Domain associated at C-terminal with AAA
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 1715 |
| AccB1I | GGYRCC | 1 cut(s) | 1715 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 561 |
| AccIII | TCCGGA | 2 cut(s) | 1651, 1779 |
| AciI | CCGC | 5 cut(s) | 386, 435, 1751, 2668, 2725 |
| AclWI | GGATC | 3 cut(s) | 1322, 2291, 2627 |
| AcoI | YGGCCR | 2 cut(s) | 225, 1430 |
| AcsI | RAATTY | 6 cut(s) | 26, 616, 1049, 1822, 1991, 2093 |
| AfaI | GTAC | 9 cut(s) | 42, 274, 342, 390, 991, 1359, 1717, 1801, 2417 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 561, 2376, 2655 |
| AflIII | ACRYGT | 1 cut(s) | 1020 |
| AhlI | ACTAGT | 2 cut(s) | 637, 1928 |
| AjnI | CCWGG | 4 cut(s) | 118, 170, 1300, 2046 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 544, 576 |
| Alw21I | GWGCWC | 2 cut(s) | 102, 664 |
| Alw26I | GTCTC | 7 cut(s) | 151, 758, 837, 839, 1064, 1081, 2146 |
| AlwI | GGATC | 3 cut(s) | 1322, 2291, 2627 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1260, 1414 |
| Ama87I | CYCGRG | 1 cut(s) | 797 |
| Aor13HI | TCCGGA | 2 cut(s) | 1651, 1779 |
| AoxI | GGCC | 5 cut(s) | 225, 1246, 1280, 1430, 1593 |
| ApeKI | GCWGC | 6 cut(s) | 316, 346, 596, 626, 1748, 2434 |
| ApoI | RAATTY | 6 cut(s) | 26, 616, 1049, 1822, 1991, 2093 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 43, 75, 838, 870 |
| AseI | ATTAAT | 1 cut(s) | 165 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 1891, 1991 |
| Asp718I | GGTACC | 1 cut(s) | 1715 |
| AspS9I | GGNCC | 2 cut(s) | 232, 681 |
| AsuC2I | CCSGG | 3 cut(s) | 798, 799, 1350 |
| AsuHPI | GGTGA | 7 cut(s) | 901, 980, 1045, 1133, 1994, 2017, 2036 |
| AsuII | TTCGAA | 3 cut(s) | 857, 1322, 2158 |
| AvaI | CYCGRG | 1 cut(s) | 797 |
| AvaII | GGWCC | 2 cut(s) | 232, 681 |
| AxyI | CCTNAGG | 1 cut(s) | 654 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 264, 264, 297, 297 |
| BalI | TGGCCA | 1 cut(s) | 1432 |
| BanI | GGYRCC | 1 cut(s) | 1715 |
| BanII | GRGCYC | 1 cut(s) | 102 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 333, 365, 2622, 2654 |
| BbsI | GAAGAC | 2 cut(s) | 1673, 1688 |
| Bbv12I | GWGCWC | 2 cut(s) | 102, 664 |
| BbvI | GCAGC | 6 cut(s) | 328, 333, 608, 613, 1735, 2421 |
| BccI | CCATC | 5 cut(s) | 884, 1064, 1112, 1973, 2719 |
| BceAI | ACGGC | 3 cut(s) | 212, 359, 2531 |
| BciT130I | CCWGG | 4 cut(s) | 120, 172, 1302, 2048 |
| BciVI | GTATCC | 2 cut(s) | 292, 1298 |
| BcnI | CCSGG | 3 cut(s) | 798, 799, 1350 |
| BcoDI | GTCTC | 7 cut(s) | 151, 758, 837, 839, 1064, 1081, 2146 |
| BcuI | ACTAGT | 2 cut(s) | 637, 1928 |
| BfaI | CTAG | 5 cut(s) | 36, 638, 1083, 1929, 2438 |
| BfmI | CTRYAG | 3 cut(s) | 91, 588, 2452 |
| BfuI | GTATCC | 2 cut(s) | 292, 1298 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 2730 |
| BisI | GCNGC | 7 cut(s) | 317, 347, 597, 627, 1749, 2435, 2725 |
| BlpI | GCTNAGC | 2 cut(s) | 663, 1961 |
| BlsI | GCNGC | 7 cut(s) | 318, 348, 598, 628, 1750, 2436, 2726 |
| Bme1390I | CCNGG | 7 cut(s) | 120, 172, 798, 799, 1302, 1350, 2048 |
| Bme18I | GGWCC | 2 cut(s) | 232, 681 |
| BmeT110I | CYCGRG | 1 cut(s) | 797 |
| BmgT120I | GGNCC | 2 cut(s) | 232, 681 |
| BmiI | GGNNCC | 3 cut(s) | 682, 1717, 1894 |
| BmrFI | CCNGG | 7 cut(s) | 120, 172, 798, 799, 1302, 1350, 2048 |
| BmsI | GCATC | 6 cut(s) | 446, 1401, 1680, 1962, 2070, 2661 |
| BpiI | GAAGAC | 2 cut(s) | 1673, 1688 |
| BplI | GAGNNNNNCTC | 6 cut(s) | 1061, 1093, 1092, 1124, 1367, 1399 |
| BpmI | CTGGAG | 5 cut(s) | 102, 711, 1019, 1192, 1449 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1012 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 663, 1961 |
| Bpu14I | TTCGAA | 3 cut(s) | 857, 1322, 2158 |
| BpuEI | CTTGAG | 1 cut(s) | 2119 |
| BpuMI | CCSGG | 3 cut(s) | 798, 799, 1350 |
| BsaAI | YACGTR | 1 cut(s) | 878 |
| BsaBI | GATNNNNATC | 1 cut(s) | 894 |
| BsaI | GGTCTC | 2 cut(s) | 758, 837 |
| BsaJI | CCNNGG | 4 cut(s) | 228, 797, 1695, 2527 |
| BsaWI | WCCGGW | 2 cut(s) | 1651, 1779 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 561, 2376, 2655 |
| Bse1I | ACTGG | 5 cut(s) | 380, 630, 970, 1162, 2304 |
| Bse21I | CCTNAGG | 1 cut(s) | 654 |
| Bse3DI | GCAATG | 2 cut(s) | 2110, 2570 |
| Bse8I | GATNNNNATC | 1 cut(s) | 894 |
| BseAI | TCCGGA | 2 cut(s) | 1651, 1779 |
| BseBI | CCWGG | 4 cut(s) | 120, 172, 1302, 2048 |
| BseDI | CCNNGG | 4 cut(s) | 228, 797, 1695, 2527 |
| BseGI | GGATG | 5 cut(s) | 1660, 1769, 2085, 2226, 2676 |
| BseJI | GATNNNNATC | 1 cut(s) | 894 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 561, 2376, 2655 |
| BseMI | GCAATG | 2 cut(s) | 2110, 2570 |
| BseMII | CTCAG | 8 cut(s) | 668, 677, 918, 1022, 1026, 2234, 2261, 2364 |
| BseNI | ACTGG | 5 cut(s) | 380, 630, 970, 1162, 2304 |
| BseRI | GAGGAG | 1 cut(s) | 439 |
| BseXI | GCAGC | 6 cut(s) | 328, 333, 608, 613, 1735, 2421 |
| BsgI | GTGCAG | 1 cut(s) | 1246 |
| BshFI | GGCC | 5 cut(s) | 227, 1248, 1282, 1432, 1595 |
| BshNI | GGYRCC | 1 cut(s) | 1715 |
| BsiHKAI | GWGCWC | 2 cut(s) | 102, 664 |
| BsiHKCI | CYCGRG | 1 cut(s) | 797 |
| BsiSI | CCGG | 4 cut(s) | 798, 1349, 1652, 1780 |
| BslFI | GGGAC | 3 cut(s) | 64, 2082, 2631 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 561, 2376, 2655 |
| BsmAI | GTCTC | 7 cut(s) | 151, 758, 837, 839, 1064, 1081, 2146 |
| BsmFI | GGGAC | 3 cut(s) | 64, 2082, 2631 |
| BsmI | GAATGC | 2 cut(s) | 1298, 1828 |
| BsnI | GGCC | 5 cut(s) | 227, 1248, 1282, 1432, 1595 |
| Bso31I | GGTCTC | 2 cut(s) | 758, 837 |
| BsoBI | CYCGRG | 1 cut(s) | 797 |
| Bsp119I | TTCGAA | 3 cut(s) | 857, 1322, 2158 |
| Bsp1286I | GDGCHC | 2 cut(s) | 102, 664 |
| Bsp13I | TCCGGA | 2 cut(s) | 1651, 1779 |
| Bsp1407I | TGTACA | 1 cut(s) | 2415 |
| Bsp143I | GATC | 6 cut(s) | 508, 1273, 1327, 2223, 2283, 2632 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 663, 1961 |
| BspACI | CCGC | 5 cut(s) | 386, 435, 1751, 2668, 2725 |
| BspANI | GGCC | 5 cut(s) | 227, 1248, 1282, 1432, 1595 |
| BspCNI | CTCAG | 8 cut(s) | 667, 676, 919, 1023, 1025, 2233, 2260, 2365 |
| BspEI | TCCGGA | 2 cut(s) | 1651, 1779 |
| BspLI | GGNNCC | 3 cut(s) | 682, 1717, 1894 |
| BspMAI | CTGCAG | 1 cut(s) | 592 |
| BspPI | GGATC | 3 cut(s) | 1322, 2291, 2627 |
| BspT104I | TTCGAA | 3 cut(s) | 857, 1322, 2158 |
| BspT107I | GGYRCC | 1 cut(s) | 1715 |
| BspTNI | GGTCTC | 2 cut(s) | 758, 837 |
| BsrDI | GCAATG | 2 cut(s) | 2110, 2570 |
| BsrGI | TGTACA | 1 cut(s) | 2415 |
| BsrI | ACTGG | 5 cut(s) | 380, 630, 970, 1162, 2304 |
| BssECI | CCNNGG | 4 cut(s) | 228, 797, 1695, 2527 |
| BssMI | GATC | 6 cut(s) | 508, 1273, 1327, 2223, 2283, 2632 |
| Bst2UI | CCWGG | 4 cut(s) | 120, 172, 1302, 2048 |
| Bst4CI | ACNGT | 9 cut(s) | 340, 506, 1075, 1219, 1256, 2211, 2341, 2528, 2662 |
| Bst6I | CTCTTC | 1 cut(s) | 2058 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 322 |
| BstAUI | TGTACA | 1 cut(s) | 2415 |
| BstBAI | YACGTR | 1 cut(s) | 878 |
| BstBI | TTCGAA | 3 cut(s) | 857, 1322, 2158 |
| BstC8I | GCNNGC | 2 cut(s) | 1580, 2119 |
| BstDSI | CCRYGG | 3 cut(s) | 228, 1695, 2527 |
| BstF5I | GGATG | 5 cut(s) | 1660, 1769, 2085, 2226, 2676 |
| BstKTI | GATC | 6 cut(s) | 511, 1276, 1330, 2226, 2286, 2635 |
| BstMAI | GTCTC | 7 cut(s) | 151, 758, 837, 839, 1064, 1081, 2146 |
| BstMBI | GATC | 6 cut(s) | 508, 1273, 1327, 2223, 2283, 2632 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 322, 352, 392, 596, 880, 1136, 2730 |
| BstNI | CCWGG | 4 cut(s) | 120, 172, 1302, 2048 |
| BstNSI | RCATGY | 1 cut(s) | 1024 |
| BstSCI | CCNGG | 7 cut(s) | 118, 170, 796, 797, 1300, 1348, 2046 |
| BstSFI | CTRYAG | 3 cut(s) | 91, 588, 2452 |
| BstSNI | TACGTA | 1 cut(s) | 878 |
| BstV1I | GCAGC | 6 cut(s) | 328, 333, 608, 613, 1735, 2421 |
| BstV2I | GAAGAC | 2 cut(s) | 1673, 1688 |
| BstX2I | RGATCY | 2 cut(s) | 2283, 2632 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 86, 404, 1711 |
| BstYI | RGATCY | 2 cut(s) | 2283, 2632 |
| Bsu36I | CCTNAGG | 1 cut(s) | 654 |
| BsuI | GTATCC | 2 cut(s) | 292, 1298 |
| BsuRI | GGCC | 5 cut(s) | 227, 1248, 1282, 1432, 1595 |
| BtgI | CCRYGG | 3 cut(s) | 228, 1695, 2527 |
| BtgZI | GCGATG | 1 cut(s) | 2697 |
| BtsCI | GGATG | 5 cut(s) | 1660, 1769, 2085, 2226, 2676 |
| BtsI | GCAGTG | 2 cut(s) | 1213, 1956 |
| BtsIMutI | CAGTG | 8 cut(s) | 423, 511, 637, 1155, 1194, 1213, 1956, 2216 |
| Cac8I | GCNNGC | 2 cut(s) | 1580, 2119 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1260, 1414 |
| Cfr13I | GGNCC | 2 cut(s) | 232, 681 |
| Cfr9I | CCCGGG | 1 cut(s) | 797 |
| CseI | GACGC | 1 cut(s) | 2341 |
| CsiI | ACCWGGT | 1 cut(s) | 2046 |
| Csp6I | GTAC | 9 cut(s) | 41, 273, 341, 389, 990, 1358, 1716, 1800, 2416 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 294, 329 |
| CviAII | CATG | 7 cut(s) | 453, 1021, 1790, 2204, 2576, 2605, 2706 |
| CviQI | GTAC | 9 cut(s) | 41, 273, 341, 389, 990, 1358, 1716, 1800, 2416 |
| DpnI | GATC | 6 cut(s) | 510, 1275, 1329, 2225, 2285, 2634 |
| DpnII | GATC | 6 cut(s) | 508, 1273, 1327, 2223, 2283, 2632 |
| EaeI | YGGCCR | 2 cut(s) | 225, 1430 |
| Eam1104I | CTCTTC | 1 cut(s) | 2058 |
| EarI | CTCTTC | 1 cut(s) | 2058 |
| Ecl136II | GAGCTC | 1 cut(s) | 100 |
| Eco105I | TACGTA | 1 cut(s) | 878 |
| Eco147I | AGGCCT | 1 cut(s) | 1595 |
| Eco24I | GRGCYC | 1 cut(s) | 102 |
| Eco31I | GGTCTC | 2 cut(s) | 758, 837 |
| Eco32I | GATATC | 1 cut(s) | 808 |
| Eco47I | GGWCC | 2 cut(s) | 232, 681 |
| Eco53kI | GAGCTC | 1 cut(s) | 100 |
| Eco81I | CCTNAGG | 1 cut(s) | 654 |
| Eco88I | CYCGRG | 1 cut(s) | 797 |
| EcoICRI | GAGCTC | 1 cut(s) | 100 |
| EcoRI | GAATTC | 2 cut(s) | 1991, 2093 |
| EcoRII | CCWGG | 4 cut(s) | 118, 170, 1300, 2046 |
| EcoRV | GATATC | 1 cut(s) | 808 |
| EcoT38I | GRGCYC | 1 cut(s) | 102 |
| FaeI | CATG | 7 cut(s) | 456, 1024, 1793, 2207, 2579, 2608, 2709 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 594, 626, 1362, 1394 |
| FaqI | GGGAC | 3 cut(s) | 64, 2082, 2631 |
| FatI | CATG | 7 cut(s) | 452, 1020, 1789, 2203, 2575, 2604, 2705 |
| FauNDI | CATATG | 3 cut(s) | 277, 1202, 2173 |
| Fnu4HI | GCNGC | 7 cut(s) | 317, 347, 597, 627, 1749, 2435, 2725 |
| FokI | GGATG | 5 cut(s) | 1667, 1776, 2092, 2213, 2683 |
| FriOI | GRGCYC | 1 cut(s) | 102 |
| Fsp4HI | GCNGC | 7 cut(s) | 317, 347, 597, 627, 1749, 2435, 2725 |
| FspBI | CTAG | 5 cut(s) | 36, 638, 1083, 1929, 2438 |
| GluI | GCNGC | 7 cut(s) | 317, 347, 597, 627, 1749, 2435, 2725 |
| GsuI | CTGGAG | 5 cut(s) | 102, 711, 1019, 1192, 1449 |
| HaeIII | GGCC | 5 cut(s) | 227, 1248, 1282, 1432, 1595 |
| HapII | CCGG | 4 cut(s) | 798, 1349, 1652, 1780 |
| HgaI | GACGC | 1 cut(s) | 2341 |
| Hin1II | CATG | 7 cut(s) | 456, 1024, 1793, 2207, 2579, 2608, 2709 |
| HindIII | AAGCTT | 3 cut(s) | 824, 2119, 2306 |
| HinfI | GANTC | 9 cut(s) | 376, 541, 867, 971, 1319, 1700, 1869, 2035, 2320 |
| HpaII | CCGG | 4 cut(s) | 798, 1349, 1652, 1780 |
| HphI | GGTGA | 7 cut(s) | 901, 980, 1045, 1133, 1994, 2017, 2036 |
| Hpy166II | GTNNAC | 7 cut(s) | 235, 608, 635, 1909, 2008, 2044, 2416 |
| Hpy8I | GTNNAC | 7 cut(s) | 235, 608, 635, 1909, 2008, 2044, 2416 |
| Hpy99I | CGWCG | 1 cut(s) | 1219 |
| HpyAV | CCTTC | 9 cut(s) | 474, 709, 778, 1467, 1606, 1606, 1881, 1981, 2672 |
| HpyCH4III | ACNGT | 9 cut(s) | 340, 506, 1075, 1219, 1256, 2211, 2341, 2528, 2662 |
| HpyCH4IV | ACGT | 2 cut(s) | 877, 2010 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 322, 352, 392, 596, 880, 1136, 2730 |
| HpySE526I | ACGT | 2 cut(s) | 877, 2010 |
| Hsp92II | CATG | 7 cut(s) | 456, 1024, 1793, 2207, 2579, 2608, 2709 |
| Kpn2I | TCCGGA | 2 cut(s) | 1651, 1779 |
| KpnI | GGTACC | 1 cut(s) | 1719 |
| Kzo9I | GATC | 6 cut(s) | 508, 1273, 1327, 2223, 2283, 2632 |
| LmnI | GCTCC | 3 cut(s) | 105, 1086, 1970 |
| Lsp1109I | GCAGC | 6 cut(s) | 328, 333, 608, 613, 1735, 2421 |
| LweI | GCATC | 6 cut(s) | 446, 1401, 1680, 1962, 2070, 2661 |
| MabI | ACCWGGT | 1 cut(s) | 2046 |
| MaeI | CTAG | 5 cut(s) | 36, 638, 1083, 1929, 2438 |
| MaeII | ACGT | 2 cut(s) | 877, 2010 |
| MaeIII | GTNAC | 6 cut(s) | 109, 907, 956, 968, 1548, 1838 |
| MalI | GATC | 6 cut(s) | 510, 1275, 1329, 2225, 2285, 2634 |
| MboI | GATC | 6 cut(s) | 508, 1273, 1327, 2223, 2283, 2632 |
| MflI | RGATCY | 2 cut(s) | 2283, 2632 |
| MhlI | GDGCHC | 2 cut(s) | 102, 664 |
| MlsI | TGGCCA | 1 cut(s) | 1432 |
| MluNI | TGGCCA | 1 cut(s) | 1432 |
| MlyI | GAGTC | 2 cut(s) | 965, 1709 |
| MmeI | TCCRAC | 4 cut(s) | 1517, 2032, 2129, 2520 |
| Mox20I | TGGCCA | 1 cut(s) | 1432 |
| MroI | TCCGGA | 2 cut(s) | 1651, 1779 |
| MroXI | GAANNNNTTC | 2 cut(s) | 1891, 1991 |
| MscI | TGGCCA | 1 cut(s) | 1432 |
| MseI | TTAA | 8 cut(s) | 165, 245, 1380, 1584, 1731, 1742, 2312, 2501 |
| Msp20I | TGGCCA | 1 cut(s) | 1432 |
| MspI | CCGG | 4 cut(s) | 798, 1349, 1652, 1780 |
| MspR9I | CCNGG | 7 cut(s) | 120, 172, 798, 799, 1302, 1350, 2048 |
| Mva1269I | GAATGC | 2 cut(s) | 1298, 1828 |
| MvaI | CCWGG | 4 cut(s) | 120, 172, 1302, 2048 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 322, 352, 392, 596, 880, 1136, 2730 |
| NciI | CCSGG | 3 cut(s) | 798, 799, 1350 |
| NdeI | CATATG | 3 cut(s) | 277, 1202, 2173 |
| NdeII | GATC | 6 cut(s) | 508, 1273, 1327, 2223, 2283, 2632 |
| NlaIII | CATG | 7 cut(s) | 456, 1024, 1793, 2207, 2579, 2608, 2709 |
| NlaIV | GGNNCC | 3 cut(s) | 682, 1717, 1894 |
| NmuCI | GTSAC | 4 cut(s) | 109, 907, 956, 968 |
| NspI | RCATGY | 1 cut(s) | 1024 |
| NspV | TTCGAA | 3 cut(s) | 857, 1322, 2158 |
| PceI | AGGCCT | 1 cut(s) | 1595 |
| PciI | ACATGT | 1 cut(s) | 1020 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1328 |
| PctI | GAATGC | 2 cut(s) | 1298, 1828 |
| PdmI | GAANNNNTTC | 2 cut(s) | 1891, 1991 |
| PfeI | GAWTC | 7 cut(s) | 376, 541, 867, 1319, 1869, 2035, 2320 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 561 |
| PkrI | GCNGC | 7 cut(s) | 318, 348, 598, 628, 1750, 2436, 2726 |
| PleI | GAGTC | 2 cut(s) | 965, 1708 |
| PpsI | GAGTC | 2 cut(s) | 965, 1708 |
| Ppu21I | YACGTR | 1 cut(s) | 878 |
| PscI | ACATGT | 1 cut(s) | 1020 |
| PshBI | ATTAAT | 1 cut(s) | 165 |
| Psp124BI | GAGCTC | 1 cut(s) | 102 |
| Psp6I | CCWGG | 4 cut(s) | 118, 170, 1300, 2046 |
| PspGI | CCWGG | 4 cut(s) | 118, 170, 1300, 2046 |
| PspN4I | GGNNCC | 3 cut(s) | 682, 1717, 1894 |
| PspPI | GGNCC | 2 cut(s) | 232, 681 |
| PstI | CTGCAG | 1 cut(s) | 592 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1260, 1414 |
| PsuI | RGATCY | 2 cut(s) | 2283, 2632 |
| RsaI | GTAC | 9 cut(s) | 42, 274, 342, 390, 991, 1359, 1717, 1801, 2417 |
| RsaNI | GTAC | 9 cut(s) | 41, 273, 341, 389, 990, 1358, 1716, 1800, 2416 |
| SacI | GAGCTC | 1 cut(s) | 102 |
| SaqAI | TTAA | 8 cut(s) | 165, 245, 1380, 1584, 1731, 1742, 2312, 2501 |
| SatI | GCNGC | 7 cut(s) | 317, 347, 597, 627, 1749, 2435, 2725 |
| Sau3AI | GATC | 6 cut(s) | 508, 1273, 1327, 2223, 2283, 2632 |
| Sau96I | GGNCC | 2 cut(s) | 232, 681 |
| SchI | GAGTC | 2 cut(s) | 965, 1709 |
| ScrFI | CCNGG | 7 cut(s) | 120, 172, 798, 799, 1302, 1350, 2048 |
| SduI | GDGCHC | 2 cut(s) | 102, 664 |
| SexAI | ACCWGGT | 1 cut(s) | 2046 |
| SfaNI | GCATC | 6 cut(s) | 446, 1401, 1680, 1962, 2070, 2661 |
| SfcI | CTRYAG | 3 cut(s) | 91, 588, 2452 |
| SfuI | TTCGAA | 3 cut(s) | 857, 1322, 2158 |
| SinI | GGWCC | 2 cut(s) | 232, 681 |
| SmaI | CCCGGG | 1 cut(s) | 799 |
| SmlI | CTYRAG | 1 cut(s) | 2098 |
| SmoI | CTYRAG | 1 cut(s) | 2098 |
| SnaBI | TACGTA | 1 cut(s) | 878 |
| SpeI | ACTAGT | 2 cut(s) | 637, 1928 |
| SseBI | AGGCCT | 1 cut(s) | 1595 |
| SsiI | CCGC | 5 cut(s) | 386, 435, 1751, 2668, 2725 |
| SspI | AATATT | 1 cut(s) | 1226 |
| SspMI | CTAG | 5 cut(s) | 36, 638, 1083, 1929, 2438 |
| SstI | GAGCTC | 1 cut(s) | 102 |
| StuI | AGGCCT | 1 cut(s) | 1595 |
| StyD4I | CCNGG | 7 cut(s) | 118, 170, 796, 797, 1300, 1348, 2046 |
| TaaI | ACNGT | 9 cut(s) | 340, 506, 1075, 1219, 1256, 2211, 2341, 2528, 2662 |
| TaiI | ACGT | 2 cut(s) | 880, 2013 |
| TaqI | TCGA | 9 cut(s) | 771, 857, 870, 1322, 1679, 1872, 2158, 2262, 2676 |
| TatI | WGTACW | 1 cut(s) | 2415 |
| TauI | GCSGC | 1 cut(s) | 2727 |
| TfiI | GAWTC | 7 cut(s) | 376, 541, 867, 1319, 1869, 2035, 2320 |
| Tru1I | TTAA | 8 cut(s) | 165, 245, 1380, 1584, 1731, 1742, 2312, 2501 |
| Tru9I | TTAA | 8 cut(s) | 165, 245, 1380, 1584, 1731, 1742, 2312, 2501 |
| TscAI | CASTG | 8 cut(s) | 423, 511, 637, 1162, 1194, 1213, 1956, 2216 |
| TseFI | GTSAC | 4 cut(s) | 109, 907, 956, 968 |
| TseI | GCWGC | 6 cut(s) | 316, 346, 596, 626, 1748, 2434 |
| Tsp45I | GTSAC | 4 cut(s) | 109, 907, 956, 968 |
| TspDTI | ATGAA | 8 cut(s) | 231, 1416, 1588, 1762, 1821, 2106, 2220, 2333 |
| TspGWI | ACGGA | 2 cut(s) | 1439, 2092 |
| TspMI | CCCGGG | 1 cut(s) | 797 |
| TspRI | CASTG | 8 cut(s) | 423, 511, 637, 1162, 1194, 1213, 1956, 2216 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 561 |
| VpaK11BI | GGWCC | 2 cut(s) | 232, 681 |
| VspI | ATTAAT | 1 cut(s) | 165 |
| XapI | RAATTY | 6 cut(s) | 26, 616, 1049, 1822, 1991, 2093 |
| XceI | RCATGY | 1 cut(s) | 1024 |
| XmaI | CCCGGG | 1 cut(s) | 797 |
| XmnI | GAANNNNTTC | 2 cut(s) | 1891, 1991 |
| XspI | CTAG | 5 cut(s) | 36, 638, 1083, 1929, 2438 |