MD07G1058300.v1.1

Phosphoinositide 3-kinase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001323
Physical Location & Seq
Forward (+)
5412869 .. 5420849
7981 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1058300.v1.1.491

Sequence Viewer

Length: 4665 bp
ATGGGGAACAAGATCGCTCGAACGACGCAAGTGTCGGCGTCCGAGTACTACCTCCACGACCTGCCGTCGTCCTACAACCTGGTCCTGAAGGAGGTTCTAGGGCGGAGCCGCTTCTTCAAGTCGATTGAGTGCAAGCACGACGAGGGTTTGGTCCTCGTCAAGGTCTATTTCAAGCGCGGCGACTCCATTGATCTCCGTGAATACGAGCGCCGTCTTTACCACATCAAGGACACCTTCCGTGCTCTCGATCACCCTCACGTTTGGCCCTTCCAGTTTTGGCAAGAGACAGATAAAGCAGCTTATCTTGTGAGGCAGTTCTTCTTCAACAATCTGCATGACCGATTAAGCACACGCCCTTTTCTCAGTCTAATCGAGAAGAAATGGTTGGCTTTTCAGTTACTTCTTGCTCTGAAGCAGTGCCACGACAAAGGAATATGTCACGGTGATATTAAGTGCGAGAATGTATTGGTGACTTCTTGGAATTGGCTTTACCTTGCTGACTTCGCATCTTTCAAACCCACCTACATACCATATGACGATCCTTCTGATTTCTCTTTTTTCTTTGACACTGGAGGAAGAAGGCTTTGTTATCTTGCTCCCGAGAGATTTTATGAGCATGGAGGTGAGATGCAGGTTGCACAAGATGCACCATTAAGGCCATCTATGGACATATTTGCTGTAGGGTGTGTAATTGCTGAGCTTTTCCTTGAGGGCCAGCCACTATTCGAACTCTCTCAACTTCTTGCTTATCGTAGAGGGCAGTATGATCCTAGCCAACTTCTTGAAAAGATACCTGATTCTGGGATTCGCAAGATGATACTTCATATGATTCAGTTGGAACCAGAGCTGCGACTTTCTGCTGATAGCTACCTACAGGAGTATACAACTGTTGTGTTTCCAAGTTACTTCTCTCCGTTTCTGCATAATTTTCATTGTTTCTGGAATCCCCTTCATTCAGATATGAGGGTTGCACTTTGCCAGAGTGTTTTTCATGAGATACTTAAACAAATGATGAGCAGTAGGTCAACTGAGGACAGTGGAAACGGACTGGGAGCTCCTTCAAGTGCCAATGCCATTAGTGGTAGAACTTCTCAGGAGGCGGTTGGAATGCAGGACAAGAGTTTGACAAAGGGCTTGTTTCCAAAGAGAGAAGAGATGGGTAAAGGCTTAAAATGTGATCAATTAGAACTTCTTGGTGATATCAATACTCTTCTTCGGGATGTTAAACAAAGTAATAATTACTCTGCTACCAAGGCAATGCTGAATGATATTCCTGATTCTGCGTTTTCCCCGAATCTTGGAAACTATGGCATGCAATCTCCTGGTGATTTACTTCAATCTATCTCCAATGCATTTAGGAGAAACGATCATCCTTTTCTGAAAAAGATTACATTGAATGATTTGAATTCATTGATGTCCAAGTATGACAGCCAGTCAGATACCTTTGGCATGCCGTTTCTGCCTTTACCTGAAGACAGTATCAGATGTGAAGGCATGGTTCTGATTACCTCACTGCTCTGTTCCTGCATACGTAATGTAAAATTGCCTCATTTAAGGAGGAGGGCCATACTCTTGCTGAAATCGTCTGCCTTGTACATTGATGATGAAGATCGTTTGCAACGTGTAATTCCATATGTTGTTGCAATGCTTTCAGATTCAGCTGCAATTGTGCGTTGTGCTGCCTTGGAGACGTTGTGTGACATTCTCCCTCTAGTCCAGGATTTTCCTCCCAGTGATGCAAAAATTTTTCCGGAGTATATATTGCCAATGCTTTCCATGCTTCCTGATGACCCAGAGGAAAGCGTGAGAATATGTTATGCCAGTAATATAGCTAAGCTGGCATTAACTGCTTATGGTTTCTTGGTTCACTCAATACGCTTGAGTGAGGCTGGGGTACTTGATGAATTAACTTCTGCAAAAAAGCCGCTGGTATCATCTAGTGAGACATCTGGTCAACTGCAGAGGGTGAATAGCGATGCACAGCTTGCAGTGTTGAGGAAATCTATAGCTGAGGTCATTCAAGAACTTGTTATGGGTCCAAAGCAAACTCCAAATATCAGGAGAGCACTCCTTCAGGACATTAGCAACCTATGCTGCTTCTTTGGCCAGAGACAGAGTAATGATTTTTTGTTACCCATTCTACCTGCTTTTCTGAATGATCGAGATGAGCAGCTGAGAGCAGTATTCTATGGGCAAATTGTATATGTGTGTTTCTTTGTGGGTCAAAGAAGTGTGGAAGAATATCTTTTACCTTATATTGAGCAGGCTGTAAGTGATGTAACAGAGGCTGTCATTGTCAATGCATTGGACTGTCTGGCCATTTTGTGCAAAAGTGGTTTCTTGACGAAGAGGATACTTCTAGAAATGATTGAGCGTGCATTTCCATTGTTATGCTATCCCAGTCAGTGGGTAAGAAGGTCAGCTGTCACTTTTATTGCAGCCAGTAGTGAAAGCTTAGGTGCTGTAGATTCCTATGTCTTTCTAGCCCCTGTTATTCGGCCCTTACTCCGCAGACAGCCAGCATCTCTTGCTTCTGAAAAGGCTCTCCTTTCGTCTTTAAAACCTCCAGTCTCAAGACAGGTTTTTTACCAAGTTCTAGAAAATGCAAGGAGTTCAGACATGTTGGAAAGACAGAGAAAGATATGGTACAACTCTTGGCCTCAGTCTAAACAATGGGAAAGTGTTGACTTGCTTCAGCAAGGGGTTGAGGAACTGAGTTCAACAAAAAGCTGGCCAGCCGAGCACCAGAGTCCTGAAAATCATAAACTTACCGGAAAGGCCTTGCAACAAGGAGAGTTAACTGAATGTGATGACGGTGAGGCTAAGTTGAGAAGTATGGGAAGCTTTACGCCCGCTTCTAGCACAGTTGACATTCATGATCCTTTGTCCTCTGAAAAAATGCAATTTTCAGGTTTTATGTGGCCACAGGGAAGTTATGTGAATAGCTTCATGTGTGATAAATCATCAGTTGGCATACCTTTATACTCCTTTAGCATGGACAAGCGAGCAGTGGGAGTTCCTTCTCCAGCATCTGATTCTCCTTCACAAGTGAATCCCGTAGGAATTGGTGCATCAGCCATGCCTTGGATGGAGCCAGTAAATAAATCATTTAGTTTGGCTAGTTCTGTTCCAGCACCTAAGCTTGTGTCAGGATCATTCAATTTTGGCAATGGTTCCAAACAGTTCTATAGAGTTGTACATGAGCCAGATGGTAGGGATAATGATCAAACCACCTTTGCTAATAGCAAGCTTCAAAATATGGGATTATCTGGCACTACAAAAGGAAGTGCCATACCCTTGGAAGATGCATCCACTTCAAGTGATCTAACAGGAATGCCGTCTTCTGCACGAAATTCATCAATTCCAGACTCTGGTTGGAGGCCTCGTGGGGTGTTGGTTGCTCACCTGCAAGAACATCGGTCTGCTGTCAATGACATAGCCATTTCAACTGATCATAGCTTTTTTGTGAGCGCATCTGATGATTCCACTGTTAAGGTGTGGGATTCAAGAAAGTTGGAAAAGGACATATCATTTCGGTCAAGGTTGACTTATCATCTAGAGGGAAGCCGTGCACTTTGCAGTGCAATGCTCCGGGGGTCTGCTCGAGTCGTTGTAGGAGCATGTGATGGCATGATACATATGTTTTCTGTTGATTACATCTCCAGGGGACTTGGTAATGTGGTTGAAAAGTATTCAGGTGTTGCTGATATCAAGAAGAAGGATATAAAGGAAGGTGCCATATTGAGCTTGTTGAATTTCTCAGCGGATAACTGCACAAATCAGATGGTTATGTACAGCACCCAAAATTGTGGTATCCATCTTTGGGATACAAGGTTGAACACCAATTCTTGGACATTGAGAGCAACTCCTGAGGAGGGCTACGTGTCTTCGCTGGTAACAGGCTCCTGTGAAAATTGGTTTGTGTCAGGATCTTCGAGGGGTGTGCTTACTCTTTGGGATATGAGGTTCCTTATTCCTGTAAACTCATGGCAGTATTCTTCTGTTTGCCCTATAGAGAAAATGTGCCTTTTTCTTCCTCCACCTAATGCCTCTGTGTCTGCTGCTGCAAGGCCCCTTATTTATGTTGCTGCAGGTTGCAACGAAGTTTCTCTTTGGAATGCTGAAAATGGAAGCTGCCACCAGGTGCTGAGAGTGGCAAGTTACGAAAGTGATGCTGAAATGTCTGAAGTGCCTTGGGCCTTGGCTAAGTCATCAAGTAAAAGTTCCAAACCAGATATGAGACGGAATGGCAATCCCCACTACAGAGTTGATGAACTAAATGAACCCACTCCTCGTCTTCCTGGCATCCGTTCATTGCTACCCTTACCTGGGGGTGATTTGATAACTGGAGGAACCGATTTGAAGATACGTCGATGGGATCATTACAGCCCCGATCGAAGTTACTCAATATGTGGGCCAAACTTGAAGGGAGTCGGTAATGATGATTTCTATGCAACAAGATCTAGCTTTGGTGTGCAAGTTGTGCAGGAGACAAAAAGACGACCTCTAACATCAAAGCTGACAGCCAAGGCAGTCCTTGCGGCTGCTGCCACTGATTCTGCTGGCTGCCACCGCGATTCAATCCTATCCTTGGCCTCGGTTAAGTTAAATCAGAGACACTTAATATCAAGTGGTAGAGATGGGGCAATTAAGGTTTGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000011 GO:0000151 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005737 GO:0005768 GO:0005770 GO:0005942 GO:0006355 GO:0006357 GO:0006464 GO:0006468 GO:0006605 GO:0006623 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006886 GO:0006914 GO:0006996 GO:0007033 GO:0007034 GO:0007154 GO:0007165 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009555 GO:0009846 GO:0009856 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010008 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0016236 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019219 GO:0019222 GO:0019538 GO:0019898 GO:0022414 GO:0023052 GO:0030242 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031410 GO:0031461 GO:0031967 GO:0031975 GO:0031982 GO:0032182 GO:0032501 GO:0032502 GO:0032507 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0033036 GO:0033365 GO:0034067 GO:0034243 GO:0034271 GO:0034272 GO:0034613 GO:0035032 GO:0035556 GO:0036211 GO:0042886 GO:0043130 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0044706 GO:0045053 GO:0045184 GO:0045185 GO:0045324 GO:0045893 GO:0045935 GO:0045944 GO:0046907 GO:0048015 GO:0048017 GO:0048229 GO:0048308 GO:0048518 GO:0048522 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051235 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051651 GO:0051704 GO:0051716 GO:0060255 GO:0061695 GO:0061919 GO:0065007 GO:0065008 GO:0070727 GO:0071561 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0080008 GO:0080090 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0120095 GO:0140096 GO:1901564 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04136 ko04138 ko04140 ko04371 map04136 map04138 map04140 map04371
Pfam Domains
Protein Families
HEAT Pkinase WD40

Protein Analysis

1555

Amino Acids

173.49

Weight (kDa)

6.64

Isoelectric Point (pI)

53.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 30 - 292 1.2e-19 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 30 - 240 5.7e-06 Protein tyrosine and serine/threonine kinase
VPS15-like_hel PF22956 502 - 865 1.5e-123 VPS15-like, helical domain
Beta-prop_WDR5 PF25175 1130 - 1217 1.1e-08 WDR5 beta-propeller domain
WD40_Prp19 PF24814 1130 - 1335 2e-08 Prp19 WD40 domain
WD40 PF00400 1131 - 1168 1e-08 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 1133 - 1216 9.8e-06 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 1134 - 1329 4.3e-08 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 1141 - 1342 2.5e-06 WDHD1 first WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3414
AasI GACNNNNNNGTC 1 cut(s) 31
Acc36I ACCTGC 5 cut(s) 69, 622, 2152, 3414, 4082
AccB1I GGYRCC 1 cut(s) 3734
AccB7I CCANNNNNTGG 4 cut(s) 2406, 3084, 3371, 3849
AccI GTMKAC 1 cut(s) 881
AccII CGCG 2 cut(s) 177, 4575
AccIII TCCGGA 1 cut(s) 1750
AclWI GGATC 6 cut(s) 533, 761, 2873, 3160, 3937, 4386
AcoI YGGCCR 4 cut(s) 2104, 2316, 2732, 2921
AcsI RAATTY 4 cut(s) 1405, 1743, 3352, 3754
AcuI CTGAAG 6 cut(s) 107, 431, 1491, 2057, 2678, 4206
AcyI GRCGYC 1 cut(s) 38
AdeI CACNNNGTG 1 cut(s) 4144
AfaI GTAC 6 cut(s) 47, 1595, 1896, 2648, 3198, 3794
AflIII ACRYGT 3 cut(s) 1621, 2619, 3882
AhdI GACNNNNNGTC 3 cut(s) 64, 1432, 1950
AjnI CCWGG 7 cut(s) 78, 1321, 1716, 3662, 4140, 4300, 4327
AjuI GAANNNNNNNTTGG 2 cut(s) 368, 400
AloI GAACNNNNNNTCC 2 cut(s) 3950, 3982
Alw21I GWGCWC 5 cut(s) 244, 1057, 2068, 2745, 3574
Alw26I GTCTC 8 cut(s) 278, 1682, 1937, 2104, 2575, 4234, 4484, 4609
Alw44I GTGCAC 1 cut(s) 3570
AlwI GGATC 6 cut(s) 533, 761, 2873, 3160, 3937, 4386
AlwNI CAGNNNCTG 4 cut(s) 2288, 3032, 3371, 4147
Ama87I CYCGRG 2 cut(s) 599, 3603
Aor13HI TCCGGA 1 cut(s) 1750
ApaLI GTGCAC 1 cut(s) 3570
ApoI RAATTY 4 cut(s) 1405, 1743, 3352, 3754
Asp700I GAANNNNTTC 1 cut(s) 2945
AspLEI GCGC 3 cut(s) 177, 210, 3473
AsuC2I CCSGG 1 cut(s) 3593
AsuII TTCGAA 1 cut(s) 726
AvaI CYCGRG 2 cut(s) 599, 3603
AvaII GGWCC 3 cut(s) 82, 151, 2036
AxyI CCTNAGG 1 cut(s) 3870
BaeGI GKGCMC 1 cut(s) 3574
BaeI ACNNNNGTAYC 2 cut(s) 3626, 3659
BalI TGGCCA 4 cut(s) 2106, 2318, 2734, 2923
BanI GGYRCC 1 cut(s) 3734
BanII GRGCYC 1 cut(s) 1057
BauI CACGAG 1 cut(s) 3384
BbsI GAAGAC 4 cut(s) 1479, 3333, 3879, 4289
Bbv12I GWGCWC 5 cut(s) 244, 1057, 2068, 2745, 3574
BbvCI CCTCAGC 1 cut(s) 2010
BccI CCATC 9 cut(s) 667, 1150, 3082, 3203, 3620, 3778, 3825, 4368, 4634
BceAI ACGGC 5 cut(s) 49, 195, 1438, 3322, 3552
BcgI CGANNNNNNTGC 6 cut(s) 3398, 3432, 3925, 3959, 4154, 4188
BciT130I CCWGG 7 cut(s) 80, 1323, 1718, 3664, 4142, 4302, 4329
BciVI GTATCC 3 cut(s) 2346, 3820, 3824
BclI TGATCA 3 cut(s) 1177, 3223, 3451
BcnI CCSGG 1 cut(s) 3593
BcoDI GTCTC 8 cut(s) 278, 1682, 1937, 2104, 2575, 4234, 4484, 4609
BfmI CTRYAG 9 cut(s) 678, 872, 1958, 2004, 2463, 3187, 4011, 4089, 4261
BfoI RGCGCY 1 cut(s) 211
BfuAI ACCTGC 5 cut(s) 69, 622, 2152, 3414, 4082
BfuI GTATCC 3 cut(s) 2346, 3820, 3824
BglII AGATCT 1 cut(s) 4460
BlpI GCTNAGC 2 cut(s) 696, 1833
BmcAI AGTACT 1 cut(s) 47
Bme1390I CCNGG 8 cut(s) 80, 1323, 1718, 3593, 3664, 4142, 4302, 4329
Bme18I GGWCC 3 cut(s) 82, 151, 2036
BmeRI GACNNNNNGTC 3 cut(s) 64, 1432, 1950
BmeT110I CYCGRG 2 cut(s) 599, 3603
BmrFI CCNGG 8 cut(s) 80, 1323, 1718, 3593, 3664, 4142, 4302, 4329
BmrI ACTGGG 3 cut(s) 1058, 1725, 2394
BmuI ACTGGG 3 cut(s) 1058, 1725, 2394
BpiI GAAGAC 4 cut(s) 1479, 3333, 3879, 4289
BplI GAGNNNNNCTC 2 cut(s) 3850, 3882
BpmI CTGGAG 5 cut(s) 591, 2550, 3009, 3646, 4368
Bpu10I CCTNAGC 3 cut(s) 2010, 2455, 3138
Bpu1102I GCTNAGC 2 cut(s) 696, 1833
Bpu14I TTCGAA 1 cut(s) 726
BpuEI CTTGAG 3 cut(s) 728, 1900, 2557
BpuMI CCSGG 1 cut(s) 3593
BsaAI YACGTR 2 cut(s) 1532, 3883
BsaBI GATNNNNATC 2 cut(s) 1608, 3222
BsaHI GRCGYC 1 cut(s) 38
BsaWI WCCGGW 2 cut(s) 1750, 2771
BsaXI ACNNNNNCTCC 4 cut(s) 1679, 1709, 3370, 3400
Bse21I CCTNAGG 1 cut(s) 3870
Bse3DI GCAATG 5 cut(s) 1263, 1650, 3175, 3591, 4313
Bse8I GATNNNNATC 2 cut(s) 1608, 3222
BseAI TCCGGA 1 cut(s) 1750
BseBI CCWGG 7 cut(s) 80, 1323, 1718, 3664, 4142, 4302, 4329
BseGI GGATG 5 cut(s) 1225, 1369, 3093, 3308, 4305
BseJI GATNNNNATC 2 cut(s) 1608, 3222
BseMI GCAATG 5 cut(s) 1263, 1650, 3175, 3591, 4313
BseRI GAGGAG 3 cut(s) 1573, 3887, 4281
BseSI GKGCMC 1 cut(s) 3574
BseYI CCCAGC 1 cut(s) 1889
BsgI GTGCAG 3 cut(s) 3330, 3757, 4505
Bsh1236I CGCG 2 cut(s) 177, 4575
Bsh1285I CGRYCG 1 cut(s) 4396
BshNI GGYRCC 1 cut(s) 3734
BsiEI CGRYCG 1 cut(s) 4396
BsiHKAI GWGCWC 5 cut(s) 244, 1057, 2068, 2745, 3574
BsiHKCI CYCGRG 2 cut(s) 599, 3603
BsiSI CCGG 3 cut(s) 1751, 2772, 3592
BslFI GGGAC 1 cut(s) 3681
BsmAI GTCTC 8 cut(s) 278, 1682, 1937, 2104, 2575, 4234, 4484, 4609
BsmBI CGTCTC 2 cut(s) 1682, 4234
BsmFI GGGAC 1 cut(s) 3681
BsmI GAATGC 3 cut(s) 1113, 3339, 4123
BsoBI CYCGRG 2 cut(s) 599, 3603
Bsp119I TTCGAA 1 cut(s) 726
Bsp1286I GDGCHC 5 cut(s) 244, 1057, 2068, 2745, 3574
Bsp13I TCCGGA 1 cut(s) 1750
Bsp1407I TGTACA 3 cut(s) 1593, 3196, 3792
Bsp1720I GCTNAGC 2 cut(s) 696, 1833
BspEI TCCGGA 1 cut(s) 1750
BspFNI CGCG 2 cut(s) 177, 4575
BspHI TCATGA 2 cut(s) 991, 2875
BspMAI CTGCAG 2 cut(s) 1962, 4093
BspMI ACCTGC 5 cut(s) 69, 622, 2152, 3414, 4082
BspPI GGATC 6 cut(s) 533, 761, 2873, 3160, 3937, 4386
BspT104I TTCGAA 1 cut(s) 726
BspT107I GGYRCC 1 cut(s) 3734
BsrDI GCAATG 5 cut(s) 1263, 1650, 3175, 3591, 4313
BsrGI TGTACA 3 cut(s) 1593, 3196, 3792
BssNAI GTATAC 1 cut(s) 882
BssNI GRCGYC 1 cut(s) 38
BssSI CACGAG 1 cut(s) 3384
BssT1I CCWWGG 8 cut(s) 1251, 1683, 3083, 3297, 4193, 4200, 4527, 4590
Bst1107I GTATAC 1 cut(s) 882
Bst2BI CACGAG 1 cut(s) 3384
Bst2UI CCWGG 7 cut(s) 80, 1323, 1718, 3664, 4142, 4302, 4329
Bst4CI ACNGT 9 cut(s) 443, 889, 1037, 1478, 2312, 2816, 2866, 3183, 3490
Bst6I CTCTTC 3 cut(s) 1146, 1215, 2342
BstACI GRCGYC 1 cut(s) 38
BstAPI GCANNNNNTGC 7 cut(s) 644, 1847, 1985, 2091, 2528, 4483, 4538
BstAUI TGTACA 3 cut(s) 1593, 3196, 3792
BstBAI YACGTR 2 cut(s) 1532, 3883
BstBI TTCGAA 1 cut(s) 726
BstENI CCTNNNNNAGG 2 cut(s) 89, 158
BstF5I GGATG 5 cut(s) 1225, 1369, 3093, 3308, 4305
BstFNI CGCG 2 cut(s) 177, 4575
BstH2I RGCGCY 1 cut(s) 211
BstHHI GCGC 3 cut(s) 177, 210, 3473
BstMAI GTCTC 8 cut(s) 278, 1682, 1937, 2104, 2575, 4234, 4484, 4609
BstMCI CGRYCG 1 cut(s) 4396
BstNI CCWGG 7 cut(s) 80, 1323, 1718, 3664, 4142, 4302, 4329
BstNSI RCATGY 4 cut(s) 1315, 1453, 2623, 3624
BstSCI CCNGG 8 cut(s) 78, 1321, 1716, 3591, 3662, 4140, 4300, 4327
BstSFI CTRYAG 9 cut(s) 678, 872, 1958, 2004, 2463, 3187, 4011, 4089, 4261
BstSLI GKGCMC 1 cut(s) 3574
BstSNI TACGTA 1 cut(s) 1532
BstUI CGCG 2 cut(s) 177, 4575
BstV2I GAAGAC 4 cut(s) 1479, 3333, 3879, 4289
BstX2I RGATCY 2 cut(s) 3929, 4460
BstXI CCANNNNNNTGG 2 cut(s) 3298, 3809
BstYI RGATCY 2 cut(s) 3929, 4460
BstZ17I GTATAC 1 cut(s) 882
Bsu36I CCTNAGG 1 cut(s) 3870
BsuI GTATCC 3 cut(s) 2346, 3820, 3824
BtgZI GCGATG 1 cut(s) 1989
BtsCI GGATG 5 cut(s) 1225, 1369, 3093, 3308, 4305
BtsI GCAGTG 5 cut(s) 422, 1511, 1995, 3015, 3586
BveI ACCTGC 5 cut(s) 69, 622, 2152, 3414, 4082
CaiI CAGNNNCTG 4 cut(s) 2288, 3032, 3371, 4147
CciI TCATGA 2 cut(s) 991, 2875
CfoI GCGC 3 cut(s) 177, 210, 3473
CseI GACGC 2 cut(s) 27, 34
CsiI ACCWGGT 2 cut(s) 78, 4140
Csp6I GTAC 6 cut(s) 46, 1594, 1895, 2647, 3197, 3793
CviQI GTAC 6 cut(s) 46, 1594, 1895, 2647, 3197, 3793
DraI TTTAAA 1 cut(s) 2559
DraIII CACNNNGTG 1 cut(s) 4144
DrdI GACNNNNNNGTC 1 cut(s) 31
DriI GACNNNNNGTC 3 cut(s) 64, 1432, 1950
DseDI GACNNNNNNGTC 1 cut(s) 31
EaeI YGGCCR 4 cut(s) 2104, 2316, 2732, 2921
Eam1104I CTCTTC 3 cut(s) 1146, 1215, 2342
Eam1105I GACNNNNNGTC 3 cut(s) 64, 1432, 1950
EarI CTCTTC 3 cut(s) 1146, 1215, 2342
EciI GGCGGA 1 cut(s) 118
Ecl136II GAGCTC 1 cut(s) 1055
Eco105I TACGTA 1 cut(s) 1532
Eco130I CCWWGG 8 cut(s) 1251, 1683, 3083, 3297, 4193, 4200, 4527, 4590
Eco147I AGGCCT 2 cut(s) 2780, 3382
Eco24I GRGCYC 1 cut(s) 1057
Eco32I GATATC 2 cut(s) 1201, 3709
Eco47I GGWCC 3 cut(s) 82, 151, 2036
Eco53kI GAGCTC 1 cut(s) 1055
Eco57I CTGAAG 6 cut(s) 107, 431, 1491, 2057, 2678, 4206
Eco81I CCTNAGG 1 cut(s) 3870
Eco88I CYCGRG 2 cut(s) 599, 3603
EcoICRI GAGCTC 1 cut(s) 1055
EcoNI CCTNNNNNAGG 2 cut(s) 89, 158
EcoO109I RGGNCCY 1 cut(s) 4071
EcoRI GAATTC 1 cut(s) 1405
EcoRII CCWGG 7 cut(s) 78, 1321, 1716, 3662, 4140, 4300, 4327
EcoRV GATATC 2 cut(s) 1201, 3709
EcoT14I CCWWGG 8 cut(s) 1251, 1683, 3083, 3297, 4193, 4200, 4527, 4590
EcoT22I ATGCAT 3 cut(s) 1354, 2305, 3310
EcoT38I GRGCYC 1 cut(s) 1057
ErhI CCWWGG 8 cut(s) 1251, 1683, 3083, 3297, 4193, 4200, 4527, 4590
Esp3I CGTCTC 2 cut(s) 1682, 4234
FalI AAGNNNNNCTT 8 cut(s) 218, 250, 2229, 2261, 3532, 3564, 4095, 4127
FaqI GGGAC 1 cut(s) 3681
FauI CCCGC 1 cut(s) 2860
FauNDI CATATG 4 cut(s) 532, 825, 1633, 3639
FbaI TGATCA 3 cut(s) 1177, 3223, 3451
FblI GTMKAC 1 cut(s) 881
FokI GGATG 5 cut(s) 1232, 1356, 3100, 3295, 4292
FriOI GRGCYC 1 cut(s) 1057
GlaI GCGC 3 cut(s) 176, 209, 3472
GsaI CCCAGC 1 cut(s) 1893
GsuI CTGGAG 5 cut(s) 591, 2550, 3009, 3646, 4368
HaeII RGCGCY 1 cut(s) 211
HapII CCGG 3 cut(s) 1751, 2772, 3592
HgaI GACGC 2 cut(s) 27, 34
HhaI GCGC 3 cut(s) 177, 210, 3473
Hin1I GRCGYC 1 cut(s) 38
Hin6I GCGC 3 cut(s) 175, 208, 3471
HinP1I GCGC 3 cut(s) 175, 208, 3471
HincII GTYRAC 6 cut(s) 1026, 1955, 2686, 2799, 2869, 3546
HindII GTYRAC 6 cut(s) 1026, 1955, 2686, 2799, 2869, 3546
HindIII AAGCTT 4 cut(s) 2452, 2842, 3140, 3248
HpaI GTTAAC 1 cut(s) 2799
HpaII CCGG 3 cut(s) 1751, 2772, 3592
Hpy99I CGWCG 4 cut(s) 28, 70, 143, 4374
HpyCH4III ACNGT 9 cut(s) 443, 889, 1037, 1478, 2312, 2816, 2866, 3183, 3490
HpyCH4IV ACGT 6 cut(s) 258, 1531, 1621, 1691, 3882, 4369
HpySE526I ACGT 6 cut(s) 258, 1531, 1621, 1691, 3882, 4369
Hsp92I GRCGYC 1 cut(s) 38
HspAI GCGC 3 cut(s) 175, 208, 3471
Kpn2I TCCGGA 1 cut(s) 1750
Ksp22I TGATCA 3 cut(s) 1177, 3223, 3451
KspAI GTTAAC 1 cut(s) 2799
LmnI GCTCC 8 cut(s) 105, 601, 1052, 1060, 3091, 3594, 3617, 3908
MabI ACCWGGT 2 cut(s) 78, 4140
MaeII ACGT 6 cut(s) 258, 1531, 1621, 1691, 3882, 4369
MfeI CAATTG 1 cut(s) 1665
MflI RGATCY 2 cut(s) 3929, 4460
MhlI GDGCHC 5 cut(s) 244, 1057, 2068, 2745, 3574
MlsI TGGCCA 4 cut(s) 2106, 2318, 2734, 2923
MluNI TGGCCA 4 cut(s) 2106, 2318, 2734, 2923
MlyI GAGTC 5 cut(s) 176, 2758, 3362, 3615, 4440
MmeI TCCRAC 5 cut(s) 816, 1084, 2604, 3356, 3495
Mox20I TGGCCA 4 cut(s) 2106, 2318, 2734, 2923
Mph1103I ATGCAT 3 cut(s) 1354, 2305, 3310
MroI TCCGGA 1 cut(s) 1750
MroXI GAANNNNTTC 1 cut(s) 2945
MscI TGGCCA 4 cut(s) 2106, 2318, 2734, 2923
MslI CAYNNNNRTG 2 cut(s) 621, 2389
Msp20I TGGCCA 4 cut(s) 2106, 2318, 2734, 2923
MspA1I CMGCKG 5 cut(s) 1661, 1927, 2173, 2423, 3764
MspI CCGG 3 cut(s) 1751, 2772, 3592
MspR9I CCNGG 8 cut(s) 80, 1323, 1718, 3593, 3664, 4142, 4302, 4329
MunI CAATTG 1 cut(s) 1665
Mva1269I GAATGC 3 cut(s) 1113, 3339, 4123
MvaI CCWGG 7 cut(s) 80, 1323, 1718, 3664, 4142, 4302, 4329
MvnI CGCG 2 cut(s) 177, 4575
NciI CCSGG 1 cut(s) 3593
NdeI CATATG 4 cut(s) 532, 825, 1633, 3639
NmeAIII GCCGAG 1 cut(s) 2764
NmuCI GTSAC 4 cut(s) 437, 469, 1697, 2425
NsiI ATGCAT 3 cut(s) 1354, 2305, 3310
NspI RCATGY 4 cut(s) 1315, 1453, 2623, 3624
NspV TTCGAA 1 cut(s) 726
PaeI GCATGC 2 cut(s) 1315, 1453
PaeR7I CTCGAG 1 cut(s) 3603
PagI TCATGA 2 cut(s) 991, 2875
PaqCI CACCTGC 1 cut(s) 3414
PceI AGGCCT 2 cut(s) 2780, 3382
PciI ACATGT 1 cut(s) 2619
PcsI WCGNNNNNNNCGW 1 cut(s) 1618
PctI GAATGC 3 cut(s) 1113, 3339, 4123
PdmI GAANNNNTTC 1 cut(s) 2945
PflMI CCANNNNNTGG 4 cut(s) 2406, 3084, 3371, 3849
PfoI TCCNGGA 1 cut(s) 1716
Ple19I CGATCG 1 cut(s) 4396
PleI GAGTC 5 cut(s) 176, 2757, 3362, 3614, 4439
PpsI GAGTC 5 cut(s) 176, 2757, 3362, 3614, 4439
Ppu21I YACGTR 2 cut(s) 1532, 3883
PscI ACATGT 1 cut(s) 2619
Psp124BI GAGCTC 1 cut(s) 1057
Psp6I CCWGG 7 cut(s) 78, 1321, 1716, 3662, 4140, 4300, 4327
PspFI CCCAGC 1 cut(s) 1889
PspGI CCWGG 7 cut(s) 78, 1321, 1716, 3662, 4140, 4300, 4327
PspXI VCTCGAGB 1 cut(s) 3603
PstI CTGCAG 2 cut(s) 1962, 4093
PstNI CAGNNNCTG 4 cut(s) 2288, 3032, 3371, 4147
PsuI RGATCY 2 cut(s) 3929, 4460
PvuI CGATCG 1 cut(s) 4396
PvuII CAGCTG 3 cut(s) 1661, 2173, 2423
RsaI GTAC 6 cut(s) 47, 1595, 1896, 2648, 3198, 3794
RsaNI GTAC 6 cut(s) 46, 1594, 1895, 2647, 3197, 3793
RseI CAYNNNNRTG 2 cut(s) 621, 2389
SacI GAGCTC 1 cut(s) 1057
ScaI AGTACT 1 cut(s) 47
SchI GAGTC 5 cut(s) 176, 2758, 3362, 3615, 4440
ScrFI CCNGG 8 cut(s) 80, 1323, 1718, 3593, 3664, 4142, 4302, 4329
SduI GDGCHC 5 cut(s) 244, 1057, 2068, 2745, 3574
SexAI ACCWGGT 2 cut(s) 78, 4140
SfcI CTRYAG 9 cut(s) 678, 872, 1958, 2004, 2463, 3187, 4011, 4089, 4261
Sfr274I CTCGAG 1 cut(s) 3603
SfuI TTCGAA 1 cut(s) 726
SinI GGWCC 3 cut(s) 82, 151, 2036
SlaI CTCGAG 1 cut(s) 3603
SmiMI CAYNNNNRTG 2 cut(s) 621, 2389
SmlI CTYRAG 4 cut(s) 707, 1879, 2572, 3603
SmoI CTYRAG 4 cut(s) 707, 1879, 2572, 3603
SnaBI TACGTA 1 cut(s) 1532
SphI GCATGC 2 cut(s) 1315, 1453
SseBI AGGCCT 2 cut(s) 2780, 3382
SstI GAGCTC 1 cut(s) 1057
StuI AGGCCT 2 cut(s) 2780, 3382
StyD4I CCNGG 8 cut(s) 78, 1321, 1716, 3591, 3662, 4140, 4300, 4327
StyI CCWWGG 8 cut(s) 1251, 1683, 3083, 3297, 4193, 4200, 4527, 4590
TaaI ACNGT 9 cut(s) 443, 889, 1037, 1478, 2312, 2816, 2866, 3183, 3490
TaiI ACGT 6 cut(s) 261, 1534, 1624, 1694, 3885, 4372
TaqII GACCGA 3 cut(s) 354, 3408, 3525
TatI WGTACW 4 cut(s) 45, 1593, 3196, 3792
TauI GCSGC 4 cut(s) 111, 180, 1927, 4544
TseFI GTSAC 4 cut(s) 437, 469, 1697, 2425
Tsp45I GTSAC 4 cut(s) 437, 469, 1697, 2425
TspGWI ACGGA 6 cut(s) 185, 227, 903, 1059, 4258, 4298
Van91I CCANNNNNTGG 4 cut(s) 2406, 3084, 3371, 3849
VneI GTGCAC 1 cut(s) 3570
VpaK11BI GGWCC 3 cut(s) 82, 151, 2036
XagI CCTNNNNNAGG 2 cut(s) 89, 158
XapI RAATTY 4 cut(s) 1405, 1743, 3352, 3754
XbaI TCTAGA 3 cut(s) 2359, 2596, 3556
XceI RCATGY 4 cut(s) 1315, 1453, 2623, 3624
XcmI CCANNNNNNNNNTGG 2 cut(s) 3085, 3372
XhoI CTCGAG 1 cut(s) 3603
XmiI GTMKAC 1 cut(s) 881
XmnI GAANNNNTTC 1 cut(s) 2945
ZrmI AGTACT 1 cut(s) 47
Zsp2I ATGCAT 3 cut(s) 1354, 2305, 3310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.