Rroxscaffold_4G00296180

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000623
Physical Location & Seq
Reverse (-)
16237809 .. 16251986
14178 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00296180.1

Sequence Viewer

Length: 1089 bp
ATGGTTTTTGATTTGAATATATTATTTCGTAATATCGCAAGGGTTTTCACCCCGCTTGTTTATCCAATAAAGTTAACATGTTTTATCAAACATGCACGGGATCTTCCATTTGAGTCAACATGTGACCTTGAGAGTCTTCAATCTGTCATTGAAGAGAACAATGAAATCTGCAAGTGGGTACCTACTGCTACGCTGCGCTTCTCCACTCTCAAGCCAACTCTCAACAAGCATCTTCGAGTTATTCGTCAAGTGCCAATTGGCCAAGCAGAAGATACTGAAATGGAAAACTTGGCACAATCCAAAGCAATGCCCACAATTGAATTGCATGGTGATGAATTCTGGCCACTTTCAGGGAAACCTTTCTTTGATATCATACACACAAAATCCCATGTCAAACCCATTTACGAAATGGGATTAGGCGTACGCCTAGTCCATTCTACAAAGAGTCTTCTACAAATGGCTAACAAATCAGGTGTGGGATGGTCGAACGGTGGCAATGGTGTTGGCCTTGGTTTAGGGCGCGAGTCGTGGTGGTGCGAGTTGAGGCGGGTTCGCGAGGTTGATGACGACCGGAGTGTGAAGGGAGGCTCTAGCTGCTCACCGAACCCAACAATCAAGCTTGAGGCCCATATCCCATATGGAGACTCAAGCCTAGCTAGGCAAGGCCTACACCGAAGGTTCGCCATACTCGGCGAAGGCTCGCCGCCGCCGAAAACCAGCCGCCCTCATTTGCGGCGTTCGCATCCTTCATGCCCAAGTCAATCGAAAATCCAACGACGGTCTGCGGCCTTGATACCGGCATTACCACACCAAAAGAAACCCATTAGAGATTCTTCCACCGTCACGCTACCATCCCCGCCAAAGCCACCACGTCCTCCGTCGCAAGACAACCTAGGCGATTCCTACCACGTCCATCCTCATCCTCGTATGTCGAGTCACAAAGACGAAGCAAGCATTGTTTTTGTTATCCTTGTTGGCCCCTTCCTTTGTCTCTTTCTTCCTCCATCAGTGACTCCGAAGAAGACACTAGCAGAGGTCAAGGACTTCGGTGATGAGGGAAATGGAGAATTAGAGATGGTGAGAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

362

Amino Acids

40.38

Weight (kDa)

9.21

Isoelectric Point (pI)

62.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000623)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g35581 FvH4_6g35581 FvH4_6g35582 FvH4_7g07710 FvH4_7g07720
malus_domestica MD02G1233300.v1.1 MD07G1080300.v1.1
prunus_persica Prupe.2G100300_v2.0.a1 Prupe.2G100300_v2.0.a1 Prupe.2G100300_v2.0.a1 Prupe.2G100300_v2.0.a1 Prupe.2G104300_v2.0.a1 Prupe.2G104700_v2.0.a1 Prupe.2G104700_v2.0.a1 Prupe.2G104700_v2.0.a1
pyrus_communis pycom02g20150 pycom07g06390
rosa_chinensis RchiOBHm_Chr1g0342371 RchiOBHm_Chr1g0342381 RchiOBHm_Chr1g0342401 RchiOBHm_Chr1g0342491 RchiOBHm_Chr1g0342511 RchiOBHm_Chr2g0147401 RchiOBHm_Chr2g0147411
rosa_laevigata RLG00000020253 RLG00000020259 RLG00000029157 RLG00000029158
rosa_multiflora Rmu_sc0000979.1_g000010 Rmu_sc0001159.1_g000034 Rmu_sc0001159.1_g000035 Rmu_sc0005388.1_g000001 Rmu_sc0009535.1_g000001 Rmu_sc0009535.1_g000003 Rmu_sc0011976.1_g000004 Rmu_sc0011976.1_g000005 Rmu_sc0027133.1_g000002
rosa_roxburghii Rroxscaffold_2G00099550 Rroxscaffold_2G00099560 Rroxscaffold_4G00296180 Rroxscaffold_4G00312990 Rroxscaffold_4G00313010 Rroxscaffold_4G00313030 Rroxscaffold_4G00313060
rosa_rugosa Rorug01G0150600.1 Rorug01G0150700.1 Rorug01G0150800.1 Rorug01G0150900.1 Rorug01G0151000.1 Rorug01G0151100.1 Rorug01G0151200.1 Rorug01G0151300.1 Rorug01G0151400.1 Rorug02G0399500 Rorug02G0399600 Rorug02G0399700 Rorug02G0399800 Rorug02G0399800 Rorug02G0399900
rosa_samantha Rh1AG165800 Rh1AG165900 Rh1BG132300 Rh1BG132400 Rh1CG154400 Rh1CG154500 Rh1DG167200 Rh1DG167300 Rh2AG456600 Rh2AG456700 Rh2BG469100 Rh2CG444100 Rh2CG444200 Rh2DG478400 Rh2DG478500
rosa_wichuraiana Rw1G013810 Rw1G013820 Rw1G013860 Rw2G037370 Rw2G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 178
AccB1I GGYRCC 1 cut(s) 178
AccII CGCG 2 cut(s) 522, 555
AciI CCGC 8 cut(s) 53, 547, 704, 707, 721, 733, 785, 857
AclWI GGATC 1 cut(s) 108
AcoI YGGCCR 2 cut(s) 259, 341
AcsI RAATTY 1 cut(s) 335
AfaI GTAC 2 cut(s) 180, 423
AfiI CCNNNNNNNGG 1 cut(s) 350
AflIII ACRYGT 2 cut(s) 77, 119
AgsI TTSAA 4 cut(s) 16, 140, 152, 320
AjiI CACGTC 2 cut(s) 872, 910
AluBI AGCT 3 cut(s) 594, 619, 656
AluI AGCT 3 cut(s) 594, 619, 656
Alw26I GTCTC 2 cut(s) 636, 995
AlwI GGATC 1 cut(s) 108
AoxI GGCC 7 cut(s) 259, 341, 505, 624, 664, 786, 976
ApeKI GCWGC 2 cut(s) 193, 594
ApoI RAATTY 1 cut(s) 335
Asp700I GAANNNNTTC 1 cut(s) 359
Asp718I GGTACC 1 cut(s) 178
AspA2I CCTAGG 1 cut(s) 892
AspLEI GCGC 2 cut(s) 198, 522
AspS9I GGNCC 2 cut(s) 625, 977
AsuHPI GGTGA 4 cut(s) 40, 341, 591, 1061
AvrII CCTAGG 1 cut(s) 892
BalI TGGCCA 2 cut(s) 261, 343
BanI GGYRCC 1 cut(s) 178
BbsI GAAGAC 3 cut(s) 128, 440, 1028
BbvI GCAGC 2 cut(s) 180, 581
BccI CCATC 5 cut(s) 474, 859, 921, 1012, 1069
BcoDI GTCTC 2 cut(s) 636, 995
BfaI CTAG 6 cut(s) 428, 591, 653, 657, 893, 1028
BisI GCNGC 7 cut(s) 194, 595, 704, 707, 721, 734, 786
BlnI CCTAGG 1 cut(s) 892
BlsI GCNGC 7 cut(s) 195, 596, 705, 708, 722, 735, 787
BmgBI CACGTC 2 cut(s) 872, 910
BmgT120I GGNCC 2 cut(s) 625, 977
BmiI GGNNCC 2 cut(s) 180, 979
BmsI GCATC 2 cut(s) 238, 751
BpiI GAAGAC 3 cut(s) 128, 440, 1028
BpuEI CTTGAG 4 cut(s) 149, 194, 631, 641
BsaJI CCNNGG 2 cut(s) 508, 892
BsaWI WCCGGW 1 cut(s) 570
Bsc4I CCNNNNNNNGG 1 cut(s) 350
Bse118I RCCGGY 1 cut(s) 796
Bse3DI GCAATG 2 cut(s) 312, 502
BseDI CCNNGG 2 cut(s) 508, 892
BseGI GGATG 5 cut(s) 485, 742, 851, 913, 919
BseLI CCNNNNNNNGG 1 cut(s) 350
BseMI GCAATG 2 cut(s) 312, 502
BseXI GCAGC 2 cut(s) 180, 581
Bsh1236I CGCG 2 cut(s) 522, 555
Bsh1285I CGRYCG 1 cut(s) 571
BshFI GGCC 7 cut(s) 261, 343, 507, 626, 666, 788, 978
BshNI GGYRCC 1 cut(s) 178
BsiEI CGRYCG 1 cut(s) 571
BsiSI CCGG 2 cut(s) 571, 797
BsiWI CGTACG 1 cut(s) 421
BslI CCNNNNNNNGG 1 cut(s) 350
BsmAI GTCTC 2 cut(s) 636, 995
BsnI GGCC 7 cut(s) 261, 343, 507, 626, 666, 788, 978
Bsp143I GATC 1 cut(s) 100
Bsp68I TCGCGA 1 cut(s) 555
BspACI CCGC 8 cut(s) 53, 547, 704, 707, 721, 733, 785, 857
BspANI GGCC 7 cut(s) 261, 343, 507, 626, 666, 788, 978
BspFNI CGCG 2 cut(s) 522, 555
BspLI GGNNCC 2 cut(s) 180, 979
BspPI GGATC 1 cut(s) 108
BspT107I GGYRCC 1 cut(s) 178
BsrDI GCAATG 2 cut(s) 312, 502
BsrFI RCCGGY 1 cut(s) 796
BssAI RCCGGY 1 cut(s) 796
BssECI CCNNGG 2 cut(s) 508, 892
BssMI GATC 1 cut(s) 100
BssT1I CCWWGG 2 cut(s) 508, 892
Bst4CI ACNGT 3 cut(s) 491, 780, 841
Bst6I CTCTTC 1 cut(s) 147
BstC8I GCNNGC 2 cut(s) 701, 952
BstF5I GGATG 5 cut(s) 485, 742, 851, 913, 919
BstFNI CGCG 2 cut(s) 522, 555
BstHHI GCGC 2 cut(s) 198, 522
BstKTI GATC 1 cut(s) 103
BstMAI GTCTC 2 cut(s) 636, 995
BstMBI GATC 1 cut(s) 100
BstMCI CGRYCG 1 cut(s) 571
BstMWI GCNNNNNNNGC 2 cut(s) 594, 739
BstNSI RCATGY 3 cut(s) 81, 95, 123
BstUI CGCG 2 cut(s) 522, 555
BstV1I GCAGC 2 cut(s) 180, 581
BstV2I GAAGAC 3 cut(s) 128, 440, 1028
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
BsuRI GGCC 7 cut(s) 261, 343, 507, 626, 666, 788, 978
BtrI CACGTC 2 cut(s) 872, 910
BtsCI GGATG 5 cut(s) 485, 742, 851, 913, 919
BtsIMutI CAGTG 1 cut(s) 1014
BtuMI TCGCGA 1 cut(s) 555
Cac8I GCNNGC 2 cut(s) 701, 952
CfoI GCGC 2 cut(s) 198, 522
Cfr10I RCCGGY 1 cut(s) 796
Cfr13I GGNCC 2 cut(s) 625, 977
Csp6I GTAC 2 cut(s) 179, 422
CviAII CATG 6 cut(s) 78, 92, 120, 326, 389, 750
CviQI GTAC 2 cut(s) 179, 422
DpnI GATC 1 cut(s) 102
DpnII GATC 1 cut(s) 100
EaeI YGGCCR 2 cut(s) 259, 341
Eam1104I CTCTTC 1 cut(s) 147
EarI CTCTTC 1 cut(s) 147
Eco130I CCWWGG 2 cut(s) 508, 892
Eco147I AGGCCT 1 cut(s) 666
Eco32I GATATC 1 cut(s) 370
EcoRI GAATTC 1 cut(s) 335
EcoRV GATATC 1 cut(s) 370
EcoT14I CCWWGG 2 cut(s) 508, 892
ErhI CCWWGG 2 cut(s) 508, 892
FaeI CATG 6 cut(s) 81, 95, 123, 329, 392, 753
FatI CATG 6 cut(s) 77, 91, 119, 325, 388, 749
FauI CCCGC 3 cut(s) 60, 540, 864
FauNDI CATATG 1 cut(s) 637
Fnu4HI GCNGC 7 cut(s) 194, 595, 704, 707, 721, 734, 786
FokI GGATG 5 cut(s) 492, 729, 838, 900, 906
Fsp4HI GCNGC 7 cut(s) 194, 595, 704, 707, 721, 734, 786
FspBI CTAG 6 cut(s) 428, 591, 653, 657, 893, 1028
GlaI GCGC 2 cut(s) 197, 521
GluI GCNGC 7 cut(s) 194, 595, 704, 707, 721, 734, 786
HaeIII GGCC 7 cut(s) 261, 343, 507, 626, 666, 788, 978
HapII CCGG 2 cut(s) 571, 797
HhaI GCGC 2 cut(s) 198, 522
Hin1II CATG 6 cut(s) 81, 95, 123, 329, 392, 753
Hin6I GCGC 2 cut(s) 196, 520
HinP1I GCGC 2 cut(s) 196, 520
HincII GTYRAC 2 cut(s) 75, 117
HindII GTYRAC 2 cut(s) 75, 117
HindIII AAGCTT 1 cut(s) 617
HinfI GANTC 9 cut(s) 113, 133, 445, 524, 644, 830, 899, 934, 1012
HpaI GTTAAC 1 cut(s) 75
HpaII CCGG 2 cut(s) 571, 797
HphI GGTGA 4 cut(s) 40, 341, 591, 1061
Hpy166II GTNNAC 2 cut(s) 75, 117
Hpy188I TCNGA 1 cut(s) 1017
Hpy188III TCNNGA 1 cut(s) 554
Hpy8I GTNNAC 2 cut(s) 75, 117
Hpy99I CGWCG 2 cut(s) 780, 883
HpyAV CCTTC 5 cut(s) 574, 669, 689, 756, 991
HpyCH4III ACNGT 3 cut(s) 491, 780, 841
HpyCH4IV ACGT 2 cut(s) 871, 909
HpyCH4V TGCA 3 cut(s) 95, 171, 325
HpyF10VI GCNNNNNNNGC 2 cut(s) 594, 739
HpySE526I ACGT 2 cut(s) 871, 909
Hsp92II CATG 6 cut(s) 81, 95, 123, 329, 392, 753
HspAI GCGC 2 cut(s) 196, 520
KpnI GGTACC 1 cut(s) 182
KspAI GTTAAC 1 cut(s) 75
Kzo9I GATC 1 cut(s) 100
LpnPI CCDG 6 cut(s) 325, 336, 456, 584, 730, 810
Lsp1109I GCAGC 2 cut(s) 180, 581
LweI GCATC 2 cut(s) 238, 751
MaeI CTAG 6 cut(s) 428, 591, 653, 657, 893, 1028
MaeII ACGT 2 cut(s) 871, 909
MaeIII GTNAC 4 cut(s) 122, 841, 935, 1009
MalI GATC 1 cut(s) 102
MboI GATC 1 cut(s) 100
MfeI CAATTG 2 cut(s) 255, 315
MflI RGATCY 1 cut(s) 100
MlsI TGGCCA 2 cut(s) 261, 343
MluCI AATT 5 cut(s) 255, 315, 320, 335, 1067
MluNI TGGCCA 2 cut(s) 261, 343
MlyI GAGTC 7 cut(s) 122, 142, 454, 533, 638, 943, 1006
MmeI TCCRAC 1 cut(s) 796
Mox20I TGGCCA 2 cut(s) 261, 343
MroXI GAANNNNTTC 1 cut(s) 359
MscI TGGCCA 2 cut(s) 261, 343
MseI TTAA 1 cut(s) 74
MslI CAYNNNNRTG 1 cut(s) 330
Msp20I TGGCCA 2 cut(s) 261, 343
MspI CCGG 2 cut(s) 571, 797
MunI CAATTG 2 cut(s) 255, 315
MvnI CGCG 2 cut(s) 522, 555
MwoI GCNNNNNNNGC 2 cut(s) 594, 739
NdeI CATATG 1 cut(s) 637
NdeII GATC 1 cut(s) 100
NlaIII CATG 6 cut(s) 81, 95, 123, 329, 392, 753
NlaIV GGNNCC 2 cut(s) 180, 979
NmeAIII GCCGAG 1 cut(s) 669
NmuCI GTSAC 4 cut(s) 122, 841, 935, 1009
NruI TCGCGA 1 cut(s) 555
NspI RCATGY 3 cut(s) 81, 95, 123
PceI AGGCCT 1 cut(s) 666
PciI ACATGT 2 cut(s) 77, 119
PcsI WCGNNNNNNNCGW 2 cut(s) 241, 707
PdmI GAANNNNTTC 1 cut(s) 359
PfeI GAWTC 2 cut(s) 830, 899
Pfl23II CGTACG 1 cut(s) 421
PkrI GCNGC 7 cut(s) 195, 596, 705, 708, 722, 735, 787
PleI GAGTC 7 cut(s) 121, 141, 453, 532, 638, 942, 1006
PpsI GAGTC 7 cut(s) 121, 141, 453, 532, 638, 942, 1006
PscI ACATGT 2 cut(s) 77, 119
PspLI CGTACG 1 cut(s) 421
PspN4I GGNNCC 2 cut(s) 180, 979
PspPI GGNCC 2 cut(s) 625, 977
PsuI RGATCY 1 cut(s) 100
RruI TCGCGA 1 cut(s) 555
RsaI GTAC 2 cut(s) 180, 423
RsaNI GTAC 2 cut(s) 179, 422
RseI CAYNNNNRTG 1 cut(s) 330
SaqAI TTAA 1 cut(s) 74
SatI GCNGC 7 cut(s) 194, 595, 704, 707, 721, 734, 786
Sau3AI GATC 1 cut(s) 100
Sau96I GGNCC 2 cut(s) 625, 977
SchI GAGTC 7 cut(s) 122, 142, 454, 533, 638, 943, 1006
SfaNI GCATC 2 cut(s) 238, 751
SmiMI CAYNNNNRTG 1 cut(s) 330
SmlI CTYRAG 4 cut(s) 128, 209, 620, 646
SmoI CTYRAG 4 cut(s) 128, 209, 620, 646
Sse9I AATT 5 cut(s) 255, 315, 320, 335, 1067
SseBI AGGCCT 1 cut(s) 666
SsiI CCGC 8 cut(s) 53, 547, 704, 707, 721, 733, 785, 857
SspMI CTAG 6 cut(s) 428, 591, 653, 657, 893, 1028
StuI AGGCCT 1 cut(s) 666
StyI CCWWGG 2 cut(s) 508, 892
TaaI ACNGT 3 cut(s) 491, 780, 841
TaiI ACGT 2 cut(s) 874, 912
TaqI TCGA 4 cut(s) 235, 485, 764, 932
TasI AATT 5 cut(s) 255, 315, 320, 335, 1067
TauI GCSGC 5 cut(s) 706, 709, 723, 736, 788
TfiI GAWTC 2 cut(s) 830, 899
Tru1I TTAA 1 cut(s) 74
Tru9I TTAA 1 cut(s) 74
TscAI CASTG 1 cut(s) 1014
TseFI GTSAC 4 cut(s) 122, 841, 935, 1009
TseI GCWGC 2 cut(s) 193, 594
Tsp45I GTSAC 4 cut(s) 122, 841, 935, 1009
TspDTI ATGAA 3 cut(s) 177, 348, 738
TspGWI ACGGA 1 cut(s) 867
TspRI CASTG 1 cut(s) 1014
XapI RAATTY 1 cut(s) 335
XceI RCATGY 3 cut(s) 81, 95, 123
XcmI CCANNNNNNNNNTGG 2 cut(s) 406, 635
XmaJI CCTAGG 1 cut(s) 892
XmnI GAANNNNTTC 1 cut(s) 359
XspI CTAG 6 cut(s) 428, 591, 653, 657, 893, 1028
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.