pycom09g02970

Protein of unknown function (DUF2781)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0001495
Physical Location & Seq
Reverse (-)
2192030 .. 2196111
4082 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g02970.1

Sequence Viewer

Length: 1035 bp
ATGGGTGTTCTTGTCAAGCTCATTGATGCGTTACTCTTCCTCTTGCTCCTGGCGATCGCTTTCACAACGCCATTGATTGAAGCGCAAGCATGCCTCCCTCGCAATCTCTACCCGGACATTCTCGTGGACCTCAGGAATTGGTACACTCGCGAGTCCGGCGACTATCTCATGACCGAAATGCCTGATTTCTACGTCGGGATCATGTGGGTTGAGCTTGTTTTCCAGTGGCCTCTCGTGTTAGTTAACCTTTATGGTATCTTGGCTGGCAAGCCTTGGTTCAACATTACGTGCTTCAGCTATGGAATATTTCTTTTCACTTCCATGGTGCCAATATTTGCTGAATTGACATGGTCAGGAAGGGCACCTGATAAGCTACTGACGGTGTACTATTGTTTCTTGAGTTTGGGTGTTTTAGCCATACTTCGTGGGCTGCTACCCAACTCATCAACCACGCATACTGCAACCATTGGCAAGAGACCTGCTTCAGGCAGGAAGAAGAGAAGAAAAACACAGCATCAAAATATGGGTGTTTTTGTGAAGACAATCGACGGGTTTCTCTTTGTGAATTTTGCCATGGCGGCGGTGTGCGCGGTGTTGATAGATACACAGTTGTGTCTGCCTCCAAGCCTCTTCCCAAGCTCGGTGGTGGAGATCAAGAACTGGTATGCTTGCGAGTTCGATGATTATCTCGTTGAACAGAAGCCTCATTTCTTGGTTGGCCTCATTTGGGTTGAGCTTACATTGCAGTGGCCTCTTCTCGTTGCCAACTTGTATGCAATTTTGACCGCTAAGCCTTGGTTCAACACCACCTGCTTGGTCTATGGCGTCTCTTTCTTCACTACCATGGGCGCTGTATTAACAGAGATGTTAAGGTCCGGCAAGGCATCTAAGAAAATGTTGAATGTGCACTACGCTGCTATGGGGTTGGCTATTCTGTCCATACTGCGCGGTCTGCTCCCACCGTCCGCTGCCGCTACTCCAACCAGCGGCAAGGGAACAACCAAACGGACCGCCCCACGAAAGAAAATGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2781

Protein Analysis

345

Amino Acids

38.51

Weight (kDa)

9.31

Isoelectric Point (pI)

38.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EBP PF05241 41 - 117 1.5e-10 EXPERA (EXPanded EBP superfamily)
EBP PF05241 217 - 294 7.6e-11 EXPERA (EXPanded EBP superfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 818
Acc36I ACCTGC 2 cut(s) 487, 818
AccB1I GGYRCC 2 cut(s) 325, 361
AccII CGCG 3 cut(s) 150, 590, 948
AciI CCGC 9 cut(s) 578, 581, 590, 786, 948, 966, 972, 987, 1011
AclWI GGATC 1 cut(s) 206
AcsI RAATTY 1 cut(s) 565
AcuI CTGAAG 2 cut(s) 277, 468
AcyI GRCGYC 1 cut(s) 825
AfaI GTAC 2 cut(s) 143, 386
AfiI CCNNNNNNNGG 4 cut(s) 485, 640, 727, 986
AgsI TTSAA 5 cut(s) 80, 280, 695, 802, 901
AjnI CCWGG 1 cut(s) 48
AleI CACNNNNGTG 1 cut(s) 610
AluBI AGCT 6 cut(s) 19, 214, 297, 373, 639, 736
AluI AGCT 6 cut(s) 19, 214, 297, 373, 639, 736
Alw21I GWGCWC 1 cut(s) 909
Alw26I GTCTC 2 cut(s) 469, 832
Alw44I GTGCAC 1 cut(s) 905
AlwI GGATC 1 cut(s) 206
AoxI GGCC 3 cut(s) 227, 718, 749
ApaLI GTGCAC 1 cut(s) 905
ApeKI GCWGC 3 cut(s) 430, 914, 968
ApoI RAATTY 1 cut(s) 565
AsiSI GCGATCGC 1 cut(s) 57
AspLEI GCGC 4 cut(s) 85, 590, 851, 948
AspS9I GGNCC 3 cut(s) 127, 873, 1008
AsuC2I CCSGG 1 cut(s) 113
AvaII GGWCC 3 cut(s) 127, 873, 1008
AxyI CCTNAGG 1 cut(s) 131
BaeGI GKGCMC 2 cut(s) 364, 909
BanI GGYRCC 2 cut(s) 325, 361
BauI CACGAG 2 cut(s) 122, 233
BbsI GAAGAC 1 cut(s) 545
Bbv12I GWGCWC 1 cut(s) 909
BbvI GCAGC 3 cut(s) 417, 901, 955
BciT130I CCWGG 1 cut(s) 50
BcnI CCSGG 1 cut(s) 113
BcoDI GTCTC 2 cut(s) 469, 832
BfoI RGCGCY 1 cut(s) 852
BfuAI ACCTGC 2 cut(s) 487, 818
BglI GCCNNNNNGGC 1 cut(s) 578
BisI GCNGC 6 cut(s) 431, 579, 915, 969, 972, 988
BlpI GCTNAGC 1 cut(s) 789
BlsI GCNGC 6 cut(s) 432, 580, 916, 970, 973, 989
Bme1390I CCNGG 2 cut(s) 50, 113
Bme18I GGWCC 3 cut(s) 127, 873, 1008
BmgT120I GGNCC 3 cut(s) 127, 873, 1008
BmiI GGNNCC 2 cut(s) 327, 363
BmrFI CCNGG 2 cut(s) 50, 113
BmsI GCATC 3 cut(s) 16, 523, 893
BpiI GAAGAC 1 cut(s) 545
Bpu1102I GCTNAGC 1 cut(s) 789
BpuEI CTTGAG 1 cut(s) 418
BpuMI CCSGG 1 cut(s) 113
BsaAI YACGTR 1 cut(s) 288
BsaBI GATNNNNATC 1 cut(s) 684
BsaHI GRCGYC 1 cut(s) 825
BsaI GGTCTC 1 cut(s) 469
BsaJI CCNNGG 5 cut(s) 272, 321, 573, 794, 843
Bsc4I CCNNNNNNNGG 4 cut(s) 485, 640, 727, 986
Bse1I ACTGG 2 cut(s) 223, 665
Bse21I CCTNAGG 1 cut(s) 131
Bse3DI GCAATG 1 cut(s) 740
Bse8I GATNNNNATC 1 cut(s) 684
BseBI CCWGG 1 cut(s) 50
BseDI CCNNGG 5 cut(s) 272, 321, 573, 794, 843
BseJI GATNNNNATC 1 cut(s) 684
BseLI CCNNNNNNNGG 4 cut(s) 485, 640, 727, 986
BseMI GCAATG 1 cut(s) 740
BseMII CTCAG 1 cut(s) 145
BseNI ACTGG 2 cut(s) 223, 665
BseSI GKGCMC 2 cut(s) 364, 909
BseXI GCAGC 3 cut(s) 417, 901, 955
Bsh1236I CGCG 3 cut(s) 150, 590, 948
Bsh1285I CGRYCG 1 cut(s) 57
BshFI GGCC 3 cut(s) 229, 720, 751
BshNI GGYRCC 2 cut(s) 325, 361
BsiEI CGRYCG 1 cut(s) 57
BsiHKAI GWGCWC 1 cut(s) 909
BsiSI CCGG 3 cut(s) 113, 156, 876
BslI CCNNNNNNNGG 4 cut(s) 485, 640, 727, 986
BsmAI GTCTC 2 cut(s) 469, 832
BsmBI CGTCTC 1 cut(s) 832
BsnI GGCC 3 cut(s) 229, 720, 751
Bso31I GGTCTC 1 cut(s) 469
Bsp1286I GDGCHC 2 cut(s) 364, 909
Bsp143I GATC 3 cut(s) 54, 198, 651
Bsp1720I GCTNAGC 1 cut(s) 789
Bsp19I CCATGG 3 cut(s) 321, 573, 843
Bsp68I TCGCGA 1 cut(s) 150
BspACI CCGC 9 cut(s) 578, 581, 590, 786, 948, 966, 972, 987, 1011
BspANI GGCC 3 cut(s) 229, 720, 751
BspCNI CTCAG 1 cut(s) 144
BspFNI CGCG 3 cut(s) 150, 590, 948
BspHI TCATGA 1 cut(s) 168
BspLI GGNNCC 2 cut(s) 327, 363
BspMI ACCTGC 2 cut(s) 487, 818
BspPI GGATC 1 cut(s) 206
BspT107I GGYRCC 2 cut(s) 325, 361
BspTNI GGTCTC 1 cut(s) 469
BsrDI GCAATG 1 cut(s) 740
BsrI ACTGG 2 cut(s) 223, 665
BssECI CCNNGG 5 cut(s) 272, 321, 573, 794, 843
BssMI GATC 3 cut(s) 54, 198, 651
BssNI GRCGYC 1 cut(s) 825
BssSI CACGAG 2 cut(s) 122, 233
BssT1I CCWWGG 5 cut(s) 272, 321, 573, 794, 843
Bst2BI CACGAG 2 cut(s) 122, 233
Bst2UI CCWGG 1 cut(s) 50
Bst4CI ACNGT 3 cut(s) 382, 609, 963
Bst6I CTCTTC 4 cut(s) 41, 491, 635, 759
BstACI GRCGYC 1 cut(s) 825
BstBAI YACGTR 1 cut(s) 288
BstC8I GCNNGC 5 cut(s) 87, 91, 265, 269, 670
BstDEI CTNAG 3 cut(s) 131, 789, 888
BstDSI CCRYGG 3 cut(s) 321, 573, 843
BstENI CCTNNNNNAGG 1 cut(s) 483
BstFNI CGCG 3 cut(s) 150, 590, 948
BstH2I RGCGCY 1 cut(s) 852
BstHHI GCGC 4 cut(s) 85, 590, 851, 948
BstKTI GATC 3 cut(s) 57, 201, 654
BstMAI GTCTC 2 cut(s) 469, 832
BstMBI GATC 3 cut(s) 54, 198, 651
BstMCI CGRYCG 1 cut(s) 57
BstMWI GCNNNNNNNGC 6 cut(s) 99, 156, 578, 587, 742, 952
BstNI CCWGG 1 cut(s) 50
BstNSI RCATGY 1 cut(s) 93
BstSCI CCNGG 2 cut(s) 48, 111
BstSLI GKGCMC 2 cut(s) 364, 909
BstUI CGCG 3 cut(s) 150, 590, 948
BstV1I GCAGC 3 cut(s) 417, 901, 955
BstV2I GAAGAC 1 cut(s) 545
BstXI CCANNNNNNTGG 1 cut(s) 814
Bsu36I CCTNAGG 1 cut(s) 131
BsuRI GGCC 3 cut(s) 229, 720, 751
BtgI CCRYGG 3 cut(s) 321, 573, 843
BtsI GCAGTG 1 cut(s) 752
BtsIMutI CAGTG 2 cut(s) 230, 752
BtuMI TCGCGA 1 cut(s) 150
BveI ACCTGC 2 cut(s) 487, 818
Cac8I GCNNGC 5 cut(s) 87, 91, 265, 269, 670
CciI TCATGA 1 cut(s) 168
CfoI GCGC 4 cut(s) 85, 590, 851, 948
Cfr13I GGNCC 3 cut(s) 127, 873, 1008
CpoI CGGWCCG 1 cut(s) 1008
CseI GACGC 1 cut(s) 814
Csp6I GTAC 2 cut(s) 142, 385
CspCI CAANNNNNGTGG 2 cut(s) 624, 659
CspI CGGWCCG 1 cut(s) 1008
CviAII CATG 7 cut(s) 90, 169, 202, 322, 348, 574, 844
CviQI GTAC 2 cut(s) 142, 385
DdeI CTNAG 3 cut(s) 131, 789, 888
DpnI GATC 3 cut(s) 56, 200, 653
DpnII GATC 3 cut(s) 54, 198, 651
Eam1104I CTCTTC 4 cut(s) 41, 491, 635, 759
EarI CTCTTC 4 cut(s) 41, 491, 635, 759
Eco130I CCWWGG 5 cut(s) 272, 321, 573, 794, 843
Eco31I GGTCTC 1 cut(s) 469
Eco47I GGWCC 3 cut(s) 127, 873, 1008
Eco57I CTGAAG 2 cut(s) 277, 468
Eco81I CCTNAGG 1 cut(s) 131
EcoNI CCTNNNNNAGG 1 cut(s) 483
EcoRII CCWGG 1 cut(s) 48
EcoT14I CCWWGG 5 cut(s) 272, 321, 573, 794, 843
ErhI CCWWGG 5 cut(s) 272, 321, 573, 794, 843
Esp3I CGTCTC 1 cut(s) 832
FaeI CATG 7 cut(s) 93, 172, 205, 325, 351, 577, 847
FatI CATG 7 cut(s) 89, 168, 201, 321, 347, 573, 843
Fnu4HI GCNGC 6 cut(s) 431, 579, 915, 969, 972, 988
Fsp4HI GCNGC 6 cut(s) 431, 579, 915, 969, 972, 988
GlaI GCGC 4 cut(s) 84, 589, 850, 947
GluI GCNGC 6 cut(s) 431, 579, 915, 969, 972, 988
HaeII RGCGCY 1 cut(s) 852
HaeIII GGCC 3 cut(s) 229, 720, 751
HapII CCGG 3 cut(s) 113, 156, 876
HgaI GACGC 1 cut(s) 814
HhaI GCGC 4 cut(s) 85, 590, 851, 948
Hin1I GRCGYC 1 cut(s) 825
Hin1II CATG 7 cut(s) 93, 172, 205, 325, 351, 577, 847
Hin6I GCGC 4 cut(s) 83, 588, 849, 946
HinP1I GCGC 4 cut(s) 83, 588, 849, 946
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HinfI GANTC 1 cut(s) 152
HpaI GTTAAC 1 cut(s) 244
HpaII CCGG 3 cut(s) 113, 156, 876
Hpy166II GTNNAC 5 cut(s) 127, 144, 244, 385, 907
Hpy188III TCNNGA 7 cut(s) 133, 149, 169, 196, 354, 397, 655
Hpy8I GTNNAC 5 cut(s) 127, 144, 244, 385, 907
Hpy99I CGWCG 2 cut(s) 197, 551
HpyAV CCTTC 1 cut(s) 351
HpyCH4III ACNGT 3 cut(s) 382, 609, 963
HpyCH4IV ACGT 2 cut(s) 192, 287
HpyCH4V TGCA 4 cut(s) 461, 745, 776, 907
HpyF10VI GCNNNNNNNGC 6 cut(s) 99, 156, 578, 587, 742, 952
HpyF3I CTNAG 3 cut(s) 131, 789, 888
HpySE526I ACGT 2 cut(s) 192, 287
Hsp92I GRCGYC 1 cut(s) 825
Hsp92II CATG 7 cut(s) 93, 172, 205, 325, 351, 577, 847
HspAI GCGC 4 cut(s) 83, 588, 849, 946
KspAI GTTAAC 1 cut(s) 244
Kzo9I GATC 3 cut(s) 54, 198, 651
LmnI GCTCC 2 cut(s) 51, 960
Lsp1109I GCAGC 3 cut(s) 417, 901, 955
LweI GCATC 3 cut(s) 16, 523, 893
MaeII ACGT 2 cut(s) 192, 287
MaeIII GTNAC 1 cut(s) 30
MalI GATC 3 cut(s) 56, 200, 653
MboI GATC 3 cut(s) 54, 198, 651
MboII GAAGA 8 cut(s) 28, 505, 508, 513, 550, 622, 746, 826
MhlI GDGCHC 2 cut(s) 364, 909
MluCI AATT 4 cut(s) 136, 341, 565, 777
MlyI GAGTC 1 cut(s) 161
MmeI TCCRAC 1 cut(s) 1004
MseI TTAA 3 cut(s) 243, 857, 869
MslI CAYNNNNRTG 5 cut(s) 122, 320, 610, 745, 842
MspA1I CMGCKG 2 cut(s) 968, 987
MspI CCGG 3 cut(s) 113, 156, 876
MspR9I CCNGG 2 cut(s) 50, 113
MvaI CCWGG 1 cut(s) 50
MvnI CGCG 3 cut(s) 150, 590, 948
MwoI GCNNNNNNNGC 6 cut(s) 99, 156, 578, 587, 742, 952
NciI CCSGG 1 cut(s) 113
NcoI CCATGG 3 cut(s) 321, 573, 843
NdeII GATC 3 cut(s) 54, 198, 651
NlaIII CATG 7 cut(s) 93, 172, 205, 325, 351, 577, 847
NlaIV GGNNCC 2 cut(s) 327, 363
NruI TCGCGA 1 cut(s) 150
NspI RCATGY 1 cut(s) 93
OliI CACNNNNGTG 1 cut(s) 610
PaeI GCATGC 1 cut(s) 93
PagI TCATGA 1 cut(s) 168
PaqCI CACCTGC 1 cut(s) 818
PflFI GACNNNGTC 1 cut(s) 349
PkrI GCNGC 6 cut(s) 432, 580, 916, 970, 973, 989
Ple19I CGATCG 1 cut(s) 57
PleI GAGTC 1 cut(s) 160
PpsI GAGTC 1 cut(s) 160
Ppu21I YACGTR 1 cut(s) 288
Psp6I CCWGG 1 cut(s) 48
PspGI CCWGG 1 cut(s) 48
PspN4I GGNNCC 2 cut(s) 327, 363
PspPI GGNCC 3 cut(s) 127, 873, 1008
PsyI GACNNNGTC 1 cut(s) 349
PvuI CGATCG 1 cut(s) 57
RgaI GCGATCGC 1 cut(s) 57
RruI TCGCGA 1 cut(s) 150
RsaI GTAC 2 cut(s) 143, 386
RsaNI GTAC 2 cut(s) 142, 385
RseI CAYNNNNRTG 5 cut(s) 122, 320, 610, 745, 842
Rsr2I CGGWCCG 1 cut(s) 1008
RsrII CGGWCCG 1 cut(s) 1008
SaqAI TTAA 3 cut(s) 243, 857, 869
SatI GCNGC 6 cut(s) 431, 579, 915, 969, 972, 988
Sau3AI GATC 3 cut(s) 54, 198, 651
Sau96I GGNCC 3 cut(s) 127, 873, 1008
SchI GAGTC 1 cut(s) 161
ScrFI CCNGG 2 cut(s) 50, 113
SduI GDGCHC 2 cut(s) 364, 909
SfaAI GCGATCGC 1 cut(s) 57
SfaNI GCATC 3 cut(s) 16, 523, 893
SgfI GCGATCGC 1 cut(s) 57
SinI GGWCC 3 cut(s) 127, 873, 1008
SmiMI CAYNNNNRTG 5 cut(s) 122, 320, 610, 745, 842
SmlI CTYRAG 1 cut(s) 397
SmoI CTYRAG 1 cut(s) 397
SphI GCATGC 1 cut(s) 93
Sse9I AATT 4 cut(s) 136, 341, 565, 777
SsiI CCGC 9 cut(s) 578, 581, 590, 786, 948, 966, 972, 987, 1011
SspI AATATT 2 cut(s) 306, 333
StyD4I CCNGG 2 cut(s) 48, 111
StyI CCWWGG 5 cut(s) 272, 321, 573, 794, 843
TaaI ACNGT 3 cut(s) 382, 609, 963
TaiI ACGT 2 cut(s) 195, 290
TaqI TCGA 2 cut(s) 546, 678
TaqII GACCGA 1 cut(s) 188
TasI AATT 4 cut(s) 136, 341, 565, 777
TatI WGTACW 1 cut(s) 384
TauI GCSGC 3 cut(s) 581, 974, 990
Tru1I TTAA 3 cut(s) 243, 857, 869
Tru9I TTAA 3 cut(s) 243, 857, 869
TscAI CASTG 2 cut(s) 230, 752
TseI GCWGC 3 cut(s) 430, 914, 968
TspGWI ACGGA 1 cut(s) 1021
TspRI CASTG 2 cut(s) 230, 752
Tth111I GACNNNGTC 1 cut(s) 349
VneI GTGCAC 1 cut(s) 905
VpaK11BI GGWCC 3 cut(s) 127, 873, 1008
XagI CCTNNNNNAGG 1 cut(s) 483
XapI RAATTY 1 cut(s) 565
XceI RCATGY 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.