Rh2CG527900

Transmembrane protein 97-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0001495
Physical Location & Seq
Forward (+)
70297762 .. 70300480
2719 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG527900.1

Sequence Viewer

Length: 504 bp
ATGGGTGCTCTGTTAAAGCTTGTGGACGCCGTCCTCTTCGTCTTCTTCGTCGTAATCGCGGTGGCGGCGCCGTTAATCGACGCGCAGACGTGTCTGCCTGAGAGCCTCTTCCCGAGCGTCTTAGTTGAGCTCAAGAACTGGTACGCGCGTGAGTACGGCGACTATCTCATGACCCAGAAGCCTCATTTCATCGTTGGGATCGTCTGGCTTGAGCTGCTGTTTCAGTGGCCTCTTGCTCTTGCCAACCTGTACGGAATTTTGGCCTCCAAGTCTTGGTTTAGCACCACCTGCTTGATCTTTGGCGTCTCTGTTTTCACCTCCATGGTGACTATATTATCAGAACTGATTCAGTCCGGCAAGGCTTCTGATAAGCTGTTGTTTATGTACTTCCCTTTCCTGGGGTTGGGTGTTTTGGCCATACTGCGTGGGCTGCTGACACCTTCCAGGACGAGTAGTTCAATGATCGGCAAGAGACCTATGTTGGGCAGGAAAAAGAAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2781

Protein Analysis

167

Amino Acids

18.54

Weight (kDa)

9.48

Isoelectric Point (pI)

28.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EBP PF05241 41 - 140 3.1e-14 EXPERA (EXPanded EBP superfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 296
Acc36I ACCTGC 1 cut(s) 296
AccB1I GGYRCC 1 cut(s) 67
AccB7I CCANNNNNTGG 1 cut(s) 273
AccII CGCG 4 cut(s) 59, 83, 146, 148
AciI CCGC 2 cut(s) 59, 65
AclWI GGATC 1 cut(s) 206
AcoI YGGCCR 1 cut(s) 414
AcsI RAATTY 1 cut(s) 255
AcyI GRCGYC 3 cut(s) 27, 68, 303
AfaI GTAC 4 cut(s) 143, 155, 251, 386
AfiI CCNNNNNNNGG 5 cut(s) 273, 397, 398, 403, 482
AflIII ACRYGT 1 cut(s) 89
AgsI TTSAA 1 cut(s) 459
AjiI CACGTC 1 cut(s) 90
AjnI CCWGG 2 cut(s) 396, 443
AloI GAACNNNNNNTCC 2 cut(s) 439, 471
AluBI AGCT 4 cut(s) 19, 130, 214, 373
AluI AGCT 4 cut(s) 19, 130, 214, 373
Alw21I GWGCWC 2 cut(s) 10, 132
Alw26I GTCTC 2 cut(s) 310, 466
AlwI GGATC 1 cut(s) 206
Ama87I CYCGRG 1 cut(s) 112
AoxI GGCC 3 cut(s) 227, 261, 414
ApeKI GCWGC 2 cut(s) 214, 430
ApoI RAATTY 1 cut(s) 255
Asp700I GAANNNNTTC 1 cut(s) 345
AspLEI GCGC 3 cut(s) 70, 85, 148
AsuHPI GGTGA 2 cut(s) 307, 337
AvaI CYCGRG 1 cut(s) 112
BaeI ACNNNNGTAYC 2 cut(s) 133, 166
BalI TGGCCA 1 cut(s) 416
BanI GGYRCC 1 cut(s) 67
BanII GRGCYC 1 cut(s) 132
BbsI GAAGAC 1 cut(s) 34
Bbv12I GWGCWC 2 cut(s) 10, 132
BbvI GCAGC 2 cut(s) 201, 417
BceAI ACGGC 3 cut(s) 14, 55, 172
BciT130I CCWGG 2 cut(s) 398, 445
BcoDI GTCTC 2 cut(s) 310, 466
BfoI RGCGCY 1 cut(s) 71
BfuAI ACCTGC 1 cut(s) 296
BisI GCNGC 3 cut(s) 66, 215, 431
BlsI GCNGC 3 cut(s) 67, 216, 432
Bme1390I CCNGG 2 cut(s) 398, 445
BmeT110I CYCGRG 1 cut(s) 112
BmgBI CACGTC 1 cut(s) 90
BmiI GGNNCC 1 cut(s) 69
BmrFI CCNGG 2 cut(s) 398, 445
BpiI GAAGAC 1 cut(s) 34
BpuEI CTTGAG 2 cut(s) 116, 230
BsaHI GRCGYC 3 cut(s) 27, 68, 303
BsaI GGTCTC 1 cut(s) 466
BsaJI CCNNGG 2 cut(s) 321, 397
Bsc4I CCNNNNNNNGG 5 cut(s) 273, 397, 398, 403, 482
Bse1I ACTGG 1 cut(s) 143
BseBI CCWGG 2 cut(s) 398, 445
BseDI CCNNGG 2 cut(s) 321, 397
BseLI CCNNNNNNNGG 5 cut(s) 273, 397, 398, 403, 482
BseMII CTCAG 1 cut(s) 90
BseNI ACTGG 1 cut(s) 143
BseXI GCAGC 2 cut(s) 201, 417
Bsh1236I CGCG 4 cut(s) 59, 83, 146, 148
BshFI GGCC 3 cut(s) 229, 263, 416
BshNI GGYRCC 1 cut(s) 67
BsiHKAI GWGCWC 2 cut(s) 10, 132
BsiHKCI CYCGRG 1 cut(s) 112
BsiSI CCGG 1 cut(s) 354
BslI CCNNNNNNNGG 5 cut(s) 273, 397, 398, 403, 482
BsmAI GTCTC 2 cut(s) 310, 466
BsmBI CGTCTC 1 cut(s) 310
BsnI GGCC 3 cut(s) 229, 263, 416
Bso31I GGTCTC 1 cut(s) 466
BsoBI CYCGRG 1 cut(s) 112
Bsp1286I GDGCHC 2 cut(s) 10, 132
Bsp143I GATC 3 cut(s) 198, 294, 462
Bsp19I CCATGG 1 cut(s) 321
BspACI CCGC 2 cut(s) 59, 65
BspANI GGCC 3 cut(s) 229, 263, 416
BspCNI CTCAG 1 cut(s) 91
BspFNI CGCG 4 cut(s) 59, 83, 146, 148
BspHI TCATGA 1 cut(s) 168
BspLI GGNNCC 1 cut(s) 69
BspMI ACCTGC 1 cut(s) 296
BspPI GGATC 1 cut(s) 206
BspT107I GGYRCC 1 cut(s) 67
BspTNI GGTCTC 1 cut(s) 466
BsrI ACTGG 1 cut(s) 143
BssECI CCNNGG 2 cut(s) 321, 397
BssMI GATC 3 cut(s) 198, 294, 462
BssNI GRCGYC 3 cut(s) 27, 68, 303
BssT1I CCWWGG 1 cut(s) 321
Bst2UI CCWGG 2 cut(s) 398, 445
Bst6I CTCTTC 2 cut(s) 41, 113
BstACI GRCGYC 3 cut(s) 27, 68, 303
BstAPI GCANNNNNTGC 1 cut(s) 288
BstDEI CTNAG 2 cut(s) 99, 121
BstDSI CCRYGG 1 cut(s) 321
BstFNI CGCG 4 cut(s) 59, 83, 146, 148
BstH2I RGCGCY 1 cut(s) 71
BstHHI GCGC 3 cut(s) 70, 85, 148
BstKTI GATC 3 cut(s) 201, 297, 465
BstMAI GTCTC 2 cut(s) 310, 466
BstMBI GATC 3 cut(s) 198, 294, 462
BstMWI GCNNNNNNNGC 4 cut(s) 65, 214, 288, 430
BstNI CCWGG 2 cut(s) 398, 445
BstSCI CCNGG 2 cut(s) 396, 443
BstUI CGCG 4 cut(s) 59, 83, 146, 148
BstV1I GCAGC 2 cut(s) 201, 417
BstV2I GAAGAC 1 cut(s) 34
BsuRI GGCC 3 cut(s) 229, 263, 416
BtgI CCRYGG 1 cut(s) 321
BtrI CACGTC 1 cut(s) 90
BtsIMutI CAGTG 1 cut(s) 230
BveI ACCTGC 1 cut(s) 296
CciI TCATGA 1 cut(s) 168
CfoI GCGC 3 cut(s) 70, 85, 148
CseI GACGC 4 cut(s) 35, 89, 106, 292
Csp6I GTAC 4 cut(s) 142, 154, 250, 385
CviAII CATG 2 cut(s) 169, 322
CviQI GTAC 4 cut(s) 142, 154, 250, 385
DdeI CTNAG 2 cut(s) 99, 121
DinI GGCGCC 1 cut(s) 69
DpnI GATC 3 cut(s) 200, 296, 464
DpnII GATC 3 cut(s) 198, 294, 462
EaeI YGGCCR 1 cut(s) 414
Eam1104I CTCTTC 2 cut(s) 41, 113
EarI CTCTTC 2 cut(s) 41, 113
Ecl136II GAGCTC 1 cut(s) 130
Eco130I CCWWGG 1 cut(s) 321
Eco24I GRGCYC 1 cut(s) 132
Eco31I GGTCTC 1 cut(s) 466
Eco53kI GAGCTC 1 cut(s) 130
Eco88I CYCGRG 1 cut(s) 112
EcoICRI GAGCTC 1 cut(s) 130
EcoRII CCWGG 2 cut(s) 396, 443
EcoT14I CCWWGG 1 cut(s) 321
EcoT38I GRGCYC 1 cut(s) 132
EgeI GGCGCC 1 cut(s) 69
EheI GGCGCC 1 cut(s) 69
ErhI CCWWGG 1 cut(s) 321
Esp3I CGTCTC 1 cut(s) 310
FaeI CATG 2 cut(s) 172, 325
FaiI YATR 6 cut(s) 170, 323, 332, 383, 419, 479
FatI CATG 2 cut(s) 168, 321
Fnu4HI GCNGC 3 cut(s) 66, 215, 431
FriOI GRGCYC 1 cut(s) 132
Fsp4HI GCNGC 3 cut(s) 66, 215, 431
GlaI GCGC 3 cut(s) 69, 84, 147
GluI GCNGC 3 cut(s) 66, 215, 431
HaeII RGCGCY 1 cut(s) 71
HaeIII GGCC 3 cut(s) 229, 263, 416
HapII CCGG 1 cut(s) 354
HgaI GACGC 4 cut(s) 35, 89, 106, 292
HhaI GCGC 3 cut(s) 70, 85, 148
Hin1I GRCGYC 3 cut(s) 27, 68, 303
Hin1II CATG 2 cut(s) 172, 325
Hin6I GCGC 3 cut(s) 68, 83, 146
HinP1I GCGC 3 cut(s) 68, 83, 146
HindIII AAGCTT 1 cut(s) 17
HinfI GANTC 1 cut(s) 346
HpaII CCGG 1 cut(s) 354
HphI GGTGA 2 cut(s) 307, 337
Hpy166II GTNNAC 1 cut(s) 25
Hpy188I TCNGA 2 cut(s) 340, 367
Hpy188III TCNNGA 3 cut(s) 112, 133, 169
Hpy8I GTNNAC 1 cut(s) 25
Hpy99I CGWCG 2 cut(s) 53, 83
HpyAV CCTTC 2 cut(s) 450, 490
HpyCH4IV ACGT 1 cut(s) 89
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 214, 288, 430
HpyF3I CTNAG 2 cut(s) 99, 121
HpySE526I ACGT 1 cut(s) 89
Hsp92I GRCGYC 3 cut(s) 27, 68, 303
Hsp92II CATG 2 cut(s) 172, 325
HspAI GCGC 3 cut(s) 68, 83, 146
KasI GGCGCC 1 cut(s) 67
Kzo9I GATC 3 cut(s) 198, 294, 462
Lsp1109I GCAGC 2 cut(s) 201, 417
MaeII ACGT 1 cut(s) 89
MaeIII GTNAC 1 cut(s) 325
MalI GATC 3 cut(s) 200, 296, 464
MboI GATC 3 cut(s) 198, 294, 462
MboII GAAGA 4 cut(s) 28, 34, 37, 100
MhlI GDGCHC 2 cut(s) 10, 132
MlsI TGGCCA 1 cut(s) 416
MluCI AATT 1 cut(s) 255
MluNI TGGCCA 1 cut(s) 416
Mly113I GGCGCC 1 cut(s) 68
MnlI CCTC 6 cut(s) 44, 116, 192, 240, 274, 328
Mox20I TGGCCA 1 cut(s) 416
MroXI GAANNNNTTC 1 cut(s) 345
MscI TGGCCA 1 cut(s) 416
MseI TTAA 2 cut(s) 14, 74
MslI CAYNNNNRTG 1 cut(s) 320
Msp20I TGGCCA 1 cut(s) 416
MspI CCGG 1 cut(s) 354
MspR9I CCNGG 2 cut(s) 398, 445
MvaI CCWGG 2 cut(s) 398, 445
MvnI CGCG 4 cut(s) 59, 83, 146, 148
MwoI GCNNNNNNNGC 4 cut(s) 65, 214, 288, 430
NarI GGCGCC 1 cut(s) 68
NcoI CCATGG 1 cut(s) 321
NdeII GATC 3 cut(s) 198, 294, 462
NlaIII CATG 2 cut(s) 172, 325
NlaIV GGNNCC 1 cut(s) 69
NmuCI GTSAC 1 cut(s) 325
PagI TCATGA 1 cut(s) 168
PaqCI CACCTGC 1 cut(s) 296
PcsI WCGNNNNNNNCGW 2 cut(s) 45, 198
PdmI GAANNNNTTC 1 cut(s) 345
PfeI GAWTC 1 cut(s) 346
PflFI GACNNNGTC 1 cut(s) 29
PflMI CCANNNNNTGG 1 cut(s) 273
PfoI TCCNGGA 1 cut(s) 443
PkrI GCNGC 3 cut(s) 67, 216, 432
PluTI GGCGCC 1 cut(s) 71
Psp124BI GAGCTC 1 cut(s) 132
Psp6I CCWGG 2 cut(s) 396, 443
PspGI CCWGG 2 cut(s) 396, 443
PspN4I GGNNCC 1 cut(s) 69
PsyI GACNNNGTC 1 cut(s) 29
RsaI GTAC 4 cut(s) 143, 155, 251, 386
RsaNI GTAC 4 cut(s) 142, 154, 250, 385
RseI CAYNNNNRTG 1 cut(s) 320
SacI GAGCTC 1 cut(s) 132
SaqAI TTAA 2 cut(s) 14, 74
SatI GCNGC 3 cut(s) 66, 215, 431
Sau3AI GATC 3 cut(s) 198, 294, 462
ScrFI CCNGG 2 cut(s) 398, 445
SduI GDGCHC 2 cut(s) 10, 132
SfoI GGCGCC 1 cut(s) 69
SmiMI CAYNNNNRTG 1 cut(s) 320
SmlI CTYRAG 2 cut(s) 131, 209
SmoI CTYRAG 2 cut(s) 131, 209
Sse9I AATT 1 cut(s) 255
SsiI CCGC 2 cut(s) 59, 65
SspDI GGCGCC 1 cut(s) 67
SstI GAGCTC 1 cut(s) 132
StyD4I CCNGG 2 cut(s) 396, 443
StyI CCWWGG 1 cut(s) 321
TaiI ACGT 1 cut(s) 92
TaqI TCGA 1 cut(s) 78
TasI AATT 1 cut(s) 255
TatI WGTACW 1 cut(s) 384
TauI GCSGC 1 cut(s) 68
TfiI GAWTC 1 cut(s) 346
Tru1I TTAA 2 cut(s) 14, 74
Tru9I TTAA 2 cut(s) 14, 74
TscAI CASTG 1 cut(s) 230
TseFI GTSAC 1 cut(s) 325
TseI GCWGC 2 cut(s) 214, 430
Tsp45I GTSAC 1 cut(s) 325
TspDTI ATGAA 1 cut(s) 178
TspGWI ACGGA 1 cut(s) 267
TspRI CASTG 1 cut(s) 230
Tth111I GACNNNGTC 1 cut(s) 29
Van91I CCANNNNNTGG 1 cut(s) 273
XapI RAATTY 1 cut(s) 255
XmnI GAANNNNTTC 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.