B3 domain-containing protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1074 |
| AciI | CCGC | 1 cut(s) | 393 |
| AclWI | GGATC | 4 cut(s) | 588, 994, 1117, 1130 |
| AcsI | RAATTY | 1 cut(s) | 646 |
| AfaI | GTAC | 4 cut(s) | 253, 416, 583, 727 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 1132, 1269 |
| AflIII | ACRYGT | 1 cut(s) | 1276 |
| AgsI | TTSAA | 4 cut(s) | 149, 604, 704, 785 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 213, 245 |
| AluBI | AGCT | 5 cut(s) | 116, 401, 669, 758, 1052 |
| AluI | AGCT | 5 cut(s) | 116, 401, 669, 758, 1052 |
| Alw21I | GWGCWC | 3 cut(s) | 1360, 1410, 1442 |
| Alw26I | GTCTC | 3 cut(s) | 608, 690, 1261 |
| Alw44I | GTGCAC | 2 cut(s) | 1356, 1438 |
| AlwI | GGATC | 4 cut(s) | 588, 994, 1117, 1130 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 95, 365 |
| AoxI | GGCC | 1 cut(s) | 215 |
| ApaLI | GTGCAC | 2 cut(s) | 1356, 1438 |
| ApeKI | GCWGC | 4 cut(s) | 275, 398, 401, 1049 |
| ApoI | RAATTY | 1 cut(s) | 646 |
| AspLEI | GCGC | 1 cut(s) | 1387 |
| AspS9I | GGNCC | 1 cut(s) | 1100 |
| AsuHPI | GGTGA | 1 cut(s) | 544 |
| AvaII | GGWCC | 1 cut(s) | 1100 |
| AxyI | CCTNAGG | 1 cut(s) | 1131 |
| BaeGI | GKGCMC | 2 cut(s) | 1360, 1442 |
| BamHI | GGATCC | 1 cut(s) | 1122 |
| BbsI | GAAGAC | 1 cut(s) | 711 |
| Bbv12I | GWGCWC | 3 cut(s) | 1360, 1410, 1442 |
| BbvCI | CCTCAGC | 1 cut(s) | 428 |
| BbvI | GCAGC | 4 cut(s) | 287, 388, 410, 1061 |
| BccI | CCATC | 2 cut(s) | 563, 1271 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 858, 892 |
| BclI | TGATCA | 4 cut(s) | 349, 384, 549, 1320 |
| BcoDI | GTCTC | 3 cut(s) | 608, 690, 1261 |
| BfaI | CTAG | 5 cut(s) | 123, 591, 720, 896, 1058 |
| BglII | AGATCT | 1 cut(s) | 898 |
| BisI | GCNGC | 4 cut(s) | 276, 399, 402, 1050 |
| BlsI | GCNGC | 4 cut(s) | 277, 400, 403, 1051 |
| Bme18I | GGWCC | 1 cut(s) | 1100 |
| BmgT120I | GGNCC | 1 cut(s) | 1100 |
| BmiI | GGNNCC | 2 cut(s) | 1101, 1124 |
| BmsI | GCATC | 5 cut(s) | 89, 442, 699, 986, 1322 |
| BpiI | GAAGAC | 1 cut(s) | 711 |
| BpmI | CTGGAG | 2 cut(s) | 817, 1027 |
| Bpu10I | CCTNAGC | 2 cut(s) | 35, 428 |
| BpuEI | CTTGAG | 1 cut(s) | 464 |
| BsaBI | GATNNNNATC | 1 cut(s) | 554 |
| BsaJI | CCNNGG | 4 cut(s) | 196, 268, 958, 1419 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 1132, 1269 |
| Bse1I | ACTGG | 4 cut(s) | 394, 657, 893, 1010 |
| Bse21I | CCTNAGG | 1 cut(s) | 1131 |
| Bse8I | GATNNNNATC | 1 cut(s) | 554 |
| BseDI | CCNNGG | 4 cut(s) | 196, 268, 958, 1419 |
| BseGI | GGATG | 3 cut(s) | 433, 552, 1175 |
| BseJI | GATNNNNATC | 1 cut(s) | 554 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 1132, 1269 |
| BseMII | CTCAG | 2 cut(s) | 419, 1145 |
| BseNI | ACTGG | 4 cut(s) | 394, 657, 893, 1010 |
| BseRI | GAGGAG | 1 cut(s) | 1119 |
| BseSI | GKGCMC | 2 cut(s) | 1360, 1442 |
| BseXI | GCAGC | 4 cut(s) | 287, 388, 410, 1061 |
| BseYI | CCCAGC | 1 cut(s) | 1052 |
| BsgI | GTGCAG | 1 cut(s) | 31 |
| BshFI | GGCC | 1 cut(s) | 217 |
| BsiHKAI | GWGCWC | 3 cut(s) | 1360, 1410, 1442 |
| BslFI | GGGAC | 1 cut(s) | 940 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 1132, 1269 |
| BsmAI | GTCTC | 3 cut(s) | 608, 690, 1261 |
| BsmBI | CGTCTC | 1 cut(s) | 690 |
| BsmFI | GGGAC | 1 cut(s) | 940 |
| BsnI | GGCC | 1 cut(s) | 217 |
| Bsp1286I | GDGCHC | 3 cut(s) | 1360, 1410, 1442 |
| Bsp1407I | TGTACA | 1 cut(s) | 414 |
| BspACI | CCGC | 1 cut(s) | 393 |
| BspANI | GGCC | 1 cut(s) | 217 |
| BspCNI | CTCAG | 2 cut(s) | 420, 1144 |
| BspHI | TCATGA | 2 cut(s) | 336, 1161 |
| BspLI | GGNNCC | 2 cut(s) | 1101, 1124 |
| BspPI | GGATC | 4 cut(s) | 588, 994, 1117, 1130 |
| BspQI | GCTCTTC | 1 cut(s) | 1303 |
| BsrGI | TGTACA | 1 cut(s) | 414 |
| BsrI | ACTGG | 4 cut(s) | 394, 657, 893, 1010 |
| BssECI | CCNNGG | 4 cut(s) | 196, 268, 958, 1419 |
| BssT1I | CCWWGG | 4 cut(s) | 196, 268, 958, 1419 |
| Bst4CI | ACNGT | 4 cut(s) | 409, 823, 954, 1255 |
| Bst6I | CTCTTC | 4 cut(s) | 760, 959, 1177, 1303 |
| BstAUI | TGTACA | 1 cut(s) | 414 |
| BstC8I | GCNNGC | 1 cut(s) | 756 |
| BstDEI | CTNAG | 8 cut(s) | 35, 160, 422, 428, 577, 759, 855, 1131 |
| BstF5I | GGATG | 3 cut(s) | 433, 552, 1175 |
| BstHHI | GCGC | 1 cut(s) | 1387 |
| BstMAI | GTCTC | 3 cut(s) | 608, 690, 1261 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 214, 1247 |
| BstNSI | RCATGY | 2 cut(s) | 212, 1280 |
| BstSLI | GKGCMC | 2 cut(s) | 1360, 1442 |
| BstV1I | GCAGC | 4 cut(s) | 287, 388, 410, 1061 |
| BstV2I | GAAGAC | 1 cut(s) | 711 |
| BstX2I | RGATCY | 3 cut(s) | 593, 898, 1122 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 670, 1186 |
| BstYI | RGATCY | 3 cut(s) | 593, 898, 1122 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1131 |
| BsuRI | GGCC | 1 cut(s) | 217 |
| BtsCI | GGATG | 3 cut(s) | 433, 552, 1175 |
| BtsIMutI | CAGTG | 2 cut(s) | 387, 405 |
| Cac8I | GCNNGC | 1 cut(s) | 756 |
| CaiI | CAGNNNCTG | 2 cut(s) | 95, 365 |
| CciI | TCATGA | 2 cut(s) | 336, 1161 |
| CfoI | GCGC | 1 cut(s) | 1387 |
| Cfr13I | GGNCC | 1 cut(s) | 1100 |
| CseI | GACGC | 1 cut(s) | 665 |
| Csp6I | GTAC | 4 cut(s) | 252, 415, 582, 726 |
| CviQI | GTAC | 4 cut(s) | 252, 415, 582, 726 |
| DdeI | CTNAG | 8 cut(s) | 35, 160, 422, 428, 577, 759, 855, 1131 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1074 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1074 |
| Eam1104I | CTCTTC | 4 cut(s) | 760, 959, 1177, 1303 |
| EarI | CTCTTC | 4 cut(s) | 760, 959, 1177, 1303 |
| EciI | GGCGGA | 1 cut(s) | 408 |
| Eco130I | CCWWGG | 4 cut(s) | 196, 268, 958, 1419 |
| Eco47I | GGWCC | 1 cut(s) | 1100 |
| Eco81I | CCTNAGG | 1 cut(s) | 1131 |
| EcoT14I | CCWWGG | 4 cut(s) | 196, 268, 958, 1419 |
| EcoT22I | ATGCAT | 1 cut(s) | 383 |
| ErhI | CCWWGG | 4 cut(s) | 196, 268, 958, 1419 |
| Esp3I | CGTCTC | 1 cut(s) | 690 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1403, 1435 |
| FaqI | GGGAC | 1 cut(s) | 940 |
| FauNDI | CATATG | 1 cut(s) | 937 |
| FbaI | TGATCA | 4 cut(s) | 349, 384, 549, 1320 |
| Fnu4HI | GCNGC | 4 cut(s) | 276, 399, 402, 1050 |
| FokI | GGATG | 3 cut(s) | 420, 559, 1162 |
| Fsp4HI | GCNGC | 4 cut(s) | 276, 399, 402, 1050 |
| FspBI | CTAG | 5 cut(s) | 123, 591, 720, 896, 1058 |
| GlaI | GCGC | 1 cut(s) | 1386 |
| GluI | GCNGC | 4 cut(s) | 276, 399, 402, 1050 |
| GsaI | CCCAGC | 1 cut(s) | 1056 |
| GsuI | CTGGAG | 2 cut(s) | 817, 1027 |
| HaeIII | GGCC | 1 cut(s) | 217 |
| HgaI | GACGC | 1 cut(s) | 665 |
| HhaI | GCGC | 1 cut(s) | 1387 |
| Hin6I | GCGC | 1 cut(s) | 1385 |
| HinP1I | GCGC | 1 cut(s) | 1385 |
| HindIII | AAGCTT | 2 cut(s) | 114, 667 |
| HinfI | GANTC | 8 cut(s) | 130, 149, 192, 264, 504, 674, 910, 919 |
| HphI | GGTGA | 1 cut(s) | 544 |
| Hpy166II | GTNNAC | 4 cut(s) | 1015, 1067, 1358, 1440 |
| Hpy188I | TCNGA | 6 cut(s) | 129, 354, 541, 695, 991, 1320 |
| Hpy188III | TCNNGA | 3 cut(s) | 337, 782, 1162 |
| Hpy8I | GTNNAC | 4 cut(s) | 1015, 1067, 1358, 1440 |
| HpyAV | CCTTC | 1 cut(s) | 607 |
| HpyCH4III | ACNGT | 4 cut(s) | 409, 823, 954, 1255 |
| HpyCH4IV | ACGT | 1 cut(s) | 699 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 214, 1247 |
| HpyF3I | CTNAG | 8 cut(s) | 35, 160, 422, 428, 577, 759, 855, 1131 |
| HpySE526I | ACGT | 1 cut(s) | 699 |
| HspAI | GCGC | 1 cut(s) | 1385 |
| Ksp22I | TGATCA | 4 cut(s) | 349, 384, 549, 1320 |
| LguI | GCTCTTC | 1 cut(s) | 1303 |
| LmnI | GCTCC | 3 cut(s) | 106, 272, 395 |
| Lsp1109I | GCAGC | 4 cut(s) | 287, 388, 410, 1061 |
| LweI | GCATC | 5 cut(s) | 89, 442, 699, 986, 1322 |
| MaeI | CTAG | 5 cut(s) | 123, 591, 720, 896, 1058 |
| MaeII | ACGT | 1 cut(s) | 699 |
| MaeIII | GTNAC | 3 cut(s) | 891, 916, 928 |
| MboII | GAAGA | 9 cut(s) | 111, 592, 634, 716, 777, 976, 1164, 1318, 1320 |
| MflI | RGATCY | 3 cut(s) | 593, 898, 1122 |
| MhlI | GDGCHC | 3 cut(s) | 1360, 1410, 1442 |
| MlyI | GAGTC | 2 cut(s) | 201, 913 |
| MmeI | TCCRAC | 3 cut(s) | 280, 461, 508 |
| Mph1103I | ATGCAT | 1 cut(s) | 383 |
| MseI | TTAA | 2 cut(s) | 948, 1395 |
| MslI | CAYNNNNRTG | 3 cut(s) | 186, 335, 1184 |
| MspA1I | CMGCKG | 2 cut(s) | 401, 1052 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 214, 1247 |
| NdeI | CATATG | 1 cut(s) | 937 |
| NlaIV | GGNNCC | 2 cut(s) | 1101, 1124 |
| NmuCI | GTSAC | 2 cut(s) | 891, 916 |
| NsiI | ATGCAT | 1 cut(s) | 383 |
| NspI | RCATGY | 2 cut(s) | 212, 1280 |
| PagI | TCATGA | 2 cut(s) | 336, 1161 |
| PciI | ACATGT | 1 cut(s) | 1276 |
| PciSI | GCTCTTC | 1 cut(s) | 1303 |
| PfeI | GAWTC | 6 cut(s) | 130, 149, 264, 504, 674, 910 |
| PkrI | GCNGC | 4 cut(s) | 277, 400, 403, 1051 |
| PleI | GAGTC | 2 cut(s) | 200, 913 |
| PpsI | GAGTC | 2 cut(s) | 200, 913 |
| PscI | ACATGT | 1 cut(s) | 1276 |
| PspFI | CCCAGC | 1 cut(s) | 1052 |
| PspN4I | GGNNCC | 2 cut(s) | 1101, 1124 |
| PspPI | GGNCC | 1 cut(s) | 1100 |
| PstNI | CAGNNNCTG | 2 cut(s) | 95, 365 |
| PsuI | RGATCY | 3 cut(s) | 593, 898, 1122 |
| PvuII | CAGCTG | 2 cut(s) | 401, 1052 |
| RsaI | GTAC | 4 cut(s) | 253, 416, 583, 727 |
| RsaNI | GTAC | 4 cut(s) | 252, 415, 582, 726 |
| RseI | CAYNNNNRTG | 3 cut(s) | 186, 335, 1184 |
| SapI | GCTCTTC | 1 cut(s) | 1303 |
| SaqAI | TTAA | 2 cut(s) | 948, 1395 |
| SatI | GCNGC | 4 cut(s) | 276, 399, 402, 1050 |
| Sau96I | GGNCC | 1 cut(s) | 1100 |
| SchI | GAGTC | 2 cut(s) | 201, 913 |
| SduI | GDGCHC | 3 cut(s) | 1360, 1410, 1442 |
| SfaNI | GCATC | 5 cut(s) | 89, 442, 699, 986, 1322 |
| SinI | GGWCC | 1 cut(s) | 1100 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 186, 335, 1184 |
| SmlI | CTYRAG | 1 cut(s) | 443 |
| SmoI | CTYRAG | 1 cut(s) | 443 |
| SsiI | CCGC | 1 cut(s) | 393 |
| SspI | AATATT | 1 cut(s) | 1347 |
| SspMI | CTAG | 5 cut(s) | 123, 591, 720, 896, 1058 |
| StyI | CCWWGG | 4 cut(s) | 196, 268, 958, 1419 |
| TaaI | ACNGT | 4 cut(s) | 409, 823, 954, 1255 |
| TaiI | ACGT | 1 cut(s) | 702 |
| TaqI | TCGA | 1 cut(s) | 374 |
| TatI | WGTACW | 1 cut(s) | 414 |
| TfiI | GAWTC | 6 cut(s) | 130, 149, 264, 504, 674, 910 |
| Tru1I | TTAA | 2 cut(s) | 948, 1395 |
| Tru9I | TTAA | 2 cut(s) | 948, 1395 |
| TscAI | CASTG | 2 cut(s) | 394, 412 |
| TseFI | GTSAC | 2 cut(s) | 891, 916 |
| TseI | GCWGC | 4 cut(s) | 275, 398, 401, 1049 |
| Tsp45I | GTSAC | 2 cut(s) | 891, 916 |
| TspDTI | ATGAA | 3 cut(s) | 924, 1164, 1318 |
| TspRI | CASTG | 2 cut(s) | 394, 412 |
| VneI | GTGCAC | 2 cut(s) | 1356, 1438 |
| VpaK11BI | GGWCC | 1 cut(s) | 1100 |
| XapI | RAATTY | 1 cut(s) | 646 |
| XceI | RCATGY | 2 cut(s) | 212, 1280 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1186 |
| XspI | CTAG | 5 cut(s) | 123, 591, 720, 896, 1058 |
| Zsp2I | ATGCAT | 1 cut(s) | 383 |