Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1B

Orthogroup ID

OG0001360

Physical Location & Seq

Genomic Coordinates Reverse (-)

38749690 .. 38752717

Gene Structure 3028 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1BG257100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1482 bp

ATGATTCTTTCCAACCCATTCTTTCTGCTAGTGTTGGCGGTCTGTATATTCGTGGCGGTTCGTGTGCTTCTGTACAAAACTGGGTTGGTTTATATTGTGAAGAAATGGTGGCGGGCGGTGGAGGACTGCTTCCATGTCTACCAGTCGTTCAAAGTCCCGGAATTCAACGAAAGTATGCAGGAGAATCAGCTCTACTGCAAGGTCACTGTTTATCTCAACTCATTGACCTCCATTGAGGACTCCGATTTCACCAATCTCGTCACCGGAAAAAAGCCCAACGAGATCGTGCTCCAGCTCGACCCCAACCAGACCATCGAAGACGACTTTCTCGGGGCCAAGGTCACGTGGCAAAGCGAAGCGAGTAAGGGTTGTTCCAATAATTGCCGGAATTTGGTGTTGAAGGTTCGGAAAGCCGATAAGCGGCGGATTCTGCGGCCGTATTTGCAGCACATCCACGTCGTCGCCGACGAGCTGGACCAGCGGAAGCGAGATTTGCGGCTGTACATGAACGTCGACGACTCCGCCACGGCCGGCGACGACTTCCCCAACGCGCGGTGGAGGGCCGTGCCTTTCACGCACCCATCTACGCTGGAGACGATTGCCATGGAAGCCGACCTCAAGTCGAAGATCAAGTCCGATTTGGAGTCGTTTCTCAAAGCCAAGCAGTACTACAGCCGACTCGGCCGCGTCTGGAAGCGAAGCTTCTTGCTTTACGGCCCCTCCGGCACCGGGAAATCCAGCTTCGTCGCCGCCATGGCCAATTTCCTCGGCTACGACGTCTACGATCTCGACCTCTCTAGGGTTAAAGACGACTCGGAGTTGAAGATGCTTCTTTTACAGACCACGAGCAAGTCGGTGATCCTCATCGAGGACCTGGATCGGTTCTTCGCCGAGAAATCGACGGCGGTCAGCTTCTCCGGCATATCGAACTTCATGGACGGCTTGCTCAACTCGTGCTGCGCCGAAGAGAGAGTCATGGTGTTCACGATGAACTCGAAGGACCAGGTCGACCCGAATTTCCTCCGACCGGGTCGGATCGACGTCCACATCCATTTTCCGTTATGTGATTTCACGGCGTTTCGGAACCTAGCGACGAGCTATTTGGGGGTGAAGGAGCACAAGCTGTTCCCCCAGGTGGAGGAGATTTTCCAATCCGGGTCGAGTTTGAGCCCGGCCGAGATCGGCGAGCTCATGATCGCGAACCGGAACTCGCCGAGCCGGGCGATCAAGTCGGTCATCACGGCGTTGCAAACCGACGGTGATGGGAGGGGGGCGGGGAAGATCGGACGGCCGCCGAGGTGGTTCGAAAGCGGGTCGAGAAAGTCCGGGGGGGACGAGTCGGGCGAACCCGGAGTTGTGTTATGTGGGGAAGGTGTGAATACGGTTAGTAGGGATTTTAGAAAGCTTTACGGGTTTTTGAGGATGAGAGGCAGCAAGAATTCTCAGTCGTTCGATGCGGGGTCGGAGCGGTCAGCGGGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0006996 GO:0007005 GO:0008150 GO:0009987 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017004 GO:0017062 GO:0017111 GO:0019866 GO:0022607 GO:0031090 GO:0031966 GO:0031967 GO:0031975 GO:0033108 GO:0034551 GO:0034622 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0065003 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA AAA_assoc

Protein Analysis

493

Amino Acids

55.49

Weight (kDa)

8.42

Isoelectric Point (pI)

44.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA_assoc	PF14363	31 - 118	1.2e-13	Domain associated at C-terminal with AAA
AAA	PF00004	236 - 354	3.7e-16	ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540	PF25568	355 - 418	3.2e-11	At3g28540-like, AAA+ ATPase lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001360)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46620
fragaria_vesca	FvH4_7g17220 FvH4_7g32500
malus_domestica	MD01G1085900.v1.1 MD01G1225700.v1.1 MD02G1229200.v1.1 MD07G1155000.v1.1 MD07G1296200.v1.1
prunus_persica	Prupe.2G193700_v2.0.a1 Prupe.2G315800_v2.0.a1
pyrus_communis	pycom01g23420 pycom07g15210 pycom07g26900
rosa_chinensis	RchiOBHm_Chr1g0360611 RchiOBHm_Chr1g0382341 RchiOBHm_Chr2g0086831 RchiOBHm_Chr2g0139501 RchiOBHm_Chr3g0458211 RchiOBHm_Chr3g0478421 RchiOBHm_Chr4g0427361
rosa_laevigata	RLG00000002572 RLG00000009827 RLG00000026185 RLG00000027795 RLG00000030781
rosa_multiflora	Rmu_sc0000110.1_g000040 Rmu_sc0001436.1_g000033 Rmu_sc0002188.1_g000003 Rmu_sc0002535.1_g000007 Rmu_sc0003020.1_g000016 Rmu_sc0003481.1_g000013 Rmu_sc0004697.1_g000002 Rmu_sc0007558.1_g000001 Rmu_sc0010192.1_g000001 Rmu_sc0015587.1_g000001 Rmu_sc0042341.1_g000002
rosa_roxburghii	Rroxscaffold_2G00093530 Rroxscaffold_4G00278180
rosa_rugosa	Rorug01G0280000 Rorug01G0433500 Rorug02G0149500 Rorug05G0571400
rosa_samantha	Rh1AG292200 Rh1BG257100 Rh1BG414500 Rh1CG274900 Rh1DG287000 Rh1DG446600 Rh5BG487600 Rh5CG511800
rosa_wichuraiana	Rw1G025870 Rw1G041280 Rw5G043520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	780, 1044
AccB1I	GGYRCC	1 cut(s)	725
AccBSI	CCGCTC	1 cut(s)	1468
AccI	GTMKAC	4 cut(s)	138, 513, 780, 1008
AccII	CGCG	4 cut(s)	551, 553, 687, 1199
AclWI	GGATC	3 cut(s)	853, 885, 1043
AcoI	YGGCCR	6 cut(s)	434, 528, 682, 756, 1173, 1289
AcsI	RAATTY	4 cut(s)	161, 388, 1015, 1438
AcvI	CACGTG	1 cut(s)	345
AcyI	GRCGYC	2 cut(s)	777, 1041
AfaI	GTAC	3 cut(s)	74, 503, 668
AfiI	CCNNNNNNNGG	9 cut(s)	391, 420, 552, 729, 799, 868, 1027, 1135, 1138
AgsI	TTSAA	4 cut(s)	151, 166, 400, 823
AhdI	GACNNNNNGTC	1 cut(s)	619
AjiI	CACGTC	1 cut(s)	457
AjnI	CCWGG	3 cut(s)	873, 1002, 1131
Alw21I	GWGCWC	3 cut(s)	291, 1119, 1191
Alw26I	GTCTC	1 cut(s)	587
AlwI	GGATC	3 cut(s)	853, 885, 1043
Ama87I	CYCGRG	1 cut(s)	329
AoxI	GGCC	9 cut(s)	333, 434, 528, 561, 682, 715, 756, 1173, 1289
ApeKI	GCWGC	3 cut(s)	445, 957, 1431
ApoI	RAATTY	4 cut(s)	161, 388, 1015, 1438
ArsI	GACNNNNNNTTYG	6 cut(s)	617, 649, 957, 989, 1084, 1116
AspLEI	GCGC	2 cut(s)	553, 962
AspS9I	GGNCC	6 cut(s)	333, 475, 561, 716, 871, 1000
AsuC2I	CCSGG	8 cut(s)	158, 730, 1029, 1156, 1172, 1220, 1327, 1350
AsuHPI	GGTGA	5 cut(s)	241, 253, 868, 1120, 1271
AsuII	TTCGAA	1 cut(s)	1305
AvaI	CYCGRG	1 cut(s)	329
AvaII	GGWCC	3 cut(s)	475, 871, 1000
BalI	TGGCCA	1 cut(s)	758
BanI	GGYRCC	1 cut(s)	725
BanII	GRGCYC	2 cut(s)	1172, 1191
BauI	CACGAG	2 cut(s)	844, 952
BbrPI	CACGTG	1 cut(s)	345
BbsI	GAAGAC	1 cut(s)	324
Bbv12I	GWGCWC	3 cut(s)	291, 1119, 1191
BbvI	GCAGC	3 cut(s)	457, 944, 1443
BccI	CCATC	3 cut(s)	320, 589, 1256
BceAI	ACGGC	9 cut(s)	421, 543, 548, 730, 918, 955, 1089, 1257, 1304
BciT130I	CCWGG	3 cut(s)	875, 1004, 1133
BcnI	CCSGG	8 cut(s)	158, 730, 1029, 1156, 1172, 1220, 1327, 1350
BcoDI	GTCTC	1 cut(s)	587
BfaI	CTAG	3 cut(s)	29, 798, 1088
BfmI	CTRYAG	1 cut(s)	670
BglI	GCCNNNNNGGC	3 cut(s)	681, 723, 755
BisI	GCNGC	9 cut(s)	422, 434, 446, 497, 685, 750, 958, 1292, 1432
BlsI	GCNGC	9 cut(s)	423, 435, 447, 498, 686, 751, 959, 1293, 1433
BmcAI	AGTACT	1 cut(s)	668
Bme18I	GGWCC	3 cut(s)	475, 871, 1000
BmeRI	GACNNNNNGTC	1 cut(s)	619
BmeT110I	CYCGRG	1 cut(s)	329
BmgBI	CACGTC	1 cut(s)	457
BmgT120I	GGNCC	6 cut(s)	333, 475, 561, 716, 871, 1000
BmiI	GGNNCC	4 cut(s)	334, 718, 727, 1085
BmrI	ACTGGG	1 cut(s)	90
BmsI	GCATC	2 cut(s)	816, 1444
BmuI	ACTGGG	1 cut(s)	90
BoxI	GACNNNNGTC	1 cut(s)	905
BpiI	GAAGAC	1 cut(s)	324
BpmI	CTGGAG	2 cut(s)	275, 611
Bpu14I	TTCGAA	1 cut(s)	1305
BpuEI	CTTGAG	1 cut(s)	602
BpuMI	CCSGG	8 cut(s)	158, 730, 1029, 1156, 1172, 1220, 1327, 1350
BsaAI	YACGTR	1 cut(s)	345
BsaBI	GATNNNNATC	1 cut(s)	863
BsaHI	GRCGYC	2 cut(s)	777, 1041
BsaJI	CCNNGG	8 cut(s)	336, 525, 603, 753, 766, 1131, 1295, 1326
BsaWI	WCCGGW	2 cut(s)	263, 1203
BsaXI	ACNNNNNCTCC	2 cut(s)	704, 734
Bsc4I	CCNNNNNNNGG	9 cut(s)	391, 420, 552, 729, 799, 868, 1027, 1135, 1138
Bse118I	RCCGGY	1 cut(s)	530
Bse1I	ACTGG	2 cut(s)	85, 142
Bse8I	GATNNNNATC	1 cut(s)	863
BseBI	CCWGG	3 cut(s)	875, 1004, 1133
BseDI	CCNNGG	8 cut(s)	336, 525, 603, 753, 766, 1131, 1295, 1326
BseGI	GGATG	3 cut(s)	450, 1047, 1428
BseJI	GATNNNNATC	1 cut(s)	863
BseLI	CCNNNNNNNGG	9 cut(s)	391, 420, 552, 729, 799, 868, 1027, 1135, 1138
BseMII	CTCAG	1 cut(s)	1457
BseNI	ACTGG	2 cut(s)	85, 142
BseRI	GAGGAG	1 cut(s)	1154
BseX3I	CGGCCG	5 cut(s)	434, 528, 682, 1173, 1289
BseXI	GCAGC	3 cut(s)	457, 944, 1443
Bsh1236I	CGCG	4 cut(s)	551, 553, 687, 1199
Bsh1285I	CGRYCG	6 cut(s)	437, 531, 685, 1028, 1176, 1292
BshFI	GGCC	9 cut(s)	335, 436, 530, 563, 684, 717, 758, 1175, 1291
BshNI	GGYRCC	1 cut(s)	725
BsiEI	CGRYCG	6 cut(s)	437, 531, 685, 1028, 1176, 1292
BsiHKAI	GWGCWC	3 cut(s)	291, 1119, 1191
BsiHKCI	CYCGRG	1 cut(s)	329
BslFI	GGGAC	2 cut(s)	140, 1346
BslI	CCNNNNNNNGG	9 cut(s)	391, 420, 552, 729, 799, 868, 1027, 1135, 1138
BsmAI	GTCTC	1 cut(s)	587
BsmBI	CGTCTC	1 cut(s)	587
BsmFI	GGGAC	2 cut(s)	140, 1346
BsnI	GGCC	9 cut(s)	335, 436, 530, 563, 684, 717, 758, 1175, 1291
BsoBI	CYCGRG	1 cut(s)	329
Bsp119I	TTCGAA	1 cut(s)	1305
Bsp1286I	GDGCHC	4 cut(s)	291, 1119, 1172, 1191
Bsp1407I	TGTACA	2 cut(s)	72, 501
Bsp19I	CCATGG	2 cut(s)	603, 753
Bsp68I	TCGCGA	1 cut(s)	1199
BspANI	GGCC	9 cut(s)	335, 436, 530, 563, 684, 717, 758, 1175, 1291
BspCNI	CTCAG	1 cut(s)	1456
BspFNI	CGCG	4 cut(s)	551, 553, 687, 1199
BspHI	TCATGA	1 cut(s)	1191
BspLI	GGNNCC	4 cut(s)	334, 718, 727, 1085
BspPI	GGATC	3 cut(s)	853, 885, 1043
BspT104I	TTCGAA	1 cut(s)	1305
BspT107I	GGYRCC	1 cut(s)	725
BsrBI	CCGCTC	1 cut(s)	1468
BsrFI	RCCGGY	1 cut(s)	530
BsrGI	TGTACA	2 cut(s)	72, 501
BsrI	ACTGG	2 cut(s)	85, 142
BssAI	RCCGGY	1 cut(s)	530
BssECI	CCNNGG	8 cut(s)	336, 525, 603, 753, 766, 1131, 1295, 1326
BssNI	GRCGYC	2 cut(s)	777, 1041
BssSI	CACGAG	2 cut(s)	844, 952
BssT1I	CCWWGG	3 cut(s)	336, 603, 753
Bst2BI	CACGAG	2 cut(s)	844, 952
Bst2UI	CCWGG	3 cut(s)	875, 1004, 1133
Bst4CI	ACNGT	3 cut(s)	208, 1259, 1384
Bst6I	CTCTTC	1 cut(s)	960
BstACI	GRCGYC	2 cut(s)	777, 1041
BstAUI	TGTACA	2 cut(s)	72, 501
BstBAI	YACGTR	1 cut(s)	345
BstBI	TTCGAA	1 cut(s)	1305
BstC8I	GCNNGC	5 cut(s)	114, 532, 944, 1187, 1477
BstDEI	CTNAG	1 cut(s)	1443
BstDSI	CCRYGG	3 cut(s)	525, 603, 753
BstENI	CCTNNNNNAGG	1 cut(s)	866
BstF5I	GGATG	3 cut(s)	450, 1047, 1428
BstFNI	CGCG	4 cut(s)	551, 553, 687, 1199
BstHHI	GCGC	2 cut(s)	553, 962
BstMAI	GTCTC	1 cut(s)	587
BstMCI	CGRYCG	6 cut(s)	437, 531, 685, 1028, 1176, 1292
BstNI	CCWGG	3 cut(s)	875, 1004, 1133
BstPAI	GACNNNNGTC	1 cut(s)	905
BstSFI	CTRYAG	1 cut(s)	670
BstUI	CGCG	4 cut(s)	551, 553, 687, 1199
BstV1I	GCAGC	3 cut(s)	457, 944, 1443
BstV2I	GAAGAC	1 cut(s)	324
BstZI	CGGCCG	5 cut(s)	434, 528, 682, 1173, 1289
BsuRI	GGCC	9 cut(s)	335, 436, 530, 563, 684, 717, 758, 1175, 1291
BtgI	CCRYGG	3 cut(s)	525, 603, 753
BtrI	CACGTC	1 cut(s)	457
BtsCI	GGATG	3 cut(s)	450, 1047, 1428
BtsIMutI	CAGTG	1 cut(s)	204
BtuMI	TCGCGA	1 cut(s)	1199
Cac8I	GCNNGC	5 cut(s)	114, 532, 944, 1187, 1477
CciI	TCATGA	1 cut(s)	1191
CfoI	GCGC	2 cut(s)	553, 962
Cfr10I	RCCGGY	1 cut(s)	530
Cfr13I	GGNCC	6 cut(s)	333, 475, 561, 716, 871, 1000
CseI	GACGC	1 cut(s)	676
CsiI	ACCWGGT	1 cut(s)	1002
Csp6I	GTAC	3 cut(s)	73, 502, 667
CviAII	CATG	7 cut(s)	134, 505, 604, 754, 934, 976, 1192
CviQI	GTAC	3 cut(s)	73, 502, 667
DdeI	CTNAG	1 cut(s)	1443
DriI	GACNNNNNGTC	1 cut(s)	619
EaeI	YGGCCR	6 cut(s)	434, 528, 682, 756, 1173, 1289
EagI	CGGCCG	5 cut(s)	434, 528, 682, 1173, 1289
Eam1104I	CTCTTC	1 cut(s)	960
Eam1105I	GACNNNNNGTC	1 cut(s)	619
EarI	CTCTTC	1 cut(s)	960
EciI	GGCGGA	2 cut(s)	439, 511
Ecl136II	GAGCTC	1 cut(s)	1189
EclXI	CGGCCG	5 cut(s)	434, 528, 682, 1173, 1289
Eco130I	CCWWGG	3 cut(s)	336, 603, 753
Eco24I	GRGCYC	2 cut(s)	1172, 1191
Eco47I	GGWCC	3 cut(s)	475, 871, 1000
Eco52I	CGGCCG	5 cut(s)	434, 528, 682, 1173, 1289
Eco53kI	GAGCTC	1 cut(s)	1189
Eco72I	CACGTG	1 cut(s)	345
Eco88I	CYCGRG	1 cut(s)	329
EcoICRI	GAGCTC	1 cut(s)	1189
EcoNI	CCTNNNNNAGG	1 cut(s)	866
EcoO109I	RGGNCCY	1 cut(s)	871
EcoRI	GAATTC	2 cut(s)	161, 1438
EcoRII	CCWGG	3 cut(s)	873, 1002, 1131
EcoT14I	CCWWGG	3 cut(s)	336, 603, 753
EcoT38I	GRGCYC	2 cut(s)	1172, 1191
ErhI	CCWWGG	3 cut(s)	336, 603, 753
Esp3I	CGTCTC	1 cut(s)	587
FaeI	CATG	7 cut(s)	137, 508, 607, 757, 937, 979, 1195
FalI	AAGNNNNNCTT	2 cut(s)	686, 718
FaqI	GGGAC	2 cut(s)	140, 1346
FatI	CATG	7 cut(s)	133, 504, 603, 753, 933, 975, 1191
FauI	CCCGC	5 cut(s)	105, 1267, 1304, 1450, 1468
FblI	GTMKAC	4 cut(s)	138, 513, 780, 1008
Fnu4HI	GCNGC	9 cut(s)	422, 434, 446, 497, 685, 750, 958, 1292, 1432
FokI	GGATG	3 cut(s)	437, 1034, 1435
FriOI	GRGCYC	2 cut(s)	1172, 1191
Fsp4HI	GCNGC	9 cut(s)	422, 434, 446, 497, 685, 750, 958, 1292, 1432
FspBI	CTAG	3 cut(s)	29, 798, 1088
GlaI	GCGC	2 cut(s)	552, 961
GluI	GCNGC	9 cut(s)	422, 434, 446, 497, 685, 750, 958, 1292, 1432
GsuI	CTGGAG	2 cut(s)	275, 611
HaeIII	GGCC	9 cut(s)	335, 436, 530, 563, 684, 717, 758, 1175, 1291
HgaI	GACGC	1 cut(s)	676
HhaI	GCGC	2 cut(s)	553, 962
Hin1I	GRCGYC	2 cut(s)	777, 1041
Hin1II	CATG	7 cut(s)	137, 508, 607, 757, 937, 979, 1195
Hin6I	GCGC	2 cut(s)	551, 960
HinP1I	GCGC	2 cut(s)	551, 960
HincII	GTYRAC	2 cut(s)	514, 1009
HindII	GTYRAC	2 cut(s)	514, 1009
HindIII	AAGCTT	2 cut(s)	700, 1403
HphI	GGTGA	5 cut(s)	241, 253, 868, 1120, 1271
Hpy166II	GTNNAC	6 cut(s)	139, 514, 781, 984, 1009, 1045
Hpy188I	TCNGA	9 cut(s)	244, 408, 637, 817, 1025, 1035, 1083, 1286, 1465
Hpy188III	TCNNGA	6 cut(s)	691, 788, 985, 1192, 1198, 1317
Hpy8I	GTNNAC	6 cut(s)	139, 514, 781, 984, 1009, 1045
HpyAV	CCTTC	4 cut(s)	394, 991, 1105, 1364
HpyCH4III	ACNGT	3 cut(s)	208, 1259, 1384
HpyCH4IV	ACGT	5 cut(s)	344, 456, 510, 777, 1041
HpyCH4V	TGCA	4 cut(s)	178, 198, 445, 1249
HpyF3I	CTNAG	1 cut(s)	1443
HpySE526I	ACGT	5 cut(s)	344, 456, 510, 777, 1041
Hsp92I	GRCGYC	2 cut(s)	777, 1041
Hsp92II	CATG	7 cut(s)	137, 508, 607, 757, 937, 979, 1195
HspAI	GCGC	2 cut(s)	551, 960
KroI	GCCGGC	1 cut(s)	530
KroNI	GCCGGC	1 cut(s)	532
LmnI	GCTCC	3 cut(s)	294, 1114, 1465
Lsp1109I	GCAGC	3 cut(s)	457, 944, 1443
LweI	GCATC	2 cut(s)	816, 1444
MabI	ACCWGGT	1 cut(s)	1002
MaeI	CTAG	3 cut(s)	29, 798, 1088
MaeII	ACGT	5 cut(s)	344, 456, 510, 777, 1041
MaeIII	GTNAC	3 cut(s)	202, 259, 340
MbiI	CCGCTC	1 cut(s)	1468
MboII	GAAGA	7 cut(s)	112, 329, 637, 835, 877, 977, 1291
MhlI	GDGCHC	4 cut(s)	291, 1119, 1172, 1191
MlsI	TGGCCA	1 cut(s)	758
MluCI	AATT	6 cut(s)	161, 379, 388, 760, 1015, 1438
MluNI	TGGCCA	1 cut(s)	758
MlyI	GAGTC	7 cut(s)	233, 512, 653, 672, 806, 981, 1346
MmeI	TCCRAC	4 cut(s)	36, 1013, 1048, 1443
Mox20I	TGGCCA	1 cut(s)	758
MroNI	GCCGGC	1 cut(s)	530
MscI	TGGCCA	1 cut(s)	758
MseI	TTAA	1 cut(s)	804
Msp20I	TGGCCA	1 cut(s)	758
MspA1I	CMGCKG	2 cut(s)	481, 1475
MvaI	CCWGG	3 cut(s)	875, 1004, 1133
MvnI	CGCG	4 cut(s)	551, 553, 687, 1199
NaeI	GCCGGC	1 cut(s)	532
NciI	CCSGG	8 cut(s)	158, 730, 1029, 1156, 1172, 1220, 1327, 1350
NcoI	CCATGG	2 cut(s)	603, 753
NgoMIV	GCCGGC	1 cut(s)	530
NlaIII	CATG	7 cut(s)	137, 508, 607, 757, 937, 979, 1195
NlaIV	GGNNCC	4 cut(s)	334, 718, 727, 1085
NmeAIII	GCCGAG	6 cut(s)	660, 747, 916, 1201, 1239, 1320
NmuCI	GTSAC	3 cut(s)	202, 259, 340
NruI	TCGCGA	1 cut(s)	1199
NspV	TTCGAA	1 cut(s)	1305
PagI	TCATGA	1 cut(s)	1191
PcsI	WCGNNNNNNNCGW	7 cut(s)	462, 465, 593, 774, 780, 992, 1341
PdiI	GCCGGC	1 cut(s)	532
PfeI	GAWTC	3 cut(s)	4, 184, 427
PflFI	GACNNNGTC	2 cut(s)	1004, 1029
PfoI	TCCNGGA	1 cut(s)	156
PkrI	GCNGC	9 cut(s)	423, 435, 447, 498, 686, 751, 959, 1293, 1433
PleI	GAGTC	7 cut(s)	233, 512, 652, 672, 806, 980, 1345
PmaCI	CACGTG	1 cut(s)	345
PmlI	CACGTG	1 cut(s)	345
PpsI	GAGTC	7 cut(s)	233, 512, 652, 672, 806, 980, 1345
Ppu21I	YACGTR	1 cut(s)	345
PpuMI	RGGWCCY	1 cut(s)	871
PshAI	GACNNNNGTC	1 cut(s)	905
Psp124BI	GAGCTC	1 cut(s)	1191
Psp5II	RGGWCCY	1 cut(s)	871
Psp6I	CCWGG	3 cut(s)	873, 1002, 1131
PspCI	CACGTG	1 cut(s)	345
PspGI	CCWGG	3 cut(s)	873, 1002, 1131
PspN4I	GGNNCC	4 cut(s)	334, 718, 727, 1085
PspPI	GGNCC	6 cut(s)	333, 475, 561, 716, 871, 1000
PspPPI	RGGWCCY	1 cut(s)	871
PsrI	GAACNNNNNNTAC	2 cut(s)	355, 387
PsyI	GACNNNGTC	2 cut(s)	1004, 1029
RruI	TCGCGA	1 cut(s)	1199
RsaI	GTAC	3 cut(s)	74, 503, 668
RsaNI	GTAC	3 cut(s)	73, 502, 667
SacI	GAGCTC	1 cut(s)	1191
SalI	GTCGAC	2 cut(s)	512, 1007
SaqAI	TTAA	1 cut(s)	804
SatI	GCNGC	9 cut(s)	422, 434, 446, 497, 685, 750, 958, 1292, 1432
Sau96I	GGNCC	6 cut(s)	333, 475, 561, 716, 871, 1000
ScaI	AGTACT	1 cut(s)	668
SchI	GAGTC	7 cut(s)	233, 512, 653, 672, 806, 981, 1346
SduI	GDGCHC	4 cut(s)	291, 1119, 1172, 1191
SexAI	ACCWGGT	1 cut(s)	1002
SfaNI	GCATC	2 cut(s)	816, 1444
SfcI	CTRYAG	1 cut(s)	670
SfuI	TTCGAA	1 cut(s)	1305
SgrDI	CGTCGACG	1 cut(s)	512
SinI	GGWCC	3 cut(s)	475, 871, 1000
SmlI	CTYRAG	1 cut(s)	617
SmoI	CTYRAG	1 cut(s)	617
Sse9I	AATT	6 cut(s)	161, 379, 388, 760, 1015, 1438
SspMI	CTAG	3 cut(s)	29, 798, 1088
SstI	GAGCTC	1 cut(s)	1191
StyI	CCWWGG	3 cut(s)	336, 603, 753
TaaI	ACNGT	3 cut(s)	208, 1259, 1384
TaiI	ACGT	5 cut(s)	347, 459, 513, 780, 1044
TaqII	GACCGA	1 cut(s)	1222
TasI	AATT	6 cut(s)	161, 379, 388, 760, 1015, 1438
TatI	WGTACW	3 cut(s)	72, 501, 666
TauI	GCSGC	6 cut(s)	424, 436, 499, 687, 752, 1294
TfiI	GAWTC	3 cut(s)	4, 184, 427
Tru1I	TTAA	1 cut(s)	804
Tru9I	TTAA	1 cut(s)	804
TscAI	CASTG	1 cut(s)	211
TseFI	GTSAC	3 cut(s)	202, 259, 340
TseI	GCWGC	3 cut(s)	445, 957, 1431
Tsp45I	GTSAC	3 cut(s)	202, 259, 340
TspDTI	ATGAA	3 cut(s)	521, 922, 1004
TspGWI	ACGGA	1 cut(s)	1047
TspRI	CASTG	1 cut(s)	211
Tth111I	GACNNNGTC	2 cut(s)	1004, 1029
VpaK11BI	GGWCC	3 cut(s)	475, 871, 1000
XagI	CCTNNNNNAGG	1 cut(s)	866
XapI	RAATTY	4 cut(s)	161, 388, 1015, 1438
XmiI	GTMKAC	4 cut(s)	138, 513, 780, 1008
XspI	CTAG	3 cut(s)	29, 798, 1088
ZraI	GACGTC	2 cut(s)	778, 1042
ZrmI	AGTACT	1 cut(s)	668

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.