FvH4_6g35581

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000623
Physical Location & Seq
Reverse (-)
28086226 .. 28088496
2271 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g35581.t1

Sequence Viewer

Length: 438 bp
ATGGCAGACTCGGCCCGATCCTCATCAGATACAATAATTCAATTGCATGGTGAAGAATTTTGGCCTCTTTCAGGAAAACCTTTCTTTGATATAATACTCGCAAAATCACATGTCAAACCTTCTTACATGCTGGGAATTCCAGCCAAACTTCATTCAATACTACCACCTTGTTCAATCCCTACGGTTCTCAACTTTGCGGGAAAAAGTTGGGAGATGACATGTGGTGGAAAACACCGAAAGCACAAGTTGGATAAAGAGTCATGGAAAGCATTTGTTGATGACAACAAGTTAAAGGCTGGCGATGGATGTGTGTTTGAAGTCATGGAGTGCAGCACTACAAAACTAGAATTAAGAGTCCAAATCCTCCGAGGTGACATCCCAGCTGAACTCCTAGAGAAGGAAAGAGGTGATGAAGCCGAGAAACCCATTGTTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

146

Amino Acids

16.18

Weight (kDa)

6.51

Isoelectric Point (pI)

30.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 29 - 123 1e-11 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000623)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g35581 FvH4_6g35581 FvH4_6g35582 FvH4_7g07710 FvH4_7g07720
malus_domestica MD02G1233300.v1.1 MD07G1080300.v1.1
prunus_persica Prupe.2G100300_v2.0.a1 Prupe.2G100300_v2.0.a1 Prupe.2G100300_v2.0.a1 Prupe.2G100300_v2.0.a1 Prupe.2G104300_v2.0.a1 Prupe.2G104700_v2.0.a1 Prupe.2G104700_v2.0.a1 Prupe.2G104700_v2.0.a1
pyrus_communis pycom02g20150 pycom07g06390
rosa_chinensis RchiOBHm_Chr1g0342371 RchiOBHm_Chr1g0342381 RchiOBHm_Chr1g0342401 RchiOBHm_Chr1g0342491 RchiOBHm_Chr1g0342511 RchiOBHm_Chr2g0147401 RchiOBHm_Chr2g0147411
rosa_laevigata RLG00000020253 RLG00000020259 RLG00000029157 RLG00000029158
rosa_multiflora Rmu_sc0000979.1_g000010 Rmu_sc0001159.1_g000034 Rmu_sc0001159.1_g000035 Rmu_sc0005388.1_g000001 Rmu_sc0009535.1_g000001 Rmu_sc0009535.1_g000003 Rmu_sc0011976.1_g000004 Rmu_sc0011976.1_g000005 Rmu_sc0027133.1_g000002
rosa_roxburghii Rroxscaffold_2G00099550 Rroxscaffold_2G00099560 Rroxscaffold_4G00296180 Rroxscaffold_4G00312990 Rroxscaffold_4G00313010 Rroxscaffold_4G00313030 Rroxscaffold_4G00313060
rosa_rugosa Rorug01G0150600.1 Rorug01G0150700.1 Rorug01G0150800.1 Rorug01G0150900.1 Rorug01G0151000.1 Rorug01G0151100.1 Rorug01G0151200.1 Rorug01G0151300.1 Rorug01G0151400.1 Rorug02G0399500 Rorug02G0399600 Rorug02G0399700 Rorug02G0399800 Rorug02G0399800 Rorug02G0399900
rosa_samantha Rh1AG165800 Rh1AG165900 Rh1BG132300 Rh1BG132400 Rh1CG154400 Rh1CG154500 Rh1DG167200 Rh1DG167300 Rh2AG456600 Rh2AG456700 Rh2BG469100 Rh2CG444100 Rh2CG444200 Rh2DG478400 Rh2DG478500
rosa_wichuraiana Rw1G013810 Rw1G013820 Rw1G013860 Rw2G037370 Rw2G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 197
AclWI GGATC 1 cut(s) 12
AcsI RAATTY 2 cut(s) 56, 135
AfiI CCNNNNNNNGG 2 cut(s) 71, 397
AflIII ACRYGT 2 cut(s) 109, 218
AgsI TTSAA 4 cut(s) 41, 156, 174, 317
AluBI AGCT 1 cut(s) 383
AluI AGCT 1 cut(s) 383
AlwI GGATC 1 cut(s) 12
AoxI GGCC 2 cut(s) 12, 62
ApeKI GCWGC 1 cut(s) 330
ApoI RAATTY 2 cut(s) 56, 135
AspS9I GGNCC 1 cut(s) 13
AsuHPI GGTGA 3 cut(s) 62, 383, 419
BbvI GCAGC 1 cut(s) 342
BccI CCATC 1 cut(s) 296
BfaI CTAG 2 cut(s) 344, 392
BisI GCNGC 1 cut(s) 331
BlsI GCNGC 1 cut(s) 332
BmgT120I GGNCC 1 cut(s) 13
BsaBI GATNNNNATC 1 cut(s) 22
BsaJI CCNNGG 1 cut(s) 367
Bsc4I CCNNNNNNNGG 2 cut(s) 71, 397
Bse8I GATNNNNATC 1 cut(s) 22
BseDI CCNNGG 1 cut(s) 367
BseGI GGATG 2 cut(s) 311, 375
BseJI GATNNNNATC 1 cut(s) 22
BseLI CCNNNNNNNGG 2 cut(s) 71, 397
BseXI GCAGC 1 cut(s) 342
BseYI CCCAGC 2 cut(s) 130, 379
BsgI GTGCAG 1 cut(s) 349
BshFI GGCC 2 cut(s) 14, 64
BslI CCNNNNNNNGG 2 cut(s) 71, 397
BsnI GGCC 2 cut(s) 14, 64
Bsp143I GATC 1 cut(s) 17
BspACI CCGC 1 cut(s) 197
BspANI GGCC 2 cut(s) 14, 64
BspPI GGATC 1 cut(s) 12
BssECI CCNNGG 1 cut(s) 367
BssMI GATC 1 cut(s) 17
Bst4CI ACNGT 1 cut(s) 184
BstC8I GCNNGC 1 cut(s) 298
BstENI CCTNNNNNAGG 2 cut(s) 69, 395
BstF5I GGATG 2 cut(s) 311, 375
BstKTI GATC 1 cut(s) 20
BstMBI GATC 1 cut(s) 17
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNSI RCATGY 3 cut(s) 113, 130, 222
BstV1I GCAGC 1 cut(s) 342
BsuRI GGCC 2 cut(s) 14, 64
BtgZI GCGATG 1 cut(s) 315
BtsCI GGATG 2 cut(s) 311, 375
Cac8I GCNNGC 1 cut(s) 298
Cfr13I GGNCC 1 cut(s) 13
CviAII CATG 6 cut(s) 47, 110, 127, 219, 261, 322
CviJI RGCY 6 cut(s) 14, 64, 143, 296, 383, 416
CviKI_1 RGCY 6 cut(s) 14, 64, 143, 296, 383, 416
DpnI GATC 1 cut(s) 19
DpnII GATC 1 cut(s) 17
EcoNI CCTNNNNNAGG 2 cut(s) 69, 395
EcoRI GAATTC 1 cut(s) 135
FaeI CATG 6 cut(s) 50, 113, 130, 222, 264, 325
FaiI YATR 7 cut(s) 48, 92, 111, 128, 220, 262, 323
FatI CATG 6 cut(s) 46, 109, 126, 218, 260, 321
FauI CCCGC 1 cut(s) 190
Fnu4HI GCNGC 1 cut(s) 331
FokI GGATG 2 cut(s) 318, 362
Fsp4HI GCNGC 1 cut(s) 331
FspBI CTAG 2 cut(s) 344, 392
GluI GCNGC 1 cut(s) 331
GsaI CCCAGC 2 cut(s) 134, 383
HaeIII GGCC 2 cut(s) 14, 64
Hin1II CATG 6 cut(s) 50, 113, 130, 222, 264, 325
HinfI GANTC 3 cut(s) 8, 257, 354
HphI GGTGA 3 cut(s) 62, 383, 419
Hpy188I TCNGA 2 cut(s) 28, 368
Hpy188III TCNNGA 1 cut(s) 72
HpyAV CCTTC 2 cut(s) 129, 391
HpyCH4III ACNGT 1 cut(s) 184
HpyCH4V TGCA 2 cut(s) 46, 330
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
Hsp92II CATG 6 cut(s) 50, 113, 130, 222, 264, 325
Kzo9I GATC 1 cut(s) 17
LpnPI CCDG 5 cut(s) 57, 116, 153, 282, 393
Lsp1109I GCAGC 1 cut(s) 342
MaeI CTAG 2 cut(s) 344, 392
MaeIII GTNAC 1 cut(s) 371
MalI GATC 1 cut(s) 19
MboI GATC 1 cut(s) 17
MboII GAAGA 1 cut(s) 65
MfeI CAATTG 1 cut(s) 41
MluCI AATT 5 cut(s) 36, 41, 56, 135, 347
MlyI GAGTC 3 cut(s) 2, 266, 363
MmeI TCCRAC 1 cut(s) 228
MnlI CCTC 5 cut(s) 31, 75, 362, 374, 398
MseI TTAA 2 cut(s) 290, 350
MspA1I CMGCKG 1 cut(s) 383
MunI CAATTG 1 cut(s) 41
MwoI GCNNNNNNNGC 1 cut(s) 11
NdeII GATC 1 cut(s) 17
NlaIII CATG 6 cut(s) 50, 113, 130, 222, 264, 325
NmuCI GTSAC 1 cut(s) 371
NspI RCATGY 3 cut(s) 113, 130, 222
PciI ACATGT 2 cut(s) 109, 218
PkrI GCNGC 1 cut(s) 332
PleI GAGTC 3 cut(s) 2, 265, 362
PpsI GAGTC 3 cut(s) 2, 265, 362
PscI ACATGT 2 cut(s) 109, 218
PspFI CCCAGC 2 cut(s) 130, 379
PspPI GGNCC 1 cut(s) 13
PvuII CAGCTG 1 cut(s) 383
SaqAI TTAA 2 cut(s) 290, 350
SatI GCNGC 1 cut(s) 331
Sau3AI GATC 1 cut(s) 17
Sau96I GGNCC 1 cut(s) 13
SchI GAGTC 3 cut(s) 2, 266, 363
SetI ASST 6 cut(s) 82, 121, 169, 373, 385, 409
Sse9I AATT 5 cut(s) 36, 41, 56, 135, 347
SsiI CCGC 1 cut(s) 197
SspMI CTAG 2 cut(s) 344, 392
TaaI ACNGT 1 cut(s) 184
TasI AATT 5 cut(s) 36, 41, 56, 135, 347
Tru1I TTAA 2 cut(s) 290, 350
Tru9I TTAA 2 cut(s) 290, 350
TseFI GTSAC 1 cut(s) 371
TseI GCWGC 1 cut(s) 330
Tsp45I GTSAC 1 cut(s) 371
TspDTI ATGAA 2 cut(s) 140, 426
XagI CCTNNNNNAGG 2 cut(s) 69, 395
XapI RAATTY 2 cut(s) 56, 135
XceI RCATGY 3 cut(s) 113, 130, 222
XspI CTAG 2 cut(s) 344, 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.