FvH4_7g05110

Phosphoinositide 3-kinase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001323
Physical Location & Seq
Forward (+)
5480201 .. 5488174
7974 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g05110.t6

Sequence Viewer

Length: 4656 bp
ATGGGGAACAAGATAGCGCGGACGACGCAGGTGTCGGCGTCGGAGTATTACCTGCACGACCTGCCGTCTTCGTATAACCTAGTGCTGAAAGAGGTTCTAGGTCGCGGACGCTTCTTCAAGTCGATTCAGTGCAAGCACGACGAGGGTTTGGTCCTCGTCAAGGTCTATTTCAAGCGCGGCGACTCCATCGATCTCCGCGACTACGAGCGCCGTCTCTTCCACATCAAGGAGACCTTCCGCGCCCTCGATCACCCTCACGTCTGGCCCTTCCAGTTCTGGCAAGAGACCGATAAAGCGGCTTATCTTGTGCGGCAGTACTTGTTCAACAACCTCCATGATCGATTGAGCACCCGCCCCTTTCTCAGTCTCATCGAGAAGAAATGGCTGGCTTTCCAGTTGCTTCTGGCACTGAAACAGTGCCATGACAAAGGAATATGTCACGGTGATATCAAGTGTGAGAATGTGCTGGTCACTTCTTGGAACTGGCTCTATCTTGCCGACTTTGCATCCTTCAAACCCACCTACATCCCCTACGACGACCCTTCTGATTTCTCTTTTTTCTATGACACTGGTGGAAGAAGGCTTTGTTATCTTGCTCCCGAGAGATTTTATGAGCATGGAGGTGAGATGCAAGTTGCACAAGATGCACCATTAAGACCATCCATGGATATATTTGCTGTAGGGTGTGTGATTGCTGAGCTTTTCCTCGAGGGTCAGCCCCTATTTGAACTCTCTCAACTTCTCGCTTATCGTAGAGGGCAATACGATCCTAGTCAACTTCTTGAAAAGATACCAGATTTTGGAATCCGCAAAATGATACTTCATATGATTCAGTTGGAACCGGAGTTACGACTTGCTGCTGATAGCTACCTGCAGGAGTATACAACTATTGTGTTTCCGAGTTACTTCTCTCCGTTTCTGCATAACTTTCATTGTTTCTGGAATCCGCTTCATTGTGATATGAGGATTGCACTTTGCCAGAGTGTTTTTCCTGAGATACTGAAACAAATGATGAGCAATAGGTCAACTCAGGACACTAGCACCGGACTTGGAACTCCTTCAAACATCCATGCTGTTAACAGTAAATCTTCTCAGGACACGAAGAACATGAATATGCCAAAGGGCTCACTGGGAAAGAAAGTAGAGATGGATAAAGGGTTAAAACGTGATCAATATGAACTTCTTGGTGATATCAATACCCTTCTCAGGGATGTTAAACAAAGTAATCATTATTCTAATACAAAGACAATGCCGGAGGACAACACTGGTTCTGCATTCTCCCAAAATCTTGGAAACTATGGCATGCAATCTCCTGGTGAATTACTTCAGACAATCTCTCGTGCATTTAGGAGAAATGATCATCACTTTCTAAAAAAGATTACAATGAATGATTTGAATTCATTGATGTCTAAGTATGACAGCCAATCAGATACCTTTGGCATGCCCTTTTTACCATTACCTGAAGATAGTTTGAGATGTGAGGGCATGGTTCTGATTACCTCTCTGCTATGTTCTTGCATACGCAATGTCAAATTGCCTCATTTAAGGAGGAGGGCGATACTTTTGCTGAAGTCATCTGCATTGTATATTGATGATGATAACCGGCTGCAGCGTGTAATTCCTTATGTTGTTGCAATGCTTTCAGATCAAGCTGCAATTGTGCGTTGTGCAGCCTTGGAGACGTTATGTGACATTCTCCCTTTAGTTCGAGATTTTCCTCCTAGTGATGCAAAAATTTTTCCTGAGTATATTCTGCCAATGCTTTCCATGCTTCCTGATGATTCAGAGGAAAGTGTAAGGATATGTTATGCCAGTAATATAGCTAAGCTGGCACTAACTGCTTATGGTTTCTTAGTTCACTCAATAACCTTGAGTGAGGCTGGTGTGCTTGATGAAGTAAGTTCTAAGAACCAACTGGCATCATCTAGTGAGGCATCTGGTCAGCTCCACAAGTTAAACGGTGATGCACAGCTTGCCCAGTTGAGAAAGTCCATAGCTGAAGTCATTCAGGAGCTTGTTATGGGTCCTAGGCAAACTCCCAATATCAGGAGAGCACTCCTTCAGGATATTAGCAATCTATGCTGCTTCTTTGGCCAGAGGCAGAGTAATGACTTTTTGTTACCGATTCTCCCTGCTTTTCTGAATGATCGAGATGAGCAGCTAAGAGCAGTATTCTATGGCCAAATTGTGTATGTGTGTTTCTTTGTAGGTCAAAGAAGTGTGGAAGAATATCTTTTACCTTATATTGAGCAGGCTGTAAGTGATTCTACAGAGGCCGTTATTGTCAATGCATTGGACTGTTTGGCCATTTTGTGCAGAAGCGGTTACTTGCGGAAGAGGATACTCCTGGAAATGATTGAGCGGGCATTTCCATTACTATGTTATCCTAGTCAGTGGGTAAGAAGGTCAGCTGTCAGTTTTATTGCAGCCAGCAGTGAGTGCTTGGGTGCAGTAGATTCATATGTATTCCTTGCTCCCGTTATTCGCCCCCTTCTTCGTAGACAGCCAGCATCTCTAGCTTCTGAGAAGGCTCTCTTTTCATGTTTGAAGCCTCCTGTCTCGAGACAGGTATTTTACCAAGTTCTAGAAAATGCAAGGAGTTCTGACATGTTGGAAAGACAGAGAAAAATATGGTACAATTCTCGGCCTCAATCTAAACAATGGGAAAATGTGGATTTACTTCATAAAGGGATTGCAGAATTGAATTCGATGAGGAGCTGGACAGATGACCAAGAAAATCCCGAGGGTCAAAAACGTGCTGGAAATGAATTGCAACAGGGGAAGCTAACTGAATGTGATGATGGTGTGGCTAAGTTTGGATGTATGGGAAGCTTTACGCACAAGGCTTCGAGCACAGTTGACATTCACGATCCCTTATCCTCTGAAAAGTTGCAGTACTCGGGCTTTATGTGGCCACAGGGAAGTACTGTGAATAGCTTCATGTGTGATAAATCATCAGTAGGGATACCTCTGTACTCCTTTAGCATGGACAGGCAAGCAGTGGGAGTTACTTCTGCTTCATCTGATTCTCCATTACAAGTGAGTTCTGTAGGAGTTGGTGCGTCATCTATGCCATGGATGGATCCAGTAAATAAATCTTTTAGTTTAGCTAGTACTGTTCCAGCACCCAAACTGGTGTCAGGATCATTCAACATCGGCAGTGGTTCTAAACAATTTTATCGAGTAGTCCATGAACCAGATGGTAGGGATAATGACCAAACAGCCTTTGTCAATAGCAAGTTTCAAGATATGGGGTTAACTAGTGCTACAAAAGCAAGTTCTATTACTGTGGAAGATGCATCCTCTACAAGTGATCTAACGGGACTGCCCTCTTCTGCAAGAGCTTCATCAATTCCAGATTCTGGTTGGAGGCCTCGTGGAGTTTTGGTTGCGCACTTGCAAGAGCATCGGTCTGCTGTCAATGACATAGCTATTTCAACTGATCATAGCTTCTTTGTGAGTGCATCTGATGATTCCACTGTCAAGGTCTGGGATTCGAGAAAGTTGGAGAAGGACATCTCATTTAGGTCAAGATTGACTTATCATCTGGAGGGAAGCCGTGCACTATGCTCTGCAATGCTTCGGGGCTGTGCTCAAGTTGTAGTTGGGGCATGTGATGGCATGATACATATGTTTTCTGTTGATTACATTTCCAGAGGTCTTGGTAATGTAGTTGAGAAGTACTCAGGGGTTGCTGATATCAAGAAGAAAGACACAAAAGAAGGGGCAATACTTAGCCTTTTGAATTTCTCAGCTGACAACTGTGCAAATCAAATGGTTATGTACAGCACCCAAAATTGTGGGATCCATCTTTGGGATATAAGAACGAATTCAGATTCATGGACACTGAAAGCAACTCCGGAAGAGGGCTATGTGTCTTCCCTAGTAACAGGGCCTTGTGAAAATTGGTTTGTATCAGGATCTTCAAGGGGTGTGCTCACTCTTTGGGATATGAGGTTCCTTGTTCCTGTGAACTCATGGCAGTATTCTGCTGTTTGCCCCATAGAGAAGATGTGTCTTTTCCTTCCTCCTCCAAATGCTTCTGTATCTGCTGCTGCAAGGCCCCTTGTTTATGTAGCTGCAGGCTGCAACGAAGTTTCTCTTTGGAATGCAGAAAATGGCACCTGCCATCAGGTGTTGAGGGTTGCCAGTTACGAAAGTGACACTGAAATGTCTGAGGTGCCTTGGGCCTTGTCCAGATCATCAGCTAAAAATTCTAAAGCGGATATGAGACGGAATGTTAATCCTCATTACAGAGTTGATGAACTGAATGAACCACCTCCTCGGATTCCTGGCATCCGTTCATTGCTTCCCTTACCTGGGGGTGATTTGTTAACCGGGGGAACTGACTTGAAGATACGTCGATGGGATCATTACAGCCCCGAACGAAGTTACTGTATTTGTGGGCCAAACTTGAAGGGTGTGGGAAATGATGATTTCTATGGAATACGATCTAGCTTTGGTGTGCAAGTTGTGCAGGAGACGAAAAGACGGCCTCTAACAACAAAGTTGACAGCCAAGGCAGTTCTTGCAGCTGCTGCCACTGATACTGCTGGCAGCCATCGTGATTCAATCCTTTCATTGGCCTCTGTTAAGTTGAACCACAGACACTTAATATCAAGCAGTAGAGACGGGGCAATCAAGGTCTGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000011 GO:0000151 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005737 GO:0005768 GO:0005770 GO:0005942 GO:0006355 GO:0006357 GO:0006464 GO:0006468 GO:0006605 GO:0006623 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006886 GO:0006914 GO:0006996 GO:0007033 GO:0007034 GO:0007154 GO:0007165 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009555 GO:0009846 GO:0009856 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010008 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0016236 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019219 GO:0019222 GO:0019538 GO:0019898 GO:0022414 GO:0023052 GO:0030242 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031410 GO:0031461 GO:0031967 GO:0031975 GO:0031982 GO:0032182 GO:0032501 GO:0032502 GO:0032507 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0033036 GO:0033365 GO:0034067 GO:0034243 GO:0034271 GO:0034272 GO:0034613 GO:0035032 GO:0035556 GO:0036211 GO:0042886 GO:0043130 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0044706 GO:0045053 GO:0045184 GO:0045185 GO:0045324 GO:0045893 GO:0045935 GO:0045944 GO:0046907 GO:0048015 GO:0048017 GO:0048229 GO:0048308 GO:0048518 GO:0048522 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051235 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051651 GO:0051704 GO:0051716 GO:0060255 GO:0061695 GO:0061919 GO:0065007 GO:0065008 GO:0070727 GO:0071561 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0080008 GO:0080090 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0120095 GO:0140096 GO:1901564 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04136 ko04138 ko04140 ko04371 map04136 map04138 map04140 map04371
Pfam Domains
Protein Families
HEAT Pkinase WD40

Protein Analysis

1552

Amino Acids

173.76

Weight (kDa)

7.18

Isoelectric Point (pI)

50.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 28 - 239 1.1e-07 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 29 - 292 3.9e-21 Protein kinase domain
VPS15-like_hel PF22956 499 - 861 9.3e-124 VPS15-like, helical domain
Beta-prop_WDR5 PF25175 1127 - 1214 1.6e-08 WDR5 beta-propeller domain
WD40_Prp19 PF24814 1127 - 1332 3.5e-10 Prp19 WD40 domain
WD40 PF00400 1128 - 1165 9.6e-09 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 1131 - 1325 6.7e-10 THOC3 beta-propeller domain
WD40_Gbeta PF25391 1133 - 1319 6.6e-07 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 1138 - 1339 1e-06 WDHD1 first WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 19, 4133
AasI GACNNNNNNGTC 1 cut(s) 31
AbsI CCTCGAGG 1 cut(s) 707
Acc16I TGCGCA 1 cut(s) 3393
Acc36I ACCTGC 5 cut(s) 19, 60, 69, 879, 4133
AccB1I GGYRCC 2 cut(s) 4121, 4180
AccB7I CCANNNNNTGG 2 cut(s) 800, 3362
AccBSI CCGCTC 1 cut(s) 2362
AccI GTMKAC 2 cut(s) 881, 2500
AccII CGCG 5 cut(s) 19, 105, 177, 198, 240
AccIII TCCGGA 1 cut(s) 3859
AclWI GGATC 9 cut(s) 761, 2864, 3077, 3090, 3151, 3799, 3812, 3928, 4377
AcoI YGGCCR 4 cut(s) 2092, 2179, 2304, 2912
AcsI RAATTY 6 cut(s) 1396, 1734, 2704, 3745, 3829, 4213
AcuI CTGAAG 5 cut(s) 1310, 1482, 1589, 2019, 2045
AcyI GRCGYC 1 cut(s) 38
AfaI GTAC 8 cut(s) 317, 2636, 2897, 2926, 2975, 3115, 3683, 3785
AflIII ACRYGT 1 cut(s) 2607
AhdI GACNNNNNGTC 1 cut(s) 64
AhlI ACTAGT 1 cut(s) 3260
AjiI CACGTC 1 cut(s) 259
AjnI CCWGG 4 cut(s) 1312, 2346, 4291, 4318
AloI GAACNNNNNNTCC 2 cut(s) 3941, 3973
Alw21I GWGCWC 6 cut(s) 350, 2056, 2855, 3565, 3595, 3939
Alw44I GTGCAC 1 cut(s) 3561
AlwI GGATC 9 cut(s) 761, 2864, 3077, 3090, 3151, 3799, 3812, 3928, 4377
AlwNI CAGNNNCTG 2 cut(s) 3362, 4538
Ama87I CYCGRG 5 cut(s) 599, 707, 2560, 2741, 2899
Aor13HI TCCGGA 1 cut(s) 3859
ApaLI GTGCAC 1 cut(s) 3561
ApoI RAATTY 6 cut(s) 1396, 1734, 2704, 3745, 3829, 4213
Asp700I GAANNNNTTC 5 cut(s) 1057, 1323, 2004, 2936, 3829
AspA2I CCTAGG 1 cut(s) 2027
AspLEI GCGC 5 cut(s) 19, 177, 210, 242, 3394
AspS9I GGNCC 7 cut(s) 151, 264, 2024, 3893, 4062, 4188, 4406
AsuC2I CCSGG 1 cut(s) 4339
AsuHPI GGTGA 7 cut(s) 242, 455, 635, 1199, 1328, 1973, 4337
AvaI CYCGRG 5 cut(s) 599, 707, 2560, 2741, 2899
AvaII GGWCC 2 cut(s) 151, 2024
AvrII CCTAGG 1 cut(s) 2027
BaeGI GKGCMC 1 cut(s) 3565
BaeI ACNNNNGTAYC 4 cut(s) 2957, 2990, 3617, 3650
BalI TGGCCA 4 cut(s) 2094, 2181, 2306, 2914
BamHI GGATCC 2 cut(s) 3082, 3804
BanI GGYRCC 2 cut(s) 4121, 4180
BanII GRGCYC 1 cut(s) 1127
BarI GAAGNNNNNNTAC 2 cut(s) 3714, 3746
BauI CACGAG 2 cut(s) 1338, 3375
BbsI GAAGAC 2 cut(s) 60, 3870
Bbv12I GWGCWC 6 cut(s) 350, 2056, 2855, 3565, 3595, 3939
BceAI ACGGC 5 cut(s) 49, 195, 2261, 3543, 4508
BcgI CGANNNNNNTGC 4 cut(s) 1546, 1580, 3389, 3423
BciT130I CCWGG 4 cut(s) 1314, 2348, 4293, 4320
BciVI GTATCC 2 cut(s) 2334, 2958
BclI TGATCA 3 cut(s) 1168, 1357, 3442
BcnI CCSGG 1 cut(s) 4339
BcuI ACTAGT 1 cut(s) 3260
BfmI CTRYAG 6 cut(s) 678, 872, 1607, 2268, 3048, 4080
BfoI RGCGCY 1 cut(s) 211
BfuAI ACCTGC 5 cut(s) 19, 60, 69, 879, 4133
BfuI GTATCC 2 cut(s) 2334, 2958
BlnI CCTAGG 1 cut(s) 2027
BlpI GCTNAGC 2 cut(s) 696, 1824
BmcAI AGTACT 5 cut(s) 317, 2897, 2926, 3115, 3683
Bme1390I CCNGG 5 cut(s) 1314, 2348, 4293, 4320, 4339
Bme18I GGWCC 2 cut(s) 151, 2024
BmeRI GACNNNNNGTC 1 cut(s) 64
BmeT110I CYCGRG 5 cut(s) 599, 707, 2560, 2741, 2899
BmgBI CACGTC 1 cut(s) 259
BmgT120I GGNCC 7 cut(s) 151, 264, 2024, 3893, 4062, 4188, 4406
BmiI GGNNCC 8 cut(s) 840, 2025, 3084, 3806, 3959, 4064, 4123, 4182
BmrFI CCNGG 5 cut(s) 1314, 2348, 4293, 4320, 4339
BmrI ACTGGG 2 cut(s) 1139, 1972
BmuI ACTGGG 2 cut(s) 1139, 1972
BpiI GAAGAC 2 cut(s) 60, 3870
BplI GAGNNNNNCTC 2 cut(s) 3668, 3700
BpmI CTGGAG 1 cut(s) 3569
Bpu1102I GCTNAGC 2 cut(s) 696, 1824
BpuEI CTTGAG 2 cut(s) 1891, 3579
BpuMI CCSGG 1 cut(s) 4339
Bsa29I ATCGAT 2 cut(s) 189, 340
BsaHI GRCGYC 1 cut(s) 38
BsaI GGTCTC 2 cut(s) 224, 278
BsaWI WCCGGW 3 cut(s) 841, 1043, 3859
BsaXI ACNNNNNCTCC 6 cut(s) 2112, 2142, 3361, 3372, 3391, 3402
Bse118I RCCGGY 1 cut(s) 1602
Bse3DI GCAATG 4 cut(s) 1531, 1641, 3582, 4304
BseAI TCCGGA 1 cut(s) 3859
BseBI CCWGG 4 cut(s) 1314, 2348, 4293, 4320
BseCI ATCGAT 2 cut(s) 189, 340
BseGI GGATG 9 cut(s) 506, 525, 659, 1065, 1216, 2825, 3084, 3299, 4296
BseMI GCAATG 4 cut(s) 1531, 1641, 3582, 4304
BseRI GAGGAG 4 cut(s) 1564, 2728, 4020, 4272
BseSI GKGCMC 1 cut(s) 3565
BsgI GTGCAG 5 cut(s) 38, 1689, 2335, 2469, 4496
Bsh1236I CGCG 5 cut(s) 19, 105, 177, 198, 240
BshNI GGYRCC 2 cut(s) 4121, 4180
BshVI ATCGAT 2 cut(s) 189, 340
BsiHKAI GWGCWC 6 cut(s) 350, 2056, 2855, 3565, 3595, 3939
BsiHKCI CYCGRG 5 cut(s) 599, 707, 2560, 2741, 2899
BsiSI CCGG 6 cut(s) 842, 1044, 1253, 1603, 3860, 4338
BslFI GGGAC 1 cut(s) 3336
BsmBI CGTCTC 5 cut(s) 218, 1673, 4225, 4475, 4623
BsmFI GGGAC 1 cut(s) 3336
BsmI GAATGC 2 cut(s) 1274, 4114
Bso31I GGTCTC 2 cut(s) 224, 278
BsoBI CYCGRG 5 cut(s) 599, 707, 2560, 2741, 2899
Bsp1286I GDGCHC 7 cut(s) 350, 1127, 2056, 2855, 3565, 3595, 3939
Bsp13I TCCGGA 1 cut(s) 3859
Bsp1407I TGTACA 1 cut(s) 3783
Bsp1720I GCTNAGC 2 cut(s) 696, 1824
Bsp19I CCATGG 2 cut(s) 663, 3074
BspDI ATCGAT 2 cut(s) 189, 340
BspEI TCCGGA 1 cut(s) 3859
BspFNI CGCG 5 cut(s) 19, 105, 177, 198, 240
BspLI GGNNCC 8 cut(s) 840, 2025, 3084, 3806, 3959, 4064, 4123, 4182
BspMAI CTGCAG 3 cut(s) 876, 1611, 4084
BspMI ACCTGC 5 cut(s) 19, 60, 69, 879, 4133
BspPI GGATC 9 cut(s) 761, 2864, 3077, 3090, 3151, 3799, 3812, 3928, 4377
BspT107I GGYRCC 2 cut(s) 4121, 4180
BspTNI GGTCTC 2 cut(s) 224, 278
BsrBI CCGCTC 1 cut(s) 2362
BsrDI GCAATG 4 cut(s) 1531, 1641, 3582, 4304
BsrFI RCCGGY 1 cut(s) 1602
BsrGI TGTACA 1 cut(s) 3783
BssAI RCCGGY 1 cut(s) 1602
BssNAI GTATAC 1 cut(s) 882
BssNI GRCGYC 1 cut(s) 38
BssSI CACGAG 2 cut(s) 1338, 3375
BssT1I CCWWGG 6 cut(s) 663, 1674, 2027, 3074, 4184, 4518
Bst1107I GTATAC 1 cut(s) 882
Bst2BI CACGAG 2 cut(s) 1338, 3375
Bst2UI CCWGG 4 cut(s) 1314, 2348, 4293, 4320
Bst6I CTCTTC 4 cut(s) 221, 2330, 3337, 3858
BstACI GRCGYC 1 cut(s) 38
BstAPI GCANNNNNTGC 9 cut(s) 61, 644, 1838, 1973, 2079, 2439, 4474, 4529, 4538
BstAUI TGTACA 1 cut(s) 3783
BstDSI CCRYGG 2 cut(s) 663, 3074
BstENI CCTNNNNNAGG 1 cut(s) 158
BstF5I GGATG 9 cut(s) 506, 525, 659, 1065, 1216, 2825, 3084, 3299, 4296
BstFNI CGCG 5 cut(s) 19, 105, 177, 198, 240
BstH2I RGCGCY 1 cut(s) 211
BstHHI GCGC 5 cut(s) 19, 177, 210, 242, 3394
BstNI CCWGG 4 cut(s) 1314, 2348, 4293, 4320
BstNSI RCATGY 4 cut(s) 1306, 1444, 2611, 3615
BstSCI CCNGG 5 cut(s) 1312, 2346, 4291, 4318, 4337
BstSFI CTRYAG 6 cut(s) 678, 872, 1607, 2268, 3048, 4080
BstSLI GKGCMC 1 cut(s) 3565
BstUI CGCG 5 cut(s) 19, 105, 177, 198, 240
BstV2I GAAGAC 2 cut(s) 60, 3870
BstX2I RGATCY 3 cut(s) 3082, 3804, 3920
BstXI CCANNNNNNTGG 2 cut(s) 1289, 3800
BstYI RGATCY 3 cut(s) 3082, 3804, 3920
BstZ17I GTATAC 1 cut(s) 882
Bsu15I ATCGAT 2 cut(s) 189, 340
BsuI GTATCC 2 cut(s) 2334, 2958
BsuTUI ATCGAT 2 cut(s) 189, 340
BtgI CCRYGG 2 cut(s) 663, 3074
BtrI CACGTC 1 cut(s) 259
BtsCI GGATG 9 cut(s) 506, 525, 659, 1065, 1216, 2825, 3084, 3299, 4296
BtsI GCAGTG 3 cut(s) 2440, 3006, 3166
BveI ACCTGC 5 cut(s) 19, 60, 69, 879, 4133
CaiI CAGNNNCTG 2 cut(s) 3362, 4538
CfoI GCGC 5 cut(s) 19, 177, 210, 242, 3394
Cfr10I RCCGGY 1 cut(s) 1602
Cfr13I GGNCC 7 cut(s) 151, 264, 2024, 3893, 4062, 4188, 4406
ClaI ATCGAT 2 cut(s) 189, 340
CseI GACGC 4 cut(s) 27, 34, 117, 3051
Csp6I GTAC 8 cut(s) 316, 2635, 2896, 2925, 2974, 3114, 3682, 3784
CviQI GTAC 8 cut(s) 316, 2635, 2896, 2925, 2974, 3114, 3682, 3784
DrdI GACNNNNNNGTC 1 cut(s) 31
DriI GACNNNNNGTC 1 cut(s) 64
DseDI GACNNNNNNGTC 1 cut(s) 31
EaeI YGGCCR 4 cut(s) 2092, 2179, 2304, 2912
Eam1104I CTCTTC 4 cut(s) 221, 2330, 3337, 3858
Eam1105I GACNNNNNGTC 1 cut(s) 64
EarI CTCTTC 4 cut(s) 221, 2330, 3337, 3858
Eco130I CCWWGG 6 cut(s) 663, 1674, 2027, 3074, 4184, 4518
Eco147I AGGCCT 1 cut(s) 3373
Eco24I GRGCYC 1 cut(s) 1127
Eco31I GGTCTC 2 cut(s) 224, 278
Eco32I GATATC 3 cut(s) 448, 1192, 3700
Eco47I GGWCC 2 cut(s) 151, 2024
Eco57I CTGAAG 5 cut(s) 1310, 1482, 1589, 2019, 2045
Eco88I CYCGRG 5 cut(s) 599, 707, 2560, 2741, 2899
EcoNI CCTNNNNNAGG 1 cut(s) 158
EcoO109I RGGNCCY 3 cut(s) 2024, 3893, 4062
EcoRI GAATTC 3 cut(s) 1396, 2704, 3829
EcoRII CCWGG 4 cut(s) 1312, 2346, 4291, 4318
EcoRV GATATC 3 cut(s) 448, 1192, 3700
EcoT14I CCWWGG 6 cut(s) 663, 1674, 2027, 3074, 4184, 4518
EcoT22I ATGCAT 2 cut(s) 2293, 3301
EcoT38I GRGCYC 1 cut(s) 1127
ErhI CCWWGG 6 cut(s) 663, 1674, 2027, 3074, 4184, 4518
Esp3I CGTCTC 5 cut(s) 218, 1673, 4225, 4475, 4623
FaqI GGGAC 1 cut(s) 3336
FauI CCCGC 2 cut(s) 359, 2355
FauNDI CATATG 3 cut(s) 825, 2461, 3630
FbaI TGATCA 3 cut(s) 1168, 1357, 3442
FblI GTMKAC 2 cut(s) 881, 2500
FokI GGATG 9 cut(s) 493, 512, 646, 1052, 1223, 2832, 3091, 3286, 4283
FriOI GRGCYC 1 cut(s) 1127
FspI TGCGCA 1 cut(s) 3393
GlaI GCGC 5 cut(s) 18, 176, 209, 241, 3393
GsuI CTGGAG 1 cut(s) 3569
HaeII RGCGCY 1 cut(s) 211
HapII CCGG 6 cut(s) 842, 1044, 1253, 1603, 3860, 4338
HgaI GACGC 4 cut(s) 27, 34, 117, 3051
HhaI GCGC 5 cut(s) 19, 177, 210, 242, 3394
Hin1I GRCGYC 1 cut(s) 38
Hin6I GCGC 5 cut(s) 17, 175, 208, 240, 3392
HinP1I GCGC 5 cut(s) 17, 175, 208, 240, 3392
HincII GTYRAC 7 cut(s) 776, 1026, 1078, 2860, 3258, 4335, 4512
HindII GTYRAC 7 cut(s) 776, 1026, 1078, 2860, 3258, 4335, 4512
HindIII AAGCTT 1 cut(s) 2830
HpaI GTTAAC 3 cut(s) 1078, 3258, 4335
HpaII CCGG 6 cut(s) 842, 1044, 1253, 1603, 3860, 4338
HphI GGTGA 7 cut(s) 242, 455, 635, 1199, 1328, 1973, 4337
Hpy99I CGWCG 5 cut(s) 28, 43, 143, 539, 4365
HpyCH4IV ACGT 5 cut(s) 258, 1165, 1682, 2755, 4360
HpySE526I ACGT 5 cut(s) 258, 1165, 1682, 2755, 4360
Hsp92I GRCGYC 1 cut(s) 38
HspAI GCGC 5 cut(s) 17, 175, 208, 240, 3392
Kpn2I TCCGGA 1 cut(s) 3859
Ksp22I TGATCA 3 cut(s) 1168, 1357, 3442
KspAI GTTAAC 3 cut(s) 1078, 3258, 4335
LmnI GCTCC 5 cut(s) 601, 1950, 2011, 2479, 2715
MaeII ACGT 5 cut(s) 258, 1165, 1682, 2755, 4360
MbiI CCGCTC 1 cut(s) 2362
MfeI CAATTG 1 cut(s) 1656
MflI RGATCY 3 cut(s) 3082, 3804, 3920
MhlI GDGCHC 7 cut(s) 350, 1127, 2056, 2855, 3565, 3595, 3939
MlsI TGGCCA 4 cut(s) 2094, 2181, 2306, 2914
MluNI TGGCCA 4 cut(s) 2094, 2181, 2306, 2914
MlyI GAGTC 1 cut(s) 176
MmeI TCCRAC 5 cut(s) 21, 816, 2592, 3347, 3486
Mox20I TGGCCA 4 cut(s) 2094, 2181, 2306, 2914
Mph1103I ATGCAT 2 cut(s) 2293, 3301
MroI TCCGGA 1 cut(s) 3859
MroXI GAANNNNTTC 5 cut(s) 1057, 1323, 2004, 2936, 3829
MscI TGGCCA 4 cut(s) 2094, 2181, 2306, 2914
MslI CAYNNNNRTG 4 cut(s) 621, 1112, 2377, 4169
Msp20I TGGCCA 4 cut(s) 2094, 2181, 2306, 2914
MspA1I CMGCKG 3 cut(s) 2411, 3755, 4535
MspI CCGG 6 cut(s) 842, 1044, 1253, 1603, 3860, 4338
MspR9I CCNGG 5 cut(s) 1314, 2348, 4293, 4320, 4339
MunI CAATTG 1 cut(s) 1656
Mva1269I GAATGC 2 cut(s) 1274, 4114
MvaI CCWGG 4 cut(s) 1314, 2348, 4293, 4320
MvnI CGCG 5 cut(s) 19, 105, 177, 198, 240
NciI CCSGG 1 cut(s) 4339
NcoI CCATGG 2 cut(s) 663, 3074
NdeI CATATG 3 cut(s) 825, 2461, 3630
NlaIV GGNNCC 8 cut(s) 840, 2025, 3084, 3806, 3959, 4064, 4123, 4182
NmeAIII GCCGAG 1 cut(s) 2623
NmuCI GTSAC 4 cut(s) 437, 469, 1688, 4160
NsbI TGCGCA 1 cut(s) 3393
NsiI ATGCAT 2 cut(s) 2293, 3301
NspI RCATGY 4 cut(s) 1306, 1444, 2611, 3615
PaeI GCATGC 2 cut(s) 1306, 1444
PaeR7I CTCGAG 2 cut(s) 707, 2560
PaqCI CACCTGC 2 cut(s) 19, 4133
PceI AGGCCT 1 cut(s) 3373
PciI ACATGT 1 cut(s) 2607
PcsI WCGNNNNNNNCGW 1 cut(s) 195
PctI GAATGC 2 cut(s) 1274, 4114
PdmI GAANNNNTTC 5 cut(s) 1057, 1323, 2004, 2936, 3829
PflMI CCANNNNNTGG 2 cut(s) 800, 3362
PfoI TCCNGGA 1 cut(s) 2346
PleI GAGTC 1 cut(s) 176
PpsI GAGTC 1 cut(s) 176
PpuMI RGGWCCY 1 cut(s) 2024
PscI ACATGT 1 cut(s) 2607
Psp5II RGGWCCY 1 cut(s) 2024
Psp6I CCWGG 4 cut(s) 1312, 2346, 4291, 4318
PspGI CCWGG 4 cut(s) 1312, 2346, 4291, 4318
PspN4I GGNNCC 8 cut(s) 840, 2025, 3084, 3806, 3959, 4064, 4123, 4182
PspPI GGNCC 7 cut(s) 151, 264, 2024, 3893, 4062, 4188, 4406
PspPPI RGGWCCY 1 cut(s) 2024
PspXI VCTCGAGB 1 cut(s) 707
PsrI GAACNNNNNNTAC 2 cut(s) 831, 863
PstI CTGCAG 3 cut(s) 876, 1611, 4084
PstNI CAGNNNCTG 2 cut(s) 3362, 4538
PsuI RGATCY 3 cut(s) 3082, 3804, 3920
PvuII CAGCTG 3 cut(s) 2411, 3755, 4535
RsaI GTAC 8 cut(s) 317, 2636, 2897, 2926, 2975, 3115, 3683, 3785
RsaNI GTAC 8 cut(s) 316, 2635, 2896, 2925, 2974, 3114, 3682, 3784
RseI CAYNNNNRTG 4 cut(s) 621, 1112, 2377, 4169
Sau96I GGNCC 7 cut(s) 151, 264, 2024, 3893, 4062, 4188, 4406
SbfI CCTGCAGG 1 cut(s) 876
ScaI AGTACT 5 cut(s) 317, 2897, 2926, 3115, 3683
SchI GAGTC 1 cut(s) 176
ScrFI CCNGG 5 cut(s) 1314, 2348, 4293, 4320, 4339
SdaI CCTGCAGG 1 cut(s) 876
SduI GDGCHC 7 cut(s) 350, 1127, 2056, 2855, 3565, 3595, 3939
SfcI CTRYAG 6 cut(s) 678, 872, 1607, 2268, 3048, 4080
Sfr274I CTCGAG 2 cut(s) 707, 2560
SinI GGWCC 2 cut(s) 151, 2024
SlaI CTCGAG 2 cut(s) 707, 2560
SmiMI CAYNNNNRTG 4 cut(s) 621, 1112, 2377, 4169
SmlI CTYRAG 4 cut(s) 707, 1870, 2560, 3594
SmoI CTYRAG 4 cut(s) 707, 1870, 2560, 3594
SpeI ACTAGT 1 cut(s) 3260
SphI GCATGC 2 cut(s) 1306, 1444
Sse8387I CCTGCAGG 1 cut(s) 876
SseBI AGGCCT 1 cut(s) 3373
StuI AGGCCT 1 cut(s) 3373
StyD4I CCNGG 5 cut(s) 1312, 2346, 4291, 4318, 4337
StyI CCWWGG 6 cut(s) 663, 1674, 2027, 3074, 4184, 4518
TaiI ACGT 5 cut(s) 261, 1168, 1685, 2758, 4363
TaqII GACCGA 2 cut(s) 302, 3399
TatI WGTACW 7 cut(s) 315, 2895, 2924, 2973, 3113, 3681, 3783
TauI GCSGC 3 cut(s) 180, 299, 313
TseFI GTSAC 4 cut(s) 437, 469, 1688, 4160
Tsp45I GTSAC 4 cut(s) 437, 469, 1688, 4160
TspGWI ACGGA 3 cut(s) 903, 4249, 4289
Van91I CCANNNNNTGG 2 cut(s) 800, 3362
VneI GTGCAC 1 cut(s) 3561
VpaK11BI GGWCC 2 cut(s) 151, 2024
XagI CCTNNNNNAGG 1 cut(s) 158
XapI RAATTY 6 cut(s) 1396, 1734, 2704, 3745, 3829, 4213
XbaI TCTAGA 1 cut(s) 2584
XceI RCATGY 4 cut(s) 1306, 1444, 2611, 3615
XcmI CCANNNNNNNNNTGG 2 cut(s) 3197, 3363
XhoI CTCGAG 2 cut(s) 707, 2560
XmaJI CCTAGG 1 cut(s) 2027
XmiI GTMKAC 2 cut(s) 881, 2500
XmnI GAANNNNTTC 5 cut(s) 1057, 1323, 2004, 2936, 3829
ZrmI AGTACT 5 cut(s) 317, 2897, 2926, 3115, 3683
Zsp2I ATGCAT 2 cut(s) 2293, 3301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.