RchiOBHm_Chr2g0159721

Transmembrane protein 97-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001495
Physical Location & Seq
Forward (+)
75743287 .. 75744822
1536 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52828

Sequence Viewer

Length: 660 bp
ATGAAAAGCATCAAGCCAAACGATAGTCGTCGAAATTGCGAAACTCGACTTCCAAGACGCATATCATCTCCTTCCTCCCGCACACAGTTTCACAGAACCCCCCAAGAGAAACACAGACGCCCATACAAAAAGCGCAATTACCTTCATACCCTCCAAATGGGTGCTCTGTTAAAGCTTGTGGACGCCGTCCTCTTCGTCTTCTTCGTCGTAATCGCGGTGGCGGCGCCGTTAATCGACGCGCAGACGTGTCTGCCTGAGAGCCTCTTCCCGAGCGTCTTAGTTGAGCTCAAGAACTGGTACGCGCGTGAGTACGGCGACTATCTCATGACCCAGAAGCCTCATTTCATCGTTGGGATCGTCTGGCTTGAGCTGCTGTTTCAGTGGCCTCTTGCTCTTGCCAACCTGTACGGAATTTTGGCCTCCAAGTCTTGGTTTAGCACCACCTGCTTGATCTATGGCGTCTCTGTTTTCACCTCCATGGTGACTATATTATCAGAACTGATTCAGTCTGGCAAGGCTTCTGATAAGCTGTTGTTTATGTACTTCCCTTTCCTGGGGTTGGGTGTTTTGGCCATACTGCGTGGGCTGCTGACACCTTCCAGGACGAGTAGTTCAATGATCGGCAAGAGACCTATGTTGGGCAGGAAAAAGAAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2781

Protein Analysis

219

Amino Acids

24.96

Weight (kDa)

10.19

Isoelectric Point (pI)

44.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EBP PF05241 93 - 192 1.5e-14 EXPERA (EXPanded EBP superfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 452
Acc36I ACCTGC 1 cut(s) 452
AccB1I GGYRCC 1 cut(s) 223
AccB7I CCANNNNNTGG 1 cut(s) 429
AccII CGCG 4 cut(s) 215, 239, 302, 304
AciI CCGC 3 cut(s) 79, 215, 221
AclWI GGATC 1 cut(s) 362
AcoI YGGCCR 1 cut(s) 570
AcsI RAATTY 1 cut(s) 411
AcyI GRCGYC 4 cut(s) 118, 183, 224, 459
AfaI GTAC 4 cut(s) 299, 311, 407, 542
AfiI CCNNNNNNNGG 6 cut(s) 157, 429, 553, 554, 559, 638
AflIII ACRYGT 1 cut(s) 245
AgsI TTSAA 1 cut(s) 615
AjiI CACGTC 1 cut(s) 246
AjnI CCWGG 2 cut(s) 552, 599
AloI GAACNNNNNNTCC 2 cut(s) 595, 627
AluBI AGCT 4 cut(s) 175, 286, 370, 529
AluI AGCT 4 cut(s) 175, 286, 370, 529
Alw21I GWGCWC 2 cut(s) 166, 288
Alw26I GTCTC 2 cut(s) 466, 622
AlwI GGATC 1 cut(s) 362
Ama87I CYCGRG 1 cut(s) 268
AoxI GGCC 3 cut(s) 383, 417, 570
ApeKI GCWGC 2 cut(s) 370, 586
ApoI RAATTY 1 cut(s) 411
Asp700I GAANNNNTTC 1 cut(s) 501
AspLEI GCGC 4 cut(s) 135, 226, 241, 304
AsuHPI GGTGA 2 cut(s) 463, 493
AvaI CYCGRG 1 cut(s) 268
BaeI ACNNNNGTAYC 2 cut(s) 289, 322
BalI TGGCCA 1 cut(s) 572
BanI GGYRCC 1 cut(s) 223
BanII GRGCYC 1 cut(s) 288
BbsI GAAGAC 1 cut(s) 190
Bbv12I GWGCWC 2 cut(s) 166, 288
BbvI GCAGC 2 cut(s) 357, 573
BceAI ACGGC 3 cut(s) 170, 211, 328
BciT130I CCWGG 2 cut(s) 554, 601
BcoDI GTCTC 2 cut(s) 466, 622
BfoI RGCGCY 1 cut(s) 227
BfuAI ACCTGC 1 cut(s) 452
BisI GCNGC 3 cut(s) 222, 371, 587
BlsI GCNGC 3 cut(s) 223, 372, 588
Bme1390I CCNGG 2 cut(s) 554, 601
BmeT110I CYCGRG 1 cut(s) 268
BmgBI CACGTC 1 cut(s) 246
BmiI GGNNCC 1 cut(s) 225
BmrFI CCNGG 2 cut(s) 554, 601
BmsI GCATC 1 cut(s) 18
BpiI GAAGAC 1 cut(s) 190
BpuEI CTTGAG 2 cut(s) 272, 386
BsaHI GRCGYC 4 cut(s) 118, 183, 224, 459
BsaI GGTCTC 1 cut(s) 622
BsaJI CCNNGG 2 cut(s) 477, 553
Bsc4I CCNNNNNNNGG 6 cut(s) 157, 429, 553, 554, 559, 638
Bse1I ACTGG 1 cut(s) 299
BseBI CCWGG 2 cut(s) 554, 601
BseDI CCNNGG 2 cut(s) 477, 553
BseLI CCNNNNNNNGG 6 cut(s) 157, 429, 553, 554, 559, 638
BseMII CTCAG 1 cut(s) 246
BseNI ACTGG 1 cut(s) 299
BseXI GCAGC 2 cut(s) 357, 573
Bsh1236I CGCG 4 cut(s) 215, 239, 302, 304
BshFI GGCC 3 cut(s) 385, 419, 572
BshNI GGYRCC 1 cut(s) 223
BsiHKAI GWGCWC 2 cut(s) 166, 288
BsiHKCI CYCGRG 1 cut(s) 268
BslI CCNNNNNNNGG 6 cut(s) 157, 429, 553, 554, 559, 638
BsmAI GTCTC 2 cut(s) 466, 622
BsmBI CGTCTC 1 cut(s) 466
BsnI GGCC 3 cut(s) 385, 419, 572
Bso31I GGTCTC 1 cut(s) 622
BsoBI CYCGRG 1 cut(s) 268
Bsp1286I GDGCHC 2 cut(s) 166, 288
Bsp143I GATC 3 cut(s) 354, 450, 618
Bsp19I CCATGG 1 cut(s) 477
BspACI CCGC 3 cut(s) 79, 215, 221
BspANI GGCC 3 cut(s) 385, 419, 572
BspCNI CTCAG 1 cut(s) 247
BspFNI CGCG 4 cut(s) 215, 239, 302, 304
BspHI TCATGA 1 cut(s) 324
BspLI GGNNCC 1 cut(s) 225
BspMI ACCTGC 1 cut(s) 452
BspPI GGATC 1 cut(s) 362
BspT107I GGYRCC 1 cut(s) 223
BspTNI GGTCTC 1 cut(s) 622
BsrI ACTGG 1 cut(s) 299
BssECI CCNNGG 2 cut(s) 477, 553
BssMI GATC 3 cut(s) 354, 450, 618
BssNI GRCGYC 4 cut(s) 118, 183, 224, 459
BssT1I CCWWGG 1 cut(s) 477
Bst2UI CCWGG 2 cut(s) 554, 601
Bst4CI ACNGT 1 cut(s) 87
Bst6I CTCTTC 2 cut(s) 197, 269
BstACI GRCGYC 4 cut(s) 118, 183, 224, 459
BstAPI GCANNNNNTGC 1 cut(s) 444
BstDEI CTNAG 2 cut(s) 255, 277
BstDSI CCRYGG 1 cut(s) 477
BstFNI CGCG 4 cut(s) 215, 239, 302, 304
BstH2I RGCGCY 1 cut(s) 227
BstHHI GCGC 4 cut(s) 135, 226, 241, 304
BstKTI GATC 3 cut(s) 357, 453, 621
BstMAI GTCTC 2 cut(s) 466, 622
BstMBI GATC 3 cut(s) 354, 450, 618
BstMWI GCNNNNNNNGC 4 cut(s) 221, 370, 444, 586
BstNI CCWGG 2 cut(s) 554, 601
BstSCI CCNGG 2 cut(s) 552, 599
BstUI CGCG 4 cut(s) 215, 239, 302, 304
BstV1I GCAGC 2 cut(s) 357, 573
BstV2I GAAGAC 1 cut(s) 190
BsuRI GGCC 3 cut(s) 385, 419, 572
BtgI CCRYGG 1 cut(s) 477
BtrI CACGTC 1 cut(s) 246
BtsIMutI CAGTG 1 cut(s) 386
BveI ACCTGC 1 cut(s) 452
CciI TCATGA 1 cut(s) 324
CfoI GCGC 4 cut(s) 135, 226, 241, 304
CseI GACGC 6 cut(s) 66, 126, 191, 245, 262, 448
Csp6I GTAC 4 cut(s) 298, 310, 406, 541
CviAII CATG 2 cut(s) 325, 478
CviQI GTAC 4 cut(s) 298, 310, 406, 541
DdeI CTNAG 2 cut(s) 255, 277
DinI GGCGCC 1 cut(s) 225
DpnI GATC 3 cut(s) 356, 452, 620
DpnII GATC 3 cut(s) 354, 450, 618
EaeI YGGCCR 1 cut(s) 570
Eam1104I CTCTTC 2 cut(s) 197, 269
EarI CTCTTC 2 cut(s) 197, 269
Ecl136II GAGCTC 1 cut(s) 286
Eco130I CCWWGG 1 cut(s) 477
Eco24I GRGCYC 1 cut(s) 288
Eco31I GGTCTC 1 cut(s) 622
Eco53kI GAGCTC 1 cut(s) 286
Eco88I CYCGRG 1 cut(s) 268
EcoICRI GAGCTC 1 cut(s) 286
EcoRII CCWGG 2 cut(s) 552, 599
EcoT14I CCWWGG 1 cut(s) 477
EcoT38I GRGCYC 1 cut(s) 288
EgeI GGCGCC 1 cut(s) 225
EheI GGCGCC 1 cut(s) 225
ErhI CCWWGG 1 cut(s) 477
Esp3I CGTCTC 1 cut(s) 466
FaeI CATG 2 cut(s) 328, 481
FatI CATG 2 cut(s) 324, 477
FauI CCCGC 1 cut(s) 86
Fnu4HI GCNGC 3 cut(s) 222, 371, 587
FriOI GRGCYC 1 cut(s) 288
Fsp4HI GCNGC 3 cut(s) 222, 371, 587
GlaI GCGC 4 cut(s) 134, 225, 240, 303
GluI GCNGC 3 cut(s) 222, 371, 587
HaeII RGCGCY 1 cut(s) 227
HaeIII GGCC 3 cut(s) 385, 419, 572
HgaI GACGC 6 cut(s) 66, 126, 191, 245, 262, 448
HhaI GCGC 4 cut(s) 135, 226, 241, 304
Hin1I GRCGYC 4 cut(s) 118, 183, 224, 459
Hin1II CATG 2 cut(s) 328, 481
Hin6I GCGC 4 cut(s) 133, 224, 239, 302
HinP1I GCGC 4 cut(s) 133, 224, 239, 302
HindIII AAGCTT 1 cut(s) 173
HinfI GANTC 1 cut(s) 502
HphI GGTGA 2 cut(s) 463, 493
Hpy166II GTNNAC 1 cut(s) 181
Hpy188I TCNGA 2 cut(s) 496, 523
Hpy188III TCNNGA 3 cut(s) 268, 289, 325
Hpy8I GTNNAC 1 cut(s) 181
Hpy99I CGWCG 3 cut(s) 33, 209, 239
HpyAV CCTTC 4 cut(s) 81, 152, 606, 646
HpyCH4III ACNGT 1 cut(s) 87
HpyCH4IV ACGT 1 cut(s) 245
HpyF10VI GCNNNNNNNGC 4 cut(s) 221, 370, 444, 586
HpyF3I CTNAG 2 cut(s) 255, 277
HpySE526I ACGT 1 cut(s) 245
Hsp92I GRCGYC 4 cut(s) 118, 183, 224, 459
Hsp92II CATG 2 cut(s) 328, 481
HspAI GCGC 4 cut(s) 133, 224, 239, 302
KasI GGCGCC 1 cut(s) 223
Kzo9I GATC 3 cut(s) 354, 450, 618
Lsp1109I GCAGC 2 cut(s) 357, 573
LweI GCATC 1 cut(s) 18
MaeII ACGT 1 cut(s) 245
MaeIII GTNAC 1 cut(s) 481
MalI GATC 3 cut(s) 356, 452, 620
MboI GATC 3 cut(s) 354, 450, 618
MboII GAAGA 4 cut(s) 184, 190, 193, 256
MhlI GDGCHC 2 cut(s) 166, 288
MlsI TGGCCA 1 cut(s) 572
MluCI AATT 3 cut(s) 34, 136, 411
MluNI TGGCCA 1 cut(s) 572
Mly113I GGCGCC 1 cut(s) 224
MnlI CCTC 8 cut(s) 85, 161, 200, 272, 348, 396, 430, 484
Mox20I TGGCCA 1 cut(s) 572
MroXI GAANNNNTTC 1 cut(s) 501
MscI TGGCCA 1 cut(s) 572
MseI TTAA 2 cut(s) 170, 230
MslI CAYNNNNRTG 1 cut(s) 476
Msp20I TGGCCA 1 cut(s) 572
MspR9I CCNGG 2 cut(s) 554, 601
MvaI CCWGG 2 cut(s) 554, 601
MvnI CGCG 4 cut(s) 215, 239, 302, 304
MwoI GCNNNNNNNGC 4 cut(s) 221, 370, 444, 586
NarI GGCGCC 1 cut(s) 224
NcoI CCATGG 1 cut(s) 477
NdeII GATC 3 cut(s) 354, 450, 618
NlaIII CATG 2 cut(s) 328, 481
NlaIV GGNNCC 1 cut(s) 225
NmuCI GTSAC 1 cut(s) 481
PagI TCATGA 1 cut(s) 324
PaqCI CACCTGC 1 cut(s) 452
PcsI WCGNNNNNNNCGW 2 cut(s) 201, 354
PdmI GAANNNNTTC 1 cut(s) 501
PfeI GAWTC 1 cut(s) 502
PflFI GACNNNGTC 1 cut(s) 185
PflMI CCANNNNNTGG 1 cut(s) 429
PfoI TCCNGGA 1 cut(s) 599
PkrI GCNGC 3 cut(s) 223, 372, 588
PluTI GGCGCC 1 cut(s) 227
Psp124BI GAGCTC 1 cut(s) 288
Psp6I CCWGG 2 cut(s) 552, 599
PspGI CCWGG 2 cut(s) 552, 599
PspN4I GGNNCC 1 cut(s) 225
PsyI GACNNNGTC 1 cut(s) 185
RsaI GTAC 4 cut(s) 299, 311, 407, 542
RsaNI GTAC 4 cut(s) 298, 310, 406, 541
RseI CAYNNNNRTG 1 cut(s) 476
SacI GAGCTC 1 cut(s) 288
SaqAI TTAA 2 cut(s) 170, 230
SatI GCNGC 3 cut(s) 222, 371, 587
Sau3AI GATC 3 cut(s) 354, 450, 618
ScrFI CCNGG 2 cut(s) 554, 601
SduI GDGCHC 2 cut(s) 166, 288
SfaNI GCATC 1 cut(s) 18
SfoI GGCGCC 1 cut(s) 225
SmiMI CAYNNNNRTG 1 cut(s) 476
SmlI CTYRAG 2 cut(s) 287, 365
SmoI CTYRAG 2 cut(s) 287, 365
Sse9I AATT 3 cut(s) 34, 136, 411
SsiI CCGC 3 cut(s) 79, 215, 221
SspDI GGCGCC 1 cut(s) 223
SstI GAGCTC 1 cut(s) 288
StyD4I CCNGG 2 cut(s) 552, 599
StyI CCWWGG 1 cut(s) 477
TaaI ACNGT 1 cut(s) 87
TaiI ACGT 1 cut(s) 248
TaqI TCGA 3 cut(s) 31, 46, 234
TasI AATT 3 cut(s) 34, 136, 411
TatI WGTACW 1 cut(s) 540
TauI GCSGC 1 cut(s) 224
TfiI GAWTC 1 cut(s) 502
Tru1I TTAA 2 cut(s) 170, 230
Tru9I TTAA 2 cut(s) 170, 230
TscAI CASTG 1 cut(s) 386
TseFI GTSAC 1 cut(s) 481
TseI GCWGC 2 cut(s) 370, 586
Tsp45I GTSAC 1 cut(s) 481
TspDTI ATGAA 3 cut(s) 17, 134, 334
TspGWI ACGGA 1 cut(s) 423
TspRI CASTG 1 cut(s) 386
Tth111I GACNNNGTC 1 cut(s) 185
Van91I CCANNNNNTGG 1 cut(s) 429
XapI RAATTY 1 cut(s) 411
XmnI GAANNNNTTC 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.