pycom02g22410

Phosphoinositide 3-kinase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001323
Physical Location & Seq
Reverse (-)
20517247 .. 20520613
3367 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g22410.7

Sequence Viewer

Length: 1827 bp
ATGGGGAACAAGATCGCTCGAACGACGCAAGTGTCGGCGTCGGAGTACTACCTCCACGACCTACCATCGTCCTACAACCTGGTCCTGAAGGAGGTTCTAGGGCGGAGCCGCTTCTTCAAGTCGATTGAGTGCAAGCACGACGAGGGTTTGGTCCTCGTCAAGGTCTATTTCAAGCGCGGTGACTCCATTGATCTCCGCGAGTACGAGCGCCGCCTCTTCCTCATCAAGGAAACCTTCCGTGCTCTCGATCACCCTCACGTTTGGCCCTTCCAGTTTTGGCAAGAGACAGATAAAGCAGCTTATCTTGTAAGGCAATTTTTCTTCAACAATCTGCACGACCGGTTAAGCACCCGCCCGTTTCTCAGTCTAATCGAGAAGAAATGGTTGGCCTTTCAGTTACTTCTTGCTCTGAAGCAGTGCCATGATAAAGGAATATGTCACGGTGATATTAAGTGTGAGAATGTGTTGGTGACTTCTTGGAATTGGCTCTACCTTGCTGACTTTGCATCTTTCAAACCCACCTACATACCATATGACGATCCTTCTGATTTCTCTTTTTTCTTTGACACCGGAGGAAGAAGGCTTTGTTATCTTGCTCCTGAGAGATTTTATGAGCATGGAGGTGAGATGCAGGTTGCACAAGATGCACCATTAAGGCCATCTATGGACATATTTGCTGTAGGATGTGTAATTGCTGAGCTTTTCCTTGAGGGACAACCACTGTTCGAACTCTCTCAACTTCTTGCTTATCGTAGAGGGCAATACGATCCTAGCCAACTTCTTGAAAAGATACCAGATTCTGGGATTCGCAAGATGATACTTCATATGATTCAGTTGGAACCGGAGTTGCGACATTCTGCTGATAGCTACCTTCAGGAGTATACAACCATTGTTTTTCCAAGTTACTTCTCTCCATTTCTGCATAATTTCCATTGTTTCTGGAATCCCCTTCATTCAGATATGAGGGTTGCACTTTGCCATAGTGTTTTTCATGAAATACTTAAACAAATGATGAGCAGTAGGTCAACTGAGGACACTGGAACGGGAGTGGGAACTCCTTCAAGTGCCCATGGCATTAGTGGTAGAACTTCTCAGGAAGCAGTTGTAATGCAGAACAAGGGTTTGACAAAGGGATCGTTTACAAAGAAAGAAGAGATGGGTAAAGGCTTAAAATGTGATCAATTGGAACTTCTTGGCGATATCAATACCCTTCTTCGGGATGTTAAACAAAGTAACAATTATTCTGCTACCAAGCCAATCCTTAACGATAATCCCAATTCTGCATTTTCCCCGAATCTTGGCAACTATGGCATGCAATCTCCTGGTGAATTACTTCAATCTATCTCTAATGCATTTAGGAGAAACGATCATCCTTTCCTGAAAAAGATTACATTGAATGATTTGAATTCTTTGATGTCCAAGTATGACAGCGAATCGGATACCTTCGGCACACCGTTTCTGCCGTTACCTGAAAACAGTATCAGATGTGAGGGCATGGTTCTGATTACCTCGTTGCTCTGTTCATGCATACGTAATGTAAAATTGCCTCATTTAAGGAGGAGGGCCATACTTTTGCTGAAATCCTCTGCCTTGTATATTGATGATGAAGATCGATTGCAACGTGTAATTCCATATGTTGTTGCAATGCTTTCAGATGCAGCTGCAATTGTGCGTTGTGCTGCCTTGGAGACGTTGTGTGACATACTACCTCTAGTCCGAGATTTTCCTCCCAGTGATGCAAAAATTTTTCCGGAGTATATTCTGCCAATGCTTTCCATGCTTCCGGATCGATCCAGAAGAAAGTGTGAGGATATGTTATGCCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000011 GO:0000151 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005737 GO:0005768 GO:0005770 GO:0005942 GO:0006355 GO:0006357 GO:0006464 GO:0006468 GO:0006605 GO:0006623 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006886 GO:0006914 GO:0006996 GO:0007033 GO:0007034 GO:0007154 GO:0007165 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009555 GO:0009846 GO:0009856 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010008 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0016236 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019219 GO:0019222 GO:0019538 GO:0019898 GO:0022414 GO:0023052 GO:0030242 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031410 GO:0031461 GO:0031967 GO:0031975 GO:0031982 GO:0032182 GO:0032501 GO:0032502 GO:0032507 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0033036 GO:0033365 GO:0034067 GO:0034243 GO:0034271 GO:0034272 GO:0034613 GO:0035032 GO:0035556 GO:0036211 GO:0042886 GO:0043130 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0044706 GO:0045053 GO:0045184 GO:0045185 GO:0045324 GO:0045893 GO:0045935 GO:0045944 GO:0046907 GO:0048015 GO:0048017 GO:0048229 GO:0048308 GO:0048518 GO:0048522 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051235 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051651 GO:0051704 GO:0051716 GO:0060255 GO:0061695 GO:0061919 GO:0065007 GO:0065008 GO:0070727 GO:0071561 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0080008 GO:0080090 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0120095 GO:0140096 GO:1901564 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04136 ko04138 ko04140 ko04371 map04136 map04138 map04140 map04371
Pfam Domains
Protein Families
HEAT Pkinase WD40

Protein Analysis

609

Amino Acids

69.74

Weight (kDa)

6.66

Isoelectric Point (pI)

48.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 31
Acc36I ACCTGC 1 cut(s) 622
AccB7I CCANNNNNTGG 1 cut(s) 800
AccI GTMKAC 1 cut(s) 881
AccII CGCG 2 cut(s) 177, 198
AccIII TCCGGA 2 cut(s) 1750, 1783
AciI CCGC 6 cut(s) 103, 109, 177, 196, 211, 352
AclWI GGATC 5 cut(s) 533, 761, 1141, 1785, 1794
AcsI RAATTY 2 cut(s) 1405, 1743
AcuI CTGAAG 3 cut(s) 107, 431, 857
AcyI GRCGYC 1 cut(s) 38
AfaI GTAC 2 cut(s) 47, 203
AfiI CCNNNNNNNGG 7 cut(s) 91, 160, 226, 800, 1215, 1216, 1298
AflIII ACRYGT 1 cut(s) 1621
AgeI ACCGGT 1 cut(s) 339
AgsI TTSAA 9 cut(s) 118, 172, 325, 514, 785, 1062, 1337, 1396, 1405
AjnI CCWGG 2 cut(s) 78, 1321
AjuI GAANNNNNNNTTGG 2 cut(s) 368, 400
AluBI AGCT 4 cut(s) 299, 700, 867, 1661
AluI AGCT 4 cut(s) 299, 700, 867, 1661
Alw21I GWGCWC 1 cut(s) 244
Alw26I GTCTC 2 cut(s) 278, 1682
AlwI GGATC 5 cut(s) 533, 761, 1141, 1785, 1794
AlwNI CAGNNNCTG 1 cut(s) 800
Aor13HI TCCGGA 2 cut(s) 1750, 1783
AoxI GGCC 4 cut(s) 263, 387, 656, 1563
ApeKI GCWGC 4 cut(s) 296, 1658, 1661, 1679
ApoI RAATTY 2 cut(s) 1405, 1743
AsiGI ACCGGT 1 cut(s) 339
Asp700I GAANNNNTTC 2 cut(s) 1057, 1332
AspLEI GCGC 2 cut(s) 177, 210
AspS9I GGNCC 4 cut(s) 82, 151, 264, 1563
AsuHPI GGTGA 6 cut(s) 191, 242, 455, 481, 635, 1337
AsuII TTCGAA 1 cut(s) 726
AvaII GGWCC 2 cut(s) 82, 151
BaeGI GKGCMC 1 cut(s) 1069
Bbv12I GWGCWC 1 cut(s) 244
BbvI GCAGC 4 cut(s) 308, 1648, 1666, 1670
BccI CCATC 3 cut(s) 73, 667, 1150
BceAI ACGGC 1 cut(s) 1447
BciT130I CCWGG 2 cut(s) 80, 1323
BciVI GTATCC 1 cut(s) 1432
BclI TGATCA 1 cut(s) 1177
BcoDI GTCTC 2 cut(s) 278, 1682
BfaI CTAG 3 cut(s) 98, 771, 1712
BfmI CTRYAG 1 cut(s) 678
BfoI RGCGCY 1 cut(s) 211
BfuAI ACCTGC 1 cut(s) 622
BfuI GTATCC 1 cut(s) 1432
BisI GCNGC 6 cut(s) 109, 211, 297, 1659, 1662, 1680
BlpI GCTNAGC 1 cut(s) 696
BlsI GCNGC 6 cut(s) 110, 212, 298, 1660, 1663, 1681
BmcAI AGTACT 1 cut(s) 47
Bme1390I CCNGG 2 cut(s) 80, 1323
Bme18I GGWCC 2 cut(s) 82, 151
BmgT120I GGNCC 4 cut(s) 82, 151, 264, 1563
BmiI GGNNCC 2 cut(s) 107, 840
BmrFI CCNGG 2 cut(s) 80, 1323
BmrI ACTGGG 1 cut(s) 1725
BmsI GCATC 5 cut(s) 515, 618, 634, 1645, 1726
BmuI ACTGGG 1 cut(s) 1725
Bpu1102I GCTNAGC 1 cut(s) 696
Bpu14I TTCGAA 1 cut(s) 726
BpuEI CTTGAG 1 cut(s) 728
Bsa29I ATCGAT 2 cut(s) 1612, 1789
BsaAI YACGTR 1 cut(s) 1532
BsaBI GATNNNNATC 1 cut(s) 1608
BsaHI GRCGYC 1 cut(s) 38
BsaJI CCNNGG 2 cut(s) 1069, 1683
BsaWI WCCGGW 5 cut(s) 339, 569, 841, 1750, 1783
BsaXI ACNNNNNCTCC 2 cut(s) 1679, 1709
Bsc4I CCNNNNNNNGG 7 cut(s) 91, 160, 226, 800, 1215, 1216, 1298
Bse118I RCCGGY 1 cut(s) 339
Bse1I ACTGG 4 cut(s) 271, 1042, 1731, 1822
Bse3DI GCAATG 1 cut(s) 1650
Bse8I GATNNNNATC 1 cut(s) 1608
BseAI TCCGGA 2 cut(s) 1750, 1783
BseBI CCWGG 2 cut(s) 80, 1323
BseCI ATCGAT 2 cut(s) 1612, 1789
BseDI CCNNGG 2 cut(s) 1069, 1683
BseGI GGATG 3 cut(s) 689, 1225, 1369
BseJI GATNNNNATC 1 cut(s) 1608
BseLI CCNNNNNNNGG 7 cut(s) 91, 160, 226, 800, 1215, 1216, 1298
BseMI GCAATG 1 cut(s) 1650
BseMII CTCAG 5 cut(s) 376, 591, 687, 1020, 1106
BseNI ACTGG 4 cut(s) 271, 1042, 1731, 1822
BseRI GAGGAG 1 cut(s) 1573
BseSI GKGCMC 1 cut(s) 1069
BseXI GCAGC 4 cut(s) 308, 1648, 1666, 1670
BsgI GTGCAG 1 cut(s) 317
Bsh1236I CGCG 2 cut(s) 177, 198
Bsh1285I CGRYCG 1 cut(s) 340
BshFI GGCC 4 cut(s) 265, 389, 658, 1565
BshTI ACCGGT 1 cut(s) 339
BshVI ATCGAT 2 cut(s) 1612, 1789
BsiEI CGRYCG 1 cut(s) 340
BsiHKAI GWGCWC 1 cut(s) 244
BsiSI CCGG 5 cut(s) 340, 570, 842, 1751, 1784
BslFI GGGAC 1 cut(s) 726
BslI CCNNNNNNNGG 7 cut(s) 91, 160, 226, 800, 1215, 1216, 1298
BsmAI GTCTC 2 cut(s) 278, 1682
BsmBI CGTCTC 1 cut(s) 1682
BsmFI GGGAC 1 cut(s) 726
BsnI GGCC 4 cut(s) 265, 389, 658, 1565
Bsp119I TTCGAA 1 cut(s) 726
Bsp1286I GDGCHC 2 cut(s) 244, 1069
Bsp13I TCCGGA 2 cut(s) 1750, 1783
Bsp1720I GCTNAGC 1 cut(s) 696
Bsp19I CCATGG 1 cut(s) 1069
BspACI CCGC 6 cut(s) 103, 109, 177, 196, 211, 352
BspANI GGCC 4 cut(s) 265, 389, 658, 1565
BspCNI CTCAG 5 cut(s) 375, 592, 688, 1021, 1105
BspDI ATCGAT 2 cut(s) 1612, 1789
BspEI TCCGGA 2 cut(s) 1750, 1783
BspFNI CGCG 2 cut(s) 177, 198
BspHI TCATGA 1 cut(s) 991
BspLI GGNNCC 2 cut(s) 107, 840
BspMI ACCTGC 1 cut(s) 622
BspPI GGATC 5 cut(s) 533, 761, 1141, 1785, 1794
BspT104I TTCGAA 1 cut(s) 726
BsrDI GCAATG 1 cut(s) 1650
BsrFI RCCGGY 1 cut(s) 339
BsrI ACTGG 4 cut(s) 271, 1042, 1731, 1822
BssAI RCCGGY 1 cut(s) 339
BssECI CCNNGG 2 cut(s) 1069, 1683
BssNAI GTATAC 1 cut(s) 882
BssNI GRCGYC 1 cut(s) 38
BssT1I CCWWGG 2 cut(s) 1069, 1683
Bst1107I GTATAC 1 cut(s) 882
Bst2UI CCWGG 2 cut(s) 80, 1323
Bst4CI ACNGT 4 cut(s) 443, 723, 1455, 1478
Bst6I CTCTTC 2 cut(s) 221, 1146
BstACI GRCGYC 1 cut(s) 38
BstAPI GCANNNNNTGC 1 cut(s) 644
BstBAI YACGTR 1 cut(s) 1532
BstBI TTCGAA 1 cut(s) 726
BstC8I GCNNGC 2 cut(s) 134, 1313
BstDEI CTNAG 5 cut(s) 362, 600, 696, 1029, 1092
BstDSI CCRYGG 1 cut(s) 1069
BstENI CCTNNNNNAGG 3 cut(s) 89, 158, 224
BstF5I GGATG 3 cut(s) 689, 1225, 1369
BstFNI CGCG 2 cut(s) 177, 198
BstH2I RGCGCY 1 cut(s) 211
BstHHI GCGC 2 cut(s) 177, 210
BstMAI GTCTC 2 cut(s) 278, 1682
BstMCI CGRYCG 1 cut(s) 340
BstMWI GCNNNNNNNGC 4 cut(s) 503, 644, 1308, 1777
BstNI CCWGG 2 cut(s) 80, 1323
BstNSI RCATGY 1 cut(s) 1315
BstSCI CCNGG 2 cut(s) 78, 1321
BstSFI CTRYAG 1 cut(s) 678
BstSLI GKGCMC 1 cut(s) 1069
BstSNI TACGTA 1 cut(s) 1532
BstUI CGCG 2 cut(s) 177, 198
BstV1I GCAGC 4 cut(s) 308, 1648, 1666, 1670
BstZ17I GTATAC 1 cut(s) 882
Bsu15I ATCGAT 2 cut(s) 1612, 1789
BsuI GTATCC 1 cut(s) 1432
BsuRI GGCC 4 cut(s) 265, 389, 658, 1565
BsuTUI ATCGAT 2 cut(s) 1612, 1789
BtgI CCRYGG 1 cut(s) 1069
BtsCI GGATG 3 cut(s) 689, 1225, 1369
BtsI GCAGTG 1 cut(s) 422
BtsIMutI CAGTG 4 cut(s) 422, 719, 1035, 1738
BveI ACCTGC 1 cut(s) 622
Cac8I GCNNGC 2 cut(s) 134, 1313
CaiI CAGNNNCTG 1 cut(s) 800
CciI TCATGA 1 cut(s) 991
CfoI GCGC 2 cut(s) 177, 210
Cfr10I RCCGGY 1 cut(s) 339
Cfr13I GGNCC 4 cut(s) 82, 151, 264, 1563
ClaI ATCGAT 2 cut(s) 1612, 1789
CseI GACGC 2 cut(s) 27, 34
CsiI ACCWGGT 1 cut(s) 78
Csp6I GTAC 2 cut(s) 46, 202
CspAI ACCGGT 1 cut(s) 339
CviAII CATG 8 cut(s) 422, 617, 992, 1070, 1312, 1495, 1524, 1777
CviQI GTAC 2 cut(s) 46, 202
DdeI CTNAG 5 cut(s) 362, 600, 696, 1029, 1092
DrdI GACNNNNNNGTC 1 cut(s) 31
DseDI GACNNNNNNGTC 1 cut(s) 31
Eam1104I CTCTTC 2 cut(s) 221, 1146
EarI CTCTTC 2 cut(s) 221, 1146
EciI GGCGGA 1 cut(s) 118
Eco105I TACGTA 1 cut(s) 1532
Eco130I CCWWGG 2 cut(s) 1069, 1683
Eco32I GATATC 1 cut(s) 1201
Eco47I GGWCC 2 cut(s) 82, 151
Eco57I CTGAAG 3 cut(s) 107, 431, 857
EcoNI CCTNNNNNAGG 3 cut(s) 89, 158, 224
EcoRI GAATTC 1 cut(s) 1405
EcoRII CCWGG 2 cut(s) 78, 1321
EcoRV GATATC 1 cut(s) 1201
EcoT14I CCWWGG 2 cut(s) 1069, 1683
EcoT22I ATGCAT 2 cut(s) 1354, 1529
ErhI CCWWGG 2 cut(s) 1069, 1683
Esp3I CGTCTC 1 cut(s) 1682
FaeI CATG 8 cut(s) 425, 620, 995, 1073, 1315, 1498, 1527, 1780
FalI AAGNNNNNCTT 2 cut(s) 218, 250
FaqI GGGAC 1 cut(s) 726
FatI CATG 8 cut(s) 421, 616, 991, 1069, 1311, 1494, 1523, 1776
FauI CCCGC 1 cut(s) 359
FauNDI CATATG 3 cut(s) 532, 825, 1633
FbaI TGATCA 1 cut(s) 1177
FblI GTMKAC 1 cut(s) 881
Fnu4HI GCNGC 6 cut(s) 109, 211, 297, 1659, 1662, 1680
FokI GGATG 3 cut(s) 696, 1232, 1356
Fsp4HI GCNGC 6 cut(s) 109, 211, 297, 1659, 1662, 1680
FspBI CTAG 3 cut(s) 98, 771, 1712
GlaI GCGC 2 cut(s) 176, 209
GluI GCNGC 6 cut(s) 109, 211, 297, 1659, 1662, 1680
HaeII RGCGCY 1 cut(s) 211
HaeIII GGCC 4 cut(s) 265, 389, 658, 1565
HapII CCGG 5 cut(s) 340, 570, 842, 1751, 1784
HgaI GACGC 2 cut(s) 27, 34
HhaI GCGC 2 cut(s) 177, 210
Hin1I GRCGYC 1 cut(s) 38
Hin1II CATG 8 cut(s) 425, 620, 995, 1073, 1315, 1498, 1527, 1780
Hin6I GCGC 2 cut(s) 175, 208
HinP1I GCGC 2 cut(s) 175, 208
HincII GTYRAC 1 cut(s) 1026
HindII GTYRAC 1 cut(s) 1026
HinfI GANTC 7 cut(s) 182, 797, 805, 829, 943, 1294, 1433
HpaII CCGG 5 cut(s) 340, 570, 842, 1751, 1784
HphI GGTGA 6 cut(s) 191, 242, 455, 481, 635, 1337
Hpy166II GTNNAC 3 cut(s) 882, 1026, 1140
Hpy188I TCNGA 9 cut(s) 43, 411, 547, 958, 1438, 1484, 1503, 1654, 1718
Hpy8I GTNNAC 3 cut(s) 882, 1026, 1140
Hpy99I CGWCG 3 cut(s) 28, 43, 143
HpyCH4III ACNGT 4 cut(s) 443, 723, 1455, 1478
HpyCH4IV ACGT 4 cut(s) 258, 1531, 1621, 1691
HpyF10VI GCNNNNNNNGC 4 cut(s) 503, 644, 1308, 1777
HpyF3I CTNAG 5 cut(s) 362, 600, 696, 1029, 1092
HpySE526I ACGT 4 cut(s) 258, 1531, 1621, 1691
Hsp92I GRCGYC 1 cut(s) 38
Hsp92II CATG 8 cut(s) 425, 620, 995, 1073, 1315, 1498, 1527, 1780
HspAI GCGC 2 cut(s) 175, 208
Kpn2I TCCGGA 2 cut(s) 1750, 1783
Ksp22I TGATCA 1 cut(s) 1177
LmnI GCTCC 2 cut(s) 105, 601
Lsp1109I GCAGC 4 cut(s) 308, 1648, 1666, 1670
LweI GCATC 5 cut(s) 515, 618, 634, 1645, 1726
MabI ACCWGGT 1 cut(s) 78
MaeI CTAG 3 cut(s) 98, 771, 1712
MaeII ACGT 4 cut(s) 258, 1531, 1621, 1691
MaeIII GTNAC 8 cut(s) 179, 396, 437, 469, 902, 1232, 1464, 1697
MboII GAAGA 9 cut(s) 106, 208, 313, 388, 588, 1163, 1205, 1619, 1809
MfeI CAATTG 2 cut(s) 1181, 1665
MhlI GDGCHC 2 cut(s) 244, 1069
MlyI GAGTC 1 cut(s) 176
MmeI TCCRAC 2 cut(s) 21, 816
Mph1103I ATGCAT 2 cut(s) 1354, 1529
MroI TCCGGA 2 cut(s) 1750, 1783
MroXI GAANNNNTTC 2 cut(s) 1057, 1332
MseI TTAA 8 cut(s) 344, 450, 653, 1002, 1169, 1224, 1263, 1553
MslI CAYNNNNRTG 1 cut(s) 621
MspA1I CMGCKG 1 cut(s) 1661
MspI CCGG 5 cut(s) 340, 570, 842, 1751, 1784
MspR9I CCNGG 2 cut(s) 80, 1323
MunI CAATTG 2 cut(s) 1181, 1665
MvaI CCWGG 2 cut(s) 80, 1323
MvnI CGCG 2 cut(s) 177, 198
MwoI GCNNNNNNNGC 4 cut(s) 503, 644, 1308, 1777
NcoI CCATGG 1 cut(s) 1069
NdeI CATATG 3 cut(s) 532, 825, 1633
NlaIII CATG 8 cut(s) 425, 620, 995, 1073, 1315, 1498, 1527, 1780
NlaIV GGNNCC 2 cut(s) 107, 840
NmuCI GTSAC 4 cut(s) 179, 437, 469, 1697
NsiI ATGCAT 2 cut(s) 1354, 1529
NspI RCATGY 1 cut(s) 1315
NspV TTCGAA 1 cut(s) 726
PaeI GCATGC 1 cut(s) 1315
PagI TCATGA 1 cut(s) 991
PcsI WCGNNNNNNNCGW 1 cut(s) 1618
PdmI GAANNNNTTC 2 cut(s) 1057, 1332
PfeI GAWTC 6 cut(s) 797, 805, 829, 943, 1294, 1433
PflMI CCANNNNNTGG 1 cut(s) 800
PinAI ACCGGT 1 cut(s) 339
PkrI GCNGC 6 cut(s) 110, 212, 298, 1660, 1663, 1681
PleI GAGTC 1 cut(s) 176
PpsI GAGTC 1 cut(s) 176
Ppu21I YACGTR 1 cut(s) 1532
Psp6I CCWGG 2 cut(s) 78, 1321
PspGI CCWGG 2 cut(s) 78, 1321
PspN4I GGNNCC 2 cut(s) 107, 840
PspPI GGNCC 4 cut(s) 82, 151, 264, 1563
PstNI CAGNNNCTG 1 cut(s) 800
PvuII CAGCTG 1 cut(s) 1661
RsaI GTAC 2 cut(s) 47, 203
RsaNI GTAC 2 cut(s) 46, 202
RseI CAYNNNNRTG 1 cut(s) 621
SaqAI TTAA 8 cut(s) 344, 450, 653, 1002, 1169, 1224, 1263, 1553
SatI GCNGC 6 cut(s) 109, 211, 297, 1659, 1662, 1680
Sau96I GGNCC 4 cut(s) 82, 151, 264, 1563
ScaI AGTACT 1 cut(s) 47
SchI GAGTC 1 cut(s) 176
ScrFI CCNGG 2 cut(s) 80, 1323
SduI GDGCHC 2 cut(s) 244, 1069
SexAI ACCWGGT 1 cut(s) 78
SfaNI GCATC 5 cut(s) 515, 618, 634, 1645, 1726
SfcI CTRYAG 1 cut(s) 678
SfuI TTCGAA 1 cut(s) 726
SinI GGWCC 2 cut(s) 82, 151
SmiMI CAYNNNNRTG 1 cut(s) 621
SmlI CTYRAG 1 cut(s) 707
SmoI CTYRAG 1 cut(s) 707
SnaBI TACGTA 1 cut(s) 1532
SphI GCATGC 1 cut(s) 1315
SsiI CCGC 6 cut(s) 103, 109, 177, 196, 211, 352
SspMI CTAG 3 cut(s) 98, 771, 1712
StyD4I CCNGG 2 cut(s) 78, 1321
StyI CCWWGG 2 cut(s) 1069, 1683
TaaI ACNGT 4 cut(s) 443, 723, 1455, 1478
TaiI ACGT 4 cut(s) 261, 1534, 1624, 1694
TaqI TCGA 7 cut(s) 19, 122, 246, 372, 726, 1612, 1789
TatI WGTACW 1 cut(s) 45
TauI GCSGC 2 cut(s) 111, 213
TfiI GAWTC 6 cut(s) 797, 805, 829, 943, 1294, 1433
Tru1I TTAA 8 cut(s) 344, 450, 653, 1002, 1169, 1224, 1263, 1553
Tru9I TTAA 8 cut(s) 344, 450, 653, 1002, 1169, 1224, 1263, 1553
TscAI CASTG 4 cut(s) 422, 726, 1042, 1738
TseFI GTSAC 4 cut(s) 179, 437, 469, 1697
TseI GCWGC 4 cut(s) 296, 1658, 1661, 1679
Tsp45I GTSAC 4 cut(s) 179, 437, 469, 1697
TspDTI ATGAA 6 cut(s) 812, 941, 980, 1008, 1512, 1620
TspGWI ACGGA 1 cut(s) 227
TspRI CASTG 4 cut(s) 422, 726, 1042, 1738
Van91I CCANNNNNTGG 1 cut(s) 800
VpaK11BI GGWCC 2 cut(s) 82, 151
XagI CCTNNNNNAGG 3 cut(s) 89, 158, 224
XapI RAATTY 2 cut(s) 1405, 1743
XceI RCATGY 1 cut(s) 1315
XcmI CCANNNNNNNNNTGG 1 cut(s) 1076
XmiI GTMKAC 1 cut(s) 881
XmnI GAANNNNTTC 2 cut(s) 1057, 1332
XspI CTAG 3 cut(s) 98, 771, 1712
ZrmI AGTACT 1 cut(s) 47
Zsp2I ATGCAT 2 cut(s) 1354, 1529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.