MD02G1233300.v1.1

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000623
Physical Location & Seq
Reverse (-)
27961152 .. 27964204
3053 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1233300.v1.1.491

Sequence Viewer

Length: 432 bp
ATGGGAGACCAACCCACATCAAATCCCCCGATTGAATTGCAAGGGGATGAGTTTTGGCCGCTTTCAGAGAAACCCTTTTTTGAAGTCGTTATCGCAAAAGCAAATGTCAAGCCCTCTTATCAAATGGTGATCCCGGCCAAATTTCAACAAACACTACCTTCCTGTTCAATTCATACGGTTCTCATGTTTGGGGGCAAAAACTGGGAGATGACATATACATGCGGATCTGGTCAGAGAAAGTTCGATACTAACTGGAGAGAATTTGTCAATGACAACAATTTGAAGGTTGGAGATGCATGTGTATTCGAACTCCTGGAGTGCAGCAGCACAAAACTCATATTCAGAGTCCAAATTCTCAGAGGTGATATCCCATCTGAACTTCTACGCAAGCTGAAAGGTGAGACTGTAGATGCACCAATAATTATTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

144

Amino Acids

16.19

Weight (kDa)

5.21

Isoelectric Point (pI)

33.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 27 - 116 4.1e-14 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000623)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g35581 FvH4_6g35581 FvH4_6g35582 FvH4_7g07710 FvH4_7g07720
malus_domestica MD02G1233300.v1.1 MD07G1080300.v1.1
prunus_persica Prupe.2G100300_v2.0.a1 Prupe.2G100300_v2.0.a1 Prupe.2G100300_v2.0.a1 Prupe.2G100300_v2.0.a1 Prupe.2G104300_v2.0.a1 Prupe.2G104700_v2.0.a1 Prupe.2G104700_v2.0.a1 Prupe.2G104700_v2.0.a1
pyrus_communis pycom02g20150 pycom07g06390
rosa_chinensis RchiOBHm_Chr1g0342371 RchiOBHm_Chr1g0342381 RchiOBHm_Chr1g0342401 RchiOBHm_Chr1g0342491 RchiOBHm_Chr1g0342511 RchiOBHm_Chr2g0147401 RchiOBHm_Chr2g0147411
rosa_laevigata RLG00000020253 RLG00000020259 RLG00000029157 RLG00000029158
rosa_multiflora Rmu_sc0000979.1_g000010 Rmu_sc0001159.1_g000034 Rmu_sc0001159.1_g000035 Rmu_sc0005388.1_g000001 Rmu_sc0009535.1_g000001 Rmu_sc0009535.1_g000003 Rmu_sc0011976.1_g000004 Rmu_sc0011976.1_g000005 Rmu_sc0027133.1_g000002
rosa_roxburghii Rroxscaffold_2G00099550 Rroxscaffold_2G00099560 Rroxscaffold_4G00296180 Rroxscaffold_4G00312990 Rroxscaffold_4G00313010 Rroxscaffold_4G00313030 Rroxscaffold_4G00313060
rosa_rugosa Rorug01G0150600.1 Rorug01G0150700.1 Rorug01G0150800.1 Rorug01G0150900.1 Rorug01G0151000.1 Rorug01G0151100.1 Rorug01G0151200.1 Rorug01G0151300.1 Rorug01G0151400.1 Rorug02G0399500 Rorug02G0399600 Rorug02G0399700 Rorug02G0399800 Rorug02G0399800 Rorug02G0399900
rosa_samantha Rh1AG165800 Rh1AG165900 Rh1BG132300 Rh1BG132400 Rh1CG154400 Rh1CG154500 Rh1DG167200 Rh1DG167300 Rh2AG456600 Rh2AG456700 Rh2BG469100 Rh2CG444100 Rh2CG444200 Rh2DG478400 Rh2DG478500
rosa_wichuraiana Rw1G013810 Rw1G013820 Rw1G013860 Rw2G037370 Rw2G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 59, 222
AclWI GGATC 2 cut(s) 124, 232
AcoI YGGCCR 2 cut(s) 56, 135
AcsI RAATTY 3 cut(s) 140, 260, 351
AgsI TTSAA 5 cut(s) 35, 83, 146, 168, 283
AjnI CCWGG 1 cut(s) 312
AluBI AGCT 1 cut(s) 391
AluI AGCT 1 cut(s) 391
Alw26I GTCTC 1 cut(s) 395
AlwI GGATC 2 cut(s) 124, 232
AoxI GGCC 2 cut(s) 56, 135
ApeKI GCWGC 2 cut(s) 321, 324
ApoI RAATTY 3 cut(s) 140, 260, 351
AsuC2I CCSGG 1 cut(s) 134
AsuHPI GGTGA 3 cut(s) 139, 374, 410
AsuII TTCGAA 1 cut(s) 306
BbvI GCAGC 2 cut(s) 333, 336
BccI CCATC 1 cut(s) 379
BcgI CGANNNNNNTGC 2 cut(s) 19, 53
BciT130I CCWGG 1 cut(s) 314
BcnI CCSGG 1 cut(s) 134
BcoDI GTCTC 1 cut(s) 395
BfmI CTRYAG 1 cut(s) 405
BisI GCNGC 3 cut(s) 59, 322, 325
BlsI GCNGC 3 cut(s) 60, 323, 326
Bme1390I CCNGG 2 cut(s) 134, 314
BmrFI CCNGG 2 cut(s) 134, 314
BmrI ACTGGG 1 cut(s) 211
BmsI GCATC 2 cut(s) 283, 400
BmuI ACTGGG 1 cut(s) 211
BpmI CTGGAG 2 cut(s) 274, 335
Bpu14I TTCGAA 1 cut(s) 306
BpuMI CCSGG 1 cut(s) 134
Bse1I ACTGG 2 cut(s) 206, 257
BseBI CCWGG 1 cut(s) 314
BseGI GGATG 1 cut(s) 52
BseMII CTCAG 1 cut(s) 370
BseNI ACTGG 2 cut(s) 206, 257
BseXI GCAGC 2 cut(s) 333, 336
BsgI GTGCAG 1 cut(s) 340
BshFI GGCC 2 cut(s) 58, 137
BsiSI CCGG 1 cut(s) 134
BsmAI GTCTC 1 cut(s) 395
BsnI GGCC 2 cut(s) 58, 137
Bsp119I TTCGAA 1 cut(s) 306
Bsp143I GATC 2 cut(s) 129, 224
BspACI CCGC 2 cut(s) 59, 222
BspANI GGCC 2 cut(s) 58, 137
BspCNI CTCAG 1 cut(s) 369
BspPI GGATC 2 cut(s) 124, 232
BspT104I TTCGAA 1 cut(s) 306
BsrI ACTGG 2 cut(s) 206, 257
BssMI GATC 2 cut(s) 129, 224
Bst2UI CCWGG 1 cut(s) 314
Bst4CI ACNGT 2 cut(s) 178, 406
BstBI TTCGAA 1 cut(s) 306
BstC8I GCNNGC 1 cut(s) 389
BstDEI CTNAG 1 cut(s) 356
BstF5I GGATG 1 cut(s) 52
BstKTI GATC 2 cut(s) 132, 227
BstMAI GTCTC 1 cut(s) 395
BstMBI GATC 2 cut(s) 129, 224
BstNI CCWGG 1 cut(s) 314
BstNSI RCATGY 2 cut(s) 222, 300
BstSCI CCNGG 2 cut(s) 132, 312
BstSFI CTRYAG 1 cut(s) 405
BstV1I GCAGC 2 cut(s) 333, 336
BstX2I RGATCY 1 cut(s) 224
BstYI RGATCY 1 cut(s) 224
BsuRI GGCC 2 cut(s) 58, 137
BtsCI GGATG 1 cut(s) 52
Cac8I GCNNGC 1 cut(s) 389
CviAII CATG 3 cut(s) 184, 219, 297
CviJI RGCY 4 cut(s) 58, 112, 137, 391
CviKI_1 RGCY 4 cut(s) 58, 112, 137, 391
DdeI CTNAG 1 cut(s) 356
DpnI GATC 2 cut(s) 131, 226
DpnII GATC 2 cut(s) 129, 224
EaeI YGGCCR 2 cut(s) 56, 135
Eco32I GATATC 1 cut(s) 367
EcoRII CCWGG 1 cut(s) 312
EcoRV GATATC 1 cut(s) 367
EcoT22I ATGCAT 1 cut(s) 298
FaeI CATG 3 cut(s) 187, 222, 300
FaiI YATR 7 cut(s) 174, 185, 214, 216, 220, 298, 338
FatI CATG 3 cut(s) 183, 218, 296
Fnu4HI GCNGC 3 cut(s) 59, 322, 325
FokI GGATG 1 cut(s) 59
Fsp4HI GCNGC 3 cut(s) 59, 322, 325
GluI GCNGC 3 cut(s) 59, 322, 325
GsuI CTGGAG 2 cut(s) 274, 335
HaeIII GGCC 2 cut(s) 58, 137
HapII CCGG 1 cut(s) 134
Hin1II CATG 3 cut(s) 187, 222, 300
HinfI GANTC 1 cut(s) 345
HpaII CCGG 1 cut(s) 134
HphI GGTGA 3 cut(s) 139, 374, 410
Hpy188I TCNGA 5 cut(s) 67, 234, 344, 359, 376
HpyAV CCTTC 2 cut(s) 168, 277
HpyCH4III ACNGT 2 cut(s) 178, 406
HpyCH4V TGCA 4 cut(s) 40, 296, 321, 413
HpyF3I CTNAG 1 cut(s) 356
Hsp92II CATG 3 cut(s) 187, 222, 300
Kzo9I GATC 2 cut(s) 129, 224
LpnPI CCDG 7 cut(s) 147, 175, 187, 213, 238, 299, 326
Lsp1109I GCAGC 2 cut(s) 333, 336
LweI GCATC 2 cut(s) 283, 400
MalI GATC 2 cut(s) 131, 226
MboI GATC 2 cut(s) 129, 224
MflI RGATCY 1 cut(s) 224
MluCI AATT 7 cut(s) 35, 140, 168, 260, 277, 351, 420
MlyI GAGTC 1 cut(s) 354
MmeI TCCRAC 1 cut(s) 268
MnlI CCTC 2 cut(s) 124, 353
Mph1103I ATGCAT 1 cut(s) 298
MslI CAYNNNNRTG 1 cut(s) 217
MspI CCGG 1 cut(s) 134
MspR9I CCNGG 2 cut(s) 134, 314
MvaI CCWGG 1 cut(s) 314
NciI CCSGG 1 cut(s) 134
NdeII GATC 2 cut(s) 129, 224
NlaIII CATG 3 cut(s) 187, 222, 300
NsiI ATGCAT 1 cut(s) 298
NspI RCATGY 2 cut(s) 222, 300
NspV TTCGAA 1 cut(s) 306
PfoI TCCNGGA 1 cut(s) 312
PkrI GCNGC 3 cut(s) 60, 323, 326
PleI GAGTC 1 cut(s) 353
PpsI GAGTC 1 cut(s) 353
Psp6I CCWGG 1 cut(s) 312
PspGI CCWGG 1 cut(s) 312
PsuI RGATCY 1 cut(s) 224
RseI CAYNNNNRTG 1 cut(s) 217
SatI GCNGC 3 cut(s) 59, 322, 325
Sau3AI GATC 2 cut(s) 129, 224
SchI GAGTC 1 cut(s) 354
ScrFI CCNGG 2 cut(s) 134, 314
SetI ASST 5 cut(s) 160, 288, 364, 393, 400
SfaNI GCATC 2 cut(s) 283, 400
SfcI CTRYAG 1 cut(s) 405
SfuI TTCGAA 1 cut(s) 306
SmiMI CAYNNNNRTG 1 cut(s) 217
Sse9I AATT 7 cut(s) 35, 140, 168, 260, 277, 351, 420
SsiI CCGC 2 cut(s) 59, 222
StyD4I CCNGG 2 cut(s) 132, 312
TaaI ACNGT 2 cut(s) 178, 406
TaqI TCGA 2 cut(s) 243, 306
TasI AATT 7 cut(s) 35, 140, 168, 260, 277, 351, 420
TauI GCSGC 1 cut(s) 61
TseI GCWGC 2 cut(s) 321, 324
TspDTI ATGAA 1 cut(s) 161
XapI RAATTY 3 cut(s) 140, 260, 351
XceI RCATGY 2 cut(s) 222, 300
Zsp2I ATGCAT 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.