MD15G1258600.v1.1

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000659
Physical Location & Seq
Forward (+)
21967291 .. 21969893
2603 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1258600.v1.1.491

Sequence Viewer

Length: 1698 bp
ATGGCTAGCAGCTGCCTCTGGAAAAGATTTTTATGCACGTTCGTGTTTGTCTTTTGCGGTTTTCTTGGCTTAATCACTTCTCCAGCTGAAGCTGCTATTAAGAAATACCAGTTTGATGTTCAAGTAAAGAAGGTGAGCAGATTGTGCCATGCAAAACCGATTGTTACTGTAAATGGCGGATTTCCAGGGCCTACGATCTATGTCCGGGAAGGTGACAGACTTCAAATCAATGTCACAAACCATGCTCAATACAACATGTCAATTCACTGGCATGGATTGAAGCAGTACCGAAATGGTTGGGCAGATGGACCAGCTTATGTGACCCAATGTCCGATCCAGACCGGAAGTAGCTACACATACGACTTCAATGTGACAGGGCAAAGAGGAACTTTGTGGTGGCATGCACATATTCTTTGGCTAAGGGCTACTGTCTATGGAGCAATTGTGATCATGCCTAAACAAAGAACTCCATTCCCATTCCCACAGCCGTATAGGGAAACTGAGATTATACTTGGAGAATGGTGGAATGTGGATGTGGAAACCTTTGTCAACAAGGCAAACAGCTTGGGATTGCCACCAAACTCCTCAGATGCTCACACCATCAATGGCAAGCCAGGGCCATTGTTTCCTTGCTCTGAGAAACATACTTTTGCAATGGAGGTTGAGCAAGGGAAGACCTATCTCCTCAGAATCATCAACGCTGCTCTCAATGACGAACTTTTCTTCTGCATTGCTGGACACAACCTGACAGTGGTGGAGGTCGATGCAGTTTACACCAAACCCTTCACTACTCAAGCCATACTAATTGCACCAGGCCAGACCACAAACGTTTTGGTCAAAGCCAATCAAGCTTCAGGCAGATACTTCATGGCTGCTAGGCCTTTCATGGATGCTCCAGTTCCCGTAGACAACAAGACAGCCACTGCCATTCTCCAATACCAAGGAGTTCTGAACACCATCCTGCCAAGCCTTCCTCAATTGCCTAACCCTAACGACACGTCCTTCGCTTTAAGCTACAACAAGAAGCTCAGAAGCCTAAACACTCCAAACTTTCCGGCCAATGTGCCTCTCAAAGTCGACCGGAAACTCTTCTACACCATTGGTTTCGGGAAAGAATCGTGCCCTAGTTGCATCAACGGAACACGATTTGTTGCTTCCTTGAACAACATCTCTTTCGAAATGCCTCAGGTCGGTCTTCTTCAAGCACATTACTTCAATCTCAAAGGGGTGTTCAAAACCGACTTCCCTGATAGGCCTTCAACTCCTTTCAACTACACTGGTGCACCACTCACAGCCAATCTTGGGACATCAACTGGAACTAGACTGAGCAAGATTGCGTTCAATTCGACGGTTGAGCTTGTTTTGCAAGACACAAATCTCCTAACAGTGGAGTCGCATCCGTTCCATCTCCACGGATACAACTTCTTTGTTGTTGGAACTGGGATTGGGAACTTTGATCCTGCCAAGGACCCTGCTAAGTACAACTTAGTTGATCCTGTTGAGAGAAATACAGTCGGTGTTCCCACAGGTGGTTGGACTGCCATTCGGTTTAGAGCTGATAATCCAGGTGTTTGGTTCATGCACTGTCATTTGGAGCTTCATACCGGGTGGGGATTGAAGATGGCATTTGTTGTAGAAGATGGACCGGGATTAGATCTATCGGTCCTGCCTCCGCCTAAGGATCTTCCACCGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

566

Amino Acids

62.72

Weight (kDa)

8.72

Isoelectric Point (pI)

37.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 40 - 154 9.6e-44 Multicopper oxidase
Cu-oxidase PF00394 167 - 315 4.3e-45 Multicopper oxidase
Cu-oxidase_2 PF07731 415 - 547 6.1e-43 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000659)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G03260
fragaria_vesca FvH4_1g13060 FvH4_2g08670 FvH4_2g08670 FvH4_2g08670 FvH4_2g08840 FvH4_2g08860 FvH4_2g09061
malus_domestica MD02G1145100.v1.1 MD05G1064600.v1.1 MD10G1073700.v1.1 MD15G1258600.v1.1 MD15G1259300.v1.1
prunus_persica Prupe.6G177700_v2.0.a1 Prupe.7G156500_v2.0.a1 Prupe.8G095000_v2.0.a1 Prupe.8G095400_v2.0.a1 Prupe.8G097000_v2.0.a1
pyrus_communis pycom02g11490 pycom05g05550 pycom15g22710
rosa_chinensis RchiOBHm_Chr2g0101231 RchiOBHm_Chr6g0264061 RchiOBHm_Chr6g0264101 RchiOBHm_Chr6g0264311 RchiOBHm_Chr6g0264361 RchiOBHm_Chr6g0264441 RchiOBHm_Chr6g0264891
rosa_laevigata RLG00000014229 RLG00000014253 RLG00000014265 RLG00000014267 RLG00000014269 RLG00000014291 RLG00000014292 RLG00000014316 RLG00000017063
rosa_multiflora Rmu_co8391921.1_g000001 Rmu_co8519839.1_g000001 Rmu_sc0000546.1_g000055 Rmu_sc0002305.1_g000003 Rmu_sc0004470.1_g000004 Rmu_sc0006314.1_g000014 Rmu_sc0009807.1_g000001 Rmu_sc0010483.1_g000004
rosa_roxburghii Rroxscaffold_175G00432050 Rroxscaffold_176G00431400 Rroxscaffold_176G00431740 Rroxscaffold_176G00431790 Rroxscaffold_7G00203140 Rroxscaffold_7G00203620
rosa_rugosa Rorug02G0100200 Rorug06G0007600 Rorug06G0012500 Rorug06G0012600 Rorug06G0014100
rosa_samantha Rh2BG150700 Rh2DG150800 Rh6AG128900 Rh6AG131700 Rh6BG124500 Rh6BG124800 Rh6BG128400 Rh6BG129400 Rh6BG132600 Rh6CG123100 Rh6DG110500 Rh6DG113900 Rh6DG114900 Rh6DG118200
rosa_wichuraiana Rw2G011330 Rw6G011140 Rw6G011160 Rw6G011480 Rw6G011540 Rw6G011680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 906, 1077
AciI CCGC 3 cut(s) 57, 177, 1673
AclI AACGTT 1 cut(s) 828
AclWI GGATC 4 cut(s) 328, 1451, 1487, 1689
AcoI YGGCCR 1 cut(s) 1056
AcuI CTGAAG 2 cut(s) 108, 837
AfaI GTAC 2 cut(s) 287, 1481
AfiI CCNNNNNNNGG 5 cut(s) 751, 1190, 1302, 1387, 1529
AflIII ACRYGT 2 cut(s) 255, 996
AhdI GACNNNNNGTC 1 cut(s) 327
AjiI CACGTC 1 cut(s) 999
AjnI CCWGG 4 cut(s) 184, 613, 811, 1564
AleI CACNNNNGTG 1 cut(s) 41
Alw21I GWGCWC 1 cut(s) 1285
Alw44I GTGCAC 1 cut(s) 1281
AlwI GGATC 4 cut(s) 328, 1451, 1487, 1689
AlwNI CAGNNNCTG 1 cut(s) 923
AoxI GGCC 6 cut(s) 188, 617, 814, 878, 1056, 1253
ApaLI GTGCAC 1 cut(s) 1281
ApeKI GCWGC 5 cut(s) 9, 12, 92, 701, 872
ArsI GACNNNNNNTTYG 2 cut(s) 986, 1018
Asp700I GAANNNNTTC 1 cut(s) 1088
AspS9I GGNCC 6 cut(s) 188, 308, 617, 1468, 1643, 1663
AsuC2I CCSGG 3 cut(s) 206, 1606, 1647
AsuHPI GGTGA 2 cut(s) 145, 224
AsuII TTCGAA 1 cut(s) 1176
AsuNHI GCTAGC 1 cut(s) 5
AvaII GGWCC 4 cut(s) 308, 1468, 1643, 1663
AxyI CCTNAGG 2 cut(s) 1185, 1677
BaeGI GKGCMC 2 cut(s) 1124, 1285
BbsI GAAGAC 2 cut(s) 680, 1187
Bbv12I GWGCWC 1 cut(s) 1285
BbvI GCAGC 4 cut(s) 21, 79, 688, 859
BccI CCATC 6 cut(s) 299, 608, 965, 1413, 1615, 1634
BceAI ACGGC 1 cut(s) 472
BciT130I CCWGG 4 cut(s) 186, 615, 813, 1566
BciVI GTATCC 1 cut(s) 1409
BclI TGATCA 1 cut(s) 447
BcnI CCSGG 3 cut(s) 206, 1606, 1647
BfaI CTAG 4 cut(s) 6, 876, 1125, 1320
BfuI GTATCC 1 cut(s) 1409
BglII AGATCT 1 cut(s) 1654
BisI GCNGC 5 cut(s) 10, 13, 93, 702, 873
BlsI GCNGC 5 cut(s) 11, 14, 94, 703, 874
Bme1390I CCNGG 7 cut(s) 186, 206, 615, 813, 1566, 1606, 1647
Bme18I GGWCC 4 cut(s) 308, 1468, 1643, 1663
BmeRI GACNNNNNGTC 1 cut(s) 327
BmgBI CACGTC 1 cut(s) 999
BmgT120I GGNCC 6 cut(s) 188, 308, 617, 1468, 1643, 1663
BmiI GGNNCC 1 cut(s) 1470
BmrFI CCNGG 7 cut(s) 186, 206, 615, 813, 1566, 1606, 1647
BmrI ACTGGG 1 cut(s) 1449
BmsI GCATC 5 cut(s) 580, 754, 880, 1140, 1405
BmtI GCTAGC 1 cut(s) 9
BmuI ACTGGG 1 cut(s) 1449
BpiI GAAGAC 2 cut(s) 680, 1187
BpmI CTGGAG 2 cut(s) 66, 879
Bpu10I CCTNAGC 1 cut(s) 419
Bpu14I TTCGAA 1 cut(s) 1176
BpuEI CTTGAG 1 cut(s) 777
BpuMI CCSGG 3 cut(s) 206, 1606, 1647
BsaJI CCNNGG 5 cut(s) 185, 614, 940, 1411, 1464
BsaWI WCCGGW 2 cut(s) 341, 1080
BsaXI ACNNNNNCTCC 2 cut(s) 429, 459
Bsc4I CCNNNNNNNGG 5 cut(s) 751, 1190, 1302, 1387, 1529
Bse1I ACTGG 6 cut(s) 109, 272, 896, 1282, 1318, 1444
Bse21I CCTNAGG 2 cut(s) 1185, 1677
Bse3DI GCAATG 2 cut(s) 660, 729
BseBI CCWGG 4 cut(s) 186, 615, 813, 1566
BseDI CCNNGG 5 cut(s) 185, 614, 940, 1411, 1464
BseGI GGATG 4 cut(s) 538, 895, 957, 1396
BseLI CCNNNNNNNGG 5 cut(s) 751, 1190, 1302, 1387, 1529
BseMI GCAATG 2 cut(s) 660, 729
BseMII CTCAG 7 cut(s) 492, 600, 627, 700, 1042, 1199, 1316
BseNI ACTGG 6 cut(s) 109, 272, 896, 1282, 1318, 1444
BseRI GAGGAG 2 cut(s) 574, 674
BseSI GKGCMC 2 cut(s) 1124, 1285
BseXI GCAGC 4 cut(s) 21, 79, 688, 859
Bsh1285I CGRYCG 1 cut(s) 1081
BshFI GGCC 6 cut(s) 190, 619, 816, 880, 1058, 1255
BsiEI CGRYCG 1 cut(s) 1081
BsiHKAI GWGCWC 1 cut(s) 1285
BsiSI CCGG 6 cut(s) 205, 342, 1055, 1081, 1605, 1646
BslFI GGGAC 1 cut(s) 1318
BslI CCNNNNNNNGG 5 cut(s) 751, 1190, 1302, 1387, 1529
BsmFI GGGAC 1 cut(s) 1318
BsnI GGCC 6 cut(s) 190, 619, 816, 880, 1058, 1255
Bsp119I TTCGAA 1 cut(s) 1176
Bsp1286I GDGCHC 2 cut(s) 1124, 1285
Bsp143I GATC 7 cut(s) 195, 333, 447, 1456, 1492, 1654, 1681
BspACI CCGC 3 cut(s) 57, 177, 1673
BspANI GGCC 6 cut(s) 190, 619, 816, 880, 1058, 1255
BspCNI CTCAG 7 cut(s) 493, 599, 628, 699, 1041, 1198, 1317
BspLI GGNNCC 1 cut(s) 1470
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 4 cut(s) 328, 1451, 1487, 1689
BspT104I TTCGAA 1 cut(s) 1176
BsrDI GCAATG 2 cut(s) 660, 729
BsrI ACTGG 6 cut(s) 109, 272, 896, 1282, 1318, 1444
BssECI CCNNGG 5 cut(s) 185, 614, 940, 1411, 1464
BssMI GATC 7 cut(s) 195, 333, 447, 1456, 1492, 1654, 1681
BssT1I CCWWGG 2 cut(s) 940, 1464
Bst2UI CCWGG 4 cut(s) 186, 615, 813, 1566
Bst4CI ACNGT 8 cut(s) 169, 430, 751, 1351, 1387, 1513, 1586, 1692
Bst6I CTCTTC 1 cut(s) 1094
BstAPI GCANNNNNTGC 1 cut(s) 144
BstBI TTCGAA 1 cut(s) 1176
BstC8I GCNNGC 3 cut(s) 7, 402, 611
BstDSI CCRYGG 1 cut(s) 1411
BstF5I GGATG 4 cut(s) 538, 895, 957, 1396
BstKTI GATC 7 cut(s) 198, 336, 450, 1459, 1495, 1657, 1684
BstMBI GATC 7 cut(s) 195, 333, 447, 1456, 1492, 1654, 1681
BstMCI CGRYCG 1 cut(s) 1081
BstMWI GCNNNNNNNGC 5 cut(s) 92, 144, 848, 1128, 1363
BstNI CCWGG 4 cut(s) 186, 615, 813, 1566
BstNSI RCATGY 2 cut(s) 259, 404
BstSCI CCNGG 7 cut(s) 184, 204, 613, 811, 1564, 1604, 1645
BstSLI GKGCMC 2 cut(s) 1124, 1285
BstV1I GCAGC 4 cut(s) 21, 79, 688, 859
BstV2I GAAGAC 2 cut(s) 680, 1187
BstX2I RGATCY 2 cut(s) 1654, 1681
BstXI CCANNNNNNTGG 1 cut(s) 1572
BstYI RGATCY 2 cut(s) 1654, 1681
Bsu36I CCTNAGG 2 cut(s) 1185, 1677
BsuI GTATCC 1 cut(s) 1409
BsuRI GGCC 6 cut(s) 190, 619, 816, 880, 1058, 1255
BtgI CCRYGG 1 cut(s) 1411
BtrI CACGTC 1 cut(s) 999
BtsCI GGATG 4 cut(s) 538, 895, 957, 1396
BtsI GCAGTG 1 cut(s) 921
BtsIMutI CAGTG 6 cut(s) 265, 756, 921, 1275, 1392, 1582
Cac8I GCNNGC 3 cut(s) 7, 402, 611
CaiI CAGNNNCTG 1 cut(s) 923
Cfr13I GGNCC 6 cut(s) 188, 308, 617, 1468, 1643, 1663
Csp6I GTAC 2 cut(s) 286, 1480
CviAII CATG 9 cut(s) 149, 242, 256, 272, 401, 451, 868, 886, 1579
CviQI GTAC 2 cut(s) 286, 1480
DpnI GATC 7 cut(s) 197, 335, 449, 1458, 1494, 1656, 1683
DpnII GATC 7 cut(s) 195, 333, 447, 1456, 1492, 1654, 1681
DriI GACNNNNNGTC 1 cut(s) 327
EaeI YGGCCR 1 cut(s) 1056
Eam1104I CTCTTC 1 cut(s) 1094
Eam1105I GACNNNNNGTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 1094
EciI GGCGGA 2 cut(s) 192, 1662
Eco130I CCWWGG 2 cut(s) 940, 1464
Eco147I AGGCCT 2 cut(s) 880, 1255
Eco47I GGWCC 4 cut(s) 308, 1468, 1643, 1663
Eco57I CTGAAG 2 cut(s) 108, 837
Eco81I CCTNAGG 2 cut(s) 1185, 1677
EcoO109I RGGNCCY 2 cut(s) 188, 1468
EcoRII CCWGG 4 cut(s) 184, 613, 811, 1564
EcoT14I CCWWGG 2 cut(s) 940, 1464
ErhI CCWWGG 2 cut(s) 940, 1464
FaeI CATG 9 cut(s) 152, 245, 259, 275, 404, 454, 871, 889, 1582
FalI AAGNNNNNCTT 4 cut(s) 373, 405, 1469, 1501
FaqI GGGAC 1 cut(s) 1318
FatI CATG 9 cut(s) 148, 241, 255, 271, 400, 450, 867, 885, 1578
FbaI TGATCA 1 cut(s) 447
FblI GTMKAC 2 cut(s) 906, 1077
Fnu4HI GCNGC 5 cut(s) 10, 13, 93, 702, 873
FokI GGATG 4 cut(s) 545, 902, 944, 1383
Fsp4HI GCNGC 5 cut(s) 10, 13, 93, 702, 873
FspBI CTAG 4 cut(s) 6, 876, 1125, 1320
GluI GCNGC 5 cut(s) 10, 13, 93, 702, 873
GsuI CTGGAG 2 cut(s) 66, 879
HaeIII GGCC 6 cut(s) 190, 619, 816, 880, 1058, 1255
HapII CCGG 6 cut(s) 205, 342, 1055, 1081, 1605, 1646
Hin1II CATG 9 cut(s) 152, 245, 259, 275, 404, 454, 871, 889, 1582
HincII GTYRAC 2 cut(s) 550, 1078
HindII GTYRAC 2 cut(s) 550, 1078
HindIII AAGCTT 1 cut(s) 849
HinfI GANTC 3 cut(s) 690, 1115, 1391
HpaII CCGG 6 cut(s) 205, 342, 1055, 1081, 1605, 1646
HphI GGTGA 2 cut(s) 145, 224
Hpy166II GTNNAC 5 cut(s) 550, 772, 907, 1078, 1283
Hpy188I TCNGA 6 cut(s) 333, 589, 637, 689, 951, 1031
Hpy188III TCNNGA 3 cut(s) 19, 337, 1108
Hpy8I GTNNAC 5 cut(s) 550, 772, 907, 1078, 1283
Hpy99I CGWCG 1 cut(s) 1351
HpyAV CCTTC 6 cut(s) 124, 203, 793, 980, 1012, 1266
HpyCH4III ACNGT 8 cut(s) 169, 430, 751, 1351, 1387, 1513, 1586, 1692
HpyCH4IV ACGT 3 cut(s) 38, 828, 998
HpyF10VI GCNNNNNNNGC 5 cut(s) 92, 144, 848, 1128, 1363
HpySE526I ACGT 3 cut(s) 38, 828, 998
Hsp92II CATG 9 cut(s) 152, 245, 259, 275, 404, 454, 871, 889, 1582
Ksp22I TGATCA 1 cut(s) 447
Kzo9I GATC 7 cut(s) 195, 333, 447, 1456, 1492, 1654, 1681
LmnI GCTCC 3 cut(s) 437, 898, 1594
Lsp1109I GCAGC 4 cut(s) 21, 79, 688, 859
LweI GCATC 5 cut(s) 580, 754, 880, 1140, 1405
MaeI CTAG 4 cut(s) 6, 876, 1125, 1320
MaeII ACGT 3 cut(s) 38, 828, 998
MaeIII GTNAC 5 cut(s) 163, 212, 232, 319, 370
MalI GATC 7 cut(s) 197, 335, 449, 1458, 1494, 1656, 1683
MboI GATC 7 cut(s) 195, 333, 447, 1456, 1492, 1654, 1681
MboII GAAGA 8 cut(s) 685, 715, 1081, 1187, 1190, 1630, 1649, 1676
MfeI CAATTG 2 cut(s) 441, 977
MflI RGATCY 2 cut(s) 1654, 1681
MhlI GDGCHC 2 cut(s) 1124, 1285
MluCI AATT 5 cut(s) 261, 441, 804, 977, 1342
MlyI GAGTC 1 cut(s) 1400
MmeI TCCRAC 2 cut(s) 1414, 1514
MroXI GAANNNNTTC 1 cut(s) 1088
MseI TTAA 3 cut(s) 71, 99, 1010
MslI CAYNNNNRTG 2 cut(s) 41, 270
MspA1I CMGCKG 2 cut(s) 12, 86
MspI CCGG 6 cut(s) 205, 342, 1055, 1081, 1605, 1646
MspR9I CCNGG 7 cut(s) 186, 206, 615, 813, 1566, 1606, 1647
MunI CAATTG 2 cut(s) 441, 977
MvaI CCWGG 4 cut(s) 186, 615, 813, 1566
MwoI GCNNNNNNNGC 5 cut(s) 92, 144, 848, 1128, 1363
NciI CCSGG 3 cut(s) 206, 1606, 1647
NdeII GATC 7 cut(s) 195, 333, 447, 1456, 1492, 1654, 1681
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 9 cut(s) 152, 245, 259, 275, 404, 454, 871, 889, 1582
NlaIV GGNNCC 1 cut(s) 1470
NmuCI GTSAC 4 cut(s) 212, 232, 319, 370
NspI RCATGY 2 cut(s) 259, 404
NspV TTCGAA 1 cut(s) 1176
OliI CACNNNNGTG 1 cut(s) 41
PaeI GCATGC 1 cut(s) 404
PceI AGGCCT 2 cut(s) 880, 1255
PciI ACATGT 1 cut(s) 255
PdmI GAANNNNTTC 1 cut(s) 1088
PfeI GAWTC 2 cut(s) 690, 1115
PfoI TCCNGGA 1 cut(s) 204
PkrI GCNGC 5 cut(s) 11, 14, 94, 703, 874
PleI GAGTC 1 cut(s) 1399
PpsI GAGTC 1 cut(s) 1399
PpuMI RGGWCCY 1 cut(s) 1468
PscI ACATGT 1 cut(s) 255
Psp1406I AACGTT 1 cut(s) 828
Psp5II RGGWCCY 1 cut(s) 1468
Psp6I CCWGG 4 cut(s) 184, 613, 811, 1564
PspGI CCWGG 4 cut(s) 184, 613, 811, 1564
PspN4I GGNNCC 1 cut(s) 1470
PspPI GGNCC 6 cut(s) 188, 308, 617, 1468, 1643, 1663
PspPPI RGGWCCY 1 cut(s) 1468
PstNI CAGNNNCTG 1 cut(s) 923
PsuI RGATCY 2 cut(s) 1654, 1681
PvuII CAGCTG 2 cut(s) 12, 86
RsaI GTAC 2 cut(s) 287, 1481
RsaNI GTAC 2 cut(s) 286, 1480
RseI CAYNNNNRTG 2 cut(s) 41, 270
SalI GTCGAC 1 cut(s) 1076
SaqAI TTAA 3 cut(s) 71, 99, 1010
SatI GCNGC 5 cut(s) 10, 13, 93, 702, 873
Sau3AI GATC 7 cut(s) 195, 333, 447, 1456, 1492, 1654, 1681
Sau96I GGNCC 6 cut(s) 188, 308, 617, 1468, 1643, 1663
SchI GAGTC 1 cut(s) 1400
ScrFI CCNGG 7 cut(s) 186, 206, 615, 813, 1566, 1606, 1647
SduI GDGCHC 2 cut(s) 1124, 1285
SfaNI GCATC 5 cut(s) 580, 754, 880, 1140, 1405
SfuI TTCGAA 1 cut(s) 1176
SinI GGWCC 4 cut(s) 308, 1468, 1643, 1663
SmiMI CAYNNNNRTG 2 cut(s) 41, 270
SmlI CTYRAG 1 cut(s) 792
SmoI CTYRAG 1 cut(s) 792
SphI GCATGC 1 cut(s) 404
Sse9I AATT 5 cut(s) 261, 441, 804, 977, 1342
SseBI AGGCCT 2 cut(s) 880, 1255
SsiI CCGC 3 cut(s) 57, 177, 1673
SspMI CTAG 4 cut(s) 6, 876, 1125, 1320
StuI AGGCCT 2 cut(s) 880, 1255
StyD4I CCNGG 7 cut(s) 184, 204, 613, 811, 1564, 1604, 1645
StyI CCWWGG 2 cut(s) 940, 1464
TaaI ACNGT 8 cut(s) 169, 430, 751, 1351, 1387, 1513, 1586, 1692
TaiI ACGT 3 cut(s) 41, 831, 1001
TaqI TCGA 4 cut(s) 762, 1077, 1176, 1346
TaqII GACCGA 2 cut(s) 1181, 1651
TasI AATT 5 cut(s) 261, 441, 804, 977, 1342
TatI WGTACW 1 cut(s) 1479
TfiI GAWTC 2 cut(s) 690, 1115
Tru1I TTAA 3 cut(s) 71, 99, 1010
Tru9I TTAA 3 cut(s) 71, 99, 1010
TscAI CASTG 6 cut(s) 272, 756, 928, 1282, 1392, 1589
TseFI GTSAC 4 cut(s) 212, 232, 319, 370
TseI GCWGC 5 cut(s) 9, 12, 92, 701, 872
Tsp45I GTSAC 4 cut(s) 212, 232, 319, 370
TspDTI ATGAA 4 cut(s) 856, 874, 1567, 1589
TspGWI ACGGA 3 cut(s) 1152, 1389, 1428
TspRI CASTG 6 cut(s) 272, 756, 928, 1282, 1392, 1589
VneI GTGCAC 1 cut(s) 1281
VpaK11BI GGWCC 4 cut(s) 308, 1468, 1643, 1663
XceI RCATGY 2 cut(s) 259, 404
XcmI CCANNNNNNNNNTGG 1 cut(s) 829
XmiI GTMKAC 2 cut(s) 906, 1077
XmnI GAANNNNTTC 1 cut(s) 1088
XspI CTAG 4 cut(s) 6, 876, 1125, 1320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.