RLG00000020259

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000623
Physical Location & Seq
Reverse (-)
62957984 .. 62962679
4696 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020259

Sequence Viewer

Length: 930 bp
ATGAGCCTTGGAGTACACATCTTTGTTGCAGATCATTTGTATGTGAAGCCAGTTGTAGACCCTGAGATGGCCAAATCTTCATCGAATCCTGTAGTTGAATTGAATGGAGAGGAATTCTGGCCACTTTCTGGGAAACCATACTTTGACGTCATACTCACGAAATCACATGTGAAACCCATTTACCAACTGGGGATCCCAGCCAAACTTGATCCAGTACTTCCCTCTGGTTCAATACATACAGTTCTCACATTTGGGGGTAAGAATTGGGAGATGACCTATAATGGAGAAAAACGTCATAAACAATTCGATCGAAAGTCATGGGGAGCATTTGTCAATGAAAACAATTTGAAGGCTGGAGATGGATTGGTGTTTGAACTCAAGGAGTGCAATAGCACACAAATAGGATTCAGAGTCCAAATCCTGAGAGGTGACATCCCTGACGAACTTCTAGAGAAGGCCAACCAACATTTAGGTGACTTCATGAGGAGAAGTAGTCATATAACCATAGTACACTACAGAGTAGTCATCATCATTATTGTAACATCACCATTATCTCTGGCCAGTGAAATTAGGCAACTTTCACCAGATATTATCCTCACAGTCCCAGTTGGCAAAGCAGAAGATCCTGAAATGTCAGACTCGGCTCAATCCTTATCAGATACAACAATTCAATTGCATGGCGAAAAATTTTGGCCTCTTTCAGGAAAACCTTTCTATGATATAATACTAGCAAAAACACATGTCGAACCGTATTACAAACTGGGGATCCCAGCCAAACTTCATCCAATACTACCATCTTGTTCATTCCCTACGTCAAAATCTCTCAGTGCAGATAAAGATTTTGGCATCATCAATTTTGAGGCACAGAATATACCACACATGATTGATGACTACTTCAGTTGCACTACGAGTTCAAATGCACGTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

310

Amino Acids

34.5

Weight (kDa)

6.17

Isoelectric Point (pI)

45.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 48 - 132 8.9e-09 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000623)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g35581 FvH4_6g35581 FvH4_6g35582 FvH4_7g07710 FvH4_7g07720
malus_domestica MD02G1233300.v1.1 MD07G1080300.v1.1
prunus_persica Prupe.2G100300_v2.0.a1 Prupe.2G100300_v2.0.a1 Prupe.2G100300_v2.0.a1 Prupe.2G100300_v2.0.a1 Prupe.2G104300_v2.0.a1 Prupe.2G104700_v2.0.a1 Prupe.2G104700_v2.0.a1 Prupe.2G104700_v2.0.a1
pyrus_communis pycom02g20150 pycom07g06390
rosa_chinensis RchiOBHm_Chr1g0342371 RchiOBHm_Chr1g0342381 RchiOBHm_Chr1g0342401 RchiOBHm_Chr1g0342491 RchiOBHm_Chr1g0342511 RchiOBHm_Chr2g0147401 RchiOBHm_Chr2g0147411
rosa_laevigata RLG00000020253 RLG00000020259 RLG00000029157 RLG00000029158
rosa_multiflora Rmu_sc0000979.1_g000010 Rmu_sc0001159.1_g000034 Rmu_sc0001159.1_g000035 Rmu_sc0005388.1_g000001 Rmu_sc0009535.1_g000001 Rmu_sc0009535.1_g000003 Rmu_sc0011976.1_g000004 Rmu_sc0011976.1_g000005 Rmu_sc0027133.1_g000002
rosa_roxburghii Rroxscaffold_2G00099550 Rroxscaffold_2G00099560 Rroxscaffold_4G00296180 Rroxscaffold_4G00312990 Rroxscaffold_4G00313010 Rroxscaffold_4G00313030 Rroxscaffold_4G00313060
rosa_rugosa Rorug01G0150600.1 Rorug01G0150700.1 Rorug01G0150800.1 Rorug01G0150900.1 Rorug01G0151000.1 Rorug01G0151100.1 Rorug01G0151200.1 Rorug01G0151300.1 Rorug01G0151400.1 Rorug02G0399500 Rorug02G0399600 Rorug02G0399700 Rorug02G0399800 Rorug02G0399800 Rorug02G0399900
rosa_samantha Rh1AG165800 Rh1AG165900 Rh1BG132300 Rh1BG132400 Rh1CG154400 Rh1CG154500 Rh1DG167200 Rh1DG167300 Rh2AG456600 Rh2AG456700 Rh2BG469100 Rh2CG444100 Rh2CG444200 Rh2DG478400 Rh2DG478500
rosa_wichuraiana Rw1G013810 Rw1G013820 Rw1G013860 Rw2G037370 Rw2G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 150
AccB7I CCANNNNNTGG 1 cut(s) 128
AccI GTMKAC 1 cut(s) 57
AclWI GGATC 6 cut(s) 187, 200, 203, 617, 760, 773
AcoI YGGCCR 3 cut(s) 69, 119, 558
AcsI RAATTY 2 cut(s) 113, 686
AcuI CTGAAG 1 cut(s) 880
AcyI GRCGYC 1 cut(s) 147
AfaI GTAC 3 cut(s) 15, 216, 510
AfiI CCNNNNNNNGG 3 cut(s) 67, 128, 701
AflIII ACRYGT 2 cut(s) 166, 739
AgsI TTSAA 7 cut(s) 98, 103, 231, 349, 374, 671, 915
AlwI GGATC 6 cut(s) 187, 200, 203, 617, 760, 773
AoxI GGCC 5 cut(s) 69, 119, 456, 558, 692
ApoI RAATTY 2 cut(s) 113, 686
AsuHPI GGTGA 4 cut(s) 440, 485, 537, 573
BalI TGGCCA 3 cut(s) 71, 121, 560
BamHI GGATCC 2 cut(s) 192, 765
BccI CCATC 3 cut(s) 61, 353, 802
BfaI CTAG 2 cut(s) 449, 728
BfmI CTRYAG 2 cut(s) 90, 514
BmcAI AGTACT 1 cut(s) 216
BmiI GGNNCC 2 cut(s) 194, 767
BmrI ACTGGG 3 cut(s) 197, 599, 770
BmsI GCATC 1 cut(s) 855
BmuI ACTGGG 3 cut(s) 197, 599, 770
BpmI CTGGAG 1 cut(s) 375
BpuEI CTTGAG 1 cut(s) 362
BsaHI GRCGYC 1 cut(s) 147
BsaJI CCNNGG 1 cut(s) 7
BsaXI ACNNNNNCTCC 4 cut(s) 315, 345, 478, 508
Bsc4I CCNNNNNNNGG 3 cut(s) 67, 128, 701
Bse1I ACTGG 6 cut(s) 50, 192, 212, 561, 605, 765
BseDI CCNNGG 1 cut(s) 7
BseGI GGATG 2 cut(s) 432, 781
BseLI CCNNNNNNNGG 3 cut(s) 67, 128, 701
BseMII CTCAG 3 cut(s) 54, 413, 838
BseNI ACTGG 6 cut(s) 50, 192, 212, 561, 605, 765
BseRI GAGGAG 1 cut(s) 499
BseYI CCCAGC 2 cut(s) 196, 769
BsgI GTGCAG 1 cut(s) 849
Bsh1285I CGRYCG 1 cut(s) 310
BshFI GGCC 5 cut(s) 71, 121, 458, 560, 694
BsiEI CGRYCG 1 cut(s) 310
BslFI GGGAC 1 cut(s) 587
BslI CCNNNNNNNGG 3 cut(s) 67, 128, 701
BsmFI GGGAC 1 cut(s) 587
BsnI GGCC 5 cut(s) 71, 121, 458, 560, 694
Bsp143I GATC 6 cut(s) 31, 192, 208, 307, 622, 765
BspANI GGCC 5 cut(s) 71, 121, 458, 560, 694
BspCNI CTCAG 3 cut(s) 55, 414, 837
BspHI TCATGA 1 cut(s) 480
BspLI GGNNCC 2 cut(s) 194, 767
BspPI GGATC 6 cut(s) 187, 200, 203, 617, 760, 773
BsrI ACTGG 6 cut(s) 50, 192, 212, 561, 605, 765
BssECI CCNNGG 1 cut(s) 7
BssMI GATC 6 cut(s) 31, 192, 208, 307, 622, 765
BssNI GRCGYC 1 cut(s) 147
BssT1I CCWWGG 1 cut(s) 7
Bst4CI ACNGT 3 cut(s) 241, 601, 750
BstACI GRCGYC 1 cut(s) 147
BstDEI CTNAG 3 cut(s) 63, 422, 824
BstENI CCTNNNNNAGG 1 cut(s) 699
BstF5I GGATG 2 cut(s) 432, 781
BstKTI GATC 6 cut(s) 34, 195, 211, 310, 625, 768
BstMBI GATC 6 cut(s) 31, 192, 208, 307, 622, 765
BstMCI CGRYCG 1 cut(s) 310
BstNSI RCATGY 2 cut(s) 170, 743
BstSFI CTRYAG 2 cut(s) 90, 514
BstX2I RGATCY 3 cut(s) 192, 622, 765
BstYI RGATCY 3 cut(s) 192, 622, 765
BsuRI GGCC 5 cut(s) 71, 121, 458, 560, 694
BtsCI GGATG 2 cut(s) 432, 781
BtsIMutI CAGTG 2 cut(s) 568, 832
CciI TCATGA 1 cut(s) 480
Csp6I GTAC 3 cut(s) 14, 215, 509
CviAII CATG 6 cut(s) 167, 318, 481, 677, 740, 880
CviQI GTAC 3 cut(s) 14, 215, 509
DdeI CTNAG 3 cut(s) 63, 422, 824
DpnI GATC 6 cut(s) 33, 194, 210, 309, 624, 767
DpnII GATC 6 cut(s) 31, 192, 208, 307, 622, 765
EaeI YGGCCR 3 cut(s) 69, 119, 558
Eco130I CCWWGG 1 cut(s) 7
Eco57I CTGAAG 1 cut(s) 880
EcoNI CCTNNNNNAGG 1 cut(s) 699
EcoRI GAATTC 1 cut(s) 113
EcoT14I CCWWGG 1 cut(s) 7
ErhI CCWWGG 1 cut(s) 7
FaeI CATG 6 cut(s) 170, 321, 484, 680, 743, 883
FaqI GGGAC 1 cut(s) 587
FatI CATG 6 cut(s) 166, 317, 480, 676, 739, 879
FblI GTMKAC 1 cut(s) 57
FokI GGATG 2 cut(s) 419, 768
FspBI CTAG 2 cut(s) 449, 728
GsaI CCCAGC 2 cut(s) 200, 773
GsuI CTGGAG 1 cut(s) 375
HaeIII GGCC 5 cut(s) 71, 121, 458, 560, 694
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 6 cut(s) 170, 321, 484, 680, 743, 883
HinfI GANTC 4 cut(s) 85, 405, 411, 638
HphI GGTGA 4 cut(s) 440, 485, 537, 573
Hpy166II GTNNAC 3 cut(s) 16, 58, 511
Hpy188I TCNGA 3 cut(s) 410, 637, 658
Hpy188III TCNNGA 6 cut(s) 157, 421, 449, 481, 626, 702
Hpy8I GTNNAC 3 cut(s) 16, 58, 511
HpyAV CCTTC 2 cut(s) 343, 448
HpyCH4III ACNGT 3 cut(s) 241, 601, 750
HpyCH4IV ACGT 4 cut(s) 147, 292, 812, 922
HpyCH4V TGCA 6 cut(s) 29, 387, 676, 830, 903, 920
HpyF3I CTNAG 3 cut(s) 63, 422, 824
HpySE526I ACGT 4 cut(s) 147, 292, 812, 922
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 6 cut(s) 170, 321, 484, 680, 743, 883
Kzo9I GATC 6 cut(s) 31, 192, 208, 307, 622, 765
LmnI GCTCC 1 cut(s) 323
LweI GCATC 1 cut(s) 855
MaeI CTAG 2 cut(s) 449, 728
MaeII ACGT 4 cut(s) 147, 292, 812, 922
MaeIII GTNAC 3 cut(s) 428, 473, 538
MalI GATC 6 cut(s) 33, 194, 210, 309, 624, 767
MboI GATC 6 cut(s) 31, 192, 208, 307, 622, 765
MboII GAAGA 2 cut(s) 69, 632
MfeI CAATTG 1 cut(s) 671
MflI RGATCY 3 cut(s) 192, 622, 765
MlsI TGGCCA 3 cut(s) 71, 121, 560
MluNI TGGCCA 3 cut(s) 71, 121, 560
MlyI GAGTC 2 cut(s) 420, 632
MnlI CCTC 7 cut(s) 103, 232, 419, 477, 605, 705, 853
Mox20I TGGCCA 3 cut(s) 71, 121, 560
MscI TGGCCA 3 cut(s) 71, 121, 560
MslI CAYNNNNRTG 2 cut(s) 39, 471
Msp20I TGGCCA 3 cut(s) 71, 121, 560
MunI CAATTG 1 cut(s) 671
NdeII GATC 6 cut(s) 31, 192, 208, 307, 622, 765
NlaIII CATG 6 cut(s) 170, 321, 484, 680, 743, 883
NlaIV GGNNCC 2 cut(s) 194, 767
NmeAIII GCCGAG 1 cut(s) 620
NmuCI GTSAC 2 cut(s) 428, 473
NspI RCATGY 2 cut(s) 170, 743
PagI TCATGA 1 cut(s) 480
PciI ACATGT 2 cut(s) 166, 739
PfeI GAWTC 2 cut(s) 85, 405
PflMI CCANNNNNTGG 1 cut(s) 128
Ple19I CGATCG 1 cut(s) 310
PleI GAGTC 2 cut(s) 419, 632
PpsI GAGTC 2 cut(s) 419, 632
PscI ACATGT 2 cut(s) 166, 739
PspFI CCCAGC 2 cut(s) 196, 769
PspN4I GGNNCC 2 cut(s) 194, 767
PsuI RGATCY 3 cut(s) 192, 622, 765
PvuI CGATCG 1 cut(s) 310
RsaI GTAC 3 cut(s) 15, 216, 510
RsaNI GTAC 3 cut(s) 14, 215, 509
RseI CAYNNNNRTG 2 cut(s) 39, 471
Sau3AI GATC 6 cut(s) 31, 192, 208, 307, 622, 765
ScaI AGTACT 1 cut(s) 216
SchI GAGTC 2 cut(s) 420, 632
SetI ASST 8 cut(s) 150, 278, 295, 430, 475, 712, 815, 925
SfaNI GCATC 1 cut(s) 855
SfcI CTRYAG 2 cut(s) 90, 514
SmiMI CAYNNNNRTG 2 cut(s) 39, 471
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
SspMI CTAG 2 cut(s) 449, 728
StyI CCWWGG 1 cut(s) 7
TaaI ACNGT 3 cut(s) 241, 601, 750
TaiI ACGT 4 cut(s) 150, 295, 815, 925
TaqI TCGA 4 cut(s) 83, 306, 310, 744
TatI WGTACW 3 cut(s) 13, 214, 508
TfiI GAWTC 2 cut(s) 85, 405
TscAI CASTG 2 cut(s) 568, 832
TseFI GTSAC 2 cut(s) 428, 473
Tsp45I GTSAC 2 cut(s) 428, 473
TspDTI ATGAA 5 cut(s) 69, 351, 469, 770, 792
TspRI CASTG 2 cut(s) 568, 832
Van91I CCANNNNNTGG 1 cut(s) 128
XagI CCTNNNNNAGG 1 cut(s) 699
XapI RAATTY 2 cut(s) 113, 686
XbaI TCTAGA 1 cut(s) 448
XceI RCATGY 2 cut(s) 170, 743
XcmI CCANNNNNNNNNTGG 1 cut(s) 184
XmiI GTMKAC 1 cut(s) 57
XspI CTAG 2 cut(s) 449, 728
ZraI GACGTC 1 cut(s) 148
ZrmI AGTACT 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.