FvH4_6g43330

Protein of unknown function (DUF2781)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001495
Physical Location & Seq
Forward (+)
33555364 .. 33557212
1849 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g43330.t1

Sequence Viewer

Length: 513 bp
ATGGGCGCTCTTGTCAAGCTCATGGATGCTTTGCTCTTCATCTTGCTCCTTGCGATCGCTTTCAAAATCCCATTGCTTAAAGGGCAAGCATGCCTCCCAATCACCCTCTACCCCGATTGTCTCGTTGAACTCCGGAGCTGGTATGCCGGAGAGGTTGGCGACTACCTCATGACGGAGACGCCGGATTTCTACATAGGGATCATATGGGTTGAGCTTCTCTTTCAATGGCCTCTGGTGCTTGTGAATCTTTATGGCATTTTGGCAGGGAAGCATTGGTTCAACACCACCTGCTTGAGTTATGGCATTTCTCTATTCACTGGGATGGTTCCAATATTTGCAGAACTCACAAGATCAGGAAGGGCACCGGAAAAGTTGTTGACGGTGTACTATACGTTCTTGAGTTTGGGTGTTCTTGCCATACTTCGTGGATTGCTTCCCAACTCATCAACAACCAAGTTCTCTTCTGCAACCTTTGGCAAGAGACCTGCCTCGGGTAGGAAGAAGAGAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2781

Protein Analysis

171

Amino Acids

18.95

Weight (kDa)

9.32

Isoelectric Point (pI)

34.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EBP PF05241 41 - 118 1.7e-10 EXPERA (EXPanded EBP superfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 296
Acc36I ACCTGC 2 cut(s) 296, 493
AccB1I GGYRCC 1 cut(s) 361
AccIII TCCGGA 1 cut(s) 132
AclWI GGATC 1 cut(s) 206
AcyI GRCGYC 1 cut(s) 179
AfaI GTAC 1 cut(s) 386
AfiI CCNNNNNNNGG 3 cut(s) 172, 491, 495
AgsI TTSAA 4 cut(s) 64, 128, 224, 280
AluBI AGCT 3 cut(s) 19, 138, 214
AluI AGCT 3 cut(s) 19, 138, 214
Alw26I GTCTC 3 cut(s) 125, 170, 475
AlwI GGATC 1 cut(s) 206
Ama87I CYCGRG 1 cut(s) 490
Aor13HI TCCGGA 1 cut(s) 132
AoxI GGCC 1 cut(s) 227
AsiSI GCGATCGC 1 cut(s) 57
AspLEI GCGC 1 cut(s) 8
AsuHPI GGTGA 1 cut(s) 94
AvaI CYCGRG 1 cut(s) 490
BaeGI GKGCMC 1 cut(s) 364
BanI GGYRCC 1 cut(s) 361
BccI CCATC 1 cut(s) 316
BcoDI GTCTC 3 cut(s) 125, 170, 475
BfoI RGCGCY 1 cut(s) 9
BfuAI ACCTGC 2 cut(s) 296, 493
BmeT110I CYCGRG 1 cut(s) 490
BmiI GGNNCC 2 cut(s) 327, 363
BmrI ACTGGG 1 cut(s) 327
BmsI GCATC 1 cut(s) 16
BmuI ACTGGG 1 cut(s) 327
BpuEI CTTGAG 2 cut(s) 313, 418
BsaHI GRCGYC 1 cut(s) 179
BsaI GGTCTC 1 cut(s) 475
BsaJI CCNNGG 1 cut(s) 489
BsaWI WCCGGW 2 cut(s) 132, 364
Bsc4I CCNNNNNNNGG 3 cut(s) 172, 491, 495
Bse1I ACTGG 1 cut(s) 322
Bse3DI GCAATG 1 cut(s) 71
BseAI TCCGGA 1 cut(s) 132
BseDI CCNNGG 1 cut(s) 489
BseGI GGATG 2 cut(s) 31, 327
BseLI CCNNNNNNNGG 3 cut(s) 172, 491, 495
BseMI GCAATG 1 cut(s) 71
BseNI ACTGG 1 cut(s) 322
BseSI GKGCMC 1 cut(s) 364
Bsh1285I CGRYCG 1 cut(s) 57
BshFI GGCC 1 cut(s) 229
BshNI GGYRCC 1 cut(s) 361
BsiEI CGRYCG 1 cut(s) 57
BsiHKCI CYCGRG 1 cut(s) 490
BsiSI CCGG 4 cut(s) 133, 147, 182, 365
BslI CCNNNNNNNGG 3 cut(s) 172, 491, 495
BsmAI GTCTC 3 cut(s) 125, 170, 475
BsmBI CGTCTC 1 cut(s) 170
BsnI GGCC 1 cut(s) 229
Bso31I GGTCTC 1 cut(s) 475
BsoBI CYCGRG 1 cut(s) 490
Bsp1286I GDGCHC 1 cut(s) 364
Bsp13I TCCGGA 1 cut(s) 132
Bsp143I GATC 3 cut(s) 54, 198, 350
BspANI GGCC 1 cut(s) 229
BspEI TCCGGA 1 cut(s) 132
BspHI TCATGA 1 cut(s) 168
BspLI GGNNCC 2 cut(s) 327, 363
BspMI ACCTGC 2 cut(s) 296, 493
BspPI GGATC 1 cut(s) 206
BspQI GCTCTTC 1 cut(s) 41
BspT107I GGYRCC 1 cut(s) 361
BspTNI GGTCTC 1 cut(s) 475
BsrDI GCAATG 1 cut(s) 71
BsrI ACTGG 1 cut(s) 322
BssECI CCNNGG 1 cut(s) 489
BssMI GATC 3 cut(s) 54, 198, 350
BssNI GRCGYC 1 cut(s) 179
Bst4CI ACNGT 1 cut(s) 382
Bst6I CTCTTC 3 cut(s) 41, 466, 497
BstACI GRCGYC 1 cut(s) 179
BstC8I GCNNGC 2 cut(s) 87, 91
BstENI CCTNNNNNAGG 1 cut(s) 493
BstF5I GGATG 2 cut(s) 31, 327
BstH2I RGCGCY 1 cut(s) 9
BstHHI GCGC 1 cut(s) 8
BstKTI GATC 3 cut(s) 57, 201, 353
BstMAI GTCTC 3 cut(s) 125, 170, 475
BstMBI GATC 3 cut(s) 54, 198, 350
BstMCI CGRYCG 1 cut(s) 57
BstMWI GCNNNNNNNGC 2 cut(s) 82, 235
BstNSI RCATGY 1 cut(s) 93
BstSLI GKGCMC 1 cut(s) 364
BsuRI GGCC 1 cut(s) 229
BtsCI GGATG 2 cut(s) 31, 327
BtsIMutI CAGTG 1 cut(s) 315
BveI ACCTGC 2 cut(s) 296, 493
Cac8I GCNNGC 2 cut(s) 87, 91
CciI TCATGA 1 cut(s) 168
CfoI GCGC 1 cut(s) 8
CseI GACGC 1 cut(s) 187
Csp6I GTAC 1 cut(s) 385
CviAII CATG 3 cut(s) 22, 90, 169
CviJI RGCY 4 cut(s) 19, 138, 214, 229
CviKI_1 RGCY 4 cut(s) 19, 138, 214, 229
CviQI GTAC 1 cut(s) 385
DpnI GATC 3 cut(s) 56, 200, 352
DpnII GATC 3 cut(s) 54, 198, 350
Eam1104I CTCTTC 3 cut(s) 41, 466, 497
EarI CTCTTC 3 cut(s) 41, 466, 497
Eco31I GGTCTC 1 cut(s) 475
Eco88I CYCGRG 1 cut(s) 490
EcoNI CCTNNNNNAGG 1 cut(s) 493
Esp3I CGTCTC 1 cut(s) 170
FaeI CATG 3 cut(s) 25, 93, 172
FatI CATG 3 cut(s) 21, 89, 168
FauNDI CATATG 1 cut(s) 203
FokI GGATG 2 cut(s) 38, 334
GlaI GCGC 1 cut(s) 7
HaeII RGCGCY 1 cut(s) 9
HaeIII GGCC 1 cut(s) 229
HapII CCGG 4 cut(s) 133, 147, 182, 365
HgaI GACGC 1 cut(s) 187
HhaI GCGC 1 cut(s) 8
Hin1I GRCGYC 1 cut(s) 179
Hin1II CATG 3 cut(s) 25, 93, 172
Hin6I GCGC 1 cut(s) 6
HinP1I GCGC 1 cut(s) 6
HincII GTYRAC 1 cut(s) 378
HindII GTYRAC 1 cut(s) 378
HinfI GANTC 1 cut(s) 244
HpaII CCGG 4 cut(s) 133, 147, 182, 365
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 2 cut(s) 378, 385
Hpy188III TCNNGA 4 cut(s) 133, 169, 354, 397
Hpy8I GTNNAC 2 cut(s) 378, 385
HpyAV CCTTC 1 cut(s) 351
HpyCH4III ACNGT 1 cut(s) 382
HpyCH4IV ACGT 1 cut(s) 392
HpyCH4V TGCA 2 cut(s) 338, 467
HpyF10VI GCNNNNNNNGC 2 cut(s) 82, 235
HpySE526I ACGT 1 cut(s) 392
Hsp92I GRCGYC 1 cut(s) 179
Hsp92II CATG 3 cut(s) 25, 93, 172
HspAI GCGC 1 cut(s) 6
Kpn2I TCCGGA 1 cut(s) 132
Kzo9I GATC 3 cut(s) 54, 198, 350
LguI GCTCTTC 1 cut(s) 41
LmnI GCTCC 2 cut(s) 51, 135
LweI GCATC 1 cut(s) 16
MaeII ACGT 1 cut(s) 392
MalI GATC 3 cut(s) 56, 200, 352
MboI GATC 3 cut(s) 54, 198, 350
MboII GAAGA 3 cut(s) 28, 453, 511
MhlI GDGCHC 1 cut(s) 364
MnlI CCTC 6 cut(s) 104, 116, 145, 176, 240, 499
MroI TCCGGA 1 cut(s) 132
MseI TTAA 1 cut(s) 78
MslI CAYNNNNRTG 1 cut(s) 320
MspI CCGG 4 cut(s) 133, 147, 182, 365
MwoI GCNNNNNNNGC 2 cut(s) 82, 235
NdeI CATATG 1 cut(s) 203
NdeII GATC 3 cut(s) 54, 198, 350
NlaIII CATG 3 cut(s) 25, 93, 172
NlaIV GGNNCC 2 cut(s) 327, 363
NspI RCATGY 1 cut(s) 93
PaeI GCATGC 1 cut(s) 93
PagI TCATGA 1 cut(s) 168
PaqCI CACCTGC 1 cut(s) 296
PciSI GCTCTTC 1 cut(s) 41
PfeI GAWTC 1 cut(s) 244
Ple19I CGATCG 1 cut(s) 57
PspN4I GGNNCC 2 cut(s) 327, 363
PsrI GAACNNNNNNTAC 2 cut(s) 377, 409
PvuI CGATCG 1 cut(s) 57
RgaI GCGATCGC 1 cut(s) 57
RsaI GTAC 1 cut(s) 386
RsaNI GTAC 1 cut(s) 385
RseI CAYNNNNRTG 1 cut(s) 320
SapI GCTCTTC 1 cut(s) 41
SaqAI TTAA 1 cut(s) 78
Sau3AI GATC 3 cut(s) 54, 198, 350
SduI GDGCHC 1 cut(s) 364
SetI ASST 9 cut(s) 21, 140, 156, 168, 216, 290, 395, 473, 487
SfaAI GCGATCGC 1 cut(s) 57
SfaNI GCATC 1 cut(s) 16
SgfI GCGATCGC 1 cut(s) 57
SmiMI CAYNNNNRTG 1 cut(s) 320
SmlI CTYRAG 2 cut(s) 292, 397
SmoI CTYRAG 2 cut(s) 292, 397
SphI GCATGC 1 cut(s) 93
SspI AATATT 1 cut(s) 333
TaaI ACNGT 1 cut(s) 382
TaiI ACGT 1 cut(s) 395
TatI WGTACW 1 cut(s) 384
TfiI GAWTC 1 cut(s) 244
Tru1I TTAA 1 cut(s) 78
Tru9I TTAA 1 cut(s) 78
TscAI CASTG 1 cut(s) 322
TspDTI ATGAA 1 cut(s) 28
TspGWI ACGGA 1 cut(s) 188
TspRI CASTG 1 cut(s) 322
XagI CCTNNNNNAGG 1 cut(s) 493
XceI RCATGY 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.