RchiOBHm_Chr1g0331571

Phosphoinositide 3-kinase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001323
Physical Location & Seq
Forward (+)
21419415 .. 21427774
8360 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56059

Sequence Viewer

Length: 4668 bp
ATGGGGAACAAGATCGCGCGGACGACCCAGGTGTCGGCGTCGGAGTATTACCTGCACGACCTGCCGTCGTCCTACAACCTCGTGCTGAAAGAGGTTTTAGGTCGCGGACGCTTCTTCAAGTCGATTCAGTGCAAGCACGACGAGGGTTTGGTCCTCGTCAAGGTCTATTTCAAGCGCGGCGACTCCATCGATCTCCGCGAGTACGAGCGCCGTCTCTTCCATATCAAGGAGACCTTTCGCGCCCTCGATCACCCTCACCTCTGGCCCTTCCAGTTTTGGCAAGAGACGGATAAAGCAGCTTATCTTGTGAGACAATACTTCTTCAATAATCTGCATGATCGATTGAGCACGCGCCCCTTTCTCAGTCTCATCGAAAAGAAATGGTTGGCTTTTCAGTTACTTCTTGCTGTGAAACAGTGCCATGACAAAGGAATATGTCACGGTGACATTAAGTGCGAGAATGTCCTGGTGACTTCTTGGAATTGGCTCTATCTTGCTGACTTTGCATCCTTCAAACCCACTTACATTCCGTATGATGACCCTTCTGATTTCTCGTTTTTCTACGACACCGGAGGAAGAAGGCTTTGTTATCTTGCTCCCGAGAGATTTTATGAGCATGGAGGTGAGATGCAAGTTGCGCAAGATGCACCATTAAGACCATCTATGGATATCTTTGCTGTAGGGTGTGTGATTGCTGAGCTTTTTCTTGAGGGTCAGCCCCTATTTGAACTCTCTCAACTTCTCGCTTATCGTAGAGGGCAATACGATCCTAGTCAACTTCTTGAAAAGATACCAGATTGTGGAATCCGCAAAATGATACTTCATATGATTCAGTTGGAACCAGAGTTGCGACTTTCTGCTGATAGCTACCTGCAGGAGTATACAACTATTGTGTTTCCGAGTTACTTCTCTCCATTTCTGCATAATTTTCATTGTTTCTGGAATCCGCTTCATTCTGATATGAGGGTTGCACTTTGCCAGAGTGTTTTTCCTGAGATACTGAAACAAATGATGAGCAATAGGTCATCTCAGGACACTAGCACTGGACATGGAACTCCTTCAAACATCCATGCTGTTAGCGGTAAATCTTCTCAGGACATGATTGCAATGCAGAACATGAATTTGACAAAGGGCTCACCGGGAAAGAAAGTAGAGATGGATAAAGGTTTCAAGCGCGATCAATTTGAACTTCTCGGTGATATCAATACGCTTCTTAGGGATGTTAAACAGAGCAATCATTATTCTAATATGAAGCCAATGGTGGAGGACAACACTAATTCTGCATTTTCCCAGAATCTTGGAAACTATGGCATGCAATCTCCTGGTGAATTACTTCAGACTATCTCCCATGCATTTAGGAGAAATGATCATCCTTTTCTAAAAAAGATTACAATGAATGATCTGAATTCATTGATGTCCAAGTATGACAGCCAGTCAGATACCTTTGGTATGCCTTTTTTACCATTACCTGAAGACAGTTTGAGATGTGAGGGCATGGTTCTGATTACCTCTTTGCTATGTTCCTGCATACGCAATGTCAAATTGCCTCATTTAAGGAGGAGGGCGATACTTTTGCTGAAGTCATCTGCATTGTATATTGATGATGAAAACAGGTTGCAGCGTGTAATTCCATATGTTGTTGCAATGCTTTCAGATCAAGCTGCAATTGTGCGTTGTGCAGCCTTGGAGACGTTATGTGACATTCTCCCTTTAGTTCGAGATTTTCCTCCTAGTGATGCAAAAATTTTTCCAGAGTATATTCTACCAATGCTTTCCATGCTTCCTGATGATACAGAGGAAAGTGTGAGGATATGTTATGCCAGTAATATAGCTAAGCTGGCACTAACTGCTTATGGTTTTTTAGTTCACTCAATAACCTTGAGTGAGGCTGGTGTGCTTGATGAATTAAGTTCTCCAAAGAAGCTACTGGCATCATCTAGTGAGACATCTGGTCAGCTCCACAAGGTAAATAATGATGCACAGCTTGCCCAGTTGAGGAAGTCCATAGCTGAAGTCATTCAAGAGCTTGTTATGGGTCCCAAGCAAACTCCCAATATCAGGAGAGCACTCCTTCAGGATATCAGCAATCTATGCTGCTTCTTTGGCCAGAGGCAGAGTAATGACTTTTTGTTACCCATTCTCCCTGCTTTTCTGAATGATCGAGATGAGCAGCTAAGAGCAGTATTCTACGGGCAAATTGTGTATGTATGCTTCTTTGTGGGTCAAAGAAGTGTGGAAGAATATCTTTTACCTTATATTGAGCAGGCTGTAAGTGATGCTACAGAGGCTGTTATTGTCAATGCATTGGACTGTTTGGCCATTTTGTGCAAAAGTGGTTATCTGCGCAAGAGGATACTCCTGGAAATGATTGAGCGTTCATTTCCATTGTTATGCTATCCTAGTCAATGGGTAAGAAGGTCGGCTGTTAGTTTTATTGCAGCCAGCAGTGATTGCTTGGGTGCAGTAGATTCCTATGTATTCCTTGCTCCTGTTATTCGTCCCCTTCTTCGTAGACAGCCAGCATCTCTAGCTTCTGAGAAGGCTCTCCTTTCATGTTTGAAGCCTCCTGTCTCAAGACAGGTATTTTACCAAGTTCTAGAAAATGCAAGGAGTTCAGACATGTTGGAAAGACAGAGAAAGATATGGTACAATTCTTGGCCTCAATCTAAACAATGGGAAAGTGTGGATTTACTTCATAAAGGGGTTGTCGAATTGAATTCGATGAGGAGCTGGACAGACGACCAAGAAAGTCCCGAGGGTCAAAAACATTCTGGAAATGATTTGCAACAGGGAAAGCTAACTGAATGTGATGATGGTGAGGCTAAGTTTGGATGTATGGGAAGCTTTACGCGCAATGGTTCGAGCACAGTTGACATTCATGATCCTTTATCCTCTGAAAAGTTGCAGTATTCGGGCTTTATGTGGCCACAGGGAAGTACTGTGAATAGCTTCATGTGTGATAAATCATCAGTAGGGATACCTTTGTACTCATTTAGCATGGACAGGCGAGCAGTGGGAGTTCCTCCTGCTCCATCTGACTCTTCATTACAAGTGAGTTCTGTAGGAGTTGGTGCATCATCTATGCCATGGATGGATCCAGTAAATAAATCTTTTAGTTTAGCTAGTTCTGTTCCAGCACCCAAGCTCGTGTCAGGATCATTCAACATCAGCAGTGGTTCTAAACAATTCTACAGAGTAGTACATGAACCAGATGGTAGGGATAATGACCAAACAACCTTTGTCAATAGCAAATTTCAAGATATGGGGTTATCTAGTGCAACAAAAGCAAGTTCTATTACTGTGGAAGATGCATCCTCTACAAGTGATCTAACAGGATTGCCCTCTTCTACAAGAGCTTCGTCAATTCCAGATTCTGGTTGGAGGCCTCGTGGGGTTTTGGTTGCACACTTGCAAGAGCACCGGTCTGCTGTGAATGACATAGCCGTATCAACTGATCATAGCTTCTTTGTGAGTGCATCTGATGATTCCACTGTCAAGGTATGGGATTCGAGAAAGTTGGAAAAGGACATCTCCTTTAGGTCAAGATTGACTTATCATATGGAGGGAAGCCGTGCACTATGTTCTGTAATGCTCCGGGGCTGCGCTCAAGTTGTAGTTGGAGCATGTGATGGCATGATACATATGTTTTCTGTTGATTACATTTCCAGAGGTCTCGGTAATGTAGTTGAGAAGTATTCAGGTGTTGCTGATATCAAGAAGAAGGACACTAAAGAAGGGGCAATTCTTAGCCTTTTGAATTTCTCTGCTGACAACTATGCAAATCAGATGGTTATGTACAGTACCCAAAATTGTGGGATCCATCTTTGGGATATAAGAACGAATTCAGATTCTTGGACATTGAAAGCAACTCCGGAGGAGGGCTATGTATCTTCCCTAGTAACAGGCCCTTGTGAAAATTGGTTTGTGTCAGGATCTTCAAGGGGTGTGCTCACTCTTTGGGATATGAGGTTCCTTATTCCTGTGAATACATGGCAGTATTCTGCTGTTTGCCCCATAGAGAAAATGTGTCTTTTCCTTCCTCCTCCAAATGCTTCTGTTTCTGCTGCTGCAAGGCCCCTTGTTTATGTTGCTGCAGGTTGCAATGAAGTCTCTCTTTGGAATGCAGAAAATGGCACCTGCCACCAGGTGTTGAGGGTTGCGAGTTATGAAAGTGATGCTGAAATGTCTGAGGTGCCTTGGGCTTTGGCAAGATCATCAACTAAAAATTCCAAAGCGGATATGAGACGGAATGTCAATCCTCATTACAGAGTTGATGAACTGAATGAACCACCTCCTCGCATTCCTGGCATCCGTTCATTGCTTCCCTTACCTGGGGGTGATTTGTTAACTGGGGGAACCGACTTGAAGATACGTCGATGGGATCATTACAGCCCTGATCGAAGTTACTGTATTTGTGGGCCAAACTTGAAGGGTGTTGGAAATGATGATTTTTATGGAATGCGGTCTAGCTTTGGCGTGCAAGTTGTGCAGGAGACAAAGAGACGGCCTCTAACAACAAAGTTGACAGCCAAGGCAGTTCTTGCAGCTGCTGCCACTGATACTGCTGGCTGCCATCGTGATTCAATCCTTTCATTGGCCTCTGTTAAGTTGAATCAAAGACACTTAATATCAAGCAGTAGAGATGGGGCAATCAAGGTCTGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000011 GO:0000151 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005737 GO:0005768 GO:0005770 GO:0005942 GO:0006355 GO:0006357 GO:0006464 GO:0006468 GO:0006605 GO:0006623 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006886 GO:0006914 GO:0006996 GO:0007033 GO:0007034 GO:0007154 GO:0007165 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009555 GO:0009846 GO:0009856 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010008 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0016236 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019219 GO:0019222 GO:0019538 GO:0019898 GO:0022414 GO:0023052 GO:0030242 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031410 GO:0031461 GO:0031967 GO:0031975 GO:0031982 GO:0032182 GO:0032501 GO:0032502 GO:0032507 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0033036 GO:0033365 GO:0034067 GO:0034243 GO:0034271 GO:0034272 GO:0034613 GO:0035032 GO:0035556 GO:0036211 GO:0042886 GO:0043130 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0044706 GO:0045053 GO:0045184 GO:0045185 GO:0045324 GO:0045893 GO:0045935 GO:0045944 GO:0046907 GO:0048015 GO:0048017 GO:0048229 GO:0048308 GO:0048518 GO:0048522 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051235 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051651 GO:0051704 GO:0051716 GO:0060255 GO:0061695 GO:0061919 GO:0065007 GO:0065008 GO:0070727 GO:0071561 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0080008 GO:0080090 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0120095 GO:0140096 GO:1901564 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04136 ko04138 ko04140 ko04371 map04136 map04138 map04140 map04371
Pfam Domains
Protein Families
HEAT Pkinase WD40

Protein Analysis

1555

Amino Acids

174.38

Weight (kDa)

6.85

Isoelectric Point (pI)

51.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 28 - 240 1.9e-07 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 29 - 292 2.9e-21 Protein kinase domain
VPS15-like_hel PF22956 502 - 865 1.7e-123 VPS15-like, helical domain
Beta-prop_WDR5 PF25175 1131 - 1218 4.7e-08 WDR5 beta-propeller domain
WD40_Prp19 PF24814 1131 - 1337 1.8e-11 Prp19 WD40 domain
WD40 PF00400 1132 - 1169 1.3e-08 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 1135 - 1332 2.7e-10 THOC3 beta-propeller domain
WD40_Gbeta PF25391 1137 - 1344 1.3e-07 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 1141 - 1342 1.7e-07 WDHD1 first WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 4145
AasI GACNNNNNNGTC 1 cut(s) 31
Acc16I TGCGCA 2 cut(s) 639, 2345
Acc36I ACCTGC 5 cut(s) 60, 69, 879, 4085, 4145
AccB1I GGYRCC 2 cut(s) 4133, 4192
AccB7I CCANNNNNTGG 2 cut(s) 800, 3374
AccI GTMKAC 2 cut(s) 881, 2512
AccII CGCG 9 cut(s) 17, 19, 105, 177, 198, 240, 352, 1176, 2851
AccIII TCCGGA 1 cut(s) 3871
AciI CCGC 9 cut(s) 19, 105, 177, 196, 808, 947, 1080, 4235, 4462
AclWI GGATC 9 cut(s) 761, 2876, 3089, 3102, 3163, 3811, 3824, 3940, 4389
AcoI YGGCCR 3 cut(s) 2104, 2316, 2924
AcsI RAATTY 8 cut(s) 1120, 1405, 1743, 2716, 3251, 3757, 3841, 4225
AcuI CTGAAG 5 cut(s) 1319, 1491, 1598, 2031, 2057
AcyI GRCGYC 1 cut(s) 38
AdeI CACNNNGTG 1 cut(s) 4147
AfaI GTAC 7 cut(s) 203, 2648, 2938, 2987, 3201, 3797, 3802
AflIII ACRYGT 1 cut(s) 2619
AgeI ACCGGT 1 cut(s) 3420
AhdI GACNNNNNGTC 4 cut(s) 64, 1432, 1950, 4250
AjnI CCWGG 7 cut(s) 27, 465, 1321, 2358, 4143, 4303, 4330
AloI GAACNNNNNNTCC 2 cut(s) 3953, 3985
Alw21I GWGCWC 6 cut(s) 350, 2068, 2867, 3420, 3577, 3951
Alw44I GTGCAC 1 cut(s) 3573
AlwI GGATC 9 cut(s) 761, 2876, 3089, 3102, 3163, 3811, 3824, 3940, 4389
AlwNI CAGNNNCTG 3 cut(s) 2288, 3374, 4550
Ama87I CYCGRG 2 cut(s) 599, 2753
Aor13HI TCCGGA 1 cut(s) 3871
ApaLI GTGCAC 1 cut(s) 3573
ApoI RAATTY 8 cut(s) 1120, 1405, 1743, 2716, 3251, 3757, 3841, 4225
AsiGI ACCGGT 1 cut(s) 3420
Asp700I GAANNNNTTC 5 cut(s) 1057, 1332, 2016, 2948, 3841
AspS9I GGNCC 6 cut(s) 151, 264, 2036, 3905, 4074, 4418
AsuC2I CCSGG 2 cut(s) 1140, 3596
AvaI CYCGRG 2 cut(s) 599, 2753
AvaII GGWCC 2 cut(s) 151, 2036
BaeGI GKGCMC 1 cut(s) 3577
BaeI ACNNNNGTAYC 4 cut(s) 2969, 3002, 3629, 3662
BalI TGGCCA 3 cut(s) 2106, 2318, 2926
BamHI GGATCC 2 cut(s) 3094, 3816
BanI GGYRCC 2 cut(s) 4133, 4192
BanII GRGCYC 1 cut(s) 1136
BarI GAAGNNNNNNTAC 2 cut(s) 2195, 2227
BauI CACGAG 3 cut(s) 80, 3146, 3387
BbsI GAAGAC 1 cut(s) 1479
Bbv12I GWGCWC 6 cut(s) 350, 2068, 2867, 3420, 3577, 3951
BceAI ACGGC 5 cut(s) 49, 195, 3428, 3555, 4520
BcgI CGANNNNNNTGC 2 cut(s) 1555, 1589
BciT130I CCWGG 7 cut(s) 29, 467, 1323, 2360, 4145, 4305, 4332
BciVI GTATCC 2 cut(s) 2346, 2970
BclI TGATCA 2 cut(s) 1366, 3454
BcnI CCSGG 2 cut(s) 1140, 3596
BfmI CTRYAG 6 cut(s) 678, 872, 2280, 3060, 3190, 4092
BfoI RGCGCY 1 cut(s) 211
BfuAI ACCTGC 5 cut(s) 60, 69, 879, 4085, 4145
BfuI GTATCC 2 cut(s) 2346, 2970
BlpI GCTNAGC 2 cut(s) 696, 1833
BmcAI AGTACT 1 cut(s) 2938
Bme1390I CCNGG 9 cut(s) 29, 467, 1140, 1323, 2360, 3596, 4145, 4305, 4332
Bme18I GGWCC 2 cut(s) 151, 2036
BmeRI GACNNNNNGTC 4 cut(s) 64, 1432, 1950, 4250
BmeT110I CYCGRG 2 cut(s) 599, 2753
BmgT120I GGNCC 6 cut(s) 151, 264, 2036, 3905, 4074, 4418
BmrFI CCNGG 9 cut(s) 29, 467, 1140, 1323, 2360, 3596, 4145, 4305, 4332
BmrI ACTGGG 2 cut(s) 1984, 4359
BmuI ACTGGG 2 cut(s) 1984, 4359
BpiI GAAGAC 1 cut(s) 1479
BplI GAGNNNNNCTC 2 cut(s) 4492, 4524
Bpu1102I GCTNAGC 2 cut(s) 696, 1833
BpuEI CTTGAG 4 cut(s) 728, 1900, 2557, 3591
BpuMI CCSGG 2 cut(s) 1140, 3596
Bsa29I ATCGAT 2 cut(s) 189, 340
BsaHI GRCGYC 1 cut(s) 38
BsaI GGTCTC 2 cut(s) 224, 3677
BsaJI CCNNGG 8 cut(s) 27, 1683, 2754, 3086, 3595, 4196, 4331, 4530
BsaWI WCCGGW 3 cut(s) 569, 3420, 3871
BsaXI ACNNNNNCTCC 4 cut(s) 2124, 2154, 3373, 3403
Bse118I RCCGGY 1 cut(s) 3420
Bse1I ACTGG 8 cut(s) 271, 1048, 1432, 1821, 1932, 1990, 3098, 4354
Bse3DI GCAATG 6 cut(s) 1113, 1540, 1650, 2860, 4108, 4316
BseAI TCCGGA 1 cut(s) 3871
BseBI CCWGG 7 cut(s) 29, 467, 1323, 2360, 4145, 4305, 4332
BseCI ATCGAT 2 cut(s) 189, 340
BseDI CCNNGG 8 cut(s) 27, 1683, 2754, 3086, 3595, 4196, 4331, 4530
BseGI GGATG 8 cut(s) 506, 1065, 1225, 1369, 2837, 3096, 3311, 4308
BseMI GCAATG 6 cut(s) 1113, 1540, 1650, 2860, 4108, 4316
BseMII CTCAG 7 cut(s) 376, 687, 984, 1043, 1106, 2526, 4179
BseNI ACTGG 8 cut(s) 271, 1048, 1432, 1821, 1932, 1990, 3098, 4354
BseRI GAGGAG 5 cut(s) 1573, 2740, 3890, 4032, 4284
BseSI GKGCMC 1 cut(s) 3577
BsgI GTGCAG 4 cut(s) 38, 1698, 2481, 4508
Bsh1236I CGCG 9 cut(s) 17, 19, 105, 177, 198, 240, 352, 1176, 2851
BshNI GGYRCC 2 cut(s) 4133, 4192
BshTI ACCGGT 1 cut(s) 3420
BshVI ATCGAT 2 cut(s) 189, 340
BsiHKAI GWGCWC 6 cut(s) 350, 2068, 2867, 3420, 3577, 3951
BsiHKCI CYCGRG 2 cut(s) 599, 2753
BsiSI CCGG 5 cut(s) 570, 1139, 3421, 3595, 3872
BslFI GGGAC 3 cut(s) 2022, 2484, 2736
BsmBI CGTCTC 5 cut(s) 218, 278, 1682, 4237, 4495
BsmFI GGGAC 3 cut(s) 2022, 2484, 2736
BsmI GAATGC 3 cut(s) 4126, 4299, 4464
Bso31I GGTCTC 2 cut(s) 224, 3677
BsoBI CYCGRG 2 cut(s) 599, 2753
Bsp1286I GDGCHC 7 cut(s) 350, 1136, 2068, 2867, 3420, 3577, 3951
Bsp13I TCCGGA 1 cut(s) 3871
Bsp1407I TGTACA 1 cut(s) 3795
Bsp1720I GCTNAGC 2 cut(s) 696, 1833
Bsp19I CCATGG 1 cut(s) 3086
BspACI CCGC 9 cut(s) 19, 105, 177, 196, 808, 947, 1080, 4235, 4462
BspCNI CTCAG 7 cut(s) 375, 688, 985, 1042, 1105, 2527, 4180
BspDI ATCGAT 2 cut(s) 189, 340
BspEI TCCGGA 1 cut(s) 3871
BspFNI CGCG 9 cut(s) 17, 19, 105, 177, 198, 240, 352, 1176, 2851
BspHI TCATGA 1 cut(s) 2878
BspMAI CTGCAG 2 cut(s) 876, 4096
BspMI ACCTGC 5 cut(s) 60, 69, 879, 4085, 4145
BspPI GGATC 9 cut(s) 761, 2876, 3089, 3102, 3163, 3811, 3824, 3940, 4389
BspT107I GGYRCC 2 cut(s) 4133, 4192
BspTNI GGTCTC 2 cut(s) 224, 3677
BsrDI GCAATG 6 cut(s) 1113, 1540, 1650, 2860, 4108, 4316
BsrFI RCCGGY 1 cut(s) 3420
BsrGI TGTACA 1 cut(s) 3795
BsrI ACTGG 8 cut(s) 271, 1048, 1432, 1821, 1932, 1990, 3098, 4354
BssAI RCCGGY 1 cut(s) 3420
BssECI CCNNGG 8 cut(s) 27, 1683, 2754, 3086, 3595, 4196, 4331, 4530
BssNAI GTATAC 1 cut(s) 882
BssNI GRCGYC 1 cut(s) 38
BssSI CACGAG 3 cut(s) 80, 3146, 3387
BssT1I CCWWGG 4 cut(s) 1683, 3086, 4196, 4530
Bst1107I GTATAC 1 cut(s) 882
Bst2BI CACGAG 3 cut(s) 80, 3146, 3387
Bst2UI CCWGG 7 cut(s) 29, 467, 1323, 2360, 4145, 4305, 4332
Bst6I CTCTTC 3 cut(s) 221, 3046, 3349
BstACI GRCGYC 1 cut(s) 38
BstAPI GCANNNNNTGC 8 cut(s) 61, 1847, 1985, 2091, 2451, 4486, 4541, 4550
BstAUI TGTACA 1 cut(s) 3795
BstDSI CCRYGG 1 cut(s) 3086
BstENI CCTNNNNNAGG 1 cut(s) 158
BstF5I GGATG 8 cut(s) 506, 1065, 1225, 1369, 2837, 3096, 3311, 4308
BstFNI CGCG 9 cut(s) 17, 19, 105, 177, 198, 240, 352, 1176, 2851
BstH2I RGCGCY 1 cut(s) 211
BstNI CCWGG 7 cut(s) 29, 467, 1323, 2360, 4145, 4305, 4332
BstNSI RCATGY 3 cut(s) 1315, 2623, 3627
BstSCI CCNGG 9 cut(s) 27, 465, 1138, 1321, 2358, 3594, 4143, 4303, 4330
BstSFI CTRYAG 6 cut(s) 678, 872, 2280, 3060, 3190, 4092
BstSLI GKGCMC 1 cut(s) 3577
BstUI CGCG 9 cut(s) 17, 19, 105, 177, 198, 240, 352, 1176, 2851
BstV2I GAAGAC 1 cut(s) 1479
BstX2I RGATCY 3 cut(s) 3094, 3816, 3932
BstXI CCANNNNNNTGG 2 cut(s) 1298, 3812
BstYI RGATCY 3 cut(s) 3094, 3816, 3932
BstZ17I GTATAC 1 cut(s) 882
Bsu15I ATCGAT 2 cut(s) 189, 340
BsuI GTATCC 2 cut(s) 2346, 2970
BsuTUI ATCGAT 2 cut(s) 189, 340
BtgI CCRYGG 1 cut(s) 3086
BtsCI GGATG 8 cut(s) 506, 1065, 1225, 1369, 2837, 3096, 3311, 4308
BtsI GCAGTG 3 cut(s) 2452, 3018, 3178
BtsIMutI CAGTG 8 cut(s) 134, 422, 1041, 2452, 3018, 3178, 3489, 4554
BveI ACCTGC 5 cut(s) 60, 69, 879, 4085, 4145
CaiI CAGNNNCTG 3 cut(s) 2288, 3374, 4550
CciI TCATGA 1 cut(s) 2878
Cfr10I RCCGGY 1 cut(s) 3420
Cfr13I GGNCC 6 cut(s) 151, 264, 2036, 3905, 4074, 4418
ClaI ATCGAT 2 cut(s) 189, 340
CseI GACGC 2 cut(s) 27, 117
CsiI ACCWGGT 1 cut(s) 4143
Csp6I GTAC 7 cut(s) 202, 2647, 2937, 2986, 3200, 3796, 3801
CspAI ACCGGT 1 cut(s) 3420
CviQI GTAC 7 cut(s) 202, 2647, 2937, 2986, 3200, 3796, 3801
DraIII CACNNNGTG 1 cut(s) 4147
DrdI GACNNNNNNGTC 1 cut(s) 31
DriI GACNNNNNGTC 4 cut(s) 64, 1432, 1950, 4250
DseDI GACNNNNNNGTC 1 cut(s) 31
EaeI YGGCCR 3 cut(s) 2104, 2316, 2924
Eam1104I CTCTTC 3 cut(s) 221, 3046, 3349
Eam1105I GACNNNNNGTC 4 cut(s) 64, 1432, 1950, 4250
EarI CTCTTC 3 cut(s) 221, 3046, 3349
Eco130I CCWWGG 4 cut(s) 1683, 3086, 4196, 4530
Eco147I AGGCCT 1 cut(s) 3385
Eco24I GRGCYC 1 cut(s) 1136
Eco31I GGTCTC 2 cut(s) 224, 3677
Eco32I GATATC 4 cut(s) 670, 1201, 2080, 3712
Eco47I GGWCC 2 cut(s) 151, 2036
Eco57I CTGAAG 5 cut(s) 1319, 1491, 1598, 2031, 2057
Eco88I CYCGRG 2 cut(s) 599, 2753
EcoNI CCTNNNNNAGG 1 cut(s) 158
EcoO109I RGGNCCY 3 cut(s) 2036, 3905, 4074
EcoRI GAATTC 3 cut(s) 1405, 2716, 3841
EcoRII CCWGG 7 cut(s) 27, 465, 1321, 2358, 4143, 4303, 4330
EcoRV GATATC 4 cut(s) 670, 1201, 2080, 3712
EcoT14I CCWWGG 4 cut(s) 1683, 3086, 4196, 4530
EcoT22I ATGCAT 3 cut(s) 1354, 2305, 3313
EcoT38I GRGCYC 1 cut(s) 1136
ErhI CCWWGG 4 cut(s) 1683, 3086, 4196, 4530
Esp3I CGTCTC 5 cut(s) 218, 278, 1682, 4237, 4495
FalI AAGNNNNNCTT 8 cut(s) 218, 250, 2229, 2261, 3535, 3567, 4098, 4130
FaqI GGGAC 3 cut(s) 2022, 2484, 2736
FauNDI CATATG 4 cut(s) 825, 1633, 3558, 3642
FbaI TGATCA 2 cut(s) 1366, 3454
FblI GTMKAC 2 cut(s) 881, 2512
FokI GGATG 8 cut(s) 493, 1052, 1232, 1356, 2844, 3103, 3298, 4295
FriOI GRGCYC 1 cut(s) 1136
FspI TGCGCA 2 cut(s) 639, 2345
HaeII RGCGCY 1 cut(s) 211
HapII CCGG 5 cut(s) 570, 1139, 3421, 3595, 3872
HgaI GACGC 2 cut(s) 27, 117
Hin1I GRCGYC 1 cut(s) 38
HincII GTYRAC 4 cut(s) 776, 2872, 4347, 4524
HindII GTYRAC 4 cut(s) 776, 2872, 4347, 4524
HindIII AAGCTT 1 cut(s) 2842
HpaI GTTAAC 1 cut(s) 4347
HpaII CCGG 5 cut(s) 570, 1139, 3421, 3595, 3872
Hpy166II GTNNAC 8 cut(s) 776, 882, 1867, 2513, 2872, 3575, 4347, 4524
Hpy8I GTNNAC 8 cut(s) 776, 882, 1867, 2513, 2872, 3575, 4347, 4524
Hpy99I CGWCG 4 cut(s) 43, 70, 143, 4377
HpyCH4IV ACGT 2 cut(s) 1691, 4372
HpySE526I ACGT 2 cut(s) 1691, 4372
Hsp92I GRCGYC 1 cut(s) 38
KflI GGGWCCC 1 cut(s) 2036
Kpn2I TCCGGA 1 cut(s) 3871
Ksp22I TGATCA 2 cut(s) 1366, 3454
KspAI GTTAAC 1 cut(s) 4347
LmnI GCTCC 7 cut(s) 601, 1962, 2491, 2727, 3034, 3597, 3620
MabI ACCWGGT 1 cut(s) 4143
MaeII ACGT 2 cut(s) 1691, 4372
MaeIII GTNAC 9 cut(s) 396, 437, 443, 469, 902, 1697, 2130, 3898, 4403
MfeI CAATTG 1 cut(s) 1665
MflI RGATCY 3 cut(s) 3094, 3816, 3932
MhlI GDGCHC 7 cut(s) 350, 1136, 2068, 2867, 3420, 3577, 3951
MlsI TGGCCA 3 cut(s) 2106, 2318, 2926
MluNI TGGCCA 3 cut(s) 2106, 2318, 2926
MlyI GAGTC 2 cut(s) 176, 3032
MmeI TCCRAC 7 cut(s) 21, 816, 2604, 3359, 3498, 3598, 4417
Mox20I TGGCCA 3 cut(s) 2106, 2318, 2926
Mph1103I ATGCAT 3 cut(s) 1354, 2305, 3313
MroI TCCGGA 1 cut(s) 3871
MroXI GAANNNNTTC 5 cut(s) 1057, 1332, 2016, 2948, 3841
MscI TGGCCA 3 cut(s) 2106, 2318, 2926
MseI TTAA 8 cut(s) 450, 653, 1224, 1553, 1907, 4346, 4605, 4625
MslI CAYNNNNRTG 2 cut(s) 621, 2389
Msp20I TGGCCA 3 cut(s) 2106, 2318, 2926
MspA1I CMGCKG 1 cut(s) 4547
MspI CCGG 5 cut(s) 570, 1139, 3421, 3595, 3872
MspR9I CCNGG 9 cut(s) 29, 467, 1140, 1323, 2360, 3596, 4145, 4305, 4332
MunI CAATTG 1 cut(s) 1665
Mva1269I GAATGC 3 cut(s) 4126, 4299, 4464
MvaI CCWGG 7 cut(s) 29, 467, 1323, 2360, 4145, 4305, 4332
MvnI CGCG 9 cut(s) 17, 19, 105, 177, 198, 240, 352, 1176, 2851
NciI CCSGG 2 cut(s) 1140, 3596
NcoI CCATGG 1 cut(s) 3086
NdeI CATATG 4 cut(s) 825, 1633, 3558, 3642
NmuCI GTSAC 4 cut(s) 437, 443, 469, 1697
NsbI TGCGCA 2 cut(s) 639, 2345
NsiI ATGCAT 3 cut(s) 1354, 2305, 3313
NspI RCATGY 3 cut(s) 1315, 2623, 3627
PaeI GCATGC 1 cut(s) 1315
PagI TCATGA 1 cut(s) 2878
PaqCI CACCTGC 1 cut(s) 4145
PceI AGGCCT 1 cut(s) 3385
PciI ACATGT 1 cut(s) 2619
PcsI WCGNNNNNNNCGW 1 cut(s) 195
PctI GAATGC 3 cut(s) 4126, 4299, 4464
PdmI GAANNNNTTC 5 cut(s) 1057, 1332, 2016, 2948, 3841
PflMI CCANNNNNTGG 2 cut(s) 800, 3374
PfoI TCCNGGA 1 cut(s) 2358
PinAI ACCGGT 1 cut(s) 3420
PleI GAGTC 2 cut(s) 176, 3032
PpsI GAGTC 2 cut(s) 176, 3032
PpuMI RGGWCCY 1 cut(s) 2036
PscI ACATGT 1 cut(s) 2619
Psp5II RGGWCCY 1 cut(s) 2036
Psp6I CCWGG 7 cut(s) 27, 465, 1321, 2358, 4143, 4303, 4330
PspGI CCWGG 7 cut(s) 27, 465, 1321, 2358, 4143, 4303, 4330
PspPI GGNCC 6 cut(s) 151, 264, 2036, 3905, 4074, 4418
PspPPI RGGWCCY 1 cut(s) 2036
PstI CTGCAG 2 cut(s) 876, 4096
PstNI CAGNNNCTG 3 cut(s) 2288, 3374, 4550
PsuI RGATCY 3 cut(s) 3094, 3816, 3932
PvuII CAGCTG 1 cut(s) 4547
RsaI GTAC 7 cut(s) 203, 2648, 2938, 2987, 3201, 3797, 3802
RsaNI GTAC 7 cut(s) 202, 2647, 2937, 2986, 3200, 3796, 3801
RseI CAYNNNNRTG 2 cut(s) 621, 2389
SaqAI TTAA 8 cut(s) 450, 653, 1224, 1553, 1907, 4346, 4605, 4625
Sau96I GGNCC 6 cut(s) 151, 264, 2036, 3905, 4074, 4418
SbfI CCTGCAGG 1 cut(s) 876
ScaI AGTACT 1 cut(s) 2938
SchI GAGTC 2 cut(s) 176, 3032
ScrFI CCNGG 9 cut(s) 29, 467, 1140, 1323, 2360, 3596, 4145, 4305, 4332
SdaI CCTGCAGG 1 cut(s) 876
SduI GDGCHC 7 cut(s) 350, 1136, 2068, 2867, 3420, 3577, 3951
SexAI ACCWGGT 1 cut(s) 4143
SfcI CTRYAG 6 cut(s) 678, 872, 2280, 3060, 3190, 4092
SinI GGWCC 2 cut(s) 151, 2036
SmiMI CAYNNNNRTG 2 cut(s) 621, 2389
SmlI CTYRAG 4 cut(s) 707, 1879, 2572, 3606
SmoI CTYRAG 4 cut(s) 707, 1879, 2572, 3606
SphI GCATGC 1 cut(s) 1315
Sse8387I CCTGCAGG 1 cut(s) 876
SseBI AGGCCT 1 cut(s) 3385
SsiI CCGC 9 cut(s) 19, 105, 177, 196, 808, 947, 1080, 4235, 4462
StuI AGGCCT 1 cut(s) 3385
StyD4I CCNGG 9 cut(s) 27, 465, 1138, 1321, 2358, 3594, 4143, 4303, 4330
StyI CCWWGG 4 cut(s) 1683, 3086, 4196, 4530
TaiI ACGT 2 cut(s) 1694, 4375
TatI WGTACW 4 cut(s) 2936, 2985, 3199, 3795
TauI GCSGC 1 cut(s) 180
Tru1I TTAA 8 cut(s) 450, 653, 1224, 1553, 1907, 4346, 4605, 4625
Tru9I TTAA 8 cut(s) 450, 653, 1224, 1553, 1907, 4346, 4605, 4625
TscAI CASTG 8 cut(s) 134, 422, 1048, 2452, 3018, 3178, 3496, 4561
TseFI GTSAC 4 cut(s) 437, 443, 469, 1697
Tsp45I GTSAC 4 cut(s) 437, 443, 469, 1697
TspGWI ACGGA 4 cut(s) 302, 519, 4261, 4301
TspRI CASTG 8 cut(s) 134, 422, 1048, 2452, 3018, 3178, 3496, 4561
Van91I CCANNNNNTGG 2 cut(s) 800, 3374
VneI GTGCAC 1 cut(s) 3573
VpaK11BI GGWCC 2 cut(s) 151, 2036
XagI CCTNNNNNAGG 1 cut(s) 158
XapI RAATTY 8 cut(s) 1120, 1405, 1743, 2716, 3251, 3757, 3841, 4225
XbaI TCTAGA 1 cut(s) 2596
XceI RCATGY 3 cut(s) 1315, 2623, 3627
XcmI CCANNNNNNNNNTGG 1 cut(s) 3375
XmiI GTMKAC 2 cut(s) 881, 2512
XmnI GAANNNNTTC 5 cut(s) 1057, 1332, 2016, 2948, 3841
ZrmI AGTACT 1 cut(s) 2938
Zsp2I ATGCAT 3 cut(s) 1354, 2305, 3313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.