Rh1CG154400

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0000623
Physical Location & Seq
Forward (+)
34114476 .. 34116953
2478 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG154400.1

Sequence Viewer

Length: 438 bp
ATGGAAGACTCAGCACAATCCAGAGCAACTCCCACAATTGAATTGCATGGCGATGAATTCTGGCCACTTTCAGGGAAACCTTTCTTTGATATCATACTCACAAAATCCCATGTCAAACCCCTTTACCAAATGGAGATCCCAGCCAAACTGAATCCAGTATTACCCGCTGGTGCTTCAGTTCCTATGGTTCTCAGCTTTGGGGACAAGAGCTGGGAGATGATATATAATGAAACAAAACGTCTTAAATTAGTGGATCGACAATCTTGGAGAGCATTTGTCGATGACAACAGTCTGAAGGCAGGAGATGGATGTATTTTCGAACTAACGGGGTGTGACAGTACAAAAGTAGTATTCAAGGTCCAAATCCTCAGAGGTGACATCCCAGCTGAGCTAGTAGAGAAGTACTCCGGCGAGACAGAGAAACCCATTATTATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

145

Amino Acids

16.27

Weight (kDa)

5.21

Isoelectric Point (pI)

31.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 29 - 120 4.3e-09 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000623)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g35581 FvH4_6g35581 FvH4_6g35582 FvH4_7g07710 FvH4_7g07720
malus_domestica MD02G1233300.v1.1 MD07G1080300.v1.1
prunus_persica Prupe.2G100300_v2.0.a1 Prupe.2G100300_v2.0.a1 Prupe.2G100300_v2.0.a1 Prupe.2G100300_v2.0.a1 Prupe.2G104300_v2.0.a1 Prupe.2G104700_v2.0.a1 Prupe.2G104700_v2.0.a1 Prupe.2G104700_v2.0.a1
pyrus_communis pycom02g20150 pycom07g06390
rosa_chinensis RchiOBHm_Chr1g0342371 RchiOBHm_Chr1g0342381 RchiOBHm_Chr1g0342401 RchiOBHm_Chr1g0342491 RchiOBHm_Chr1g0342511 RchiOBHm_Chr2g0147401 RchiOBHm_Chr2g0147411
rosa_laevigata RLG00000020253 RLG00000020259 RLG00000029157 RLG00000029158
rosa_multiflora Rmu_sc0000979.1_g000010 Rmu_sc0001159.1_g000034 Rmu_sc0001159.1_g000035 Rmu_sc0005388.1_g000001 Rmu_sc0009535.1_g000001 Rmu_sc0009535.1_g000003 Rmu_sc0011976.1_g000004 Rmu_sc0011976.1_g000005 Rmu_sc0027133.1_g000002
rosa_roxburghii Rroxscaffold_2G00099550 Rroxscaffold_2G00099560 Rroxscaffold_4G00296180 Rroxscaffold_4G00312990 Rroxscaffold_4G00313010 Rroxscaffold_4G00313030 Rroxscaffold_4G00313060
rosa_rugosa Rorug01G0150600.1 Rorug01G0150700.1 Rorug01G0150800.1 Rorug01G0150900.1 Rorug01G0151000.1 Rorug01G0151100.1 Rorug01G0151200.1 Rorug01G0151300.1 Rorug01G0151400.1 Rorug02G0399500 Rorug02G0399600 Rorug02G0399700 Rorug02G0399800 Rorug02G0399800 Rorug02G0399900
rosa_samantha Rh1AG165800 Rh1AG165900 Rh1BG132300 Rh1BG132400 Rh1CG154400 Rh1CG154500 Rh1DG167200 Rh1DG167300 Rh2AG456600 Rh2AG456700 Rh2BG469100 Rh2CG444100 Rh2CG444200 Rh2DG478400 Rh2DG478500
rosa_wichuraiana Rw1G013810 Rw1G013820 Rw1G013860 Rw2G037370 Rw2G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 165
AclWI GGATC 2 cut(s) 130, 261
AcoI YGGCCR 1 cut(s) 62
AcsI RAATTY 1 cut(s) 56
AcuI CTGAAG 2 cut(s) 159, 314
AfaI GTAC 2 cut(s) 340, 404
AfiI CCNNNNNNNGG 1 cut(s) 71
AgsI TTSAA 2 cut(s) 41, 355
AluBI AGCT 4 cut(s) 195, 210, 386, 391
AluI AGCT 4 cut(s) 195, 210, 386, 391
Alw26I GTCTC 1 cut(s) 407
AlwI GGATC 2 cut(s) 130, 261
AoxI GGCC 1 cut(s) 62
ApoI RAATTY 1 cut(s) 56
Asp700I GAANNNNTTC 1 cut(s) 80
AspS9I GGNCC 1 cut(s) 358
AsuHPI GGTGA 1 cut(s) 386
AsuII TTCGAA 1 cut(s) 318
AvaII GGWCC 1 cut(s) 358
BalI TGGCCA 1 cut(s) 64
BbsI GAAGAC 1 cut(s) 12
BccI CCATC 1 cut(s) 299
BcoDI GTCTC 1 cut(s) 407
BfaI CTAG 1 cut(s) 392
BlpI GCTNAGC 1 cut(s) 387
BmcAI AGTACT 1 cut(s) 404
Bme18I GGWCC 1 cut(s) 358
BmgT120I GGNCC 1 cut(s) 358
BoxI GACNNNNGTC 1 cut(s) 288
BpiI GAAGAC 1 cut(s) 12
BplI GAGNNNNNCTC 2 cut(s) 389, 421
Bpu1102I GCTNAGC 1 cut(s) 387
Bpu14I TTCGAA 1 cut(s) 318
Bsc4I CCNNNNNNNGG 1 cut(s) 71
Bse1I ACTGG 1 cut(s) 155
BseGI GGATG 2 cut(s) 314, 378
BseLI CCNNNNNNNGG 1 cut(s) 71
BseMII CTCAG 4 cut(s) 24, 205, 378, 382
BseNI ACTGG 1 cut(s) 155
BseYI CCCAGC 3 cut(s) 139, 210, 382
BshFI GGCC 1 cut(s) 64
BsiSI CCGG 1 cut(s) 408
BslFI GGGAC 1 cut(s) 215
BslI CCNNNNNNNGG 1 cut(s) 71
BsmAI GTCTC 1 cut(s) 407
BsmFI GGGAC 1 cut(s) 215
BsnI GGCC 1 cut(s) 64
Bsp119I TTCGAA 1 cut(s) 318
Bsp143I GATC 2 cut(s) 135, 253
Bsp1720I GCTNAGC 1 cut(s) 387
BspACI CCGC 1 cut(s) 165
BspANI GGCC 1 cut(s) 64
BspCNI CTCAG 4 cut(s) 23, 204, 379, 381
BspPI GGATC 2 cut(s) 130, 261
BspT104I TTCGAA 1 cut(s) 318
BsrI ACTGG 1 cut(s) 155
BssMI GATC 2 cut(s) 135, 253
Bst4CI ACNGT 2 cut(s) 290, 338
BstBI TTCGAA 1 cut(s) 318
BstDEI CTNAG 4 cut(s) 10, 191, 368, 387
BstF5I GGATG 2 cut(s) 314, 378
BstKTI GATC 2 cut(s) 138, 256
BstMAI GTCTC 1 cut(s) 407
BstMBI GATC 2 cut(s) 135, 253
BstPAI GACNNNNGTC 1 cut(s) 288
BstV2I GAAGAC 1 cut(s) 12
BstX2I RGATCY 1 cut(s) 135
BstYI RGATCY 1 cut(s) 135
BsuRI GGCC 1 cut(s) 64
BtgZI GCGATG 1 cut(s) 66
BtsCI GGATG 2 cut(s) 314, 378
Cfr13I GGNCC 1 cut(s) 358
Csp6I GTAC 2 cut(s) 339, 403
CviAII CATG 2 cut(s) 47, 110
CviJI RGCY 6 cut(s) 64, 143, 195, 210, 386, 391
CviKI_1 RGCY 6 cut(s) 64, 143, 195, 210, 386, 391
CviQI GTAC 2 cut(s) 339, 403
DdeI CTNAG 4 cut(s) 10, 191, 368, 387
DpnI GATC 2 cut(s) 137, 255
DpnII GATC 2 cut(s) 135, 253
EaeI YGGCCR 1 cut(s) 62
Eco32I GATATC 1 cut(s) 91
Eco47I GGWCC 1 cut(s) 358
Eco57I CTGAAG 2 cut(s) 159, 314
EcoRI GAATTC 1 cut(s) 56
EcoRV GATATC 1 cut(s) 91
FaeI CATG 2 cut(s) 50, 113
FaiI YATR 8 cut(s) 48, 95, 111, 185, 223, 225, 434, 436
FaqI GGGAC 1 cut(s) 215
FatI CATG 2 cut(s) 46, 109
FauI CCCGC 1 cut(s) 172
FokI GGATG 2 cut(s) 321, 365
FspBI CTAG 1 cut(s) 392
GsaI CCCAGC 3 cut(s) 143, 214, 386
HaeIII GGCC 1 cut(s) 64
HapII CCGG 1 cut(s) 408
Hin1II CATG 2 cut(s) 50, 113
HinfI GANTC 2 cut(s) 8, 151
HpaII CCGG 1 cut(s) 408
HphI GGTGA 1 cut(s) 386
Hpy188I TCNGA 2 cut(s) 294, 371
Hpy188III TCNNGA 1 cut(s) 21
HpyAV CCTTC 1 cut(s) 289
HpyCH4III ACNGT 2 cut(s) 290, 338
HpyCH4IV ACGT 1 cut(s) 238
HpyCH4V TGCA 1 cut(s) 46
HpyF3I CTNAG 4 cut(s) 10, 191, 368, 387
HpySE526I ACGT 1 cut(s) 238
Hsp92II CATG 2 cut(s) 50, 113
Kzo9I GATC 2 cut(s) 135, 253
MaeI CTAG 1 cut(s) 392
MaeII ACGT 1 cut(s) 238
MaeIII GTNAC 2 cut(s) 332, 374
MalI GATC 2 cut(s) 137, 255
MboI GATC 2 cut(s) 135, 253
MboII GAAGA 1 cut(s) 17
MfeI CAATTG 1 cut(s) 36
MflI RGATCY 1 cut(s) 135
MlsI TGGCCA 1 cut(s) 64
MluCI AATT 4 cut(s) 36, 41, 56, 245
MluNI TGGCCA 1 cut(s) 64
MlyI GAGTC 1 cut(s) 2
MnlI CCTC 2 cut(s) 365, 377
Mox20I TGGCCA 1 cut(s) 64
MroXI GAANNNNTTC 1 cut(s) 80
MscI TGGCCA 1 cut(s) 64
MseI TTAA 1 cut(s) 243
MslI CAYNNNNRTG 1 cut(s) 51
Msp20I TGGCCA 1 cut(s) 64
MspA1I CMGCKG 2 cut(s) 167, 386
MspI CCGG 1 cut(s) 408
MunI CAATTG 1 cut(s) 36
NdeII GATC 2 cut(s) 135, 253
NlaIII CATG 2 cut(s) 50, 113
NmuCI GTSAC 2 cut(s) 332, 374
NspV TTCGAA 1 cut(s) 318
PdmI GAANNNNTTC 1 cut(s) 80
PfeI GAWTC 1 cut(s) 151
PleI GAGTC 1 cut(s) 2
PpsI GAGTC 1 cut(s) 2
PshAI GACNNNNGTC 1 cut(s) 288
PspFI CCCAGC 3 cut(s) 139, 210, 382
PspPI GGNCC 1 cut(s) 358
PsuI RGATCY 1 cut(s) 135
PvuII CAGCTG 1 cut(s) 386
RsaI GTAC 2 cut(s) 340, 404
RsaNI GTAC 2 cut(s) 339, 403
RseI CAYNNNNRTG 1 cut(s) 51
SaqAI TTAA 1 cut(s) 243
Sau3AI GATC 2 cut(s) 135, 253
Sau96I GGNCC 1 cut(s) 358
ScaI AGTACT 1 cut(s) 404
SchI GAGTC 1 cut(s) 2
SetI ASST 8 cut(s) 82, 197, 212, 241, 360, 376, 388, 393
SfuI TTCGAA 1 cut(s) 318
SinI GGWCC 1 cut(s) 358
SmiMI CAYNNNNRTG 1 cut(s) 51
Sse9I AATT 4 cut(s) 36, 41, 56, 245
SsiI CCGC 1 cut(s) 165
SspMI CTAG 1 cut(s) 392
TaaI ACNGT 2 cut(s) 290, 338
TaiI ACGT 1 cut(s) 241
TaqI TCGA 3 cut(s) 256, 279, 318
TasI AATT 4 cut(s) 36, 41, 56, 245
TatI WGTACW 2 cut(s) 338, 402
TfiI GAWTC 1 cut(s) 151
Tru1I TTAA 1 cut(s) 243
Tru9I TTAA 1 cut(s) 243
TseFI GTSAC 2 cut(s) 332, 374
Tsp45I GTSAC 2 cut(s) 332, 374
TspDTI ATGAA 2 cut(s) 69, 243
VpaK11BI GGWCC 1 cut(s) 358
XapI RAATTY 1 cut(s) 56
XmnI GAANNNNTTC 1 cut(s) 80
XspI CTAG 1 cut(s) 392
ZrmI AGTACT 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.