FvH4_7g06210

Transmembrane protein 97-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001495
Physical Location & Seq
Reverse (-)
6332639 .. 6334038
1400 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g06210.t1

Sequence Viewer

Length: 552 bp
ATGGGTGCTATAATGAAGCTAGTCGATGCTGTACTGTGTTTGTTCTTCATCATAATGATAGTGTCGCCTATGGTGCACGCGCAGTTTGTTCTGCCTCTGAGCCTCTTCCCGGAGTGGATGGTGGAGCAGAAGAAGTGGTATATGCGCGAGTTCAATGACTATTTCTATGTCGAGAAGCCTGATTTCTTTATTGGTCTGGTATTGGTTGAGCTTCTGTTCCAGTGGCCTCTTGCCCTTGCCATCTTGTACGGCATGTTGAGTTCCAAGAATTGGTACAACACCACCTGCTTGGTTCATGGTGCCTCTTTCTTCACCACCATGGTGACAGCATTAGCAGGACTCATTGGATCAGGCAAGGCATCTGATAAGGTCTTAACCTTGTACTACACTTTCTTGGGGTTCGCTGTTCTGACCCTGCTTCGTGGGTTGATGCCACCACCAACTACTTCAAACAATGACAAAGCAAGAAGAAAAAGGATTGGCGCACCGAAGAAAATGATAGTGTACTCACAGATTTCTTTCTTTGTTTCAATAATTCTTTTACATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2781

Protein Analysis

184

Amino Acids

20.85

Weight (kDa)

9.37

Isoelectric Point (pI)

30.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EBP PF05241 40 - 119 1.9e-12 EXPERA (EXPanded EBP superfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 293
Acc36I ACCTGC 1 cut(s) 293
AccB1I GGYRCC 1 cut(s) 299
AccB7I CCANNNNNTGG 1 cut(s) 270
AccII CGCG 2 cut(s) 80, 147
AclWI GGATC 1 cut(s) 355
AfaI GTAC 5 cut(s) 33, 248, 275, 383, 506
AfiI CCNNNNNNNGG 2 cut(s) 109, 270
AflIII ACRYGT 1 cut(s) 544
AgsI TTSAA 3 cut(s) 154, 450, 531
AleI CACNNNNGTG 1 cut(s) 320
AluBI AGCT 2 cut(s) 19, 211
AluI AGCT 2 cut(s) 19, 211
Alw21I GWGCWC 1 cut(s) 78
Alw44I GTGCAC 1 cut(s) 74
AlwI GGATC 1 cut(s) 355
AoxI GGCC 1 cut(s) 224
ApaLI GTGCAC 1 cut(s) 74
AspLEI GCGC 3 cut(s) 82, 147, 485
AsuC2I CCSGG 1 cut(s) 110
AsuHPI GGTGA 2 cut(s) 304, 334
BaeGI GKGCMC 1 cut(s) 78
BanI GGYRCC 1 cut(s) 299
Bbv12I GWGCWC 1 cut(s) 78
BccI CCATC 2 cut(s) 112, 248
BceAI ACGGC 1 cut(s) 265
BcnI CCSGG 1 cut(s) 110
BfaI CTAG 1 cut(s) 20
BfuAI ACCTGC 1 cut(s) 293
Bme1390I CCNGG 1 cut(s) 110
BmiI GGNNCC 1 cut(s) 301
BmrFI CCNGG 1 cut(s) 110
BmsI GCATC 3 cut(s) 16, 368, 420
BpuMI CCSGG 1 cut(s) 110
BsaJI CCNNGG 1 cut(s) 318
Bsc4I CCNNNNNNNGG 2 cut(s) 109, 270
Bse1I ACTGG 1 cut(s) 220
BseDI CCNNGG 1 cut(s) 318
BseGI GGATG 1 cut(s) 123
BseLI CCNNNNNNNGG 2 cut(s) 109, 270
BseMII CTCAG 1 cut(s) 89
BseNI ACTGG 1 cut(s) 220
BseSI GKGCMC 1 cut(s) 78
Bsh1236I CGCG 2 cut(s) 80, 147
BshFI GGCC 1 cut(s) 226
BshNI GGYRCC 1 cut(s) 299
BsiHKAI GWGCWC 1 cut(s) 78
BsiSI CCGG 1 cut(s) 110
BslI CCNNNNNNNGG 2 cut(s) 109, 270
BsnI GGCC 1 cut(s) 226
Bsp1286I GDGCHC 1 cut(s) 78
Bsp143I GATC 1 cut(s) 347
Bsp19I CCATGG 1 cut(s) 318
BspANI GGCC 1 cut(s) 226
BspCNI CTCAG 1 cut(s) 90
BspFNI CGCG 2 cut(s) 80, 147
BspLI GGNNCC 1 cut(s) 301
BspMI ACCTGC 1 cut(s) 293
BspPI GGATC 1 cut(s) 355
BspT107I GGYRCC 1 cut(s) 299
BsrI ACTGG 1 cut(s) 220
BssECI CCNNGG 1 cut(s) 318
BssMI GATC 1 cut(s) 347
BssT1I CCWWGG 1 cut(s) 318
Bst4CI ACNGT 1 cut(s) 36
Bst6I CTCTTC 1 cut(s) 110
BstC8I GCNNGC 1 cut(s) 78
BstDEI CTNAG 1 cut(s) 98
BstDSI CCRYGG 1 cut(s) 318
BstF5I GGATG 1 cut(s) 123
BstFNI CGCG 2 cut(s) 80, 147
BstHHI GCGC 3 cut(s) 82, 147, 485
BstKTI GATC 1 cut(s) 350
BstMBI GATC 1 cut(s) 347
BstMWI GCNNNNNNNGC 1 cut(s) 73
BstNSI RCATGY 2 cut(s) 256, 548
BstSCI CCNGG 1 cut(s) 108
BstSLI GKGCMC 1 cut(s) 78
BstUI CGCG 2 cut(s) 80, 147
BstXI CCANNNNNNTGG 1 cut(s) 289
BsuRI GGCC 1 cut(s) 226
BtgI CCRYGG 1 cut(s) 318
BtsCI GGATG 1 cut(s) 123
BtsIMutI CAGTG 1 cut(s) 227
BveI ACCTGC 1 cut(s) 293
Cac8I GCNNGC 1 cut(s) 78
CfoI GCGC 3 cut(s) 82, 147, 485
Csp6I GTAC 5 cut(s) 32, 247, 274, 382, 505
CviAII CATG 4 cut(s) 253, 296, 319, 545
CviJI RGCY 5 cut(s) 19, 102, 178, 211, 226
CviKI_1 RGCY 5 cut(s) 19, 102, 178, 211, 226
CviQI GTAC 5 cut(s) 32, 247, 274, 382, 505
DdeI CTNAG 1 cut(s) 98
DpnI GATC 1 cut(s) 349
DpnII GATC 1 cut(s) 347
Eam1104I CTCTTC 1 cut(s) 110
EarI CTCTTC 1 cut(s) 110
Eco130I CCWWGG 1 cut(s) 318
EcoT14I CCWWGG 1 cut(s) 318
ErhI CCWWGG 1 cut(s) 318
FaeI CATG 4 cut(s) 256, 299, 322, 548
FatI CATG 4 cut(s) 252, 295, 318, 544
FokI GGATG 1 cut(s) 130
FspBI CTAG 1 cut(s) 20
GlaI GCGC 3 cut(s) 81, 146, 484
HaeIII GGCC 1 cut(s) 226
HapII CCGG 1 cut(s) 110
HhaI GCGC 3 cut(s) 82, 147, 485
Hin1II CATG 4 cut(s) 256, 299, 322, 548
Hin6I GCGC 3 cut(s) 80, 145, 483
HinP1I GCGC 3 cut(s) 80, 145, 483
HinfI GANTC 1 cut(s) 339
HpaII CCGG 1 cut(s) 110
HphI GGTGA 2 cut(s) 304, 334
Hpy166II GTNNAC 2 cut(s) 76, 505
Hpy188I TCNGA 3 cut(s) 99, 364, 411
Hpy188III TCNNGA 1 cut(s) 172
Hpy8I GTNNAC 2 cut(s) 76, 505
HpyCH4III ACNGT 1 cut(s) 36
HpyCH4V TGCA 1 cut(s) 76
HpyF10VI GCNNNNNNNGC 1 cut(s) 73
HpyF3I CTNAG 1 cut(s) 98
Hsp92II CATG 4 cut(s) 256, 299, 322, 548
HspAI GCGC 3 cut(s) 80, 145, 483
Kzo9I GATC 1 cut(s) 347
LmnI GCTCC 1 cut(s) 124
LpnPI CCDG 8 cut(s) 123, 182, 192, 233, 298, 321, 336, 428
LweI GCATC 3 cut(s) 16, 368, 420
MaeI CTAG 1 cut(s) 20
MaeIII GTNAC 1 cut(s) 322
MalI GATC 1 cut(s) 349
MboI GATC 1 cut(s) 347
MboII GAAGA 6 cut(s) 37, 97, 142, 301, 480, 502
MhlI GDGCHC 1 cut(s) 78
MluCI AATT 2 cut(s) 268, 534
MlyI GAGTC 1 cut(s) 333
MnlI CCTC 4 cut(s) 105, 113, 237, 313
MseI TTAA 1 cut(s) 374
MslI CAYNNNNRTG 3 cut(s) 53, 317, 320
MspI CCGG 1 cut(s) 110
MspR9I CCNGG 1 cut(s) 110
MvnI CGCG 2 cut(s) 80, 147
MwoI GCNNNNNNNGC 1 cut(s) 73
NciI CCSGG 1 cut(s) 110
NcoI CCATGG 1 cut(s) 318
NdeII GATC 1 cut(s) 347
NlaIII CATG 4 cut(s) 256, 299, 322, 548
NlaIV GGNNCC 1 cut(s) 301
NmuCI GTSAC 1 cut(s) 322
NspI RCATGY 2 cut(s) 256, 548
OliI CACNNNNGTG 1 cut(s) 320
PaqCI CACCTGC 1 cut(s) 293
PciI ACATGT 1 cut(s) 544
PflMI CCANNNNNTGG 1 cut(s) 270
PfoI TCCNGGA 1 cut(s) 108
PleI GAGTC 1 cut(s) 333
PpsI GAGTC 1 cut(s) 333
PscI ACATGT 1 cut(s) 544
PspN4I GGNNCC 1 cut(s) 301
RsaI GTAC 5 cut(s) 33, 248, 275, 383, 506
RsaNI GTAC 5 cut(s) 32, 247, 274, 382, 505
RseI CAYNNNNRTG 3 cut(s) 53, 317, 320
SaqAI TTAA 1 cut(s) 374
Sau3AI GATC 1 cut(s) 347
SchI GAGTC 1 cut(s) 333
ScrFI CCNGG 1 cut(s) 110
SduI GDGCHC 1 cut(s) 78
SetI ASST 5 cut(s) 21, 213, 287, 372, 380
SfaNI GCATC 3 cut(s) 16, 368, 420
SmiMI CAYNNNNRTG 3 cut(s) 53, 317, 320
Sse9I AATT 2 cut(s) 268, 534
SspMI CTAG 1 cut(s) 20
StyD4I CCNGG 1 cut(s) 108
StyI CCWWGG 1 cut(s) 318
TaaI ACNGT 1 cut(s) 36
TaqI TCGA 2 cut(s) 24, 171
TasI AATT 2 cut(s) 268, 534
TatI WGTACW 3 cut(s) 31, 381, 504
Tru1I TTAA 1 cut(s) 374
Tru9I TTAA 1 cut(s) 374
TscAI CASTG 1 cut(s) 227
TseFI GTSAC 1 cut(s) 322
Tsp45I GTSAC 1 cut(s) 322
TspDTI ATGAA 3 cut(s) 29, 37, 284
TspRI CASTG 1 cut(s) 227
Van91I CCANNNNNTGG 1 cut(s) 270
VneI GTGCAC 1 cut(s) 74
XceI RCATGY 2 cut(s) 256, 548
XspI CTAG 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.