MD02G1145100.v1.1

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000659
Physical Location & Seq
Reverse (-)
11988536 .. 11991282
2747 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1145100.v1.1.491

Sequence Viewer

Length: 1698 bp
ATGGCTAGCATCTGCCTCCGGAACAGGTTTCTGTGCACGTTCGTGTTTATTTTCTTCGGGTTTCTTGGCTTAATCACTTCTCCTGCTGAAGCTGCCATCAAGAAATACCAGTTTGATGTCCAAGTGAAGAACGTGAGCAGATTGTGCCATGCAAAACCGATTGTTACTGTAAATGGAAGGTTTCCGGGACCTACGATCTATGTCCGAGAAGGTGACAGACTTCAAATCAATGTTACAAACCATGCTCAATACAACATGTCCATTCACTGGCATGGATTGAAGCAGTATCGAAACGGTTGGGCAGACGGACCGGCTTATGTGACGCAATGTCCGATCCAGACCGGAAGTAGCTACACTTACGACTTCAATGTGACAGGGCAAAGAGGAACTCTGTGGTGGCATGCACACATTCTTTGGCTAAGGGCTACAGTCTATGGAGCAATTGTCATCATGCCTAAACAAGGAACTCCATTCCCGTTCGCACAGCCATACAGGGAAACTAAGATTATTCTCGGCGAATGGTGGAATGTCGATGTCGAAACCTTTGTCAACAAAGCAAACAGCTTGGGGTTGCCACCAAACTCGTCAGATGCTCACACTATCAACGGGAAGCCAGGGCCATTGTTTCCTTGCTCTGAGAAACATACTTTTGCAATGGAGGTTGAGCAAGGGAAGACCTATCTCCTCAGAATCATCAATGCCGCTCTCAACGACGAACTTTTCTTCGCCATTGCTGGACACAATCTGACAGTGGTGGAGGTTGATGCACTTTACACCAAACCCTTCACTACTCAAGCCATACTAATCGCACCAGGCCAGACCACAAACGTTTTGGTCAAAGCCAATCAAGCTTCAGGCAGATACTTCATGGCCGCCAGACCTTTCATGGATGCTCCGGTTCCCGTCGACAACAAGACAGCCACAGCCATCTTCCAATACAGAGGAGTTCCGAACACCGTCCTGCCAAGCCTTCCGCAACTGCCTAACCCTAATGACACGTCCTTCGCTTTAAGCTACAATAAGAAGCTCAGAAGCCTAAACAGTCCAAACTTTCCGGCCAATGTGCCTCTCAAAGTCGACCGGAAACTCTTCTACACCATTGGTTTCGGGAAAGAATCGTGCCCTAGTTGCATAAACGGAACAAGATTTGTTGCTTCCTTGAACAACATCTCTTTCGAGATGCCTCAGGTCGGGCTTCTGCAAGCACATTACTTCAATCTCAAAGGGGTGTTCAAAACCGACTTCCCAGATAGGCCTTCAACTGTATTCAACTACACTGGTGCACCACTCACAGCCAATCTTGGGACATCAACTGGAACTAGGCTGAGCAAGATTGCTTTCAATTCGACAGTTGAGCTGGTTTTACAAGACACGAATCTCCTAACTGTGGAGTCGCATCCGTTCCATCTCCACGGATACAACTTCTTTGTTGTTGGAAATGGGATTGGGAATTTTGATCCTGCCAAGGAACCTGCTAAGTACAACTTGGTTGATCCTGTTGAGAGAAATACAGTTGGAGTTCCCACAGGTGGTTGGACCGCCATTCGATTTAGAGCTGATAATCCAGGCGTTTGGTTCATACACTGTCACTTGGAGCTTCACACTGGCTGGGGACTGAAAACAGCTTTCGTCGTAGAAGATGGACCAGGATCGGATCAATCTGTTCTGCCTCCGCCTAAGGATCTTCCGCCTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

566

Amino Acids

62.69

Weight (kDa)

8.96

Isoelectric Point (pI)

33.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 40 - 154 5.6e-44 Multicopper oxidase
Cu-oxidase PF00394 167 - 315 2.1e-44 Multicopper oxidase
Cu-oxidase_2 PF07731 415 - 547 7.1e-42 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000659)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G03260
fragaria_vesca FvH4_1g13060 FvH4_2g08670 FvH4_2g08670 FvH4_2g08670 FvH4_2g08840 FvH4_2g08860 FvH4_2g09061
malus_domestica MD02G1145100.v1.1 MD05G1064600.v1.1 MD10G1073700.v1.1 MD15G1258600.v1.1 MD15G1259300.v1.1
prunus_persica Prupe.6G177700_v2.0.a1 Prupe.7G156500_v2.0.a1 Prupe.8G095000_v2.0.a1 Prupe.8G095400_v2.0.a1 Prupe.8G097000_v2.0.a1
pyrus_communis pycom02g11490 pycom05g05550 pycom15g22710
rosa_chinensis RchiOBHm_Chr2g0101231 RchiOBHm_Chr6g0264061 RchiOBHm_Chr6g0264101 RchiOBHm_Chr6g0264311 RchiOBHm_Chr6g0264361 RchiOBHm_Chr6g0264441 RchiOBHm_Chr6g0264891
rosa_laevigata RLG00000014229 RLG00000014253 RLG00000014265 RLG00000014267 RLG00000014269 RLG00000014291 RLG00000014292 RLG00000014316 RLG00000017063
rosa_multiflora Rmu_co8391921.1_g000001 Rmu_co8519839.1_g000001 Rmu_sc0000546.1_g000055 Rmu_sc0002305.1_g000003 Rmu_sc0004470.1_g000004 Rmu_sc0006314.1_g000014 Rmu_sc0009807.1_g000001 Rmu_sc0010483.1_g000004
rosa_roxburghii Rroxscaffold_175G00432050 Rroxscaffold_176G00431400 Rroxscaffold_176G00431740 Rroxscaffold_176G00431790 Rroxscaffold_7G00203140 Rroxscaffold_7G00203620
rosa_rugosa Rorug02G0100200 Rorug06G0007600 Rorug06G0012500 Rorug06G0012600 Rorug06G0014100
rosa_samantha Rh2BG150700 Rh2DG150800 Rh6AG128900 Rh6AG131700 Rh6BG124500 Rh6BG124800 Rh6BG128400 Rh6BG129400 Rh6BG132600 Rh6CG123100 Rh6DG110500 Rh6DG113900 Rh6DG114900 Rh6DG118200
rosa_wichuraiana Rw2G011330 Rw6G011140 Rw6G011160 Rw6G011480 Rw6G011540 Rw6G011680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1480
AccB7I CCANNNNNTGG 1 cut(s) 267
AccBSI CCGCTC 1 cut(s) 704
AccI GTMKAC 2 cut(s) 906, 1077
AccIII TCCGGA 1 cut(s) 18
AciI CCGC 6 cut(s) 702, 873, 974, 1539, 1673, 1688
AclI AACGTT 1 cut(s) 828
AclWI GGATC 6 cut(s) 328, 1451, 1487, 1657, 1662, 1689
AcoI YGGCCR 2 cut(s) 870, 1056
AcsI RAATTY 1 cut(s) 1450
AcuI CTGAAG 2 cut(s) 108, 837
AfaI GTAC 1 cut(s) 1481
AfiI CCNNNNNNNGG 6 cut(s) 267, 461, 1190, 1302, 1387, 1529
AflIII ACRYGT 2 cut(s) 255, 996
AgsI TTSAA 9 cut(s) 224, 280, 367, 1162, 1216, 1234, 1260, 1270, 1342
AhdI GACNNNNNGTC 1 cut(s) 327
AjiI CACGTC 1 cut(s) 999
AjnI CCWGG 4 cut(s) 613, 811, 1564, 1645
AleI CACNNNNGTG 1 cut(s) 41
Alw21I GWGCWC 2 cut(s) 38, 1285
Alw44I GTGCAC 2 cut(s) 34, 1281
AlwI GGATC 6 cut(s) 328, 1451, 1487, 1657, 1662, 1689
Aor13HI TCCGGA 1 cut(s) 18
AoxI GGCC 5 cut(s) 617, 814, 870, 1056, 1253
ApaLI GTGCAC 2 cut(s) 34, 1281
ApeKI GCWGC 1 cut(s) 92
ApoI RAATTY 1 cut(s) 1450
ArsI GACNNNNNNTTYG 4 cut(s) 531, 563, 986, 1018
Asp700I GAANNNNTTC 1 cut(s) 1088
AspS9I GGNCC 5 cut(s) 188, 308, 617, 1536, 1643
AsuC2I CCSGG 1 cut(s) 186
AsuHPI GGTGA 1 cut(s) 224
AsuNHI GCTAGC 1 cut(s) 5
AvaII GGWCC 4 cut(s) 188, 308, 1536, 1643
AxyI CCTNAGG 2 cut(s) 1185, 1677
BaeGI GKGCMC 3 cut(s) 38, 1124, 1285
BbsI GAAGAC 1 cut(s) 680
Bbv12I GWGCWC 2 cut(s) 38, 1285
BbvI GCAGC 1 cut(s) 79
BccI CCATC 4 cut(s) 104, 935, 1413, 1634
BciT130I CCWGG 4 cut(s) 615, 813, 1566, 1647
BciVI GTATCC 1 cut(s) 1409
BcnI CCSGG 1 cut(s) 186
BfaI CTAG 3 cut(s) 6, 1125, 1320
BfmI CTRYAG 1 cut(s) 426
BfuAI ACCTGC 1 cut(s) 1480
BfuI GTATCC 1 cut(s) 1409
BisI GCNGC 3 cut(s) 93, 702, 873
BlpI GCTNAGC 1 cut(s) 1325
BlsI GCNGC 3 cut(s) 94, 703, 874
Bme1390I CCNGG 5 cut(s) 186, 615, 813, 1566, 1647
Bme18I GGWCC 4 cut(s) 188, 308, 1536, 1643
BmeRI GACNNNNNGTC 1 cut(s) 327
BmgBI CACGTC 1 cut(s) 999
BmgT120I GGNCC 5 cut(s) 188, 308, 617, 1536, 1643
BmiI GGNNCC 3 cut(s) 189, 900, 1470
BmrFI CCNGG 5 cut(s) 186, 615, 813, 1566, 1647
BmsI GCATC 6 cut(s) 18, 580, 754, 880, 1170, 1405
BmtI GCTAGC 1 cut(s) 9
BpiI GAAGAC 1 cut(s) 680
Bpu10I CCTNAGC 1 cut(s) 419
Bpu1102I GCTNAGC 1 cut(s) 1325
BpuEI CTTGAG 1 cut(s) 777
BpuMI CCSGG 1 cut(s) 186
BsaJI CCNNGG 3 cut(s) 614, 1411, 1464
BsaWI WCCGGW 4 cut(s) 18, 341, 895, 1080
BsaXI ACNNNNNCTCC 2 cut(s) 429, 459
Bsc4I CCNNNNNNNGG 6 cut(s) 267, 461, 1190, 1302, 1387, 1529
Bse118I RCCGGY 1 cut(s) 310
Bse1I ACTGG 5 cut(s) 109, 272, 1282, 1318, 1609
Bse21I CCTNAGG 2 cut(s) 1185, 1677
Bse3DI GCAATG 3 cut(s) 332, 660, 729
BseAI TCCGGA 1 cut(s) 18
BseBI CCWGG 4 cut(s) 615, 813, 1566, 1647
BseDI CCNNGG 3 cut(s) 614, 1411, 1464
BseGI GGATG 2 cut(s) 895, 1396
BseLI CCNNNNNNNGG 6 cut(s) 267, 461, 1190, 1302, 1387, 1529
BseMI GCAATG 3 cut(s) 332, 660, 729
BseMII CTCAG 5 cut(s) 627, 700, 1042, 1199, 1316
BseNI ACTGG 5 cut(s) 109, 272, 1282, 1318, 1609
BseRI GAGGAG 2 cut(s) 674, 957
BseSI GKGCMC 3 cut(s) 38, 1124, 1285
BseXI GCAGC 1 cut(s) 79
BseYI CCCAGC 1 cut(s) 1608
Bsh1285I CGRYCG 1 cut(s) 1081
BshFI GGCC 5 cut(s) 619, 816, 872, 1058, 1255
BsiEI CGRYCG 1 cut(s) 1081
BsiHKAI GWGCWC 2 cut(s) 38, 1285
BsiSI CCGG 7 cut(s) 19, 185, 311, 342, 896, 1055, 1081
BslFI GGGAC 3 cut(s) 201, 1318, 1626
BslI CCNNNNNNNGG 6 cut(s) 267, 461, 1190, 1302, 1387, 1529
BsmFI GGGAC 3 cut(s) 201, 1318, 1626
BsnI GGCC 5 cut(s) 619, 816, 872, 1058, 1255
Bsp1286I GDGCHC 3 cut(s) 38, 1124, 1285
Bsp13I TCCGGA 1 cut(s) 18
Bsp143I GATC 7 cut(s) 195, 333, 1456, 1492, 1649, 1654, 1681
Bsp1720I GCTNAGC 1 cut(s) 1325
BspACI CCGC 6 cut(s) 702, 873, 974, 1539, 1673, 1688
BspANI GGCC 5 cut(s) 619, 816, 872, 1058, 1255
BspCNI CTCAG 5 cut(s) 628, 699, 1041, 1198, 1317
BspEI TCCGGA 1 cut(s) 18
BspLI GGNNCC 3 cut(s) 189, 900, 1470
BspMI ACCTGC 1 cut(s) 1480
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 6 cut(s) 328, 1451, 1487, 1657, 1662, 1689
BsrBI CCGCTC 1 cut(s) 704
BsrDI GCAATG 3 cut(s) 332, 660, 729
BsrFI RCCGGY 1 cut(s) 310
BsrI ACTGG 5 cut(s) 109, 272, 1282, 1318, 1609
BssAI RCCGGY 1 cut(s) 310
BssECI CCNNGG 3 cut(s) 614, 1411, 1464
BssMI GATC 7 cut(s) 195, 333, 1456, 1492, 1649, 1654, 1681
BssT1I CCWWGG 1 cut(s) 1464
Bst2UI CCWGG 4 cut(s) 615, 813, 1566, 1647
Bst6I CTCTTC 1 cut(s) 1094
BstAPI GCANNNNNTGC 1 cut(s) 144
BstC8I GCNNGC 3 cut(s) 7, 402, 1203
BstDEI CTNAG 9 cut(s) 419, 501, 636, 686, 1028, 1185, 1325, 1476, 1677
BstDSI CCRYGG 1 cut(s) 1411
BstENI CCTNNNNNAGG 1 cut(s) 459
BstF5I GGATG 2 cut(s) 895, 1396
BstKTI GATC 7 cut(s) 198, 336, 1459, 1495, 1652, 1657, 1684
BstMBI GATC 7 cut(s) 195, 333, 1456, 1492, 1649, 1654, 1681
BstMCI CGRYCG 1 cut(s) 1081
BstMWI GCNNNNNNNGC 4 cut(s) 92, 144, 848, 1128
BstNI CCWGG 4 cut(s) 615, 813, 1566, 1647
BstNSI RCATGY 2 cut(s) 259, 404
BstSCI CCNGG 5 cut(s) 184, 613, 811, 1564, 1645
BstSFI CTRYAG 1 cut(s) 426
BstSLI GKGCMC 3 cut(s) 38, 1124, 1285
BstV1I GCAGC 1 cut(s) 79
BstV2I GAAGAC 1 cut(s) 680
BstX2I RGATCY 1 cut(s) 1681
BstXI CCANNNNNNTGG 1 cut(s) 1572
BstYI RGATCY 1 cut(s) 1681
Bsu36I CCTNAGG 2 cut(s) 1185, 1677
BsuI GTATCC 1 cut(s) 1409
BsuRI GGCC 5 cut(s) 619, 816, 872, 1058, 1255
BtgI CCRYGG 1 cut(s) 1411
BtrI CACGTC 1 cut(s) 999
BtsCI GGATG 2 cut(s) 895, 1396
BtsIMutI CAGTG 5 cut(s) 265, 756, 1275, 1582, 1602
BveI ACCTGC 1 cut(s) 1480
Cac8I GCNNGC 3 cut(s) 7, 402, 1203
Cfr10I RCCGGY 1 cut(s) 310
Cfr13I GGNCC 5 cut(s) 188, 308, 617, 1536, 1643
CpoI CGGWCCG 1 cut(s) 308
CseI GACGC 1 cut(s) 331
Csp6I GTAC 1 cut(s) 1480
CspI CGGWCCG 1 cut(s) 308
CviAII CATG 8 cut(s) 149, 242, 256, 272, 401, 451, 868, 886
CviQI GTAC 1 cut(s) 1480
DdeI CTNAG 9 cut(s) 419, 501, 636, 686, 1028, 1185, 1325, 1476, 1677
DpnI GATC 7 cut(s) 197, 335, 1458, 1494, 1651, 1656, 1683
DpnII GATC 7 cut(s) 195, 333, 1456, 1492, 1649, 1654, 1681
DriI GACNNNNNGTC 1 cut(s) 327
EaeI YGGCCR 2 cut(s) 870, 1056
Eam1104I CTCTTC 1 cut(s) 1094
Eam1105I GACNNNNNGTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 1094
EciI GGCGGA 2 cut(s) 1662, 1677
Eco130I CCWWGG 1 cut(s) 1464
Eco147I AGGCCT 1 cut(s) 1255
Eco47I GGWCC 4 cut(s) 188, 308, 1536, 1643
Eco57I CTGAAG 2 cut(s) 108, 837
Eco81I CCTNAGG 2 cut(s) 1185, 1677
EcoNI CCTNNNNNAGG 1 cut(s) 459
EcoO109I RGGNCCY 1 cut(s) 188
EcoRII CCWGG 4 cut(s) 613, 811, 1564, 1645
EcoT14I CCWWGG 1 cut(s) 1464
ErhI CCWWGG 1 cut(s) 1464
FaeI CATG 8 cut(s) 152, 245, 259, 275, 404, 454, 871, 889
FalI AAGNNNNNCTT 2 cut(s) 1469, 1501
FaqI GGGAC 3 cut(s) 201, 1318, 1626
FatI CATG 8 cut(s) 148, 241, 255, 271, 400, 450, 867, 885
FblI GTMKAC 2 cut(s) 906, 1077
Fnu4HI GCNGC 3 cut(s) 93, 702, 873
FokI GGATG 2 cut(s) 902, 1383
Fsp4HI GCNGC 3 cut(s) 93, 702, 873
FspBI CTAG 3 cut(s) 6, 1125, 1320
GluI GCNGC 3 cut(s) 93, 702, 873
GsaI CCCAGC 1 cut(s) 1612
HaeIII GGCC 5 cut(s) 619, 816, 872, 1058, 1255
HapII CCGG 7 cut(s) 19, 185, 311, 342, 896, 1055, 1081
HgaI GACGC 1 cut(s) 331
Hin1II CATG 8 cut(s) 152, 245, 259, 275, 404, 454, 871, 889
HincII GTYRAC 3 cut(s) 550, 907, 1078
HindII GTYRAC 3 cut(s) 550, 907, 1078
HindIII AAGCTT 1 cut(s) 849
HinfI GANTC 4 cut(s) 690, 1115, 1375, 1391
HpaII CCGG 7 cut(s) 19, 185, 311, 342, 896, 1055, 1081
HphI GGTGA 1 cut(s) 224
Hpy166II GTNNAC 5 cut(s) 36, 550, 907, 1078, 1283
Hpy188I TCNGA 9 cut(s) 206, 333, 589, 637, 689, 747, 951, 1031, 1654
Hpy188III TCNNGA 5 cut(s) 19, 100, 337, 1108, 1177
Hpy8I GTNNAC 5 cut(s) 36, 550, 907, 1078, 1283
Hpy99I CGWCG 3 cut(s) 716, 908, 1634
HpyAV CCTTC 6 cut(s) 171, 203, 793, 980, 1012, 1266
HpyCH4IV ACGT 4 cut(s) 38, 132, 828, 998
HpyCH4V TGCA 8 cut(s) 36, 152, 404, 653, 767, 1131, 1201, 1283
HpyF10VI GCNNNNNNNGC 4 cut(s) 92, 144, 848, 1128
HpyF3I CTNAG 9 cut(s) 419, 501, 636, 686, 1028, 1185, 1325, 1476, 1677
HpySE526I ACGT 4 cut(s) 38, 132, 828, 998
Hsp92II CATG 8 cut(s) 152, 245, 259, 275, 404, 454, 871, 889
Kpn2I TCCGGA 1 cut(s) 18
Kzo9I GATC 7 cut(s) 195, 333, 1456, 1492, 1649, 1654, 1681
LmnI GCTCC 3 cut(s) 437, 898, 1594
Lsp1109I GCAGC 1 cut(s) 79
LweI GCATC 6 cut(s) 18, 580, 754, 880, 1170, 1405
MaeI CTAG 3 cut(s) 6, 1125, 1320
MaeII ACGT 4 cut(s) 38, 132, 828, 998
MaeIII GTNAC 6 cut(s) 163, 212, 232, 319, 370, 1586
MalI GATC 7 cut(s) 197, 335, 1458, 1494, 1651, 1656, 1683
MbiI CCGCTC 1 cut(s) 704
MboI GATC 7 cut(s) 195, 333, 1456, 1492, 1649, 1654, 1681
MboII GAAGA 8 cut(s) 46, 139, 685, 715, 922, 1081, 1649, 1676
MfeI CAATTG 1 cut(s) 441
MflI RGATCY 1 cut(s) 1681
MhlI GDGCHC 3 cut(s) 38, 1124, 1285
MluCI AATT 3 cut(s) 441, 1342, 1450
MlyI GAGTC 1 cut(s) 1400
MmeI TCCRAC 3 cut(s) 1414, 1495, 1514
MnlI CCTC 9 cut(s) 26, 377, 652, 695, 751, 935, 1077, 1194, 1680
MroI TCCGGA 1 cut(s) 18
MroXI GAANNNNTTC 1 cut(s) 1088
MseI TTAA 2 cut(s) 71, 1010
MslI CAYNNNNRTG 2 cut(s) 41, 270
MspI CCGG 7 cut(s) 19, 185, 311, 342, 896, 1055, 1081
MspR9I CCNGG 5 cut(s) 186, 615, 813, 1566, 1647
MunI CAATTG 1 cut(s) 441
MvaI CCWGG 4 cut(s) 615, 813, 1566, 1647
MwoI GCNNNNNNNGC 4 cut(s) 92, 144, 848, 1128
NciI CCSGG 1 cut(s) 186
NdeII GATC 7 cut(s) 195, 333, 1456, 1492, 1649, 1654, 1681
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 8 cut(s) 152, 245, 259, 275, 404, 454, 871, 889
NlaIV GGNNCC 3 cut(s) 189, 900, 1470
NmeAIII GCCGAG 1 cut(s) 492
NmuCI GTSAC 4 cut(s) 212, 319, 370, 1586
NspI RCATGY 2 cut(s) 259, 404
OliI CACNNNNGTG 1 cut(s) 41
PaeI GCATGC 1 cut(s) 404
PceI AGGCCT 1 cut(s) 1255
PciI ACATGT 1 cut(s) 255
PcsI WCGNNNNNNNCGW 1 cut(s) 329
PdmI GAANNNNTTC 1 cut(s) 1088
PfeI GAWTC 3 cut(s) 690, 1115, 1375
PflMI CCANNNNNTGG 1 cut(s) 267
PfoI TCCNGGA 1 cut(s) 184
PkrI GCNGC 3 cut(s) 94, 703, 874
PleI GAGTC 1 cut(s) 1399
PpsI GAGTC 1 cut(s) 1399
PpuMI RGGWCCY 1 cut(s) 188
PscI ACATGT 1 cut(s) 255
Psp1406I AACGTT 1 cut(s) 828
Psp5II RGGWCCY 1 cut(s) 188
Psp6I CCWGG 4 cut(s) 613, 811, 1564, 1645
PspFI CCCAGC 1 cut(s) 1608
PspGI CCWGG 4 cut(s) 613, 811, 1564, 1645
PspN4I GGNNCC 3 cut(s) 189, 900, 1470
PspPI GGNCC 5 cut(s) 188, 308, 617, 1536, 1643
PspPPI RGGWCCY 1 cut(s) 188
PsuI RGATCY 1 cut(s) 1681
RsaI GTAC 1 cut(s) 1481
RsaNI GTAC 1 cut(s) 1480
RseI CAYNNNNRTG 2 cut(s) 41, 270
Rsr2I CGGWCCG 1 cut(s) 308
RsrII CGGWCCG 1 cut(s) 308
SalI GTCGAC 2 cut(s) 905, 1076
SaqAI TTAA 2 cut(s) 71, 1010
SatI GCNGC 3 cut(s) 93, 702, 873
Sau3AI GATC 7 cut(s) 195, 333, 1456, 1492, 1649, 1654, 1681
Sau96I GGNCC 5 cut(s) 188, 308, 617, 1536, 1643
SchI GAGTC 1 cut(s) 1400
ScrFI CCNGG 5 cut(s) 186, 615, 813, 1566, 1647
SduI GDGCHC 3 cut(s) 38, 1124, 1285
SfaNI GCATC 6 cut(s) 18, 580, 754, 880, 1170, 1405
SfcI CTRYAG 1 cut(s) 426
SinI GGWCC 4 cut(s) 188, 308, 1536, 1643
SmiMI CAYNNNNRTG 2 cut(s) 41, 270
SmlI CTYRAG 1 cut(s) 792
SmoI CTYRAG 1 cut(s) 792
SphI GCATGC 1 cut(s) 404
Sse9I AATT 3 cut(s) 441, 1342, 1450
SseBI AGGCCT 1 cut(s) 1255
SsiI CCGC 6 cut(s) 702, 873, 974, 1539, 1673, 1688
SspMI CTAG 3 cut(s) 6, 1125, 1320
StuI AGGCCT 1 cut(s) 1255
StyD4I CCNGG 5 cut(s) 184, 613, 811, 1564, 1645
StyI CCWWGG 1 cut(s) 1464
TaiI ACGT 4 cut(s) 41, 135, 831, 1001
TaqI TCGA 8 cut(s) 289, 531, 537, 906, 1077, 1176, 1346, 1546
TasI AATT 3 cut(s) 441, 1342, 1450
TatI WGTACW 1 cut(s) 1479
TauI GCSGC 2 cut(s) 704, 875
TfiI GAWTC 3 cut(s) 690, 1115, 1375
Tru1I TTAA 2 cut(s) 71, 1010
Tru9I TTAA 2 cut(s) 71, 1010
TscAI CASTG 5 cut(s) 272, 756, 1282, 1589, 1609
TseFI GTSAC 4 cut(s) 212, 319, 370, 1586
TseI GCWGC 1 cut(s) 92
Tsp45I GTSAC 4 cut(s) 212, 319, 370, 1586
TspDTI ATGAA 3 cut(s) 856, 874, 1567
TspGWI ACGGA 4 cut(s) 321, 1152, 1389, 1428
TspRI CASTG 5 cut(s) 272, 756, 1282, 1589, 1609
Van91I CCANNNNNTGG 1 cut(s) 267
VneI GTGCAC 2 cut(s) 34, 1281
VpaK11BI GGWCC 4 cut(s) 188, 308, 1536, 1643
XagI CCTNNNNNAGG 1 cut(s) 459
XapI RAATTY 1 cut(s) 1450
XceI RCATGY 2 cut(s) 259, 404
XcmI CCANNNNNNNNNTGG 2 cut(s) 829, 883
XmiI GTMKAC 2 cut(s) 906, 1077
XmnI GAANNNNTTC 1 cut(s) 1088
XspI CTAG 3 cut(s) 6, 1125, 1320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.