pycom07g04450

Phosphoinositide 3-kinase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001323
Physical Location & Seq
Forward (+)
3843457 .. 3850789
7333 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g04450.1

Sequence Viewer

Length: 4614 bp
ATGGGGAACAAGATCGCTCGAACGACGCAAGTGTCGGCGTCGGAGTATTACCTCCACGACCTGCCGTCGTCCTACAACGTGGTCTTGAAGGAGGTTCTAGGGCGGAGCCGCTTCTTCAAGTCGATTGAGTGCAAGCACGACGAGGGTTTGGTCCTCGTCAAGGTCTATTTCAAGCGCGGCGACTCCATTGATCTCCGCGAATACGAGCGCCGTCTTTGCCACATCAAGGAAACCTTCCGTGCTCTCGATCACCCTCACGTTTGGCCCTTCCAGTTTTGGCAAGAGACAGATAAAGCAGCTTATCTCGTGAGGCAATTCTTCTTCAACAATCTGCATGACCGATTAAGCACACGCCCTTTTCTCAGTCTAATCGAGAAGAAATGGTTGGCTTTTCAGTTACTTCTTGCTCTGAAGCAGTGCCATGACAAAGGAATATGTCACGGTGATATTAAGTGCGAGAATGTATTGGTGACTTCTTGGAATTGGCTTTACCTTGCTGACTTCGCATCTTTCAAACCCACCTACATACCATATGACGATCCTTCTGATTTCTCTTTTTTCTTTGACACTGGAGGAAGAAGGCTTTGTTATCTTGCTCCTGAGAGATTTTATGAGCATGGAGGTGAGATGCAGGTTGCACAAGATGCACCATTAAGACCATCTATGGACATATTTGCTGTAGGGTGTGTAATTGCTGAGCTTTTCCTTGAGGGCCAGCCACTATTCGAACTCTCTCAACTTCTTGCTTATCGTAGAGGGCAGTACGATCCTAGCCAACTTCTTGAAAAGATACCGGATTATGGGATTCGCAAGATGATACTTCATATGATTCAGTTGGAACCAGAGTTGCGACTTTCTGCTGATAGCTACCTGCAGGAGTATACAACTGTTGTGTTTCCAAGTTACTTCTCTCCGTTTCTGCATAATTTTCATTGTTTCTGGAATCCCCTTCATTCAGATATGAGGGTTGCACTTTGCCAGAGTGTTTTTCATGAGATACTTAAACAAATGATGAGCAGTAGGTCAACTGAGGACACTGGTGGAAACGGACTGGGAACTCCTTCAAGTGCCAATGCACTTAGTGGTAGAACTTCTCAGGAGGCGGTTGGAATGCAGAACAAAAGTTTGACAAAGGGCTCGTTTCCAAAGAGAGAAGAGATGGGTAAAGGCTTAAAATGTGATCAATTAGAACTTCTTGGTGATATCAATACTCTTCTTCGGGATGTTAAACAAAGTAATAATTACTCTGGTACCAAGCCAATGCTGAATGATATTCCTGATTCTGCGTTTTCCCCGAATCTTGGAAACTATGGCATGCAATCTCCTGGTGATTTACTTCAATCCATCTCCAATGCATTTAGGAGAAATGATCATCCTTTTCTGAAAAAGATTACATTGAATGATTTTAATTCATTGATGTCCAAGTATGACAGCCAGTCAGATACCTTTGGCATGCCGTTTCTGCCATTACCTGAAGACAGTATCAGATGTGAGGGCATGGTTCTGATTACCTCGCTGCTCTGTTCCTGCATACGTAATGTAAAATTGCCTCATTTAAGAAGGAGGGCCATACTCTTGCTGAAATCGTCTGCCTTGTACATTGATGATGAAGATCGTTTGCAACGTGTAATTCCATATGTTGTTGCAATGCTTTCAGATTCAGCCGCAATTGTGCGTTGTGCTGCCTTGGAGACGTTGTGTGACATTCTCCCTCTAGTCCGGGATTTTCCACCCAGTGATGCAAAAATTTTTCCGGAGTATATATTGCCAATGCTTTCCACGCTTCCCGATGACCCAGAGGAAAGCGTGAGAATATGTTATGCCAGTAATATAGCTAAGCTGGCATTAACTGCTTATGGTTTCTTGGTTCACTCAATACGCTTGAGTGAGGCTGGGGTACTTGATGAATTAACTTCTGCAAAAAAGCCGCTGCTTGCAGTGTTGAGGAAATCTATAGCTGAGGTCATTCAAGAACTTGTTATGGGTCCAAAGAAAACTCCAAATATCAGGAGAGCACTCCTTCAGGACATTAGCAACCTATGCTGCTTCTTTGGCCAGAGACAGAGTAATGATTTTTTGTTACCCATTCTCCCTGCTTTTCTGAATGATCGAGATGAGCAGCTGAGAGCAGTATTCTATGGGCAAATTGTGTATGTGTGTTTCTTTGTGGGTCAAAGAAGTGTGGAAGAATATCTTTTACCTTATATTGAGCAGGCTGTAAGTGATGTTACAGAGGCTGTCATTGTCAATGCATTGGACTGTCTGGCCATTTTGTGCAAAAGTGGTTTCTTGAGGAAGAGGATACTTCTAGAAATGATTGAGCGTGCATTTCCATTGTTATGCTATCCCAGTCAGTGGGTAAGAAGGTCAGCTGTCACTTTTATTGCAGCCAGTAGTGAAAGCTTAGGTGCTGTAGATTCCTATGTCTTTCTAGCCCCTGTTATTCGGCCCTTACTCCGCAGACAGCCAGCATCTCTTGCTTCTGAAAAGGCTCTCCTTTCGTCTTTAAAACCTCCAGTCTCAAGACAGGTTTTTTACCAAGTTCTAGAAAATGCAAGGAGTTCAGACATGTTGGAAAGACAGAGAAAGATATGGTACAACTCTTGGCCTCAGTCTAAACAATGGGAAAGTGTTGATTTACTTCAGCAAGGGGTTGAGGAACTGAGTTCAACAAGGAGCTGGCCAGACAAGCACCAGAGTCCTGAAAATCATAAACTTACTGGAAAGGCCTTGCAACAAGGAGAGTTAACTGAATGTGATGATGGTGAGGATAAGTTGAGAAGTATGGGAAGCTTTACACGCGCTTCTAGTACAGTTGACATTCACGATCCTTTGTCCTCTGAAAAAATGCAATATTCAGGTTTTATGTGGCCACAGGGAAGTTATGTGAATAGCTTCATGTGCGATAAATCATCAGTTGGCATACCTTTATACTCCTTTAGCATGGACAAGCGAGCAGCGGGAGTTCCTCCTCCAGCATCTGATTCTCCTTCACAAGTGAATTCAGTAGGAATTGGTACATCATCCATGCCTTGGATAGAGCCAGTAAATAAATCATTTAGTTTGGCTAGTTCTGTTCCAGCACCTAAGCTTGTGTCAGGATCATTCAATTTTGGCAATGGCTCCAAACAGTTCTATAGAGTTGTACATGAGCCTGATGGTAGGGATAATGATCAAACCGCCTTTGCTAATAGCAAGCTTCAAAATATGGGATTATCTGGCACTACAAAAGGAAGTGCCATACCCTTGGAAGATGCATCCACTTCAAGTGATCCAACAGGAATGCCGTCTTCTGCACGAAATTCATCAATTCCAGACTCTGGTTGGAGGCCTCGGGGGGTGTTGGTTGCTCACCTGCAAGAACATCGGTCTGCTGTCAATGACATAGCCATTTCAACCGATCATAGCTTTTTTGTGAGCGCATCTGATGATTCCACTGTTAAGGTGTGGGATTCAAGAAAGTTGGAAAAGGACATATCATTTCGGTCAAGGTTAACTTATCATCTAGAGGGAAGCCGTGCACTGTGCAGTGCAATGCTCCGGGGGTCTGCTCGAGTCGTTGTAGGAGCATGTGATGGCATGATACATATGTTTTCTGTTGATTACATCTCCAGGGGACTTGGTAATGTGGTTGAAAAGTATTCAGGTGTTGCTGATATCAAGAAGAAGGATATAAAGGAGGGTGCCATATTGAGCTTGTTAAATTTCTCAGCGGATAACTGCACAAATCAGATGGTTATGTACAGCACCCAAAATTGTGGTATCCATCTTTGGGATACAAGGTTGAACACCAATTCTTGGACATTGAGAGCAACTCCTGAGGAGGGCTATGTGTCTTCGCTGGTAACAGGCTCCTGTGAAAATTGGTTTGTGTCAGGATCTTCGAGGGGTGTGCTTACTCTTTGGGATATGAGGTTCCTTATTCCTGTAAACTCATGGCAGTATTCTTCTGTTTGCCCAATAGAGAAAATGTGCCTTTTTCTTCCTCCACCTAATGCCTCCGTGTCTGCTGCTGCAAGGCCCCTTATTTATGTTGCTGCAGGTTGCAACGAAGTTTCTCTTTGGAATGCTGAAAATGGAAGCTGCCACCAGGTGCTGAGAGTGGCAAGTTACGAAAGTGATGCTGAAATGTCTGAAGTGCCTTGGGCCTTGGCTAAGTCATCAAGTAAAAGTTCCAAACCAGATCTGAGACGGAATGGCAATTCCCACTACAGAGTTGATGAACTAAATGAACCCACTCCTCGTCTTCCTGGAATCCGTTCATTGCTGCCCTTACCTGGGGGTGATTTGATAACTGGAGGAACCGATTTGAAGATACGTCGATGGGATCATTACAGCCCCGGTCGAAGTTACTCAATTTGTGGGCCAAACTTGAAGGGAGTTGGTAATGATGATTTCTATGCAACAAGATCTAGCTTTGGTGTGGAAGTTGTGCAGGAGACAAAAAGACGACCTCTAACATCAAAGCTGACAGCCAAGGCAGTTCTTGCGGCTGCTGCCACTGATTCTGCTGGCTGCCACCGCGATTCAATCCTATCATTGGCCTCGGTTAAGTTAAATCAGAGACACTTAATATCAAGCGGTAGAGATGGGGCAATTAAGGTTTGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000011 GO:0000151 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005737 GO:0005768 GO:0005770 GO:0005942 GO:0006355 GO:0006357 GO:0006464 GO:0006468 GO:0006605 GO:0006623 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006886 GO:0006914 GO:0006996 GO:0007033 GO:0007034 GO:0007154 GO:0007165 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009555 GO:0009846 GO:0009856 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010008 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0016236 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019219 GO:0019222 GO:0019538 GO:0019898 GO:0022414 GO:0023052 GO:0030242 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031410 GO:0031461 GO:0031967 GO:0031975 GO:0031982 GO:0032182 GO:0032501 GO:0032502 GO:0032507 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0033036 GO:0033365 GO:0034067 GO:0034243 GO:0034271 GO:0034272 GO:0034613 GO:0035032 GO:0035556 GO:0036211 GO:0042886 GO:0043130 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0044706 GO:0045053 GO:0045184 GO:0045185 GO:0045324 GO:0045893 GO:0045935 GO:0045944 GO:0046907 GO:0048015 GO:0048017 GO:0048229 GO:0048308 GO:0048518 GO:0048522 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051235 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051651 GO:0051704 GO:0051716 GO:0060255 GO:0061695 GO:0061919 GO:0065007 GO:0065008 GO:0070727 GO:0071561 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0080008 GO:0080090 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0120095 GO:0140096 GO:1901564 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04136 ko04138 ko04140 ko04371 map04136 map04138 map04140 map04371
Pfam Domains
Protein Families
HEAT Pkinase WD40

Protein Analysis

1538

Amino Acids

171.87

Weight (kDa)

7.3

Isoelectric Point (pI)

52.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 30 - 292 2.3e-19 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 30 - 239 3.7e-06 Protein tyrosine and serine/threonine kinase
VPS15-like_hel PF22956 503 - 848 1.2e-120 VPS15-like, helical domain
Beta-prop_WDR5 PF25175 1113 - 1200 9e-09 WDR5 beta-propeller domain
WD40_Prp19 PF24814 1113 - 1318 1.9e-08 Prp19 WD40 domain
WD40 PF00400 1114 - 1151 9.9e-09 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 1116 - 1199 9.6e-06 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 1117 - 1312 3.6e-08 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 1124 - 1325 2.5e-06 WDHD1 first WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3363
AasI GACNNNNNNGTC 1 cut(s) 31
Acc36I ACCTGC 5 cut(s) 69, 622, 879, 3363, 4031
Acc65I GGTACC 1 cut(s) 1250
AccB1I GGYRCC 2 cut(s) 1250, 3683
AccB7I CCANNNNNTGG 4 cut(s) 2355, 3033, 3320, 3798
AccI GTMKAC 1 cut(s) 881
AccII CGCG 4 cut(s) 177, 198, 2802, 4524
AccIII TCCGGA 1 cut(s) 1753
AclWI GGATC 7 cut(s) 533, 761, 2822, 3109, 3266, 3886, 4335
AcoI YGGCCR 4 cut(s) 2053, 2265, 2681, 2870
AcsI RAATTY 4 cut(s) 1746, 3001, 3301, 3703
AcuI CTGAAG 5 cut(s) 431, 1494, 2006, 2627, 4155
AcyI GRCGYC 1 cut(s) 38
AdeI CACNNNGTG 3 cut(s) 1082, 1736, 4093
AfaI GTAC 9 cut(s) 764, 1252, 1598, 1899, 2597, 2812, 3019, 3147, 3743
AflIII ACRYGT 2 cut(s) 1624, 2568
AhdI GACNNNNNGTC 2 cut(s) 64, 1435
AjnI CCWGG 5 cut(s) 1324, 3611, 4089, 4249, 4276
AjuI GAANNNNNNNTTGG 2 cut(s) 368, 400
AloI GAACNNNNNNTCC 2 cut(s) 3899, 3931
Alw21I GWGCWC 3 cut(s) 244, 2017, 3523
Alw26I GTCTC 7 cut(s) 278, 1685, 2053, 2524, 4183, 4433, 4558
Alw44I GTGCAC 1 cut(s) 3519
AlwI GGATC 7 cut(s) 533, 761, 2822, 3109, 3266, 3886, 4335
AlwNI CAGNNNCTG 4 cut(s) 2237, 2981, 3320, 4096
Ama87I CYCGRG 2 cut(s) 3333, 3552
Aor13HI TCCGGA 1 cut(s) 1753
ApaLI GTGCAC 1 cut(s) 3519
ApoI RAATTY 4 cut(s) 1746, 3001, 3301, 3703
Asp700I GAANNNNTTC 3 cut(s) 1060, 2894, 4258
Asp718I GGTACC 1 cut(s) 1250
AspLEI GCGC 4 cut(s) 177, 210, 2804, 3422
AspS9I GGNCC 9 cut(s) 151, 264, 712, 1566, 1985, 2448, 4020, 4146, 4364
AsuC2I CCSGG 3 cut(s) 1721, 3542, 4341
AsuHPI GGTGA 9 cut(s) 242, 455, 481, 635, 1211, 1340, 2777, 3344, 4295
AsuII TTCGAA 1 cut(s) 726
AvaI CYCGRG 2 cut(s) 3333, 3552
AvaII GGWCC 2 cut(s) 151, 1985
AxyI CCTNAGG 1 cut(s) 3819
BaeGI GKGCMC 1 cut(s) 3523
BaeI ACNNNNGTAYC 4 cut(s) 1234, 1267, 3575, 3608
BalI TGGCCA 4 cut(s) 2055, 2267, 2683, 2872
BanI GGYRCC 2 cut(s) 1250, 3683
BanII GRGCYC 1 cut(s) 1139
BauI CACGAG 1 cut(s) 305
BbsI GAAGAC 4 cut(s) 1482, 3282, 3828, 4238
Bbv12I GWGCWC 3 cut(s) 244, 2017, 3523
BbvCI CCTCAGC 1 cut(s) 1959
BceAI ACGGC 5 cut(s) 49, 195, 1441, 3271, 3501
BcgI CGANNNNNNTGC 6 cut(s) 3347, 3381, 3874, 3908, 4103, 4137
BciT130I CCWGG 5 cut(s) 1326, 3613, 4091, 4251, 4278
BciVI GTATCC 3 cut(s) 2295, 3769, 3773
BclI TGATCA 3 cut(s) 1180, 1369, 3172
BcnI CCSGG 3 cut(s) 1721, 3542, 4341
BcoDI GTCTC 7 cut(s) 278, 1685, 2053, 2524, 4183, 4433, 4558
BfmI CTRYAG 7 cut(s) 678, 872, 1953, 2412, 3136, 4038, 4210
BfoI RGCGCY 1 cut(s) 211
BfuAI ACCTGC 5 cut(s) 69, 622, 879, 3363, 4031
BfuI GTATCC 3 cut(s) 2295, 3769, 3773
BglII AGATCT 2 cut(s) 4183, 4409
BlpI GCTNAGC 2 cut(s) 696, 1836
Bme1390I CCNGG 8 cut(s) 1326, 1721, 3542, 3613, 4091, 4251, 4278, 4341
Bme18I GGWCC 2 cut(s) 151, 1985
BmeRI GACNNNNNGTC 2 cut(s) 64, 1435
BmeT110I CYCGRG 2 cut(s) 3333, 3552
BmgT120I GGNCC 9 cut(s) 151, 264, 712, 1566, 1985, 2448, 4020, 4146, 4364
BmrFI CCNGG 8 cut(s) 1326, 1721, 3542, 3613, 4091, 4251, 4278, 4341
BmrI ACTGGG 3 cut(s) 1061, 1728, 2343
BmuI ACTGGG 3 cut(s) 1061, 1728, 2343
BpiI GAAGAC 4 cut(s) 1482, 3282, 3828, 4238
BplI GAGNNNNNCTC 2 cut(s) 3799, 3831
BpmI CTGGAG 5 cut(s) 591, 2499, 2958, 3595, 4317
Bpu10I CCTNAGC 3 cut(s) 1959, 2404, 3087
Bpu1102I GCTNAGC 2 cut(s) 696, 1836
Bpu14I TTCGAA 1 cut(s) 726
BpuEI CTTGAG 4 cut(s) 728, 1903, 2311, 2506
BpuMI CCSGG 3 cut(s) 1721, 3542, 4341
BsaAI YACGTR 1 cut(s) 1535
BsaBI GATNNNNATC 2 cut(s) 1611, 3171
BsaHI GRCGYC 1 cut(s) 38
BsaWI WCCGGW 2 cut(s) 793, 1753
BsaXI ACNNNNNCTCC 4 cut(s) 1682, 1712, 2073, 2103
Bse21I CCTNAGG 1 cut(s) 3819
Bse3DI GCAATG 4 cut(s) 1653, 3124, 3540, 4262
Bse8I GATNNNNATC 2 cut(s) 1611, 3171
BseAI TCCGGA 1 cut(s) 1753
BseBI CCWGG 5 cut(s) 1326, 3613, 4091, 4251, 4278
BseGI GGATG 4 cut(s) 1228, 1372, 3023, 3257
BseJI GATNNNNATC 2 cut(s) 1611, 3171
BseMI GCAATG 4 cut(s) 1653, 3124, 3540, 4262
BseRI GAGGAG 3 cut(s) 2961, 3836, 4230
BseSI GKGCMC 1 cut(s) 3523
BseYI CCCAGC 1 cut(s) 1892
BsgI GTGCAG 4 cut(s) 3279, 3547, 3706, 4454
Bsh1236I CGCG 4 cut(s) 177, 198, 2802, 4524
Bsh1285I CGRYCG 1 cut(s) 4345
BshNI GGYRCC 2 cut(s) 1250, 3683
BsiEI CGRYCG 1 cut(s) 4345
BsiHKAI GWGCWC 3 cut(s) 244, 2017, 3523
BsiHKCI CYCGRG 2 cut(s) 3333, 3552
BsiSI CCGG 5 cut(s) 794, 1720, 1754, 3541, 4341
BslFI GGGAC 1 cut(s) 3630
BsmAI GTCTC 7 cut(s) 278, 1685, 2053, 2524, 4183, 4433, 4558
BsmBI CGTCTC 2 cut(s) 1685, 4183
BsmFI GGGAC 1 cut(s) 3630
BsmI GAATGC 3 cut(s) 1116, 3288, 4072
BsoBI CYCGRG 2 cut(s) 3333, 3552
Bsp119I TTCGAA 1 cut(s) 726
Bsp1286I GDGCHC 4 cut(s) 244, 1139, 2017, 3523
Bsp13I TCCGGA 1 cut(s) 1753
Bsp1407I TGTACA 3 cut(s) 1596, 3145, 3741
Bsp1720I GCTNAGC 2 cut(s) 696, 1836
BspEI TCCGGA 1 cut(s) 1753
BspFNI CGCG 4 cut(s) 177, 198, 2802, 4524
BspHI TCATGA 1 cut(s) 991
BspMAI CTGCAG 2 cut(s) 876, 4042
BspMI ACCTGC 5 cut(s) 69, 622, 879, 3363, 4031
BspPI GGATC 7 cut(s) 533, 761, 2822, 3109, 3266, 3886, 4335
BspT104I TTCGAA 1 cut(s) 726
BspT107I GGYRCC 2 cut(s) 1250, 3683
BsrDI GCAATG 4 cut(s) 1653, 3124, 3540, 4262
BsrGI TGTACA 3 cut(s) 1596, 3145, 3741
BssNAI GTATAC 1 cut(s) 882
BssNI GRCGYC 1 cut(s) 38
BssSI CACGAG 1 cut(s) 305
BssT1I CCWWGG 6 cut(s) 1686, 3032, 3246, 4142, 4149, 4476
Bst1107I GTATAC 1 cut(s) 882
Bst2BI CACGAG 1 cut(s) 305
Bst2UI CCWGG 5 cut(s) 1326, 3613, 4091, 4251, 4278
Bst4CI ACNGT 8 cut(s) 443, 889, 1481, 2261, 2815, 3132, 3439, 3525
Bst6I CTCTTC 3 cut(s) 1149, 1218, 2291
BstACI GRCGYC 1 cut(s) 38
BstAPI GCANNNNNTGC 5 cut(s) 644, 1850, 2040, 2477, 4487
BstAUI TGTACA 3 cut(s) 1596, 3145, 3741
BstBAI YACGTR 1 cut(s) 1535
BstBI TTCGAA 1 cut(s) 726
BstENI CCTNNNNNAGG 1 cut(s) 158
BstF5I GGATG 4 cut(s) 1228, 1372, 3023, 3257
BstFNI CGCG 4 cut(s) 177, 198, 2802, 4524
BstH2I RGCGCY 1 cut(s) 211
BstHHI GCGC 4 cut(s) 177, 210, 2804, 3422
BstMAI GTCTC 7 cut(s) 278, 1685, 2053, 2524, 4183, 4433, 4558
BstMCI CGRYCG 1 cut(s) 4345
BstNI CCWGG 5 cut(s) 1326, 3613, 4091, 4251, 4278
BstNSI RCATGY 4 cut(s) 1318, 1456, 2572, 3573
BstSCI CCNGG 8 cut(s) 1324, 1719, 3540, 3611, 4089, 4249, 4276, 4339
BstSFI CTRYAG 7 cut(s) 678, 872, 1953, 2412, 3136, 4038, 4210
BstSLI GKGCMC 1 cut(s) 3523
BstSNI TACGTA 1 cut(s) 1535
BstUI CGCG 4 cut(s) 177, 198, 2802, 4524
BstV2I GAAGAC 4 cut(s) 1482, 3282, 3828, 4238
BstX2I RGATCY 3 cut(s) 3878, 4183, 4409
BstXI CCANNNNNNTGG 2 cut(s) 3247, 3758
BstYI RGATCY 3 cut(s) 3878, 4183, 4409
BstZ17I GTATAC 1 cut(s) 882
Bsu36I CCTNAGG 1 cut(s) 3819
BsuI GTATCC 3 cut(s) 2295, 3769, 3773
BtsCI GGATG 4 cut(s) 1228, 1372, 3023, 3257
BtsI GCAGTG 3 cut(s) 422, 1944, 3535
BveI ACCTGC 5 cut(s) 69, 622, 879, 3363, 4031
CaiI CAGNNNCTG 4 cut(s) 2237, 2981, 3320, 4096
CciI TCATGA 1 cut(s) 991
CfoI GCGC 4 cut(s) 177, 210, 2804, 3422
Cfr13I GGNCC 9 cut(s) 151, 264, 712, 1566, 1985, 2448, 4020, 4146, 4364
CseI GACGC 2 cut(s) 27, 34
CsiI ACCWGGT 1 cut(s) 4089
Csp6I GTAC 9 cut(s) 763, 1251, 1597, 1898, 2596, 2811, 3018, 3146, 3742
CviQI GTAC 9 cut(s) 763, 1251, 1597, 1898, 2596, 2811, 3018, 3146, 3742
DraI TTTAAA 1 cut(s) 2508
DraIII CACNNNGTG 3 cut(s) 1082, 1736, 4093
DrdI GACNNNNNNGTC 1 cut(s) 31
DriI GACNNNNNGTC 2 cut(s) 64, 1435
DseDI GACNNNNNNGTC 1 cut(s) 31
EaeI YGGCCR 4 cut(s) 2053, 2265, 2681, 2870
Eam1104I CTCTTC 3 cut(s) 1149, 1218, 2291
Eam1105I GACNNNNNGTC 2 cut(s) 64, 1435
EarI CTCTTC 3 cut(s) 1149, 1218, 2291
EciI GGCGGA 1 cut(s) 118
Eco105I TACGTA 1 cut(s) 1535
Eco130I CCWWGG 6 cut(s) 1686, 3032, 3246, 4142, 4149, 4476
Eco147I AGGCCT 2 cut(s) 2729, 3331
Eco24I GRGCYC 1 cut(s) 1139
Eco32I GATATC 2 cut(s) 1204, 3658
Eco47I GGWCC 2 cut(s) 151, 1985
Eco57I CTGAAG 5 cut(s) 431, 1494, 2006, 2627, 4155
Eco81I CCTNAGG 1 cut(s) 3819
Eco88I CYCGRG 2 cut(s) 3333, 3552
EcoNI CCTNNNNNAGG 1 cut(s) 158
EcoO109I RGGNCCY 1 cut(s) 4020
EcoRI GAATTC 1 cut(s) 3001
EcoRII CCWGG 5 cut(s) 1324, 3611, 4089, 4249, 4276
EcoRV GATATC 2 cut(s) 1204, 3658
EcoT14I CCWWGG 6 cut(s) 1686, 3032, 3246, 4142, 4149, 4476
EcoT22I ATGCAT 3 cut(s) 1357, 2254, 3259
EcoT38I GRGCYC 1 cut(s) 1139
ErhI CCWWGG 6 cut(s) 1686, 3032, 3246, 4142, 4149, 4476
Esp3I CGTCTC 2 cut(s) 1685, 4183
FalI AAGNNNNNCTT 8 cut(s) 218, 250, 2178, 2210, 3481, 3513, 4044, 4076
FaqI GGGAC 1 cut(s) 3630
FauI CCCGC 1 cut(s) 2953
FauNDI CATATG 4 cut(s) 532, 825, 1636, 3588
FbaI TGATCA 3 cut(s) 1180, 1369, 3172
FblI GTMKAC 1 cut(s) 881
FokI GGATG 4 cut(s) 1235, 1359, 3010, 3244
FriOI GRGCYC 1 cut(s) 1139
GlaI GCGC 4 cut(s) 176, 209, 2803, 3421
GsaI CCCAGC 1 cut(s) 1896
GsuI CTGGAG 5 cut(s) 591, 2499, 2958, 3595, 4317
HaeII RGCGCY 1 cut(s) 211
HapII CCGG 5 cut(s) 794, 1720, 1754, 3541, 4341
HgaI GACGC 2 cut(s) 27, 34
HhaI GCGC 4 cut(s) 177, 210, 2804, 3422
Hin1I GRCGYC 1 cut(s) 38
Hin6I GCGC 4 cut(s) 175, 208, 2802, 3420
HinP1I GCGC 4 cut(s) 175, 208, 2802, 3420
HincII GTYRAC 4 cut(s) 1026, 2748, 2818, 3495
HindII GTYRAC 4 cut(s) 1026, 2748, 2818, 3495
HindIII AAGCTT 4 cut(s) 2401, 2791, 3089, 3197
HpaI GTTAAC 2 cut(s) 2748, 3495
HpaII CCGG 5 cut(s) 794, 1720, 1754, 3541, 4341
HphI GGTGA 9 cut(s) 242, 455, 481, 635, 1211, 1340, 2777, 3344, 4295
Hpy166II GTNNAC 8 cut(s) 882, 1026, 1870, 2748, 2818, 3495, 3521, 3931
Hpy8I GTNNAC 8 cut(s) 882, 1026, 1870, 2748, 2818, 3495, 3521, 3931
Hpy99I CGWCG 5 cut(s) 28, 43, 70, 143, 4323
HpyCH4III ACNGT 8 cut(s) 443, 889, 1481, 2261, 2815, 3132, 3439, 3525
HpyCH4IV ACGT 6 cut(s) 78, 258, 1534, 1624, 1694, 4318
HpySE526I ACGT 6 cut(s) 78, 258, 1534, 1624, 1694, 4318
Hsp92I GRCGYC 1 cut(s) 38
HspAI GCGC 4 cut(s) 175, 208, 2802, 3420
Kpn2I TCCGGA 1 cut(s) 1753
KpnI GGTACC 1 cut(s) 1254
Ksp22I TGATCA 3 cut(s) 1180, 1369, 3172
KspAI GTTAAC 2 cut(s) 2748, 3495
LmnI GCTCC 7 cut(s) 105, 601, 2676, 3128, 3543, 3566, 3857
MabI ACCWGGT 1 cut(s) 4089
MaeII ACGT 6 cut(s) 78, 258, 1534, 1624, 1694, 4318
MfeI CAATTG 1 cut(s) 1668
MflI RGATCY 3 cut(s) 3878, 4183, 4409
MhlI GDGCHC 4 cut(s) 244, 1139, 2017, 3523
MlsI TGGCCA 4 cut(s) 2055, 2267, 2683, 2872
MluNI TGGCCA 4 cut(s) 2055, 2267, 2683, 2872
MlyI GAGTC 4 cut(s) 176, 2707, 3311, 3564
MmeI TCCRAC 7 cut(s) 21, 816, 1087, 2553, 3299, 3305, 3444
Mox20I TGGCCA 4 cut(s) 2055, 2267, 2683, 2872
Mph1103I ATGCAT 3 cut(s) 1357, 2254, 3259
MroI TCCGGA 1 cut(s) 1753
MroXI GAANNNNTTC 3 cut(s) 1060, 2894, 4258
MscI TGGCCA 4 cut(s) 2055, 2267, 2683, 2872
MslI CAYNNNNRTG 2 cut(s) 621, 2338
Msp20I TGGCCA 4 cut(s) 2055, 2267, 2683, 2872
MspA1I CMGCKG 5 cut(s) 1930, 2122, 2372, 2960, 3713
MspI CCGG 5 cut(s) 794, 1720, 1754, 3541, 4341
MspR9I CCNGG 8 cut(s) 1326, 1721, 3542, 3613, 4091, 4251, 4278, 4341
MunI CAATTG 1 cut(s) 1668
Mva1269I GAATGC 3 cut(s) 1116, 3288, 4072
MvaI CCWGG 5 cut(s) 1326, 3613, 4091, 4251, 4278
MvnI CGCG 4 cut(s) 177, 198, 2802, 4524
NciI CCSGG 3 cut(s) 1721, 3542, 4341
NdeI CATATG 4 cut(s) 532, 825, 1636, 3588
NmuCI GTSAC 4 cut(s) 437, 469, 1700, 2374
NsiI ATGCAT 3 cut(s) 1357, 2254, 3259
NspI RCATGY 4 cut(s) 1318, 1456, 2572, 3573
NspV TTCGAA 1 cut(s) 726
PaeI GCATGC 2 cut(s) 1318, 1456
PaeR7I CTCGAG 1 cut(s) 3552
PagI TCATGA 1 cut(s) 991
PaqCI CACCTGC 1 cut(s) 3363
PceI AGGCCT 2 cut(s) 2729, 3331
PciI ACATGT 1 cut(s) 2568
PcsI WCGNNNNNNNCGW 1 cut(s) 1621
PctI GAATGC 3 cut(s) 1116, 3288, 4072
PdmI GAANNNNTTC 3 cut(s) 1060, 2894, 4258
PflMI CCANNNNNTGG 4 cut(s) 2355, 3033, 3320, 3798
PfoI TCCNGGA 2 cut(s) 1719, 4249
PleI GAGTC 4 cut(s) 176, 2706, 3311, 3563
PpsI GAGTC 4 cut(s) 176, 2706, 3311, 3563
Ppu21I YACGTR 1 cut(s) 1535
PscI ACATGT 1 cut(s) 2568
Psp6I CCWGG 5 cut(s) 1324, 3611, 4089, 4249, 4276
PspFI CCCAGC 1 cut(s) 1892
PspGI CCWGG 5 cut(s) 1324, 3611, 4089, 4249, 4276
PspPI GGNCC 9 cut(s) 151, 264, 712, 1566, 1985, 2448, 4020, 4146, 4364
PspXI VCTCGAGB 1 cut(s) 3552
PstI CTGCAG 2 cut(s) 876, 4042
PstNI CAGNNNCTG 4 cut(s) 2237, 2981, 3320, 4096
PsuI RGATCY 3 cut(s) 3878, 4183, 4409
PvuII CAGCTG 2 cut(s) 2122, 2372
RsaI GTAC 9 cut(s) 764, 1252, 1598, 1899, 2597, 2812, 3019, 3147, 3743
RsaNI GTAC 9 cut(s) 763, 1251, 1597, 1898, 2596, 2811, 3018, 3146, 3742
RseI CAYNNNNRTG 2 cut(s) 621, 2338
Sau96I GGNCC 9 cut(s) 151, 264, 712, 1566, 1985, 2448, 4020, 4146, 4364
SbfI CCTGCAGG 1 cut(s) 876
SchI GAGTC 4 cut(s) 176, 2707, 3311, 3564
ScrFI CCNGG 8 cut(s) 1326, 1721, 3542, 3613, 4091, 4251, 4278, 4341
SdaI CCTGCAGG 1 cut(s) 876
SduI GDGCHC 4 cut(s) 244, 1139, 2017, 3523
SexAI ACCWGGT 1 cut(s) 4089
SfcI CTRYAG 7 cut(s) 678, 872, 1953, 2412, 3136, 4038, 4210
Sfr274I CTCGAG 1 cut(s) 3552
SfuI TTCGAA 1 cut(s) 726
SinI GGWCC 2 cut(s) 151, 1985
SlaI CTCGAG 1 cut(s) 3552
SmiMI CAYNNNNRTG 2 cut(s) 621, 2338
SmlI CTYRAG 5 cut(s) 707, 1882, 2290, 2521, 3552
SmoI CTYRAG 5 cut(s) 707, 1882, 2290, 2521, 3552
SnaBI TACGTA 1 cut(s) 1535
SphI GCATGC 2 cut(s) 1318, 1456
Sse8387I CCTGCAGG 1 cut(s) 876
SseBI AGGCCT 2 cut(s) 2729, 3331
SspI AATATT 1 cut(s) 2855
StuI AGGCCT 2 cut(s) 2729, 3331
StyD4I CCNGG 8 cut(s) 1324, 1719, 3540, 3611, 4089, 4249, 4276, 4339
StyI CCWWGG 6 cut(s) 1686, 3032, 3246, 4142, 4149, 4476
TaaI ACNGT 8 cut(s) 443, 889, 1481, 2261, 2815, 3132, 3439, 3525
TaiI ACGT 6 cut(s) 81, 261, 1537, 1627, 1697, 4321
TaqII GACCGA 3 cut(s) 354, 3357, 3474
TatI WGTACW 4 cut(s) 1596, 2810, 3145, 3741
TauI GCSGC 5 cut(s) 111, 180, 1667, 1930, 4493
TseFI GTSAC 4 cut(s) 437, 469, 1700, 2374
Tsp45I GTSAC 4 cut(s) 437, 469, 1700, 2374
TspGWI ACGGA 6 cut(s) 227, 903, 1062, 3991, 4207, 4247
Van91I CCANNNNNTGG 4 cut(s) 2355, 3033, 3320, 3798
VneI GTGCAC 1 cut(s) 3519
VpaK11BI GGWCC 2 cut(s) 151, 1985
XagI CCTNNNNNAGG 1 cut(s) 158
XapI RAATTY 4 cut(s) 1746, 3001, 3301, 3703
XbaI TCTAGA 3 cut(s) 2308, 2545, 3505
XceI RCATGY 4 cut(s) 1318, 1456, 2572, 3573
XcmI CCANNNNNNNNNTGG 1 cut(s) 3321
XhoI CTCGAG 1 cut(s) 3552
XmiI GTMKAC 1 cut(s) 881
XmnI GAANNNNTTC 3 cut(s) 1060, 2894, 4258
Zsp2I ATGCAT 3 cut(s) 1357, 2254, 3259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.