RLG00000021171

Protein of unknown function (DUF2781)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001495
Physical Location & Seq
Forward (+)
72575126 .. 72576732
1607 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021171

Sequence Viewer

Length: 513 bp
ATGGGGTCTCTTGTTAAGCTCATTGATGCTTTGCTCTTCCTCCTGCTCCTCGCGATCGCTTTTGAAACCCCATTGATTGAAGGGCAAGCATGTCTCCCTATCACCCTCTACCCAGATTTTCTCGTCGAACTCCGGAGCTGGTACGCAGGAGAAGTTGGTGACTACCTCATGACTGAGACGCCGGATTTCTACGTCGGGATCATATGGGTTGAGCTTCTTTTTCAATGGCCTCTGGTGCTTGTGAATCTTTATGGCATTTTAGCTGGGAAGCATTGGTTCAACACGACGTGCTTGAGTTATGGCATTTCTCTATTCACTGGCATGGTGCCTATATTTTCAGAACTCACAAGATCAGGAAGGGCTCCGGAAAAGTTATTGACGGTGTACTATTCGTTCTTGAGTGTGGGTGTTGTAGCCATACTTCGTGGATTGCTTCCCAACTCATCATCGACCAAGTTCTCTTCTGCAACCATTGGCAAGAGACCCGCCTCCGGTAGGAAGAAGAGAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2781

Protein Analysis

171

Amino Acids

18.91

Weight (kDa)

7.73

Isoelectric Point (pI)

38.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EBP PF05241 40 - 117 5.5e-11 EXPERA (EXPanded EBP superfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 325
AccII CGCG 1 cut(s) 53
AccIII TCCGGA 2 cut(s) 132, 364
AciI CCGC 1 cut(s) 486
AclWI GGATC 1 cut(s) 206
AcyI GRCGYC 1 cut(s) 179
AdeI CACNNNGTG 1 cut(s) 288
AfaI GTAC 2 cut(s) 143, 386
AfiI CCNNNNNNNGG 2 cut(s) 491, 495
AgsI TTSAA 4 cut(s) 65, 80, 224, 280
AjiI CACGTC 1 cut(s) 288
AluBI AGCT 4 cut(s) 19, 138, 214, 263
AluI AGCT 4 cut(s) 19, 138, 214, 263
Alw26I GTCTC 4 cut(s) 12, 98, 170, 475
AlwI GGATC 1 cut(s) 206
Aor13HI TCCGGA 2 cut(s) 132, 364
AoxI GGCC 1 cut(s) 227
AsiSI GCGATCGC 1 cut(s) 57
AsuHPI GGTGA 2 cut(s) 94, 170
BanI GGYRCC 1 cut(s) 325
BanII GRGCYC 1 cut(s) 364
BarI GAAGNNNNNNTAC 2 cut(s) 405, 437
BcoDI GTCTC 4 cut(s) 12, 98, 170, 475
BmgBI CACGTC 1 cut(s) 288
BmiI GGNNCC 2 cut(s) 327, 363
BmsI GCATC 1 cut(s) 16
BpuEI CTTGAG 2 cut(s) 313, 418
BsaHI GRCGYC 1 cut(s) 179
BsaI GGTCTC 2 cut(s) 12, 475
BsaWI WCCGGW 3 cut(s) 132, 364, 491
Bsc4I CCNNNNNNNGG 2 cut(s) 491, 495
Bse1I ACTGG 1 cut(s) 322
BseAI TCCGGA 2 cut(s) 132, 364
BseLI CCNNNNNNNGG 2 cut(s) 491, 495
BseMII CTCAG 1 cut(s) 165
BseNI ACTGG 1 cut(s) 322
BseRI GAGGAG 1 cut(s) 38
BseYI CCCAGC 1 cut(s) 263
Bsh1236I CGCG 1 cut(s) 53
Bsh1285I CGRYCG 1 cut(s) 57
BshFI GGCC 1 cut(s) 229
BshNI GGYRCC 1 cut(s) 325
BsiEI CGRYCG 1 cut(s) 57
BsiSI CCGG 4 cut(s) 133, 182, 365, 492
BslI CCNNNNNNNGG 2 cut(s) 491, 495
BsmAI GTCTC 4 cut(s) 12, 98, 170, 475
BsmBI CGTCTC 1 cut(s) 170
BsnI GGCC 1 cut(s) 229
Bso31I GGTCTC 2 cut(s) 12, 475
Bsp1286I GDGCHC 1 cut(s) 364
Bsp13I TCCGGA 2 cut(s) 132, 364
Bsp143I GATC 3 cut(s) 54, 198, 350
Bsp68I TCGCGA 1 cut(s) 53
BspACI CCGC 1 cut(s) 486
BspANI GGCC 1 cut(s) 229
BspCNI CTCAG 1 cut(s) 166
BspEI TCCGGA 2 cut(s) 132, 364
BspFNI CGCG 1 cut(s) 53
BspHI TCATGA 1 cut(s) 168
BspLI GGNNCC 2 cut(s) 327, 363
BspPI GGATC 1 cut(s) 206
BspQI GCTCTTC 1 cut(s) 41
BspT107I GGYRCC 1 cut(s) 325
BspTNI GGTCTC 2 cut(s) 12, 475
BsrI ACTGG 1 cut(s) 322
BssMI GATC 3 cut(s) 54, 198, 350
BssNI GRCGYC 1 cut(s) 179
Bst4CI ACNGT 1 cut(s) 382
Bst6I CTCTTC 3 cut(s) 41, 466, 497
BstACI GRCGYC 1 cut(s) 179
BstC8I GCNNGC 1 cut(s) 87
BstDEI CTNAG 1 cut(s) 174
BstENI CCTNNNNNAGG 1 cut(s) 493
BstFNI CGCG 1 cut(s) 53
BstKTI GATC 3 cut(s) 57, 201, 353
BstMAI GTCTC 4 cut(s) 12, 98, 170, 475
BstMBI GATC 3 cut(s) 54, 198, 350
BstMCI CGRYCG 1 cut(s) 57
BstMWI GCNNNNNNNGC 1 cut(s) 235
BstNSI RCATGY 1 cut(s) 93
BstUI CGCG 1 cut(s) 53
BsuRI GGCC 1 cut(s) 229
BtrI CACGTC 1 cut(s) 288
BtsIMutI CAGTG 1 cut(s) 315
BtuMI TCGCGA 1 cut(s) 53
Cac8I GCNNGC 1 cut(s) 87
CciI TCATGA 1 cut(s) 168
CseI GACGC 1 cut(s) 187
Csp6I GTAC 2 cut(s) 142, 385
CviAII CATG 3 cut(s) 90, 169, 322
CviJI RGCY 7 cut(s) 19, 138, 214, 229, 263, 362, 416
CviKI_1 RGCY 7 cut(s) 19, 138, 214, 229, 263, 362, 416
CviQI GTAC 2 cut(s) 142, 385
DdeI CTNAG 1 cut(s) 174
DpnI GATC 3 cut(s) 56, 200, 352
DpnII GATC 3 cut(s) 54, 198, 350
DraIII CACNNNGTG 1 cut(s) 288
Eam1104I CTCTTC 3 cut(s) 41, 466, 497
EarI CTCTTC 3 cut(s) 41, 466, 497
Eco24I GRGCYC 1 cut(s) 364
Eco31I GGTCTC 2 cut(s) 12, 475
EcoNI CCTNNNNNAGG 1 cut(s) 493
EcoT38I GRGCYC 1 cut(s) 364
Esp3I CGTCTC 1 cut(s) 170
FaeI CATG 3 cut(s) 93, 172, 325
FaiI YATR 9 cut(s) 91, 170, 203, 205, 252, 300, 323, 332, 419
FatI CATG 3 cut(s) 89, 168, 321
FauI CCCGC 1 cut(s) 493
FauNDI CATATG 1 cut(s) 203
FriOI GRGCYC 1 cut(s) 364
GsaI CCCAGC 1 cut(s) 267
HaeIII GGCC 1 cut(s) 229
HapII CCGG 4 cut(s) 133, 182, 365, 492
HgaI GACGC 1 cut(s) 187
Hin1I GRCGYC 1 cut(s) 179
Hin1II CATG 3 cut(s) 93, 172, 325
HinfI GANTC 1 cut(s) 244
HpaII CCGG 4 cut(s) 133, 182, 365, 492
HphI GGTGA 2 cut(s) 94, 170
Hpy166II GTNNAC 1 cut(s) 385
Hpy188I TCNGA 1 cut(s) 340
Hpy188III TCNNGA 7 cut(s) 52, 133, 169, 196, 354, 365, 397
Hpy8I GTNNAC 1 cut(s) 385
Hpy99I CGWCG 3 cut(s) 128, 197, 289
HpyAV CCTTC 2 cut(s) 74, 351
HpyCH4III ACNGT 1 cut(s) 382
HpyCH4IV ACGT 2 cut(s) 192, 287
HpyCH4V TGCA 1 cut(s) 467
HpyF10VI GCNNNNNNNGC 1 cut(s) 235
HpyF3I CTNAG 1 cut(s) 174
HpySE526I ACGT 2 cut(s) 192, 287
Hsp92I GRCGYC 1 cut(s) 179
Hsp92II CATG 3 cut(s) 93, 172, 325
Kpn2I TCCGGA 2 cut(s) 132, 364
Kzo9I GATC 3 cut(s) 54, 198, 350
LguI GCTCTTC 1 cut(s) 41
LmnI GCTCC 3 cut(s) 51, 135, 367
LweI GCATC 1 cut(s) 16
MaeII ACGT 2 cut(s) 192, 287
MaeIII GTNAC 1 cut(s) 158
MalI GATC 3 cut(s) 56, 200, 352
MboI GATC 3 cut(s) 54, 198, 350
MboII GAAGA 3 cut(s) 28, 453, 511
MhlI GDGCHC 1 cut(s) 364
MnlI CCTC 6 cut(s) 50, 59, 116, 176, 240, 499
MroI TCCGGA 2 cut(s) 132, 364
MseI TTAA 1 cut(s) 15
MslI CAYNNNNRTG 1 cut(s) 320
MspI CCGG 4 cut(s) 133, 182, 365, 492
MvnI CGCG 1 cut(s) 53
MwoI GCNNNNNNNGC 1 cut(s) 235
NdeI CATATG 1 cut(s) 203
NdeII GATC 3 cut(s) 54, 198, 350
NlaIII CATG 3 cut(s) 93, 172, 325
NlaIV GGNNCC 2 cut(s) 327, 363
NmuCI GTSAC 1 cut(s) 158
NruI TCGCGA 1 cut(s) 53
NspI RCATGY 1 cut(s) 93
PagI TCATGA 1 cut(s) 168
PciSI GCTCTTC 1 cut(s) 41
PfeI GAWTC 1 cut(s) 244
Ple19I CGATCG 1 cut(s) 57
PspFI CCCAGC 1 cut(s) 263
PspN4I GGNNCC 2 cut(s) 327, 363
PsrI GAACNNNNNNTAC 2 cut(s) 377, 409
PvuI CGATCG 1 cut(s) 57
RgaI GCGATCGC 1 cut(s) 57
RruI TCGCGA 1 cut(s) 53
RsaI GTAC 2 cut(s) 143, 386
RsaNI GTAC 2 cut(s) 142, 385
RseI CAYNNNNRTG 1 cut(s) 320
SapI GCTCTTC 1 cut(s) 41
SaqAI TTAA 1 cut(s) 15
Sau3AI GATC 3 cut(s) 54, 198, 350
SduI GDGCHC 1 cut(s) 364
SetI ASST 7 cut(s) 21, 140, 168, 195, 216, 265, 290
SfaAI GCGATCGC 1 cut(s) 57
SfaNI GCATC 1 cut(s) 16
SgfI GCGATCGC 1 cut(s) 57
SmiMI CAYNNNNRTG 1 cut(s) 320
SmlI CTYRAG 2 cut(s) 292, 397
SmoI CTYRAG 2 cut(s) 292, 397
SsiI CCGC 1 cut(s) 486
TaaI ACNGT 1 cut(s) 382
TaiI ACGT 2 cut(s) 195, 290
TaqI TCGA 2 cut(s) 126, 449
TatI WGTACW 1 cut(s) 384
TfiI GAWTC 1 cut(s) 244
Tru1I TTAA 1 cut(s) 15
Tru9I TTAA 1 cut(s) 15
TscAI CASTG 1 cut(s) 322
TseFI GTSAC 1 cut(s) 158
Tsp45I GTSAC 1 cut(s) 158
TspRI CASTG 1 cut(s) 322
XagI CCTNNNNNAGG 1 cut(s) 493
XceI RCATGY 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.