Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0001360

Physical Location & Seq

Genomic Coordinates Reverse (-)

19247577 .. 19249019

Gene Structure 1443 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1085900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1443 bp

ATGATTCTTTCCAGTCCAATCTTTCTTGTAGTTGTAACAATCTGTATATTTGTAGCAATCCGCGTGGCGCTGTACAGAACTGGGCTGGTTTACATCGTGAAAAAATGGTGGCGGCGGTTCGAGGATTGCTTCCATGTCTACCAATCGTTCAAAGTCCCCGAATACAACGAGAGCATGCAGGAGAATCAGCTCTACGGCCGAGTCACCGACTATCTCAACTCGCTCACCTCTGTTGAAGATTCAGACTTCACCAATCTCGTCACCGGAAAAAAACCCAACGAAATTGTTCTCCAGCTCGACCCCAATCAGATCATCGAGGACAATTTCCTCGGTGCTAAGGTGATTTGGCAAGCAGAGGGCGGCGGCAGGAGTAGGAACTTGGTGTTGAAGGTTCGGAAAGCTGATAAGCGGCGGATTCTACGGCCGTATTTGCAGCACATCCACGTCGTCGCTGACGAGCTCGAGCAGAAGAAACGGGAGTTGAAGCTTTACATGAACGTCGACGCGCCGGACAGAGCGTGGAAGCCAGTCCCCTTCACTCACCCGTCGACCTTCGAGACCATATCAATGGAGTCCGATCTCAAATCGAAAGTCAAATCAGATTTGGAGTCATTTCTCAAGGCGAAACAGTACTATCACCGACTAGGTCGGGTCTGGAAACGGAGCTTTCTGCTATACGGGCCGTCGGGTACAGGAAAATCGAGCTTCGTCGCCGCCATGGCCAATTTCCTCGGGTACGACGTGTACGATCTGGACCTCTCGCGGGTCAAAGACGATTCGGAGCTGAAGATTCTTCTGTTGCAGACGACGAAAAAATCGGTCATTGTAATCGAGGATTTGGACCGATATTTAGCCGAGAAACCCGCGGGTCTGAGCTTTTCGGGTATATCGAATTTCATGGACGGGCTGCTGAATTCGTGCTGCGCCGAGGAGAGAGTCATGGTGTTCACGATGAATTCGAAGGACCACGTTGACCCGGCCTTCCTCCGACCGGGTCGGATCGACGTCCACATCCACTTTCCGCTCTGCGATTTCGGAGCATTCAAACATCTCGCGACGAGTTATCTCGGGGTGAAGGAGCACAAGCTCTTCTCGCAGGTGGAGGAGATTTTCCTGAGCGGGTCGAGTTTGAGTCCGGCAGAGATCGGCGAGTTGATGATCGCGAACCGGAACTCGCCGAGTCGGGCGATTAAATCGGTCATCACGGCTCTCCAGACGGACGGCGATAGGAGGGGCGCGGGATTGTGCGGAAGGCGGGTTGGGTTTGATGTCGGGTCGAGAAAGTGCGAATCGGGTGAACCCGGCGGGATGTTTTGTGGGGAAGGCGGTGTTCCTGCGGTTAGGGATATTAGGAAGTTGTACGGGTTCTTGAGGATGAAAAGCAGCAGGCACTCCTCTCATTCTCAGTCGTTCGATGAGGGTTCGCCGCACACCGAAGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0006996 GO:0007005 GO:0008150 GO:0009987 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017004 GO:0017062 GO:0017111 GO:0019866 GO:0022607 GO:0031090 GO:0031966 GO:0031967 GO:0031975 GO:0033108 GO:0034551 GO:0034622 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0065003 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA AAA_assoc

Protein Analysis

481

Amino Acids

54.25

Weight (kDa)

8.8

Isoelectric Point (pI)

46.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA_assoc	PF14363	31 - 118	3.4e-12	Domain associated at C-terminal with AAA
AAA	PF00004	224 - 342	7.3e-15	ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540	PF25568	343 - 406	2.4e-11	At3g28540-like, AAA+ ATPase lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001360)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46620
fragaria_vesca	FvH4_7g17220 FvH4_7g32500
malus_domestica	MD01G1085900.v1.1 MD01G1225700.v1.1 MD02G1229200.v1.1 MD07G1155000.v1.1 MD07G1296200.v1.1
prunus_persica	Prupe.2G193700_v2.0.a1 Prupe.2G315800_v2.0.a1
pyrus_communis	pycom01g23420 pycom07g15210 pycom07g26900
rosa_chinensis	RchiOBHm_Chr1g0360611 RchiOBHm_Chr1g0382341 RchiOBHm_Chr2g0086831 RchiOBHm_Chr2g0139501 RchiOBHm_Chr3g0458211 RchiOBHm_Chr3g0478421 RchiOBHm_Chr4g0427361
rosa_laevigata	RLG00000002572 RLG00000009827 RLG00000026185 RLG00000027795 RLG00000030781
rosa_multiflora	Rmu_sc0000110.1_g000040 Rmu_sc0001436.1_g000033 Rmu_sc0002188.1_g000003 Rmu_sc0002535.1_g000007 Rmu_sc0003020.1_g000016 Rmu_sc0003481.1_g000013 Rmu_sc0004697.1_g000002 Rmu_sc0007558.1_g000001 Rmu_sc0010192.1_g000001 Rmu_sc0015587.1_g000001 Rmu_sc0042341.1_g000002
rosa_roxburghii	Rroxscaffold_2G00093530 Rroxscaffold_4G00278180
rosa_rugosa	Rorug01G0280000 Rorug01G0433500 Rorug02G0149500 Rorug05G0571400
rosa_samantha	Rh1AG292200 Rh1BG257100 Rh1BG414500 Rh1CG274900 Rh1DG287000 Rh1DG446600 Rh5BG487600 Rh5CG511800
rosa_wichuraiana	Rw1G025870 Rw1G041280 Rw5G043520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1087
AatII	GACGTC	1 cut(s)	1008
Acc36I	ACCTGC	1 cut(s)	1087
AccBSI	CCGCTC	2 cut(s)	1024, 1119
AccI	GTMKAC	3 cut(s)	138, 501, 548
AccII	CGCG	7 cut(s)	63, 506, 763, 866, 1055, 1163, 1238
AclWI	GGATC	1 cut(s)	1007
AcoI	YGGCCR	3 cut(s)	196, 422, 720
AcsI	RAATTY	3 cut(s)	892, 913, 955
AcuI	CTGAAG	1 cut(s)	806
AcyI	GRCGYC	1 cut(s)	1005
AfaI	GTAC	6 cut(s)	74, 632, 691, 737, 746, 1361
AfiI	CCNNNNNNNGG	2 cut(s)	763, 991
AflIII	ACRYGT	1 cut(s)	741
AgsI	TTSAA	5 cut(s)	151, 236, 388, 484, 1045
AjiI	CACGTC	2 cut(s)	445, 742
Alw21I	GWGCWC	2 cut(s)	462, 1083
Alw26I	GTCTC	1 cut(s)	551
AlwI	GGATC	1 cut(s)	1007
Ama87I	CYCGRG	3 cut(s)	461, 731, 1067
AoxI	GGCC	5 cut(s)	196, 422, 680, 720, 978
ApeKI	GCWGC	4 cut(s)	433, 907, 921, 1383
ApoI	RAATTY	3 cut(s)	892, 913, 955
ArsI	GACNNNNNNTTYG	2 cut(s)	576, 608
Asp700I	GAANNNNTTC	1 cut(s)	285
AspLEI	GCGC	4 cut(s)	70, 508, 926, 1238
AspS9I	GGNCC	4 cut(s)	680, 754, 841, 964
AsuC2I	CCSGG	3 cut(s)	977, 993, 1302
AsuHPI	GGTGA	9 cut(s)	196, 217, 241, 253, 352, 533, 629, 1084, 1307
AsuII	TTCGAA	1 cut(s)	959
AvaI	CYCGRG	3 cut(s)	461, 731, 1067
AvaII	GGWCC	3 cut(s)	754, 841, 964
BalI	TGGCCA	1 cut(s)	722
BanII	GRGCYC	1 cut(s)	462
Bbv12I	GWGCWC	2 cut(s)	462, 1083
BbvI	GCAGC	4 cut(s)	445, 894, 908, 1395
BceAI	ACGGC	6 cut(s)	211, 409, 437, 667, 1221, 1237
BcnI	CCSGG	3 cut(s)	977, 993, 1302
BcoDI	GTCTC	1 cut(s)	551
BfaI	CTAG	1 cut(s)	644
BfoI	RGCGCY	1 cut(s)	71
BfuAI	ACCTGC	1 cut(s)	1087
BglI	GCCNNNNNGGC	1 cut(s)	719
BmcAI	AGTACT	1 cut(s)	632
Bme1390I	CCNGG	3 cut(s)	977, 993, 1302
Bme18I	GGWCC	3 cut(s)	754, 841, 964
BmeT110I	CYCGRG	3 cut(s)	461, 731, 1067
BmgBI	CACGTC	2 cut(s)	445, 742
BmgT120I	GGNCC	4 cut(s)	680, 754, 841, 964
BmrFI	CCNGG	3 cut(s)	977, 993, 1302
BmrI	ACTGGG	1 cut(s)	90
BmuI	ACTGGG	1 cut(s)	90
BpmI	CTGGAG	2 cut(s)	275, 1196
Bpu10I	CCTNAGC	2 cut(s)	336, 1115
Bpu14I	TTCGAA	1 cut(s)	959
BpuEI	CTTGAG	2 cut(s)	602, 1390
BpuMI	CCSGG	3 cut(s)	977, 993, 1302
BsaHI	GRCGYC	1 cut(s)	1005
BsaI	GGTCTC	1 cut(s)	551
BsaJI	CCNNGG	5 cut(s)	328, 717, 730, 864, 927
BsaWI	WCCGGW	2 cut(s)	263, 1167
Bsc4I	CCNNNNNNNGG	2 cut(s)	763, 991
Bse1I	ACTGG	3 cut(s)	12, 85, 527
BseDI	CCNNGG	5 cut(s)	328, 717, 730, 864, 927
BseGI	GGATG	4 cut(s)	438, 1011, 1314, 1380
BseLI	CCNNNNNNNGG	2 cut(s)	763, 991
BseMII	CTCAG	3 cut(s)	863, 1106, 1418
BseNI	ACTGG	3 cut(s)	12, 85, 527
BseRI	GAGGAG	3 cut(s)	944, 1118, 1384
BseX3I	CGGCCG	2 cut(s)	196, 422
BseXI	GCAGC	4 cut(s)	445, 894, 908, 1395
Bsh1236I	CGCG	7 cut(s)	63, 506, 763, 866, 1055, 1163, 1238
Bsh1285I	CGRYCG	3 cut(s)	199, 425, 992
BshFI	GGCC	5 cut(s)	198, 424, 682, 722, 980
BsiEI	CGRYCG	3 cut(s)	199, 425, 992
BsiHKAI	GWGCWC	2 cut(s)	462, 1083
BsiHKCI	CYCGRG	3 cut(s)	461, 731, 1067
BsiSI	CCGG	7 cut(s)	264, 509, 977, 992, 1136, 1168, 1302
BslFI	GGGAC	2 cut(s)	140, 515
BslI	CCNNNNNNNGG	2 cut(s)	763, 991
BsmAI	GTCTC	1 cut(s)	551
BsmFI	GGGAC	2 cut(s)	140, 515
BsmI	GAATGC	1 cut(s)	1040
BsnI	GGCC	5 cut(s)	198, 424, 682, 722, 980
Bso31I	GGTCTC	1 cut(s)	551
BsoBI	CYCGRG	3 cut(s)	461, 731, 1067
Bsp119I	TTCGAA	1 cut(s)	959
Bsp1286I	GDGCHC	2 cut(s)	462, 1083
Bsp1407I	TGTACA	1 cut(s)	72
Bsp143I	GATC	6 cut(s)	309, 577, 748, 999, 1143, 1158
Bsp19I	CCATGG	1 cut(s)	717
Bsp68I	TCGCGA	2 cut(s)	1055, 1163
BspANI	GGCC	5 cut(s)	198, 424, 682, 722, 980
BspCNI	CTCAG	3 cut(s)	864, 1107, 1417
BspFNI	CGCG	7 cut(s)	63, 506, 763, 866, 1055, 1163, 1238
BspMI	ACCTGC	1 cut(s)	1087
BspPI	GGATC	1 cut(s)	1007
BspQI	GCTCTTC	1 cut(s)	1094
BspT104I	TTCGAA	1 cut(s)	959
BspTNI	GGTCTC	1 cut(s)	551
BsrBI	CCGCTC	2 cut(s)	1024, 1119
BsrGI	TGTACA	1 cut(s)	72
BsrI	ACTGG	3 cut(s)	12, 85, 527
BssECI	CCNNGG	5 cut(s)	328, 717, 730, 864, 927
BssMI	GATC	6 cut(s)	309, 577, 748, 999, 1143, 1158
BssNI	GRCGYC	1 cut(s)	1005
BssT1I	CCWWGG	1 cut(s)	717
Bst4CI	ACNGT	1 cut(s)	630
Bst6I	CTCTTC	1 cut(s)	1094
BstACI	GRCGYC	1 cut(s)	1005
BstAUI	TGTACA	1 cut(s)	72
BstBI	TTCGAA	1 cut(s)	959
BstC8I	GCNNGC	3 cut(s)	176, 351, 1388
BstDEI	CTNAG	4 cut(s)	336, 872, 1115, 1404
BstDSI	CCRYGG	2 cut(s)	717, 864
BstF5I	GGATG	4 cut(s)	438, 1011, 1314, 1380
BstFNI	CGCG	7 cut(s)	63, 506, 763, 866, 1055, 1163, 1238
BstH2I	RGCGCY	1 cut(s)	71
BstHHI	GCGC	4 cut(s)	70, 508, 926, 1238
BstKTI	GATC	6 cut(s)	312, 580, 751, 1002, 1146, 1161
BstMAI	GTCTC	1 cut(s)	551
BstMBI	GATC	6 cut(s)	309, 577, 748, 999, 1143, 1158
BstMCI	CGRYCG	3 cut(s)	199, 425, 992
BstMWI	GCNNNNNNNGC	4 cut(s)	430, 679, 719, 1093
BstNSI	RCATGY	1 cut(s)	178
BstSCI	CCNGG	3 cut(s)	975, 991, 1300
BstUI	CGCG	7 cut(s)	63, 506, 763, 866, 1055, 1163, 1238
BstV1I	GCAGC	4 cut(s)	445, 894, 908, 1395
BstXI	CCANNNNNNTGG	1 cut(s)	568
BstZI	CGGCCG	2 cut(s)	196, 422
BsuRI	GGCC	5 cut(s)	198, 424, 682, 722, 980
BtgI	CCRYGG	2 cut(s)	717, 864
BtrI	CACGTC	2 cut(s)	445, 742
BtsCI	GGATG	4 cut(s)	438, 1011, 1314, 1380
BtuMI	TCGCGA	2 cut(s)	1055, 1163
BveI	ACCTGC	1 cut(s)	1087
Cac8I	GCNNGC	3 cut(s)	176, 351, 1388
CfoI	GCGC	4 cut(s)	70, 508, 926, 1238
Cfr13I	GGNCC	4 cut(s)	680, 754, 841, 964
Cfr42I	CCGCGG	1 cut(s)	867
CseI	GACGC	1 cut(s)	512
Csp6I	GTAC	6 cut(s)	73, 631, 690, 736, 745, 1360
CspCI	CAANNNNNGTGG	2 cut(s)	45, 80
CviAII	CATG	6 cut(s)	134, 175, 493, 718, 898, 940
CviQI	GTAC	6 cut(s)	73, 631, 690, 736, 745, 1360
DdeI	CTNAG	4 cut(s)	336, 872, 1115, 1404
DpnI	GATC	6 cut(s)	311, 579, 750, 1001, 1145, 1160
DpnII	GATC	6 cut(s)	309, 577, 748, 999, 1143, 1158
EaeI	YGGCCR	3 cut(s)	196, 422, 720
EagI	CGGCCG	2 cut(s)	196, 422
Eam1104I	CTCTTC	1 cut(s)	1094
EarI	CTCTTC	1 cut(s)	1094
EciI	GGCGGA	1 cut(s)	427
Ecl136II	GAGCTC	1 cut(s)	460
EclXI	CGGCCG	2 cut(s)	196, 422
Eco130I	CCWWGG	1 cut(s)	717
Eco24I	GRGCYC	1 cut(s)	462
Eco31I	GGTCTC	1 cut(s)	551
Eco47I	GGWCC	3 cut(s)	754, 841, 964
Eco52I	CGGCCG	2 cut(s)	196, 422
Eco53kI	GAGCTC	1 cut(s)	460
Eco57I	CTGAAG	1 cut(s)	806
Eco88I	CYCGRG	3 cut(s)	461, 731, 1067
EcoICRI	GAGCTC	1 cut(s)	460
EcoRI	GAATTC	2 cut(s)	913, 955
EcoT14I	CCWWGG	1 cut(s)	717
EcoT38I	GRGCYC	1 cut(s)	462
ErhI	CCWWGG	1 cut(s)	717
FaeI	CATG	6 cut(s)	137, 178, 496, 721, 901, 943
FaqI	GGGAC	2 cut(s)	140, 515
FatI	CATG	6 cut(s)	133, 174, 492, 717, 897, 939
FauI	CCCGC	7 cut(s)	756, 859, 871, 1112, 1231, 1248, 1298
FblI	GTMKAC	3 cut(s)	138, 501, 548
FokI	GGATG	4 cut(s)	425, 998, 1321, 1387
FriOI	GRGCYC	1 cut(s)	462
FspBI	CTAG	1 cut(s)	644
GlaI	GCGC	4 cut(s)	69, 507, 925, 1237
GsuI	CTGGAG	2 cut(s)	275, 1196
HaeII	RGCGCY	1 cut(s)	71
HaeIII	GGCC	5 cut(s)	198, 424, 682, 722, 980
HapII	CCGG	7 cut(s)	264, 509, 977, 992, 1136, 1168, 1302
HgaI	GACGC	1 cut(s)	512
HhaI	GCGC	4 cut(s)	70, 508, 926, 1238
Hin1I	GRCGYC	1 cut(s)	1005
Hin1II	CATG	6 cut(s)	137, 178, 496, 721, 901, 943
Hin6I	GCGC	4 cut(s)	68, 506, 924, 1236
HinP1I	GCGC	4 cut(s)	68, 506, 924, 1236
HincII	GTYRAC	3 cut(s)	502, 549, 973
HindII	GTYRAC	3 cut(s)	502, 549, 973
HindIII	AAGCTT	1 cut(s)	485
HpaII	CCGG	7 cut(s)	264, 509, 977, 992, 1136, 1168, 1302
HphI	GGTGA	9 cut(s)	196, 217, 241, 253, 352, 533, 629, 1084, 1307
Hpy166II	GTNNAC	9 cut(s)	91, 139, 502, 549, 745, 948, 973, 1009, 1298
Hpy8I	GTNNAC	9 cut(s)	91, 139, 502, 549, 745, 948, 973, 1009, 1298
HpyAV	CCTTC	9 cut(s)	382, 544, 562, 955, 991, 1069, 1245, 1316, 1430
HpyCH4III	ACNGT	1 cut(s)	630
HpyCH4IV	ACGT	5 cut(s)	444, 498, 741, 969, 1005
HpyCH4V	TGCA	3 cut(s)	178, 433, 802
HpyF10VI	GCNNNNNNNGC	4 cut(s)	430, 679, 719, 1093
HpyF3I	CTNAG	4 cut(s)	336, 872, 1115, 1404
HpySE526I	ACGT	5 cut(s)	444, 498, 741, 969, 1005
Hsp92I	GRCGYC	1 cut(s)	1005
Hsp92II	CATG	6 cut(s)	137, 178, 496, 721, 901, 943
HspAI	GCGC	4 cut(s)	68, 506, 924, 1236
KspI	CCGCGG	1 cut(s)	867
Kzo9I	GATC	6 cut(s)	309, 577, 748, 999, 1143, 1158
LguI	GCTCTTC	1 cut(s)	1094
LmnI	GCTCC	4 cut(s)	663, 781, 1037, 1078
Lsp1109I	GCAGC	4 cut(s)	445, 894, 908, 1395
MaeI	CTAG	1 cut(s)	644
MaeII	ACGT	5 cut(s)	444, 498, 741, 969, 1005
MaeIII	GTNAC	3 cut(s)	34, 202, 259
MalI	GATC	6 cut(s)	311, 579, 750, 1001, 1145, 1160
MbiI	CCGCTC	2 cut(s)	1024, 1119
MboI	GATC	6 cut(s)	309, 577, 748, 999, 1143, 1158
MboII	GAAGA	5 cut(s)	248, 481, 785, 799, 1081
MhlI	GDGCHC	2 cut(s)	462, 1083
MlsI	TGGCCA	1 cut(s)	722
MluCI	AATT	6 cut(s)	282, 322, 724, 892, 913, 955
MluNI	TGGCCA	1 cut(s)	722
MlyI	GAGTC	6 cut(s)	210, 581, 617, 945, 1141, 1189
MmeI	TCCRAC	2 cut(s)	977, 1012
Mox20I	TGGCCA	1 cut(s)	722
MroXI	GAANNNNTTC	1 cut(s)	285
MscI	TGGCCA	1 cut(s)	722
MseI	TTAA	1 cut(s)	1191
MslI	CAYNNNNRTG	1 cut(s)	566
Msp20I	TGGCCA	1 cut(s)	722
MspA1I	CMGCKG	1 cut(s)	866
MspI	CCGG	7 cut(s)	264, 509, 977, 992, 1136, 1168, 1302
MspR9I	CCNGG	3 cut(s)	977, 993, 1302
Mva1269I	GAATGC	1 cut(s)	1040
MvnI	CGCG	7 cut(s)	63, 506, 763, 866, 1055, 1163, 1238
MwoI	GCNNNNNNNGC	4 cut(s)	430, 679, 719, 1093
NciI	CCSGG	3 cut(s)	977, 993, 1302
NcoI	CCATGG	1 cut(s)	717
NdeII	GATC	6 cut(s)	309, 577, 748, 999, 1143, 1158
NlaIII	CATG	6 cut(s)	137, 178, 496, 721, 901, 943
NmeAIII	GCCGAG	4 cut(s)	224, 880, 952, 1203
NmuCI	GTSAC	2 cut(s)	202, 259
NruI	TCGCGA	2 cut(s)	1055, 1163
NspI	RCATGY	1 cut(s)	178
NspV	TTCGAA	1 cut(s)	959
PaeI	GCATGC	1 cut(s)	178
PaeR7I	CTCGAG	1 cut(s)	461
PaqCI	CACCTGC	1 cut(s)	1087
PciSI	GCTCTTC	1 cut(s)	1094
PcsI	WCGNNNNNNNCGW	5 cut(s)	453, 738, 744, 887, 956
PctI	GAATGC	1 cut(s)	1040
PdmI	GAANNNNTTC	1 cut(s)	285
PfeI	GAWTC	7 cut(s)	4, 184, 239, 415, 776, 790, 1289
PflFI	GACNNNGTC	2 cut(s)	645, 993
PleI	GAGTC	6 cut(s)	209, 580, 616, 944, 1140, 1188
PpsI	GAGTC	6 cut(s)	209, 580, 616, 944, 1140, 1188
Psp124BI	GAGCTC	1 cut(s)	462
PspPI	GGNCC	4 cut(s)	680, 754, 841, 964
PspXI	VCTCGAGB	1 cut(s)	461
PsyI	GACNNNGTC	2 cut(s)	645, 993
RruI	TCGCGA	2 cut(s)	1055, 1163
RsaI	GTAC	6 cut(s)	74, 632, 691, 737, 746, 1361
RsaNI	GTAC	6 cut(s)	73, 631, 690, 736, 745, 1360
RseI	CAYNNNNRTG	1 cut(s)	566
SacI	GAGCTC	1 cut(s)	462
SacII	CCGCGG	1 cut(s)	867
SalI	GTCGAC	2 cut(s)	500, 547
SapI	GCTCTTC	1 cut(s)	1094
SaqAI	TTAA	1 cut(s)	1191
Sau3AI	GATC	6 cut(s)	309, 577, 748, 999, 1143, 1158
Sau96I	GGNCC	4 cut(s)	680, 754, 841, 964
ScaI	AGTACT	1 cut(s)	632
SchI	GAGTC	6 cut(s)	210, 581, 617, 945, 1141, 1189
ScrFI	CCNGG	3 cut(s)	977, 993, 1302
SduI	GDGCHC	2 cut(s)	462, 1083
Sfr274I	CTCGAG	1 cut(s)	461
Sfr303I	CCGCGG	1 cut(s)	867
SfuI	TTCGAA	1 cut(s)	959
SgrBI	CCGCGG	1 cut(s)	867
SgrDI	CGTCGACG	1 cut(s)	500
SinI	GGWCC	3 cut(s)	754, 841, 964
SlaI	CTCGAG	1 cut(s)	461
SmiMI	CAYNNNNRTG	1 cut(s)	566
SmlI	CTYRAG	3 cut(s)	461, 617, 1369
SmoI	CTYRAG	3 cut(s)	461, 617, 1369
SphI	GCATGC	1 cut(s)	178
Sse9I	AATT	6 cut(s)	282, 322, 724, 892, 913, 955
SspMI	CTAG	1 cut(s)	644
SstI	GAGCTC	1 cut(s)	462
StyD4I	CCNGG	3 cut(s)	975, 991, 1300
StyI	CCWWGG	1 cut(s)	717
TaaI	ACNGT	1 cut(s)	630
TaiI	ACGT	5 cut(s)	447, 501, 744, 972, 1008
TaqII	GACCGA	3 cut(s)	808, 858, 1186
TasI	AATT	6 cut(s)	282, 322, 724, 892, 913, 955
TatI	WGTACW	2 cut(s)	72, 630
TauI	GCSGC	6 cut(s)	115, 363, 366, 412, 716, 1429
TfiI	GAWTC	7 cut(s)	4, 184, 239, 415, 776, 790, 1289
Tru1I	TTAA	1 cut(s)	1191
Tru9I	TTAA	1 cut(s)	1191
TseFI	GTSAC	2 cut(s)	202, 259
TseI	GCWGC	4 cut(s)	433, 907, 921, 1383
Tsp45I	GTSAC	2 cut(s)	202, 259
TspDTI	ATGAA	4 cut(s)	509, 886, 968, 1391
TspGWI	ACGGA	2 cut(s)	676, 1232
Tth111I	GACNNNGTC	2 cut(s)	645, 993
VpaK11BI	GGWCC	3 cut(s)	754, 841, 964
XapI	RAATTY	3 cut(s)	892, 913, 955
XceI	RCATGY	1 cut(s)	178
XhoI	CTCGAG	1 cut(s)	461
XmiI	GTMKAC	3 cut(s)	138, 501, 548
XmnI	GAANNNNTTC	1 cut(s)	285
XspI	CTAG	1 cut(s)	644
ZraI	GACGTC	1 cut(s)	1006
ZrmI	AGTACT	1 cut(s)	632

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.