Rh7AG091500

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0001380
Physical Location & Seq
Forward (+)
7004753 .. 7005340
588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG091500.1

Sequence Viewer

Length: 588 bp
ATGAAGACGATTCTGGCCTCGGAGAGCATGGACATCCCGGACGGCGTCACCATCAAGGTGAAGGCCAAGGTCATTGAGGTCGAAGGTCCCCGCGGGAAGCTCACCCGAAACTTCAAGCACCTCAACCTCGACTTCGAGCTCGTCACCGAGGAGTCGACGGGGAAAAAGAAGCTCAAGATCGAGGCCTGGTTCGGCACCCGCAAGACCAGCGCCGCCATCCGCACCGCGCTCAGCCACGTCAGCAACCTCATCATCGGCGTCACCAAGGGCTACCGCTACAAGATGAGGTTCGTGTACGCTCACTTTCCGATCAACGCCTCCATCCCCAGCTCCAGCAACGCCATCGAGATCCGCAACTTCCTGGGAGAGAAGAAAGTCAGGAAGGTCCAGATGCTGGAAGGCGTGACGATCTCGCGATCGGAGAAGGTGAAGGATGAGCTGGTTTTGGACGGGAACGACATCGAGTTGGTTTCGAGGTCGGCGGCGCTGATCAACCAGAAGTGTCATGTGAAGAACAAGGACATCAGGAAGTTTCTGGATGGGATCTACGTCAGCGAGAGGAGCACCATTACCACCGGAGACGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L6

Protein Analysis

195

Amino Acids

21.9

Weight (kDa)

9.66

Isoelectric Point (pI)

29.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L6 PF00347 12 - 90 2.5e-10 Ribosomal protein L6
Ribosomal_L6 PF00347 115 - 181 5e-13 Ribosomal protein L6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 194
AccB7I CCANNNNNTGG 1 cut(s) 394
AccI GTMKAC 1 cut(s) 155
AccII CGCG 3 cut(s) 93, 227, 415
AciI CCGC 9 cut(s) 91, 93, 199, 213, 220, 225, 274, 352, 482
AclWI GGATC 2 cut(s) 343, 551
AcyI GRCGYC 2 cut(s) 45, 258
AfaI GTAC 1 cut(s) 296
AfiI CCNNNNNNNGG 1 cut(s) 394
AgsI TTSAA 1 cut(s) 115
AjiI CACGTC 1 cut(s) 238
AjnI CCWGG 2 cut(s) 185, 360
AluBI AGCT 5 cut(s) 100, 139, 172, 330, 439
AluI AGCT 5 cut(s) 100, 139, 172, 330, 439
Alw21I GWGCWC 2 cut(s) 141, 566
Alw26I GTCTC 1 cut(s) 573
AlwI GGATC 2 cut(s) 343, 551
AlwNI CAGNNNCTG 1 cut(s) 394
AoxI GGCC 3 cut(s) 15, 63, 183
AspLEI GCGC 3 cut(s) 212, 229, 487
AspS9I GGNCC 2 cut(s) 86, 385
AsuC2I CCSGG 1 cut(s) 38
AsuHPI GGTGA 6 cut(s) 40, 70, 94, 136, 253, 439
AvaII GGWCC 2 cut(s) 86, 385
BanI GGYRCC 1 cut(s) 194
BanII GRGCYC 1 cut(s) 141
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 2 cut(s) 141, 566
BccI CCATC 5 cut(s) 59, 224, 329, 350, 533
BceAI ACGGC 1 cut(s) 58
BcgI CGANNNNNNTGC 2 cut(s) 325, 359
BciT130I CCWGG 2 cut(s) 187, 362
BclI TGATCA 1 cut(s) 489
BcnI CCSGG 1 cut(s) 38
BcoDI GTCTC 1 cut(s) 573
BfoI RGCGCY 2 cut(s) 213, 488
BisI GCNGC 2 cut(s) 213, 483
BlpI GCTNAGC 1 cut(s) 230
BlsI GCNGC 2 cut(s) 214, 484
Bme1390I CCNGG 3 cut(s) 38, 187, 362
Bme18I GGWCC 2 cut(s) 86, 385
BmgBI CACGTC 1 cut(s) 238
BmgT120I GGNCC 2 cut(s) 86, 385
BmiI GGNNCC 2 cut(s) 88, 196
BmrFI CCNGG 3 cut(s) 38, 187, 362
BmsI GCATC 1 cut(s) 381
BpiI GAAGAC 1 cut(s) 11
BpmI CTGGAG 1 cut(s) 316
Bpu1102I GCTNAGC 1 cut(s) 230
BpuEI CTTGAG 1 cut(s) 158
BpuMI CCSGG 1 cut(s) 38
BsaHI GRCGYC 2 cut(s) 45, 258
BsaJI CCNNGG 6 cut(s) 18, 66, 91, 147, 264, 361
BsaWI WCCGGW 1 cut(s) 575
Bsc4I CCNNNNNNNGG 1 cut(s) 394
BseBI CCWGG 2 cut(s) 187, 362
BseDI CCNNGG 6 cut(s) 18, 66, 91, 147, 264, 361
BseGI GGATG 5 cut(s) 33, 216, 321, 439, 544
BseLI CCNNNNNNNGG 1 cut(s) 394
BseMII CTCAG 1 cut(s) 244
BseRI GAGGAG 2 cut(s) 164, 574
BseYI CCCAGC 1 cut(s) 326
Bsh1236I CGCG 3 cut(s) 93, 227, 415
Bsh1285I CGRYCG 1 cut(s) 419
BshFI GGCC 3 cut(s) 17, 65, 185
BshNI GGYRCC 1 cut(s) 194
BsiEI CGRYCG 1 cut(s) 419
BsiHKAI GWGCWC 2 cut(s) 141, 566
BsiSI CCGG 2 cut(s) 38, 576
BslFI GGGAC 1 cut(s) 72
BslI CCNNNNNNNGG 1 cut(s) 394
BsmAI GTCTC 1 cut(s) 573
BsmBI CGTCTC 1 cut(s) 573
BsmFI GGGAC 1 cut(s) 72
BsnI GGCC 3 cut(s) 17, 65, 185
Bsp1286I GDGCHC 2 cut(s) 141, 566
Bsp143I GATC 7 cut(s) 177, 309, 348, 408, 416, 489, 543
Bsp1720I GCTNAGC 1 cut(s) 230
Bsp68I TCGCGA 1 cut(s) 415
BspACI CCGC 9 cut(s) 91, 93, 199, 213, 220, 225, 274, 352, 482
BspANI GGCC 3 cut(s) 17, 65, 185
BspCNI CTCAG 1 cut(s) 243
BspFNI CGCG 3 cut(s) 93, 227, 415
BspLI GGNNCC 2 cut(s) 88, 196
BspPI GGATC 2 cut(s) 343, 551
BspT107I GGYRCC 1 cut(s) 194
BssECI CCNNGG 6 cut(s) 18, 66, 91, 147, 264, 361
BssMI GATC 7 cut(s) 177, 309, 348, 408, 416, 489, 543
BssNI GRCGYC 2 cut(s) 45, 258
BssT1I CCWWGG 2 cut(s) 66, 264
Bst2UI CCWGG 2 cut(s) 187, 362
BstACI GRCGYC 2 cut(s) 45, 258
BstDEI CTNAG 1 cut(s) 230
BstDSI CCRYGG 1 cut(s) 91
BstF5I GGATG 5 cut(s) 33, 216, 321, 439, 544
BstFNI CGCG 3 cut(s) 93, 227, 415
BstH2I RGCGCY 2 cut(s) 213, 488
BstHHI GCGC 3 cut(s) 212, 229, 487
BstKTI GATC 7 cut(s) 180, 312, 351, 411, 419, 492, 546
BstMAI GTCTC 1 cut(s) 573
BstMBI GATC 7 cut(s) 177, 309, 348, 408, 416, 489, 543
BstMCI CGRYCG 1 cut(s) 419
BstMWI GCNNNNNNNGC 3 cut(s) 207, 240, 561
BstNI CCWGG 2 cut(s) 187, 362
BstSCI CCNGG 3 cut(s) 36, 185, 360
BstUI CGCG 3 cut(s) 93, 227, 415
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 2 cut(s) 348, 543
BstYI RGATCY 2 cut(s) 348, 543
BsuRI GGCC 3 cut(s) 17, 65, 185
BtgI CCRYGG 1 cut(s) 91
BtrI CACGTC 1 cut(s) 238
BtsCI GGATG 5 cut(s) 33, 216, 321, 439, 544
BtuMI TCGCGA 1 cut(s) 415
CaiI CAGNNNCTG 1 cut(s) 394
CfoI GCGC 3 cut(s) 212, 229, 487
Cfr13I GGNCC 2 cut(s) 86, 385
Cfr42I CCGCGG 1 cut(s) 94
CseI GACGC 2 cut(s) 34, 247
Csp6I GTAC 1 cut(s) 295
CviAII CATG 2 cut(s) 28, 506
CviQI GTAC 1 cut(s) 295
DdeI CTNAG 1 cut(s) 230
DpnI GATC 7 cut(s) 179, 311, 350, 410, 418, 491, 545
DpnII GATC 7 cut(s) 177, 309, 348, 408, 416, 489, 543
Ecl136II GAGCTC 1 cut(s) 139
Eco130I CCWWGG 2 cut(s) 66, 264
Eco147I AGGCCT 1 cut(s) 185
Eco24I GRGCYC 1 cut(s) 141
Eco47I GGWCC 2 cut(s) 86, 385
Eco53kI GAGCTC 1 cut(s) 139
EcoICRI GAGCTC 1 cut(s) 139
EcoO109I RGGNCCY 1 cut(s) 86
EcoRII CCWGG 2 cut(s) 185, 360
EcoT14I CCWWGG 2 cut(s) 66, 264
EcoT38I GRGCYC 1 cut(s) 141
ErhI CCWWGG 2 cut(s) 66, 264
Esp3I CGTCTC 1 cut(s) 573
FaeI CATG 2 cut(s) 31, 509
FaiI YATR 2 cut(s) 29, 507
FaqI GGGAC 1 cut(s) 72
FatI CATG 2 cut(s) 27, 505
FauI CCCGC 3 cut(s) 86, 98, 206
FbaI TGATCA 1 cut(s) 489
FblI GTMKAC 1 cut(s) 155
Fnu4HI GCNGC 2 cut(s) 213, 483
FokI GGATG 5 cut(s) 20, 203, 308, 446, 551
FriOI GRGCYC 1 cut(s) 141
Fsp4HI GCNGC 2 cut(s) 213, 483
GlaI GCGC 3 cut(s) 211, 228, 486
GluI GCNGC 2 cut(s) 213, 483
GsaI CCCAGC 1 cut(s) 330
GsuI CTGGAG 1 cut(s) 316
HaeII RGCGCY 2 cut(s) 213, 488
HaeIII GGCC 3 cut(s) 17, 65, 185
HapII CCGG 2 cut(s) 38, 576
HgaI GACGC 2 cut(s) 34, 247
HhaI GCGC 3 cut(s) 212, 229, 487
Hin1I GRCGYC 2 cut(s) 45, 258
Hin1II CATG 2 cut(s) 31, 509
Hin6I GCGC 3 cut(s) 210, 227, 485
HinP1I GCGC 3 cut(s) 210, 227, 485
HincII GTYRAC 1 cut(s) 156
HindII GTYRAC 1 cut(s) 156
HinfI GANTC 2 cut(s) 10, 152
HpaII CCGG 2 cut(s) 38, 576
HphI GGTGA 6 cut(s) 40, 70, 94, 136, 253, 439
Hpy166II GTNNAC 2 cut(s) 156, 295
Hpy188I TCNGA 3 cut(s) 22, 309, 421
Hpy188III TCNNGA 7 cut(s) 175, 346, 379, 388, 414, 526, 536
Hpy8I GTNNAC 2 cut(s) 156, 295
Hpy99I CGWCG 1 cut(s) 160
HpyAV CCTTC 6 cut(s) 55, 77, 376, 392, 418, 424
HpyCH4IV ACGT 2 cut(s) 237, 549
HpyF10VI GCNNNNNNNGC 3 cut(s) 207, 240, 561
HpyF3I CTNAG 1 cut(s) 230
HpySE526I ACGT 2 cut(s) 237, 549
Hsp92I GRCGYC 2 cut(s) 45, 258
Hsp92II CATG 2 cut(s) 31, 509
HspAI GCGC 3 cut(s) 210, 227, 485
Ksp22I TGATCA 1 cut(s) 489
KspI CCGCGG 1 cut(s) 94
Kzo9I GATC 7 cut(s) 177, 309, 348, 408, 416, 489, 543
LmnI GCTCC 2 cut(s) 335, 561
LweI GCATC 1 cut(s) 381
MaeII ACGT 2 cut(s) 237, 549
MaeIII GTNAC 4 cut(s) 46, 142, 259, 403
MalI GATC 7 cut(s) 179, 311, 350, 410, 418, 491, 545
MboI GATC 7 cut(s) 177, 309, 348, 408, 416, 489, 543
MboII GAAGA 3 cut(s) 16, 382, 523
MflI RGATCY 2 cut(s) 348, 543
MhlI GDGCHC 2 cut(s) 141, 566
MlyI GAGTC 1 cut(s) 161
MslI CAYNNNNRTG 1 cut(s) 56
MspA1I CMGCKG 1 cut(s) 93
MspI CCGG 2 cut(s) 38, 576
MspR9I CCNGG 3 cut(s) 38, 187, 362
MvaI CCWGG 2 cut(s) 187, 362
MvnI CGCG 3 cut(s) 93, 227, 415
MwoI GCNNNNNNNGC 3 cut(s) 207, 240, 561
NciI CCSGG 1 cut(s) 38
NdeII GATC 7 cut(s) 177, 309, 348, 408, 416, 489, 543
NlaIII CATG 2 cut(s) 31, 509
NlaIV GGNNCC 2 cut(s) 88, 196
NmuCI GTSAC 4 cut(s) 46, 142, 259, 403
NruI TCGCGA 1 cut(s) 415
PceI AGGCCT 1 cut(s) 185
PfeI GAWTC 1 cut(s) 10
PflFI GACNNNGTC 1 cut(s) 44
PflMI CCANNNNNTGG 1 cut(s) 394
PfoI TCCNGGA 1 cut(s) 36
PkrI GCNGC 2 cut(s) 214, 484
Ple19I CGATCG 1 cut(s) 419
PleI GAGTC 1 cut(s) 160
PpsI GAGTC 1 cut(s) 160
PpuMI RGGWCCY 1 cut(s) 86
Psp124BI GAGCTC 1 cut(s) 141
Psp5II RGGWCCY 1 cut(s) 86
Psp6I CCWGG 2 cut(s) 185, 360
PspFI CCCAGC 1 cut(s) 326
PspGI CCWGG 2 cut(s) 185, 360
PspN4I GGNNCC 2 cut(s) 88, 196
PspPI GGNCC 2 cut(s) 86, 385
PspPPI RGGWCCY 1 cut(s) 86
PstNI CAGNNNCTG 1 cut(s) 394
PsuI RGATCY 2 cut(s) 348, 543
PsyI GACNNNGTC 1 cut(s) 44
PvuI CGATCG 1 cut(s) 419
RruI TCGCGA 1 cut(s) 415
RsaI GTAC 1 cut(s) 296
RsaNI GTAC 1 cut(s) 295
RseI CAYNNNNRTG 1 cut(s) 56
SacI GAGCTC 1 cut(s) 141
SacII CCGCGG 1 cut(s) 94
SalI GTCGAC 1 cut(s) 154
SatI GCNGC 2 cut(s) 213, 483
Sau3AI GATC 7 cut(s) 177, 309, 348, 408, 416, 489, 543
Sau96I GGNCC 2 cut(s) 86, 385
SchI GAGTC 1 cut(s) 161
ScrFI CCNGG 3 cut(s) 38, 187, 362
SduI GDGCHC 2 cut(s) 141, 566
SfaNI GCATC 1 cut(s) 381
Sfr303I CCGCGG 1 cut(s) 94
SgrBI CCGCGG 1 cut(s) 94
SinI GGWCC 2 cut(s) 86, 385
SmiMI CAYNNNNRTG 1 cut(s) 56
SmlI CTYRAG 1 cut(s) 173
SmoI CTYRAG 1 cut(s) 173
SseBI AGGCCT 1 cut(s) 185
SsiI CCGC 9 cut(s) 91, 93, 199, 213, 220, 225, 274, 352, 482
SstI GAGCTC 1 cut(s) 141
StuI AGGCCT 1 cut(s) 185
StyD4I CCNGG 3 cut(s) 36, 185, 360
StyI CCWWGG 2 cut(s) 66, 264
TaiI ACGT 2 cut(s) 240, 552
TaqI TCGA 8 cut(s) 81, 129, 135, 155, 180, 345, 462, 473
TauI GCSGC 2 cut(s) 215, 485
TfiI GAWTC 1 cut(s) 10
TseFI GTSAC 4 cut(s) 46, 142, 259, 403
Tsp45I GTSAC 4 cut(s) 46, 142, 259, 403
TspDTI ATGAA 1 cut(s) 17
Tth111I GACNNNGTC 1 cut(s) 44
Van91I CCANNNNNTGG 1 cut(s) 394
VpaK11BI GGWCC 2 cut(s) 86, 385
XmiI GTMKAC 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.