RLG00000020253

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000623
Physical Location & Seq
Reverse (-)
62894182 .. 62896206
2025 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020253

Sequence Viewer

Length: 594 bp
ATGTGGGTTTTAAAGTTTGGTTCACAGGAATCCTGGTTTCATGATTCTCTGTGCTTAAGTTGCTCTCCAAGGCAACTTTCACCAGATATTATCCTCACAGCTTTTTCGGTTTCAAATAAAGAATTGCAATTTGGCAAAGCAGAAGATCCTGAAATGGCAGAATCGGCTCAATCCTCATCAGATACAACAATTCAATTGCATGGCGAAGAATTTTGGCCTCTTTCAGGAAAACCTTACTTTGATATAATACTAGCAAAAACACAAGTCAAACCGTATTACAAACTGGGGATCCCAGCCAAACTTCATCCAATACTACCATCTTGTTCATTCCCTACGGTTCTCACCTTTGCGGGAAAGAGTTGGGAGATGACATGTGATGGATCAAACAAAAAGCACAAAGGCTTGGACAAAGAGTCATGGAAAGCATTTATTGACGACAACAATTTGAAGGCTGGAGATGGATGCGTGTTTGAAGTCATGGAGTGCAGCAGTATAAAACTAGTATTCAGAGTCCAAATCCTCCGAGGTGACATCCCAGCTGAACTTTTAGAGAAGGAAAAAGGTGACGAAGCCGAGAAACCCATCATTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

198

Amino Acids

22.06

Weight (kDa)

5.29

Isoelectric Point (pI)

43.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 115 - 171 1.3e-07 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000623)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g35581 FvH4_6g35581 FvH4_6g35582 FvH4_7g07710 FvH4_7g07720
malus_domestica MD02G1233300.v1.1 MD07G1080300.v1.1
prunus_persica Prupe.2G100300_v2.0.a1 Prupe.2G100300_v2.0.a1 Prupe.2G100300_v2.0.a1 Prupe.2G100300_v2.0.a1 Prupe.2G104300_v2.0.a1 Prupe.2G104700_v2.0.a1 Prupe.2G104700_v2.0.a1 Prupe.2G104700_v2.0.a1
pyrus_communis pycom02g20150 pycom07g06390
rosa_chinensis RchiOBHm_Chr1g0342371 RchiOBHm_Chr1g0342381 RchiOBHm_Chr1g0342401 RchiOBHm_Chr1g0342491 RchiOBHm_Chr1g0342511 RchiOBHm_Chr2g0147401 RchiOBHm_Chr2g0147411
rosa_laevigata RLG00000020253 RLG00000020259 RLG00000029157 RLG00000029158
rosa_multiflora Rmu_sc0000979.1_g000010 Rmu_sc0001159.1_g000034 Rmu_sc0001159.1_g000035 Rmu_sc0005388.1_g000001 Rmu_sc0009535.1_g000001 Rmu_sc0009535.1_g000003 Rmu_sc0011976.1_g000004 Rmu_sc0011976.1_g000005 Rmu_sc0027133.1_g000002
rosa_roxburghii Rroxscaffold_2G00099550 Rroxscaffold_2G00099560 Rroxscaffold_4G00296180 Rroxscaffold_4G00312990 Rroxscaffold_4G00313010 Rroxscaffold_4G00313030 Rroxscaffold_4G00313060
rosa_rugosa Rorug01G0150600.1 Rorug01G0150700.1 Rorug01G0150800.1 Rorug01G0150900.1 Rorug01G0151000.1 Rorug01G0151100.1 Rorug01G0151200.1 Rorug01G0151300.1 Rorug01G0151400.1 Rorug02G0399500 Rorug02G0399600 Rorug02G0399700 Rorug02G0399800 Rorug02G0399800 Rorug02G0399900
rosa_samantha Rh1AG165800 Rh1AG165900 Rh1BG132300 Rh1BG132400 Rh1CG154400 Rh1CG154500 Rh1DG167200 Rh1DG167300 Rh2AG456600 Rh2AG456700 Rh2BG469100 Rh2CG444100 Rh2CG444200 Rh2DG478400 Rh2DG478500
rosa_wichuraiana Rw1G013810 Rw1G013820 Rw1G013860 Rw2G037370 Rw2G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 350
AclWI GGATC 4 cut(s) 140, 283, 296, 388
AcsI RAATTY 1 cut(s) 209
AfiI CCNNNNNNNGG 1 cut(s) 224
AflII CTTAAG 1 cut(s) 55
AflIII ACRYGT 1 cut(s) 371
AgsI TTSAA 4 cut(s) 114, 194, 448, 473
AhdI GACNNNNNGTC 1 cut(s) 412
AhlI ACTAGT 1 cut(s) 499
AjnI CCWGG 1 cut(s) 32
AjuI GAANNNNNNNTTGG 4 cut(s) 61, 93, 114, 146
AluBI AGCT 2 cut(s) 101, 539
AluI AGCT 2 cut(s) 101, 539
AlwI GGATC 4 cut(s) 140, 283, 296, 388
AoxI GGCC 1 cut(s) 215
ApeKI GCWGC 1 cut(s) 486
ApoI RAATTY 1 cut(s) 209
AsuHPI GGTGA 4 cut(s) 72, 334, 539, 575
BamHI GGATCC 1 cut(s) 288
BbvI GCAGC 1 cut(s) 498
BccI CCATC 4 cut(s) 325, 371, 452, 590
BciT130I CCWGG 1 cut(s) 34
BcuI ACTAGT 1 cut(s) 499
BfaI CTAG 2 cut(s) 251, 500
BfrI CTTAAG 1 cut(s) 55
BisI GCNGC 1 cut(s) 487
BlsI GCNGC 1 cut(s) 488
Bme1390I CCNGG 1 cut(s) 34
BmeRI GACNNNNNGTC 1 cut(s) 412
BmiI GGNNCC 1 cut(s) 290
BmrFI CCNGG 1 cut(s) 34
BmrI ACTGGG 1 cut(s) 293
BmsI GCATC 1 cut(s) 452
BmuI ACTGGG 1 cut(s) 293
BpmI CTGGAG 1 cut(s) 474
BsaJI CCNNGG 2 cut(s) 68, 523
Bsc4I CCNNNNNNNGG 1 cut(s) 224
Bse1I ACTGG 1 cut(s) 288
BseBI CCWGG 1 cut(s) 34
BseDI CCNNGG 2 cut(s) 68, 523
BseGI GGATG 3 cut(s) 304, 467, 531
BseLI CCNNNNNNNGG 1 cut(s) 224
BseNI ACTGG 1 cut(s) 288
BseXI GCAGC 1 cut(s) 498
BseYI CCCAGC 2 cut(s) 292, 535
BsgI GTGCAG 1 cut(s) 505
BshFI GGCC 1 cut(s) 217
BslI CCNNNNNNNGG 1 cut(s) 224
BsnI GGCC 1 cut(s) 217
Bsp143I GATC 3 cut(s) 145, 288, 380
BspACI CCGC 1 cut(s) 350
BspANI GGCC 1 cut(s) 217
BspHI TCATGA 1 cut(s) 40
BspLI GGNNCC 1 cut(s) 290
BspPI GGATC 4 cut(s) 140, 283, 296, 388
BspTI CTTAAG 1 cut(s) 55
BsrI ACTGG 1 cut(s) 288
BssECI CCNNGG 2 cut(s) 68, 523
BssMI GATC 3 cut(s) 145, 288, 380
BssT1I CCWWGG 1 cut(s) 68
Bst2UI CCWGG 1 cut(s) 34
Bst4CI ACNGT 2 cut(s) 273, 337
BstAFI CTTAAG 1 cut(s) 55
BstENI CCTNNNNNAGG 1 cut(s) 222
BstF5I GGATG 3 cut(s) 304, 467, 531
BstKTI GATC 3 cut(s) 148, 291, 383
BstMBI GATC 3 cut(s) 145, 288, 380
BstMWI GCNNNNNNNGC 2 cut(s) 60, 164
BstNI CCWGG 1 cut(s) 34
BstNSI RCATGY 1 cut(s) 375
BstSCI CCNGG 1 cut(s) 32
BstV1I GCAGC 1 cut(s) 498
BstX2I RGATCY 2 cut(s) 145, 288
BstYI RGATCY 2 cut(s) 145, 288
BsuRI GGCC 1 cut(s) 217
BtsCI GGATG 3 cut(s) 304, 467, 531
CciI TCATGA 1 cut(s) 40
CviAII CATG 5 cut(s) 41, 200, 372, 417, 478
CviJI RGCY 8 cut(s) 101, 167, 217, 296, 402, 452, 539, 572
CviKI_1 RGCY 8 cut(s) 101, 167, 217, 296, 402, 452, 539, 572
DpnI GATC 3 cut(s) 147, 290, 382
DpnII GATC 3 cut(s) 145, 288, 380
DraI TTTAAA 1 cut(s) 12
DriI GACNNNNNGTC 1 cut(s) 412
Eam1105I GACNNNNNGTC 1 cut(s) 412
Eco130I CCWWGG 1 cut(s) 68
EcoNI CCTNNNNNAGG 1 cut(s) 222
EcoRII CCWGG 1 cut(s) 32
EcoT14I CCWWGG 1 cut(s) 68
ErhI CCWWGG 1 cut(s) 68
FaeI CATG 5 cut(s) 44, 203, 375, 420, 481
FaiI YATR 8 cut(s) 42, 201, 245, 373, 418, 479, 494, 592
FatI CATG 5 cut(s) 40, 199, 371, 416, 477
FauI CCCGC 1 cut(s) 343
Fnu4HI GCNGC 1 cut(s) 487
FokI GGATG 3 cut(s) 291, 474, 518
Fsp4HI GCNGC 1 cut(s) 487
FspBI CTAG 2 cut(s) 251, 500
GluI GCNGC 1 cut(s) 487
GsaI CCCAGC 2 cut(s) 296, 539
GsuI CTGGAG 1 cut(s) 474
HaeIII GGCC 1 cut(s) 217
Hin1II CATG 5 cut(s) 44, 203, 375, 420, 481
HinfI GANTC 5 cut(s) 29, 44, 161, 413, 510
HphI GGTGA 4 cut(s) 72, 334, 539, 575
Hpy166II GTNNAC 1 cut(s) 23
Hpy188I TCNGA 3 cut(s) 181, 509, 524
Hpy188III TCNNGA 3 cut(s) 41, 149, 225
Hpy8I GTNNAC 1 cut(s) 23
HpyAV CCTTC 2 cut(s) 442, 547
HpyCH4III ACNGT 2 cut(s) 273, 337
HpyCH4V TGCA 3 cut(s) 127, 199, 486
HpyF10VI GCNNNNNNNGC 2 cut(s) 60, 164
Hsp92II CATG 5 cut(s) 44, 203, 375, 420, 481
Kzo9I GATC 3 cut(s) 145, 288, 380
Lsp1109I GCAGC 1 cut(s) 498
LweI GCATC 1 cut(s) 452
MaeI CTAG 2 cut(s) 251, 500
MaeIII GTNAC 2 cut(s) 527, 563
MalI GATC 3 cut(s) 147, 290, 382
MboI GATC 3 cut(s) 145, 288, 380
MboII GAAGA 2 cut(s) 155, 218
MfeI CAATTG 1 cut(s) 194
MflI RGATCY 2 cut(s) 145, 288
MluCI AATT 6 cut(s) 122, 128, 189, 194, 209, 442
MlyI GAGTC 2 cut(s) 422, 519
MnlI CCTC 5 cut(s) 104, 184, 228, 518, 530
MseI TTAA 2 cut(s) 11, 56
MspA1I CMGCKG 1 cut(s) 539
MspCI CTTAAG 1 cut(s) 55
MspR9I CCNGG 1 cut(s) 34
MunI CAATTG 1 cut(s) 194
MvaI CCWGG 1 cut(s) 34
MwoI GCNNNNNNNGC 2 cut(s) 60, 164
NdeII GATC 3 cut(s) 145, 288, 380
NlaIII CATG 5 cut(s) 44, 203, 375, 420, 481
NlaIV GGNNCC 1 cut(s) 290
NmuCI GTSAC 2 cut(s) 527, 563
NspI RCATGY 1 cut(s) 375
PagI TCATGA 1 cut(s) 40
PciI ACATGT 1 cut(s) 371
PfeI GAWTC 3 cut(s) 29, 44, 161
PkrI GCNGC 1 cut(s) 488
PleI GAGTC 2 cut(s) 421, 518
PpsI GAGTC 2 cut(s) 421, 518
PscI ACATGT 1 cut(s) 371
Psp6I CCWGG 1 cut(s) 32
PspFI CCCAGC 2 cut(s) 292, 535
PspGI CCWGG 1 cut(s) 32
PspN4I GGNNCC 1 cut(s) 290
PsuI RGATCY 2 cut(s) 145, 288
PvuII CAGCTG 1 cut(s) 539
SaqAI TTAA 2 cut(s) 11, 56
SatI GCNGC 1 cut(s) 487
Sau3AI GATC 3 cut(s) 145, 288, 380
SchI GAGTC 2 cut(s) 422, 519
ScrFI CCNGG 1 cut(s) 34
SetI ASST 6 cut(s) 103, 235, 347, 529, 541, 565
SfaNI GCATC 1 cut(s) 452
SmlI CTYRAG 1 cut(s) 55
SmoI CTYRAG 1 cut(s) 55
SpeI ACTAGT 1 cut(s) 499
Sse9I AATT 6 cut(s) 122, 128, 189, 194, 209, 442
SsiI CCGC 1 cut(s) 350
SspMI CTAG 2 cut(s) 251, 500
StyD4I CCNGG 1 cut(s) 32
StyI CCWWGG 1 cut(s) 68
TaaI ACNGT 2 cut(s) 273, 337
TasI AATT 6 cut(s) 122, 128, 189, 194, 209, 442
TfiI GAWTC 3 cut(s) 29, 44, 161
Tru1I TTAA 2 cut(s) 11, 56
Tru9I TTAA 2 cut(s) 11, 56
TseFI GTSAC 2 cut(s) 527, 563
TseI GCWGC 1 cut(s) 486
Tsp45I GTSAC 2 cut(s) 527, 563
TspDTI ATGAA 3 cut(s) 29, 293, 315
Vha464I CTTAAG 1 cut(s) 55
XagI CCTNNNNNAGG 1 cut(s) 222
XapI RAATTY 1 cut(s) 209
XceI RCATGY 1 cut(s) 375
XspI CTAG 2 cut(s) 251, 500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.