MD02G1262100.v1.1

Phosphoinositide 3-kinase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001323
Physical Location & Seq
Reverse (-)
31635906 .. 31643956
8051 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1262100.v1.1.491

Sequence Viewer

Length: 4635 bp
ATGGGGAACAAGATCGCTCGAACGACGCAAGTGTCGGCGTCGGAGTACTACCTCCACGACCTGCCGTCGTCCTACAACCTGGTCCTGAAGGAGGTTCTAGGGCGGAGCCGCTTCTTCAAGTCGATTGAGTGCAAGCACGACGAGGGTTTGGTCCTCGTCAAGGTCTATTTCAAGCGCGGTGACTCCATTGATCTCCGCGAGTACGAGCGCCGCCTCTCCATCATCAAGGAAACCTTCCGTACTCTCGATCACCCTCACGTTTGGCCCTTCCAGTTTTGGCAAGAGACAGATAAAGCTGCGTATCTTGTGAGGCAATTCTTCTTCAACAATCTGCACGACCGGTTAAGCACCCGCCCGTTTCTCAGTCTAATCGAGAAGAAATGGTTGGCCTTTCAGTTACTTCTTGCTCTGAAGCAGTGCCATGATAAAGGAATATGTCACGGTGATATTAAGTGTGAGAATGTGTTGGTGACTTCTTGGAATTGGCTCTACCTTGCTGACTTTGCATCCTTCAAACCCACCTACATACCATATGACGATCCTTCTGATTTCTCTTTTTTCTTTGACACGGGAGGAAGAAGGCTTTGTTATCTTGCTCCTGAGAGATTTTATGAGCATGGAGGTGAGATGCAGGTTGCACAAGATGCACCATTAAGGCCATCTATGGACATATTTGCTGTAGGGTGTGTAATTGCTGAGCTTTTCCTTGAGGGACAGCCACTGTTCGAACTCTCTCAACTTCTTGCTTATCGTAGAGGGCAATACGATCCTAGCCAACTTCTTGAAAAGATACCAGATTCTGGGATTCGCAAGATGATACTTCATATGATTCAGTTGGAACCAGAGTTGCGACATTCTGCTGATAGCTACCTTCAGGAGTATACAACCATTGTTTTTCCAAGTTACTTCTCTCCATTTCTGCATAATTTCCATTGTTTCTGGAATCCCCTTCATTCAGATATGAGGGTTGCACTTTGCCAGAGTGTTTTTCATGAGATACTTAAACAAATGATGAGCAGTAGGTCAACTGAGGACACTGGAACAGGACCGGGAACTCCTTCAAGTGCCCATGGCATGAGTGGTAGAACTTCTCAGGAAGCAGTTGTAATGCAGAACAAGGGTTTGACAAGGGGCTCATTTACAAAGAGTGAAGAGATGGGTAAAGGCTTAAAATGTGATCAATTGGAACTTCTCGGTGATATCAATACCCTTCTTCGGGATGTTAAACAAAGTAATAATTATTCTGCTACCAAGCCAATGGTTAACGATAATCCCGATTCTGCATTTTCCCCGAATCTTGGCAACTATGGCATGCAATCTCCTGGTGAATTACTTCAATCTATCTCTAATGCATTTAGGAGAAACGATCATCCTTTCCTGAAAAAGATTACATTGAATGATTTGAATTCTTTGATGTCGAAGTATGACAGCCATTCAGATACCTTTGGCACACCATTTCTGCCATTACCTGAAAACAGTATCAGATGTGAGGGCATGGTTCTGATTACCTCGTTGCTCTGTTCATGCATACGTAATGTAAAATTGCCTCATTTAAGGAGGAGGGCCATACTTTTGCTGAAATCCTCTGCCTTGTATATTGATGATGAAGATCGTTTGCAACGTGTAATTCCATATGTTGTTGCAATGCTTTCAGATGCAGCTGCAATTGTGCGTTGTGCTGCCTTGGAGACGTTGTGTGACATACTACCTCTAGTCCGAGATTTTCCTCCCAGTGATGCAAAAATTTTTCCGGAGTATATTCTGCCAATGCTTTCCATGCTTCCGGATGATCCAGAAGAAAGTGTGAGGATATGTTATGCCAGTAATATTGCTAAGCTGGCGCTAACTGCTTATGGTTTCTTGGTTCACTCAATGCGCTTGAGTGAGGCTGGGGTACTTGATGAATTAAGTTCTGCAAAAAAGCCGCTGGCATCATCTAGTGAGACATCTGGTCAACTTGCTGTGTTGAGGAAGTCTATAGCTGAGGTCATTCAAGAACTTGTTATGGGTCCAAAGCAAACTCCAAATATCAGGAGAGCACTCCTTCAGGACATTAGCAACCTATGCTGCTTCTTTGGCCAGAGACAGAGTAATGACTTTTTGTTACCCATTCTCCCTGCTTTTCTGAATGATCGAGATGAGCAGCTGAGAGCAGTATTCTATGGGCAAATTGTATATGTGTGCTTCTTTGTGGGTCAAAGAAGTGTGGAAGAATATCTATTACCTTACATTGAGCAGGCTGTAAGTGATGTAACAGAGGCTGTAATTGTCAATGCATTGGACTGTTTGGCCATTTTGTGCAAAAGTAGTTTCTTGCGGAAGAGGATACTTCTTGAAATGATTGAGCATGCATTTCCATTGTTATGCTATCCTAGTCAATGGGTAAGAAGGTCAGCTGTCACTTTCATTGCAGCCAGTAGTGACAGCTTAGGTGCTGTAGATTCCTATGTCTTTCTAGCCCCTGTTATTCGCCCCCTACTCCGCAGACAGCCAGCATCTCTTGCTTCTGAAAAGGCTCTCCTTTTGTGTTTAAAGCCTCCAGTCTCAAGACAGGTTTTTTACCAAGTTCTAGAAAATGCAAGGAGTTCAGACATGTTGGAAAGACAGAGAAAGATATGGTACAACTCTTGGCCTCAGTCTAAACAATGGGAAAGTGTTGACTTGCTTCATAAAGGAGTTGAGGAACTGAGTTCAACAGGGAGCTGGCCAGACAAGCAACAAAGTGCCGAAAATCATAAACTTACTGGAAAGGCCTTGCAACAAGGAGAGTTAACTGAATGTGATGATGGTGAGGCTAAGTTGAGAAGTATGGGAAGCTTTACACGGTCTTCTAGCACAGTTGACATTCATGATCCTTTGTCCTCTGAAAAGTTGCAATTTTCAGGGTTTATGTGGCCACAGGGAAGTTATGTGAATAGCTTCATGTGCGATAAATCATCTGTTGGCATACCTTTATACTCCTTTAGCATGGACAAGCGAGCAGTCGGAGGTCCTCCTGCAACATCTGATTCTTCTTCACAAGTGAATTCTGTAGGAATTGGTGCATCAGCTATGCCTTGGATGGATCCAGTAAATAAATCATTTAGTTTGGCTAGTTCTGTTCCAGCACCTAAGTTTGTGTCAGGATCATTCAACATTGGCAATGGTTCCAAACAGTTCTATAGAGTAGTACATGAGCCAGATGGTAGGGATAATGATCAAACAGCCTTTGGTAATAGCAAGCTTCAAGATATGGGATTATCTGGCACTACAAAATGTGCTATTCCTGCGGAAGATGCATCCACAGCTAGTGATCTAACAGGAATGCCGTCTCCTTCACGAAGTTCATCAATTCCAGACTCTGGTTGGAGGCCTCGTGGGGTGTTGGTTGCTCACCTGCAAGAACATCGGTCTGCTGTCAATGACATAGCCATTTCAACTGATCATAGCTTTTTTGTGAGTGCATCTGATGATTCCACTGTTAAGGTGTGGGATTCAAGAAAGTTGGAAAAGGACATATCATTTCGGTCAAGGTTGACTTATCATCTAGAAGGAAGCCGTGCACTGTGCAGTGCAATGCTCCGGGGGTCTGCCCAAGTCGTGGTAGGAGCATGTGATGGCATGATACATATGTTTTCTGTTGATTACATCTCCAGGGGACTTGGTAATGTTGTTGAAAAGTATTCAGGTGTTGCTGATATCAAGAAGAAGGATATCAAGGAAGGTGCCATATTGAGCCTGTTGAATTTCTCAGCGGATAACGGCACAAATCAGATGGTTATGTACAGCACCCAAAATTGTGGGATCCATCTTTGGGATGTAAGGCTGAATACCATTTCTTGGACATTGAGAGCAACTCCTGAGGAGGGCTATGTGTCTTCGCTGGTAACAGGCCCCTGTGAAAATTGGTTTGTGTCAGGATCTTCGAGGGGTGTGCTTACTCTTTGGGATATGAGGTTCCTTATTCCTGTAAACTCATGGCAGTATTCTTCTGTTTGCCCTATAGAGAAAATGTGCCTGTTTCTTCCTCCACCAAATGCCTCTGTGTCTGCTGCTGCAAGGCCCCTTATTTATGTTGCGGCAGGTTCCAACGAAGTTTCTCTTTGGAATGCTGAAAATGGAAGCTGTCACCAGGTGCTGAGAGTGGCAAATTACGAAAGTGATGCTGAAATATCTGAAGTTCCTTGGGCCTTGGCTAAGTCATCAAGTAAAAGCTCCAAACCAGATCTGAGACGGAATGTCAATCCCCACTACAGAGTTGATGAACTAAACGAACCCCCTCCTCGTCTTCCTGGCATCCGTTCATTGCTGCCCTTACCTGGAGGTGATTTGTTAACTGGAGGAACCGACTTGAAGATACGTCGATGGGATCATTACAGTCCCGATCGAAGTTACTCTATTTGTGGACCAAACTTGAAGGGAGTCGGAAATGATGATTTCTATGCAACAAGATCTAGCTTTGGCGTGCAAGTTGTGCAGGAGACGAAAAGACGACCTCTAACATCGAAGCTGACAGCCAAGGCAGTTCTTGCGGCTGCTGCCACTGATTCTGCTGGCTGCCACCGTGATTCAATCCTAGCGTTGGCCTCGGTTAAGCTAAATCAGAGACACTTAATATCAAGCGGTAGAGATGGGGCAATCAAGGTTTGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000011 GO:0000151 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005737 GO:0005768 GO:0005770 GO:0005942 GO:0006355 GO:0006357 GO:0006464 GO:0006468 GO:0006605 GO:0006623 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006886 GO:0006914 GO:0006996 GO:0007033 GO:0007034 GO:0007154 GO:0007165 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009555 GO:0009846 GO:0009856 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010008 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0016236 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019219 GO:0019222 GO:0019538 GO:0019898 GO:0022414 GO:0023052 GO:0030242 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031410 GO:0031461 GO:0031967 GO:0031975 GO:0031982 GO:0032182 GO:0032501 GO:0032502 GO:0032507 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0033036 GO:0033365 GO:0034067 GO:0034243 GO:0034271 GO:0034272 GO:0034613 GO:0035032 GO:0035556 GO:0036211 GO:0042886 GO:0043130 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0044706 GO:0045053 GO:0045184 GO:0045185 GO:0045324 GO:0045893 GO:0045935 GO:0045944 GO:0046907 GO:0048015 GO:0048017 GO:0048229 GO:0048308 GO:0048518 GO:0048522 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051235 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051651 GO:0051704 GO:0051716 GO:0060255 GO:0061695 GO:0061919 GO:0065007 GO:0065008 GO:0070727 GO:0071561 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0080008 GO:0080090 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0120095 GO:0140096 GO:1901564 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04136 ko04138 ko04140 ko04371 map04136 map04138 map04140 map04371
Pfam Domains
Protein Families
HEAT Pkinase WD40

Protein Analysis

1545

Amino Acids

172.27

Weight (kDa)

6.84

Isoelectric Point (pI)

51.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 30 - 292 2.5e-19 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 31 - 240 4.3e-06 Protein tyrosine and serine/threonine kinase
VPS15-like_hel PF22956 502 - 856 1.1e-122 VPS15-like, helical domain
Beta-prop_WDR5 PF25175 1120 - 1207 1.1e-08 WDR5 beta-propeller domain
WD40_Prp19 PF24814 1120 - 1325 3.5e-09 Prp19 WD40 domain
WD40 PF00400 1121 - 1158 9.9e-09 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 1124 - 1319 6.6e-09 THOC3 beta-propeller domain
WD40_Gbeta PF25391 1126 - 1314 4.1e-06 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 1131 - 1332 6.6e-07 WDHD1 first WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3384
AasI GACNNNNNNGTC 1 cut(s) 31
Acc36I ACCTGC 4 cut(s) 69, 622, 3384, 4052
AccB1I GGYRCC 1 cut(s) 3704
AccB7I CCANNNNNTGG 4 cut(s) 800, 3341, 3580, 3819
AccI GTMKAC 1 cut(s) 881
AccII CGCG 2 cut(s) 177, 198
AccIII TCCGGA 2 cut(s) 1750, 1783
AcoI YGGCCR 4 cut(s) 2077, 2289, 2705, 2894
AcsI RAATTY 4 cut(s) 1405, 1743, 3025, 3724
AcuI CTGAAG 5 cut(s) 107, 431, 857, 2030, 4176
AcyI GRCGYC 1 cut(s) 38
AdeI CACNNNGTG 1 cut(s) 4114
AfaI GTAC 7 cut(s) 47, 203, 241, 1896, 2621, 3171, 3764
AflIII ACRYGT 2 cut(s) 1621, 2592
AgeI ACCGGT 1 cut(s) 339
AhdI GACNNNNNGTC 3 cut(s) 64, 1950, 4217
AjnI CCWGG 6 cut(s) 78, 1321, 3632, 4110, 4270, 4297
AjuI GAANNNNNNNTTGG 2 cut(s) 368, 400
AloI GAACNNNNNNTCC 2 cut(s) 3920, 3952
Alw21I GWGCWC 2 cut(s) 2041, 3544
Alw26I GTCTC 9 cut(s) 278, 1682, 1937, 2077, 2548, 3315, 4204, 4454, 4579
Alw44I GTGCAC 1 cut(s) 3540
AlwNI CAGNNNCTG 5 cut(s) 721, 800, 2261, 3341, 4117
Aor13HI TCCGGA 2 cut(s) 1750, 1783
ApaLI GTGCAC 1 cut(s) 3540
ApoI RAATTY 4 cut(s) 1405, 1743, 3025, 3724
AsiGI ACCGGT 1 cut(s) 339
Asp700I GAANNNNTTC 3 cut(s) 1057, 1332, 2918
AspLEI GCGC 4 cut(s) 177, 210, 1843, 1878
AsuC2I CCSGG 2 cut(s) 1050, 3563
AsuII TTCGAA 1 cut(s) 726
AvaII GGWCC 6 cut(s) 82, 151, 1046, 2009, 2990, 4385
AxyI CCTNAGG 1 cut(s) 3840
BaeGI GKGCMC 2 cut(s) 1069, 3544
BaeI ACNNNNGTAYC 2 cut(s) 3596, 3629
BalI TGGCCA 4 cut(s) 2079, 2291, 2707, 2896
BamHI GGATCC 2 cut(s) 3064, 3783
BanI GGYRCC 1 cut(s) 3704
BanII GRGCYC 1 cut(s) 1136
BauI CACGAG 1 cut(s) 3354
BbsI GAAGAC 3 cut(s) 2820, 3849, 4259
Bbv12I GWGCWC 2 cut(s) 2041, 3544
BbvCI CCTCAGC 1 cut(s) 1983
BceAI ACGGC 4 cut(s) 49, 3292, 3522, 3757
BcgI CGANNNNNNTGC 6 cut(s) 3368, 3402, 3895, 3929, 4124, 4158
BciT130I CCWGG 6 cut(s) 80, 1323, 3634, 4112, 4272, 4299
BciVI GTATCC 1 cut(s) 2319
BclI TGATCA 3 cut(s) 1177, 3196, 3421
BcnI CCSGG 2 cut(s) 1050, 3563
BcoDI GTCTC 9 cut(s) 278, 1682, 1937, 2077, 2548, 3315, 4204, 4454, 4579
BfmI CTRYAG 7 cut(s) 678, 1977, 2436, 3030, 3160, 3981, 4231
BfoI RGCGCY 2 cut(s) 211, 1844
BfuAI ACCTGC 4 cut(s) 69, 622, 3384, 4052
BfuI GTATCC 1 cut(s) 2319
BglII AGATCT 2 cut(s) 4204, 4430
BlpI GCTNAGC 2 cut(s) 696, 1833
BmcAI AGTACT 1 cut(s) 47
Bme1390I CCNGG 8 cut(s) 80, 1050, 1323, 3563, 3634, 4112, 4272, 4299
Bme18I GGWCC 6 cut(s) 82, 151, 1046, 2009, 2990, 4385
BmeRI GACNNNNNGTC 3 cut(s) 64, 1950, 4217
BmrFI CCNGG 8 cut(s) 80, 1050, 1323, 3563, 3634, 4112, 4272, 4299
BmrI ACTGGG 1 cut(s) 1725
BmuI ACTGGG 1 cut(s) 1725
BpiI GAAGAC 3 cut(s) 2820, 3849, 4259
BplI GAGNNNNNCTC 2 cut(s) 3820, 3852
BpmI CTGGAG 4 cut(s) 2523, 3616, 4320, 4338
Bpu10I CCTNAGC 2 cut(s) 1983, 2428
Bpu1102I GCTNAGC 2 cut(s) 696, 1833
Bpu14I TTCGAA 1 cut(s) 726
BpuEI CTTGAG 3 cut(s) 728, 1900, 2530
BpuMI CCSGG 2 cut(s) 1050, 3563
BsaAI YACGTR 1 cut(s) 1532
BsaBI GATNNNNATC 2 cut(s) 1608, 3195
BsaHI GRCGYC 1 cut(s) 38
BsaJI CCNNGG 9 cut(s) 1069, 1683, 3056, 3562, 3633, 4163, 4170, 4497, 4566
BsaWI WCCGGW 3 cut(s) 339, 1750, 1783
BsaXI ACNNNNNCTCC 6 cut(s) 1679, 1709, 2097, 2127, 3340, 3370
Bse118I RCCGGY 1 cut(s) 339
Bse1I ACTGG 9 cut(s) 271, 1042, 1731, 1821, 2415, 2540, 2749, 3068, 4321
Bse21I CCTNAGG 1 cut(s) 3840
Bse3DI GCAATG 5 cut(s) 1650, 2406, 3148, 3561, 4283
Bse8I GATNNNNATC 2 cut(s) 1608, 3195
BseAI TCCGGA 2 cut(s) 1750, 1783
BseBI CCWGG 6 cut(s) 80, 1323, 3634, 4112, 4272, 4299
BseDI CCNNGG 9 cut(s) 1069, 1683, 3056, 3562, 3633, 4163, 4170, 4497, 4566
BseGI GGATG 8 cut(s) 506, 1225, 1369, 1792, 3066, 3278, 3802, 4275
BseJI GATNNNNATC 2 cut(s) 1608, 3195
BseMI GCAATG 5 cut(s) 1650, 2406, 3148, 3561, 4283
BseNI ACTGG 9 cut(s) 271, 1042, 1731, 1821, 2415, 2540, 2749, 3068, 4321
BseRI GAGGAG 3 cut(s) 1573, 3857, 4251
BseSI GKGCMC 2 cut(s) 1069, 3544
BseYI CCCAGC 1 cut(s) 1889
BsgI GTGCAG 3 cut(s) 317, 3568, 4475
Bsh1236I CGCG 2 cut(s) 177, 198
Bsh1285I CGRYCG 2 cut(s) 340, 4366
BshNI GGYRCC 1 cut(s) 3704
BshTI ACCGGT 1 cut(s) 339
BsiEI CGRYCG 2 cut(s) 340, 4366
BsiHKAI GWGCWC 2 cut(s) 2041, 3544
BsiSI CCGG 5 cut(s) 340, 1049, 1751, 1784, 3562
BslFI GGGAC 3 cut(s) 726, 3651, 4344
BsmAI GTCTC 9 cut(s) 278, 1682, 1937, 2077, 2548, 3315, 4204, 4454, 4579
BsmBI CGTCTC 4 cut(s) 1682, 3315, 4204, 4454
BsmFI GGGAC 3 cut(s) 726, 3651, 4344
BsmI GAATGC 2 cut(s) 3309, 4093
Bsp119I TTCGAA 1 cut(s) 726
Bsp1286I GDGCHC 4 cut(s) 1069, 1136, 2041, 3544
Bsp13I TCCGGA 2 cut(s) 1750, 1783
Bsp1407I TGTACA 1 cut(s) 3762
Bsp1720I GCTNAGC 2 cut(s) 696, 1833
Bsp19I CCATGG 1 cut(s) 1069
BspEI TCCGGA 2 cut(s) 1750, 1783
BspFNI CGCG 2 cut(s) 177, 198
BspHI TCATGA 2 cut(s) 991, 2848
BspMI ACCTGC 4 cut(s) 69, 622, 3384, 4052
BspT104I TTCGAA 1 cut(s) 726
BspT107I GGYRCC 1 cut(s) 3704
BsrDI GCAATG 5 cut(s) 1650, 2406, 3148, 3561, 4283
BsrFI RCCGGY 1 cut(s) 339
BsrGI TGTACA 1 cut(s) 3762
BsrI ACTGG 9 cut(s) 271, 1042, 1731, 1821, 2415, 2540, 2749, 3068, 4321
BssAI RCCGGY 1 cut(s) 339
BssECI CCNNGG 9 cut(s) 1069, 1683, 3056, 3562, 3633, 4163, 4170, 4497, 4566
BssNAI GTATAC 1 cut(s) 882
BssNI GRCGYC 1 cut(s) 38
BssSI CACGAG 1 cut(s) 3354
BssT1I CCWWGG 6 cut(s) 1069, 1683, 3056, 4163, 4170, 4497
Bst1107I GTATAC 1 cut(s) 882
Bst2BI CACGAG 1 cut(s) 3354
Bst2UI CCWGG 6 cut(s) 80, 1323, 3634, 4112, 4272, 4299
Bst6I CTCTTC 2 cut(s) 1146, 2315
BstACI GRCGYC 1 cut(s) 38
BstAPI GCANNNNNTGC 5 cut(s) 644, 2064, 2501, 4453, 4508
BstAUI TGTACA 1 cut(s) 3762
BstBAI YACGTR 1 cut(s) 1532
BstBI TTCGAA 1 cut(s) 726
BstDSI CCRYGG 1 cut(s) 1069
BstENI CCTNNNNNAGG 2 cut(s) 89, 158
BstF5I GGATG 8 cut(s) 506, 1225, 1369, 1792, 3066, 3278, 3802, 4275
BstFNI CGCG 2 cut(s) 177, 198
BstH2I RGCGCY 2 cut(s) 211, 1844
BstHHI GCGC 4 cut(s) 177, 210, 1843, 1878
BstMAI GTCTC 9 cut(s) 278, 1682, 1937, 2077, 2548, 3315, 4204, 4454, 4579
BstMCI CGRYCG 2 cut(s) 340, 4366
BstNI CCWGG 6 cut(s) 80, 1323, 3634, 4112, 4272, 4299
BstNSI RCATGY 4 cut(s) 1315, 2351, 2596, 3594
BstSCI CCNGG 8 cut(s) 78, 1048, 1321, 3561, 3632, 4110, 4270, 4297
BstSFI CTRYAG 7 cut(s) 678, 1977, 2436, 3030, 3160, 3981, 4231
BstSLI GKGCMC 2 cut(s) 1069, 3544
BstSNI TACGTA 1 cut(s) 1532
BstUI CGCG 2 cut(s) 177, 198
BstV2I GAAGAC 3 cut(s) 2820, 3849, 4259
BstX2I RGATCY 5 cut(s) 3064, 3783, 3899, 4204, 4430
BstXI CCANNNNNNTGG 2 cut(s) 1258, 3779
BstYI RGATCY 5 cut(s) 3064, 3783, 3899, 4204, 4430
BstZ17I GTATAC 1 cut(s) 882
Bsu36I CCTNAGG 1 cut(s) 3840
BsuI GTATCC 1 cut(s) 2319
BtgI CCRYGG 1 cut(s) 1069
BtsCI GGATG 8 cut(s) 506, 1225, 1369, 1792, 3066, 3278, 3802, 4275
BtsI GCAGTG 2 cut(s) 422, 3556
BtsIMutI CAGTG 8 cut(s) 422, 719, 1035, 1738, 3456, 3542, 3556, 4521
BveI ACCTGC 4 cut(s) 69, 622, 3384, 4052
CaiI CAGNNNCTG 5 cut(s) 721, 800, 2261, 3341, 4117
CciI TCATGA 2 cut(s) 991, 2848
CfoI GCGC 4 cut(s) 177, 210, 1843, 1878
Cfr10I RCCGGY 1 cut(s) 339
CseI GACGC 2 cut(s) 27, 34
CsiI ACCWGGT 2 cut(s) 78, 4110
Csp6I GTAC 7 cut(s) 46, 202, 240, 1895, 2620, 3170, 3763
CspAI ACCGGT 1 cut(s) 339
CviQI GTAC 7 cut(s) 46, 202, 240, 1895, 2620, 3170, 3763
DraI TTTAAA 1 cut(s) 2532
DraIII CACNNNGTG 1 cut(s) 4114
DrdI GACNNNNNNGTC 1 cut(s) 31
DriI GACNNNNNGTC 3 cut(s) 64, 1950, 4217
DseDI GACNNNNNNGTC 1 cut(s) 31
EaeI YGGCCR 4 cut(s) 2077, 2289, 2705, 2894
Eam1104I CTCTTC 2 cut(s) 1146, 2315
Eam1105I GACNNNNNGTC 3 cut(s) 64, 1950, 4217
EarI CTCTTC 2 cut(s) 1146, 2315
EciI GGCGGA 1 cut(s) 118
Eco105I TACGTA 1 cut(s) 1532
Eco130I CCWWGG 6 cut(s) 1069, 1683, 3056, 4163, 4170, 4497
Eco147I AGGCCT 2 cut(s) 2753, 3352
Eco24I GRGCYC 1 cut(s) 1136
Eco32I GATATC 3 cut(s) 1201, 3679, 3694
Eco47I GGWCC 6 cut(s) 82, 151, 1046, 2009, 2990, 4385
Eco57I CTGAAG 5 cut(s) 107, 431, 857, 2030, 4176
Eco81I CCTNAGG 1 cut(s) 3840
EcoNI CCTNNNNNAGG 2 cut(s) 89, 158
EcoO109I RGGNCCY 3 cut(s) 2990, 3872, 4041
EcoRI GAATTC 2 cut(s) 1405, 3025
EcoRII CCWGG 6 cut(s) 78, 1321, 3632, 4110, 4270, 4297
EcoRV GATATC 3 cut(s) 1201, 3679, 3694
EcoT14I CCWWGG 6 cut(s) 1069, 1683, 3056, 4163, 4170, 4497
EcoT22I ATGCAT 5 cut(s) 1354, 1529, 2278, 2353, 3280
EcoT38I GRGCYC 1 cut(s) 1136
ErhI CCWWGG 6 cut(s) 1069, 1683, 3056, 4163, 4170, 4497
Esp3I CGTCTC 4 cut(s) 1682, 3315, 4204, 4454
FalI AAGNNNNNCTT 6 cut(s) 218, 250, 3502, 3534, 4065, 4097
FaqI GGGAC 3 cut(s) 726, 3651, 4344
FauI CCCGC 1 cut(s) 359
FauNDI CATATG 4 cut(s) 532, 825, 1633, 3609
FbaI TGATCA 3 cut(s) 1177, 3196, 3421
FblI GTMKAC 1 cut(s) 881
FokI GGATG 8 cut(s) 493, 1232, 1356, 1799, 3073, 3265, 3809, 4262
FriOI GRGCYC 1 cut(s) 1136
GlaI GCGC 4 cut(s) 176, 209, 1842, 1877
GsaI CCCAGC 1 cut(s) 1893
GsuI CTGGAG 4 cut(s) 2523, 3616, 4320, 4338
HaeII RGCGCY 2 cut(s) 211, 1844
HapII CCGG 5 cut(s) 340, 1049, 1751, 1784, 3562
HgaI GACGC 2 cut(s) 27, 34
HhaI GCGC 4 cut(s) 177, 210, 1843, 1878
Hin1I GRCGYC 1 cut(s) 38
Hin6I GCGC 4 cut(s) 175, 208, 1841, 1876
HinP1I GCGC 4 cut(s) 175, 208, 1841, 1876
HincII GTYRAC 8 cut(s) 1026, 1264, 1955, 2659, 2772, 2842, 3516, 4314
HindII GTYRAC 8 cut(s) 1026, 1264, 1955, 2659, 2772, 2842, 3516, 4314
HindIII AAGCTT 2 cut(s) 2815, 3221
HpaI GTTAAC 3 cut(s) 1264, 2772, 4314
HpaII CCGG 5 cut(s) 340, 1049, 1751, 1784, 3562
Hpy99I CGWCG 5 cut(s) 28, 43, 70, 143, 4344
HpyCH4IV ACGT 5 cut(s) 258, 1531, 1621, 1691, 4339
HpySE526I ACGT 5 cut(s) 258, 1531, 1621, 1691, 4339
Hsp92I GRCGYC 1 cut(s) 38
HspAI GCGC 4 cut(s) 175, 208, 1841, 1876
Kpn2I TCCGGA 2 cut(s) 1750, 1783
Ksp22I TGATCA 3 cut(s) 1177, 3196, 3421
KspAI GTTAAC 3 cut(s) 1264, 2772, 4314
LmnI GCTCC 6 cut(s) 105, 601, 2700, 3564, 3587, 4199
MabI ACCWGGT 2 cut(s) 78, 4110
MaeII ACGT 5 cut(s) 258, 1531, 1621, 1691, 4339
MfeI CAATTG 2 cut(s) 1181, 1665
MflI RGATCY 5 cut(s) 3064, 3783, 3899, 4204, 4430
MhlI GDGCHC 4 cut(s) 1069, 1136, 2041, 3544
MlsI TGGCCA 4 cut(s) 2079, 2291, 2707, 2896
MluNI TGGCCA 4 cut(s) 2079, 2291, 2707, 2896
MlyI GAGTC 3 cut(s) 176, 3332, 4410
MmeI TCCRAC 8 cut(s) 21, 816, 2577, 2965, 3326, 3465, 4092, 4384
Mox20I TGGCCA 4 cut(s) 2079, 2291, 2707, 2896
Mph1103I ATGCAT 5 cut(s) 1354, 1529, 2278, 2353, 3280
MroI TCCGGA 2 cut(s) 1750, 1783
MroXI GAANNNNTTC 3 cut(s) 1057, 1332, 2918
MscI TGGCCA 4 cut(s) 2079, 2291, 2707, 2896
MslI CAYNNNNRTG 2 cut(s) 621, 2362
Msp20I TGGCCA 4 cut(s) 2079, 2291, 2707, 2896
MspA1I CMGCKG 5 cut(s) 1661, 1927, 2146, 2396, 3734
MspI CCGG 5 cut(s) 340, 1049, 1751, 1784, 3562
MspR9I CCNGG 8 cut(s) 80, 1050, 1323, 3563, 3634, 4112, 4272, 4299
MunI CAATTG 2 cut(s) 1181, 1665
Mva1269I GAATGC 2 cut(s) 3309, 4093
MvaI CCWGG 6 cut(s) 80, 1323, 3634, 4112, 4272, 4299
MvnI CGCG 2 cut(s) 177, 198
NciI CCSGG 2 cut(s) 1050, 3563
NcoI CCATGG 1 cut(s) 1069
NdeI CATATG 4 cut(s) 532, 825, 1633, 3609
NmuCI GTSAC 7 cut(s) 179, 437, 469, 1697, 2398, 2420, 4106
NsiI ATGCAT 5 cut(s) 1354, 1529, 2278, 2353, 3280
NspI RCATGY 4 cut(s) 1315, 2351, 2596, 3594
NspV TTCGAA 1 cut(s) 726
PaeI GCATGC 2 cut(s) 1315, 2351
PagI TCATGA 2 cut(s) 991, 2848
PaqCI CACCTGC 1 cut(s) 3384
PceI AGGCCT 2 cut(s) 2753, 3352
PciI ACATGT 1 cut(s) 2592
PcsI WCGNNNNNNNCGW 2 cut(s) 1272, 1618
PctI GAATGC 2 cut(s) 3309, 4093
PdmI GAANNNNTTC 3 cut(s) 1057, 1332, 2918
PflMI CCANNNNNTGG 4 cut(s) 800, 3341, 3580, 3819
PinAI ACCGGT 1 cut(s) 339
Ple19I CGATCG 1 cut(s) 4366
PleI GAGTC 3 cut(s) 176, 3332, 4409
PpsI GAGTC 3 cut(s) 176, 3332, 4409
Ppu21I YACGTR 1 cut(s) 1532
PpuMI RGGWCCY 1 cut(s) 2990
PscI ACATGT 1 cut(s) 2592
Psp5II RGGWCCY 1 cut(s) 2990
Psp6I CCWGG 6 cut(s) 78, 1321, 3632, 4110, 4270, 4297
PspFI CCCAGC 1 cut(s) 1889
PspGI CCWGG 6 cut(s) 78, 1321, 3632, 4110, 4270, 4297
PspPPI RGGWCCY 1 cut(s) 2990
PstNI CAGNNNCTG 5 cut(s) 721, 800, 2261, 3341, 4117
PsuI RGATCY 5 cut(s) 3064, 3783, 3899, 4204, 4430
PvuI CGATCG 1 cut(s) 4366
PvuII CAGCTG 3 cut(s) 1661, 2146, 2396
RsaI GTAC 7 cut(s) 47, 203, 241, 1896, 2621, 3171, 3764
RsaNI GTAC 7 cut(s) 46, 202, 240, 1895, 2620, 3170, 3763
RseI CAYNNNNRTG 2 cut(s) 621, 2362
ScaI AGTACT 1 cut(s) 47
SchI GAGTC 3 cut(s) 176, 3332, 4410
ScrFI CCNGG 8 cut(s) 80, 1050, 1323, 3563, 3634, 4112, 4272, 4299
SduI GDGCHC 4 cut(s) 1069, 1136, 2041, 3544
SexAI ACCWGGT 2 cut(s) 78, 4110
SfcI CTRYAG 7 cut(s) 678, 1977, 2436, 3030, 3160, 3981, 4231
SfuI TTCGAA 1 cut(s) 726
SinI GGWCC 6 cut(s) 82, 151, 1046, 2009, 2990, 4385
SmiMI CAYNNNNRTG 2 cut(s) 621, 2362
SmlI CTYRAG 3 cut(s) 707, 1879, 2545
SmoI CTYRAG 3 cut(s) 707, 1879, 2545
SnaBI TACGTA 1 cut(s) 1532
SphI GCATGC 2 cut(s) 1315, 2351
SseBI AGGCCT 2 cut(s) 2753, 3352
SspI AATATT 1 cut(s) 1828
StuI AGGCCT 2 cut(s) 2753, 3352
StyD4I CCNGG 8 cut(s) 78, 1048, 1321, 3561, 3632, 4110, 4270, 4297
StyI CCWWGG 6 cut(s) 1069, 1683, 3056, 4163, 4170, 4497
TaiI ACGT 5 cut(s) 261, 1534, 1624, 1694, 4342
TaqII GACCGA 2 cut(s) 3378, 3495
TatI WGTACW 3 cut(s) 45, 3169, 3762
TauI GCSGC 5 cut(s) 111, 213, 1927, 4061, 4514
TscAI CASTG 8 cut(s) 422, 726, 1042, 1738, 3463, 3549, 3556, 4528
TseFI GTSAC 7 cut(s) 179, 437, 469, 1697, 2398, 2420, 4106
Tsp45I GTSAC 7 cut(s) 179, 437, 469, 1697, 2398, 2420, 4106
TspGWI ACGGA 3 cut(s) 227, 4228, 4268
TspRI CASTG 8 cut(s) 422, 726, 1042, 1738, 3463, 3549, 3556, 4528
Van91I CCANNNNNTGG 4 cut(s) 800, 3341, 3580, 3819
VneI GTGCAC 1 cut(s) 3540
VpaK11BI GGWCC 6 cut(s) 82, 151, 1046, 2009, 2990, 4385
XagI CCTNNNNNAGG 2 cut(s) 89, 158
XapI RAATTY 4 cut(s) 1405, 1743, 3025, 3724
XbaI TCTAGA 2 cut(s) 2569, 3526
XceI RCATGY 4 cut(s) 1315, 2351, 2596, 3594
XcmI CCANNNNNNNNNTGG 2 cut(s) 1076, 3342
XmiI GTMKAC 1 cut(s) 881
XmnI GAANNNNTTC 3 cut(s) 1057, 1332, 2918
ZrmI AGTACT 1 cut(s) 47
Zsp2I ATGCAT 5 cut(s) 1354, 1529, 2278, 2353, 3280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.