Rmu_sc0033274.1_g000001

Phosphoinositide 3-kinase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0033274.1
OG0001323
Physical Location & Seq
Forward (+)
1 .. 2664
2664 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0033274.1_g000001.1.cds

Sequence Viewer

Length: 2664 bp
agggcgatacttttgctgaagtcatctgcattgtatattgatgatgaaaacaggttgcagcgtgtaattccatatgttgttgcaatgctttcagatcaagctgcaattgtgcgttgtgcagccttggagacgttatgtgacattctccctttagttcgagattttcctcctagtgatgcaaaaatttttccagagtatattctgccaatgctttccatgcttcctgatgatacagaggaaagtgtgaggatatgttatgccagtaatatagctaagctggcactaactgcttatggttttttagttcactcaataaccttgagtgaggctggtgtgcttgatgaattaagttctccaaagaagctactggcatcatctagtgagacatctggtcagctccacaaggtaaataatgatgcacagcttgcccagttgaggaagtccatagctgaagtcattcaagagcttgttatgggtcccaagcaaactcccaatatcaggagagcactccttcaggatatcagcaatctatgctgcttctttggccagaggcagagtaatgactttttgttacccattctccctgcttttctgaatgatcgagatgagcagctaagagcagtattctacgggcaaattgtgtatgtatgcttctttgtgggtcaaagaagtgtggaagaatatcttttaccttatattgagcaggctgtaagtgatgctacagaggctgttattgtcaatgcattggactgtttggccattttgtgcaaaagtggttacctgcgcaagaggatactcctggaaatgattgagcgttcatttccattgttatgctatcctagtcaatgggtaagaaggtcggctgttagttttattgcagccagcagtgattgcttgggtgcagtagattcctatgtattccttgctcctgttattcgtccccttcttcgtagacagccagcatctctagcttctgagaaggctctcctttcatgtttgaagcctcctgtctcaagacaggtattttaccaagttctagaaaatgcaaggagttcagacatgttggaaagacagagaaagatatggtacaattcttggcctcaatctaaacaatgggaaagtgtggatttacttcataaaggggttgttgaattgaattcgatgaggagctggacagacgaccaagaaagtcccgagggtcaaaaacattctggaaatgatttgcaacagggaaagctaactgaatgtgatgatggtgaggctaagtttggatgtatgggaagctttacgcgcaatggttcgagcacagttgacattcatgatcctttatcctctgaaaagttgcagtattcgggctttatgtggccacagggaagtactgtgaatagcttcatgtgtgataaatcatcagtagggatacctttgtactcatttagcatggacaggcgagcagtgggagttcctcctgctccatctgactcttcattacaagtgagttctgtaggagttggtgcatcatctatgccatggatggatccagtaaataaatcttttagtttagctagttctgttccagcacccaagctcgtgtcaggatcattcaacatcagcagtggttctaaacaattctacagagtagtacatgaaccagatggtagggataatgaccaaacaacctttgtcaatagcaaatttcaagatatggggttatctagtgctacaaaagcaagttctattactgtggaagatgcatcctctacaagtgatctaacaggattgccctcttctacaagagcttcgtcaattccagattctggttggaggcctcgtggggttttggttgcacacttgcaagagcaccggtctgctgtgaatgacatagccgtatcaactgatcatagcttctttgtgagtgcatctgatgattccactgtcaaggtatgggattcgagaaagttggaaaaggacatctcctttaggtcaagattgacttatcatatggagggaagccgtgcactatgttctgtaatgctccggggctgcgctcaagttgtagttggagcatgtgatggcatgatacatatgttttctgttgattacatttccagaggtctcggtaatgtagttgagaagtattcaggtgttgctgatatcaagaagaaggacactaaagaaggggcaattcttagccttttgaatttctctgctgacaactatgcaaatcagatggttatgtacagtacccaaaattgtgggatccatctttgggatataagaacgaattcagattcttggacattgaaagcaactccggaggagggctatgtatcttccctagtaacaggcccttgtgaaaattggtttgtgtcaggatcttcaaggggtgtgctcactctttgggatatgaggttccttattcctgtgaatacatggcagtattctgctgtttgccccatagagaaaatgtgtcttttccttcctcctccaaatgcttctgtttctgctgctgcaaggccccttgtttatgttgctgcaggttgcaatgaagtttctctttggaatgcagaaaatggcacctgccaccaggtacaatggaatattttattagtttgtgtacagagaagttaccacctatacagctttgaagatatatgcgtagattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000011 GO:0000151 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005737 GO:0005768 GO:0005770 GO:0005942 GO:0006355 GO:0006357 GO:0006464 GO:0006468 GO:0006605 GO:0006623 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006886 GO:0006914 GO:0006996 GO:0007033 GO:0007034 GO:0007154 GO:0007165 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009555 GO:0009846 GO:0009856 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010008 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0016236 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019219 GO:0019222 GO:0019538 GO:0019898 GO:0022414 GO:0023052 GO:0030242 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031410 GO:0031461 GO:0031967 GO:0031975 GO:0031982 GO:0032182 GO:0032501 GO:0032502 GO:0032507 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0033036 GO:0033365 GO:0034067 GO:0034243 GO:0034271 GO:0034272 GO:0034613 GO:0035032 GO:0035556 GO:0036211 GO:0042886 GO:0043130 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0044706 GO:0045053 GO:0045184 GO:0045185 GO:0045324 GO:0045893 GO:0045935 GO:0045944 GO:0046907 GO:0048015 GO:0048017 GO:0048229 GO:0048308 GO:0048518 GO:0048522 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051235 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051651 GO:0051704 GO:0051716 GO:0060255 GO:0061695 GO:0061919 GO:0065007 GO:0065008 GO:0070727 GO:0071561 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0080008 GO:0080090 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0120095 GO:0140096 GO:1901564 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04136 ko04138 ko04140 ko04371 map04136 map04138 map04140 map04371
Pfam Domains
Protein Families
HEAT Pkinase WD40

Protein Analysis

887

Amino Acids

98.12

Weight (kDa)

5.61

Isoelectric Point (pI)

51.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2585
Acc16I TGCGCA 1 cut(s) 785
Acc36I ACCTGC 3 cut(s) 789, 2525, 2585
AccB1I GGYRCC 1 cut(s) 2573
AccB7I CCANNNNNTGG 1 cut(s) 1814
AccI GTMKAC 1 cut(s) 952
AccII CGCG 1 cut(s) 1291
AccIII TCCGGA 1 cut(s) 2311
AclWI GGATC 7 cut(s) 1316, 1529, 1542, 1603, 2251, 2264, 2380
AcoI YGGCCR 3 cut(s) 544, 756, 1364
AcsI RAATTY 5 cut(s) 183, 1156, 1691, 2197, 2281
AcuI CTGAAG 3 cut(s) 38, 471, 497
AfaI GTAC 8 cut(s) 1088, 1378, 1427, 1641, 2237, 2242, 2589, 2616
AfiI CCNNNNNNNGG 5 cut(s) 435, 498, 1814, 2266, 2318
AflIII ACRYGT 1 cut(s) 1059
AgeI ACCGGT 1 cut(s) 1860
AhdI GACNNNNNGTC 1 cut(s) 390
AjnI CCWGG 2 cut(s) 798, 2583
AloI GAACNNNNNNTCC 2 cut(s) 2393, 2425
Alw21I GWGCWC 5 cut(s) 508, 1307, 1860, 2017, 2391
Alw26I GTCTC 4 cut(s) 122, 377, 1015, 2117
Alw44I GTGCAC 1 cut(s) 2013
AlwI GGATC 7 cut(s) 1316, 1529, 1542, 1603, 2251, 2264, 2380
AlwNI CAGNNNCTG 2 cut(s) 728, 1814
Ama87I CYCGRG 1 cut(s) 1193
Aor13HI TCCGGA 1 cut(s) 2311
AoxI GGCC 7 cut(s) 544, 756, 1097, 1364, 1823, 2344, 2513
ApaLI GTGCAC 1 cut(s) 2013
ApoI RAATTY 5 cut(s) 183, 1156, 1691, 2197, 2281
AsiGI ACCGGT 1 cut(s) 1860
Asp700I GAANNNNTTC 3 cut(s) 456, 1388, 2281
AspLEI GCGC 3 cut(s) 786, 1293, 2045
AspS9I GGNCC 3 cut(s) 476, 2345, 2514
AsuC2I CCSGG 1 cut(s) 2036
AsuHPI GGTGA 1 cut(s) 1268
AvaI CYCGRG 1 cut(s) 1193
AvaII GGWCC 1 cut(s) 476
BaeGI GKGCMC 1 cut(s) 2017
BaeI ACNNNNGTAYC 4 cut(s) 1409, 1442, 2069, 2102
BalI TGGCCA 3 cut(s) 546, 758, 1366
BamHI GGATCC 2 cut(s) 1534, 2256
BanI GGYRCC 1 cut(s) 2573
BarI GAAGNNNNNNTAC 2 cut(s) 635, 667
BauI CACGAG 2 cut(s) 1586, 1827
Bbv12I GWGCWC 5 cut(s) 508, 1307, 1860, 2017, 2391
BccI CCATC 7 cut(s) 1247, 1480, 1525, 1646, 2063, 2221, 2268
BceAI ACGGC 2 cut(s) 1868, 1995
BcgI CGANNNNNNTGC 1 cut(s) 29
BciT130I CCWGG 2 cut(s) 800, 2585
BciVI GTATCC 2 cut(s) 786, 1410
BclI TGATCA 1 cut(s) 1894
BcnI CCSGG 1 cut(s) 2036
BcoDI GTCTC 4 cut(s) 122, 377, 1015, 2117
BfaI CTAG 8 cut(s) 171, 378, 840, 968, 1037, 1563, 1713, 2336
BfmI CTRYAG 4 cut(s) 720, 1500, 1630, 2532
BfuAI ACCTGC 3 cut(s) 789, 2525, 2585
BfuI GTATCC 2 cut(s) 786, 1410
BlpI GCTNAGC 1 cut(s) 273
BmcAI AGTACT 1 cut(s) 1378
Bme1390I CCNGG 3 cut(s) 800, 2036, 2585
Bme18I GGWCC 1 cut(s) 476
BmeRI GACNNNNNGTC 1 cut(s) 390
BmeT110I CYCGRG 1 cut(s) 1193
BmgT120I GGNCC 3 cut(s) 476, 2345, 2514
BmiI GGNNCC 7 cut(s) 477, 478, 1536, 2258, 2411, 2516, 2575
BmrFI CCNGG 3 cut(s) 800, 2036, 2585
BmrI ACTGGG 1 cut(s) 424
BmsI GCATC 9 cut(s) 166, 380, 406, 706, 971, 1523, 1738, 1760, 1925
BmuI ACTGGG 1 cut(s) 424
Bpu1102I GCTNAGC 1 cut(s) 273
BpuEI CTTGAG 3 cut(s) 340, 997, 2031
BpuMI CCSGG 1 cut(s) 2036
BsaI GGTCTC 1 cut(s) 2117
BsaJI CCNNGG 4 cut(s) 123, 1194, 1526, 2035
BsaWI WCCGGW 2 cut(s) 1860, 2311
BsaXI ACNNNNNCTCC 4 cut(s) 564, 594, 1813, 1843
Bsc4I CCNNNNNNNGG 5 cut(s) 435, 498, 1814, 2266, 2318
Bse118I RCCGGY 1 cut(s) 1860
Bse1I ACTGG 4 cut(s) 261, 372, 430, 1538
Bse3DI GCAATG 3 cut(s) 90, 1300, 2548
BseAI TCCGGA 1 cut(s) 2311
BseBI CCWGG 2 cut(s) 800, 2585
BseDI CCNNGG 4 cut(s) 123, 1194, 1526, 2035
BseGI GGATG 3 cut(s) 1277, 1536, 1751
BseLI CCNNNNNNNGG 5 cut(s) 435, 498, 1814, 2266, 2318
BseMI GCAATG 3 cut(s) 90, 1300, 2548
BseMII CTCAG 1 cut(s) 966
BseNI ACTGG 4 cut(s) 261, 372, 430, 1538
BseRI GAGGAG 3 cut(s) 1180, 2330, 2472
BseSI GKGCMC 1 cut(s) 2017
BsgI GTGCAG 2 cut(s) 138, 921
Bsh1236I CGCG 1 cut(s) 1291
BshFI GGCC 7 cut(s) 546, 758, 1099, 1366, 1825, 2346, 2515
BshNI GGYRCC 1 cut(s) 2573
BshTI ACCGGT 1 cut(s) 1860
BsiHKAI GWGCWC 5 cut(s) 508, 1307, 1860, 2017, 2391
BsiHKCI CYCGRG 1 cut(s) 1193
BsiSI CCGG 3 cut(s) 1861, 2035, 2312
BslFI GGGAC 3 cut(s) 462, 924, 1176
BslI CCNNNNNNNGG 5 cut(s) 435, 498, 1814, 2266, 2318
BsmAI GTCTC 4 cut(s) 122, 377, 1015, 2117
BsmBI CGTCTC 1 cut(s) 122
BsmFI GGGAC 3 cut(s) 462, 924, 1176
BsmI GAATGC 1 cut(s) 2566
BsnI GGCC 7 cut(s) 546, 758, 1099, 1366, 1825, 2346, 2515
Bso31I GGTCTC 1 cut(s) 2117
BsoBI CYCGRG 1 cut(s) 1193
Bsp1286I GDGCHC 5 cut(s) 508, 1307, 1860, 2017, 2391
Bsp13I TCCGGA 1 cut(s) 2311
Bsp1407I TGTACA 2 cut(s) 2235, 2614
Bsp143I GATC 9 cut(s) 94, 598, 1321, 1534, 1595, 1765, 1894, 2256, 2372
Bsp1720I GCTNAGC 1 cut(s) 273
Bsp19I CCATGG 1 cut(s) 1526
BspANI GGCC 7 cut(s) 546, 758, 1099, 1366, 1825, 2346, 2515
BspCNI CTCAG 1 cut(s) 967
BspEI TCCGGA 1 cut(s) 2311
BspFNI CGCG 1 cut(s) 1291
BspHI TCATGA 1 cut(s) 1318
BspLI GGNNCC 7 cut(s) 477, 478, 1536, 2258, 2411, 2516, 2575
BspMAI CTGCAG 1 cut(s) 2536
BspMI ACCTGC 3 cut(s) 789, 2525, 2585
BspPI GGATC 7 cut(s) 1316, 1529, 1542, 1603, 2251, 2264, 2380
BspT107I GGYRCC 1 cut(s) 2573
BspTNI GGTCTC 1 cut(s) 2117
BsrDI GCAATG 3 cut(s) 90, 1300, 2548
BsrFI RCCGGY 1 cut(s) 1860
BsrGI TGTACA 2 cut(s) 2235, 2614
BsrI ACTGG 4 cut(s) 261, 372, 430, 1538
BssAI RCCGGY 1 cut(s) 1860
BssECI CCNNGG 4 cut(s) 123, 1194, 1526, 2035
BssMI GATC 9 cut(s) 94, 598, 1321, 1534, 1595, 1765, 1894, 2256, 2372
BssSI CACGAG 2 cut(s) 1586, 1827
BssT1I CCWWGG 2 cut(s) 123, 1526
Bst2BI CACGAG 2 cut(s) 1586, 1827
Bst2UI CCWGG 2 cut(s) 800, 2585
Bst4CI ACNGT 6 cut(s) 752, 1309, 1381, 1741, 1933, 2240
Bst6I CTCTTC 2 cut(s) 1486, 1789
BstAPI GCANNNNNTGC 4 cut(s) 287, 425, 531, 891
BstAUI TGTACA 2 cut(s) 2235, 2614
BstC8I GCNNGC 6 cut(s) 279, 426, 705, 883, 960, 1449
BstDEI CTNAG 5 cut(s) 273, 614, 975, 1263, 2186
BstDSI CCRYGG 1 cut(s) 1526
BstEII GGTNACC 1 cut(s) 776
BstF5I GGATG 3 cut(s) 1277, 1536, 1751
BstFNI CGCG 1 cut(s) 1291
BstHHI GCGC 3 cut(s) 786, 1293, 2045
BstKTI GATC 9 cut(s) 97, 601, 1324, 1537, 1598, 1768, 1897, 2259, 2375
BstMAI GTCTC 4 cut(s) 122, 377, 1015, 2117
BstMBI GATC 9 cut(s) 94, 598, 1321, 1534, 1595, 1765, 1894, 2256, 2372
BstNI CCWGG 2 cut(s) 800, 2585
BstNSI RCATGY 2 cut(s) 1063, 2067
BstPI GGTNACC 1 cut(s) 776
BstSCI CCNGG 3 cut(s) 798, 2034, 2583
BstSFI CTRYAG 4 cut(s) 720, 1500, 1630, 2532
BstSLI GKGCMC 1 cut(s) 2017
BstUI CGCG 1 cut(s) 1291
BstX2I RGATCY 3 cut(s) 1534, 2256, 2372
BstXI CCANNNNNNTGG 1 cut(s) 2252
BstYI RGATCY 3 cut(s) 1534, 2256, 2372
BsuI GTATCC 2 cut(s) 786, 1410
BsuRI GGCC 7 cut(s) 546, 758, 1099, 1366, 1825, 2346, 2515
BtgI CCRYGG 1 cut(s) 1526
BtsCI GGATG 3 cut(s) 1277, 1536, 1751
BtsI GCAGTG 3 cut(s) 892, 1458, 1618
BtsIMutI CAGTG 4 cut(s) 892, 1458, 1618, 1929
BveI ACCTGC 3 cut(s) 789, 2525, 2585
Cac8I GCNNGC 6 cut(s) 279, 426, 705, 883, 960, 1449
CaiI CAGNNNCTG 2 cut(s) 728, 1814
CciI TCATGA 1 cut(s) 1318
CfoI GCGC 3 cut(s) 786, 1293, 2045
Cfr10I RCCGGY 1 cut(s) 1860
Cfr13I GGNCC 3 cut(s) 476, 2345, 2514
CsiI ACCWGGT 1 cut(s) 2583
Csp6I GTAC 8 cut(s) 1087, 1377, 1426, 1640, 2236, 2241, 2588, 2615
CspAI ACCGGT 1 cut(s) 1860
CviQI GTAC 8 cut(s) 1087, 1377, 1426, 1640, 2236, 2241, 2588, 2615
DdeI CTNAG 5 cut(s) 273, 614, 975, 1263, 2186
DpnI GATC 9 cut(s) 96, 600, 1323, 1536, 1597, 1767, 1896, 2258, 2374
DpnII GATC 9 cut(s) 94, 598, 1321, 1534, 1595, 1765, 1894, 2256, 2372
DriI GACNNNNNGTC 1 cut(s) 390
EaeI YGGCCR 3 cut(s) 544, 756, 1364
Eam1104I CTCTTC 2 cut(s) 1486, 1789
Eam1105I GACNNNNNGTC 1 cut(s) 390
EarI CTCTTC 2 cut(s) 1486, 1789
Eco130I CCWWGG 2 cut(s) 123, 1526
Eco147I AGGCCT 1 cut(s) 1825
Eco31I GGTCTC 1 cut(s) 2117
Eco32I GATATC 2 cut(s) 520, 2152
Eco47I GGWCC 1 cut(s) 476
Eco57I CTGAAG 3 cut(s) 38, 471, 497
Eco88I CYCGRG 1 cut(s) 1193
Eco91I GGTNACC 1 cut(s) 776
EcoO109I RGGNCCY 3 cut(s) 476, 2345, 2514
EcoO65I GGTNACC 1 cut(s) 776
EcoRI GAATTC 2 cut(s) 1156, 2281
EcoRII CCWGG 2 cut(s) 798, 2583
EcoRV GATATC 2 cut(s) 520, 2152
EcoT14I CCWWGG 2 cut(s) 123, 1526
EcoT22I ATGCAT 2 cut(s) 745, 1753
ErhI CCWWGG 2 cut(s) 123, 1526
Esp3I CGTCTC 1 cut(s) 122
FalI AAGNNNNNCTT 6 cut(s) 669, 701, 1975, 2007, 2538, 2570
FaqI GGGAC 3 cut(s) 462, 924, 1176
FauNDI CATATG 3 cut(s) 73, 1998, 2082
FbaI TGATCA 1 cut(s) 1894
FblI GTMKAC 1 cut(s) 952
FokI GGATG 3 cut(s) 1284, 1543, 1738
FspBI CTAG 8 cut(s) 171, 378, 840, 968, 1037, 1563, 1713, 2336
FspI TGCGCA 1 cut(s) 785
GlaI GCGC 3 cut(s) 785, 1292, 2044
HaeIII GGCC 7 cut(s) 546, 758, 1099, 1366, 1825, 2346, 2515
HapII CCGG 3 cut(s) 1861, 2035, 2312
HhaI GCGC 3 cut(s) 786, 1293, 2045
Hin6I GCGC 3 cut(s) 784, 1291, 2043
HinP1I GCGC 3 cut(s) 784, 1291, 2043
HincII GTYRAC 1 cut(s) 1312
HindII GTYRAC 1 cut(s) 1312
HindIII AAGCTT 1 cut(s) 1282
HinfI GANTC 6 cut(s) 908, 1478, 1811, 1925, 1946, 2288
HpaII CCGG 3 cut(s) 1861, 2035, 2312
HphI GGTGA 1 cut(s) 1268
Hpy166II GTNNAC 5 cut(s) 307, 953, 1312, 2015, 2615
Hpy188I TCNGA 9 cut(s) 94, 594, 976, 1057, 1336, 1477, 1921, 2226, 2287
Hpy8I GTNNAC 5 cut(s) 307, 953, 1312, 2015, 2615
HpyAV CCTTC 7 cut(s) 521, 849, 953, 973, 2155, 2168, 2486
HpyCH4III ACNGT 6 cut(s) 752, 1309, 1381, 1741, 1933, 2240
HpyCH4IV ACGT 1 cut(s) 131
HpyF3I CTNAG 5 cut(s) 273, 614, 975, 1263, 2186
HpySE526I ACGT 1 cut(s) 131
HspAI GCGC 3 cut(s) 784, 1291, 2043
KflI GGGWCCC 1 cut(s) 476
Kpn2I TCCGGA 1 cut(s) 2311
Ksp22I TGATCA 1 cut(s) 1894
Kzo9I GATC 9 cut(s) 94, 598, 1321, 1534, 1595, 1765, 1894, 2256, 2372
LmnI GCTCC 6 cut(s) 402, 931, 1167, 1474, 2037, 2060
LweI GCATC 9 cut(s) 166, 380, 406, 706, 971, 1523, 1738, 1760, 1925
MabI ACCWGGT 1 cut(s) 2583
MaeI CTAG 8 cut(s) 171, 378, 840, 968, 1037, 1563, 1713, 2336
MaeII ACGT 1 cut(s) 131
MaeIII GTNAC 5 cut(s) 137, 570, 776, 2338, 2624
MalI GATC 9 cut(s) 96, 600, 1323, 1536, 1597, 1767, 1896, 2258, 2374
MboI GATC 9 cut(s) 94, 598, 1321, 1534, 1595, 1765, 1894, 2256, 2372
MboII GAAGA 9 cut(s) 689, 938, 1473, 1757, 1776, 2170, 2322, 2367, 2657
MfeI CAATTG 1 cut(s) 105
MflI RGATCY 3 cut(s) 1534, 2256, 2372
MhlI GDGCHC 5 cut(s) 508, 1307, 1860, 2017, 2391
MlsI TGGCCA 3 cut(s) 546, 758, 1366
MluNI TGGCCA 3 cut(s) 546, 758, 1366
MlyI GAGTC 1 cut(s) 1472
MmeI TCCRAC 4 cut(s) 1044, 1799, 1938, 2038
Mox20I TGGCCA 3 cut(s) 546, 758, 1366
Mph1103I ATGCAT 2 cut(s) 745, 1753
MroI TCCGGA 1 cut(s) 2311
MroXI GAANNNNTTC 3 cut(s) 456, 1388, 2281
MscI TGGCCA 3 cut(s) 546, 758, 1366
MseI TTAA 2 cut(s) 347, 2662
MslI CAYNNNNRTG 1 cut(s) 829
Msp20I TGGCCA 3 cut(s) 546, 758, 1366
MspI CCGG 3 cut(s) 1861, 2035, 2312
MspR9I CCNGG 3 cut(s) 800, 2036, 2585
MunI CAATTG 1 cut(s) 105
Mva1269I GAATGC 1 cut(s) 2566
MvaI CCWGG 2 cut(s) 800, 2585
MvnI CGCG 1 cut(s) 1291
NciI CCSGG 1 cut(s) 2036
NcoI CCATGG 1 cut(s) 1526
NdeI CATATG 3 cut(s) 73, 1998, 2082
NdeII GATC 9 cut(s) 94, 598, 1321, 1534, 1595, 1765, 1894, 2256, 2372
NlaIV GGNNCC 7 cut(s) 477, 478, 1536, 2258, 2411, 2516, 2575
NmuCI GTSAC 1 cut(s) 137
NsbI TGCGCA 1 cut(s) 785
NsiI ATGCAT 2 cut(s) 745, 1753
NspI RCATGY 2 cut(s) 1063, 2067
PagI TCATGA 1 cut(s) 1318
PaqCI CACCTGC 1 cut(s) 2585
PceI AGGCCT 1 cut(s) 1825
PciI ACATGT 1 cut(s) 1059
PctI GAATGC 1 cut(s) 2566
PdmI GAANNNNTTC 3 cut(s) 456, 1388, 2281
PfeI GAWTC 5 cut(s) 908, 1811, 1925, 1946, 2288
PflMI CCANNNNNTGG 1 cut(s) 1814
PfoI TCCNGGA 1 cut(s) 798
PinAI ACCGGT 1 cut(s) 1860
PleI GAGTC 1 cut(s) 1472
PpsI GAGTC 1 cut(s) 1472
PpuMI RGGWCCY 1 cut(s) 476
PscI ACATGT 1 cut(s) 1059
Psp5II RGGWCCY 1 cut(s) 476
Psp6I CCWGG 2 cut(s) 798, 2583
PspEI GGTNACC 1 cut(s) 776
PspGI CCWGG 2 cut(s) 798, 2583
PspN4I GGNNCC 7 cut(s) 477, 478, 1536, 2258, 2411, 2516, 2575
PspPI GGNCC 3 cut(s) 476, 2345, 2514
PspPPI RGGWCCY 1 cut(s) 476
PstI CTGCAG 1 cut(s) 2536
PstNI CAGNNNCTG 2 cut(s) 728, 1814
PsuI RGATCY 3 cut(s) 1534, 2256, 2372
RsaI GTAC 8 cut(s) 1088, 1378, 1427, 1641, 2237, 2242, 2589, 2616
RsaNI GTAC 8 cut(s) 1087, 1377, 1426, 1640, 2236, 2241, 2588, 2615
RseI CAYNNNNRTG 1 cut(s) 829
SaqAI TTAA 2 cut(s) 347, 2662
Sau3AI GATC 9 cut(s) 94, 598, 1321, 1534, 1595, 1765, 1894, 2256, 2372
Sau96I GGNCC 3 cut(s) 476, 2345, 2514
ScaI AGTACT 1 cut(s) 1378
SchI GAGTC 1 cut(s) 1472
ScrFI CCNGG 3 cut(s) 800, 2036, 2585
SduI GDGCHC 5 cut(s) 508, 1307, 1860, 2017, 2391
SexAI ACCWGGT 1 cut(s) 2583
SfaNI GCATC 9 cut(s) 166, 380, 406, 706, 971, 1523, 1738, 1760, 1925
SfcI CTRYAG 4 cut(s) 720, 1500, 1630, 2532
SinI GGWCC 1 cut(s) 476
SmiMI CAYNNNNRTG 1 cut(s) 829
SmlI CTYRAG 3 cut(s) 319, 1012, 2046
SmoI CTYRAG 3 cut(s) 319, 1012, 2046
SseBI AGGCCT 1 cut(s) 1825
SspI AATATT 1 cut(s) 2599
SspMI CTAG 8 cut(s) 171, 378, 840, 968, 1037, 1563, 1713, 2336
StuI AGGCCT 1 cut(s) 1825
StyD4I CCNGG 3 cut(s) 798, 2034, 2583
StyI CCWWGG 2 cut(s) 123, 1526
TaaI ACNGT 6 cut(s) 752, 1309, 1381, 1741, 1933, 2240
TaiI ACGT 1 cut(s) 134
TaqI TCGA 5 cut(s) 157, 601, 1160, 1301, 1949
TatI WGTACW 5 cut(s) 1376, 1425, 1639, 2235, 2614
TfiI GAWTC 5 cut(s) 908, 1811, 1925, 1946, 2288
Tru1I TTAA 2 cut(s) 347, 2662
Tru9I TTAA 2 cut(s) 347, 2662
TscAI CASTG 4 cut(s) 892, 1458, 1618, 1936
TseFI GTSAC 1 cut(s) 137
Tsp45I GTSAC 1 cut(s) 137
TspRI CASTG 4 cut(s) 892, 1458, 1618, 1936
Van91I CCANNNNNTGG 1 cut(s) 1814
VneI GTGCAC 1 cut(s) 2013
VpaK11BI GGWCC 1 cut(s) 476
XapI RAATTY 5 cut(s) 183, 1156, 1691, 2197, 2281
XbaI TCTAGA 1 cut(s) 1036
XceI RCATGY 2 cut(s) 1063, 2067
XcmI CCANNNNNNNNNTGG 1 cut(s) 1815
XmiI GTMKAC 1 cut(s) 952
XmnI GAANNNNTTC 3 cut(s) 456, 1388, 2281
XspI CTAG 8 cut(s) 171, 378, 840, 968, 1037, 1563, 1713, 2336
ZrmI AGTACT 1 cut(s) 1378
Zsp2I ATGCAT 2 cut(s) 745, 1753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.