RchiOBHm_Chr3g0462551

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0001380
Physical Location & Seq
Forward (+)
10223628 .. 10224543
916 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42890

Sequence Viewer

Length: 726 bp
ATGAAGACGATTCTGGCCTCGGAGAGCATGGACATCCCGGACGGCGTCACCATCAAGGTGAAGGCCAAGGTCATCGAGGTCGAAGGTCCCCGCGGGAAGCTCACCCGAAACTTCAAGCACCTCAACCTCGACTTCGAGCTCGTCACCGACGAGTCGACGGGGAAAAAGAAGCTGAAGATCGAGGCCTGGTTCGGCACCCGCAAGACCAGAGCCGCCATCCGCACCGCGCTCAGCCACGTCAGCAACCTCATCATCGGCGTCACCAAGGGCTACCGCTACAAGATGAGGTTCGTCTACGCTCACTTTCCGATCAACGCCTCCATCCCCAGCTCCAGCAACGCCATCGAGATCCGCAACTTCCTGGGAGTGAAGAAAGTCAGAAAGGTCCAGATGCTCGAAGGCGTGACGATCTCGCGATCGGAGAAGGTGAAGGATGAGATGGTTTTGGACGGGAACGACATCGAGTTGGTTTCGAGGTCGGCGGCGCTGATCAACCAGAAGTGTCATGTGAAGAACAAGGACATCAGGAAGTTTCTGGATGGGATCTACGTCAGCGAGAGGAGCACCATTACCACCGGAGACGAAGGAACAAGAATCGATCGATTAGTCCAATATCGTGAGATTGTGGAGATTTTGCTTTTGATATGGGTTTGTGTTATCAAAGATTTCGTCAGATTTGTGGAACTGCAGCTTGGTTTTGTTAAATTAGAGCTTTTCAGTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L6

Protein Analysis

241

Amino Acids

27.52

Weight (kDa)

9.67

Isoelectric Point (pI)

24.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L6 PF00347 12 - 90 1.5e-10 Ribosomal protein L6
Ribosomal_L6 PF00347 115 - 181 1e-11 Ribosomal protein L6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 194
AccI GTMKAC 2 cut(s) 155, 294
AccII CGCG 3 cut(s) 93, 227, 415
AciI CCGC 9 cut(s) 91, 93, 199, 213, 220, 225, 274, 352, 482
AclWI GGATC 2 cut(s) 343, 551
AcuI CTGAAG 1 cut(s) 194
AcyI GRCGYC 2 cut(s) 45, 258
AgsI TTSAA 1 cut(s) 115
AjiI CACGTC 1 cut(s) 238
AjnI CCWGG 2 cut(s) 185, 360
AjuI GAANNNNNNNTTGG 2 cut(s) 675, 707
AluBI AGCT 6 cut(s) 100, 139, 172, 330, 691, 712
AluI AGCT 6 cut(s) 100, 139, 172, 330, 691, 712
Alw21I GWGCWC 2 cut(s) 141, 566
Alw26I GTCTC 1 cut(s) 573
AlwI GGATC 2 cut(s) 343, 551
AoxI GGCC 3 cut(s) 15, 63, 183
ApeKI GCWGC 1 cut(s) 688
ArsI GACNNNNNNTTYG 2 cut(s) 654, 686
AspLEI GCGC 2 cut(s) 229, 487
AspS9I GGNCC 2 cut(s) 86, 385
AsuC2I CCSGG 1 cut(s) 38
AsuHPI GGTGA 6 cut(s) 40, 70, 94, 136, 253, 439
AvaII GGWCC 2 cut(s) 86, 385
BanI GGYRCC 1 cut(s) 194
BanII GRGCYC 1 cut(s) 141
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 2 cut(s) 141, 566
BbvI GCAGC 1 cut(s) 700
BccI CCATC 6 cut(s) 59, 224, 329, 350, 433, 533
BceAI ACGGC 1 cut(s) 58
BcgI CGANNNNNNTGC 2 cut(s) 325, 359
BciT130I CCWGG 2 cut(s) 187, 362
BclI TGATCA 1 cut(s) 489
BcnI CCSGG 1 cut(s) 38
BcoDI GTCTC 1 cut(s) 573
BfmI CTRYAG 1 cut(s) 686
BfoI RGCGCY 1 cut(s) 488
BisI GCNGC 3 cut(s) 213, 483, 689
BlpI GCTNAGC 1 cut(s) 230
BlsI GCNGC 3 cut(s) 214, 484, 690
Bme1390I CCNGG 3 cut(s) 38, 187, 362
Bme18I GGWCC 2 cut(s) 86, 385
BmgBI CACGTC 1 cut(s) 238
BmgT120I GGNCC 2 cut(s) 86, 385
BmiI GGNNCC 2 cut(s) 88, 196
BmrFI CCNGG 3 cut(s) 38, 187, 362
BmsI GCATC 1 cut(s) 381
BpiI GAAGAC 1 cut(s) 11
BpmI CTGGAG 1 cut(s) 316
Bpu1102I GCTNAGC 1 cut(s) 230
BpuMI CCSGG 1 cut(s) 38
Bsa29I ATCGAT 2 cut(s) 597, 601
BsaHI GRCGYC 2 cut(s) 45, 258
BsaJI CCNNGG 5 cut(s) 18, 66, 91, 264, 361
BsaWI WCCGGW 1 cut(s) 575
BseBI CCWGG 2 cut(s) 187, 362
BseCI ATCGAT 2 cut(s) 597, 601
BseDI CCNNGG 5 cut(s) 18, 66, 91, 264, 361
BseGI GGATG 5 cut(s) 33, 216, 321, 439, 544
BseMII CTCAG 1 cut(s) 244
BseRI GAGGAG 1 cut(s) 574
BseXI GCAGC 1 cut(s) 700
BseYI CCCAGC 1 cut(s) 326
Bsh1236I CGCG 3 cut(s) 93, 227, 415
Bsh1285I CGRYCG 2 cut(s) 419, 601
BshFI GGCC 3 cut(s) 17, 65, 185
BshNI GGYRCC 1 cut(s) 194
BshVI ATCGAT 2 cut(s) 597, 601
BsiEI CGRYCG 2 cut(s) 419, 601
BsiHKAI GWGCWC 2 cut(s) 141, 566
BsiSI CCGG 2 cut(s) 38, 576
BslFI GGGAC 1 cut(s) 72
BsmAI GTCTC 1 cut(s) 573
BsmBI CGTCTC 1 cut(s) 573
BsmFI GGGAC 1 cut(s) 72
BsnI GGCC 3 cut(s) 17, 65, 185
Bsp1286I GDGCHC 2 cut(s) 141, 566
Bsp143I GATC 8 cut(s) 177, 309, 348, 408, 416, 489, 543, 598
Bsp1720I GCTNAGC 1 cut(s) 230
Bsp68I TCGCGA 1 cut(s) 415
BspACI CCGC 9 cut(s) 91, 93, 199, 213, 220, 225, 274, 352, 482
BspANI GGCC 3 cut(s) 17, 65, 185
BspCNI CTCAG 1 cut(s) 243
BspDI ATCGAT 2 cut(s) 597, 601
BspFNI CGCG 3 cut(s) 93, 227, 415
BspLI GGNNCC 2 cut(s) 88, 196
BspMAI CTGCAG 1 cut(s) 690
BspPI GGATC 2 cut(s) 343, 551
BspT107I GGYRCC 1 cut(s) 194
BssECI CCNNGG 5 cut(s) 18, 66, 91, 264, 361
BssMI GATC 8 cut(s) 177, 309, 348, 408, 416, 489, 543, 598
BssNI GRCGYC 2 cut(s) 45, 258
BssT1I CCWWGG 2 cut(s) 66, 264
Bst2UI CCWGG 2 cut(s) 187, 362
BstACI GRCGYC 2 cut(s) 45, 258
BstDEI CTNAG 1 cut(s) 230
BstDSI CCRYGG 1 cut(s) 91
BstF5I GGATG 5 cut(s) 33, 216, 321, 439, 544
BstFNI CGCG 3 cut(s) 93, 227, 415
BstH2I RGCGCY 1 cut(s) 488
BstHHI GCGC 2 cut(s) 229, 487
BstKTI GATC 8 cut(s) 180, 312, 351, 411, 419, 492, 546, 601
BstMAI GTCTC 1 cut(s) 573
BstMBI GATC 8 cut(s) 177, 309, 348, 408, 416, 489, 543, 598
BstMCI CGRYCG 2 cut(s) 419, 601
BstMWI GCNNNNNNNGC 2 cut(s) 240, 561
BstNI CCWGG 2 cut(s) 187, 362
BstSCI CCNGG 3 cut(s) 36, 185, 360
BstSFI CTRYAG 1 cut(s) 686
BstUI CGCG 3 cut(s) 93, 227, 415
BstV1I GCAGC 1 cut(s) 700
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 2 cut(s) 348, 543
BstYI RGATCY 2 cut(s) 348, 543
Bsu15I ATCGAT 2 cut(s) 597, 601
BsuRI GGCC 3 cut(s) 17, 65, 185
BsuTUI ATCGAT 2 cut(s) 597, 601
BtgI CCRYGG 1 cut(s) 91
BtrI CACGTC 1 cut(s) 238
BtsCI GGATG 5 cut(s) 33, 216, 321, 439, 544
BtuMI TCGCGA 1 cut(s) 415
CfoI GCGC 2 cut(s) 229, 487
Cfr13I GGNCC 2 cut(s) 86, 385
Cfr42I CCGCGG 1 cut(s) 94
ClaI ATCGAT 2 cut(s) 597, 601
CseI GACGC 2 cut(s) 34, 247
CviAII CATG 2 cut(s) 28, 506
DdeI CTNAG 1 cut(s) 230
DpnI GATC 8 cut(s) 179, 311, 350, 410, 418, 491, 545, 600
DpnII GATC 8 cut(s) 177, 309, 348, 408, 416, 489, 543, 598
Ecl136II GAGCTC 1 cut(s) 139
Eco130I CCWWGG 2 cut(s) 66, 264
Eco147I AGGCCT 1 cut(s) 185
Eco24I GRGCYC 1 cut(s) 141
Eco47I GGWCC 2 cut(s) 86, 385
Eco53kI GAGCTC 1 cut(s) 139
Eco57I CTGAAG 1 cut(s) 194
EcoICRI GAGCTC 1 cut(s) 139
EcoO109I RGGNCCY 1 cut(s) 86
EcoRII CCWGG 2 cut(s) 185, 360
EcoT14I CCWWGG 2 cut(s) 66, 264
EcoT38I GRGCYC 1 cut(s) 141
ErhI CCWWGG 2 cut(s) 66, 264
Esp3I CGTCTC 1 cut(s) 573
FaeI CATG 2 cut(s) 31, 509
FaiI YATR 3 cut(s) 29, 507, 646
FaqI GGGAC 1 cut(s) 72
FatI CATG 2 cut(s) 27, 505
FauI CCCGC 3 cut(s) 86, 98, 206
FbaI TGATCA 1 cut(s) 489
FblI GTMKAC 2 cut(s) 155, 294
Fnu4HI GCNGC 3 cut(s) 213, 483, 689
FokI GGATG 5 cut(s) 20, 203, 308, 446, 551
FriOI GRGCYC 1 cut(s) 141
Fsp4HI GCNGC 3 cut(s) 213, 483, 689
GlaI GCGC 2 cut(s) 228, 486
GluI GCNGC 3 cut(s) 213, 483, 689
GsaI CCCAGC 1 cut(s) 330
GsuI CTGGAG 1 cut(s) 316
HaeII RGCGCY 1 cut(s) 488
HaeIII GGCC 3 cut(s) 17, 65, 185
HapII CCGG 2 cut(s) 38, 576
HgaI GACGC 2 cut(s) 34, 247
HhaI GCGC 2 cut(s) 229, 487
Hin1I GRCGYC 2 cut(s) 45, 258
Hin1II CATG 2 cut(s) 31, 509
Hin6I GCGC 2 cut(s) 227, 485
HinP1I GCGC 2 cut(s) 227, 485
HincII GTYRAC 1 cut(s) 156
HindII GTYRAC 1 cut(s) 156
HinfI GANTC 3 cut(s) 10, 152, 594
HpaII CCGG 2 cut(s) 38, 576
HphI GGTGA 6 cut(s) 40, 70, 94, 136, 253, 439
Hpy166II GTNNAC 2 cut(s) 156, 295
Hpy188I TCNGA 5 cut(s) 22, 309, 380, 421, 674
Hpy188III TCNNGA 6 cut(s) 346, 388, 414, 526, 536, 617
Hpy8I GTNNAC 2 cut(s) 156, 295
Hpy99I CGWCG 2 cut(s) 152, 160
HpyAV CCTTC 6 cut(s) 55, 77, 392, 418, 424, 578
HpyCH4IV ACGT 2 cut(s) 237, 549
HpyCH4V TGCA 1 cut(s) 688
HpyF10VI GCNNNNNNNGC 2 cut(s) 240, 561
HpyF3I CTNAG 1 cut(s) 230
HpySE526I ACGT 2 cut(s) 237, 549
Hsp92I GRCGYC 2 cut(s) 45, 258
Hsp92II CATG 2 cut(s) 31, 509
HspAI GCGC 2 cut(s) 227, 485
Ksp22I TGATCA 1 cut(s) 489
KspI CCGCGG 1 cut(s) 94
Kzo9I GATC 8 cut(s) 177, 309, 348, 408, 416, 489, 543, 598
LmnI GCTCC 2 cut(s) 335, 561
Lsp1109I GCAGC 1 cut(s) 700
LweI GCATC 1 cut(s) 381
MaeII ACGT 2 cut(s) 237, 549
MaeIII GTNAC 4 cut(s) 46, 142, 259, 403
MalI GATC 8 cut(s) 179, 311, 350, 410, 418, 491, 545, 600
MboI GATC 8 cut(s) 177, 309, 348, 408, 416, 489, 543, 598
MboII GAAGA 4 cut(s) 16, 187, 382, 523
MflI RGATCY 2 cut(s) 348, 543
MhlI GDGCHC 2 cut(s) 141, 566
MluCI AATT 1 cut(s) 704
MlyI GAGTC 1 cut(s) 161
MseI TTAA 1 cut(s) 702
MslI CAYNNNNRTG 1 cut(s) 56
MspA1I CMGCKG 1 cut(s) 93
MspI CCGG 2 cut(s) 38, 576
MspR9I CCNGG 3 cut(s) 38, 187, 362
MvaI CCWGG 2 cut(s) 187, 362
MvnI CGCG 3 cut(s) 93, 227, 415
MwoI GCNNNNNNNGC 2 cut(s) 240, 561
NciI CCSGG 1 cut(s) 38
NdeII GATC 8 cut(s) 177, 309, 348, 408, 416, 489, 543, 598
NlaIII CATG 2 cut(s) 31, 509
NlaIV GGNNCC 2 cut(s) 88, 196
NmuCI GTSAC 4 cut(s) 46, 142, 259, 403
NruI TCGCGA 1 cut(s) 415
PceI AGGCCT 1 cut(s) 185
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PfeI GAWTC 2 cut(s) 10, 594
PflFI GACNNNGTC 1 cut(s) 44
PfoI TCCNGGA 1 cut(s) 36
PkrI GCNGC 3 cut(s) 214, 484, 690
Ple19I CGATCG 2 cut(s) 419, 601
PleI GAGTC 1 cut(s) 160
PpsI GAGTC 1 cut(s) 160
PpuMI RGGWCCY 1 cut(s) 86
Psp124BI GAGCTC 1 cut(s) 141
Psp5II RGGWCCY 1 cut(s) 86
Psp6I CCWGG 2 cut(s) 185, 360
PspFI CCCAGC 1 cut(s) 326
PspGI CCWGG 2 cut(s) 185, 360
PspN4I GGNNCC 2 cut(s) 88, 196
PspPI GGNCC 2 cut(s) 86, 385
PspPPI RGGWCCY 1 cut(s) 86
PstI CTGCAG 1 cut(s) 690
PsuI RGATCY 2 cut(s) 348, 543
PsyI GACNNNGTC 1 cut(s) 44
PvuI CGATCG 2 cut(s) 419, 601
RruI TCGCGA 1 cut(s) 415
RseI CAYNNNNRTG 1 cut(s) 56
SacI GAGCTC 1 cut(s) 141
SacII CCGCGG 1 cut(s) 94
SalI GTCGAC 1 cut(s) 154
SaqAI TTAA 1 cut(s) 702
SatI GCNGC 3 cut(s) 213, 483, 689
Sau3AI GATC 8 cut(s) 177, 309, 348, 408, 416, 489, 543, 598
Sau96I GGNCC 2 cut(s) 86, 385
SchI GAGTC 1 cut(s) 161
ScrFI CCNGG 3 cut(s) 38, 187, 362
SduI GDGCHC 2 cut(s) 141, 566
SfaNI GCATC 1 cut(s) 381
SfcI CTRYAG 1 cut(s) 686
Sfr303I CCGCGG 1 cut(s) 94
SgrBI CCGCGG 1 cut(s) 94
SinI GGWCC 2 cut(s) 86, 385
SmiMI CAYNNNNRTG 1 cut(s) 56
Sse9I AATT 1 cut(s) 704
SseBI AGGCCT 1 cut(s) 185
SsiI CCGC 9 cut(s) 91, 93, 199, 213, 220, 225, 274, 352, 482
SstI GAGCTC 1 cut(s) 141
StuI AGGCCT 1 cut(s) 185
StyD4I CCNGG 3 cut(s) 36, 185, 360
StyI CCWWGG 2 cut(s) 66, 264
TaiI ACGT 2 cut(s) 240, 552
TasI AATT 1 cut(s) 704
TauI GCSGC 2 cut(s) 215, 485
TfiI GAWTC 2 cut(s) 10, 594
Tru1I TTAA 1 cut(s) 702
Tru9I TTAA 1 cut(s) 702
TseFI GTSAC 4 cut(s) 46, 142, 259, 403
TseI GCWGC 1 cut(s) 688
Tsp45I GTSAC 4 cut(s) 46, 142, 259, 403
TspDTI ATGAA 1 cut(s) 17
Tth111I GACNNNGTC 1 cut(s) 44
VpaK11BI GGWCC 2 cut(s) 86, 385
XmiI GTMKAC 2 cut(s) 155, 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.