FvH4_5g10230

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001380
Physical Location & Seq
Reverse (-)
5847914 .. 5848501
588 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g10230.t1

Sequence Viewer

Length: 588 bp
ATGAAGACGATTCTGTCCTCGGAGACCATGGACATCCCTGACGGCGTCACCGTGAAGGTGAAGGCCAAGGTCATCGAGGTCGAAGGCCCCCGCGGCAAGCTGACCCGCAACTTCAAGCACCTCAACCTCGACTTCGAGCTCATCACCGACGAGTCCACCGGGAAGAAGAAGCTCAAAATCGAGGCCTGGTTTGGCACTCGCAAGACCAGCGCCGCCATCCGCACCGCCCTCAGCCACGTCGACAACCTCATCACCGGCGTCACCAAAGGCTACCGCTACAAGATGCGATTCGTCTACGCTCACTTTCCGATCAACGCCTCCATCCCCAGTTCCAACAACGCCATCGAGATCCGCAACTTTCTCGGCGAGAAGCGAGTCCGGAAGGTGGAGATGCTCAACGGTGTGACGATCACTCGGTCGGAGAAGGTGAAGGATGAGCTGGTTTTGGACGGGAATGACATTGAGTTGGTTTCGAGGTCGGCGGCGCTGATCAATCAGAAATGCCATGTGAAGAACAAGGACATTAGGAAGTTTCTGGATGGGATCTACGTCAGCGAGAGGAGCACCATTGCCACCGGAGACGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L6

Protein Analysis

196

Amino Acids

21.95

Weight (kDa)

9.6

Isoelectric Point (pI)

26.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L6 PF00347 12 - 90 5.1e-11 Ribosomal protein L6
Ribosomal_L6 PF00347 114 - 181 1.2e-12 Ribosomal protein L6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 13
AccI GTMKAC 2 cut(s) 240, 294
AccII CGCG 1 cut(s) 93
AccIII TCCGGA 1 cut(s) 378
AciI CCGC 9 cut(s) 91, 93, 106, 213, 220, 225, 274, 352, 482
AclWI GGATC 2 cut(s) 343, 551
AcyI GRCGYC 2 cut(s) 45, 258
AfiI CCNNNNNNNGG 1 cut(s) 385
AgsI TTSAA 1 cut(s) 115
AjiI CACGTC 1 cut(s) 238
AjnI CCWGG 1 cut(s) 185
AluBI AGCT 4 cut(s) 100, 139, 172, 439
AluI AGCT 4 cut(s) 100, 139, 172, 439
Alw21I GWGCWC 2 cut(s) 141, 566
Alw26I GTCTC 2 cut(s) 17, 573
AlwI GGATC 2 cut(s) 343, 551
Aor13HI TCCGGA 1 cut(s) 378
AoxI GGCC 3 cut(s) 63, 85, 183
AspLEI GCGC 2 cut(s) 212, 487
AspS9I GGNCC 1 cut(s) 86
AsuC2I CCSGG 1 cut(s) 160
AsuHPI GGTGA 6 cut(s) 40, 70, 136, 244, 253, 439
BanII GRGCYC 1 cut(s) 141
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 2 cut(s) 141, 566
BbvCI CCTCAGC 1 cut(s) 230
BccI CCATC 4 cut(s) 224, 329, 350, 533
BceAI ACGGC 1 cut(s) 58
BcgI CGANNNNNNTGC 2 cut(s) 343, 377
BciT130I CCWGG 1 cut(s) 187
BclI TGATCA 1 cut(s) 489
BcnI CCSGG 1 cut(s) 160
BcoDI GTCTC 2 cut(s) 17, 573
BfoI RGCGCY 2 cut(s) 213, 488
BglI GCCNNNNNGGC 1 cut(s) 93
BisI GCNGC 3 cut(s) 94, 213, 483
BlsI GCNGC 3 cut(s) 95, 214, 484
Bme1390I CCNGG 2 cut(s) 160, 187
BmgBI CACGTC 1 cut(s) 238
BmgT120I GGNCC 1 cut(s) 86
BmiI GGNNCC 1 cut(s) 88
BmrFI CCNGG 2 cut(s) 160, 187
BmrI ACTGGG 1 cut(s) 321
BmsI GCATC 2 cut(s) 273, 381
BmuI ACTGGG 1 cut(s) 321
BpiI GAAGAC 1 cut(s) 11
Bpu10I CCTNAGC 1 cut(s) 230
BpuMI CCSGG 1 cut(s) 160
BsaHI GRCGYC 2 cut(s) 45, 258
BsaI GGTCTC 1 cut(s) 17
BsaJI CCNNGG 4 cut(s) 18, 27, 66, 91
BsaWI WCCGGW 2 cut(s) 378, 575
Bsc4I CCNNNNNNNGG 1 cut(s) 385
Bse118I RCCGGY 1 cut(s) 254
Bse1I ACTGG 1 cut(s) 327
Bse3DI GCAATG 1 cut(s) 567
BseAI TCCGGA 1 cut(s) 378
BseBI CCWGG 1 cut(s) 187
BseDI CCNNGG 4 cut(s) 18, 27, 66, 91
BseGI GGATG 5 cut(s) 33, 216, 321, 439, 544
BseLI CCNNNNNNNGG 1 cut(s) 385
BseMI GCAATG 1 cut(s) 567
BseMII CTCAG 1 cut(s) 244
BseNI ACTGG 1 cut(s) 327
BseRI GAGGAG 1 cut(s) 574
Bsh1236I CGCG 1 cut(s) 93
Bsh1285I CGRYCG 1 cut(s) 419
BshFI GGCC 3 cut(s) 65, 87, 185
BsiEI CGRYCG 1 cut(s) 419
BsiHKAI GWGCWC 2 cut(s) 141, 566
BsiSI CCGG 4 cut(s) 159, 255, 379, 576
BslI CCNNNNNNNGG 1 cut(s) 385
BsmAI GTCTC 2 cut(s) 17, 573
BsmBI CGTCTC 1 cut(s) 573
BsnI GGCC 3 cut(s) 65, 87, 185
Bso31I GGTCTC 1 cut(s) 17
Bsp1286I GDGCHC 2 cut(s) 141, 566
Bsp13I TCCGGA 1 cut(s) 378
Bsp143I GATC 5 cut(s) 309, 348, 408, 489, 543
Bsp19I CCATGG 1 cut(s) 27
BspACI CCGC 9 cut(s) 91, 93, 106, 213, 220, 225, 274, 352, 482
BspANI GGCC 3 cut(s) 65, 87, 185
BspCNI CTCAG 1 cut(s) 243
BspEI TCCGGA 1 cut(s) 378
BspFNI CGCG 1 cut(s) 93
BspLI GGNNCC 1 cut(s) 88
BspPI GGATC 2 cut(s) 343, 551
BspTNI GGTCTC 1 cut(s) 17
BsrDI GCAATG 1 cut(s) 567
BsrFI RCCGGY 1 cut(s) 254
BsrI ACTGG 1 cut(s) 327
BssAI RCCGGY 1 cut(s) 254
BssECI CCNNGG 4 cut(s) 18, 27, 66, 91
BssMI GATC 5 cut(s) 309, 348, 408, 489, 543
BssNI GRCGYC 2 cut(s) 45, 258
BssT1I CCWWGG 2 cut(s) 27, 66
Bst2UI CCWGG 1 cut(s) 187
Bst4CI ACNGT 2 cut(s) 52, 401
BstACI GRCGYC 2 cut(s) 45, 258
BstC8I GCNNGC 1 cut(s) 98
BstDEI CTNAG 1 cut(s) 230
BstDSI CCRYGG 2 cut(s) 27, 91
BstF5I GGATG 5 cut(s) 33, 216, 321, 439, 544
BstFNI CGCG 1 cut(s) 93
BstH2I RGCGCY 2 cut(s) 213, 488
BstHHI GCGC 2 cut(s) 212, 487
BstKTI GATC 5 cut(s) 312, 351, 411, 492, 546
BstMAI GTCTC 2 cut(s) 17, 573
BstMBI GATC 5 cut(s) 309, 348, 408, 489, 543
BstMCI CGRYCG 1 cut(s) 419
BstMWI GCNNNNNNNGC 3 cut(s) 93, 207, 561
BstNI CCWGG 1 cut(s) 187
BstSCI CCNGG 2 cut(s) 158, 185
BstUI CGCG 1 cut(s) 93
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 2 cut(s) 348, 543
BstYI RGATCY 2 cut(s) 348, 543
BsuRI GGCC 3 cut(s) 65, 87, 185
BtgI CCRYGG 2 cut(s) 27, 91
BtrI CACGTC 1 cut(s) 238
BtsCI GGATG 5 cut(s) 33, 216, 321, 439, 544
Cac8I GCNNGC 1 cut(s) 98
CfoI GCGC 2 cut(s) 212, 487
Cfr10I RCCGGY 1 cut(s) 254
Cfr13I GGNCC 1 cut(s) 86
Cfr42I CCGCGG 1 cut(s) 94
CseI GACGC 2 cut(s) 34, 247
CviAII CATG 2 cut(s) 28, 506
CviJI RGCY 9 cut(s) 65, 87, 100, 139, 172, 185, 234, 270, 439
CviKI_1 RGCY 9 cut(s) 65, 87, 100, 139, 172, 185, 234, 270, 439
DdeI CTNAG 1 cut(s) 230
DpnI GATC 5 cut(s) 311, 350, 410, 491, 545
DpnII GATC 5 cut(s) 309, 348, 408, 489, 543
DrdI GACNNNNNNGTC 1 cut(s) 13
DseDI GACNNNNNNGTC 1 cut(s) 13
Ecl136II GAGCTC 1 cut(s) 139
Eco130I CCWWGG 2 cut(s) 27, 66
Eco147I AGGCCT 1 cut(s) 185
Eco24I GRGCYC 1 cut(s) 141
Eco31I GGTCTC 1 cut(s) 17
Eco53kI GAGCTC 1 cut(s) 139
EcoICRI GAGCTC 1 cut(s) 139
EcoO109I RGGNCCY 1 cut(s) 86
EcoRII CCWGG 1 cut(s) 185
EcoT14I CCWWGG 2 cut(s) 27, 66
EcoT38I GRGCYC 1 cut(s) 141
ErhI CCWWGG 2 cut(s) 27, 66
Esp3I CGTCTC 1 cut(s) 573
FaeI CATG 2 cut(s) 31, 509
FaiI YATR 2 cut(s) 29, 507
FatI CATG 2 cut(s) 27, 505
FauI CCCGC 2 cut(s) 98, 113
FbaI TGATCA 1 cut(s) 489
FblI GTMKAC 2 cut(s) 240, 294
Fnu4HI GCNGC 3 cut(s) 94, 213, 483
FokI GGATG 5 cut(s) 20, 203, 308, 446, 551
FriOI GRGCYC 1 cut(s) 141
Fsp4HI GCNGC 3 cut(s) 94, 213, 483
GlaI GCGC 2 cut(s) 211, 486
GluI GCNGC 3 cut(s) 94, 213, 483
HaeII RGCGCY 2 cut(s) 213, 488
HaeIII GGCC 3 cut(s) 65, 87, 185
HapII CCGG 4 cut(s) 159, 255, 379, 576
HgaI GACGC 2 cut(s) 34, 247
HhaI GCGC 2 cut(s) 212, 487
Hin1I GRCGYC 2 cut(s) 45, 258
Hin1II CATG 2 cut(s) 31, 509
Hin6I GCGC 2 cut(s) 210, 485
HinP1I GCGC 2 cut(s) 210, 485
HincII GTYRAC 1 cut(s) 241
HindII GTYRAC 1 cut(s) 241
HinfI GANTC 4 cut(s) 10, 152, 288, 375
HpaII CCGG 4 cut(s) 159, 255, 379, 576
HphI GGTGA 6 cut(s) 40, 70, 136, 244, 253, 439
Hpy166II GTNNAC 3 cut(s) 156, 241, 295
Hpy188I TCNGA 4 cut(s) 22, 309, 421, 498
Hpy188III TCNNGA 3 cut(s) 346, 379, 536
Hpy8I GTNNAC 3 cut(s) 156, 241, 295
Hpy99I CGWCG 2 cut(s) 152, 242
HpyAV CCTTC 6 cut(s) 49, 55, 77, 376, 418, 424
HpyCH4III ACNGT 2 cut(s) 52, 401
HpyCH4IV ACGT 2 cut(s) 237, 549
HpyF10VI GCNNNNNNNGC 3 cut(s) 93, 207, 561
HpyF3I CTNAG 1 cut(s) 230
HpySE526I ACGT 2 cut(s) 237, 549
Hsp92I GRCGYC 2 cut(s) 45, 258
Hsp92II CATG 2 cut(s) 31, 509
HspAI GCGC 2 cut(s) 210, 485
Kpn2I TCCGGA 1 cut(s) 378
Ksp22I TGATCA 1 cut(s) 489
KspI CCGCGG 1 cut(s) 94
Kzo9I GATC 5 cut(s) 309, 348, 408, 489, 543
LmnI GCTCC 1 cut(s) 561
LweI GCATC 2 cut(s) 273, 381
MaeII ACGT 2 cut(s) 237, 549
MaeIII GTNAC 3 cut(s) 46, 259, 403
MalI GATC 5 cut(s) 311, 350, 410, 491, 545
MboI GATC 5 cut(s) 309, 348, 408, 489, 543
MboII GAAGA 4 cut(s) 16, 175, 178, 523
MflI RGATCY 2 cut(s) 348, 543
MhlI GDGCHC 2 cut(s) 141, 566
MlyI GAGTC 2 cut(s) 161, 384
MmeI TCCRAC 2 cut(s) 357, 399
MroI TCCGGA 1 cut(s) 378
MspA1I CMGCKG 1 cut(s) 93
MspI CCGG 4 cut(s) 159, 255, 379, 576
MspR9I CCNGG 2 cut(s) 160, 187
MvaI CCWGG 1 cut(s) 187
MvnI CGCG 1 cut(s) 93
MwoI GCNNNNNNNGC 3 cut(s) 93, 207, 561
NciI CCSGG 1 cut(s) 160
NcoI CCATGG 1 cut(s) 27
NdeII GATC 5 cut(s) 309, 348, 408, 489, 543
NlaIII CATG 2 cut(s) 31, 509
NlaIV GGNNCC 1 cut(s) 88
NmeAIII GCCGAG 1 cut(s) 342
NmuCI GTSAC 3 cut(s) 46, 259, 403
PceI AGGCCT 1 cut(s) 185
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PfeI GAWTC 2 cut(s) 10, 288
PflFI GACNNNGTC 1 cut(s) 44
PkrI GCNGC 3 cut(s) 95, 214, 484
PleI GAGTC 2 cut(s) 160, 383
PpsI GAGTC 2 cut(s) 160, 383
Psp124BI GAGCTC 1 cut(s) 141
Psp6I CCWGG 1 cut(s) 185
PspGI CCWGG 1 cut(s) 185
PspN4I GGNNCC 1 cut(s) 88
PspPI GGNCC 1 cut(s) 86
PsuI RGATCY 2 cut(s) 348, 543
PsyI GACNNNGTC 1 cut(s) 44
SacI GAGCTC 1 cut(s) 141
SacII CCGCGG 1 cut(s) 94
SalI GTCGAC 1 cut(s) 239
SatI GCNGC 3 cut(s) 94, 213, 483
Sau3AI GATC 5 cut(s) 309, 348, 408, 489, 543
Sau96I GGNCC 1 cut(s) 86
SchI GAGTC 2 cut(s) 161, 384
ScrFI CCNGG 2 cut(s) 160, 187
SduI GDGCHC 2 cut(s) 141, 566
SfaNI GCATC 2 cut(s) 273, 381
Sfr303I CCGCGG 1 cut(s) 94
SgrAI CRCCGGYG 1 cut(s) 254
SgrBI CCGCGG 1 cut(s) 94
SseBI AGGCCT 1 cut(s) 185
SsiI CCGC 9 cut(s) 91, 93, 106, 213, 220, 225, 274, 352, 482
SstI GAGCTC 1 cut(s) 141
StuI AGGCCT 1 cut(s) 185
StyD4I CCNGG 2 cut(s) 158, 185
StyI CCWWGG 2 cut(s) 27, 66
TaaI ACNGT 2 cut(s) 52, 401
TaiI ACGT 2 cut(s) 240, 552
TaqI TCGA 8 cut(s) 75, 81, 129, 135, 180, 240, 345, 473
TaqII GACCGA 1 cut(s) 405
TauI GCSGC 3 cut(s) 96, 215, 485
TfiI GAWTC 2 cut(s) 10, 288
TseFI GTSAC 3 cut(s) 46, 259, 403
Tsp45I GTSAC 3 cut(s) 46, 259, 403
TspDTI ATGAA 1 cut(s) 17
Tth111I GACNNNGTC 1 cut(s) 44
XmiI GTMKAC 2 cut(s) 240, 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.