Rroxscaffold_4G00319050

Phosphoinositide 3-kinase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001323
Physical Location & Seq
Reverse (-)
46528987 .. 46542362
13376 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00319050.1

Sequence Viewer

Length: 4473 bp
ATGGCGGTGGTTATTCTAGGTCGTTGGGATCTATATGGAACTCGATCTCGTGGTGTCGGTACTCAACAGTGGGTGTCGGTGCTAGGGGTCGAGTCGCAAACCCTAACCCTAACCTTTCTCAAAATGGGATTAAGGGTTCCGGTTGGGCTCGTGCTTGGGCTTGGGTTTAATAAATCACTCACCGGTGGGCGCAGTAGTGCGGTGGCGCCGGCTTTCACTCCCATTTGCTCTCTAACCGGTTTCATCATGTCTCGCTTGGGATATCCGATGCGGAGTCGACTCAAATTTGGCACGGTTTCGTTTGACGATGCGCTTGGTTGTGCCTCCTCCCCGTGCGGCGTCCGGTGGAGCATGCACCGATTAGTGGAGTCAAACATAGCTGATTCCATGGCTATGATCACTTATAGCAAAAAATCTACGGCTACTTCCCTTGTGGAGTTAGGGTTTACGTCAGAGCTGGTTATCCCAAATCAGGCTGACAAGCTTCTTGTTTCACCTCCCAAAGAAATGCAAAAGGTGATTGATTGGGAGAGGGAAGGAGTAGGTATGGGGAACAAGATCGCGCGGACGACCCAGGTGTCGGCGTCGGAGTATTACCTGCACGACCTGCCGTCCTCCTACAACCTCGTGCTGAAGGAGGTTTTGGGTCGCGGACGCTTCTTCAAGTCGATTCAGTGCAAGCACGACGAGGGTTTGGTCCTCGTCAAGGTCTATTTCAAGCGCGGCGACTCCATCGATCTCCGCGAGTACGAGCGCCGGCTCTTCCATATCAAGGAGACCTTTCGCGCCCTCGATCACCCTCACGTCTGGCCCTTCCAGTTTTGGCAAGAGACGGATAAAGCAGCTTATCTTGTGAGACAATACTTCTTCAATAATCTACATGATCGATTGAGCACGCGCCCCTTTCTCAGTCTCATCGAAAAGAAATGGTTGGCTTTTCAGTTACTTCTTGCTGTGAAACAGTGCCACGACAAAGGAATATGTCACGGTGATATTAAGTGCGAGAATGTCCTGGTGACTTCTTGGAATTGGCTCTATCTTGCTGACTTTGCTTCCTTCAAACCCACTTACATTCCGTATGATGACCCTTCTGATTTCTCATTTTTCTACGACACCGGAGGAAGAAGGCTTTGTTATCTTGCTCCCGAGAGATTTTATGAGCATGGAGGTGAGATGCAAGTTGCACAAGATGCACCATTAAGACCATCTATGGATATCTTTGCTGTAGGGTGTGTGATTGCTGAGCTTTTTCTTGAGGGTCAGCCACTATTTGAACTCTCTCAACTTCTCGCTTATCGTAGAGGGCAATACGATCCTAGTCAACTTCTTGAAAAAGGTTCTCTCTCTCTACTTTGTCCTCTTGATGATGTTGCCCTCTTAATCTTGAGCATTATATTGCACATGACTTTTGTTCTACATAACTGTTTACTGGTTTTACGTGATTATATACCAGATTGTGGAATCCGCAAAATGATACTTCATATGATTCAGTTGGAACCGGAGTTGCGACTTTCTGCTGATAGCTACCTGCAGGAGGTTGCACTTTGCCAGAGTGTTTTTCCTGAGATACTGAAACAAATGATGAGCAATAGGTCAACTCAGGACACTAGCACTGGACATGGAACTCCTTCAAACATCCATGCTGTTAGCGGTAAATCTTCTCAGGACATGATTGCAATGCAGAACATGAATTTGACAAAGGGCTCACCGGGAAACAAAGTAGAGATGGATAAAGGTTTCAAGCGCGATCAATTTGAACTTCTCGGTGATATCAATACGCTTCTTAGGGATGTTAAACAGAGCAATCATTATTCTAATATGAAGCCAATGGTGGAGGACAACACTAATTCTGCATTTTCCCAGAATCTTGGAAACTATGGCATGCAATCTCCTGGTGAATTACTTCAGACTATCTCCCATGCATTTAGGAGAAACGATCATCCTTTTCTAAAAAAGATTACAATGAATGATCTGAATTCATTGATGTCCAAGTATGACAGCCAGTCGGATACCTTTGAGGAAAGTGTGAGGATATGTTATGCCAGTAATATAGCTAAGCTGGCACTAACTGCTTATGGCTTTTTAGTTCACTCAATAACCTTGAGTGAGGCTGGTGTGCTTGATGAATTAAGTTCTCCAAAGAAGCTACTGGCATCATCTAGTGAGACATCTGGTCAGCTCCACAAGCAAACTCCCAATATCAGGAGAGCACTCCTTCAGGATATCAGCAATCTATGCTGCTTCTTTGGCCAGAGGCAGAGTAATGACTTTTTGTTACCCATTCTCCCTGCTTTTCTGAATGATCGAGATGAGCAGCTAAGAGCAGTATTCTATGGGCAAATTGTGTATGTATGCTTCTTTGTGGGTCAAAGAAGTGTGGAAGAATATCTTTTACCTTATATTGAGCAGGCTGTAAGTGATGCTACAGAGGCTGTTATTGTCAATGCATTGGACTGTCTGGCCATTTTGTGCAAAAGTGGTTACCTGCGCAAGAGGATACTCCTGGAAATGATTGAGCGTTCATTTCCATTGTTATGCTATCCTAGTCAATGGCCAGCATCTCTAGCTTCTGAGAAGGCTCTCCTTTCATGTTTGAAGCCTCCTGTCTCAAGACAGGTATTTTACCAAGTGCTAGAAAATGCAAGGAGTTCAGACATGTTGGAAAGACAGAGAAAGATATGGTACAATTCTTGGCCTCAATCTAAACAATGGGAAAGTGTGGATTTACTTCATAAAGGGGTTGGCGAATTGAATTCGATGAGGAGCTGGACAGACAACCAAGAAAGTCCTGAGGGTCAAAAACATTCTGGAAATGAATTGCAACAGGGAAAGCTAACTGAATGTGATGATGGTGAGGCTAAGTTTGGATGTATGGGAAGCTTTACGCGCAATGCTTCGAGCACAGTTGACATTCATGATCCTTTATCCTCTGAAAAGTTGCAGTATTCGGGCTTTATGTGGCCACAGGGAAGTACTGTGAATAGCTTCATGTGTGATAAATCATCAGTAGGGATACCTTTGTACTCATTTAGCATGGACAGGCGAGCAGTGGGAGTTCCTCCTGCTCCATCTGACTCTTCATTACAAGTGAGTTCTGTAGGAGTTGGTGCATCATCTATGCCATGGATGGATCCAGTAAATAAATCTTTTAGTTTAGCTAGTTCTGTTCCAGCACCCAAGCTCGTGTCAGGATCATTCAACATCAGCAGTGGTTCTAAACAATTCTACAGAGTAGTACATGAACCTGATGGTAGGGATAATGACCAAACAACCTTTGTCAATAGCAAATTTCAAGATATGGGGTTATCTAGTGCTACAAAAGCAAGTTCTATTACTGTGGAAGATGCATCCTCTACAAGTGATCTAACAGGATTGCCCTCTTCTACAAGAGCTTCGTCAATTCCAGATTCTGGTTGGAGGCCTCGTGGGGTTTTGGTTGCACACTTACAAGAGCACCGGTCTGCTGTGAATGACATAGCCGTATCAACTGATCATAGCTTCTTTGTGAGTGCATCTGATGATTCCACTGTCAAGGTATGGGATTCGAGAAAGTTGGAAAAGGACATCTCATTTAGGTCAAGATTGACTTATCATCTGGAGGGAAGCCGTGCACTATGTTCTGTAATGCTCCGGGGCTGCGCTCAAGTTGTAGTTGGAGCATGTGATGGCATGATACATATGTTTTCTGTTGATTACATTTCCAGAGGTCTCGGTAATGTAGTTGAGAAGTATTCAGGATCTTCAAGGGGTGTGCTCACTCTTTGGGATATGAGGTTCCTTATTCCTGTGAATACATGGCAGTATTCTGCTGTTTGCCCCATAGAGAAAATGTGTCTTTTCCTTCCTCCTCCAAATGCTTCTGTTTCTGCTGCTGCAAGGCCCCTTGTTTATGTTGCTGCAGGTTGCAATGAAGTTTCTCTTTGGAATGCAGAAAATGGCACCTGCCACCAGGTGTTGAGGGTTGCGAGTTATGAAAGTGATGCTGAAATGTCTGAGGTGCCTTGGGCCTTGGCTCGATCATCAACTAAAAATTCCAAAGCGGATATGAGACGGAATGTCAATCCTCATTACAGAGTTGATGAACTGAATGAACCACCTCCTCGCATTCCTGGCATCCGTTCATTGCTTCCCTTACCTGGGGGTGATTTGTTAACTGGGGGAACCGACTTGAAGATTCGTCGATGGGATCATTACAGCCCTGATCGAAGTTACTGTATTTGTGGGCCAAACTTGAAGGGTGTTGGAAATGATGATTTCTATGGAACGCGGTCTAGCTTTGGCGTGCAAGTTGTGCAGGAGACAAAGAGACGGCCTCTAACAACAAAGTTGACAGCCAAGGCAGTTCTTGCAGCTGCTGCCACTGATACTGCTGGCTGCCATCGTGATTCAATCCTTTCATTGGCCTCTGTTAAGTTGAATCAAAGACACTTAATATCAAGCAGTAGAGATGGGGCAATCAAGGTCTGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000011 GO:0000151 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005737 GO:0005768 GO:0005770 GO:0005942 GO:0006355 GO:0006357 GO:0006464 GO:0006468 GO:0006605 GO:0006623 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006886 GO:0006914 GO:0006996 GO:0007033 GO:0007034 GO:0007154 GO:0007165 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009555 GO:0009846 GO:0009856 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010008 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0016236 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019219 GO:0019222 GO:0019538 GO:0019898 GO:0022414 GO:0023052 GO:0030242 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031410 GO:0031461 GO:0031967 GO:0031975 GO:0031982 GO:0032182 GO:0032501 GO:0032502 GO:0032507 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0033036 GO:0033365 GO:0034067 GO:0034243 GO:0034271 GO:0034272 GO:0034613 GO:0035032 GO:0035556 GO:0036211 GO:0042886 GO:0043130 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0044706 GO:0045053 GO:0045184 GO:0045185 GO:0045324 GO:0045893 GO:0045935 GO:0045944 GO:0046907 GO:0048015 GO:0048017 GO:0048229 GO:0048308 GO:0048518 GO:0048522 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051235 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051651 GO:0051704 GO:0051716 GO:0060255 GO:0061695 GO:0061919 GO:0065007 GO:0065008 GO:0070727 GO:0071561 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0080008 GO:0080090 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0120095 GO:0140096 GO:1901564 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04136 ko04138 ko04140 ko04371 map04136 map04138 map04140 map04371
Pfam Domains
Protein Families
HEAT Pkinase WD40

Protein Analysis

1490

Amino Acids

166.15

Weight (kDa)

7.65

Isoelectric Point (pI)

51.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 210 - 421 1.8e-07 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 211 - 511 8.5e-21 Protein kinase domain
VPS15-like_hel PF22956 669 - 851 1.8e-53 VPS15-like, helical domain
Beta-prop_WDR5 PF25175 1143 - 1230 3e-08 WDR5 beta-propeller domain
WD40 PF00400 1144 - 1181 1.3e-08 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3950
AasI GACNNNNNNGTC 1 cut(s) 577
Acc16I TGCGCA 1 cut(s) 2489
Acc36I ACCTGC 6 cut(s) 606, 615, 1536, 2493, 3890, 3950
AccB1I GGYRCC 3 cut(s) 205, 3938, 3997
AccB7I CCANNNNNTGG 2 cut(s) 1457, 3410
AccI GTMKAC 1 cut(s) 277
AclWI GGATC 8 cut(s) 36, 1307, 2912, 3125, 3138, 3199, 3745, 4194
AcoI YGGCCR 4 cut(s) 2248, 2460, 2552, 2960
AcsI RAATTY 6 cut(s) 284, 1690, 1975, 2752, 3287, 4030
AcuI CTGAAG 3 cut(s) 653, 1889, 2201
AcyI GRCGYC 3 cut(s) 206, 339, 584
AdeI CACNNNGTG 1 cut(s) 3952
AfaI GTAC 6 cut(s) 61, 749, 2684, 2974, 3023, 3237
AflIII ACRYGT 1 cut(s) 2655
AgeI ACCGGT 3 cut(s) 182, 236, 3456
AhdI GACNNNNNGTC 4 cut(s) 610, 2002, 2172, 4055
AjiI CACGTC 1 cut(s) 805
AjnI CCWGG 7 cut(s) 573, 1011, 1891, 2502, 3948, 4108, 4135
AjuI GAANNNNNNNTTGG 2 cut(s) 626, 658
AloI GAACNNNNNNTCC 4 cut(s) 120, 152, 3758, 3790
Alw21I GWGCWC 6 cut(s) 896, 2212, 2903, 3456, 3613, 3756
Alw44I GTGCAC 1 cut(s) 3609
AlwI GGATC 8 cut(s) 36, 1307, 2912, 3125, 3138, 3199, 3745, 4194
AlwNI CAGNNNCTG 3 cut(s) 2432, 3410, 4355
Ama87I CYCGRG 1 cut(s) 1145
ApaLI GTGCAC 1 cut(s) 3609
ApoI RAATTY 6 cut(s) 284, 1690, 1975, 2752, 3287, 4030
AsiGI ACCGGT 3 cut(s) 182, 236, 3456
Asp700I GAANNNNTTC 3 cut(s) 1627, 1902, 2984
AspS9I GGNCC 5 cut(s) 697, 810, 3879, 4005, 4223
AsuC2I CCSGG 2 cut(s) 1710, 3632
AvaI CYCGRG 1 cut(s) 1145
AvaII GGWCC 1 cut(s) 697
AxyI CCTNAGG 1 cut(s) 2790
BaeGI GKGCMC 1 cut(s) 3613
BaeI ACNNNNGTAYC 4 cut(s) 3005, 3038, 3665, 3698
BalI TGGCCA 4 cut(s) 2250, 2462, 2554, 2962
BamHI GGATCC 1 cut(s) 3130
BanI GGYRCC 3 cut(s) 205, 3938, 3997
BanII GRGCYC 2 cut(s) 150, 1706
BarI GAAGNNNNNNTAC 2 cut(s) 2339, 2371
BauI CACGAG 5 cut(s) 48, 149, 626, 3182, 3423
Bbv12I GWGCWC 6 cut(s) 896, 2212, 2903, 3456, 3613, 3756
BceAI ACGGC 5 cut(s) 435, 595, 3464, 3591, 4325
BciT130I CCWGG 7 cut(s) 575, 1013, 1893, 2504, 3950, 4110, 4137
BciVI GTATCC 3 cut(s) 2002, 2490, 3006
BclI TGATCA 2 cut(s) 396, 3490
BcnI CCSGG 2 cut(s) 1710, 3632
BfmI CTRYAG 6 cut(s) 1224, 1529, 2424, 3096, 3226, 3897
BfoI RGCGCY 2 cut(s) 209, 757
BfuAI ACCTGC 6 cut(s) 606, 615, 1536, 2493, 3890, 3950
BfuI GTATCC 3 cut(s) 2002, 2490, 3006
BlpI GCTNAGC 2 cut(s) 1242, 2055
BmcAI AGTACT 1 cut(s) 2974
Bme1390I CCNGG 9 cut(s) 575, 1013, 1710, 1893, 2504, 3632, 3950, 4110, 4137
Bme18I GGWCC 1 cut(s) 697
BmeRI GACNNNNNGTC 4 cut(s) 610, 2002, 2172, 4055
BmeT110I CYCGRG 1 cut(s) 1145
BmgBI CACGTC 1 cut(s) 805
BmgT120I GGNCC 5 cut(s) 697, 810, 3879, 4005, 4223
BmiI GGNNCC 9 cut(s) 138, 207, 1497, 3132, 3776, 3881, 3940, 3999, 4162
BmrFI CCNGG 9 cut(s) 575, 1013, 1710, 1893, 2504, 3632, 3950, 4110, 4137
BmrI ACTGGG 1 cut(s) 4164
BmuI ACTGGG 1 cut(s) 4164
BplI GAGNNNNNCTC 2 cut(s) 4297, 4329
BpmI CTGGAG 1 cut(s) 3617
Bpu1102I GCTNAGC 2 cut(s) 1242, 2055
BpuEI CTTGAG 5 cut(s) 1274, 1405, 2122, 2593, 3627
BpuMI CCSGG 2 cut(s) 1710, 3632
Bsa29I ATCGAT 2 cut(s) 735, 886
BsaAI YACGTR 1 cut(s) 1439
BsaHI GRCGYC 3 cut(s) 206, 339, 584
BsaI GGTCTC 2 cut(s) 770, 3713
BsaJI CCNNGG 8 cut(s) 387, 573, 3122, 3631, 4001, 4008, 4136, 4335
BsaWI WCCGGW 7 cut(s) 139, 182, 236, 342, 1115, 1498, 3456
BsaXI ACNNNNNCTCC 6 cut(s) 428, 458, 2268, 2298, 3409, 3439
Bse118I RCCGGY 5 cut(s) 182, 208, 236, 756, 3456
Bse1I ACTGG 8 cut(s) 817, 1434, 1618, 2002, 2043, 2154, 3134, 4159
Bse21I CCTNAGG 1 cut(s) 2790
Bse3DI GCAATG 4 cut(s) 1683, 2896, 3913, 4121
BseBI CCWGG 7 cut(s) 575, 1013, 1893, 2504, 3950, 4110, 4137
BseCI ATCGAT 2 cut(s) 735, 886
BseDI CCNNGG 8 cut(s) 387, 573, 3122, 3631, 4001, 4008, 4136, 4335
BseGI GGATG 7 cut(s) 1635, 1795, 1939, 2873, 3132, 3347, 4113
BseMI GCAATG 4 cut(s) 1683, 2896, 3913, 4121
BseMII CTCAG 8 cut(s) 922, 1233, 1554, 1613, 1676, 2562, 2781, 3984
BseNI ACTGG 8 cut(s) 817, 1434, 1618, 2002, 2043, 2154, 3134, 4159
BseRI GAGGAG 4 cut(s) 316, 2776, 3837, 4089
BseSI GKGCMC 1 cut(s) 3613
BsgI GTGCAG 2 cut(s) 584, 4313
BshNI GGYRCC 3 cut(s) 205, 3938, 3997
BshTI ACCGGT 3 cut(s) 182, 236, 3456
BshVI ATCGAT 2 cut(s) 735, 886
BsiHKAI GWGCWC 6 cut(s) 896, 2212, 2903, 3456, 3613, 3756
BsiHKCI CYCGRG 1 cut(s) 1145
BsmBI CGTCTC 3 cut(s) 824, 4042, 4300
BsmI GAATGC 2 cut(s) 3931, 4104
Bso31I GGTCTC 2 cut(s) 770, 3713
BsoBI CYCGRG 1 cut(s) 1145
Bsp1286I GDGCHC 8 cut(s) 150, 896, 1706, 2212, 2903, 3456, 3613, 3756
Bsp1720I GCTNAGC 2 cut(s) 1242, 2055
Bsp19I CCATGG 2 cut(s) 387, 3122
BspCNI CTCAG 8 cut(s) 921, 1234, 1555, 1612, 1675, 2563, 2782, 3985
BspDI ATCGAT 2 cut(s) 735, 886
BspHI TCATGA 1 cut(s) 2914
BspLI GGNNCC 9 cut(s) 138, 207, 1497, 3132, 3776, 3881, 3940, 3999, 4162
BspMAI CTGCAG 2 cut(s) 1533, 3901
BspMI ACCTGC 6 cut(s) 606, 615, 1536, 2493, 3890, 3950
BspPI GGATC 8 cut(s) 36, 1307, 2912, 3125, 3138, 3199, 3745, 4194
BspQI GCTCTTC 1 cut(s) 767
BspT107I GGYRCC 3 cut(s) 205, 3938, 3997
BspTNI GGTCTC 2 cut(s) 770, 3713
BsrDI GCAATG 4 cut(s) 1683, 2896, 3913, 4121
BsrFI RCCGGY 5 cut(s) 182, 208, 236, 756, 3456
BsrI ACTGG 8 cut(s) 817, 1434, 1618, 2002, 2043, 2154, 3134, 4159
BssAI RCCGGY 5 cut(s) 182, 208, 236, 756, 3456
BssECI CCNNGG 8 cut(s) 387, 573, 3122, 3631, 4001, 4008, 4136, 4335
BssNI GRCGYC 3 cut(s) 206, 339, 584
BssSI CACGAG 5 cut(s) 48, 149, 626, 3182, 3423
BssT1I CCWWGG 5 cut(s) 387, 3122, 4001, 4008, 4335
Bst2BI CACGAG 5 cut(s) 48, 149, 626, 3182, 3423
Bst2UI CCWGG 7 cut(s) 575, 1013, 1893, 2504, 3950, 4110, 4137
Bst6I CTCTTC 3 cut(s) 767, 3082, 3385
BstACI GRCGYC 3 cut(s) 206, 339, 584
BstAPI GCANNNNNTGC 7 cut(s) 607, 1190, 2069, 2235, 4291, 4346, 4355
BstBAI YACGTR 1 cut(s) 1439
BstDSI CCRYGG 2 cut(s) 387, 3122
BstEII GGTNACC 1 cut(s) 2480
BstENI CCTNNNNNAGG 2 cut(s) 704, 1532
BstF5I GGATG 7 cut(s) 1635, 1795, 1939, 2873, 3132, 3347, 4113
BstH2I RGCGCY 2 cut(s) 209, 757
BstNI CCWGG 7 cut(s) 575, 1013, 1893, 2504, 3950, 4110, 4137
BstNSI RCATGY 4 cut(s) 355, 1885, 2659, 3663
BstPI GGTNACC 1 cut(s) 2480
BstSCI CCNGG 9 cut(s) 573, 1011, 1708, 1891, 2502, 3630, 3948, 4108, 4135
BstSFI CTRYAG 6 cut(s) 1224, 1529, 2424, 3096, 3226, 3897
BstSLI GKGCMC 1 cut(s) 3613
BstX2I RGATCY 3 cut(s) 28, 3130, 3737
BstXI CCANNNNNNTGG 1 cut(s) 1868
BstYI RGATCY 3 cut(s) 28, 3130, 3737
Bsu15I ATCGAT 2 cut(s) 735, 886
Bsu36I CCTNAGG 1 cut(s) 2790
BsuI GTATCC 3 cut(s) 2002, 2490, 3006
BsuTUI ATCGAT 2 cut(s) 735, 886
BtgI CCRYGG 2 cut(s) 387, 3122
BtrI CACGTC 1 cut(s) 805
BtsCI GGATG 7 cut(s) 1635, 1795, 1939, 2873, 3132, 3347, 4113
BtsI GCAGTG 2 cut(s) 3054, 3214
BtsIMutI CAGTG 8 cut(s) 74, 680, 968, 1611, 3054, 3214, 3525, 4359
BveI ACCTGC 6 cut(s) 606, 615, 1536, 2493, 3890, 3950
CaiI CAGNNNCTG 3 cut(s) 2432, 3410, 4355
CciI TCATGA 1 cut(s) 2914
Cfr10I RCCGGY 5 cut(s) 182, 208, 236, 756, 3456
Cfr13I GGNCC 5 cut(s) 697, 810, 3879, 4005, 4223
ClaI ATCGAT 2 cut(s) 735, 886
CseI GACGC 3 cut(s) 328, 573, 663
CsiI ACCWGGT 1 cut(s) 3948
Csp6I GTAC 6 cut(s) 60, 748, 2683, 2973, 3022, 3236
CspAI ACCGGT 3 cut(s) 182, 236, 3456
CviQI GTAC 6 cut(s) 60, 748, 2683, 2973, 3022, 3236
DinI GGCGCC 1 cut(s) 207
DraIII CACNNNGTG 1 cut(s) 3952
DrdI GACNNNNNNGTC 1 cut(s) 577
DriI GACNNNNNGTC 4 cut(s) 610, 2002, 2172, 4055
DseDI GACNNNNNNGTC 1 cut(s) 577
EaeI YGGCCR 4 cut(s) 2248, 2460, 2552, 2960
Eam1104I CTCTTC 3 cut(s) 767, 3082, 3385
Eam1105I GACNNNNNGTC 4 cut(s) 610, 2002, 2172, 4055
EarI CTCTTC 3 cut(s) 767, 3082, 3385
Eco130I CCWWGG 5 cut(s) 387, 3122, 4001, 4008, 4335
Eco147I AGGCCT 1 cut(s) 3421
Eco24I GRGCYC 2 cut(s) 150, 1706
Eco31I GGTCTC 2 cut(s) 770, 3713
Eco32I GATATC 4 cut(s) 263, 1216, 1771, 2224
Eco47I GGWCC 1 cut(s) 697
Eco57I CTGAAG 3 cut(s) 653, 1889, 2201
Eco81I CCTNAGG 1 cut(s) 2790
Eco88I CYCGRG 1 cut(s) 1145
Eco91I GGTNACC 1 cut(s) 2480
EcoNI CCTNNNNNAGG 2 cut(s) 704, 1532
EcoO109I RGGNCCY 1 cut(s) 3879
EcoO65I GGTNACC 1 cut(s) 2480
EcoRI GAATTC 2 cut(s) 1975, 2752
EcoRII CCWGG 7 cut(s) 573, 1011, 1891, 2502, 3948, 4108, 4135
EcoRV GATATC 4 cut(s) 263, 1216, 1771, 2224
EcoT14I CCWWGG 5 cut(s) 387, 3122, 4001, 4008, 4335
EcoT22I ATGCAT 3 cut(s) 1924, 2449, 3349
EcoT38I GRGCYC 2 cut(s) 150, 1706
EgeI GGCGCC 1 cut(s) 207
EheI GGCGCC 1 cut(s) 207
ErhI CCWWGG 5 cut(s) 387, 3122, 4001, 4008, 4335
Esp3I CGTCTC 3 cut(s) 824, 4042, 4300
FalI AAGNNNNNCTT 8 cut(s) 764, 796, 2373, 2405, 3571, 3603, 3903, 3935
FauNDI CATATG 2 cut(s) 1482, 3678
FbaI TGATCA 2 cut(s) 396, 3490
FblI GTMKAC 1 cut(s) 277
FokI GGATG 7 cut(s) 1622, 1802, 1926, 2880, 3139, 3334, 4100
FriOI GRGCYC 2 cut(s) 150, 1706
FspI TGCGCA 1 cut(s) 2489
GsuI CTGGAG 1 cut(s) 3617
HaeII RGCGCY 2 cut(s) 209, 757
HgaI GACGC 3 cut(s) 328, 573, 663
Hin1I GRCGYC 3 cut(s) 206, 339, 584
HincII GTYRAC 6 cut(s) 278, 1322, 1596, 2908, 4152, 4329
HindII GTYRAC 6 cut(s) 278, 1322, 1596, 2908, 4152, 4329
HindIII AAGCTT 2 cut(s) 482, 2878
HpaI GTTAAC 1 cut(s) 4152
Hpy99I CGWCG 3 cut(s) 589, 689, 4182
HpyCH4IV ACGT 3 cut(s) 449, 804, 1438
HpySE526I ACGT 3 cut(s) 449, 804, 1438
Hsp92I GRCGYC 3 cut(s) 206, 339, 584
KasI GGCGCC 1 cut(s) 205
KroI GCCGGC 2 cut(s) 208, 756
KroNI GCCGGC 2 cut(s) 210, 758
Ksp22I TGATCA 2 cut(s) 396, 3490
KspAI GTTAAC 1 cut(s) 4152
LguI GCTCTTC 1 cut(s) 767
LmnI GCTCC 7 cut(s) 348, 1147, 2184, 2763, 3070, 3633, 3656
MabI ACCWGGT 1 cut(s) 3948
MaeII ACGT 3 cut(s) 449, 804, 1438
MaeIII GTNAC 6 cut(s) 942, 983, 1015, 2274, 2480, 4208
MflI RGATCY 3 cut(s) 28, 3130, 3737
MhlI GDGCHC 8 cut(s) 150, 896, 1706, 2212, 2903, 3456, 3613, 3756
MlsI TGGCCA 4 cut(s) 2250, 2462, 2554, 2962
MluNI TGGCCA 4 cut(s) 2250, 2462, 2554, 2962
Mly113I GGCGCC 1 cut(s) 206
MlyI GAGTC 6 cut(s) 101, 273, 283, 377, 722, 3068
MmeI TCCRAC 8 cut(s) 567, 1473, 1986, 2640, 3395, 3534, 3634, 4222
Mox20I TGGCCA 4 cut(s) 2250, 2462, 2554, 2962
Mph1103I ATGCAT 3 cut(s) 1924, 2449, 3349
MroNI GCCGGC 2 cut(s) 208, 756
MroXI GAANNNNTTC 3 cut(s) 1627, 1902, 2984
MscI TGGCCA 4 cut(s) 2250, 2462, 2554, 2962
MslI CAYNNNNRTG 3 cut(s) 392, 1167, 2533
Msp20I TGGCCA 4 cut(s) 2250, 2462, 2554, 2962
MspA1I CMGCKG 1 cut(s) 4352
MspR9I CCNGG 9 cut(s) 575, 1013, 1710, 1893, 2504, 3632, 3950, 4110, 4137
Mva1269I GAATGC 2 cut(s) 3931, 4104
MvaI CCWGG 7 cut(s) 575, 1013, 1893, 2504, 3950, 4110, 4137
NaeI GCCGGC 2 cut(s) 210, 758
NarI GGCGCC 1 cut(s) 206
NciI CCSGG 2 cut(s) 1710, 3632
NcoI CCATGG 2 cut(s) 387, 3122
NdeI CATATG 2 cut(s) 1482, 3678
NgoMIV GCCGGC 2 cut(s) 208, 756
NlaIV GGNNCC 9 cut(s) 138, 207, 1497, 3132, 3776, 3881, 3940, 3999, 4162
NmuCI GTSAC 2 cut(s) 983, 1015
NsbI TGCGCA 1 cut(s) 2489
NsiI ATGCAT 3 cut(s) 1924, 2449, 3349
NspI RCATGY 4 cut(s) 355, 1885, 2659, 3663
PaeI GCATGC 2 cut(s) 355, 1885
PagI TCATGA 1 cut(s) 2914
PaqCI CACCTGC 1 cut(s) 3950
PceI AGGCCT 1 cut(s) 3421
PciI ACATGT 1 cut(s) 2655
PciSI GCTCTTC 1 cut(s) 767
PcsI WCGNNNNNNNCGW 1 cut(s) 741
PctI GAATGC 2 cut(s) 3931, 4104
PdiI GCCGGC 2 cut(s) 210, 758
PdmI GAANNNNTTC 3 cut(s) 1627, 1902, 2984
PflMI CCANNNNNTGG 2 cut(s) 1457, 3410
PfoI TCCNGGA 1 cut(s) 2502
PinAI ACCGGT 3 cut(s) 182, 236, 3456
PleI GAGTC 6 cut(s) 100, 273, 282, 376, 722, 3068
PluTI GGCGCC 1 cut(s) 209
PpsI GAGTC 6 cut(s) 100, 273, 282, 376, 722, 3068
Ppu21I YACGTR 1 cut(s) 1439
PscI ACATGT 1 cut(s) 2655
Psp6I CCWGG 7 cut(s) 573, 1011, 1891, 2502, 3948, 4108, 4135
PspEI GGTNACC 1 cut(s) 2480
PspGI CCWGG 7 cut(s) 573, 1011, 1891, 2502, 3948, 4108, 4135
PspN4I GGNNCC 9 cut(s) 138, 207, 1497, 3132, 3776, 3881, 3940, 3999, 4162
PspPI GGNCC 5 cut(s) 697, 810, 3879, 4005, 4223
PstI CTGCAG 2 cut(s) 1533, 3901
PstNI CAGNNNCTG 3 cut(s) 2432, 3410, 4355
PsuI RGATCY 3 cut(s) 28, 3130, 3737
PvuII CAGCTG 1 cut(s) 4352
RsaI GTAC 6 cut(s) 61, 749, 2684, 2974, 3023, 3237
RsaNI GTAC 6 cut(s) 60, 748, 2683, 2973, 3022, 3236
RseI CAYNNNNRTG 3 cut(s) 392, 1167, 2533
SalI GTCGAC 1 cut(s) 276
SapI GCTCTTC 1 cut(s) 767
Sau96I GGNCC 5 cut(s) 697, 810, 3879, 4005, 4223
SbfI CCTGCAGG 1 cut(s) 1533
ScaI AGTACT 1 cut(s) 2974
SchI GAGTC 6 cut(s) 101, 273, 283, 377, 722, 3068
ScrFI CCNGG 9 cut(s) 575, 1013, 1710, 1893, 2504, 3632, 3950, 4110, 4137
SdaI CCTGCAGG 1 cut(s) 1533
SduI GDGCHC 8 cut(s) 150, 896, 1706, 2212, 2903, 3456, 3613, 3756
SexAI ACCWGGT 1 cut(s) 3948
SfcI CTRYAG 6 cut(s) 1224, 1529, 2424, 3096, 3226, 3897
SfoI GGCGCC 1 cut(s) 207
SgrAI CRCCGGYG 1 cut(s) 182
SinI GGWCC 1 cut(s) 697
SmiMI CAYNNNNRTG 3 cut(s) 392, 1167, 2533
SmlI CTYRAG 5 cut(s) 1253, 1384, 2101, 2608, 3642
SmoI CTYRAG 5 cut(s) 1253, 1384, 2101, 2608, 3642
SphI GCATGC 2 cut(s) 355, 1885
Sse8387I CCTGCAGG 1 cut(s) 1533
SseBI AGGCCT 1 cut(s) 3421
SspDI GGCGCC 1 cut(s) 205
StuI AGGCCT 1 cut(s) 3421
StyD4I CCNGG 9 cut(s) 573, 1011, 1708, 1891, 2502, 3630, 3948, 4108, 4135
StyI CCWWGG 5 cut(s) 387, 3122, 4001, 4008, 4335
TaiI ACGT 3 cut(s) 452, 807, 1441
TatI WGTACW 3 cut(s) 2972, 3021, 3235
TauI GCSGC 2 cut(s) 339, 726
TscAI CASTG 8 cut(s) 74, 680, 968, 1618, 3054, 3214, 3532, 4366
TseFI GTSAC 2 cut(s) 983, 1015
Tsp45I GTSAC 2 cut(s) 983, 1015
TspGWI ACGGA 4 cut(s) 848, 1065, 4066, 4106
TspRI CASTG 8 cut(s) 74, 680, 968, 1618, 3054, 3214, 3532, 4366
Van91I CCANNNNNTGG 2 cut(s) 1457, 3410
VneI GTGCAC 1 cut(s) 3609
VpaK11BI GGWCC 1 cut(s) 697
XagI CCTNNNNNAGG 2 cut(s) 704, 1532
XapI RAATTY 6 cut(s) 284, 1690, 1975, 2752, 3287, 4030
XceI RCATGY 4 cut(s) 355, 1885, 2659, 3663
XcmI CCANNNNNNNNNTGG 1 cut(s) 3411
XmiI GTMKAC 1 cut(s) 277
XmnI GAANNNNTTC 3 cut(s) 1627, 1902, 2984
ZrmI AGTACT 1 cut(s) 2974
Zsp2I ATGCAT 3 cut(s) 1924, 2449, 3349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.