MD07G1080300.v1.1

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000623
Physical Location & Seq
Forward (+)
7778130 .. 7780250
2121 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1080300.v1.1.491

Sequence Viewer

Length: 441 bp
ATGGGAGACCAACGTCACCAACCCACATCAAATCCCACGATTGAATTGCAAGGGGATGAGTTTTGGCCACTCTCGAAGAAACCCTTTTTTGAAGTCATCATCAGAAAAGCAAATGTCAAGCCCTCTTATCAAATGGTGATCCCGGCCAAATTTCAACCAACACTACCTTCCTGTTCAATTCCTACGGTTCTCACGTTTGGGGGCAAAAACTGGGAGATGACATACACTGGTGGATCCATTCAGAGAAAGTTCGATATTAACTGGAGAGAATTTGTCAACGACAACAATTTGAAGGTTGGAGATGCATGTGTATTTGAACTTCTGGAGTGCAGCAGCACAAAACTGGCATTCAGAGTCCAAATTCTCAGAGGTGACATCCCATCTGAACTTCTAGGCAATCTCAAGGGTGATACTGTAGATGCACCAATTATTATTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

147

Amino Acids

16.46

Weight (kDa)

5.85

Isoelectric Point (pI)

32.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 30 - 118 2.5e-12 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000623)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g35581 FvH4_6g35581 FvH4_6g35582 FvH4_7g07710 FvH4_7g07720
malus_domestica MD02G1233300.v1.1 MD07G1080300.v1.1
prunus_persica Prupe.2G100300_v2.0.a1 Prupe.2G100300_v2.0.a1 Prupe.2G100300_v2.0.a1 Prupe.2G100300_v2.0.a1 Prupe.2G104300_v2.0.a1 Prupe.2G104700_v2.0.a1 Prupe.2G104700_v2.0.a1 Prupe.2G104700_v2.0.a1
pyrus_communis pycom02g20150 pycom07g06390
rosa_chinensis RchiOBHm_Chr1g0342371 RchiOBHm_Chr1g0342381 RchiOBHm_Chr1g0342401 RchiOBHm_Chr1g0342491 RchiOBHm_Chr1g0342511 RchiOBHm_Chr2g0147401 RchiOBHm_Chr2g0147411
rosa_laevigata RLG00000020253 RLG00000020259 RLG00000029157 RLG00000029158
rosa_multiflora Rmu_sc0000979.1_g000010 Rmu_sc0001159.1_g000034 Rmu_sc0001159.1_g000035 Rmu_sc0005388.1_g000001 Rmu_sc0009535.1_g000001 Rmu_sc0009535.1_g000003 Rmu_sc0011976.1_g000004 Rmu_sc0011976.1_g000005 Rmu_sc0027133.1_g000002
rosa_roxburghii Rroxscaffold_2G00099550 Rroxscaffold_2G00099560 Rroxscaffold_4G00296180 Rroxscaffold_4G00312990 Rroxscaffold_4G00313010 Rroxscaffold_4G00313030 Rroxscaffold_4G00313060
rosa_rugosa Rorug01G0150600.1 Rorug01G0150700.1 Rorug01G0150800.1 Rorug01G0150900.1 Rorug01G0151000.1 Rorug01G0151100.1 Rorug01G0151200.1 Rorug01G0151300.1 Rorug01G0151400.1 Rorug02G0399500 Rorug02G0399600 Rorug02G0399700 Rorug02G0399800 Rorug02G0399800 Rorug02G0399900
rosa_samantha Rh1AG165800 Rh1AG165900 Rh1BG132300 Rh1BG132400 Rh1CG154400 Rh1CG154500 Rh1DG167200 Rh1DG167300 Rh2AG456600 Rh2AG456700 Rh2BG469100 Rh2CG444100 Rh2CG444200 Rh2DG478400 Rh2DG478500
rosa_wichuraiana Rw1G013810 Rw1G013820 Rw1G013860 Rw2G037370 Rw2G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 133, 228, 241
AcoI YGGCCR 2 cut(s) 65, 144
AcsI RAATTY 3 cut(s) 149, 269, 360
AgsI TTSAA 6 cut(s) 44, 92, 155, 177, 292, 317
AjuI GAANNNNNNNTTGG 2 cut(s) 151, 183
AlwI GGATC 3 cut(s) 133, 228, 241
AoxI GGCC 2 cut(s) 65, 144
ApeKI GCWGC 2 cut(s) 330, 333
ApoI RAATTY 3 cut(s) 149, 269, 360
AsuC2I CCSGG 1 cut(s) 143
AsuHPI GGTGA 4 cut(s) 8, 148, 383, 419
BalI TGGCCA 1 cut(s) 67
BamHI GGATCC 1 cut(s) 233
BarI GAAGNNNNNNTAC 2 cut(s) 303, 335
BbvI GCAGC 2 cut(s) 342, 345
BccI CCATC 1 cut(s) 388
BcgI CGANNNNNNTGC 2 cut(s) 28, 62
BcnI CCSGG 1 cut(s) 143
BfaI CTAG 1 cut(s) 392
BfmI CTRYAG 1 cut(s) 414
BisI GCNGC 2 cut(s) 331, 334
BlsI GCNGC 2 cut(s) 332, 335
Bme1390I CCNGG 1 cut(s) 143
BmiI GGNNCC 1 cut(s) 235
BmrFI CCNGG 1 cut(s) 143
BmrI ACTGGG 1 cut(s) 220
BmsI GCATC 2 cut(s) 292, 409
BmuI ACTGGG 1 cut(s) 220
BoxI GACNNNNGTC 1 cut(s) 12
BpmI CTGGAG 2 cut(s) 283, 344
BpuEI CTTGAG 1 cut(s) 386
BpuMI CCSGG 1 cut(s) 143
Bse1I ACTGG 4 cut(s) 215, 232, 266, 348
BseGI GGATG 2 cut(s) 61, 375
BseMII CTCAG 1 cut(s) 379
BseNI ACTGG 4 cut(s) 215, 232, 266, 348
BseXI GCAGC 2 cut(s) 342, 345
BsgI GTGCAG 1 cut(s) 349
BshFI GGCC 2 cut(s) 67, 146
BsiSI CCGG 1 cut(s) 143
BsmI GAATGC 1 cut(s) 347
BsnI GGCC 2 cut(s) 67, 146
Bsp143I GATC 2 cut(s) 138, 233
BspANI GGCC 2 cut(s) 67, 146
BspCNI CTCAG 1 cut(s) 378
BspLI GGNNCC 1 cut(s) 235
BspPI GGATC 3 cut(s) 133, 228, 241
BsrI ACTGG 4 cut(s) 215, 232, 266, 348
BssMI GATC 2 cut(s) 138, 233
Bst4CI ACNGT 2 cut(s) 187, 415
BstDEI CTNAG 1 cut(s) 365
BstF5I GGATG 2 cut(s) 61, 375
BstKTI GATC 2 cut(s) 141, 236
BstMBI GATC 2 cut(s) 138, 233
BstNSI RCATGY 1 cut(s) 309
BstPAI GACNNNNGTC 1 cut(s) 12
BstSCI CCNGG 1 cut(s) 141
BstSFI CTRYAG 1 cut(s) 414
BstV1I GCAGC 2 cut(s) 342, 345
BstX2I RGATCY 1 cut(s) 233
BstYI RGATCY 1 cut(s) 233
BsuRI GGCC 2 cut(s) 67, 146
BtsCI GGATG 2 cut(s) 61, 375
BtsIMutI CAGTG 1 cut(s) 225
CviAII CATG 1 cut(s) 306
CviJI RGCY 3 cut(s) 67, 121, 146
CviKI_1 RGCY 3 cut(s) 67, 121, 146
DdeI CTNAG 1 cut(s) 365
DpnI GATC 2 cut(s) 140, 235
DpnII GATC 2 cut(s) 138, 233
EaeI YGGCCR 2 cut(s) 65, 144
EcoT22I ATGCAT 1 cut(s) 307
FaeI CATG 1 cut(s) 309
FaiI YATR 2 cut(s) 223, 307
FalI AAGNNNNNCTT 2 cut(s) 68, 100
FatI CATG 1 cut(s) 305
Fnu4HI GCNGC 2 cut(s) 331, 334
FokI GGATG 2 cut(s) 68, 362
Fsp4HI GCNGC 2 cut(s) 331, 334
FspBI CTAG 1 cut(s) 392
GluI GCNGC 2 cut(s) 331, 334
GsuI CTGGAG 2 cut(s) 283, 344
HaeIII GGCC 2 cut(s) 67, 146
HapII CCGG 1 cut(s) 143
Hin1II CATG 1 cut(s) 309
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 1 cut(s) 354
HpaII CCGG 1 cut(s) 143
HphI GGTGA 4 cut(s) 8, 148, 383, 419
Hpy166II GTNNAC 1 cut(s) 277
Hpy188I TCNGA 5 cut(s) 104, 243, 353, 368, 385
Hpy188III TCNNGA 2 cut(s) 73, 323
Hpy8I GTNNAC 1 cut(s) 277
HpyAV CCTTC 2 cut(s) 177, 286
HpyCH4III ACNGT 2 cut(s) 187, 415
HpyCH4IV ACGT 2 cut(s) 13, 194
HpyCH4V TGCA 4 cut(s) 49, 305, 330, 422
HpyF3I CTNAG 1 cut(s) 365
HpySE526I ACGT 2 cut(s) 13, 194
Hsp92II CATG 1 cut(s) 309
Kzo9I GATC 2 cut(s) 138, 233
LpnPI CCDG 7 cut(s) 156, 184, 196, 213, 247, 308, 329
Lsp1109I GCAGC 2 cut(s) 342, 345
LweI GCATC 2 cut(s) 292, 409
MaeI CTAG 1 cut(s) 392
MaeII ACGT 2 cut(s) 13, 194
MaeIII GTNAC 2 cut(s) 14, 371
MalI GATC 2 cut(s) 140, 235
MboI GATC 2 cut(s) 138, 233
MboII GAAGA 1 cut(s) 88
MflI RGATCY 1 cut(s) 233
MlsI TGGCCA 1 cut(s) 67
MluCI AATT 7 cut(s) 44, 149, 177, 269, 286, 360, 426
MluNI TGGCCA 1 cut(s) 67
MlyI GAGTC 1 cut(s) 363
MmeI TCCRAC 1 cut(s) 277
MnlI CCTC 2 cut(s) 133, 362
Mox20I TGGCCA 1 cut(s) 67
Mph1103I ATGCAT 1 cut(s) 307
MscI TGGCCA 1 cut(s) 67
MseI TTAA 1 cut(s) 258
Msp20I TGGCCA 1 cut(s) 67
MspI CCGG 1 cut(s) 143
MspR9I CCNGG 1 cut(s) 143
Mva1269I GAATGC 1 cut(s) 347
NciI CCSGG 1 cut(s) 143
NdeII GATC 2 cut(s) 138, 233
NlaIII CATG 1 cut(s) 309
NlaIV GGNNCC 1 cut(s) 235
NmuCI GTSAC 2 cut(s) 14, 371
NsiI ATGCAT 1 cut(s) 307
NspI RCATGY 1 cut(s) 309
PcsI WCGNNNNNNNCGW 1 cut(s) 191
PctI GAATGC 1 cut(s) 347
PkrI GCNGC 2 cut(s) 332, 335
PleI GAGTC 1 cut(s) 362
PpsI GAGTC 1 cut(s) 362
PshAI GACNNNNGTC 1 cut(s) 12
PspN4I GGNNCC 1 cut(s) 235
PsuI RGATCY 1 cut(s) 233
SaqAI TTAA 1 cut(s) 258
SatI GCNGC 2 cut(s) 331, 334
Sau3AI GATC 2 cut(s) 138, 233
SchI GAGTC 1 cut(s) 363
ScrFI CCNGG 1 cut(s) 143
SetI ASST 5 cut(s) 16, 169, 197, 297, 373
SfaNI GCATC 2 cut(s) 292, 409
SfcI CTRYAG 1 cut(s) 414
SmlI CTYRAG 1 cut(s) 401
SmoI CTYRAG 1 cut(s) 401
Sse9I AATT 7 cut(s) 44, 149, 177, 269, 286, 360, 426
SspMI CTAG 1 cut(s) 392
StyD4I CCNGG 1 cut(s) 141
TaaI ACNGT 2 cut(s) 187, 415
TaiI ACGT 2 cut(s) 16, 197
TaqI TCGA 2 cut(s) 74, 252
TasI AATT 7 cut(s) 44, 149, 177, 269, 286, 360, 426
Tru1I TTAA 1 cut(s) 258
Tru9I TTAA 1 cut(s) 258
TscAI CASTG 1 cut(s) 232
TseFI GTSAC 2 cut(s) 14, 371
TseI GCWGC 2 cut(s) 330, 333
Tsp45I GTSAC 2 cut(s) 14, 371
TspRI CASTG 1 cut(s) 232
XapI RAATTY 3 cut(s) 149, 269, 360
XceI RCATGY 1 cut(s) 309
XspI CTAG 1 cut(s) 392
Zsp2I ATGCAT 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.