Prupe.2G065200_v2.0.a1

Phosphoinositide 3-kinase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0001323
Physical Location & Seq
Forward (+)
8947552 .. 8956125
8574 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G065200.1

Sequence Viewer

Length: 4665 bp
ATGGGCAACAAGATCGCTCGAACGACTCAAGTCTCGGCGTCGGAATACTACCTGCACGACCTGCCGTCCTCCTATAACCTCGTCCTGAAGGAGGTTCTAGGCCGAGGCCGCTTCTTCAAGTCCATTGAGTGCAAGCACGACGAGGGTTTGGTCCTCGTCAAGGTCTACTTCAAGCGCGGCGACTCCATCGATCTCCGTGAGTACGAGCGCAGGCTCTTCCACATCAAGGAGACCTTCCGTGCTCTTGATCACCCTCACGTTTGGCCCTTCCAGTTTTGGCAAGAGACAGATAAAGCCGCTTACCTTGTAAGGCAATACTTCTTCAATAATCTGCATGACCGATTAAGCACACGACCTTTTCTCAGTCTCATCGAGAAGAAATGGTTGGCTTTTCAGTTACTTCTTGCTCTGAAGCAGTGCCACGACAAAGGCATATGTCATGGCGATATTAAGTGTGAGAATGTGTTGGTGACTTCTTGGAATTGGCTCTACCTTGCTGACTTTGCATCTTTCAAACCCACTTACATACCATACGACGACCCTTCAGATTTCTCATTTTTCTTTGACACTGGAGGAAGAAGGCTTTGTTATCTTGCTCCTGAGAGATTTTATGAGCATGGAGGTGAGATGCAGGTTGCACAAGATGCACCATTGAGGCCATCTATGGACATCTTTGCTGTAGGGTGTGTAATTGCTGAGCTTTTCCTTGAGGGTCAGCCACTATTCGAACTCTCTCAACTTCTTGCTTATCGTAGAGGGCAATATGATCCTACCCAACTTCTTGAAAAGATACCAGATTCTGGGATTCGCAAGATGATACTTCATATGATTCAGTTGGAACCAGAGTTGCGACTCTCTGCTGATAGCTACCTGCAAGAGTATACCACCATTGTGTTTCCAAGTTACTTCTCTCCGTTTCTGCATAATTTTCATTGTTTCTGGAATCCACTTCATTCAGATATGAGGGTTGCACTTTGCCAGAGTGTTTTCCATGAGATACTTAAACAAATGATGAGCAATAGGTCAACTGAGGACACTGGAACTGGACTTGGAACTCCTCCAAATGCCAATGCCATTAGTGATAAAACTTCTCAGGAGGTCGTTACAATGCAGAACAAGAATTTTGCAAAGGGCTCGATTAGAAAGAGAGAAGAGATAGGTAAAGGCTTAAAATGTGATCAATTTGAACTTCTTGGTGATATCAATACCCTTCTCAGGGATGTTAAACAAAGTAATCATTATTCTGTTTCCAAGCCAGTGTTAGACGATAATCCTGATTCTACATTTTCCCAAAATCTCGGAAACTATGGGATGCAGTCTCCTGGTGAATTACTTCAGTCTATCTCCAATGCATTTAGAAGAAATGATCATCCATTTATGAAAAAGATTACACTAAATGATTTGAATTCATTGATGTCCAAGTATGACAGCCAATCAGATACCTTTGGCATGCCTTTTCTGCCGTTGCCTGAAGACAGTATGAGATGTGAGGGGATGGTTCTGATTACCTCTTTGCTCTGTTCCTGCATACGCAATGTAAAATTGCCTCATTTAAGGAGGAGGGCCATACTGTTGCTCAAATCGTCTGCCTTGTATATTGATGACGAAGATCGTTTGCAACGTGTAATTCCATATGTTGTTGCAATGCTTTCAGATCCAGCTGCAATTGTGCGTTGTGCTGCCTTGGAGACGTTGTGTGACATTCTCCCTTTAGTCCGAGATTTTCCTCCCAGTGATGCAAAAATTTTTCCAGAGTATATTCTGCCAATGCTCTCTATGCTTCCTGATGATCCAGAGGAAAGCGTGAGGATATGTTATGCCAGTAATATAGCTAAACTGGCACTAACTGCTTATGGGTTCTTGATTCACTCAATAAGCTTGAGTGAGGCTGGGGTACTTGATGAATTAAGTTCTGCCAAAAAGCCACTGGCATCATCTAGTGAGACATCTGGTCAACTTCAGAGGGTAAACAGCGATGCACAGCTTGCAATGTTGAGGAAGTCCATAGCTGAGGTCATTCAAGAACTTGTTATGGGTCCAAAGCAAACTCCAAATATCAGGAGGGCACTCCTTCAGGACATTAGCAACCTATGCTGCTTCTTTGGCCAGAGACAGAGTAATGACTTTTTGTTACCCATTCTCCCCGCTTTTCTGAATGATCGCGATGAGCAGCTTAGAGCAGTATTCTATGGGCAAATTGTTTATGTATGCTTCTTTGTGGGTCAAAGAAGTGTGGAAGAATATCTTTTACCTTATATTGAGCAGGCAGTAAGTGATGTTACAGAGGCTGTCATTGTCAATGCATTGGACTGTTTGGCCATTTTGTGCAAAAGTGGTTTCTTGCGAAAGAGGATACTTCTTGAAATGATTGAGCGTGCCTTTCCATTGTTATGTTATCCTAGTCAATGGGTAAGAAGGTCAGCTGTCACTTTTATTGCGGCCAGCAGTGACTGCTTAGGTGCCGTAGATTCCTATGTTTTTCTTGCCCCTGTTATTCGGCCTCTACTTCGCAGGCAGCCAGCATCTCTAGCTTCTGAAAAGGCTCTCCTTGCATGTTTAAAACCTCCAGTCTCAAGACAGGTTTTTTACCAAGTTCTAGAAAATGCAAGGAGTTCAGACATGTTGGAAAGACAGAGAAAGATATGGTACAACTCCTGGCCTCAATCTAAACAATGGGAAAGTGTGGATTTACTTCCGAAAGGGGTTGAGGAATTGAGTTCAACGAGGAACTGGCCCGACAAGCAACAAAATCCCGAGAATCAAAAACTTACTGGAAAGGCATTGCAACAAGCAGAGCTAACTGAATGTGAAGATGGTGAGGCTAAGTTGAGAAGTATGGGAAGCTTTACACGTGCTTCTAGTACAGTTGACATTCATGATCCTTTGTCCTCTGAAAAGTTGCAATTTTCAGGCTTTATGTGGCCACAGGGAAGTGGTGTGAATAGCTTCATGTGCGATAAATCATCAGTAGGCATACCTTTATACTCCTTTAGCATGGACAGGCGAGCAGTGGGAGTTCCTCCTGCAGCATCTGATTCTCCATCTCAAGTGAATTCTGTAGGACTTGGTGCATCATCCATGCCTTGGATGGATCCAGTAAATAAATCATTTAGTTTGGCTAGTTCTGTTCCAGCACCTAAGCTTGTGTCGGGATCATTCAACATGAGTAGTGGTTCCAAACAGTTTTATAGAGTCGTGCATGAGCCAGATGGTAGGGATAATGATCAAACAGCCTTTGCTAGTAGCAAGCTTCAAGATATGGGATTATCTGGCACTTCAAAAGGAAGTTCTATAGCTGCGGAAGATGCATCACCTCCAAGTGATATAACTGGATTGCCCTCTTCTGCACGAAATTCATCAATTCCAGATTCTGGTTGGAGGCCCCGTGGGGTGCTGGTTGCACATCTGCAAGAACATCGGTCTGCTGTCAATGACATAGCCATTTCAACTGATCATAGTTTTTTTGTGAGTGCATCTGATGATTCCACTGTCAAGGTGTGGGATTCTAGAAAGTTGGAAAAGGACATATCATTTCGGTCGAGGTTAACTTATCACCTTGAGGGAAGCCGTGCACTGTGCACTGCAATGCTCCGGGGCTCTGCTCAAGTTGTAGTTGGAGCATGTGATGGCATGATACATATGTTTTCTGTTGATTACATCTCCAGAGGTCTTGGTAATGTTGTTGAAAAGTATTCGGGTGTTGCTGATATCAAGAAGAAGGATATTAAGGAAGGTGCCATACTCAGCCTTTTGAATTTCTCTGCTGATAACTGTACAAATCAGATGGTTATGTACAGCACCCAAAATTGTGGGATCCATCTTTGGGATACAAGAATGAATACCAATTCTTGGACATTGAGAGCAACTCCTGAAGAGGGCTATGTTTCTTCACTGGTAACAGGACCTTGTGAAAATTGGTTTGTGTCCGGATCTTCAAGGGGTGTGCTTACTCTTTGGGATATGAGGTTCCTTATTCCTGTGAACTCATGGCAGTATTCTGCTGTTTGCCCTATTGAGAAGATGTGTCTTTTTCTTCCTCCTCCAAATACTTCTGCCTCTGCTGCTGCAAGACCCCTTGTTTATGTTGCTGCAGGTTGCAATGAAGTTTCTCTTTGGAATGCTGAAAATGGAAGCTGCCACCAGGTGTTGAGAGTGGCAAGTTATGAAAGTGATGCTGAAACATCTGAAGTGCCTTGGGCCTTGGCTAGATCATCAAGTAAAAATTCTAAACCAGATCTGAGACGGAATGTCAATCCCCACTACAGAGTTGATGAACTAAACGAACCCCCTCCTCGTCTTCCTGGCATCCGTTCATTGCTGCCCTTACCTGGGGGTGATTTGTTAACTGGAGGAACCGACTTGAAAATACGTCGATGGGATCATTACAGTCCTGATCGAAGTTACTCCATCTGTGGGCCAAACTTGAAGGGAGTTGGAAATGATGATTTCTATGCGACAAGATCTAGTTTTGGTGTGCAAGTTGTGCAGGAGACAAAGAGACGACCTCTAACGTCAAAGCTCACAGCCAAGGCAGTACTTGCGGCTGCTGCCACTGATTCTGCTGGCTGCCACCGTGATTCTATTCTGTCATTGGCCTCGGTTAAGTTAAACCAGAGACACTTAATATCGAGCAGTAGAGATGGGGCAATCAAGGTTTGGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000011 GO:0000151 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005737 GO:0005768 GO:0005770 GO:0005942 GO:0006355 GO:0006357 GO:0006464 GO:0006468 GO:0006605 GO:0006623 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006886 GO:0006914 GO:0006996 GO:0007033 GO:0007034 GO:0007154 GO:0007165 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009555 GO:0009846 GO:0009856 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010008 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0016236 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019219 GO:0019222 GO:0019538 GO:0019898 GO:0022414 GO:0023052 GO:0030242 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031410 GO:0031461 GO:0031967 GO:0031975 GO:0031982 GO:0032182 GO:0032501 GO:0032502 GO:0032507 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0033036 GO:0033365 GO:0034067 GO:0034243 GO:0034271 GO:0034272 GO:0034613 GO:0035032 GO:0035556 GO:0036211 GO:0042886 GO:0043130 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0044706 GO:0045053 GO:0045184 GO:0045185 GO:0045324 GO:0045893 GO:0045935 GO:0045944 GO:0046907 GO:0048015 GO:0048017 GO:0048229 GO:0048308 GO:0048518 GO:0048522 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051235 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051651 GO:0051704 GO:0051716 GO:0060255 GO:0061695 GO:0061919 GO:0065007 GO:0065008 GO:0070727 GO:0071561 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0080008 GO:0080090 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0120095 GO:0140096 GO:1901564 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04136 ko04138 ko04140 ko04371 map04136 map04138 map04140 map04371
Pfam Domains
Protein Families
HEAT Pkinase WD40

Protein Analysis

1555

Amino Acids

173.88

Weight (kDa)

6.79

Isoelectric Point (pI)

52.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 5 cut(s) 60, 69, 622, 879, 4082
AccB1I GGYRCC 2 cut(s) 2459, 3734
AccB7I CCANNNNNTGG 4 cut(s) 800, 3084, 3371, 3849
AccI GTMKAC 2 cut(s) 165, 881
AccII CGCG 2 cut(s) 177, 2163
AccIII TCCGGA 1 cut(s) 3926
AciI CCGC 7 cut(s) 109, 177, 297, 2145, 2438, 3299, 4541
AcoI YGGCCR 4 cut(s) 2104, 2316, 2439, 2921
AcsI RAATTY 7 cut(s) 1120, 1405, 1743, 3052, 3352, 3754, 4222
AcuI CTGAAG 9 cut(s) 107, 431, 528, 1319, 1491, 1943, 2057, 3891, 4206
AcvI CACGTG 1 cut(s) 2852
AcyI GRCGYC 1 cut(s) 38
AdeI CACNNNGTG 1 cut(s) 4144
AfaI GTAC 7 cut(s) 203, 1896, 2648, 2863, 3775, 3794, 4536
AflIII ACRYGT 3 cut(s) 1621, 2619, 2849
AhdI GACNNNNNGTC 3 cut(s) 64, 1950, 4247
AjnI CCWGG 5 cut(s) 1321, 2654, 4140, 4300, 4327
AjuI GAANNNNNNNTTGG 2 cut(s) 368, 400
AleI CACNNNNGTG 1 cut(s) 890
AloI GAACNNNNNNTCC 2 cut(s) 3950, 3982
Alw21I GWGCWC 3 cut(s) 244, 3574, 3581
Alw44I GTGCAC 2 cut(s) 3570, 3577
AlwNI CAGNNNCTG 5 cut(s) 800, 2288, 2451, 3032, 3371
Ama87I CYCGRG 1 cut(s) 2753
Aor13HI TCCGGA 1 cut(s) 3926
ApaLI GTGCAC 2 cut(s) 3570, 3577
ApoI RAATTY 7 cut(s) 1120, 1405, 1743, 3052, 3352, 3754, 4222
Asp700I GAANNNNTTC 2 cut(s) 1332, 2945
AspLEI GCGC 2 cut(s) 177, 210
AspS9I GGNCC 9 cut(s) 151, 264, 1563, 2036, 2733, 3381, 3902, 4197, 4415
AsuC2I CCSGG 1 cut(s) 3593
AsuHPI GGTGA 9 cut(s) 242, 481, 635, 1208, 1337, 2828, 3303, 3545, 4346
AsuII TTCGAA 1 cut(s) 726
AvaI CYCGRG 1 cut(s) 2753
AvaII GGWCC 3 cut(s) 151, 2036, 3902
BaeGI GKGCMC 3 cut(s) 2068, 3574, 3581
BaeI ACNNNNGTAYC 2 cut(s) 3626, 3659
BalI TGGCCA 3 cut(s) 2106, 2318, 2923
BamHI GGATCC 2 cut(s) 3091, 3813
BanI GGYRCC 2 cut(s) 2459, 3734
BanII GRGCYC 2 cut(s) 1136, 3599
BarI GAAGNNNNNNTAC 2 cut(s) 3723, 3755
BbrPI CACGTG 1 cut(s) 2852
BbsI GAAGAC 2 cut(s) 1479, 4289
Bbv12I GWGCWC 3 cut(s) 244, 3574, 3581
BbvCI CCTCAGC 1 cut(s) 2010
BceAI ACGGC 4 cut(s) 49, 1447, 2447, 3552
BcgI CGANNNNNNTGC 5 cut(s) 29, 1116, 1150, 3398, 3432
BciT130I CCWGG 5 cut(s) 1323, 2656, 4142, 4302, 4329
BciVI GTATCC 2 cut(s) 2346, 3820
BclI TGATCA 5 cut(s) 247, 1177, 1366, 3223, 3451
BcnI CCSGG 1 cut(s) 3593
BfmI CTRYAG 6 cut(s) 678, 3024, 3057, 3291, 4089, 4261
BfuAI ACCTGC 5 cut(s) 60, 69, 622, 879, 4082
BfuI GTATCC 2 cut(s) 2346, 3820
BglII AGATCT 2 cut(s) 4234, 4460
BlpI GCTNAGC 1 cut(s) 696
BmcAI AGTACT 1 cut(s) 4536
Bme1390I CCNGG 6 cut(s) 1323, 2656, 3593, 4142, 4302, 4329
Bme18I GGWCC 3 cut(s) 151, 2036, 3902
BmeRI GACNNNNNGTC 3 cut(s) 64, 1950, 4247
BmeT110I CYCGRG 1 cut(s) 2753
BmgT120I GGNCC 9 cut(s) 151, 264, 1563, 2036, 2733, 3381, 3902, 4197, 4415
BmrFI CCNGG 6 cut(s) 1323, 2656, 3593, 4142, 4302, 4329
BmrI ACTGGG 1 cut(s) 1725
BmuI ACTGGG 1 cut(s) 1725
BoxI GACNNNNGTC 1 cut(s) 29
BpiI GAAGAC 2 cut(s) 1479, 4289
BplI GAGNNNNNCTC 4 cut(s) 3850, 3882, 4489, 4521
BpmI CTGGAG 4 cut(s) 591, 2550, 3646, 4368
Bpu10I CCTNAGC 3 cut(s) 2010, 2455, 3138
Bpu1102I GCTNAGC 1 cut(s) 696
Bpu14I TTCGAA 1 cut(s) 726
BpuEI CTTGAG 7 cut(s) 12, 728, 1900, 2557, 3030, 3578, 3588
BpuMI CCSGG 1 cut(s) 3593
Bsa29I ATCGAT 1 cut(s) 189
BsaAI YACGTR 1 cut(s) 2852
BsaBI GATNNNNATC 1 cut(s) 3222
BsaHI GRCGYC 1 cut(s) 38
BsaI GGTCTC 1 cut(s) 224
BsaWI WCCGGW 1 cut(s) 3926
BsaXI ACNNNNNCTCC 6 cut(s) 1679, 1709, 2124, 2154, 3370, 3400
Bse3DI GCAATG 7 cut(s) 1540, 1650, 1995, 2780, 3591, 4105, 4313
Bse8I GATNNNNATC 1 cut(s) 3222
BseAI TCCGGA 1 cut(s) 3926
BseBI CCWGG 5 cut(s) 1323, 2656, 4142, 4302, 4329
BseCI ATCGAT 1 cut(s) 189
BseGI GGATG 7 cut(s) 1225, 1317, 1369, 1500, 3074, 3093, 4305
BseJI GATNNNNATC 1 cut(s) 3222
BseMI GCAATG 7 cut(s) 1540, 1650, 1995, 2780, 3591, 4105, 4313
BseMII CTCAG 9 cut(s) 376, 591, 687, 1020, 1106, 1228, 2001, 3757, 4229
BseRI GAGGAG 4 cut(s) 1047, 1573, 4029, 4281
BseSI GKGCMC 3 cut(s) 2068, 3574, 3581
BseYI CCCAGC 1 cut(s) 1889
BsgI GTGCAG 3 cut(s) 38, 3330, 4505
Bsh1236I CGCG 2 cut(s) 177, 2163
Bsh1285I CGRYCG 1 cut(s) 3539
BshNI GGYRCC 2 cut(s) 2459, 3734
BshVI ATCGAT 1 cut(s) 189
BsiEI CGRYCG 1 cut(s) 3539
BsiHKAI GWGCWC 3 cut(s) 244, 3574, 3581
BsiHKCI CYCGRG 1 cut(s) 2753
BsiSI CCGG 2 cut(s) 3592, 3927
BsmBI CGTCTC 3 cut(s) 1682, 4234, 4492
BsmI GAATGC 1 cut(s) 4123
Bso31I GGTCTC 1 cut(s) 224
BsoBI CYCGRG 1 cut(s) 2753
Bsp119I TTCGAA 1 cut(s) 726
Bsp1286I GDGCHC 6 cut(s) 244, 1136, 2068, 3574, 3581, 3599
Bsp13I TCCGGA 1 cut(s) 3926
Bsp1407I TGTACA 2 cut(s) 3773, 3792
Bsp1720I GCTNAGC 1 cut(s) 696
Bsp68I TCGCGA 1 cut(s) 2163
BspACI CCGC 7 cut(s) 109, 177, 297, 2145, 2438, 3299, 4541
BspCNI CTCAG 9 cut(s) 375, 592, 688, 1021, 1105, 1227, 2002, 3756, 4230
BspDI ATCGAT 1 cut(s) 189
BspEI TCCGGA 1 cut(s) 3926
BspFNI CGCG 2 cut(s) 177, 2163
BspHI TCATGA 1 cut(s) 2875
BspMAI CTGCAG 2 cut(s) 3028, 4093
BspMI ACCTGC 5 cut(s) 60, 69, 622, 879, 4082
BspQI GCTCTTC 1 cut(s) 221
BspT104I TTCGAA 1 cut(s) 726
BspT107I GGYRCC 2 cut(s) 2459, 3734
BspTNI GGTCTC 1 cut(s) 224
BsrDI GCAATG 7 cut(s) 1540, 1650, 1995, 2780, 3591, 4105, 4313
BsrGI TGTACA 2 cut(s) 3773, 3792
BssNAI GTATAC 1 cut(s) 882
BssNI GRCGYC 1 cut(s) 38
BssT1I CCWWGG 5 cut(s) 1683, 3083, 4193, 4200, 4527
Bst1107I GTATAC 1 cut(s) 882
Bst2UI CCWGG 5 cut(s) 1323, 2656, 4142, 4302, 4329
Bst6I CTCTTC 4 cut(s) 221, 1146, 3346, 3867
BstACI GRCGYC 1 cut(s) 38
BstAPI GCANNNNNTGC 8 cut(s) 61, 644, 1847, 1985, 2091, 2451, 4483, 4538
BstAUI TGTACA 2 cut(s) 3773, 3792
BstBAI YACGTR 1 cut(s) 2852
BstBI TTCGAA 1 cut(s) 726
BstDSI CCRYGG 1 cut(s) 3385
BstENI CCTNNNNNAGG 2 cut(s) 89, 158
BstF5I GGATG 7 cut(s) 1225, 1317, 1369, 1500, 3074, 3093, 4305
BstFNI CGCG 2 cut(s) 177, 2163
BstHHI GCGC 2 cut(s) 177, 210
BstMCI CGRYCG 1 cut(s) 3539
BstNI CCWGG 5 cut(s) 1323, 2656, 4142, 4302, 4329
BstNSI RCATGY 4 cut(s) 1453, 2556, 2623, 3624
BstPAI GACNNNNGTC 1 cut(s) 29
BstSCI CCNGG 6 cut(s) 1321, 2654, 3591, 4140, 4300, 4327
BstSFI CTRYAG 6 cut(s) 678, 3024, 3057, 3291, 4089, 4261
BstSLI GKGCMC 3 cut(s) 2068, 3574, 3581
BstUI CGCG 2 cut(s) 177, 2163
BstV2I GAAGAC 2 cut(s) 1479, 4289
BstX2I RGATCY 6 cut(s) 1654, 3091, 3813, 3929, 4234, 4460
BstXI CCANNNNNNTGG 1 cut(s) 3809
BstYI RGATCY 6 cut(s) 1654, 3091, 3813, 3929, 4234, 4460
BstZ17I GTATAC 1 cut(s) 882
Bsu15I ATCGAT 1 cut(s) 189
BsuI GTATCC 2 cut(s) 2346, 3820
BsuTUI ATCGAT 1 cut(s) 189
BtgI CCRYGG 1 cut(s) 3385
BtgZI GCGATG 2 cut(s) 1989, 2178
BtsCI GGATG 7 cut(s) 1225, 1317, 1369, 1500, 3074, 3093, 4305
BtsI GCAGTG 4 cut(s) 422, 2452, 3015, 3579
BtuMI TCGCGA 1 cut(s) 2163
BveI ACCTGC 5 cut(s) 60, 69, 622, 879, 4082
CaiI CAGNNNCTG 5 cut(s) 800, 2288, 2451, 3032, 3371
CciI TCATGA 1 cut(s) 2875
CfoI GCGC 2 cut(s) 177, 210
Cfr13I GGNCC 9 cut(s) 151, 264, 1563, 2036, 2733, 3381, 3902, 4197, 4415
ClaI ATCGAT 1 cut(s) 189
CseI GACGC 1 cut(s) 27
CsiI ACCWGGT 1 cut(s) 4140
Csp6I GTAC 7 cut(s) 202, 1895, 2647, 2862, 3774, 3793, 4535
CviQI GTAC 7 cut(s) 202, 1895, 2647, 2862, 3774, 3793, 4535
DraI TTTAAA 1 cut(s) 2559
DraIII CACNNNGTG 1 cut(s) 4144
DriI GACNNNNNGTC 3 cut(s) 64, 1950, 4247
EaeI YGGCCR 4 cut(s) 2104, 2316, 2439, 2921
Eam1104I CTCTTC 4 cut(s) 221, 1146, 3346, 3867
Eam1105I GACNNNNNGTC 3 cut(s) 64, 1950, 4247
EarI CTCTTC 4 cut(s) 221, 1146, 3346, 3867
Eco130I CCWWGG 5 cut(s) 1683, 3083, 4193, 4200, 4527
Eco24I GRGCYC 2 cut(s) 1136, 3599
Eco31I GGTCTC 1 cut(s) 224
Eco32I GATATC 2 cut(s) 1201, 3709
Eco47I GGWCC 3 cut(s) 151, 2036, 3902
Eco57I CTGAAG 9 cut(s) 107, 431, 528, 1319, 1491, 1943, 2057, 3891, 4206
Eco72I CACGTG 1 cut(s) 2852
Eco88I CYCGRG 1 cut(s) 2753
EcoNI CCTNNNNNAGG 2 cut(s) 89, 158
EcoO109I RGGNCCY 2 cut(s) 3381, 3902
EcoRI GAATTC 2 cut(s) 1405, 3052
EcoRII CCWGG 5 cut(s) 1321, 2654, 4140, 4300, 4327
EcoRV GATATC 2 cut(s) 1201, 3709
EcoT14I CCWWGG 5 cut(s) 1683, 3083, 4193, 4200, 4527
EcoT22I ATGCAT 3 cut(s) 1354, 2305, 3310
EcoT38I GRGCYC 2 cut(s) 1136, 3599
ErhI CCWWGG 5 cut(s) 1683, 3083, 4193, 4200, 4527
Esp3I CGTCTC 3 cut(s) 1682, 4234, 4492
FalI AAGNNNNNCTT 8 cut(s) 152, 184, 218, 250, 2229, 2261, 4095, 4127
FauI CCCGC 1 cut(s) 2152
FauNDI CATATG 4 cut(s) 434, 825, 1633, 3639
FbaI TGATCA 5 cut(s) 247, 1177, 1366, 3223, 3451
FblI GTMKAC 2 cut(s) 165, 881
FokI GGATG 7 cut(s) 1232, 1324, 1356, 1507, 3061, 3100, 4292
FriOI GRGCYC 2 cut(s) 1136, 3599
GlaI GCGC 2 cut(s) 176, 209
GsaI CCCAGC 1 cut(s) 1893
GsuI CTGGAG 4 cut(s) 591, 2550, 3646, 4368
HapII CCGG 2 cut(s) 3592, 3927
HgaI GACGC 1 cut(s) 27
HhaI GCGC 2 cut(s) 177, 210
Hin1I GRCGYC 1 cut(s) 38
Hin6I GCGC 2 cut(s) 175, 208
HinP1I GCGC 2 cut(s) 175, 208
HincII GTYRAC 5 cut(s) 1026, 1955, 2869, 3546, 4344
HindII GTYRAC 5 cut(s) 1026, 1955, 2869, 3546, 4344
HindIII AAGCTT 4 cut(s) 1876, 2842, 3140, 3248
HpaI GTTAAC 2 cut(s) 3546, 4344
HpaII CCGG 2 cut(s) 3592, 3927
HphI GGTGA 9 cut(s) 242, 481, 635, 1208, 1337, 2828, 3303, 3545, 4346
Hpy99I CGWCG 4 cut(s) 43, 143, 539, 4374
HpyCH4IV ACGT 6 cut(s) 258, 1621, 1691, 2851, 4369, 4511
HpySE526I ACGT 6 cut(s) 258, 1621, 1691, 2851, 4369, 4511
Hsp92I GRCGYC 1 cut(s) 38
HspAI GCGC 2 cut(s) 175, 208
Kpn2I TCCGGA 1 cut(s) 3926
Ksp22I TGATCA 5 cut(s) 247, 1177, 1366, 3223, 3451
KspAI GTTAAC 2 cut(s) 3546, 4344
LguI GCTCTTC 1 cut(s) 221
LmnI GCTCC 3 cut(s) 601, 3594, 3617
MabI ACCWGGT 1 cut(s) 4140
MaeII ACGT 6 cut(s) 258, 1621, 1691, 2851, 4369, 4511
MfeI CAATTG 1 cut(s) 1665
MflI RGATCY 6 cut(s) 1654, 3091, 3813, 3929, 4234, 4460
MhlI GDGCHC 6 cut(s) 244, 1136, 2068, 3574, 3581, 3599
MlsI TGGCCA 3 cut(s) 2106, 2318, 2923
MluNI TGGCCA 3 cut(s) 2106, 2318, 2923
MlyI GAGTC 4 cut(s) 19, 176, 846, 3201
MmeI TCCRAC 7 cut(s) 21, 816, 2604, 3356, 3495, 3595, 4414
Mox20I TGGCCA 3 cut(s) 2106, 2318, 2923
Mph1103I ATGCAT 3 cut(s) 1354, 2305, 3310
MroI TCCGGA 1 cut(s) 3926
MroXI GAANNNNTTC 2 cut(s) 1332, 2945
MscI TGGCCA 3 cut(s) 2106, 2318, 2923
MslI CAYNNNNRTG 4 cut(s) 621, 890, 2389, 3584
Msp20I TGGCCA 3 cut(s) 2106, 2318, 2923
MspA1I CMGCKG 2 cut(s) 1661, 2423
MspI CCGG 2 cut(s) 3592, 3927
MspR9I CCNGG 6 cut(s) 1323, 2656, 3593, 4142, 4302, 4329
MunI CAATTG 1 cut(s) 1665
Mva1269I GAATGC 1 cut(s) 4123
MvaI CCWGG 5 cut(s) 1323, 2656, 4142, 4302, 4329
MvnI CGCG 2 cut(s) 177, 2163
NciI CCSGG 1 cut(s) 3593
NdeI CATATG 4 cut(s) 434, 825, 1633, 3639
NmeAIII GCCGAG 2 cut(s) 14, 128
NmuCI GTSAC 4 cut(s) 469, 1697, 2425, 2447
NruI TCGCGA 1 cut(s) 2163
NsiI ATGCAT 3 cut(s) 1354, 2305, 3310
NspI RCATGY 4 cut(s) 1453, 2556, 2623, 3624
NspV TTCGAA 1 cut(s) 726
OliI CACNNNNGTG 1 cut(s) 890
PaeI GCATGC 1 cut(s) 1453
PagI TCATGA 1 cut(s) 2875
PciI ACATGT 1 cut(s) 2619
PciSI GCTCTTC 1 cut(s) 221
PcsI WCGNNNNNNNCGW 1 cut(s) 1618
PctI GAATGC 1 cut(s) 4123
PdmI GAANNNNTTC 2 cut(s) 1332, 2945
PflMI CCANNNNNTGG 4 cut(s) 800, 3084, 3371, 3849
PleI GAGTC 4 cut(s) 19, 176, 846, 3200
PmaCI CACGTG 1 cut(s) 2852
PmlI CACGTG 1 cut(s) 2852
PpsI GAGTC 4 cut(s) 19, 176, 846, 3200
Ppu21I YACGTR 1 cut(s) 2852
PpuMI RGGWCCY 1 cut(s) 3902
PscI ACATGT 1 cut(s) 2619
PshAI GACNNNNGTC 1 cut(s) 29
Psp5II RGGWCCY 1 cut(s) 3902
Psp6I CCWGG 5 cut(s) 1321, 2654, 4140, 4300, 4327
PspCI CACGTG 1 cut(s) 2852
PspFI CCCAGC 1 cut(s) 1889
PspGI CCWGG 5 cut(s) 1321, 2654, 4140, 4300, 4327
PspPI GGNCC 9 cut(s) 151, 264, 1563, 2036, 2733, 3381, 3902, 4197, 4415
PspPPI RGGWCCY 1 cut(s) 3902
PstI CTGCAG 2 cut(s) 3028, 4093
PstNI CAGNNNCTG 5 cut(s) 800, 2288, 2451, 3032, 3371
PsuI RGATCY 6 cut(s) 1654, 3091, 3813, 3929, 4234, 4460
PvuII CAGCTG 2 cut(s) 1661, 2423
RruI TCGCGA 1 cut(s) 2163
RsaI GTAC 7 cut(s) 203, 1896, 2648, 2863, 3775, 3794, 4536
RsaNI GTAC 7 cut(s) 202, 1895, 2647, 2862, 3774, 3793, 4535
RseI CAYNNNNRTG 4 cut(s) 621, 890, 2389, 3584
SapI GCTCTTC 1 cut(s) 221
Sau96I GGNCC 9 cut(s) 151, 264, 1563, 2036, 2733, 3381, 3902, 4197, 4415
ScaI AGTACT 1 cut(s) 4536
SchI GAGTC 4 cut(s) 19, 176, 846, 3201
ScrFI CCNGG 6 cut(s) 1323, 2656, 3593, 4142, 4302, 4329
SduI GDGCHC 6 cut(s) 244, 1136, 2068, 3574, 3581, 3599
SexAI ACCWGGT 1 cut(s) 4140
SfcI CTRYAG 6 cut(s) 678, 3024, 3057, 3291, 4089, 4261
SfuI TTCGAA 1 cut(s) 726
SinI GGWCC 3 cut(s) 151, 2036, 3902
SmiMI CAYNNNNRTG 4 cut(s) 621, 890, 2389, 3584
SmlI CTYRAG 7 cut(s) 27, 707, 1879, 2572, 3045, 3557, 3603
SmoI CTYRAG 7 cut(s) 27, 707, 1879, 2572, 3045, 3557, 3603
SphI GCATGC 1 cut(s) 1453
SsiI CCGC 7 cut(s) 109, 177, 297, 2145, 2438, 3299, 4541
StyD4I CCNGG 6 cut(s) 1321, 2654, 3591, 4140, 4300, 4327
StyI CCWWGG 5 cut(s) 1683, 3083, 4193, 4200, 4527
TaiI ACGT 6 cut(s) 261, 1624, 1694, 2854, 4372, 4514
TaqI TCGA 9 cut(s) 19, 189, 372, 726, 1136, 3539, 4372, 4396, 4628
TaqII GACCGA 3 cut(s) 354, 3408, 3525
TatI WGTACW 4 cut(s) 2861, 3773, 3792, 4534
TauI GCSGC 5 cut(s) 111, 180, 299, 2441, 4544
TseFI GTSAC 4 cut(s) 469, 1697, 2425, 2447
Tsp45I GTSAC 4 cut(s) 469, 1697, 2425, 2447
TspGWI ACGGA 5 cut(s) 185, 227, 903, 4258, 4298
Van91I CCANNNNNTGG 4 cut(s) 800, 3084, 3371, 3849
VneI GTGCAC 2 cut(s) 3570, 3577
VpaK11BI GGWCC 3 cut(s) 151, 2036, 3902
XagI CCTNNNNNAGG 2 cut(s) 89, 158
XapI RAATTY 7 cut(s) 1120, 1405, 1743, 3052, 3352, 3754, 4222
XbaI TCTAGA 2 cut(s) 2596, 3506
XceI RCATGY 4 cut(s) 1453, 2556, 2623, 3624
XcmI CCANNNNNNNNNTGG 3 cut(s) 1924, 3085, 3372
XmiI GTMKAC 2 cut(s) 165, 881
XmnI GAANNNNTTC 2 cut(s) 1332, 2945
ZrmI AGTACT 1 cut(s) 4536
Zsp2I ATGCAT 3 cut(s) 1354, 2305, 3310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.