatggaagctctaatgaggcaagtttgccaatttgttttcacttgtcttccactggagctgcccgctactactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

515

Amino Acids

58.25

Weight (kDa)

9.79

Isoelectric Point (pI)

45.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1475
AccI GTMKAC 1 cut(s) 1318
AccII CGCG 1 cut(s) 492
AciI CCGC 4 cut(s) 744, 805, 1115, 1537
AclWI GGATC 4 cut(s) 1221, 1440, 1448, 1455
AcoI YGGCCR 1 cut(s) 96
AcsI RAATTY 1 cut(s) 650
AcuI CTGAAG 2 cut(s) 1280, 1445
AcvI CACGTG 1 cut(s) 1049
AfaI GTAC 3 cut(s) 93, 547, 951
AfiI CCNNNNNNNGG 3 cut(s) 78, 853, 1475
AflIII ACRYGT 2 cut(s) 1048, 1075
AgsI TTSAA 6 cut(s) 37, 67, 122, 577, 643, 926
AhlI ACTAGT 1 cut(s) 326
AjnI CCWGG 1 cut(s) 738
AluBI AGCT 7 cut(s) 308, 313, 380, 580, 766, 1482, 1532
AluI AGCT 7 cut(s) 308, 313, 380, 580, 766, 1482, 1532
Alw21I GWGCWC 1 cut(s) 790
Alw26I GTCTC 3 cut(s) 723, 924, 1459
AlwI GGATC 4 cut(s) 1221, 1440, 1448, 1455
AlwNI CAGNNNCTG 1 cut(s) 1258
AoxI GGCC 7 cut(s) 69, 96, 106, 152, 199, 914, 1116
ApeKI GCWGC 4 cut(s) 305, 836, 1112, 1532
ApoI RAATTY 1 cut(s) 650
Asp700I GAANNNNTTC 2 cut(s) 393, 897
AspA2I CCTAGG 1 cut(s) 1208
AspLEI GCGC 1 cut(s) 602
AspS9I GGNCC 1 cut(s) 1117
AsuC2I CCSGG 1 cut(s) 1442
AsuHPI GGTGA 2 cut(s) 94, 836
AvrII CCTAGG 1 cut(s) 1208
BalI TGGCCA 1 cut(s) 98
BauI CACGAG 1 cut(s) 7
BbrPI CACGTG 1 cut(s) 1049
BbsI GAAGAC 2 cut(s) 110, 1512
Bbv12I GWGCWC 1 cut(s) 790
BbvI GCAGC 4 cut(s) 317, 823, 1124, 1519
BccI CCATC 8 cut(s) 107, 212, 605, 849, 883, 1195, 1399, 1434
BceAI ACGGC 1 cut(s) 901
BcgI CGANNNNNNTGC 2 cut(s) 1283, 1317
BciT130I CCWGG 1 cut(s) 740
BclI TGATCA 1 cut(s) 1411
BcnI CCSGG 1 cut(s) 1442
BcoDI GTCTC 3 cut(s) 723, 924, 1459
BcuI ACTAGT 1 cut(s) 326
BfaI CTAG 7 cut(s) 207, 327, 384, 945, 1071, 1109, 1209
BfmI CTRYAG 3 cut(s) 44, 303, 1312
BisI GCNGC 5 cut(s) 306, 837, 1113, 1116, 1533
BlnI CCTAGG 1 cut(s) 1208
BlpI GCTNAGC 1 cut(s) 309
BlsI GCNGC 5 cut(s) 307, 838, 1114, 1117, 1534
BmcAI AGTACT 1 cut(s) 93
Bme1390I CCNGG 2 cut(s) 740, 1442
BmgT120I GGNCC 1 cut(s) 1117
BmiI GGNNCC 2 cut(s) 630, 1206
BmrFI CCNGG 2 cut(s) 740, 1442
BmsI GCATC 5 cut(s) 23, 827, 1096, 1153, 1382
BpiI GAAGAC 2 cut(s) 110, 1512
BpmI CTGGAG 1 cut(s) 1548
Bpu1102I GCTNAGC 1 cut(s) 309
BpuMI CCSGG 1 cut(s) 1442
BsaAI YACGTR 2 cut(s) 1049, 1132
BsaBI GATNNNNATC 1 cut(s) 1452
BsaI GGTCTC 1 cut(s) 1459
BsaJI CCNNGG 4 cut(s) 162, 681, 739, 1208
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 853, 1475
Bse118I RCCGGY 1 cut(s) 104
Bse1I ACTGG 2 cut(s) 99, 1531
Bse3DI GCAATG 2 cut(s) 965, 1469
Bse8I GATNNNNATC 1 cut(s) 1452
BseBI CCWGG 1 cut(s) 740
BseDI CCNNGG 4 cut(s) 162, 681, 739, 1208
BseGI GGATG 3 cut(s) 616, 901, 1333
BseJI GATNNNNATC 1 cut(s) 1452
BseLI CCNNNNNNNGG 3 cut(s) 78, 853, 1475
BseMI GCAATG 2 cut(s) 965, 1469
BseMII CTCAG 3 cut(s) 101, 300, 311
BseNI ACTGG 2 cut(s) 99, 1531
BseXI GCAGC 4 cut(s) 317, 823, 1124, 1519
BsgI GTGCAG 1 cut(s) 642
Bsh1236I CGCG 1 cut(s) 492
BshFI GGCC 7 cut(s) 71, 98, 108, 154, 201, 916, 1118
BsiHKAI GWGCWC 1 cut(s) 790
BsiSI CCGG 3 cut(s) 105, 151, 1442
BslI CCNNNNNNNGG 3 cut(s) 78, 853, 1475
BsmAI GTCTC 3 cut(s) 723, 924, 1459
BsmBI CGTCTC 1 cut(s) 924
BsnI GGCC 7 cut(s) 71, 98, 108, 154, 201, 916, 1118
Bso31I GGTCTC 1 cut(s) 1459
Bsp1286I GDGCHC 1 cut(s) 790
Bsp143I GATC 7 cut(s) 211, 398, 1213, 1411, 1432, 1447, 1453
Bsp1720I GCTNAGC 1 cut(s) 309
Bsp19I CCATGG 1 cut(s) 162
BspACI CCGC 4 cut(s) 744, 805, 1115, 1537
BspANI GGCC 7 cut(s) 71, 98, 108, 154, 201, 916, 1118
BspCNI CTCAG 3 cut(s) 102, 301, 310
BspFNI CGCG 1 cut(s) 492
BspHI TCATGA 1 cut(s) 214
BspLI GGNNCC 2 cut(s) 630, 1206
BspMAI CTGCAG 2 cut(s) 48, 307
BspPI GGATC 4 cut(s) 1221, 1440, 1448, 1455
BspTNI GGTCTC 1 cut(s) 1459
BsrDI GCAATG 2 cut(s) 965, 1469
BsrFI RCCGGY 1 cut(s) 104
BsrI ACTGG 2 cut(s) 99, 1531
BssAI RCCGGY 1 cut(s) 104
BssECI CCNNGG 4 cut(s) 162, 681, 739, 1208
BssMI GATC 7 cut(s) 211, 398, 1213, 1411, 1432, 1447, 1453
BssSI CACGAG 1 cut(s) 7
BssT1I CCWWGG 3 cut(s) 162, 681, 1208
Bst2BI CACGAG 1 cut(s) 7
Bst2UI CCWGG 1 cut(s) 740
Bst4CI ACNGT 2 cut(s) 1254, 1347
Bst6I CTCTTC 3 cut(s) 77, 927, 1464
BstBAI YACGTR 2 cut(s) 1049, 1132
BstC8I GCNNGC 4 cut(s) 73, 182, 786, 1537
BstDEI CTNAG 6 cut(s) 110, 188, 270, 297, 309, 314
BstDSI CCRYGG 1 cut(s) 162
BstENI CCTNNNNNAGG 1 cut(s) 76
BstF5I GGATG 3 cut(s) 616, 901, 1333
BstFNI CGCG 1 cut(s) 492
BstHHI GCGC 1 cut(s) 602
BstKTI GATC 7 cut(s) 214, 401, 1216, 1414, 1435, 1450, 1456
BstMAI GTCTC 3 cut(s) 723, 924, 1459
BstMBI GATC 7 cut(s) 211, 398, 1213, 1411, 1432, 1447, 1453
BstMWI GCNNNNNNNGC 5 cut(s) 198, 338, 377, 597, 1132
BstNI CCWGG 1 cut(s) 740
BstNSI RCATGY 2 cut(s) 959, 1079
BstSCI CCNGG 2 cut(s) 738, 1440
BstSFI CTRYAG 3 cut(s) 44, 303, 1312
BstUI CGCG 1 cut(s) 492
BstV1I GCAGC 4 cut(s) 317, 823, 1124, 1519
BstV2I GAAGAC 2 cut(s) 110, 1512
BstXI CCANNNNNNTGG 1 cut(s) 746
BsuRI GGCC 7 cut(s) 71, 98, 108, 154, 201, 916, 1118
BtgI CCRYGG 1 cut(s) 162
BtsCI GGATG 3 cut(s) 616, 901, 1333
BtsI GCAGTG 1 cut(s) 813
BtsIMutI CAGTG 2 cut(s) 813, 1524
Cac8I GCNNGC 4 cut(s) 73, 182, 786, 1537
CaiI CAGNNNCTG 1 cut(s) 1258
CciI TCATGA 1 cut(s) 214
CfoI GCGC 1 cut(s) 602
Cfr10I RCCGGY 1 cut(s) 104
Cfr13I GGNCC 1 cut(s) 1117
CseI GACGC 1 cut(s) 173
Csp6I GTAC 3 cut(s) 92, 546, 950
CviQI GTAC 3 cut(s) 92, 546, 950
DdeI CTNAG 6 cut(s) 110, 188, 270, 297, 309, 314
DpnI GATC 7 cut(s) 213, 400, 1215, 1413, 1434, 1449, 1455
DpnII GATC 7 cut(s) 211, 398, 1213, 1411, 1432, 1447, 1453
DraI TTTAAA 3 cut(s) 715, 757, 1018
EaeI YGGCCR 1 cut(s) 96
Eam1104I CTCTTC 3 cut(s) 77, 927, 1464
EarI CTCTTC 3 cut(s) 77, 927, 1464
EciI GGCGGA 1 cut(s) 794
Eco130I CCWWGG 3 cut(s) 162, 681, 1208
Eco147I AGGCCT 2 cut(s) 71, 201
Eco31I GGTCTC 1 cut(s) 1459
Eco57I CTGAAG 2 cut(s) 1280, 1445
Eco72I CACGTG 1 cut(s) 1049
EcoNI CCTNNNNNAGG 1 cut(s) 76
EcoRII CCWGG 1 cut(s) 738
EcoT14I CCWWGG 3 cut(s) 162, 681, 1208
EcoT22I ATGCAT 2 cut(s) 967, 1168
ErhI CCWWGG 3 cut(s) 162, 681, 1208
Esp3I CGTCTC 1 cut(s) 924
FauI CCCGC 1 cut(s) 1544
FauNDI CATATG 2 cut(s) 1228, 1408
FbaI TGATCA 1 cut(s) 1411
FblI GTMKAC 1 cut(s) 1318
Fnu4HI GCNGC 5 cut(s) 306, 837, 1113, 1116, 1533
FokI GGATG 3 cut(s) 623, 888, 1320
Fsp4HI GCNGC 5 cut(s) 306, 837, 1113, 1116, 1533
FspBI CTAG 7 cut(s) 207, 327, 384, 945, 1071, 1109, 1209
GlaI GCGC 1 cut(s) 601
GluI GCNGC 5 cut(s) 306, 837, 1113, 1116, 1533
GsuI CTGGAG 1 cut(s) 1548
HaeIII GGCC 7 cut(s) 71, 98, 108, 154, 201, 916, 1118
HapII CCGG 3 cut(s) 105, 151, 1442
HgaI GACGC 1 cut(s) 173
HhaI GCGC 1 cut(s) 602
Hin6I GCGC 1 cut(s) 600
HinP1I GCGC 1 cut(s) 600
HincII GTYRAC 1 cut(s) 1319
HindII GTYRAC 1 cut(s) 1319
HinfI GANTC 4 cut(s) 112, 284, 394, 1097
HpaII CCGG 3 cut(s) 105, 151, 1442
HphI GGTGA 2 cut(s) 94, 836
Hpy166II GTNNAC 3 cut(s) 548, 692, 1319
Hpy188I TCNGA 5 cut(s) 300, 610, 1294, 1437, 1464
Hpy188III TCNNGA 8 cut(s) 7, 64, 215, 701, 926, 1217, 1430, 1451
Hpy8I GTNNAC 3 cut(s) 548, 692, 1319
HpyAV CCTTC 6 cut(s) 61, 409, 523, 887, 1167, 1255
HpyCH4III ACNGT 2 cut(s) 1254, 1347
HpyCH4IV ACGT 3 cut(s) 1012, 1048, 1131
HpyCH4V TGCA 9 cut(s) 46, 75, 305, 659, 818, 965, 1166, 1357, 1474
HpyF10VI GCNNNNNNNGC 5 cut(s) 198, 338, 377, 597, 1132
HpyF3I CTNAG 6 cut(s) 110, 188, 270, 297, 309, 314
HpySE526I ACGT 3 cut(s) 1012, 1048, 1131
HspAI GCGC 1 cut(s) 600
Ksp22I TGATCA 1 cut(s) 1411
Kzo9I GATC 7 cut(s) 211, 398, 1213, 1411, 1432, 1447, 1453
LmnI GCTCC 4 cut(s) 585, 614, 628, 1529
Lsp1109I GCAGC 4 cut(s) 317, 823, 1124, 1519
LweI GCATC 5 cut(s) 23, 827, 1096, 1153, 1382
MaeI CTAG 7 cut(s) 207, 327, 384, 945, 1071, 1109, 1209
MaeII ACGT 3 cut(s) 1012, 1048, 1131
MaeIII GTNAC 4 cut(s) 1183, 1248, 1359, 1386
MalI GATC 7 cut(s) 213, 400, 1215, 1413, 1434, 1449, 1455
MboI GATC 7 cut(s) 211, 398, 1213, 1411, 1432, 1447, 1453
MfeI CAATTG 1 cut(s) 633
MhlI GDGCHC 1 cut(s) 790
MlsI TGGCCA 1 cut(s) 98
MluCI AATT 5 cut(s) 334, 633, 650, 733, 1503
MluNI TGGCCA 1 cut(s) 98
MlyI GAGTC 1 cut(s) 121
MmeI TCCRAC 1 cut(s) 929
Mox20I TGGCCA 1 cut(s) 98
Mph1103I ATGCAT 2 cut(s) 967, 1168
MroXI GAANNNNTTC 2 cut(s) 393, 897
MscI TGGCCA 1 cut(s) 98
MseI TTAA 5 cut(s) 672, 714, 756, 978, 1017
MslI CAYNNNNRTG 2 cut(s) 849, 960
Msp20I TGGCCA 1 cut(s) 98
MspA1I CMGCKG 2 cut(s) 308, 1115
MspI CCGG 3 cut(s) 105, 151, 1442
MspR9I CCNGG 2 cut(s) 740, 1442
MssI GTTTAAAC 1 cut(s) 757
MunI CAATTG 1 cut(s) 633
MvaI CCWGG 1 cut(s) 740
MvnI CGCG 1 cut(s) 492
MwoI GCNNNNNNNGC 5 cut(s) 198, 338, 377, 597, 1132
NciI CCSGG 1 cut(s) 1442
NcoI CCATGG 1 cut(s) 162
NdeI CATATG 2 cut(s) 1228, 1408
NdeII GATC 7 cut(s) 211, 398, 1213, 1411, 1432, 1447, 1453
NlaIV GGNNCC 2 cut(s) 630, 1206
NmuCI GTSAC 3 cut(s) 1183, 1248, 1359
NsiI ATGCAT 2 cut(s) 967, 1168
NspI RCATGY 2 cut(s) 959, 1079
PagI TCATGA 1 cut(s) 214
PceI AGGCCT 2 cut(s) 71, 201
PciI ACATGT 1 cut(s) 1075
PcsI WCGNNNNNNNCGW 1 cut(s) 181
PdmI GAANNNNTTC 2 cut(s) 393, 897
PfeI GAWTC 3 cut(s) 284, 394, 1097
PflMI CCANNNNNTGG 1 cut(s) 1475
PkrI GCNGC 5 cut(s) 307, 838, 1114, 1117, 1534
PleI GAGTC 1 cut(s) 120
PmaCI CACGTG 1 cut(s) 1049
PmeI GTTTAAAC 1 cut(s) 757
PmlI CACGTG 1 cut(s) 1049
PpsI GAGTC 1 cut(s) 120
Ppu21I YACGTR 2 cut(s) 1049, 1132
PscI ACATGT 1 cut(s) 1075
Psp6I CCWGG 1 cut(s) 738
PspCI CACGTG 1 cut(s) 1049
PspGI CCWGG 1 cut(s) 738
PspN4I GGNNCC 2 cut(s) 630, 1206
PspPI GGNCC 1 cut(s) 1117
PstI CTGCAG 2 cut(s) 48, 307
PstNI CAGNNNCTG 1 cut(s) 1258
PvuII CAGCTG 1 cut(s) 308
RsaI GTAC 3 cut(s) 93, 547, 951
RsaNI GTAC 3 cut(s) 92, 546, 950
RseI CAYNNNNRTG 2 cut(s) 849, 960
SalI GTCGAC 1 cut(s) 1317
SaqAI TTAA 5 cut(s) 672, 714, 756, 978, 1017
SatI GCNGC 5 cut(s) 306, 837, 1113, 1116, 1533
Sau3AI GATC 7 cut(s) 211, 398, 1213, 1411, 1432, 1447, 1453
Sau96I GGNCC 1 cut(s) 1117
ScaI AGTACT 1 cut(s) 93
SchI GAGTC 1 cut(s) 121
ScrFI CCNGG 2 cut(s) 740, 1442
SduI GDGCHC 1 cut(s) 790
SfaNI GCATC 5 cut(s) 23, 827, 1096, 1153, 1382
SfcI CTRYAG 3 cut(s) 44, 303, 1312
SmiMI CAYNNNNRTG 2 cut(s) 849, 960
SpeI ACTAGT 1 cut(s) 326
Sse9I AATT 5 cut(s) 334, 633, 650, 733, 1503
SseBI AGGCCT 2 cut(s) 71, 201
SsiI CCGC 4 cut(s) 744, 805, 1115, 1537
SspMI CTAG 7 cut(s) 207, 327, 384, 945, 1071, 1109, 1209
StuI AGGCCT 2 cut(s) 71, 201
StyD4I CCNGG 2 cut(s) 738, 1440
StyI CCWWGG 3 cut(s) 162, 681, 1208
TaaI ACNGT 2 cut(s) 1254, 1347
TaiI ACGT 3 cut(s) 1015, 1051, 1134
TaqI TCGA 4 cut(s) 138, 144, 397, 1318
TasI AATT 5 cut(s) 334, 633, 650, 733, 1503
TatI WGTACW 1 cut(s) 91
TauI GCSGC 1 cut(s) 1118
TfiI GAWTC 3 cut(s) 284, 394, 1097
Tru1I TTAA 5 cut(s) 672, 714, 756, 978, 1017
Tru9I TTAA 5 cut(s) 672, 714, 756, 978, 1017
TscAI CASTG 2 cut(s) 820, 1531
TseFI GTSAC 3 cut(s) 1183, 1248, 1359
TseI GCWGC 4 cut(s) 305, 836, 1112, 1532
Tsp45I GTSAC 3 cut(s) 1183, 1248, 1359
TspDTI ATGAA 4 cut(s) 890, 1041, 1089, 1215
TspGWI ACGGA 1 cut(s) 16
TspRI CASTG 2 cut(s) 820, 1531
Van91I CCANNNNNTGG 1 cut(s) 1475
XagI CCTNNNNNAGG 1 cut(s) 76
XapI RAATTY 1 cut(s) 650
XceI RCATGY 2 cut(s) 959, 1079
XmaJI CCTAGG 1 cut(s) 1208
XmiI GTMKAC 1 cut(s) 1318
XmnI GAANNNNTTC 2 cut(s) 393, 897
XspI CTAG 7 cut(s) 207, 327, 384, 945, 1071, 1109, 1209
ZrmI AGTACT 1 cut(s) 93
Zsp2I ATGCAT 2 cut(s) 967, 1168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.