RLG00000029157

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000623
Physical Location & Seq
Reverse (-)
33823563 .. 33824847
1285 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029157

Sequence Viewer

Length: 588 bp
ATGCAGTCTCGTGAACTTGTTGCAGAATCAGAATCATGGTACGTTATAAAGTTTGAGAGGGAGGTCCGCACAATCATGGTTATAAAGTTTGAGTGGAAGATTTTGAAAGGTAATTTCTTGGCTGATTATCCTGAAATGGGAGATCCTGCAAGCCAATCCTCCTCATCAGATCCCACGATTGAATTGCGTGGCGATGAATTCTGGCCACTTTCAGGGAAACCTTTCTTTGACATCATCCTCGCAAAATCACATGTCAACCCCAAATGTCAAATGTCGATCCCATCCAAACTACATCCACTACTACTGTCCTGTTCAGTCCCTGTGGTTCTCACTTTTGGGGGCAAGACTTGGGAAATGACATGCAATACAGCAAACAATGCTCAGAAGTTCTTTGATAGACCGTCGTGGAAAGCATTCGTTGACGACAACAATCTGAAGGTCGGAGATGGGTGTGTGTTCGAACTTATGGAGTGCAGCAGCACAAAGCTGGTATTTAGAGTCCAAATTCTCAGAGGTGATATCCCAGCTCAACTTCTAGACAAGGTCAGCGGTGAAAATGCAGAGAAACCCCTTGTGATTCTTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

196

Amino Acids

22.04

Weight (kDa)

5.33

Isoelectric Point (pI)

34.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 76 - 167 1.2e-12 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000623)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g35581 FvH4_6g35581 FvH4_6g35582 FvH4_7g07710 FvH4_7g07720
malus_domestica MD02G1233300.v1.1 MD07G1080300.v1.1
prunus_persica Prupe.2G100300_v2.0.a1 Prupe.2G100300_v2.0.a1 Prupe.2G100300_v2.0.a1 Prupe.2G100300_v2.0.a1 Prupe.2G104300_v2.0.a1 Prupe.2G104700_v2.0.a1 Prupe.2G104700_v2.0.a1 Prupe.2G104700_v2.0.a1
pyrus_communis pycom02g20150 pycom07g06390
rosa_chinensis RchiOBHm_Chr1g0342371 RchiOBHm_Chr1g0342381 RchiOBHm_Chr1g0342401 RchiOBHm_Chr1g0342491 RchiOBHm_Chr1g0342511 RchiOBHm_Chr2g0147401 RchiOBHm_Chr2g0147411
rosa_laevigata RLG00000020253 RLG00000020259 RLG00000029157 RLG00000029158
rosa_multiflora Rmu_sc0000979.1_g000010 Rmu_sc0001159.1_g000034 Rmu_sc0001159.1_g000035 Rmu_sc0005388.1_g000001 Rmu_sc0009535.1_g000001 Rmu_sc0009535.1_g000003 Rmu_sc0011976.1_g000004 Rmu_sc0011976.1_g000005 Rmu_sc0027133.1_g000002
rosa_roxburghii Rroxscaffold_2G00099550 Rroxscaffold_2G00099560 Rroxscaffold_4G00296180 Rroxscaffold_4G00312990 Rroxscaffold_4G00313010 Rroxscaffold_4G00313030 Rroxscaffold_4G00313060
rosa_rugosa Rorug01G0150600.1 Rorug01G0150700.1 Rorug01G0150800.1 Rorug01G0150900.1 Rorug01G0151000.1 Rorug01G0151100.1 Rorug01G0151200.1 Rorug01G0151300.1 Rorug01G0151400.1 Rorug02G0399500 Rorug02G0399600 Rorug02G0399700 Rorug02G0399800 Rorug02G0399800 Rorug02G0399900
rosa_samantha Rh1AG165800 Rh1AG165900 Rh1BG132300 Rh1BG132400 Rh1CG154400 Rh1CG154500 Rh1DG167200 Rh1DG167300 Rh2AG456600 Rh2AG456700 Rh2BG469100 Rh2CG444100 Rh2CG444200 Rh2DG478400 Rh2DG478500
rosa_wichuraiana Rw1G013810 Rw1G013820 Rw1G013860 Rw2G037370 Rw2G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 47, 83
AciI CCGC 2 cut(s) 67, 549
AclWI GGATC 3 cut(s) 137, 164, 271
AcoI YGGCCR 1 cut(s) 203
AcsI RAATTY 2 cut(s) 197, 504
AcuI CTGAAG 1 cut(s) 455
AfaI GTAC 1 cut(s) 41
AfiI CCNNNNNNNGG 2 cut(s) 137, 212
AflIII ACRYGT 1 cut(s) 250
AgsI TTSAA 2 cut(s) 106, 182
AluBI AGCT 2 cut(s) 487, 527
AluI AGCT 2 cut(s) 487, 527
Alw26I GTCTC 1 cut(s) 12
AlwI GGATC 3 cut(s) 137, 164, 271
AlwNI CAGNNNCTG 1 cut(s) 320
AoxI GGCC 1 cut(s) 203
ApeKI GCWGC 2 cut(s) 474, 477
ApoI RAATTY 2 cut(s) 197, 504
Asp700I GAANNNNTTC 2 cut(s) 221, 413
AspS9I GGNCC 1 cut(s) 64
AsuHPI GGTGA 2 cut(s) 527, 563
AsuII TTCGAA 1 cut(s) 459
AvaII GGWCC 1 cut(s) 64
BalI TGGCCA 1 cut(s) 205
BauI CACGAG 1 cut(s) 9
BbvI GCAGC 2 cut(s) 486, 489
BccI CCATC 2 cut(s) 289, 440
BcgI CGANNNNNNTGC 2 cut(s) 166, 200
BcoDI GTCTC 1 cut(s) 12
BfaI CTAG 1 cut(s) 536
BisI GCNGC 2 cut(s) 475, 478
BlsI GCNGC 2 cut(s) 476, 479
Bme18I GGWCC 1 cut(s) 64
BmgT120I GGNCC 1 cut(s) 64
Bpu14I TTCGAA 1 cut(s) 459
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 212
BseGI GGATG 3 cut(s) 234, 281, 292
BseLI CCNNNNNNNGG 2 cut(s) 137, 212
BseMII CTCAG 2 cut(s) 395, 523
BseRI GAGGAG 1 cut(s) 151
BseXI GCAGC 2 cut(s) 486, 489
BseYI CCCAGC 1 cut(s) 523
BsgI GTGCAG 1 cut(s) 493
BshFI GGCC 1 cut(s) 205
BslFI GGGAC 1 cut(s) 302
BslI CCNNNNNNNGG 2 cut(s) 137, 212
BsmAI GTCTC 1 cut(s) 12
BsmFI GGGAC 1 cut(s) 302
BsmI GAATGC 1 cut(s) 413
BsnI GGCC 1 cut(s) 205
Bsp119I TTCGAA 1 cut(s) 459
Bsp143I GATC 3 cut(s) 142, 169, 276
BspACI CCGC 2 cut(s) 67, 549
BspANI GGCC 1 cut(s) 205
BspCNI CTCAG 2 cut(s) 394, 522
BspPI GGATC 3 cut(s) 137, 164, 271
BspT104I TTCGAA 1 cut(s) 459
BssMI GATC 3 cut(s) 142, 169, 276
BssSI CACGAG 1 cut(s) 9
Bst2BI CACGAG 1 cut(s) 9
Bst4CI ACNGT 2 cut(s) 306, 402
BstAPI GCANNNNNTGC 1 cut(s) 377
BstBI TTCGAA 1 cut(s) 459
BstC8I GCNNGC 1 cut(s) 151
BstDEI CTNAG 2 cut(s) 381, 509
BstF5I GGATG 3 cut(s) 234, 281, 292
BstKTI GATC 3 cut(s) 145, 172, 279
BstMAI GTCTC 1 cut(s) 12
BstMBI GATC 3 cut(s) 142, 169, 276
BstMWI GCNNNNNNNGC 1 cut(s) 377
BstNSI RCATGY 2 cut(s) 254, 363
BstV1I GCAGC 2 cut(s) 486, 489
BstX2I RGATCY 2 cut(s) 142, 169
BstYI RGATCY 2 cut(s) 142, 169
BsuRI GGCC 1 cut(s) 205
BtgZI GCGATG 1 cut(s) 207
BtsCI GGATG 3 cut(s) 234, 281, 292
Cac8I GCNNGC 1 cut(s) 151
CaiI CAGNNNCTG 1 cut(s) 320
Cfr13I GGNCC 1 cut(s) 64
Csp6I GTAC 1 cut(s) 40
CviAII CATG 4 cut(s) 36, 76, 251, 360
CviJI RGCY 5 cut(s) 122, 153, 205, 487, 527
CviKI_1 RGCY 5 cut(s) 122, 153, 205, 487, 527
CviQI GTAC 1 cut(s) 40
DdeI CTNAG 2 cut(s) 381, 509
DpnI GATC 3 cut(s) 144, 171, 278
DpnII GATC 3 cut(s) 142, 169, 276
EaeI YGGCCR 1 cut(s) 203
Eco32I GATATC 1 cut(s) 520
Eco47I GGWCC 1 cut(s) 64
Eco57I CTGAAG 1 cut(s) 455
EcoRI GAATTC 1 cut(s) 197
EcoRV GATATC 1 cut(s) 520
FaeI CATG 4 cut(s) 39, 79, 254, 363
FaiI YATR 7 cut(s) 37, 47, 77, 83, 252, 361, 467
FaqI GGGAC 1 cut(s) 302
FatI CATG 4 cut(s) 35, 75, 250, 359
Fnu4HI GCNGC 2 cut(s) 475, 478
FokI GGATG 3 cut(s) 221, 268, 279
Fsp4HI GCNGC 2 cut(s) 475, 478
FspBI CTAG 1 cut(s) 536
GluI GCNGC 2 cut(s) 475, 478
GsaI CCCAGC 1 cut(s) 527
HaeIII GGCC 1 cut(s) 205
Hin1II CATG 4 cut(s) 39, 79, 254, 363
HincII GTYRAC 2 cut(s) 256, 421
HindII GTYRAC 2 cut(s) 256, 421
HinfI GANTC 4 cut(s) 26, 32, 498, 577
HphI GGTGA 2 cut(s) 527, 563
Hpy166II GTNNAC 3 cut(s) 14, 256, 421
Hpy188I TCNGA 6 cut(s) 31, 169, 384, 435, 443, 512
Hpy188III TCNNGA 4 cut(s) 11, 131, 536, 581
Hpy8I GTNNAC 3 cut(s) 14, 256, 421
Hpy99I CGWCG 1 cut(s) 406
HpyAV CCTTC 1 cut(s) 430
HpyCH4III ACNGT 2 cut(s) 306, 402
HpyCH4IV ACGT 1 cut(s) 42
HpyCH4V TGCA 6 cut(s) 4, 23, 149, 363, 474, 560
HpyF10VI GCNNNNNNNGC 1 cut(s) 377
HpyF3I CTNAG 2 cut(s) 381, 509
HpySE526I ACGT 1 cut(s) 42
Hsp92II CATG 4 cut(s) 39, 79, 254, 363
Kzo9I GATC 3 cut(s) 142, 169, 276
LpnPI CCDG 8 cut(s) 144, 159, 187, 198, 322, 333, 473, 537
Lsp1109I GCAGC 2 cut(s) 486, 489
MaeI CTAG 1 cut(s) 536
MaeII ACGT 1 cut(s) 42
MalI GATC 3 cut(s) 144, 171, 278
MboI GATC 3 cut(s) 142, 169, 276
MboII GAAGA 1 cut(s) 109
MflI RGATCY 2 cut(s) 142, 169
MlsI TGGCCA 1 cut(s) 205
MluCI AATT 4 cut(s) 112, 182, 197, 504
MluNI TGGCCA 1 cut(s) 205
MlyI GAGTC 1 cut(s) 507
MmeI TCCRAC 1 cut(s) 421
MnlI CCTC 6 cut(s) 51, 55, 169, 172, 248, 506
Mox20I TGGCCA 1 cut(s) 205
MroXI GAANNNNTTC 2 cut(s) 221, 413
MscI TGGCCA 1 cut(s) 205
MslI CAYNNNNRTG 1 cut(s) 74
Msp20I TGGCCA 1 cut(s) 205
MspA1I CMGCKG 1 cut(s) 549
Mva1269I GAATGC 1 cut(s) 413
MwoI GCNNNNNNNGC 1 cut(s) 377
NdeII GATC 3 cut(s) 142, 169, 276
NlaIII CATG 4 cut(s) 39, 79, 254, 363
NspI RCATGY 2 cut(s) 254, 363
NspV TTCGAA 1 cut(s) 459
PciI ACATGT 1 cut(s) 250
PctI GAATGC 1 cut(s) 413
PdmI GAANNNNTTC 2 cut(s) 221, 413
PfeI GAWTC 3 cut(s) 26, 32, 577
PflFI GACNNNGTC 1 cut(s) 542
PkrI GCNGC 2 cut(s) 476, 479
PleI GAGTC 1 cut(s) 506
PpsI GAGTC 1 cut(s) 506
PscI ACATGT 1 cut(s) 250
PsiI TTATAA 2 cut(s) 47, 83
PspFI CCCAGC 1 cut(s) 523
PspPI GGNCC 1 cut(s) 64
PstNI CAGNNNCTG 1 cut(s) 320
PsuI RGATCY 2 cut(s) 142, 169
PsyI GACNNNGTC 1 cut(s) 542
RsaI GTAC 1 cut(s) 41
RsaNI GTAC 1 cut(s) 40
RseI CAYNNNNRTG 1 cut(s) 74
SatI GCNGC 2 cut(s) 475, 478
Sau3AI GATC 3 cut(s) 142, 169, 276
Sau96I GGNCC 1 cut(s) 64
SchI GAGTC 1 cut(s) 507
SetI ASST 9 cut(s) 45, 66, 112, 223, 441, 489, 517, 529, 546
SfuI TTCGAA 1 cut(s) 459
SinI GGWCC 1 cut(s) 64
SmiMI CAYNNNNRTG 1 cut(s) 74
Sse9I AATT 4 cut(s) 112, 182, 197, 504
SsiI CCGC 2 cut(s) 67, 549
SspMI CTAG 1 cut(s) 536
TaaI ACNGT 2 cut(s) 306, 402
TaiI ACGT 1 cut(s) 45
TaqI TCGA 2 cut(s) 275, 459
TasI AATT 4 cut(s) 112, 182, 197, 504
TfiI GAWTC 3 cut(s) 26, 32, 577
TseI GCWGC 2 cut(s) 474, 477
TspDTI ATGAA 1 cut(s) 210
Tth111I GACNNNGTC 1 cut(s) 542
VpaK11BI GGWCC 1 cut(s) 64
XapI RAATTY 2 cut(s) 197, 504
XbaI TCTAGA 1 cut(s) 535
XceI RCATGY 2 cut(s) 254, 363
XmnI GAANNNNTTC 2 cut(s) 221, 413
XspI CTAG 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.