Rh2AG544800

Transmembrane protein 97-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0001495
Physical Location & Seq
Forward (+)
77316182 .. 77318922
2741 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG544800.1

Sequence Viewer

Length: 384 bp
ATGGGTGCTCTGTTAAAGCTTGTGGACGCCGTCCTCTTCGTCTTCTTCGTCGTAATCGCGGTGGCGGCGCCGTTAATCGACGCGCAGACGTGTCTGCCTGAGAGCCTCTTCCCAAGCGTCTTAGTTGAGCACAAGAACTGGTACGCGCGTGAGTACGGCGACTATCTCATGACCCAGAAGCCTCATTTCATCGTTGGGATCGTCTGGCTTGAGCTGCTGTTTCAGTGGCCTCTTGCTCTTGCCAACCTGTACGGAATTTTGGCCTCCAAGTCTTGGTTTAGCACCACCTGCTTGATCTATGGCGTCTCTGTTTTCACCTCCATGGTAAAGTTTCCAACTTTCTTTTTTTACTTTTACTTGTATCCAATTCCTTGTTTTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2781

Protein Analysis

127

Amino Acids

14.65

Weight (kDa)

6.02

Isoelectric Point (pI)

26.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EBP PF05241 43 - 118 1.5e-10 EXPERA (EXPanded EBP superfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 296
Acc36I ACCTGC 1 cut(s) 296
AccB1I GGYRCC 1 cut(s) 67
AccB7I CCANNNNNTGG 1 cut(s) 273
AccII CGCG 4 cut(s) 59, 83, 146, 148
AciI CCGC 2 cut(s) 59, 65
AclWI GGATC 1 cut(s) 206
AcsI RAATTY 1 cut(s) 255
AcyI GRCGYC 3 cut(s) 27, 68, 303
AfaI GTAC 3 cut(s) 143, 155, 251
AfiI CCNNNNNNNGG 1 cut(s) 273
AflIII ACRYGT 1 cut(s) 89
AjiI CACGTC 1 cut(s) 90
AluBI AGCT 2 cut(s) 19, 214
AluI AGCT 2 cut(s) 19, 214
Alw21I GWGCWC 2 cut(s) 10, 132
Alw26I GTCTC 1 cut(s) 310
AlwI GGATC 1 cut(s) 206
AoxI GGCC 2 cut(s) 227, 261
ApeKI GCWGC 1 cut(s) 214
ApoI RAATTY 1 cut(s) 255
AspLEI GCGC 3 cut(s) 70, 85, 148
AsuHPI GGTGA 1 cut(s) 307
BaeI ACNNNNGTAYC 2 cut(s) 133, 166
BanI GGYRCC 1 cut(s) 67
BbsI GAAGAC 1 cut(s) 34
Bbv12I GWGCWC 2 cut(s) 10, 132
BbvI GCAGC 1 cut(s) 201
BceAI ACGGC 3 cut(s) 14, 55, 172
BciVI GTATCC 1 cut(s) 372
BcoDI GTCTC 1 cut(s) 310
BfoI RGCGCY 1 cut(s) 71
BfuAI ACCTGC 1 cut(s) 296
BfuI GTATCC 1 cut(s) 372
BisI GCNGC 2 cut(s) 66, 215
BlsI GCNGC 2 cut(s) 67, 216
BmgBI CACGTC 1 cut(s) 90
BmiI GGNNCC 1 cut(s) 69
BpiI GAAGAC 1 cut(s) 34
BpuEI CTTGAG 1 cut(s) 230
BsaHI GRCGYC 3 cut(s) 27, 68, 303
BsaJI CCNNGG 1 cut(s) 321
Bsc4I CCNNNNNNNGG 1 cut(s) 273
Bse1I ACTGG 1 cut(s) 143
BseDI CCNNGG 1 cut(s) 321
BseLI CCNNNNNNNGG 1 cut(s) 273
BseMII CTCAG 1 cut(s) 90
BseNI ACTGG 1 cut(s) 143
BseXI GCAGC 1 cut(s) 201
Bsh1236I CGCG 4 cut(s) 59, 83, 146, 148
BshFI GGCC 2 cut(s) 229, 263
BshNI GGYRCC 1 cut(s) 67
BsiHKAI GWGCWC 2 cut(s) 10, 132
BslI CCNNNNNNNGG 1 cut(s) 273
BsmAI GTCTC 1 cut(s) 310
BsmBI CGTCTC 1 cut(s) 310
BsnI GGCC 2 cut(s) 229, 263
Bsp1286I GDGCHC 2 cut(s) 10, 132
Bsp143I GATC 2 cut(s) 198, 294
Bsp19I CCATGG 1 cut(s) 321
BspACI CCGC 2 cut(s) 59, 65
BspANI GGCC 2 cut(s) 229, 263
BspCNI CTCAG 1 cut(s) 91
BspFNI CGCG 4 cut(s) 59, 83, 146, 148
BspHI TCATGA 1 cut(s) 168
BspLI GGNNCC 1 cut(s) 69
BspMI ACCTGC 1 cut(s) 296
BspPI GGATC 1 cut(s) 206
BspT107I GGYRCC 1 cut(s) 67
BsrI ACTGG 1 cut(s) 143
BssECI CCNNGG 1 cut(s) 321
BssMI GATC 2 cut(s) 198, 294
BssNI GRCGYC 3 cut(s) 27, 68, 303
BssT1I CCWWGG 1 cut(s) 321
Bst6I CTCTTC 2 cut(s) 41, 113
BstACI GRCGYC 3 cut(s) 27, 68, 303
BstAPI GCANNNNNTGC 1 cut(s) 288
BstDEI CTNAG 2 cut(s) 99, 121
BstDSI CCRYGG 1 cut(s) 321
BstFNI CGCG 4 cut(s) 59, 83, 146, 148
BstH2I RGCGCY 1 cut(s) 71
BstHHI GCGC 3 cut(s) 70, 85, 148
BstKTI GATC 2 cut(s) 201, 297
BstMAI GTCTC 1 cut(s) 310
BstMBI GATC 2 cut(s) 198, 294
BstMWI GCNNNNNNNGC 3 cut(s) 65, 214, 288
BstUI CGCG 4 cut(s) 59, 83, 146, 148
BstV1I GCAGC 1 cut(s) 201
BstV2I GAAGAC 1 cut(s) 34
BsuI GTATCC 1 cut(s) 372
BsuRI GGCC 2 cut(s) 229, 263
BtgI CCRYGG 1 cut(s) 321
BtrI CACGTC 1 cut(s) 90
BtsIMutI CAGTG 1 cut(s) 230
BveI ACCTGC 1 cut(s) 296
CciI TCATGA 1 cut(s) 168
CfoI GCGC 3 cut(s) 70, 85, 148
CseI GACGC 4 cut(s) 35, 89, 106, 292
Csp6I GTAC 3 cut(s) 142, 154, 250
CviAII CATG 2 cut(s) 169, 322
CviJI RGCY 7 cut(s) 19, 105, 181, 208, 214, 229, 263
CviKI_1 RGCY 7 cut(s) 19, 105, 181, 208, 214, 229, 263
CviQI GTAC 3 cut(s) 142, 154, 250
DdeI CTNAG 2 cut(s) 99, 121
DinI GGCGCC 1 cut(s) 69
DpnI GATC 2 cut(s) 200, 296
DpnII GATC 2 cut(s) 198, 294
Eam1104I CTCTTC 2 cut(s) 41, 113
EarI CTCTTC 2 cut(s) 41, 113
Eco130I CCWWGG 1 cut(s) 321
EcoT14I CCWWGG 1 cut(s) 321
EgeI GGCGCC 1 cut(s) 69
EheI GGCGCC 1 cut(s) 69
ErhI CCWWGG 1 cut(s) 321
Esp3I CGTCTC 1 cut(s) 310
FaeI CATG 2 cut(s) 172, 325
FaiI YATR 3 cut(s) 170, 300, 323
FatI CATG 2 cut(s) 168, 321
Fnu4HI GCNGC 2 cut(s) 66, 215
Fsp4HI GCNGC 2 cut(s) 66, 215
GlaI GCGC 3 cut(s) 69, 84, 147
GluI GCNGC 2 cut(s) 66, 215
HaeII RGCGCY 1 cut(s) 71
HaeIII GGCC 2 cut(s) 229, 263
HgaI GACGC 4 cut(s) 35, 89, 106, 292
HhaI GCGC 3 cut(s) 70, 85, 148
Hin1I GRCGYC 3 cut(s) 27, 68, 303
Hin1II CATG 2 cut(s) 172, 325
Hin6I GCGC 3 cut(s) 68, 83, 146
HinP1I GCGC 3 cut(s) 68, 83, 146
HindIII AAGCTT 1 cut(s) 17
HphI GGTGA 1 cut(s) 307
Hpy166II GTNNAC 1 cut(s) 25
Hpy188III TCNNGA 1 cut(s) 169
Hpy8I GTNNAC 1 cut(s) 25
Hpy99I CGWCG 2 cut(s) 53, 83
HpyCH4IV ACGT 1 cut(s) 89
HpyF10VI GCNNNNNNNGC 3 cut(s) 65, 214, 288
HpyF3I CTNAG 2 cut(s) 99, 121
HpySE526I ACGT 1 cut(s) 89
Hsp92I GRCGYC 3 cut(s) 27, 68, 303
Hsp92II CATG 2 cut(s) 172, 325
HspAI GCGC 3 cut(s) 68, 83, 146
KasI GGCGCC 1 cut(s) 67
Kzo9I GATC 2 cut(s) 198, 294
LpnPI CCDG 6 cut(s) 111, 124, 188, 190, 260, 301
Lsp1109I GCAGC 1 cut(s) 201
MaeII ACGT 1 cut(s) 89
MalI GATC 2 cut(s) 200, 296
MboI GATC 2 cut(s) 198, 294
MboII GAAGA 4 cut(s) 28, 34, 37, 100
MhlI GDGCHC 2 cut(s) 10, 132
MluCI AATT 2 cut(s) 255, 366
Mly113I GGCGCC 1 cut(s) 68
MmeI TCCRAC 1 cut(s) 359
MnlI CCTC 6 cut(s) 44, 116, 192, 240, 274, 328
MseI TTAA 2 cut(s) 14, 74
MslI CAYNNNNRTG 1 cut(s) 320
MvnI CGCG 4 cut(s) 59, 83, 146, 148
MwoI GCNNNNNNNGC 3 cut(s) 65, 214, 288
NarI GGCGCC 1 cut(s) 68
NcoI CCATGG 1 cut(s) 321
NdeII GATC 2 cut(s) 198, 294
NlaIII CATG 2 cut(s) 172, 325
NlaIV GGNNCC 1 cut(s) 69
PagI TCATGA 1 cut(s) 168
PaqCI CACCTGC 1 cut(s) 296
PcsI WCGNNNNNNNCGW 2 cut(s) 45, 198
PflFI GACNNNGTC 1 cut(s) 29
PflMI CCANNNNNTGG 1 cut(s) 273
PkrI GCNGC 2 cut(s) 67, 216
PluTI GGCGCC 1 cut(s) 71
PspN4I GGNNCC 1 cut(s) 69
PsyI GACNNNGTC 1 cut(s) 29
RsaI GTAC 3 cut(s) 143, 155, 251
RsaNI GTAC 3 cut(s) 142, 154, 250
RseI CAYNNNNRTG 1 cut(s) 320
SaqAI TTAA 2 cut(s) 14, 74
SatI GCNGC 2 cut(s) 66, 215
Sau3AI GATC 2 cut(s) 198, 294
SduI GDGCHC 2 cut(s) 10, 132
SetI ASST 6 cut(s) 21, 92, 216, 249, 290, 320
SfoI GGCGCC 1 cut(s) 69
SmiMI CAYNNNNRTG 1 cut(s) 320
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
Sse9I AATT 2 cut(s) 255, 366
SsiI CCGC 2 cut(s) 59, 65
SspDI GGCGCC 1 cut(s) 67
StyI CCWWGG 1 cut(s) 321
TaiI ACGT 1 cut(s) 92
TaqI TCGA 1 cut(s) 78
TasI AATT 2 cut(s) 255, 366
TauI GCSGC 1 cut(s) 68
Tru1I TTAA 2 cut(s) 14, 74
Tru9I TTAA 2 cut(s) 14, 74
TscAI CASTG 1 cut(s) 230
TseI GCWGC 1 cut(s) 214
TspDTI ATGAA 1 cut(s) 178
TspGWI ACGGA 1 cut(s) 267
TspRI CASTG 1 cut(s) 230
Tth111I GACNNNGTC 1 cut(s) 29
Van91I CCANNNNNTGG 1 cut(s) 273
XapI RAATTY 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.