Rh2BG469100

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000623
Physical Location & Seq
Reverse (-)
66263694 .. 66268097
4404 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG469100.1

Sequence Viewer

Length: 429 bp
ATGGCCAAATCTTCATCGAATCCTGTAGTTGAATTGAATGGAGAGGAATTCTGGCCACTTTCTGGGAAACCATACTTTGACGTCATACTCACGAAATCACATGTGAAACCCATTTACCAACTGGGGATCCCAGCCAAACTTGAACCAGTACTACCCTCTGGTTCAATACATACAGTTCTCACATTTGGGGGTAAGAGTTGGGAGATGACCTATAATGGAGAAAAACGTCATAAACAATTCGATCGAAAGTCATGGGGAGCATTTGTCGATGAAAACAATTTGAAGTCTGGAGATGGATGCGTGTTTGAAGTCATGGAGTGCAGCAGTACAAAACTAGTATTAAGAGTCCAAATCCTCCGAGGTGACATCCCAACTGAACTTTTAGAGAAGGAGAAAGGTGACGAAGCCGAGAAACCCATTATTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

142

Amino Acids

15.91

Weight (kDa)

5.94

Isoelectric Point (pI)

32.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 26 - 116 1.2e-10 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000623)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g35581 FvH4_6g35581 FvH4_6g35582 FvH4_7g07710 FvH4_7g07720
malus_domestica MD02G1233300.v1.1 MD07G1080300.v1.1
prunus_persica Prupe.2G100300_v2.0.a1 Prupe.2G100300_v2.0.a1 Prupe.2G100300_v2.0.a1 Prupe.2G100300_v2.0.a1 Prupe.2G104300_v2.0.a1 Prupe.2G104700_v2.0.a1 Prupe.2G104700_v2.0.a1 Prupe.2G104700_v2.0.a1
pyrus_communis pycom02g20150 pycom07g06390
rosa_chinensis RchiOBHm_Chr1g0342371 RchiOBHm_Chr1g0342381 RchiOBHm_Chr1g0342401 RchiOBHm_Chr1g0342491 RchiOBHm_Chr1g0342511 RchiOBHm_Chr2g0147401 RchiOBHm_Chr2g0147411
rosa_laevigata RLG00000020253 RLG00000020259 RLG00000029157 RLG00000029158
rosa_multiflora Rmu_sc0000979.1_g000010 Rmu_sc0001159.1_g000034 Rmu_sc0001159.1_g000035 Rmu_sc0005388.1_g000001 Rmu_sc0009535.1_g000001 Rmu_sc0009535.1_g000003 Rmu_sc0011976.1_g000004 Rmu_sc0011976.1_g000005 Rmu_sc0027133.1_g000002
rosa_roxburghii Rroxscaffold_2G00099550 Rroxscaffold_2G00099560 Rroxscaffold_4G00296180 Rroxscaffold_4G00312990 Rroxscaffold_4G00313010 Rroxscaffold_4G00313030 Rroxscaffold_4G00313060
rosa_rugosa Rorug01G0150600.1 Rorug01G0150700.1 Rorug01G0150800.1 Rorug01G0150900.1 Rorug01G0151000.1 Rorug01G0151100.1 Rorug01G0151200.1 Rorug01G0151300.1 Rorug01G0151400.1 Rorug02G0399500 Rorug02G0399600 Rorug02G0399700 Rorug02G0399800 Rorug02G0399800 Rorug02G0399900
rosa_samantha Rh1AG165800 Rh1AG165900 Rh1BG132300 Rh1BG132400 Rh1CG154400 Rh1CG154500 Rh1DG167200 Rh1DG167300 Rh2AG456600 Rh2AG456700 Rh2BG469100 Rh2CG444100 Rh2CG444200 Rh2DG478400 Rh2DG478500
rosa_wichuraiana Rw1G013810 Rw1G013820 Rw1G013860 Rw2G037370 Rw2G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 84
AccB7I CCANNNNNTGG 1 cut(s) 62
AclWI GGATC 2 cut(s) 121, 134
AcoI YGGCCR 2 cut(s) 3, 53
AcsI RAATTY 1 cut(s) 47
AcyI GRCGYC 1 cut(s) 81
AfaI GTAC 2 cut(s) 150, 328
AfiI CCNNNNNNNGG 1 cut(s) 62
AflIII ACRYGT 1 cut(s) 100
AgsI TTSAA 6 cut(s) 32, 37, 143, 165, 283, 308
AhlI ACTAGT 1 cut(s) 334
AlwI GGATC 2 cut(s) 121, 134
AoxI GGCC 2 cut(s) 3, 53
ApeKI GCWGC 1 cut(s) 321
ApoI RAATTY 1 cut(s) 47
AsuHPI GGTGA 2 cut(s) 374, 410
BalI TGGCCA 2 cut(s) 5, 55
BamHI GGATCC 1 cut(s) 126
BbvI GCAGC 1 cut(s) 333
BccI CCATC 1 cut(s) 287
BcuI ACTAGT 1 cut(s) 334
BfaI CTAG 1 cut(s) 335
BfmI CTRYAG 1 cut(s) 24
BisI GCNGC 1 cut(s) 322
BlsI GCNGC 1 cut(s) 323
BmcAI AGTACT 1 cut(s) 150
BmiI GGNNCC 1 cut(s) 128
BmrI ACTGGG 1 cut(s) 131
BmsI GCATC 1 cut(s) 287
BmuI ACTGGG 1 cut(s) 131
BpmI CTGGAG 1 cut(s) 309
BsaHI GRCGYC 1 cut(s) 81
BsaJI CCNNGG 1 cut(s) 358
BsaXI ACNNNNNCTCC 2 cut(s) 249, 279
Bsc4I CCNNNNNNNGG 1 cut(s) 62
Bse1I ACTGG 2 cut(s) 126, 146
BseDI CCNNGG 1 cut(s) 358
BseGI GGATG 2 cut(s) 302, 366
BseLI CCNNNNNNNGG 1 cut(s) 62
BseNI ACTGG 2 cut(s) 126, 146
BseXI GCAGC 1 cut(s) 333
BseYI CCCAGC 1 cut(s) 130
BsgI GTGCAG 1 cut(s) 340
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 2 cut(s) 5, 55
BsiEI CGRYCG 1 cut(s) 244
BslI CCNNNNNNNGG 1 cut(s) 62
BsnI GGCC 2 cut(s) 5, 55
Bsp143I GATC 2 cut(s) 126, 241
BspANI GGCC 2 cut(s) 5, 55
BspLI GGNNCC 1 cut(s) 128
BspPI GGATC 2 cut(s) 121, 134
BsrI ACTGG 2 cut(s) 126, 146
BssECI CCNNGG 1 cut(s) 358
BssMI GATC 2 cut(s) 126, 241
BssNI GRCGYC 1 cut(s) 81
Bst4CI ACNGT 1 cut(s) 175
BstACI GRCGYC 1 cut(s) 81
BstF5I GGATG 2 cut(s) 302, 366
BstKTI GATC 2 cut(s) 129, 244
BstMBI GATC 2 cut(s) 126, 241
BstMCI CGRYCG 1 cut(s) 244
BstNSI RCATGY 1 cut(s) 104
BstSFI CTRYAG 1 cut(s) 24
BstV1I GCAGC 1 cut(s) 333
BstX2I RGATCY 1 cut(s) 126
BstYI RGATCY 1 cut(s) 126
BsuRI GGCC 2 cut(s) 5, 55
BtsCI GGATG 2 cut(s) 302, 366
Csp6I GTAC 2 cut(s) 149, 327
CviAII CATG 3 cut(s) 101, 252, 313
CviJI RGCY 4 cut(s) 5, 55, 134, 407
CviKI_1 RGCY 4 cut(s) 5, 55, 134, 407
CviQI GTAC 2 cut(s) 149, 327
DpnI GATC 2 cut(s) 128, 243
DpnII GATC 2 cut(s) 126, 241
EaeI YGGCCR 2 cut(s) 3, 53
EcoRI GAATTC 1 cut(s) 47
FaeI CATG 3 cut(s) 104, 255, 316
FaiI YATR 9 cut(s) 73, 86, 102, 171, 213, 231, 253, 314, 427
FatI CATG 3 cut(s) 100, 251, 312
Fnu4HI GCNGC 1 cut(s) 322
FokI GGATG 2 cut(s) 309, 353
Fsp4HI GCNGC 1 cut(s) 322
FspBI CTAG 1 cut(s) 335
GluI GCNGC 1 cut(s) 322
GsaI CCCAGC 1 cut(s) 134
GsuI CTGGAG 1 cut(s) 309
HaeIII GGCC 2 cut(s) 5, 55
Hin1I GRCGYC 1 cut(s) 81
Hin1II CATG 3 cut(s) 104, 255, 316
HinfI GANTC 2 cut(s) 19, 345
HphI GGTGA 2 cut(s) 374, 410
Hpy188I TCNGA 1 cut(s) 359
Hpy188III TCNNGA 2 cut(s) 91, 288
HpyAV CCTTC 1 cut(s) 382
HpyCH4III ACNGT 1 cut(s) 175
HpyCH4IV ACGT 2 cut(s) 81, 226
HpyCH4V TGCA 1 cut(s) 321
HpySE526I ACGT 2 cut(s) 81, 226
Hsp92I GRCGYC 1 cut(s) 81
Hsp92II CATG 3 cut(s) 104, 255, 316
Kzo9I GATC 2 cut(s) 126, 241
LmnI GCTCC 1 cut(s) 257
LpnPI CCDG 8 cut(s) 36, 37, 48, 107, 144, 144, 159, 273
Lsp1109I GCAGC 1 cut(s) 333
LweI GCATC 1 cut(s) 287
MaeI CTAG 1 cut(s) 335
MaeII ACGT 2 cut(s) 81, 226
MaeIII GTNAC 2 cut(s) 362, 398
MalI GATC 2 cut(s) 128, 243
MboI GATC 2 cut(s) 126, 241
MboII GAAGA 1 cut(s) 3
MflI RGATCY 1 cut(s) 126
MlsI TGGCCA 2 cut(s) 5, 55
MluCI AATT 4 cut(s) 32, 47, 236, 277
MluNI TGGCCA 2 cut(s) 5, 55
MlyI GAGTC 1 cut(s) 354
MnlI CCTC 4 cut(s) 37, 166, 353, 365
Mox20I TGGCCA 2 cut(s) 5, 55
MscI TGGCCA 2 cut(s) 5, 55
MseI TTAA 1 cut(s) 341
Msp20I TGGCCA 2 cut(s) 5, 55
NdeII GATC 2 cut(s) 126, 241
NlaIII CATG 3 cut(s) 104, 255, 316
NlaIV GGNNCC 1 cut(s) 128
NmuCI GTSAC 2 cut(s) 362, 398
NspI RCATGY 1 cut(s) 104
PciI ACATGT 1 cut(s) 100
PfeI GAWTC 1 cut(s) 19
PflMI CCANNNNNTGG 1 cut(s) 62
PkrI GCNGC 1 cut(s) 323
Ple19I CGATCG 1 cut(s) 244
PleI GAGTC 1 cut(s) 353
PpsI GAGTC 1 cut(s) 353
PscI ACATGT 1 cut(s) 100
PspFI CCCAGC 1 cut(s) 130
PspN4I GGNNCC 1 cut(s) 128
PsrI GAACNNNNNNTAC 2 cut(s) 135, 167
PsuI RGATCY 1 cut(s) 126
PvuI CGATCG 1 cut(s) 244
RsaI GTAC 2 cut(s) 150, 328
RsaNI GTAC 2 cut(s) 149, 327
SaqAI TTAA 1 cut(s) 341
SatI GCNGC 1 cut(s) 322
Sau3AI GATC 2 cut(s) 126, 241
ScaI AGTACT 1 cut(s) 150
SchI GAGTC 1 cut(s) 354
SetI ASST 5 cut(s) 84, 212, 229, 364, 400
SfaNI GCATC 1 cut(s) 287
SfcI CTRYAG 1 cut(s) 24
SpeI ACTAGT 1 cut(s) 334
Sse9I AATT 4 cut(s) 32, 47, 236, 277
SspMI CTAG 1 cut(s) 335
TaaI ACNGT 1 cut(s) 175
TaiI ACGT 2 cut(s) 84, 229
TaqI TCGA 4 cut(s) 17, 240, 244, 267
TasI AATT 4 cut(s) 32, 47, 236, 277
TatI WGTACW 2 cut(s) 148, 326
TfiI GAWTC 1 cut(s) 19
Tru1I TTAA 1 cut(s) 341
Tru9I TTAA 1 cut(s) 341
TseFI GTSAC 2 cut(s) 362, 398
TseI GCWGC 1 cut(s) 321
Tsp45I GTSAC 2 cut(s) 362, 398
TspDTI ATGAA 1 cut(s) 285
Van91I CCANNNNNTGG 1 cut(s) 62
XapI RAATTY 1 cut(s) 47
XceI RCATGY 1 cut(s) 104
XcmI CCANNNNNNNNNTGG 1 cut(s) 118
XspI CTAG 1 cut(s) 335
ZraI GACGTC 1 cut(s) 82
ZrmI AGTACT 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.