Rh1BG090300

Phosphoinositide 3-kinase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0001323
Physical Location & Seq
Forward (+)
14297525 .. 14306167
8643 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG090300.1

Sequence Viewer

Length: 4038 bp
ATGCAAGTTGCACAAGATGCACCATTAAGACCATCTATGGATATCTTTGCTGTAGGGTGTGTGATTGCTGAGCTTTTTCTTGAGGGTCAGCCACTATTTGAACTCTCTCAACTTCTCGCTTATCGTAGAGGGCAATACGATCCTAGTCAACTTCTTGAAAAGATACCAGATTGTGGAATCCGCAAAATGATACTTCATATGATTCAGTTGGAACCGGAATTGCGACTTTCTGCTGATAGCTACCTGCAGGAGTATACAACTATTGTGTTTCCGAGTTACTTCTCTCCGTTTCTGCATAATTTTCATTGTTTCTGGAATCCGCTTCATTCGGATATGAGGGTTGCACTTTGCCAGAGTGTTTTTCCTGAGATACTGAAACAAATGATGAGCAATAGGACAACTCAGGACACTAGCACTGGACATGGAACTCCTTCAAACATCCATGCTGTTAGCGGTAAATCTTCTCAGGACATGATTGCAATGCAGAACATGAATTTGACAAAGGGCTCACTGGGAAAGAAAGTAGAGATGGATAAAGGTTTCAAGCGCGATCAATTTGAACTTCTCGGTGATATCAATACCCTTCTTAGGGATGTTAAACAAAGTAATCATTATTCTAATATGAAGCCAATGGTGGAGGACAACACTAATTCTGCGTTTTCCCAGAATCTTGGTAACTATGGCATGCAATCTCCTGGTGAATTACTTCAGAGTATCTCCCATGCATTTAGGAGAAATGATCATCCTTTTCTAAAAAAGATTACTATGAATGATTTGAATTCATTGATGTCCAAGTATGACAGCCAGTCAGATACCTTTGGTATGCCTTTTTTACCATTACCTGAAGACAGTTTGAGATGTGAGGGCATGGTTCTGATTACCTCTTTGCTATGTTCCTGCATACGCAATGTCAAATTGCCTCATCTAAGGAGGAGGGCGATACTTTTGCTGAAGTCATCTGCGTTGTATATTGATGATGAAAACAGGTTGCAGCGTGTAATTCCATATGTTGTTGCAATGCTTTCAGATCAAGCTGCAATTGTGCGTTGTGCAGCCTTGGAGACGTTATGTGACATTCTCCCTTTAGTTCGAGATTTTCCTCCTAGTGATGCAAAAATTTTTCCAGAGTATATTCTGCCAATGCTTTCCATGCTTCCTGATGATACAGAGGAAAGTGTGAGGATATGTTATGCCAGTAATATAGCTAAGCTGGCACTAACTGCTTATGGTTTTTTAGTTCACTCAATAACCTTGAGTGAGGCTGGTGTGCTTGATGAAGTAAGTTCTCCAGAGAAGCTACTGGCATCATCTAGTGAGACATCTGGTCAGCTCCACAAGGTAAATAATGATGCACAGCTTGCCCAGTTGAGGAAGTCCATAGCTGACGTCATTCAAGAGCTTGTTATGGGTCCCAAGCAAACTCCCAATATCAGGAGAGCACTCCTTCAGGATATCAGCAATCTATGCTGCTTCTTTGGCCAGAGGCAGAGTAATGACTTTTTGTTACCCATTCTCCCTGCTTTTCTGAATGATCGAGATGAGCAGCTAAGAGCAGTATTCTACGGGCAAATTGTGTATGTATGCTTCTTTGTGGGTCAAAGAAGTGTGGAAGAATATCTTTTACCTTATATTGAGCAAGCTGTAAGTGATGCTACAGAGGCTGTTATTGTCAATGCATTGGACTGTTTGGCCATTTTGTGCAAAAGTGGTTACCTGCGCAAGAGGATACTCCTGGAAATGATTGAGCGTTCATTTCCATTGTTATGCTATCCTAGTCAATGGGTAAGAAGGTCGGCTGTCAGTTTTATTGCAGCCAGCAGTGATTGCTTGGGTGCAGTAGATTCCTATGTATTCCTTGCTCCTGTTATTCGTCCCCTTCTTCGTAGACAGCCAGCATCTCTAGCTTCTGAGAAGGCTCTCCTTTCATGTTTGAAGCCTCCTGTCTCAAGACAGGTATTTTACCAAGTTCTAGAAAATGCAAGGAGTTCAGACATGTTGGAAAGACAGAGAAAGATATGGTACAATTCTTGGCCTCAATCTAAACAATGGGAAAGTGTGGATTTACTTCATAAAGGGGTTGTCGAATTGAATTCGATGAGGAGCTGGACAGACGACCAAGAAAGTCCCGAGGGTCAAAAACATTCTGGAAATGAATTGCAACAGGGAAAGCTAACTGAATGTGATGATGGTGAGGCTAAGTTTGGATGTATGGGAAGCTTTACGCGCAATGCTTCAAGCACAGTTGACATTCATGATCCTTTATCCTCTGAAAAGTTGCAGTATTCGGGCTTTATGTGGCCACAGGGAAGTACTGTGAATAGCTTCATGTGTGATAAATCATCAGTAGGGATACCTTTGTACTCATTTAGCATGGACAGGCGAGCAGTGGGAGTTCCTCCTGCTCCATCTGACTCTTCATTACAAGTGAGTTCTGTAGGAGTTGGTGCATCATCTATGCCATGGATGGATCGAGTAAATAAATCTTTTAGTTTAGCTAGTTCTGTTCCAGCACCCAAGCTCGTGTCAGGATCATTCAACATCAGCAGTGGTTCTAAACAATTCTACAGAGTAGTACATGAACCAGATGGTAGGGATAATGACCAAACAACCTTTGTCAATAGCAAGTTTCAAGATATGGGGTTATCTAGTGCAACAAAAGCAAGTTCTATTACTGTGGAAGATGCATCCTCCACAAGTGATCTAACAGGATTGCCCTCTTCTACAAGAGCTTCATCAATTCCAGATTCTGGTTGGAGGCCTCGTGGGGTTTTGGTTGCACACTTGCAAGAGCACCGGTCTGCTGTGAATGACATAGCCGTATCAACTGATCATAGCTTCTTTGTGAGTGCATCTGATGATTCCACTGTCAAGGTATGGGATTCGAGAAAGTTGGAAAAGGACATCTCATTTAGGTCAAGATTGACTTATCATCTGGAGGGAAGCCGTGCACTATGTTCTGTAATGCTCCGGGGCTGCGCTCAAGTTGTAGTTGGAGCATGTGATGGCATGATACATATGTTTTCTGTTGATTACATTTCCAGAGGTCTCGGTAATGTAGTTGAGAAGTATTCAGGTGTTGCTGATATCAAGAAGAAGGACACTAAAGAAGGGGCAATACTTAGCCTTTTGAATTTCTCTGCTGACAACTATGCAAATCAGATGGTTATGTACAGCACCCAAAATTGTGGGATCCATCTTTGGGATATAAGAACGAATTCAGATTCTTGGACATTGAAAGCAACTCCAGAGGAGGGCTATGTATCTTCCCTAGTAACAGGCCCTTGTGAAAATTGGTTTGTGTCAGGATCTTCAAGGGGTGTGCTCACTCTTTGGGATATGAGGTTCCTTATTCCTGTGAATACATGGCAATATTCTGCTGTTTGCCCCATAGAGAAAATGTGTCTTTTCCTTCCTCCTCCAAATGCTTCTGTTTCTGCTTCTGCAAGGCCCCTTGTTTATGTTGCTGCAGGTTGCAATGAAGTTTCTCTTTGGAATGCAGAAAATGGCACCTGCCACCAGGTGTTGAGGGTTGCGAGTTATGAAAGTGATGCTGAAATGTCTGAGGTGCCTTGGGCCTTGGCGAGATCATCAAATAAAAATTCCAAAGCGGATATGAGACGGAATGTCAATCCTCATTACAGAGTTGATGAACTGAATGAACCACCTCCTCGTATTCCTGGCATCCGTTCATTGCTTCCCTTACCTGGGGGTGATTTGTTAACTGGGGGAACCGACTTGAAGATACGTCGATGGGATCATTACAGCCCCGATCGAAGTTACTGTATTTGTGGGCCAAACTTGAAGGGTGTTGGAAATGATGATTTCTATGGAACACGGTCTAGCTTTGGGGTGCAAGTTGTGCAGGTATCATTTCTAGAAACTTTAAAGTTAGACGGGTTAGTACCTTCCTTGGAGGGTATGGTCATGTCCACATGTAGTGTATACATGCAGAAGATTTGGTTTGGGTGGAAAATTTCTGTTCTATTTGTTGCTTTGCTCCTGAAAACCAGAGATCAATCACTTGGTAGTTTCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000011 GO:0000151 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005737 GO:0005768 GO:0005770 GO:0005942 GO:0006355 GO:0006357 GO:0006464 GO:0006468 GO:0006605 GO:0006623 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006886 GO:0006914 GO:0006996 GO:0007033 GO:0007034 GO:0007154 GO:0007165 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009555 GO:0009846 GO:0009856 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010008 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0016236 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019219 GO:0019222 GO:0019538 GO:0019898 GO:0022414 GO:0023052 GO:0030242 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031410 GO:0031461 GO:0031967 GO:0031975 GO:0031982 GO:0032182 GO:0032501 GO:0032502 GO:0032507 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0033036 GO:0033365 GO:0034067 GO:0034243 GO:0034271 GO:0034272 GO:0034613 GO:0035032 GO:0035556 GO:0036211 GO:0042886 GO:0043130 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0044706 GO:0045053 GO:0045184 GO:0045185 GO:0045324 GO:0045893 GO:0045935 GO:0045944 GO:0046907 GO:0048015 GO:0048017 GO:0048229 GO:0048308 GO:0048518 GO:0048522 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051235 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051651 GO:0051704 GO:0051716 GO:0060255 GO:0061695 GO:0061919 GO:0065007 GO:0065008 GO:0070727 GO:0071561 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0080008 GO:0080090 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0120095 GO:0140096 GO:1901564 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04136 ko04138 ko04140 ko04371 map04136 map04138 map04140 map04371
Pfam Domains
Protein Families
HEAT Pkinase WD40

Protein Analysis

1345

Amino Acids

149.78

Weight (kDa)

6.14

Isoelectric Point (pI)

52.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VPS15-like_hel PF22956 293 - 656 1.5e-123 VPS15-like, helical domain
Beta-prop_WDR5 PF25175 922 - 1009 4e-08 WDR5 beta-propeller domain
WD40_Prp19 PF24814 922 - 1128 2.9e-11 Prp19 WD40 domain
WD40 PF00400 923 - 960 1.1e-08 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 926 - 1123 2.8e-10 THOC3 beta-propeller domain
WD40_Gbeta PF25391 928 - 1136 2.1e-07 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 932 - 1133 1.5e-07 WDHD1 first WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3518
AatII GACGTC 1 cut(s) 1389
Acc16I TGCGCA 1 cut(s) 1718
Acc36I ACCTGC 5 cut(s) 252, 1722, 3458, 3518, 3853
AccB1I GGYRCC 2 cut(s) 3506, 3565
AccB7I CCANNNNNTGG 2 cut(s) 173, 2747
AccI GTMKAC 3 cut(s) 254, 1885, 3942
AccII CGCG 2 cut(s) 549, 2224
AciI CCGC 4 cut(s) 181, 320, 453, 3608
AclWI GGATC 8 cut(s) 134, 2249, 2475, 2536, 3184, 3197, 3313, 3762
AcoI YGGCCR 3 cut(s) 1477, 1689, 2297
AcsI RAATTY 8 cut(s) 493, 778, 1116, 2089, 3130, 3214, 3598, 3972
AcuI CTGAAG 4 cut(s) 692, 864, 971, 1430
AcyI GRCGYC 1 cut(s) 1386
AdeI CACNNNGTG 1 cut(s) 3520
AfaI GTAC 6 cut(s) 2021, 2311, 2360, 2574, 3170, 3903
AflIII ACRYGT 2 cut(s) 1992, 3932
AgeI ACCGGT 1 cut(s) 2793
AhdI GACNNNNNGTC 3 cut(s) 805, 1323, 3623
AjnI CCWGG 5 cut(s) 694, 1731, 3516, 3676, 3703
AloI GAACNNNNNNTCC 2 cut(s) 3326, 3358
Alw21I GWGCWC 4 cut(s) 1441, 2793, 2950, 3324
Alw26I GTCTC 5 cut(s) 1055, 1310, 1948, 3050, 3610
Alw44I GTGCAC 1 cut(s) 2946
AlwI GGATC 8 cut(s) 134, 2249, 2475, 2536, 3184, 3197, 3313, 3762
AlwNI CAGNNNCTG 2 cut(s) 1661, 2747
Ama87I CYCGRG 1 cut(s) 2126
AoxI GGCC 9 cut(s) 1477, 1689, 2030, 2297, 2756, 3277, 3446, 3573, 3791
ApaLI GTGCAC 1 cut(s) 2946
ApeKI GCWGC 8 cut(s) 991, 1034, 1052, 1467, 1543, 1811, 2973, 3464
ApoI RAATTY 8 cut(s) 493, 778, 1116, 2089, 3130, 3214, 3598, 3972
AsiGI ACCGGT 1 cut(s) 2793
Asp700I GAANNNNTTC 4 cut(s) 430, 705, 2321, 3214
AspLEI GCGC 4 cut(s) 549, 1719, 2226, 2978
AspS9I GGNCC 5 cut(s) 1409, 3278, 3447, 3573, 3791
AsuC2I CCSGG 1 cut(s) 2969
AsuHPI GGTGA 4 cut(s) 581, 710, 2201, 3722
AvaI CYCGRG 1 cut(s) 2126
AvaII GGWCC 1 cut(s) 1409
BaeGI GKGCMC 1 cut(s) 2950
BaeI ACNNNNGTAYC 4 cut(s) 2342, 2375, 3002, 3035
BalI TGGCCA 3 cut(s) 1479, 1691, 2299
BamHI GGATCC 1 cut(s) 3189
BanI GGYRCC 2 cut(s) 3506, 3565
BanII GRGCYC 1 cut(s) 509
BarI GAAGNNNNNNTAC 4 cut(s) 1568, 1600, 3099, 3131
BauI CACGAG 2 cut(s) 2519, 2760
BbsI GAAGAC 1 cut(s) 852
Bbv12I GWGCWC 4 cut(s) 1441, 2793, 2950, 3324
BbvI GCAGC 8 cut(s) 1003, 1021, 1064, 1454, 1555, 1823, 2960, 3451
BceAI ACGGC 2 cut(s) 2801, 2928
BcgI CGANNNNNNTGC 2 cut(s) 928, 962
BciT130I CCWGG 5 cut(s) 696, 1733, 3518, 3678, 3705
BciVI GTATCC 2 cut(s) 1719, 2343
BclI TGATCA 2 cut(s) 739, 2827
BcnI CCSGG 1 cut(s) 2969
BcoDI GTCTC 5 cut(s) 1055, 1310, 1948, 3050, 3610
BfmI CTRYAG 6 cut(s) 51, 245, 1653, 2433, 2563, 3465
BfuAI ACCTGC 5 cut(s) 252, 1722, 3458, 3518, 3853
BfuI GTATCC 2 cut(s) 1719, 2343
BisI GCNGC 8 cut(s) 992, 1035, 1053, 1468, 1544, 1812, 2974, 3465
BlpI GCTNAGC 2 cut(s) 69, 1206
BlsI GCNGC 8 cut(s) 993, 1036, 1054, 1469, 1545, 1813, 2975, 3466
BmcAI AGTACT 1 cut(s) 2311
Bme1390I CCNGG 6 cut(s) 696, 1733, 2969, 3518, 3678, 3705
Bme18I GGWCC 1 cut(s) 1409
BmeRI GACNNNNNGTC 3 cut(s) 805, 1323, 3623
BmeT110I CYCGRG 1 cut(s) 2126
BmgT120I GGNCC 5 cut(s) 1409, 3278, 3447, 3573, 3791
BmiI GGNNCC 9 cut(s) 213, 1410, 1411, 3191, 3344, 3449, 3508, 3567, 3730
BmrFI CCNGG 6 cut(s) 696, 1733, 2969, 3518, 3678, 3705
BmrI ACTGGG 3 cut(s) 521, 1357, 3732
BmuI ACTGGG 3 cut(s) 521, 1357, 3732
BpiI GAAGAC 1 cut(s) 852
BpmI CTGGAG 3 cut(s) 1272, 2954, 3228
Bpu1102I GCTNAGC 2 cut(s) 69, 1206
BpuEI CTTGAG 4 cut(s) 101, 1273, 1930, 2964
BpuMI CCSGG 1 cut(s) 2969
BsaHI GRCGYC 1 cut(s) 1386
BsaI GGTCTC 1 cut(s) 3050
BsaJI CCNNGG 8 cut(s) 1056, 2127, 2459, 2968, 3569, 3576, 3704, 3909
BsaWI WCCGGW 2 cut(s) 214, 2793
BsaXI ACNNNNNCTCC 4 cut(s) 1497, 1527, 2746, 2776
Bse118I RCCGGY 1 cut(s) 2793
Bse1I ACTGG 7 cut(s) 421, 516, 805, 1194, 1305, 1363, 3727
Bse3DI GCAATG 6 cut(s) 486, 913, 1023, 2233, 3481, 3689
BseBI CCWGG 5 cut(s) 696, 1733, 3518, 3678, 3705
BseDI CCNNGG 8 cut(s) 1056, 2127, 2459, 2968, 3569, 3576, 3704, 3909
BseGI GGATG 7 cut(s) 438, 598, 742, 2210, 2469, 2684, 3681
BseMI GCAATG 6 cut(s) 486, 913, 1023, 2233, 3481, 3689
BseMII CTCAG 6 cut(s) 60, 357, 416, 479, 1899, 3552
BseNI ACTGG 7 cut(s) 421, 516, 805, 1194, 1305, 1363, 3727
BseRI GAGGAG 5 cut(s) 946, 2113, 3263, 3405, 3657
BseSI GKGCMC 1 cut(s) 2950
BseXI GCAGC 8 cut(s) 1003, 1021, 1064, 1454, 1555, 1823, 2960, 3451
BsgI GTGCAG 3 cut(s) 1071, 1854, 3881
Bsh1236I CGCG 2 cut(s) 549, 2224
Bsh1285I CGRYCG 1 cut(s) 3772
BshFI GGCC 9 cut(s) 1479, 1691, 2032, 2299, 2758, 3279, 3448, 3575, 3793
BshNI GGYRCC 2 cut(s) 3506, 3565
BshTI ACCGGT 1 cut(s) 2793
BsiEI CGRYCG 1 cut(s) 3772
BsiHKAI GWGCWC 4 cut(s) 1441, 2793, 2950, 3324
BsiHKCI CYCGRG 1 cut(s) 2126
BsiSI CCGG 3 cut(s) 215, 2794, 2968
BslFI GGGAC 3 cut(s) 1395, 1857, 2109
BsmAI GTCTC 5 cut(s) 1055, 1310, 1948, 3050, 3610
BsmBI CGTCTC 2 cut(s) 1055, 3610
BsmFI GGGAC 3 cut(s) 1395, 1857, 2109
BsmI GAATGC 1 cut(s) 3499
BsnI GGCC 9 cut(s) 1479, 1691, 2032, 2299, 2758, 3279, 3448, 3575, 3793
Bso31I GGTCTC 1 cut(s) 3050
BsoBI CYCGRG 1 cut(s) 2126
Bsp1286I GDGCHC 5 cut(s) 509, 1441, 2793, 2950, 3324
Bsp1407I TGTACA 1 cut(s) 3168
Bsp1720I GCTNAGC 2 cut(s) 69, 1206
Bsp19I CCATGG 1 cut(s) 2459
BspACI CCGC 4 cut(s) 181, 320, 453, 3608
BspANI GGCC 9 cut(s) 1479, 1691, 2032, 2299, 2758, 3279, 3448, 3575, 3793
BspCNI CTCAG 6 cut(s) 61, 358, 415, 478, 1900, 3553
BspFNI CGCG 2 cut(s) 549, 2224
BspHI TCATGA 1 cut(s) 2251
BspLI GGNNCC 9 cut(s) 213, 1410, 1411, 3191, 3344, 3449, 3508, 3567, 3730
BspMAI CTGCAG 2 cut(s) 249, 3469
BspMI ACCTGC 5 cut(s) 252, 1722, 3458, 3518, 3853
BspPI GGATC 8 cut(s) 134, 2249, 2475, 2536, 3184, 3197, 3313, 3762
BspT107I GGYRCC 2 cut(s) 3506, 3565
BspTNI GGTCTC 1 cut(s) 3050
BsrDI GCAATG 6 cut(s) 486, 913, 1023, 2233, 3481, 3689
BsrFI RCCGGY 1 cut(s) 2793
BsrGI TGTACA 1 cut(s) 3168
BsrI ACTGG 7 cut(s) 421, 516, 805, 1194, 1305, 1363, 3727
BssAI RCCGGY 1 cut(s) 2793
BssECI CCNNGG 8 cut(s) 1056, 2127, 2459, 2968, 3569, 3576, 3704, 3909
BssNAI GTATAC 2 cut(s) 255, 3943
BssNI GRCGYC 1 cut(s) 1386
BssSI CACGAG 2 cut(s) 2519, 2760
BssT1I CCWWGG 5 cut(s) 1056, 2459, 3569, 3576, 3909
Bst1107I GTATAC 2 cut(s) 255, 3943
Bst2BI CACGAG 2 cut(s) 2519, 2760
Bst2UI CCWGG 5 cut(s) 696, 1733, 3518, 3678, 3705
Bst4CI ACNGT 8 cut(s) 851, 1685, 2242, 2314, 2674, 2866, 3782, 3837
Bst6I CTCTTC 2 cut(s) 2419, 2722
BstACI GRCGYC 1 cut(s) 1386
BstAPI GCANNNNNTGC 6 cut(s) 17, 1220, 1358, 1464, 1824, 3859
BstAUI TGTACA 1 cut(s) 3168
BstC8I GCNNGC 7 cut(s) 686, 1212, 1359, 1638, 1816, 1893, 2382
BstDSI CCRYGG 1 cut(s) 2459
BstEII GGTNACC 1 cut(s) 1709
BstF5I GGATG 7 cut(s) 438, 598, 742, 2210, 2469, 2684, 3681
BstFNI CGCG 2 cut(s) 549, 2224
BstHHI GCGC 4 cut(s) 549, 1719, 2226, 2978
BstMAI GTCTC 5 cut(s) 1055, 1310, 1948, 3050, 3610
BstMCI CGRYCG 1 cut(s) 3772
BstNI CCWGG 5 cut(s) 696, 1733, 3518, 3678, 3705
BstNSI RCATGY 5 cut(s) 688, 1996, 3000, 3936, 3949
BstPI GGTNACC 1 cut(s) 1709
BstSCI CCNGG 6 cut(s) 694, 1731, 2967, 3516, 3676, 3703
BstSFI CTRYAG 6 cut(s) 51, 245, 1653, 2433, 2563, 3465
BstSLI GKGCMC 1 cut(s) 2950
BstUI CGCG 2 cut(s) 549, 2224
BstV1I GCAGC 8 cut(s) 1003, 1021, 1064, 1454, 1555, 1823, 2960, 3451
BstV2I GAAGAC 1 cut(s) 852
BstX2I RGATCY 2 cut(s) 3189, 3305
BstXI CCANNNNNNTGG 2 cut(s) 671, 3185
BstYI RGATCY 2 cut(s) 3189, 3305
BstZ17I GTATAC 2 cut(s) 255, 3943
BsuI GTATCC 2 cut(s) 1719, 2343
BsuRI GGCC 9 cut(s) 1479, 1691, 2032, 2299, 2758, 3279, 3448, 3575, 3793
BtgI CCRYGG 1 cut(s) 2459
BtsCI GGATG 7 cut(s) 438, 598, 742, 2210, 2469, 2684, 3681
BtsI GCAGTG 3 cut(s) 1825, 2391, 2551
BtsIMutI CAGTG 6 cut(s) 414, 509, 1825, 2391, 2551, 2862
BveI ACCTGC 5 cut(s) 252, 1722, 3458, 3518, 3853
Cac8I GCNNGC 7 cut(s) 686, 1212, 1359, 1638, 1816, 1893, 2382
CaiI CAGNNNCTG 2 cut(s) 1661, 2747
CciI TCATGA 1 cut(s) 2251
CfoI GCGC 4 cut(s) 549, 1719, 2226, 2978
Cfr10I RCCGGY 1 cut(s) 2793
Cfr13I GGNCC 5 cut(s) 1409, 3278, 3447, 3573, 3791
CsiI ACCWGGT 1 cut(s) 3516
Csp6I GTAC 6 cut(s) 2020, 2310, 2359, 2573, 3169, 3902
CspAI ACCGGT 1 cut(s) 2793
CviQI GTAC 6 cut(s) 2020, 2310, 2359, 2573, 3169, 3902
DraI TTTAAA 1 cut(s) 3885
DraIII CACNNNGTG 1 cut(s) 3520
DriI GACNNNNNGTC 3 cut(s) 805, 1323, 3623
EaeI YGGCCR 3 cut(s) 1477, 1689, 2297
Eam1104I CTCTTC 2 cut(s) 2419, 2722
Eam1105I GACNNNNNGTC 3 cut(s) 805, 1323, 3623
EarI CTCTTC 2 cut(s) 2419, 2722
Eco130I CCWWGG 5 cut(s) 1056, 2459, 3569, 3576, 3909
Eco147I AGGCCT 1 cut(s) 2758
Eco24I GRGCYC 1 cut(s) 509
Eco31I GGTCTC 1 cut(s) 3050
Eco32I GATATC 4 cut(s) 43, 574, 1453, 3085
Eco47I GGWCC 1 cut(s) 1409
Eco57I CTGAAG 4 cut(s) 692, 864, 971, 1430
Eco88I CYCGRG 1 cut(s) 2126
Eco91I GGTNACC 1 cut(s) 1709
EcoO109I RGGNCCY 3 cut(s) 1409, 3278, 3447
EcoO65I GGTNACC 1 cut(s) 1709
EcoRI GAATTC 3 cut(s) 778, 2089, 3214
EcoRII CCWGG 5 cut(s) 694, 1731, 3516, 3676, 3703
EcoRV GATATC 4 cut(s) 43, 574, 1453, 3085
EcoT14I CCWWGG 5 cut(s) 1056, 2459, 3569, 3576, 3909
EcoT22I ATGCAT 3 cut(s) 727, 1678, 2686
EcoT38I GRGCYC 1 cut(s) 509
ErhI CCWWGG 5 cut(s) 1056, 2459, 3569, 3576, 3909
Esp3I CGTCTC 2 cut(s) 1055, 3610
FalI AAGNNNNNCTT 6 cut(s) 1602, 1634, 2908, 2940, 3471, 3503
FaqI GGGAC 3 cut(s) 1395, 1857, 2109
FauNDI CATATG 3 cut(s) 198, 1006, 3015
FbaI TGATCA 2 cut(s) 739, 2827
FblI GTMKAC 3 cut(s) 254, 1885, 3942
Fnu4HI GCNGC 8 cut(s) 992, 1035, 1053, 1468, 1544, 1812, 2974, 3465
FokI GGATG 7 cut(s) 425, 605, 729, 2217, 2476, 2671, 3668
FriOI GRGCYC 1 cut(s) 509
Fsp4HI GCNGC 8 cut(s) 992, 1035, 1053, 1468, 1544, 1812, 2974, 3465
FspI TGCGCA 1 cut(s) 1718
GlaI GCGC 4 cut(s) 548, 1718, 2225, 2977
GluI GCNGC 8 cut(s) 992, 1035, 1053, 1468, 1544, 1812, 2974, 3465
GsuI CTGGAG 3 cut(s) 1272, 2954, 3228
HaeIII GGCC 9 cut(s) 1479, 1691, 2032, 2299, 2758, 3279, 3448, 3575, 3793
HapII CCGG 3 cut(s) 215, 2794, 2968
HhaI GCGC 4 cut(s) 549, 1719, 2226, 2978
Hin1I GRCGYC 1 cut(s) 1386
Hin6I GCGC 4 cut(s) 547, 1717, 2224, 2976
HinP1I GCGC 4 cut(s) 547, 1717, 2224, 2976
HincII GTYRAC 3 cut(s) 149, 2245, 3720
HindII GTYRAC 3 cut(s) 149, 2245, 3720
HindIII AAGCTT 1 cut(s) 2215
HpaI GTTAAC 1 cut(s) 3720
HpaII CCGG 3 cut(s) 215, 2794, 2968
HphI GGTGA 4 cut(s) 581, 710, 2201, 3722
Hpy166II GTNNAC 9 cut(s) 149, 255, 1240, 1886, 2245, 2948, 3720, 3930, 3943
Hpy8I GTNNAC 9 cut(s) 149, 255, 1240, 1886, 2245, 2948, 3720, 3930, 3943
Hpy99I CGWCG 1 cut(s) 3750
HpyCH4III ACNGT 8 cut(s) 851, 1685, 2242, 2314, 2674, 2866, 3782, 3837
HpyCH4IV ACGT 3 cut(s) 1064, 1386, 3745
HpySE526I ACGT 3 cut(s) 1064, 1386, 3745
Hsp92I GRCGYC 1 cut(s) 1386
HspAI GCGC 4 cut(s) 547, 1717, 2224, 2976
KflI GGGWCCC 1 cut(s) 1409
Ksp22I TGATCA 2 cut(s) 739, 2827
KspAI GTTAAC 1 cut(s) 3720
LmnI GCTCC 7 cut(s) 1335, 1864, 2100, 2407, 2970, 2993, 4002
Lsp1109I GCAGC 8 cut(s) 1003, 1021, 1064, 1454, 1555, 1823, 2960, 3451
MabI ACCWGGT 1 cut(s) 3516
MaeII ACGT 3 cut(s) 1064, 1386, 3745
MaeIII GTNAC 7 cut(s) 275, 674, 1070, 1503, 1709, 3271, 3776
MfeI CAATTG 1 cut(s) 1038
MflI RGATCY 2 cut(s) 3189, 3305
MhlI GDGCHC 5 cut(s) 509, 1441, 2793, 2950, 3324
MlsI TGGCCA 3 cut(s) 1479, 1691, 2299
MluNI TGGCCA 3 cut(s) 1479, 1691, 2299
MlyI GAGTC 1 cut(s) 2405
MmeI TCCRAC 6 cut(s) 189, 1977, 2732, 2871, 2971, 3790
Mox20I TGGCCA 3 cut(s) 1479, 1691, 2299
Mph1103I ATGCAT 3 cut(s) 727, 1678, 2686
MroXI GAANNNNTTC 4 cut(s) 430, 705, 2321, 3214
MscI TGGCCA 3 cut(s) 1479, 1691, 2299
MseI TTAA 4 cut(s) 26, 597, 3719, 3884
MslI CAYNNNNRTG 1 cut(s) 1762
Msp20I TGGCCA 3 cut(s) 1479, 1691, 2299
MspI CCGG 3 cut(s) 215, 2794, 2968
MspR9I CCNGG 6 cut(s) 696, 1733, 2969, 3518, 3678, 3705
MunI CAATTG 1 cut(s) 1038
Mva1269I GAATGC 1 cut(s) 3499
MvaI CCWGG 5 cut(s) 696, 1733, 3518, 3678, 3705
MvnI CGCG 2 cut(s) 549, 2224
NciI CCSGG 1 cut(s) 2969
NcoI CCATGG 1 cut(s) 2459
NdeI CATATG 3 cut(s) 198, 1006, 3015
NlaIV GGNNCC 9 cut(s) 213, 1410, 1411, 3191, 3344, 3449, 3508, 3567, 3730
NmuCI GTSAC 1 cut(s) 1070
NsbI TGCGCA 1 cut(s) 1718
NsiI ATGCAT 3 cut(s) 727, 1678, 2686
NspI RCATGY 5 cut(s) 688, 1996, 3000, 3936, 3949
PaeI GCATGC 1 cut(s) 688
PagI TCATGA 1 cut(s) 2251
PaqCI CACCTGC 1 cut(s) 3518
PceI AGGCCT 1 cut(s) 2758
PciI ACATGT 2 cut(s) 1992, 3932
PctI GAATGC 1 cut(s) 3499
PdmI GAANNNNTTC 4 cut(s) 430, 705, 2321, 3214
PfeI GAWTC 9 cut(s) 177, 202, 316, 667, 1841, 2744, 2858, 2879, 3221
PflMI CCANNNNNTGG 2 cut(s) 173, 2747
PfoI TCCNGGA 1 cut(s) 1731
PinAI ACCGGT 1 cut(s) 2793
PkrI GCNGC 8 cut(s) 993, 1036, 1054, 1469, 1545, 1813, 2975, 3466
Ple19I CGATCG 1 cut(s) 3772
PleI GAGTC 1 cut(s) 2405
PpsI GAGTC 1 cut(s) 2405
PpuMI RGGWCCY 1 cut(s) 1409
PscI ACATGT 2 cut(s) 1992, 3932
Psp5II RGGWCCY 1 cut(s) 1409
Psp6I CCWGG 5 cut(s) 694, 1731, 3516, 3676, 3703
PspEI GGTNACC 1 cut(s) 1709
PspGI CCWGG 5 cut(s) 694, 1731, 3516, 3676, 3703
PspN4I GGNNCC 9 cut(s) 213, 1410, 1411, 3191, 3344, 3449, 3508, 3567, 3730
PspPI GGNCC 5 cut(s) 1409, 3278, 3447, 3573, 3791
PspPPI RGGWCCY 1 cut(s) 1409
PstI CTGCAG 2 cut(s) 249, 3469
PstNI CAGNNNCTG 2 cut(s) 1661, 2747
PsuI RGATCY 2 cut(s) 3189, 3305
PvuI CGATCG 1 cut(s) 3772
RsaI GTAC 6 cut(s) 2021, 2311, 2360, 2574, 3170, 3903
RsaNI GTAC 6 cut(s) 2020, 2310, 2359, 2573, 3169, 3902
RseI CAYNNNNRTG 1 cut(s) 1762
SaqAI TTAA 4 cut(s) 26, 597, 3719, 3884
SatI GCNGC 8 cut(s) 992, 1035, 1053, 1468, 1544, 1812, 2974, 3465
Sau96I GGNCC 5 cut(s) 1409, 3278, 3447, 3573, 3791
SbfI CCTGCAGG 1 cut(s) 249
ScaI AGTACT 1 cut(s) 2311
SchI GAGTC 1 cut(s) 2405
ScrFI CCNGG 6 cut(s) 696, 1733, 2969, 3518, 3678, 3705
SdaI CCTGCAGG 1 cut(s) 249
SduI GDGCHC 5 cut(s) 509, 1441, 2793, 2950, 3324
SexAI ACCWGGT 1 cut(s) 3516
SfcI CTRYAG 6 cut(s) 51, 245, 1653, 2433, 2563, 3465
SinI GGWCC 1 cut(s) 1409
SmiMI CAYNNNNRTG 1 cut(s) 1762
SmlI CTYRAG 4 cut(s) 80, 1252, 1945, 2979
SmoI CTYRAG 4 cut(s) 80, 1252, 1945, 2979
SphI GCATGC 1 cut(s) 688
Sse8387I CCTGCAGG 1 cut(s) 249
SseBI AGGCCT 1 cut(s) 2758
SsiI CCGC 4 cut(s) 181, 320, 453, 3608
SspI AATATT 1 cut(s) 3371
StuI AGGCCT 1 cut(s) 2758
StyD4I CCNGG 6 cut(s) 694, 1731, 2967, 3516, 3676, 3703
StyI CCWWGG 5 cut(s) 1056, 2459, 3569, 3576, 3909
TaaI ACNGT 8 cut(s) 851, 1685, 2242, 2314, 2674, 2866, 3782, 3837
TaiI ACGT 3 cut(s) 1067, 1389, 3748
TaqI TCGA 8 cut(s) 1090, 1534, 2082, 2093, 2470, 2882, 3748, 3772
TatI WGTACW 4 cut(s) 2309, 2358, 2572, 3168
TfiI GAWTC 9 cut(s) 177, 202, 316, 667, 1841, 2744, 2858, 2879, 3221
Tru1I TTAA 4 cut(s) 26, 597, 3719, 3884
Tru9I TTAA 4 cut(s) 26, 597, 3719, 3884
TscAI CASTG 6 cut(s) 421, 516, 1825, 2391, 2551, 2869
TseFI GTSAC 1 cut(s) 1070
TseI GCWGC 8 cut(s) 991, 1034, 1052, 1467, 1543, 1811, 2973, 3464
Tsp45I GTSAC 1 cut(s) 1070
TspGWI ACGGA 3 cut(s) 276, 3634, 3674
TspRI CASTG 6 cut(s) 421, 516, 1825, 2391, 2551, 2869
Van91I CCANNNNNTGG 2 cut(s) 173, 2747
VneI GTGCAC 1 cut(s) 2946
VpaK11BI GGWCC 1 cut(s) 1409
XapI RAATTY 8 cut(s) 493, 778, 1116, 2089, 3130, 3214, 3598, 3972
XbaI TCTAGA 2 cut(s) 1969, 3874
XceI RCATGY 5 cut(s) 688, 1996, 3000, 3936, 3949
XcmI CCANNNNNNNNNTGG 1 cut(s) 2748
XmiI GTMKAC 3 cut(s) 254, 1885, 3942
XmnI GAANNNNTTC 4 cut(s) 430, 705, 2321, 3214
ZraI GACGTC 1 cut(s) 1387
ZrmI AGTACT 1 cut(s) 2311
Zsp2I ATGCAT 3 cut(s) 727, 1678, 2686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.