Rw1G013820

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000623
Physical Location & Seq
Forward (+)
30816371 .. 30817047
677 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G013820.1

Sequence Viewer

Length: 429 bp
ATGGAAGACTCGGCACAATTCAGAGCAACGCCCACAATTGAATTGCATGGCGATGAATTCTGGCCACTTTCAGGGAAACCTTTCTTTGATATCATACTAACAAAATCCCATGTCAAACCCATTTATGAAATGGAGATCCCAGCCAAACTTGATCCTGTATTACCCACTGGTGCTTCAGTCCCCATGGTTCTCAGCTTTGGGGACAAGAGCTGGGAGATGACATATAATGAAATAAAACTAGTGGATCGACACTCGTGGAGAGCATTCGTCGATGACAACAGTCTGAAGGCTGGAGATGGATGTATTTTTGAACTAACGAGGTGTGACAGTACAAAAGTAGTATTCAAGGTCCAAATCCTCAGAGGTGACATCCCAGCTGAGCTAGTAGAGAAGTACCCCGGTGAGGCAGAGAAACCCATTATTATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

142

Amino Acids

16.06

Weight (kDa)

4.94

Isoelectric Point (pI)

20.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 64 - 117 1.3e-07 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000623)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g35581 FvH4_6g35581 FvH4_6g35582 FvH4_7g07710 FvH4_7g07720
malus_domestica MD02G1233300.v1.1 MD07G1080300.v1.1
prunus_persica Prupe.2G100300_v2.0.a1 Prupe.2G100300_v2.0.a1 Prupe.2G100300_v2.0.a1 Prupe.2G100300_v2.0.a1 Prupe.2G104300_v2.0.a1 Prupe.2G104700_v2.0.a1 Prupe.2G104700_v2.0.a1 Prupe.2G104700_v2.0.a1
pyrus_communis pycom02g20150 pycom07g06390
rosa_chinensis RchiOBHm_Chr1g0342371 RchiOBHm_Chr1g0342381 RchiOBHm_Chr1g0342401 RchiOBHm_Chr1g0342491 RchiOBHm_Chr1g0342511 RchiOBHm_Chr2g0147401 RchiOBHm_Chr2g0147411
rosa_laevigata RLG00000020253 RLG00000020259 RLG00000029157 RLG00000029158
rosa_multiflora Rmu_sc0000979.1_g000010 Rmu_sc0001159.1_g000034 Rmu_sc0001159.1_g000035 Rmu_sc0005388.1_g000001 Rmu_sc0009535.1_g000001 Rmu_sc0009535.1_g000003 Rmu_sc0011976.1_g000004 Rmu_sc0011976.1_g000005 Rmu_sc0027133.1_g000002
rosa_roxburghii Rroxscaffold_2G00099550 Rroxscaffold_2G00099560 Rroxscaffold_4G00296180 Rroxscaffold_4G00312990 Rroxscaffold_4G00313010 Rroxscaffold_4G00313030 Rroxscaffold_4G00313060
rosa_rugosa Rorug01G0150600.1 Rorug01G0150700.1 Rorug01G0150800.1 Rorug01G0150900.1 Rorug01G0151000.1 Rorug01G0151100.1 Rorug01G0151200.1 Rorug01G0151300.1 Rorug01G0151400.1 Rorug02G0399500 Rorug02G0399600 Rorug02G0399700 Rorug02G0399800 Rorug02G0399800 Rorug02G0399900
rosa_samantha Rh1AG165800 Rh1AG165900 Rh1BG132300 Rh1BG132400 Rh1CG154400 Rh1CG154500 Rh1DG167200 Rh1DG167300 Rh2AG456600 Rh2AG456700 Rh2BG469100 Rh2CG444100 Rh2CG444200 Rh2DG478400 Rh2DG478500
rosa_wichuraiana Rw1G013810 Rw1G013820 Rw1G013860 Rw2G037370 Rw2G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 130, 146, 252
AcoI YGGCCR 1 cut(s) 62
AcsI RAATTY 1 cut(s) 56
AcuI CTGAAG 2 cut(s) 159, 305
AfaI GTAC 2 cut(s) 331, 395
AfiI CCNNNNNNNGG 2 cut(s) 71, 403
AgsI TTSAA 3 cut(s) 41, 311, 346
AhlI ACTAGT 1 cut(s) 238
AluBI AGCT 4 cut(s) 195, 210, 377, 382
AluI AGCT 4 cut(s) 195, 210, 377, 382
AlwI GGATC 3 cut(s) 130, 146, 252
AoxI GGCC 1 cut(s) 62
ApoI RAATTY 1 cut(s) 56
Asp700I GAANNNNTTC 1 cut(s) 80
AspS9I GGNCC 1 cut(s) 349
AsuC2I CCSGG 1 cut(s) 399
AsuHPI GGTGA 2 cut(s) 377, 413
AvaII GGWCC 1 cut(s) 349
BalI TGGCCA 1 cut(s) 64
BauI CACGAG 1 cut(s) 253
BbsI GAAGAC 1 cut(s) 12
BccI CCATC 1 cut(s) 290
BcnI CCSGG 1 cut(s) 399
BcuI ACTAGT 1 cut(s) 238
BfaI CTAG 2 cut(s) 239, 383
BlpI GCTNAGC 1 cut(s) 378
Bme1390I CCNGG 1 cut(s) 399
Bme18I GGWCC 1 cut(s) 349
BmgT120I GGNCC 1 cut(s) 349
BmrFI CCNGG 1 cut(s) 399
BoxI GACNNNNGTC 1 cut(s) 279
BpiI GAAGAC 1 cut(s) 12
BpmI CTGGAG 1 cut(s) 312
Bpu1102I GCTNAGC 1 cut(s) 378
BpuMI CCSGG 1 cut(s) 399
BsaJI CCNNGG 2 cut(s) 183, 397
Bsc4I CCNNNNNNNGG 2 cut(s) 71, 403
Bse1I ACTGG 1 cut(s) 172
BseDI CCNNGG 2 cut(s) 183, 397
BseGI GGATG 2 cut(s) 305, 369
BseLI CCNNNNNNNGG 2 cut(s) 71, 403
BseMII CTCAG 3 cut(s) 205, 369, 373
BseNI ACTGG 1 cut(s) 172
BseYI CCCAGC 3 cut(s) 139, 210, 373
BshFI GGCC 1 cut(s) 64
BsiSI CCGG 1 cut(s) 399
BslFI GGGAC 2 cut(s) 164, 215
BslI CCNNNNNNNGG 2 cut(s) 71, 403
BsmFI GGGAC 2 cut(s) 164, 215
BsmI GAATGC 1 cut(s) 263
BsnI GGCC 1 cut(s) 64
Bsp143I GATC 3 cut(s) 135, 151, 244
Bsp1720I GCTNAGC 1 cut(s) 378
Bsp19I CCATGG 1 cut(s) 183
BspANI GGCC 1 cut(s) 64
BspCNI CTCAG 3 cut(s) 204, 370, 372
BspPI GGATC 3 cut(s) 130, 146, 252
BsrI ACTGG 1 cut(s) 172
BssECI CCNNGG 2 cut(s) 183, 397
BssMI GATC 3 cut(s) 135, 151, 244
BssSI CACGAG 1 cut(s) 253
BssT1I CCWWGG 1 cut(s) 183
Bst2BI CACGAG 1 cut(s) 253
Bst4CI ACNGT 2 cut(s) 281, 329
BstDEI CTNAG 3 cut(s) 191, 359, 378
BstDSI CCRYGG 1 cut(s) 183
BstF5I GGATG 2 cut(s) 305, 369
BstKTI GATC 3 cut(s) 138, 154, 247
BstMBI GATC 3 cut(s) 135, 151, 244
BstPAI GACNNNNGTC 1 cut(s) 279
BstSCI CCNGG 1 cut(s) 397
BstV2I GAAGAC 1 cut(s) 12
BstX2I RGATCY 1 cut(s) 135
BstYI RGATCY 1 cut(s) 135
BsuRI GGCC 1 cut(s) 64
BtgI CCRYGG 1 cut(s) 183
BtgZI GCGATG 1 cut(s) 66
BtsCI GGATG 2 cut(s) 305, 369
BtsIMutI CAGTG 1 cut(s) 165
Cfr13I GGNCC 1 cut(s) 349
Csp6I GTAC 2 cut(s) 330, 394
CviAII CATG 3 cut(s) 47, 110, 184
CviJI RGCY 7 cut(s) 64, 143, 195, 210, 290, 377, 382
CviKI_1 RGCY 7 cut(s) 64, 143, 195, 210, 290, 377, 382
CviQI GTAC 2 cut(s) 330, 394
DdeI CTNAG 3 cut(s) 191, 359, 378
DpnI GATC 3 cut(s) 137, 153, 246
DpnII GATC 3 cut(s) 135, 151, 244
EaeI YGGCCR 1 cut(s) 62
Eco130I CCWWGG 1 cut(s) 183
Eco32I GATATC 1 cut(s) 91
Eco47I GGWCC 1 cut(s) 349
Eco57I CTGAAG 2 cut(s) 159, 305
EcoRI GAATTC 1 cut(s) 56
EcoRV GATATC 1 cut(s) 91
EcoT14I CCWWGG 1 cut(s) 183
ErhI CCWWGG 1 cut(s) 183
FaeI CATG 3 cut(s) 50, 113, 187
FaiI YATR 9 cut(s) 48, 95, 111, 126, 185, 223, 225, 425, 427
FaqI GGGAC 2 cut(s) 164, 215
FatI CATG 3 cut(s) 46, 109, 183
FokI GGATG 2 cut(s) 312, 356
FspBI CTAG 2 cut(s) 239, 383
GsaI CCCAGC 3 cut(s) 143, 214, 377
GsuI CTGGAG 1 cut(s) 312
HaeIII GGCC 1 cut(s) 64
HapII CCGG 1 cut(s) 399
Hin1II CATG 3 cut(s) 50, 113, 187
HinfI GANTC 1 cut(s) 8
HpaII CCGG 1 cut(s) 399
HphI GGTGA 2 cut(s) 377, 413
Hpy188I TCNGA 3 cut(s) 23, 285, 362
Hpy99I CGWCG 1 cut(s) 272
HpyAV CCTTC 1 cut(s) 280
HpyCH4III ACNGT 2 cut(s) 281, 329
HpyCH4V TGCA 1 cut(s) 46
HpyF3I CTNAG 3 cut(s) 191, 359, 378
Hsp92II CATG 3 cut(s) 50, 113, 187
Kzo9I GATC 3 cut(s) 135, 151, 244
LpnPI CCDG 9 cut(s) 46, 57, 153, 153, 168, 196, 276, 387, 412
MaeI CTAG 2 cut(s) 239, 383
MaeIII GTNAC 2 cut(s) 323, 365
MalI GATC 3 cut(s) 137, 153, 246
MboI GATC 3 cut(s) 135, 151, 244
MboII GAAGA 1 cut(s) 17
MfeI CAATTG 1 cut(s) 36
MflI RGATCY 1 cut(s) 135
MlsI TGGCCA 1 cut(s) 64
MluCI AATT 4 cut(s) 17, 36, 41, 56
MluNI TGGCCA 1 cut(s) 64
MlyI GAGTC 1 cut(s) 2
MnlI CCTC 4 cut(s) 312, 356, 368, 397
Mox20I TGGCCA 1 cut(s) 64
MroXI GAANNNNTTC 1 cut(s) 80
MscI TGGCCA 1 cut(s) 64
MslI CAYNNNNRTG 1 cut(s) 51
Msp20I TGGCCA 1 cut(s) 64
MspA1I CMGCKG 1 cut(s) 377
MspI CCGG 1 cut(s) 399
MspR9I CCNGG 1 cut(s) 399
MunI CAATTG 1 cut(s) 36
Mva1269I GAATGC 1 cut(s) 263
NciI CCSGG 1 cut(s) 399
NcoI CCATGG 1 cut(s) 183
NdeII GATC 3 cut(s) 135, 151, 244
NlaIII CATG 3 cut(s) 50, 113, 187
NmuCI GTSAC 2 cut(s) 323, 365
PctI GAATGC 1 cut(s) 263
PdmI GAANNNNTTC 1 cut(s) 80
PleI GAGTC 1 cut(s) 2
PpsI GAGTC 1 cut(s) 2
PshAI GACNNNNGTC 1 cut(s) 279
PspFI CCCAGC 3 cut(s) 139, 210, 373
PspPI GGNCC 1 cut(s) 349
PsuI RGATCY 1 cut(s) 135
PvuII CAGCTG 1 cut(s) 377
RsaI GTAC 2 cut(s) 331, 395
RsaNI GTAC 2 cut(s) 330, 394
RseI CAYNNNNRTG 1 cut(s) 51
Sau3AI GATC 3 cut(s) 135, 151, 244
Sau96I GGNCC 1 cut(s) 349
SchI GAGTC 1 cut(s) 2
ScrFI CCNGG 1 cut(s) 399
SetI ASST 8 cut(s) 82, 197, 212, 323, 351, 367, 379, 384
SinI GGWCC 1 cut(s) 349
SmiMI CAYNNNNRTG 1 cut(s) 51
SpeI ACTAGT 1 cut(s) 238
Sse9I AATT 4 cut(s) 17, 36, 41, 56
SspMI CTAG 2 cut(s) 239, 383
StyD4I CCNGG 1 cut(s) 397
StyI CCWWGG 1 cut(s) 183
TaaI ACNGT 2 cut(s) 281, 329
TaqI TCGA 2 cut(s) 247, 270
TasI AATT 4 cut(s) 17, 36, 41, 56
TatI WGTACW 1 cut(s) 329
TscAI CASTG 1 cut(s) 172
TseFI GTSAC 2 cut(s) 323, 365
Tsp45I GTSAC 2 cut(s) 323, 365
TspDTI ATGAA 3 cut(s) 69, 141, 243
TspRI CASTG 1 cut(s) 172
VpaK11BI GGWCC 1 cut(s) 349
XapI RAATTY 1 cut(s) 56
XcmI CCANNNNNNNNNTGG 1 cut(s) 127
XmnI GAANNNNTTC 1 cut(s) 80
XspI CTAG 2 cut(s) 239, 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.