Rw1G010170

Phosphoinositide 3-kinase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001323
Physical Location & Seq
Reverse (-)
22506111 .. 22517030
10920 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G010170.1

Sequence Viewer

Length: 3597 bp
ATGTTAAGCAAAATTGCAAAATTAGGTTTTAAGGTTAAAAGTGACATTTTATCTTTAGATTGGACTAGAAAACTTACCCGCTTGCAGCTACGGAAGATGCTGGAGTTGGCCACGGGCCACGCTTGTCGCTGTTTAACAAAAGACAGATGTAAAATCCTGGAATGCTGGAACAAAAACTTAAGCAGTTTTAGAAGCTACAAGACTTTAGCATATTCTTGTATACAACACTTCGAGCCTTCTTTACAATTTTGCTTATGTATTTGGTCCTCTTATTTAGAAAGATTTTGTGAGCATGGAGGTGAGATGCAAGTTGCACAAGATGCACCATTAAGACCATCTATGGATATCTTTGCTGTAGGGAGTGTGATTGCTGAGCTTTTTCTTGAGAGTCGGCCACTATTTGAACTCTCTCAACTTCTCACTTATCGTAGAGGGCAATACGGTCCTAGTCAACTTCTTGAAAAGATACCAGATTGTGGAATCCGCAAAATGATACTTCATATGATTCAGTTAGAACCGGAGCTGCGACTTTCTGCTGATAGCTACCTTCAGGAGTATACAACGATTGTGTTTCCGAGTTACTTCTTTCTCTCCGTTGCACTTTGCCAGAGTGTTTTTCCTGAGATACTGAAACAAATGATGAGCAATAGGTCAACTCAGGACACTAGCACTGGTCATGGAACTCCTTCAAACATCCATGCTGTTAGAGGTAAATCTTCTCAGGACATGATTGCAATGCAGAACATGAATTTGACAAAGGGCTCACTGGGAAAGAAAGTAGAGATGGATAAAGGTTTCAAACGTGATCAATTTGAATTTCTCGGTGATATCAATACCCTTCTTAGGGATGTTAAACAAAGTAATCATTATTCTAATACGAAGCCAGTGGTGAAGGACAACACCAAGTCTGCATTTTCCCAAAATCTTGGAAACTATGGCATGCAATCTCCTGGTGAATTACGTCAGACTATCTCCAATGCGTTTAGGAGAAATGATCATCCTTTTCTAAAAAAGATTACAATGAACGATTTGAATTCATTGATATCCAAGTATGACAGCCAGTCAGATACCTTTGGTATGCCTTTTTTACCATTACCTGAAGACAGTTTGAGATGTGAGGGCATTGTTCTGATTACCTCTTTGCTATGTTCCTGCCTACGCAATGTCAAATTGCCTCATTTAAGGAGGAGGGCGATACTTTTGCTGAAGTCATCTGCATTGTATATTGATGATGAAAACCGGTTGCAGCGTGTAATTCCATATGTTGTTGCAATGCTTTCAGATCAAGCTGCAATTGTGCATTGTGCAGCCTTGGAGATATTATGTGACATTCTCCCTTTAGTTCGAGATTTTCCTCCTAGTGATGCAAAAAAAATTCCGGATGTGAAGATATGTTATGCCAGTACTATAGCTAAGCTGGCACTAACTGCTTATGATTTCTTAGTTCACTCAATAACCTTGAGTGAGGCTGGTGTGCTTGATGAATTAAGTTCTCCAAAGAAGCTACTATCATCATCTAGTGAGACATCTAGTCAGCTCCACAAGGTAAATAATGATGCACAGCTTGCCCAGTTGAGGAAGTCCATAGCTGCAGTCATTCAAGAGCTTGTTATGGGTCCCAAGCAAACTCCCAATATCAGGAGAGCACTCCTTGAGGATATCAGCAATCTATGCTGCTTCTTTGGCCAGAGGCAGAGTAATTTTCAGCTAAGACCAGTATTCTACAGGCAAATTGTGCATGTATGCTTCTTTGTAGGTCAAAGAAGTGTGGAAGAATATCTTTTACCTTATATTGAGCAGGCTGTAAGTGATGCTACAGAGGCTGCTATTGTCAATGCATTGGACTGTTTGGCCATTTTGTGCAAAAGTAGTTACTTGCGCAAGAGGATACTCCTGGAAATGATTGAGCGTGCATTTCCATTGTCATGCTATCCTAGTCATTGTAGATTCCTATGTATTCCTTGCTCCTGTTATTCGTCCCCTTCTTGTAGACAGCCAGCATCTCTAGCTTCTGAGAAGGCTCTCCTTTCATGTTTGAAGCCTCCTGTCTCAAGACAAGTATTTTACCTAGTTCTAGAAAATGCAAGGAGTACAGACATGTTGGAAAGACAGAGAAAGATATGCTTTATGTGGCCACAGGGACGTACTGTGAATAGCTTCATGTGTGATAAATCATCAGTAGGGATACCTTCGTACTCATTTAGCATGGACAGGCGAGCAGTGAGAGTTCCTCCTGCTCCATCTGATTCTTCATTACAAGTGAGTTCTGTAGGAGTTGGTGCATCATCCACACTTTCCCATTTAAATAAATCTTTTAGTTTAGCTGGTTCTGTTCCAGCACCCAAGCTCGTGTCAGGATCATTCAACATCAGCAGTGGTTCTAAACAATTCTACAGAGTAGTACATGAACCGGATGGTACGGATTATGACCAAACAACCTTTGTCAATAGCAAGTTTCAAGATATGGGGTTATCTAGTGCTACAAAAGCAAGTTCTATTACTGTGGAAGATACATCCTCTACAAGGGATCTAACAGGATTGCCCTCTTCTACAAGAGCTTCATCAATTCCAGATTCTGGTTGGAGGCCTCGTTGGGTTTTCATCAAGGTATGGGATTCGAGAAAGTTGGAAAAGGACATCTCATTTAGGTCAAGATTGACTTATCATCTGAAGGGAAGCCGTGCACTATGTTCTGCAATGCTCCAGGGCTGCGCTCAAGTTGTAGTTGGAGCATGTGATGGCATGATACATATGTTTTCTCCTTCTGAATTTCTCTGCTGCAACTATGCAAATCAGATGGTTATGTACAGCACCCAAAATTGTGGGATCCATCTTTGGGATATGAGAACGAATTCAGATTCTTGGACATTGAAAGCAACTCCGGAGGAGGGCTATGTGTCTTCCCTAGTAACAGGCCCTTGTGAAAATTGGTTTGTGTCAGGATCTTCAAGGGGTGTGGTCACTCTGTGGGATATGAGGTTCCTTATTCCTGTGAATTCATGGCAGTATTCTGCTGTTTGCCCCATAGAGAAAATGTGTCTTTTCCTTCCTCCACCAAATGCTTCTGTATCTGCTGCTGTTGTTTATGTTGCTGCAGGTTGCAATGAAGTTTCTCTTTGGAATGCAGAAAATGGCACCTGCCACCAGGTGTTGAGGGTTGCTAGTTATGAAAGTGATACTGAAATGTCTGAGATGCCTTGGGCCTTGGCAAGATCATCAACTAAAAATTCCAAAGTGGATATGAGACGGAATGTCAATCCTCATTACAGAGTTGATGAACTGAATGAACCACCTCCTCGCATTCCTGGCATCCGTTCATTGCTTCCCTTAACTGGGGGTGATTTGTTAACTGGGGAAACCGACTTGAAGATACATCGATGGGATCATTACAGCCCTGATCGAAGTTACTGTATTTGTGGGCCAAACTTGAAGGGTAGACGGCCTCTAACAACAAAGTTGACAGCCAAGGCAGTTCTTGCAGCTGCTGCCACTGATACTACTGGCTGCCATCGTGTTTCAATCCTTTCATTGGCCTCTGTTAAGTTGAATCAAGGACTCTTAATATCAAGCAGTAGAGATGGGGCAATCAAGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000011 GO:0000151 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005737 GO:0005768 GO:0005770 GO:0005942 GO:0006355 GO:0006357 GO:0006464 GO:0006468 GO:0006605 GO:0006623 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006886 GO:0006914 GO:0006996 GO:0007033 GO:0007034 GO:0007154 GO:0007165 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009555 GO:0009846 GO:0009856 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010008 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0016236 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019219 GO:0019222 GO:0019538 GO:0019898 GO:0022414 GO:0023052 GO:0030242 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031410 GO:0031461 GO:0031967 GO:0031975 GO:0031982 GO:0032182 GO:0032501 GO:0032502 GO:0032507 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0033036 GO:0033365 GO:0034067 GO:0034243 GO:0034271 GO:0034272 GO:0034613 GO:0035032 GO:0035556 GO:0036211 GO:0042886 GO:0043130 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0044706 GO:0045053 GO:0045184 GO:0045185 GO:0045324 GO:0045893 GO:0045935 GO:0045944 GO:0046907 GO:0048015 GO:0048017 GO:0048229 GO:0048308 GO:0048518 GO:0048522 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051235 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051651 GO:0051704 GO:0051716 GO:0060255 GO:0061695 GO:0061919 GO:0065007 GO:0065008 GO:0070727 GO:0071561 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0080008 GO:0080090 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0120095 GO:0140096 GO:1901564 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04136 ko04138 ko04140 ko04371 map04136 map04138 map04140 map04371
Pfam Domains
Protein Families
HEAT Pkinase WD40

Protein Analysis

1198

Amino Acids

133.82

Weight (kDa)

8.86

Isoelectric Point (pI)

53.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VPS15-like_hel PF22956 378 - 647 7.5e-59 VPS15-like, helical domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3146
Acc16I TGCGCA 1 cut(s) 1880
Acc36I ACCTGC 2 cut(s) 3086, 3146
AccB1I GGYRCC 1 cut(s) 3134
AccB7I CCANNNNNTGG 2 cut(s) 476, 2576
AccI GTMKAC 4 cut(s) 220, 557, 1990, 3436
AccIII TCCGGA 2 cut(s) 1378, 2881
AciI CCGC 2 cut(s) 79, 484
AclWI GGATC 6 cut(s) 2365, 2535, 2821, 2834, 2950, 3390
AcoI YGGCCR 5 cut(s) 108, 392, 1684, 1851, 2132
AcsI RAATTY 8 cut(s) 748, 815, 1033, 1374, 2768, 2851, 2995, 3226
AcuI CTGAAG 4 cut(s) 533, 1119, 1226, 2690
AdeI CACNNNGTG 2 cut(s) 2967, 3148
AfaI GTAC 7 cut(s) 1405, 2092, 2146, 2195, 2403, 2419, 2807
AflII CTTAAG 1 cut(s) 178
AflIII ACRYGT 1 cut(s) 2097
AgeI ACCGGT 1 cut(s) 1239
AhdI GACNNNNNGTC 3 cut(s) 1060, 1530, 3251
AjnI CCWGG 6 cut(s) 156, 949, 1893, 2703, 3144, 3304
AloI GAACNNNNNNTCC 2 cut(s) 2963, 2995
Alw21I GWGCWC 2 cut(s) 1648, 2686
Alw26I GTCTC 3 cut(s) 1517, 2053, 3238
Alw44I GTGCAC 1 cut(s) 2682
AlwI GGATC 6 cut(s) 2365, 2535, 2821, 2834, 2950, 3390
AlwNI CAGNNNCTG 3 cut(s) 1823, 2576, 3485
Aor13HI TCCGGA 2 cut(s) 1378, 2881
ApaLI GTGCAC 1 cut(s) 2682
ApoI RAATTY 8 cut(s) 748, 815, 1033, 1374, 2768, 2851, 2995, 3226
AsiGI ACCGGT 1 cut(s) 1239
Asp700I GAANNNNTTC 3 cut(s) 685, 2156, 2851
AspLEI GCGC 2 cut(s) 1881, 2714
AspS9I GGNCC 7 cut(s) 115, 264, 443, 1616, 2915, 3201, 3419
AsuHPI GGTGA 5 cut(s) 311, 836, 901, 965, 3350
AvaII GGWCC 3 cut(s) 264, 443, 1616
BaeGI GKGCMC 1 cut(s) 2686
BaeI ACNNNNGTAYC 4 cut(s) 2177, 2210, 2738, 2771
BalI TGGCCA 4 cut(s) 110, 1686, 1853, 2134
BamHI GGATCC 1 cut(s) 2826
BanI GGYRCC 1 cut(s) 3134
BanII GRGCYC 1 cut(s) 764
BauI CACGAG 1 cut(s) 2348
BbsI GAAGAC 2 cut(s) 1107, 2892
Bbv12I GWGCWC 2 cut(s) 1648, 2686
BceAI ACGGC 2 cut(s) 2664, 3455
BcgI CGANNNNNNTGC 2 cut(s) 1183, 1217
BciT130I CCWGG 6 cut(s) 158, 951, 1895, 2705, 3146, 3306
BciVI GTATCC 2 cut(s) 1881, 2178
BclI TGATCA 2 cut(s) 805, 994
BcoDI GTCTC 3 cut(s) 1517, 2053, 3238
BfmI CTRYAG 8 cut(s) 354, 1407, 1590, 1723, 1815, 2268, 2392, 3093
BfrI CTTAAG 1 cut(s) 178
BfuAI ACCTGC 2 cut(s) 3086, 3146
BfuI GTATCC 2 cut(s) 1881, 2178
BlpI GCTNAGC 2 cut(s) 372, 1413
BmcAI AGTACT 1 cut(s) 1405
Bme1390I CCNGG 6 cut(s) 158, 951, 1895, 2705, 3146, 3306
Bme18I GGWCC 3 cut(s) 264, 443, 1616
BmeRI GACNNNNNGTC 3 cut(s) 1060, 1530, 3251
BmgT120I GGNCC 7 cut(s) 115, 264, 443, 1616, 2915, 3201, 3419
BmiI GGNNCC 5 cut(s) 1617, 1618, 2828, 2981, 3136
BmrFI CCNGG 6 cut(s) 158, 951, 1895, 2705, 3146, 3306
BmrI ACTGGG 4 cut(s) 776, 1564, 3342, 3360
BmuI ACTGGG 4 cut(s) 776, 1564, 3342, 3360
BpiI GAAGAC 2 cut(s) 1107, 2892
BplI GAGNNNNNCTC 2 cut(s) 2215, 2247
BpmI CTGGAG 2 cut(s) 122, 2687
Bpu1102I GCTNAGC 2 cut(s) 372, 1413
BpuEI CTTGAG 5 cut(s) 404, 1480, 1673, 2035, 2700
Bsa29I ATCGAT 1 cut(s) 3376
BsaJI CCNNGG 6 cut(s) 111, 1311, 2704, 3197, 3204, 3465
BsaWI WCCGGW 5 cut(s) 517, 1239, 1378, 2410, 2881
BsaXI ACNNNNNCTCC 2 cut(s) 2575, 2605
Bse118I RCCGGY 1 cut(s) 1239
Bse3DI GCAATG 6 cut(s) 741, 1168, 1278, 2703, 3109, 3317
BseAI TCCGGA 2 cut(s) 1378, 2881
BseBI CCWGG 6 cut(s) 158, 951, 1895, 2705, 3146, 3306
BseCI ATCGAT 1 cut(s) 3376
BseDI CCNNGG 6 cut(s) 111, 1311, 2704, 3197, 3204, 3465
BseGI GGATG 8 cut(s) 693, 853, 997, 1387, 2285, 2419, 2513, 3309
BseMI GCAATG 6 cut(s) 741, 1168, 1278, 2703, 3109, 3317
BseMII CTCAG 6 cut(s) 363, 612, 671, 734, 2004, 3180
BseRI GAGGAG 3 cut(s) 1201, 2900, 3285
BseSI GKGCMC 1 cut(s) 2686
BsgI GTGCAG 1 cut(s) 1326
BshNI GGYRCC 1 cut(s) 3134
BshTI ACCGGT 1 cut(s) 1239
BshVI ATCGAT 1 cut(s) 3376
BsiHKAI GWGCWC 2 cut(s) 1648, 2686
BsiSI CCGG 5 cut(s) 518, 1240, 1379, 2411, 2882
BslFI GGGAC 3 cut(s) 1602, 1963, 2154
BsmAI GTCTC 3 cut(s) 1517, 2053, 3238
BsmBI CGTCTC 1 cut(s) 3238
BsmFI GGGAC 3 cut(s) 1602, 1963, 2154
BsmI GAATGC 3 cut(s) 167, 3127, 3300
Bsp1286I GDGCHC 3 cut(s) 764, 1648, 2686
Bsp13I TCCGGA 2 cut(s) 1378, 2881
Bsp1407I TGTACA 1 cut(s) 2805
Bsp1720I GCTNAGC 2 cut(s) 372, 1413
BspACI CCGC 2 cut(s) 79, 484
BspCNI CTCAG 6 cut(s) 364, 613, 670, 733, 2005, 3181
BspDI ATCGAT 1 cut(s) 3376
BspEI TCCGGA 2 cut(s) 1378, 2881
BspLI GGNNCC 5 cut(s) 1617, 1618, 2828, 2981, 3136
BspMAI CTGCAG 2 cut(s) 1594, 3097
BspMI ACCTGC 2 cut(s) 3086, 3146
BspPI GGATC 6 cut(s) 2365, 2535, 2821, 2834, 2950, 3390
BspT107I GGYRCC 1 cut(s) 3134
BspTI CTTAAG 1 cut(s) 178
BsrDI GCAATG 6 cut(s) 741, 1168, 1278, 2703, 3109, 3317
BsrFI RCCGGY 1 cut(s) 1239
BsrGI TGTACA 1 cut(s) 2805
BssAI RCCGGY 1 cut(s) 1239
BssECI CCNNGG 6 cut(s) 111, 1311, 2704, 3197, 3204, 3465
BssNAI GTATAC 2 cut(s) 221, 558
BssSI CACGAG 1 cut(s) 2348
BssT1I CCWWGG 4 cut(s) 1311, 3197, 3204, 3465
Bst1107I GTATAC 2 cut(s) 221, 558
Bst2BI CACGAG 1 cut(s) 2348
Bst2UI CCWGG 6 cut(s) 158, 951, 1895, 2705, 3146, 3306
Bst4CI ACNGT 6 cut(s) 443, 1106, 1847, 2149, 2503, 3410
Bst6I CTCTTC 1 cut(s) 2551
BstAFI CTTAAG 1 cut(s) 178
BstAPI GCANNNNNTGC 7 cut(s) 320, 1427, 1565, 1671, 1735, 3476, 3485
BstAUI TGTACA 1 cut(s) 2805
BstC8I GCNNGC 8 cut(s) 83, 941, 1419, 1566, 1800, 1911, 1998, 2217
BstDSI CCRYGG 1 cut(s) 111
BstENI CCTNNNNNAGG 1 cut(s) 2521
BstF5I GGATG 8 cut(s) 693, 853, 997, 1387, 2285, 2419, 2513, 3309
BstHHI GCGC 2 cut(s) 1881, 2714
BstMAI GTCTC 3 cut(s) 1517, 2053, 3238
BstNI CCWGG 6 cut(s) 158, 951, 1895, 2705, 3146, 3306
BstNSI RCATGY 4 cut(s) 943, 1742, 2101, 2736
BstSCI CCNGG 6 cut(s) 156, 949, 1893, 2703, 3144, 3304
BstSFI CTRYAG 8 cut(s) 354, 1407, 1590, 1723, 1815, 2268, 2392, 3093
BstSLI GKGCMC 1 cut(s) 2686
BstV2I GAAGAC 2 cut(s) 1107, 2892
BstX2I RGATCY 3 cut(s) 2527, 2826, 2942
BstXI CCANNNNNNTGG 2 cut(s) 926, 2822
BstYI RGATCY 3 cut(s) 2527, 2826, 2942
BstZ17I GTATAC 2 cut(s) 221, 558
Bsu15I ATCGAT 1 cut(s) 3376
BsuI GTATCC 2 cut(s) 1881, 2178
BsuTUI ATCGAT 1 cut(s) 3376
BtgI CCRYGG 1 cut(s) 111
BtsCI GGATG 8 cut(s) 693, 853, 997, 1387, 2285, 2419, 2513, 3309
BtsI GCAGTG 2 cut(s) 2226, 2380
BtsIMutI CAGTG 6 cut(s) 669, 764, 891, 2226, 2380, 3489
BveI ACCTGC 2 cut(s) 3086, 3146
Cac8I GCNNGC 8 cut(s) 83, 941, 1419, 1566, 1800, 1911, 1998, 2217
CaiI CAGNNNCTG 3 cut(s) 1823, 2576, 3485
CfoI GCGC 2 cut(s) 1881, 2714
Cfr10I RCCGGY 1 cut(s) 1239
Cfr13I GGNCC 7 cut(s) 115, 264, 443, 1616, 2915, 3201, 3419
ClaI ATCGAT 1 cut(s) 3376
CsiI ACCWGGT 1 cut(s) 3144
Csp6I GTAC 7 cut(s) 1404, 2091, 2145, 2194, 2402, 2418, 2806
CspAI ACCGGT 1 cut(s) 1239
CspCI CAANNNNNGTGG 2 cut(s) 2937, 2972
CviQI GTAC 7 cut(s) 1404, 2091, 2145, 2194, 2402, 2418, 2806
DraI TTTAAA 1 cut(s) 2304
DraIII CACNNNGTG 2 cut(s) 2967, 3148
DriI GACNNNNNGTC 3 cut(s) 1060, 1530, 3251
EaeI YGGCCR 5 cut(s) 108, 392, 1684, 1851, 2132
Eam1104I CTCTTC 1 cut(s) 2551
Eam1105I GACNNNNNGTC 3 cut(s) 1060, 1530, 3251
EarI CTCTTC 1 cut(s) 2551
Eco130I CCWWGG 4 cut(s) 1311, 3197, 3204, 3465
Eco147I AGGCCT 1 cut(s) 2587
Eco24I GRGCYC 1 cut(s) 764
Eco32I GATATC 4 cut(s) 346, 829, 1044, 1660
Eco47I GGWCC 3 cut(s) 264, 443, 1616
Eco57I CTGAAG 4 cut(s) 533, 1119, 1226, 2690
EcoNI CCTNNNNNAGG 1 cut(s) 2521
EcoO109I RGGNCCY 2 cut(s) 1616, 2915
EcoRI GAATTC 3 cut(s) 1033, 2851, 2995
EcoRII CCWGG 6 cut(s) 156, 949, 1893, 2703, 3144, 3304
EcoRV GATATC 4 cut(s) 346, 829, 1044, 1660
EcoT14I CCWWGG 4 cut(s) 1311, 3197, 3204, 3465
EcoT22I ATGCAT 1 cut(s) 1840
EcoT38I GRGCYC 1 cut(s) 764
ErhI CCWWGG 4 cut(s) 1311, 3197, 3204, 3465
Esp3I CGTCTC 1 cut(s) 3238
FalI AAGNNNNNCTT 8 cut(s) 1764, 1796, 2108, 2140, 2644, 2676, 3099, 3131
FaqI GGGAC 3 cut(s) 1602, 1963, 2154
FauI CCCGC 1 cut(s) 86
FauNDI CATATG 3 cut(s) 501, 1261, 2751
FbaI TGATCA 2 cut(s) 805, 994
FblI GTMKAC 4 cut(s) 220, 557, 1990, 3436
FokI GGATG 8 cut(s) 680, 860, 984, 1394, 2272, 2426, 2500, 3296
FriOI GRGCYC 1 cut(s) 764
FspI TGCGCA 1 cut(s) 1880
GlaI GCGC 2 cut(s) 1880, 2713
GsuI CTGGAG 2 cut(s) 122, 2687
HapII CCGG 5 cut(s) 518, 1240, 1379, 2411, 2882
HhaI GCGC 2 cut(s) 1881, 2714
Hin6I GCGC 2 cut(s) 1879, 2712
HinP1I GCGC 2 cut(s) 1879, 2712
HincII GTYRAC 4 cut(s) 452, 654, 3348, 3459
HindII GTYRAC 4 cut(s) 452, 654, 3348, 3459
HpaI GTTAAC 1 cut(s) 3348
HpaII CCGG 5 cut(s) 518, 1240, 1379, 2411, 2882
HphI GGTGA 5 cut(s) 311, 836, 901, 965, 3350
HpyCH4III ACNGT 6 cut(s) 443, 1106, 1847, 2149, 2503, 3410
HpyCH4IV ACGT 3 cut(s) 802, 961, 2143
HpySE526I ACGT 3 cut(s) 802, 961, 2143
HspAI GCGC 2 cut(s) 1879, 2712
KflI GGGWCCC 1 cut(s) 1616
Kpn2I TCCGGA 2 cut(s) 1378, 2881
Ksp22I TGATCA 2 cut(s) 805, 994
KspAI GTTAAC 1 cut(s) 3348
LmnI GCTCC 6 cut(s) 520, 1542, 1970, 2242, 2706, 2729
MabI ACCWGGT 1 cut(s) 3144
MaeII ACGT 3 cut(s) 802, 961, 2143
MaeIII GTNAC 7 cut(s) 41, 578, 1325, 1871, 2908, 2959, 3404
MfeI CAATTG 1 cut(s) 1293
MflI RGATCY 3 cut(s) 2527, 2826, 2942
MhlI GDGCHC 3 cut(s) 764, 1648, 2686
MlsI TGGCCA 4 cut(s) 110, 1686, 1853, 2134
MluNI TGGCCA 4 cut(s) 110, 1686, 1853, 2134
MlyI GAGTC 2 cut(s) 397, 3549
MmeI TCCRAC 4 cut(s) 2082, 2561, 2607, 2707
Mox20I TGGCCA 4 cut(s) 110, 1686, 1853, 2134
Mph1103I ATGCAT 1 cut(s) 1840
MroI TCCGGA 2 cut(s) 1378, 2881
MroXI GAANNNNTTC 3 cut(s) 685, 2156, 2851
MscI TGGCCA 4 cut(s) 110, 1686, 1853, 2134
MslI CAYNNNNRTG 2 cut(s) 297, 1924
Msp20I TGGCCA 4 cut(s) 110, 1686, 1853, 2134
MspA1I CMGCKG 1 cut(s) 3482
MspCI CTTAAG 1 cut(s) 178
MspI CCGG 5 cut(s) 518, 1240, 1379, 2411, 2882
MspR9I CCNGG 6 cut(s) 158, 951, 1895, 2705, 3146, 3306
MunI CAATTG 1 cut(s) 1293
Mva1269I GAATGC 3 cut(s) 167, 3127, 3300
MvaI CCWGG 6 cut(s) 158, 951, 1895, 2705, 3146, 3306
NdeI CATATG 3 cut(s) 501, 1261, 2751
NlaIV GGNNCC 5 cut(s) 1617, 1618, 2828, 2981, 3136
NmuCI GTSAC 3 cut(s) 41, 1325, 2959
NsbI TGCGCA 1 cut(s) 1880
NsiI ATGCAT 1 cut(s) 1840
NspI RCATGY 4 cut(s) 943, 1742, 2101, 2736
PaeI GCATGC 1 cut(s) 943
PaqCI CACCTGC 1 cut(s) 3146
PceI AGGCCT 1 cut(s) 2587
PciI ACATGT 1 cut(s) 2097
PctI GAATGC 3 cut(s) 167, 3127, 3300
PdmI GAANNNNTTC 3 cut(s) 685, 2156, 2851
PfeI GAWTC 8 cut(s) 480, 505, 1947, 2246, 2573, 2615, 2858, 3547
PflMI CCANNNNNTGG 2 cut(s) 476, 2576
PfoI TCCNGGA 2 cut(s) 156, 1893
PinAI ACCGGT 1 cut(s) 1239
PleI GAGTC 2 cut(s) 396, 3549
PpsI GAGTC 2 cut(s) 396, 3549
PpuMI RGGWCCY 1 cut(s) 1616
PscI ACATGT 1 cut(s) 2097
Psp5II RGGWCCY 1 cut(s) 1616
Psp6I CCWGG 6 cut(s) 156, 949, 1893, 2703, 3144, 3304
PspGI CCWGG 6 cut(s) 156, 949, 1893, 2703, 3144, 3304
PspN4I GGNNCC 5 cut(s) 1617, 1618, 2828, 2981, 3136
PspPI GGNCC 7 cut(s) 115, 264, 443, 1616, 2915, 3201, 3419
PspPPI RGGWCCY 1 cut(s) 1616
PstI CTGCAG 2 cut(s) 1594, 3097
PstNI CAGNNNCTG 3 cut(s) 1823, 2576, 3485
PsuI RGATCY 3 cut(s) 2527, 2826, 2942
PvuII CAGCTG 1 cut(s) 3482
RsaI GTAC 7 cut(s) 1405, 2092, 2146, 2195, 2403, 2419, 2807
RsaNI GTAC 7 cut(s) 1404, 2091, 2145, 2194, 2402, 2418, 2806
RseI CAYNNNNRTG 2 cut(s) 297, 1924
Sau96I GGNCC 7 cut(s) 115, 264, 443, 1616, 2915, 3201, 3419
ScaI AGTACT 1 cut(s) 1405
SchI GAGTC 2 cut(s) 397, 3549
ScrFI CCNGG 6 cut(s) 158, 951, 1895, 2705, 3146, 3306
SduI GDGCHC 3 cut(s) 764, 1648, 2686
SexAI ACCWGGT 1 cut(s) 3144
SfcI CTRYAG 8 cut(s) 354, 1407, 1590, 1723, 1815, 2268, 2392, 3093
SinI GGWCC 3 cut(s) 264, 443, 1616
SmiI ATTTAAAT 1 cut(s) 2304
SmiMI CAYNNNNRTG 2 cut(s) 297, 1924
SmlI CTYRAG 6 cut(s) 178, 383, 1459, 1652, 2050, 2715
SmoI CTYRAG 6 cut(s) 178, 383, 1459, 1652, 2050, 2715
SphI GCATGC 1 cut(s) 943
SseBI AGGCCT 1 cut(s) 2587
SsiI CCGC 2 cut(s) 79, 484
StuI AGGCCT 1 cut(s) 2587
StyD4I CCNGG 6 cut(s) 156, 949, 1893, 2703, 3144, 3304
StyI CCWWGG 4 cut(s) 1311, 3197, 3204, 3465
SwaI ATTTAAAT 1 cut(s) 2304
TaaI ACNGT 6 cut(s) 443, 1106, 1847, 2149, 2503, 3410
TaiI ACGT 3 cut(s) 805, 964, 2146
TaqI TCGA 5 cut(s) 231, 1345, 2618, 3376, 3400
TatI WGTACW 4 cut(s) 1403, 2090, 2401, 2805
TfiI GAWTC 8 cut(s) 480, 505, 1947, 2246, 2573, 2615, 2858, 3547
TscAI CASTG 6 cut(s) 676, 771, 891, 2226, 2380, 3496
TseFI GTSAC 3 cut(s) 41, 1325, 2959
Tsp45I GTSAC 3 cut(s) 41, 1325, 2959
TspGWI ACGGA 5 cut(s) 106, 583, 2435, 3262, 3302
TspRI CASTG 6 cut(s) 676, 771, 891, 2226, 2380, 3496
Van91I CCANNNNNTGG 2 cut(s) 476, 2576
Vha464I CTTAAG 1 cut(s) 178
VneI GTGCAC 1 cut(s) 2682
VpaK11BI GGWCC 3 cut(s) 264, 443, 1616
XagI CCTNNNNNAGG 1 cut(s) 2521
XapI RAATTY 8 cut(s) 748, 815, 1033, 1374, 2768, 2851, 2995, 3226
XbaI TCTAGA 1 cut(s) 2074
XceI RCATGY 4 cut(s) 943, 1742, 2101, 2736
XcmI CCANNNNNNNNNTGG 1 cut(s) 2577
XmiI GTMKAC 4 cut(s) 220, 557, 1990, 3436
XmnI GAANNNNTTC 3 cut(s) 685, 2156, 2851
ZrmI AGTACT 1 cut(s) 1405
Zsp2I ATGCAT 1 cut(s) 1840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.